Dionex Product Guide 12aug2008 LPN2002 02 PDF

Contents
Liquid Chromatography Instruments 5 IC & RFIC Column Compartments.................................... 83

IC & RFIC Detectors............................................................ 86
LCi Solutions............................................................ 6 Postcolumn Reaction Systems......................................... 90
Contents
Parallel Analyses................................................................... 6
Tandem Analyses.................................................................. 6
2-D LC Analyses..................................................................... 7
Automated On-Line SPE-LC................................................ 7 Process Analytical Technology 91
Automated Application Switching..................................... 8
Automated Method Scouting............................................. 8 Process Analytical Systems & Software............. 93
Proteomics Preconcentration............................................ 9 DX-800 Process Analyzers................................................ 94
Proteomics Parallel LC......................................................... 9 Chromeleon PA Software.................................................. 94
Proteomics MDLC Solutions............................................. 10
Rapid Separation LC........................................................... 11
UltiMate 3000 LC Systems................................... 13 Mass Spectrometry 95

Basic Systems..................................................................... 14
MS Instruments..................................................... 97
Standard Systems............................................................... 16
Rapid Separation LC Systems........................................... 20 MS Systems and Modules................................................ 98
x2 Dual Systems.................................................................. 22
LC Modules............................................................ 25
Sample Extraction 101
LC Solvent Tray/Degasser................................................. 26
LC Pumps.............................................................................. 28
ASE Accelerated Solvent Extractors................. 103
LC Injectors & Autosamplers............................................ 37
ASE Systems...................................................................... 104
LC Flow Managers.............................................................. 44
LC Column Compartments................................................. 47
LC Detectors......................................................................... 50
ASE Parts & Accessories.................................... 105
Fraction Collection.............................................................. 57 ASE Starter Kits................................................................. 106
ASE Heat Exchanger........................................................ 106
ASE Prep DE and CR......................................................... 106
ASE Cells............................................................................. 106
Ion Chromatography Instruments 61 Filters and Thimbles.......................................................... 108
Vials and Bottles................................................................ 108
RFIC Solutions....................................................... 62
Reagent-Free IC with Eluent Generation........................ 62
Reagent-Free IC with Eluent Regeneration................... 62
IC & RFIC Systems................................................ 63

ICS-3000................................................................................ 64
ICS-2000................................................................................ 66
ICS-1500................................................................................ 68
ICS-1000................................................................................ 69
ICS-900.................................................................................. 70
IC & RFIC Modules................................................ 73

Eluent Generation................................................................ 74
IC & RFIC Pumps.................................................................. 76
IC & RFIC Injectors & Autosamplers............................... 79
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Software 109 IonPac AS9-HC.................................................................. 188
IonPac AS9-SC.................................................................. 190
System Control Software................................... 111 IonPac AS4A-SC............................................................... 192
Chromeleon........................................................................ 112
DCMSLink . ........................................................................... 115 Specialty Anion-Exchange Columns................. 195
IonPac AS7......................................................................... 196
Contents
IonPac AS5......................................................................... 198
LC Columns 117 Cation-Exchange Colums................................... 201

IonPac CS18....................................................................... 202
Reversed-Phase LC Columns............................. 119 IonPac CS17....................................................................... 204
Acclaim 120 C18................................................................. 120 IonPac CS16....................................................................... 206
Acclaim 120 C8................................................................... 122 IonPac CS15....................................................................... 208
Acclaim PolarAdvantage................................................. 124 IonPac CS14....................................................................... 210
Acclaim PolarAdvantage II............................................. 126 IonPac CS12A.................................................................... 211
Acclaim Rapid Separation LC......................................... 128 IonPac CS11....................................................................... 213
IonPac NS1......................................................................... 130 IonPac CS10....................................................................... 214
Specialty LC Columns......................................... 133 Transition Metal Columns.................................. 215

