Clinical

Instrumentation Name: Guarin, Allyza Joyce B.

& Yr. & Sec.: 3E
Glassware
Chemistry I Activity No. 1
Date Submitted: 08/10/2020
Rating: ___________________

Instruction: Supply the information asked in the table below.

I. Glassware Picture Feature/s

CHROMATOGRAPHY VIALS -High thermal resistance
-Low Alkali content
-Free from Zinc group elements and
1. Borosilicate glass heavy metals
-Resists heat, corrosion and thermal
shock
-Low coefficient of expansion
CENTRIFUGE TUBE -Resist clouding due to alkali
-Staining
-Harder
2. Alumina-Silicate
-For temperature more than250 c
glass
-30,000 PSI (6 times stronger than
borosilicate)

MEDIA BOTTLE -Provides highly protective

laboratory glassware for handling
materials sensitive to light in the
3. Low Actinic glass 300 –500 nm range
- high thermal resistance with an
amber or red color added as an
integral part of the glass

-Less than 0.2% boron

4. Corning Boron Free -High resistance to alkali
Glass -Thermal resistance less
-strong alkali reactions

II. Instruments Picture Function/Used in Clinical

Chemistry Laboratory

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 5. Beaker -Wide mouthed, straight sided,
cylindrical vessels
-Volumes vary from 5mL to several
liters
-Used for General mixing and non-
volumetric liquid reagents
preparation
6. Test Tube -slender containers that hold small
amounts of liquid
- range between 50 mm to 250 mm
in length and 13 and 20 mm in
width
- also be known as boiling tubes
7. Test Tube Rack - help you organize, store, and
transport test tubes
- support test tubes in vertical wells
that prevent the tube from tilting
- other racks have wells with larger
diameters and allow the tubes to tilt
slightly without spilling their
contents
8. Volumetric Flask
-used to measure volumes much
more precisely than beakers or
Erlenmeyer flasks
- flat bottomed bulb with a
elongated neck calibrated to hold a
set volume at a mark on the neck.

9. Graduated Cylinder
-also known as measuring cylinder
or mixing cylinder
-used to measure the volume of a
liquid
-has a narrow cylindrical shape
-Each marked line on the graduated
cylinder represents the amount of
liquid that has been measured
10. Pipettes -transports a measured volume of
liquid, often as a media dispenser
-designed either to contain (TC) or
to deliver (TD) a specific volume
-TC pipettes will contain a specified
volume but will not dispense the
exact volume, and TD will dispense
the exact volume.

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 11. Aspirator -serves as a vacuum source for
filling reagents through a pipette or
pasteur pipette and also help
control the flow of liquid from the
dropping bottle

12. Centrifuge -device that is used for the

separation of particles from a
solution according to their size,
shape, density, viscosity of the
medium and rotor speed
-separation is achieved by spinning
a vessel containing material at high
speed
13. Centrifuge Tube
-used to contain liquids during
centrifugation
-most centrifuge tubes have conical
bottoms, which help collect any
solid or heavier parts of the sample
being centrifuged
-Range from 0.1mL to more than
100mL
14. Water Bath -used to heat samples in the lab
-maintaining cell lines or heating
flammable chemicals that might
combust if exposed to open flame
-consists of a heating unit, a
stainless-steel chamber that holds
the water and samples, and a
control interface
- circulating water bath: keep a
more even temperature
-shaking water bath: keeps the
samples in motion while they are
heated
15. Spectrophotometer
-used to measure the light
transmitted by a solution to
determine the concentration of the
light-absorbing substance in the
solution.
-Single beam and double beam are
the two major classes of
spectrophotometers
-Linear range of absorption and
spectral bandwidth measurement
are its important features

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 16. Cuvette -aka sample cell, typically has a flat
surface.
-Each cuvette to be tested is filled,
readings are taken, and results are
compared against an acceptable
tolerance
-sealed at one end, and made of a
clear, transparent material such as
plastic, glass, or fused quartz

Questions for Research

1. Differentiate air displacement pipets from positive-displacement pipets.

Air-displacement pipets Positive-displacement pipets

piston-operated devices operates by moving the piston in the
The piston does not come in contact with the pipette tip or barrel, much like a
liquid hypodermic syringe
Use a capillary tip that may be siliconized glass,
A disposable, onetime use polypropylene tip is glass, or plastic.
attached to the pipet barrel Capillary tips are reusable, and carry-over is
negligible if the pipet is properly maintained
Because of carryover concerns, rinsing and
blotting between samples may be required.

2. Explain the working principle of the spectrophotometer in your own words.

Substances absorb light at unique wavelengths the amount of light absorbed is proportional to
the amount of substance that is present. If you could measure what is absorbed of particular
substance (ex. protein) you can use the absorbance value to determine the concentration of the
protein you may have in a certain product.

Reference/s:

Bishop, et al. Clinical Chemistry: Principles and Procedures, Boston: Little Brown, 8th edition, 2018.

Henry, John Bernard. Clinical Diagnosis and Management by Laboratory Methods, 22nd edition.
Philadelphia: W. B. Saunders Company, 2016

(Images are take thru Google Image Search from credible sources)

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