BIOTECHNOLOGY

OF
AQUACULTURE
Akhmad Taufiq Mukti

DEPARTMENT of FISH HEALTH MANAGEMENT AND AQUACULTURE

FACULTY of FISHERIES AND MARINE
UNIVERSITAS AIRLANGGA
 APPLICATION STRATEGIES of
AQUACULTURE BIOTECHNOLOGY
Technical Engineering;
Engineering; approach :
Fish
Food / Fed
Diseases
Environment
Rearing / Cultivation

Equipment Engineering;
Engineering; approach :
* Color
* Volume
* Shape
* Models
 FISH approach
BROODSTOCK -- Genetic Evaluation, Hormonal Inject,
Quarantine & Chromosom Manipulate
by Hybridization

EGG – SPERM -- Cryopreserv, Chromosom Manipulate,

Physiology Manipulate & Modern Biotech

LARVAL --- Genetic Evaluation, Physiology Manipulate &

Protect Test

FRY (SEED) -- Genetic Evaluation, Physiology Manipulate,

Quarantine & Protect Test

JUVENILE --- Genetic Evaluation, Hormonal Inject,

Quarantine & Protect Test

FINGERLING --- Genetic Evaluation, Hormonal Inject,

Quarantine & Protect Test
 FOOD / FED approach
BROODSTOCK

EGG - SPERM

LARVAL

FRY (SEED)

JUVENILE

FINGERLING
 DISEASES approach
BROODSTOCK

EGG - SPERM

LARVAL

FRY (SEED)

JUVENILE

FINGERLING
 ENVIRONMENT approach
BROODSTOCK

EGG - SPERM

LARVAL

FRY (SEED)

JUVENILE

FINGERLING
 REARING / CULTIVATION approach
BROODSTOCK

EGG - SPERM

LARVAL

FRY (SEED)

JUVENILE

FINGERLING
 BROODSTOCK MANAGEMENT
• Need of complete information
• Need of high quantity

highest variability / heterogenity

QUALITIES :
* Growth
* SR
* Diseases resistance
* Environment resistance
best * Color & Shape pattern
(ornamental fish)
* Fecundity
* Mature
* Spawn
STRATEGIES to BROODSTOCK MANAGEMENT

1. Genetic Evaluation
order to variability - heterogenity

selection protein / enzyme analysis

morphometric RNA – DNA analysis
karyotyping others (blood etc.)

2. Hormonal Induced
order to fecundity - mature - spawn
oral implantation
injection ablation
hypophyzation laserpunkture
thermal (+) petrichord (+)
3. Quarantine
order to stock care - quality

4. Chromosomal Manipulation by Hybridization

growth - SR - resistance
order to color - shape - sterile
natural monosex
STRATEGIES to EGG - SPERM MANAGEMENTS

1. Cryopreservation
order to
rearing efficiency
broodstock selection
avoid of diseases
continuously gamet stock
easy of transportation
need to anytime
anytime--anywhere

2. Genetic (Chromosomal) Manipulation

growth - SR - resistance
order to
color - shape - sterile

gynogenesis triploidy
androgenesis tetraploidy
3. Fenotype (Physiology) Manipulation
order to growth

sex reversal
hormonal
(monosex culture)
method
oral
immersion/dipping
by broodstock

4. Modern Biotechnology
order to new variety - growth - SR
gene cloning gene expression
gene sequencing disease related
gene transfer (TRANSGENIC)
 FOOD & FED MANAGEMENT
• Need to broodstock (live / maintenance – reproduction)
• Need to larval / seed / juvenile (live – SR – growth)
• Need to fingerling (live – SR – growth – reproduction)

SUPPORT
* quantity
* quality (nutrition)

SUPPLY
* nutrient (EPA, HUFA, AA, Vit A, E, C)
* hormonal (GR)
* alternative (sludge – biogas)

INTEGRATED FARMING SYSTEM

 DISEASE MANAGEMENT
• PREVENTIVE
Vaksin
Immunostimulant
Vitamin C

• CURATIVE - TERAPHY

antibiotics ??? AVOID !!!

 ENVIRONMENT MANAGEMENT
MCS
temperature DO hardness
salinity NH3 brightness
pH NO3 mineral contents

drying
liming
manuring decreased – waters
treatment disease
plankton – live fed
 REARING MANAGEMENT
• POND : - color
- volume
- shape
- models

• WATER QUALITY of INCUBATION (ex: Temperat)

• LIVE FED (early food)
• LIGHT
• NUTRITION / NUTRIENT of ADDITION FED
• STOCK DENSITY
• SIZE – AGE of STOCK
 STRUCTURE--WORKS
STRUCTURE
• RESEARCH JOURNAL --- up to date ( 2015 )
• Format : TITLE
AUTHOR & CO-AUTHOR
ABSTRACT
INTRODUCTION
MATERIAL & METHOD
RESULT
DISCUSSION
CONCLUSION
REFERENCES
 STRUCTURE--WORKS
STRUCTURE
• GROUP : * REPRODUCTION
* GENETIC
* ARTIFICIAL FEED
* LIVE FOOD
* ENVIRONMENT ( WQ )
* DISEASE / VIRUS

COMMODITIES : - FINFISH
- SHELLFISH
- ALGAE
- CRUSTACEA
 STRUCTURE--WORKS
STRUCTURE
• Sent : - Student Name & NIM,
- Title & Abstract of Article/Journal
- Website & Time of Download
(Article/Journal)

• Sent to E-mail : atm.mlg@gmail.com

• TRANSLATE & RESUME
• ORAL PRESENTATION
• PRINT & SOFT COPY (CD) per group