Acclaim Organic Acid...................................................... 134 IonPac CS5A...................................................................... 216
Acclaim Surfactant........................................................... 136
Acclaim Explosives........................................................... 138 Ion-Exclusion Columns....................................... 219
Acclaim Mixed-Mode HILIC-1........................................ 140 IonPac ICE-AS1................................................................. 220
Acclaim Mixed-Mode WAX-1......................................... 142 IonPac ICE-AS6................................................................. 222
Acclaim Mixed-Mode WCX-1......................................... 144 IonPac ICE-Borate............................................................ 224
OmniPac.............................................................................. 146
IC Trap Columns.................................................. 225
Anion Trap Columns.......................................................... 226
Cation Trap Columns......................................................... 227
IC & RFIC Columns 149 Cation Polisher................................................................... 227
MFC-1 Metal-Free............................................................. 228
Hydroxide-Selective Anion-Exchange
Columns............................................................... 151
IC Concentrator Columns................................... 229
IonPac AS24....................................................................... 152
Anion Concentrator Columns.......................................... 230
IonPac Fast Anion IIIA..................................................... 154
Cation Concentrator Columns........................................ 234
IonPac AS21....................................................................... 156
Transition Metal Concentrator Columns...................... 236
IonPac AS20....................................................................... 158
IonPac AS19....................................................................... 160
IonPac AS18....................................................................... 162
IonPac AS17-C................................................................... 164 Bio Columns 237
IonPac AS16....................................................................... 166
IonPac AS15....................................................................... 168 Protein & Peptide Columns................................ 239
IonPac AS11-HC................................................................ 170
ProPac SCX and WCX...................................................... 240
IonPac AS11....................................................................... 172
ProPac SAX and WAX...................................................... 242
IonPac AS10....................................................................... 174
ProPac PA1......................................................................... 244
ProPac HIC......................................................................... 245
Carbonate Eluent Anion-Exchange
ProPac IMAC..................................................................... 246
Columns............................................................... 177
ProSwift RP........................................................................ 248
IonPac AS23....................................................................... 178 ProSwift IEX....................................................................... 250
IonPac AS22....................................................................... 180 Acclaim 300 C18................................................................. 252
IonPac AS14....................................................................... 182 Strong Cation Exchange.................................................. 254
IonPac AS14A.................................................................... 184 Acclaim PepMap............................................................... 255
IonPac AS12A.................................................................... 186 PepSwift.............................................................................. 258
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Nucleic Acid Columns........................................ 261 Dionex Literature................................................. 317
DNAPac PA100.................................................................. 262 General Interest................................................................. 317
DNAPac PA200.................................................................. 263 Product Brochures........................................................... 317
Product Data Sheets........................................................ 317
Amino Acid Columns.......................................... 265 Application Notes.............................................................. 319
AminoPac PA1................................................................... 266 Application Updates......................................................... 325
Contents
AminoPac PA10................................................................. 267 Technical Notes................................................................. 326
Books................................................................................... 327
Carbohydrate Columns...................................... 269
CarboPac MA1................................................................... 270 Service & Technical Support.............................. 328
CarboPac PA1.................................................................... 272 Comprehensive Service Plans....................................... 328
CarboPac PA10.................................................................. 274 Validation Services........................................................... 328
CarboPac PA20.................................................................. 276 Peak Performance Kits.................................................... 328
CarboPac PA100................................................................ 278 Contact Information.......................................................... 328
CarboPac PA200................................................................ 280
Training................................................................ 329
Course Offerings................................................................ 329
Training Options................................................................. 329
Chromatography Accessories 281
Index of Part Numbers....................................... 330
Chemical Suppressors........................................ 283
SRS 300 Self-Regenerating Suppressor....................... 284 Ordering Information.......................................... 337
Atlas Electrolytic Suppressor......................................... 285 Dionex Locations . ............................................................ 337
AMMS-ICE 300 MicroMembrane Suppressor............ 286
MMS 300 MicroMembrane Suppressor...................... 287
SC-CSRS Salt Converter.................................................. 288
CMD 300 Carbohydrate Membrane Desalter.............. 288
Supplies & Accessories...................................... 289

RFIC-Eluent Generation.................................................... 290
RFIC-Eluent Regeneration............................................... 290
CR-TC Continuously-Regenerated Trap Column......... 291
CRD Carbonate Removal Device.................................... 292
OnGuard II Cartridges...................................................... 294
Standards, Reagents, and Eluent Concentrates......... 297
ICS-900 Solutions Kits...................................................... 299
LCi Solutions Kits............................................................... 302
Indices & Appendices 305
Column Selection Guide.................................... 307

Silica Columns................................................................... 307
Polymer Columns.............................................................. 309
Column Specifications........................................ 311

IC Anion Columns.............................................................. 311
IC Cation Columns............................................................. 313
Ion-Exclusion Columns..................................................... 314
Acclaim General and Specialty Columns..................... 314
Bio Columns....................................................................... 314
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Contents
4 www.dionex.com
Liquid Chromatography Instruments

LCi Solutions 6 LC Pumps............................................................... 28
Parallel Analyses................................................................... 6 Related Literature................................................................ 28
Tandem Analyses.................................................................. 6 RSLC Binary Pump.............................................................. 29
2-D LC Analyses..................................................................... 7 Isocratic Analytical Pump................................................. 30
Automated On-Line SPE-LC................................................ 7 Quaternary Analytical Pump............................................. 30
Automated Application Switching..................................... 8 Dual-Gradient Analytical Pump........................................ 31
Automated Method Scouting............................................. 8 Binary Analytical Pump...................................................... 32
Proteomics Preconcentration............................................ 9 Binary Semipreparative Pump......................................... 32
Proteomics Parallel LC......................................................... 9 Quaternary Micro Pump.................................................... 33
Proteomics MDLC Solutions............................................. 10 Dual-Gradient Micro Pump............................................... 34
Rapid Separation LC........................................................... 11 Quaternary Nano/Cap/Micro Pump................................ 35
Dual-Gradient Nano/Cap/Micro Pump........................... 36
LC Injectors & Autosamplers............................... 37

UltiMate 3000 LC Systems 13 Related Literature................................................................ 37
RSLC Autosampler.............................................................. 37
Basic Systems....................................................... 14 Analytical Autosampler...................................................... 38
Related Literature................................................................ 14 Biocompatible Analytical Autosampler.......................... 39
Basic Manual System........................................................ 14 Autosampler Column Compartment................................ 40
Basic Automated System.................................................. 15 Semipreparative Autosampler......................................... 41
Nano/Cap Autosampler..................................................... 42
Standard Systems................................................. 16 Manual Injectors................................................................. 43
Related Literature................................................................ 16
Binary Analytical System.................................................. 16 LC Flow Managers................................................ 44
Quaternary Analytical System.......................................... 17 Related Literature................................................................ 44
Biocompatible Analytical System.................................... 17 Flow Manager with Two 2P-10P Valves......................... 44
Quaternary Micro System................................................. 18 Flow Manager with 2P-10P/2P-6P Valve........................ 45
Binary Semipreparative System...................................... 18 Flow Manager with 2P-10P Valve.................................... 46
Quaternary Nano/Cap/Micro System............................. 19
LC Column Compartments.................................. 47
Rapid Separation LC Systems............................. 20 Related Literature................................................................ 47
Ultimate Speed and Performance................................... 20 RSLC Thermostatted Column Compartment.................. 47
Related Literature................................................................ 21 TCC with Two Switching Valve Drives............................ 49
Ordering Information.......................................................... 21
LC Detectors........................................................... 50
x2 Dual Systems.................................................... 22 Related Literature................................................................ 50
Related Literature................................................................ 22 RSLC Diode Array Detector............................................... 50
x2 Dual Analytical System................................................. 22 RSLC Multiple Wavelength Detector.............................. 51
Biocompatible x2 Dual Analytical System..................... 23 RSLC Variable Wavelength Detector.............................. 52
x2 Dual Micro System........................................................ 23 Variable Wavelength Detector......................................... 54
x2 Dual Nano/Cap/Micro System..................................... 24 Fluorescence Detector...................................................... 55
Refractive Index Detector................................................. 56
Fraction Collection................................................ 57
LC Modules 25 Related Literature................................................................ 57
WPS Microfraction Collection Option............................ 57
LC Solvent Tray/Degasser.................................... 26
Probot MALDI Spotter........................................................ 58
Related Literature................................................................ 26 Foxy Jr. Fraction Collector................................................. 59
Solvent Rack without Degasser....................................... 26
Solvent Racks with Degasser........................................... 26
www.dionex.com 5
LCi Solutions
LCi Solutions
UltiMate® 3000 Intelligent LC (LCi) Solutions combine
optimized system configurations, column chemistries, and
software features to provide turnkey productivity suites for your

toughest challenges. LCi Solutions facilitate multi-dimensional
separations, sample cleanup and analyte enrichment, method
development, as well as proteomics workflows—increasing
sample throughput, sensitivity, and resolution. LCi Solutions are
customized to your applications, setting new standards for LC. Figure 1. Flow scheme for parallel analyses.
UltiMate 3000 LCi Solutions support LC applications over The x2 Dual LC system for parallel analyses costs much less
a wide flow rate range—from analytical to micro and nano than two separate LC systems, so you get double throughput
flows—with sophisticated automation for high instrument utili- without doubling costs.
zation time. LCi Solutions free operator time and increase return
on your system investment. The UltiMate 3000 x2 Dual LC system configuration for paral-
lel analyses includes the SRD-3600 Solvent Rack, the DGP-
LCi Solutions Kits provide preconfigured capillary kits and 3600 Dual-Gradient Pump, the WPS-3000SL In-line Split Loop
comprehensive quick installation guides for fast and convenient Autosampler, the TCC-3200 Thermostatted Column Compart-
implementation. They are available under “Accessories” in the ment, one 2-position 6-port switching valve, and two UV/Vis
Columns & Accessories section. Detectors. This system setup also supports On-Line SPE-LC.
Learn more about LCi Solutions on www.dionex.com under LC

and LC/MS. Contact your local Dionex representative for an
Tandem Analyses
LCi Solution system configuration customized to your applica- Tandem analysis provides another way to shorten your run
tions. (See “Dionex Locations” under Order Information.) For times, utilizing the power of off-line column regeneration.
equipment specifications, please see the LC Modules section.
• Increase throughput for new and existing methods
Parallel Analyses • No revalidation of methods required

• Up to 50% shorter run times, with off-line column regen-
Increase sample throughput without the added time, effort, and
eration
cost of multiple LC instruments,method redevelopment and
revalidation. UltiMate 3000 x2 Dual LC systems provide the • Analytical and micro tandem LC application kits include
functionality of two systems in one footprint. Chromeleon® pro- all required tubing and instructions
vides the brains to automatically switch between applications—
for the productivity of two systems in the time and effort of • Tandem LC wizard in Chromeleon software helps to con-
running one. Double your sample throughput with parallel LC. vert existing methods into tandem LC methods.
• Run two different or identical LC applications in parallel

• Increase throughput for new and existing methods
• No revalidation of methods required
• Supports isocratic and gradient separations
• Analytical and micro parallel LC application kits provide
all required tubing and instructions
In parallel LC mode, one autosampler and one column com- Figure 2. Flow scheme for tandem analyses.
partment are shared between the two independent flow paths
In tandem LC mode, two identical columns are switched
of the UltiMate 3000 x2 Dual LC system with Dual-Gradient
between two flow paths—an analysis flow path and a regen-
Pump (Figure 1). Two detectors record the UV/Vis signal of
eration flow path (Figure 2) to allow column washing and
two LC applications simultaneously. Chromeleon Chromatog-
re-equilibration off-line. While one column is equilibrated, the
raphy Management Software treats the system configuration as
system injects the next sample on the other. This solution saves
two completely independent systems, and manages seamless
the time required to wash and equilibrate a column for the next
autosampler and column compartment sharing.
injection—typically 20–50% of total analysis time.
6 www.dionex.com
2-D LC Analyses
The UltiMate 3000 x2 Dual LC system for Tandem Analyses UV/Vis Detector. This system setup also supports tandem and
includes the SRD-3600 Solvent Rack, DGP-3600 Dual-Gradient On-Line SPE-LC.
Pump, WPS-3000SL In-line Split Loop Autosampler, TCC-
3200 Thermostatted Column Compartment, one 2-position 10-

port switching valve, and a UV/Vis Detector. This system setup Automated On-Line SPE-LC
also supports 2-D LC analyses and On-Line SPE-LC.
Solid phase extraction (SPE) is often used to isolate analytes of
interest from a complex matrix. The UltiMate 3000 x2 Dual LC
2-D LC Analyses system provides the technology for fully automated On-Line
SPE.
The UltiMate 3000 LCi Solution for two-dimensional liquid
chromatography (2-D LC) streamlines separation of analytes in • Direct injection of untreated samples (e.g., plasma, urine,
complex sample mixtures. serum, vegetable oils, and surface water)
• Automated sample cleanup and/or analyte enrichment for
• Supports combinations of two orthogonal chromatographic
unattended sample processing
techniques, for example, IEX and RP-LC
• Elimination of conventional manual sample pretreatment
• Supports combination of affinity chromatography tech-
steps
niques with RP-LC
• Less prone to errors leading to better results
• Provides high peak capacities and separates complex
samples • Reduced operator exposure to hazardous samples
• Analytical 2-D LC application kit includes all required tub- • Increased workload per system for higher return on your
ing and instructions equipment investment
• Easy visualization of the system along with real-time data • Analytical and micro On-Line SPE-LC application kits
monitoring and system control by Chromeleon Software include all required tubing and instructions
In two-dimensional liquid chromatography (2-D LC), samples In On-Line SPE, the sample is first injected on to the SPE col-
are separated, in series, on two columns with different selec- umn for sample fractionation and matrix elimination (Figure 4).
tivities (Figure 3). Compounds with similar selectivities to the Next, the analytes of interest are transferred from the SPE
first column (first dimension) generally elute as a single peak. column to a reversed-phase column for separation, followed by
These compounds are transferred from the first dimension to the detection. The two independent flow paths of the UltiMate 3000
second column (second dimension) and will be separated due to x2 Dual LC system ensure reliable SPE-LC operation.
the change in selectivity.
Figure 4. Flow scheme for automated on-line SPE-LC.
Chromeleon Chromatography Management Software provides

Figure 3. Flow scheme for 2-D LC analyses.
an On-Line SPE-LC wizard to guide you through the steps to
The unique technology provided by the UltiMate 3000 x2 Dual create and optimize an On-Line SPE method. No special train-
LC series systems allows both dimensions to be performed and ing is required—you can use these advanced features as soon
automated in a single system, saving laboratories both money as the UltiMate 3000 x2 Dual LC system is installed in your
and bench space while increasing productivity and performance. laboratory.
The UltiMate 3000 x2 Dual LC system configuration for 2-D The UltiMate 3000 x2 Dual LC system for automated On-Line
LC analyses includes the SRD-3600 Solvent Rack, DGP-3600A SPE-LC includes the SRD-3600 Solvent Rack, DGP-3600
Analytical Dual-Gradient Pump, WPS-3000SL Analytical In- Dual-Gradient Pump, WPS-3000SL In-line Split Loop Au-
line Split Loop Autosampler, TCC-3200 Thermostatted Column tosampler, TCC-3200 Thermostatted Column Compartment,
Compartment, one 2-position 10-port switching valve, and a one 2-position 6-port switching valve, and a UV/Vis Detector.
www.dionex.com 7
LCi Solutions
Automated Application Switching Automated Method Scouting
The UltiMate 3000 x2 Dual LC system makes it possible to The UltiMate 3000 LCi Solution for automated method scout-
automate the switch over from one analytical method to another, ing provides a technique for fast methods development by
even if different columns and mobile phases are used. This tech- automatically testing method parameters, such as pH value of
nique saves the time that would be spent on changing mobile the mobile phase, solvent composition, stationary phase and
phase and column, and flushing and equilibrating the system column temperature.
for the new eluent composition. In addition, it can be performed
overnight or during the weekends, allowing for maximum • Optimized hardware design, with two multi position valves
instrument utilization. The advanced x2 Dual LC system with in the column compartment
Chromeleon Software automates these steps and switches your
• Analytical and micro automated method scouting kits
system from one application to another in a matter of minutes.
include all required tubing and instructions
• Easily switch between applications by clicking on the • Fast and convenient instrument control and method setup
Chromeleon control panel.
• Powerful queries and reporting tools for data processing
• Two independent flow paths support the use of separate and evaluation
columns and mobile phases.
• Chromeleon post processing tools find the optimal method
• Tubing to and from the autosampler can be flushed before in seconds, eliminating the need for manual data assess-
switching to another application. ment
• Low standby flow rate prevents precipitation of eluent buf-
Two 6-position 7-port valves integrated into the TCC-3200
fers, increasing instrument uptime.
Thermostatted Column Compartment are used for column
• Application switching can be scheduled for ultimate flex- selection; up to six columns can be scouted automatically. The
ibility. Set method A to run over the weekend, and auto- PDA-3000 Photodiode Array Detector is used for peak purity
matically switch to method B Sunday morning—both will and identity tracking.
be complete when you arrive in the laboratory on Monday.
LPG-3400 TCC-3200 2×6P-7P
Column 1
• Analytical and micro automated application switching kits
include all required tubing and instructions Column 2
Column 3
In this solution, after the operator sets up both LC methods, the
system automatically starts and equilibrates. As the analyses on WPS-3000 1
6
5 1
6
5 DAD-3000
the first column completes, the system automatically switches 2 4 2 4
3 3
to the second method and column without additional opera- Column 4
tor intervention (Figure 5). This approach frees operator time,
increases system use time, and thus boosts productivity. Column 5
Column 6
Smart Startup Application #1 Automated Switchover Application #2 Smart Shutdown
280
Figure 6. Flow scheme for automated method scouting.
80
Quinine
Ca ffeine
Aspartylphenylalanine
WVL: 210 nm WVL: 235 nm
Chromeleon Chromatography Management Software provides

Erythorbic Acid
Dihydroquinine
Benzoic Acid
Cinchonidine
mAU
Aspartame
mAU
easy parameter permutation, without the need for method
changes. The data mining tools, such as automated SST and
–50 –20
0 Minutes 22 0 Minutes 23
23919
query functions, eliminate the need for manual data assessment
and help to quickly identify the optimum set of parameters.
Smart reports visualize results instantaneously.
Figure 5. Automated application switching automates two different
methods in series and minimizes system idle.
The UltiMate 3000 LC system configuration for automated
method scouting includes the SR-3000 Solvent Rack, LPG‑3400
The UltiMate 3000 x2 Dual LC system for automated applica-
Quaternary Pump, TCC-3200 Thermostatted Column Compart-
tion switching includes the SRD-3600 Solvent Rack, DGP-
ment, two 6-position 7-port switching valves, and DAD-3000
3600 Dual-Gradient Pump, WPS-3000SL In-line Split Loop
UV/Vis Detector.
Autosampler, TCC-3200 Thermostatted Column Compartment,
two 2-position 6-port switching valves, and a UV/Vis detector.
This system setup also supports On-Line SPE-LC.
8 www.dionex.com
Proteomics Preconcentration
Proteomics Preconcentration Proteomics Parallel LC
The Proteomics Preconcentration LCi Solution is ideally suited In order to maximize the sample throughput and MS utilization
for the analysis of in-gel digested protein samples. A reversed- when performing nano and capillary LC-MS/MS for peptide

phase trap column is used for sample cleanup and preconcen- sequencing and protein identification, perform peptide precon-
tration of the peptides. The preconcentration configuration has centration and separation on a parallel LC setup. This parallel
the advantage that, by concentrating the sample onto the trap LC configuration includes two preconcentration nano LC setups
column, conventional injection volumes can be made on nano (above) for on-line sample cleanup.
LC columns. In addition, the low flow resistance of the trap
column allows the use of high flow rates to inject large sample • Increased sample throughput for new and existing pro-
volumes in a short time. teomic methods
• Shorter run times due to off-line column regeneration
• Fast injection of large samples in nano LC
• On-line peptide preconcentration, cleanup and separation
• High detection sensitivity
• Parallel LC application kits available for nano, capillary
• On-line cleanup and desalting of samples
and monolithic configurations
• Biocompatibility option
In Proteomics Parallel LC, protein digests are injected onto a
• Preconcentration kits available for nano, capillary, micro trap column for sample cleanup and desalting. After sample
and monolithic configurations cleanup the 2-position valve is switched and the gradient is
• Chromeleon Chromatography Management Software for started to elute the peptides from the trap column. After separa-
easy system and application control tion on the analytical column, the columns are washed and
equilibrated. During this phase, the next sample is injected
The typical workflow in a preconcentration experiment starts onto the second trap and analytical column system to wash and
with sample injection onto a trap column. The sample is precon- separate the peptides. A dedicated nano valve in the Flow Man-
centrated and desalted by flushing an aqueous solvent through ager is used to connect the nano column to the MS detector for
the trap column. After sample cleanup the 2-position valve is peptide identification (Figure 8).
switched to place the trap column in series with the separation
column. The solvent gradient is started to elute and separate the
analytes. The analytes are then detected by UV and/or MS/MS
or are spotted on-line onto a MALDI target (Figure 7).
Figure 8. Flow scheme for proteomics parallel LC.
The parallel LC setup allows simultaneous peptide separation

and column equilibration, for increased sample throughput of up
Figure 7. Flow scheme for proteomics preconcentration. to 100%.
The UltiMate 3000 x2 Nano/Cap LC system for preconcentra- The UltiMate 3000 x2 Nano/Cap LC system for parallel LC
tion and sample cleanup includes the SRD-3600 Solvent Rack, includes the SRD-3600 Solvent Rack, DGP-3600M Micro
DGP-3600M Micro Dual-Gradient Pump, WPS-3000PL Nano/ Dual-Gradient Pump, LPG-3400M Micro Quaternary Pump,
Cap Pulled-Loop Autosampler, FLM-3300 1x2P-10P Flow WPS-3000PL Nano/Cap Pulled-Loop Autosampler, FLM-3300
Manager with one 2-position 10-port switching valve and Flow Manager with one 2-position 10-port switching valve,
VWD-3100 UV/Vis Detector. Standard and biocompatible ver- FLM-3200 Flow Manager with 2-position 10-port and 2-posi-
sions are available. tion 6-port switching valves, and VWD-3100 UV/Vis Detector.
www.dionex.com 9
LCi Solutions
Proteomics MDLC Solutions autosampler supports fully automated off-line 2-D LC, a unique
solution for the analysis of complex proteomics samples.
These 2-D solutions employ the UltiMate 3000 Proteomics
MDLC system to address challenging proteomics applications. The typical workflow for automated off-line MDLC involves
sample injection and separation in the first dimension, collec-

• Combination of two orthogonal chromatographic tech- tion of eluting peaks by the WPS autosampler, re-injection, and
niques, for example, IEX and RP-HPLC subsequent separation in the second dimension. The analytes are
finally detected by UV and MS detection (Figure 10). Off-Line
• Separation of complex samples
2-D LC kits are available for peptides (SCX-RP) and proteins
• High peak capacities (WAX-RP).
• Easy system control and easy visualization of 2-D LC data
with Chromeleon Software
• Simple method development and optimization
• High method flexibility
2-D LC Salt Steps

The 2-D LC salt step solution is the easy-to-use and flexible Figure 10. Flow scheme for off-line 2-D LC.
solution for moderately complex proteomics analyses. It allows
2-D separations by using the autosampler and salt injections to
Comprehensive 2-D LC
transfer fractions from the first to the second dimension.
The comprehensive 2-D LC solution supports the analysis of
The 2-D LC salt step LCi solution is based on injections of salt very complex samples. It allows simultaneous gradient elution
plugs onto an ion-exchange column. Typically, a protein digest of peptides on two separate dimensions. Sample is injected onto
is injected onto a strong cation-exchange column for separation an ion- exchange column and eluted with a salt gradient. Higher
in the first dimension. Peptides are eluted and separated as frac- throughput is achieved by the use of two trap columns; while
tions by injecting salt plugs with increasing salt concentrations. one trap column is loaded with peptides from the SCX, the
Each fraction is subsequently separated on a reversed-phase other is eluted onto the RP nano column with the RP gradient
column in the second, orthogonal dimension (Figure 9). The (Figure 11). Comprehensive 2-D LC kits are available for nano
total number of salt plugs depends on the sample complexity. and capillary systems.
This method can be fully automated. 2-D LC salt step kits are
available for nano, capillary, and monolithic configurations. The
same fluidics also support Proteomics Preconcentration.
Figure 11. Flow scheme for comprehensive 2-D LC.
The UltiMate 3000 Proteomics MDLC system for 2-D LC

Figure 9. Flow scheme for 2-D LC salt steps. Salt Steps and off-line 2-D LC includes the SRD-3600 Solvent
Rack, DGP-3600M Micro Dual-Gradient Pump, WPS-3000PL
Automated Off-Line 2-D LC Nano/Cap Pulled-Loop Autosampler, FLM-3100 Flow Manager
with two 2-position 10-port switching valves, and VWD-3100
The off-line 2-D LC solution supports the separation of highly UV/Vis Detector. The system for comprehensive 2-D LC is
complex proteomic samples. The advantages of off-line MDLC similar, replacing the FLM-3100 with two FLM-3300 Flow
techniques over on-line approaches include high flexibility in Managers, and adding an LPG-3400M Micro Quaternary Pump.
column dimensions and mobile-phase compositions, as well as Standard and biocompatible versions are available.
the ability to re-analyze sample fractions. The micro fraction
collection option of the WPS-3000PL Nano/Cap Pulled Loop
10 www.dionex.com
Rapid Separation LC
Rapid Separation LC
The UltiMate 3000 LCi Solution for Rapid Separation Liq-
uid Chromatography (RSLC) provides the means for ultrafast

separations and high resolution chromatography: Ultrahigh
performance chromatography (UHPLC) at a maximum level of
reliability and flexibility.
• Speed up your analytical LC application by up to a factor

of 50
• Accelerate and revalidate existing LC methods in less than
one week
• Improve resolution for the analysis of complex samples by The Dionex Method Speed-Up Calculator facilitates the process of
increasing peak capacity method adaption to fast LC. Enter your old method details and your
new column details. The calculator immediately provides new method
• Stay flexible with the Rapid Separation LC System in com- parameters and automatically calculates how much time and solvent
bination with Acclaim 1, 2.1, 3 and 4.6 mm ID columns the new method will save.
with particle sizes of 2, 3 and 5 µm
The UltiMate 3000 LCi Solution for RSLC significantly
• Enjoy Chromeleon data processing and reporting tools in- increases sample throughput of LC applications such as amino
cluding 3-D data handling and revalidation tools for instant acid analysis, without compromising resolution.
results.
16 45
9 Arg
The extensive flow-pressure footprint of the UltiMate 3000
3 Asn
1 Asp
11 Tyr
1 Asp
4 Ser
19 Lys
6 His
10 Ala
Rapid Separation LC system and the comprehensive set of Ac-
14 Met
2 Glu
2 Glu
13 Val
5 Gln
8 Thr
15 Trp
7 Gly
16 Phe
19 Lys
3 Asn
claim column formats allow you to exploit the full range of LC 4 Ser
9 Arg
10 Ala
18 Leu
11 Tyr
6 His
21 FMOC Impurity
5 Gln
7 Gly
applications at flow rates up to 5 mL/min and backpressures up

8 Thr
14 Met
21 FMOC Impurity
18 Leu 30.22
13 Val
mAU
15 Trp
16 Phe
mAU
17 Ile
20 Pro-OH
to 800 bars.
22 Pro
17 Ile
12 Cys-Cys
20 Pro-OH
12 Cys-Cys
22 Pro
0
0
800
-6 -5
0 Minutes 41 0 Minutes 6
UltiMate 3000 RSLC Flow-Pressure Footprint 25334 25335
Pressure Range (bar)
Zone 1 Zone 2 Zone 3 Zone 4

RSLC Speed up your amino acid analysis: Conventional reversed-phase LC
Resolution
Speed
Ultrahigh Conventional Very High
High
High
Conventional Very High Ultrahigh
separation of 21amino acids in 41 min (60 min total run time, left
Typical Flow
(mL/min)
0.2–1.5 0.75–2.0 1.0–3.0 2.5–5.0 chromatgram) vs. baseline separation of 21 amino acids in less than
0
0 Column Length (mm) ≥ 100 ≥ 150
≥ 50
≤ 100
≤ 50 6 min (7 min total run time, right chromatogram) on the Acclaim RSLC
5
1
4 Column ID (mm) ≤3 ≥4 ≤3 ≤3 2 μm column.
2 3
Flow Particle Size (µm) ≤3 ≥3 ≤3 ≤3
rate 3 2 ne
(mL/m Zo
in) 4
5
1
The UltiMate 3000 RSLC system configuration for RSLC
analyses includes the SRD-3400 Solvent Rack, HPG-3400RS
With its extensive flow-pressure footprint, RSLC fully meets your
chromatographic goals. Simply work with your column of choice and in Binary Rapid Separation Pump, WPS-3000RS Rapid Separation
the appropriate zone for your application (Zone 1: Ultrahigh resolu- Autosampler, TCC-3000RS Thermostatted Column Compart-
tion/ high speed, Zone 2: Conventional LC, Zone 3: Very high speed/ ment and the DAD-3000RS Diode Array Detector. This setup
very high resolution, Zone 4: Ultrahigh speed/ high resolution). RSLC also supports conventional LC applications.
covers them all.
A method speed-up calculator is provided for quick and easy Note: For more information on the UltiMate 3000 RSLC
conversion of conventional LC to RSLC methods. system and Acclaim columns, please refer to the corresponding
sections of this catalog.
www.dionex.com 11
12
LCi Solutions
www.dionex.com
UltiMate 3000 LC Systems
Flexible, modular systems from nano to semiprep, for
high-productivity Intelligent LC (LCi) solutions

The UltiMate 3000 Intelligent LC (LCi) series for LC and LC/
MS provides ideal solutions for your laboratory. The UltiMate
3000 LCi series provides excellent chromatographic perfor-
mance while maintaining easy and reliable operation. Select
from a wide variety of systems for applications at all scales,
from analytical LC, to Rapid Separation LC for high speed and
high resolution LC, to sensitive nanoflow LC. Basic Systems: UltiMate 3000 Basic LC systems provide reli-
able and high-performing solutions to fit both your bench space
• Superior chromatographic performance and budget.
• Unique x2 dual-gradient option Standard Systems: Choose from a wide variety of Standard
LC systems for demanding LC applications at nano, capillary,
• Decreased run times, even for validated methods
micro, analytical, and semipreparative flow rates.
• Automated off-line multidimensional separations
Rapid Separation LC Systems: The extended flow-pressure
• High instrument utilization times footprint of the RSLC system provides the performance for
ultrafast, high resolution, and conventional LC applications.
• Ease of use
x2 Dual Systems: Learn how UltiMate 3000 x2 Dual Systems
Whichever UltiMate 3000 LC system configuration you choose, and LCi solutions increase productivity and performance while
you will get a highly integrated solution with optimum flu- lightening your task load.
idic connections, single-point and intelligent control through
Chromeleon software, and seamless inter-module communica-
tion.
www.dionex.com 13
Basic Systems Related Literature
For detailed specifications and applications, see the following
PDF documents under Literature on www.dionex.com.
Product Brochures
UltiMate 3000 Basic Automated System
Product Data Sheets
ACC-3000 Autosampler Column Compartment
Basic Manual System
The UltiMate 3000 Basic series matches your current needs and
provides the adaptability you need to meet future challenges.
The fully-scaleable, modular basic system is easily upgraded
as your needs change. UltiMate 3000 basic systems provide the
flexibility, reliability, and performance to make the most of your
equipment investment.
• SmartFlow pulsation-free flows even at high flow rates and

pressures
• High-performance operation for consistent and reliable
results
• Excellent retention time precision, detector sensitivity,
linearity, and drift The UltiMate 3000 Basic Manual System includes a manual
injector and is suitable for classical isocratic or quaternary
• Rugged components to ensure high instrument uptime
gradient LC separations at ambient temperature and analytical
• System Wellness and predictive performance indicators flow rates.
• Modular design for easy module upgrade, addition, or • Excellent retention time and gradient precision due to
replacement SmartFlow technology
• Optional ACC-3000 Autosampler Column Compartment • Manual injection (full- and partial-loop)
for expanded automation
• High detection sensitivity with low detector noise and drift
• Chromeleon Chromatography Management Software for
ease of use and maintenance • Robust, dependable performance at a low cost of ownership
The UltiMate 3000 Basic Automated System integrates straight- The UltiMate 3000 Basic Manual System typically includes the
forward automation using the innovative analytical autosampler ISO-3100A Isocratic Analytical Pump or LPG-3400A Quater-
with integrated column compartment ACC-3000(T), while the nary Analytical Pump. High detection sensitivity is guaranteed
Basic Manual System includes a manual injector for precise by the VWD-3100 Variable Wavelength Detector with data rates
manual injections of your samples. of up to 20 Hz.
Note: For system specifications, please refer to the LC

Modules section.
14 www.dionex.com
Basic Systems
Ordering Information • High detection sensitivity with low detector noise and drift
The following LC modules and accessories represent a typical • Robust, dependable performance at low cost of ownership
UltiMate 3000 Basic Manual system configuration. Due to its
The UltiMate 3000 Basic Automated system is typically con-

modular setup, the system can be upgraded easily by adding or
figured with the ISO-3100A Isocratic Analytical Pump or the
exchanging LC modules. For additional order information and
LPG-3400A Quaternary Analytical Pump. The modular design
key specifications for LC modules and accessories, please refer
supports a variety of configurations, so you can create a Basic
to the LC Modules section.
Automated LC system tailored to your applications.
Hardware Note: For system specifications, please refer to the LC

Modules section.
SR-3000 Solvent Rack (without Degasser).............................................. 5035.9200
ISO-3100A Isocratic Analytical Pump System......................................... 5035.0010
LPG-3400A Quaternary Analytical Pump System.................................... 5035.0015 Ordering Information
Manual Injection Valve Kit, Analytical, UltiMate 3000 Pumps.............. 5035.0600
The following LC modules and accessories represent a typical
VWD-3100 Single Channel Variable Wavelength Detector (without
UltiMate 3000 Basic Automated system configuration. Due to
Flow Cell).................................................................................................. 5074.0005
its modular setup, the system can be upgraded easily by adding
or exchanging LC modules. For additional order information
Accessories and key specifications for LC modules and accessories, please
Syringe, 100 µL, Manual Injectors........................................................... 6035.0665 refer to the LC Modules section.
Flow Cell, Analytical, SST, 11 µL Volume, 10 mm Pathlength,
VWD‑3x00 Series..................................................................................... 6074.0250 Hardware
Basic Automated System ISO-3100A Isocratic Analytical Pump System......................................... 5035.0010
LPG-3400A Quaternary Analytical Pump System.................................... 5035.0015
ACC-3000T Autosampler Column Compartment with Sample
Thermostatting......................................................................................... 5830.0020
Flow Cell).................................................................................................. 5074.0005
Accessories
VWD‑3x00 Series..................................................................................... 6074.0250
The UltiMate 3000 Basic Automated System provides rugged,

automated performance in an economical yet flexible package.
The heart of this system is the ACC-3000 Autosampler Column
Compartment, a combined autosampler and column compart-
ment, for cost-conscious automation and the best available value
for your budget.
• Excellent retention time and gradient precision due to

SmartFlow technology
• Pulled-loop injection principle (full- and partial-loop injec-
tions)
• Fast and stable column thermostatting between 5 °C above
ambient and 50 °C
www.dionex.com 15
Standard Systems Product Data Sheets
UltiMate 3000 Titanium System Data Sheet
Binary Analytical System

The UltiMate 3000 Binary Analytical system has been carefully

configured to meet the demands of analytical LC analyses at
pressures up to 500 bar. Two serial dual-piston pumps provide
stable, accurate pulse-free flows—essential for optimal binary
gradient mixing performance—for consistent gradient forma-
tion, rapid gradient response, and excellent retention time
precision.
• SmartFlow technology for precise and accurate flow rates

0.1 to 10 mL/min
• High-pressure gradient proportioning at superior accuracy
and precision
UltiMate 3000 Standard LC systems provide the right solu-
• Minimized gradient delay volume for rapid gradient re-
tion for demanding microbore, analytical, and semipreparative
sponse
LC applications. System components are perfectly matched to
meet requirements such as low extracolumn and gradient delay • High temperature operation for lower viscosity
volume for high separation efficiency and low gradient response
times, as well as superior mixing performance. • Ultralow carryover, high precision auto-injections
• Also suitable for fast LC applications (in combination with
• SmartFlow technology for pulsation free flows even at high
the RSLC conversion kit)
flow rates and pressures
• UltiFlow technology for nano/cap/micro flow rates (down The UltiMate 3000 Binary Analytical system provides fast cycle
to 50 nL/min) time, reliable mobile phase temperature conditioning, and high
data rate detection to ensure optimal performance for the full
• Superior binary high-pressure or quaternary low-pressure range of binary analytical applications. In combination with
gradient proportioning the RSLC conversion kit it also facilitates LC applications on
narrow-bore columns with sub-2 to 3 µm particles.
• Active rearseal wash for increased piston seal lifetime
• Patented pistonseal tightness monitoring Note: For system specifications, please refer to the
LC Modules section.
• Variable mixing volumes for optimal mixing performance
• High-precision sampling from multiple formats (wellplates, Ordering Information
vials)
The following modules and accessories represent a typical
• System Wellness and predictive performance indicators
UltiMate 3000 Binary Analytical system configuration. Due to
• Biocompatible option for reliable and robust bioanalytical its modular setup, the system can be upgraded easily by adding
LC applications or exchanging LC modules. For additional order information
and key specifications for LC modules and accessories, please
Choose from a wide variety of Standard LC system configura- refer to the LC Modules section.
tions, or modify system setups to fit your specific demands and
applications.
Hardware
SRD-3200 Solvent Rack with 2 Degasser Channels............................... 5035.9250
Related Literature
HPG-3200A Binary Analytical Pump System........................................... 5035.0016
For detailed specifications and applications, see the following WPS-3000TSL Analytical In-Line Split-Loop Thermostatted
PDF documents under Literature on www.dionex.com. Autosampler............................................................................................. 5822.0020
TCC-3000SD Thermostatted Column Compartment............................... 5730.0010
Product Brochures
MWD-3000 Multiple Wavelength Detector (without Flow Cell)........... 5082.0030
Ultimate 3000 System Brochure
UltiMate 3000 Nano and Capillary LC System
16 www.dionex.com
Standard Systems
Accessories Hardware
Flow Cell, Analytical, SST, 13 µL Volume, 10 mm Pathlength, SR-3000 Solvent Rack (without Degasser).............................................. 5035.9200
DAD‑3000 and MWD-3000 Series.......................................................... 6082.0100 LPG-3400A Quaternary Analytical Pump System.................................... 5035.0015
Note: For more information on the RSLC conversion kit, please

WPS-3000TSL Analytical In-Line Split-Loop Thermostatted
refer to the LCi Solution Kits section Autosampler............................................................................................. 5822.0020
Quaternary Analytical System DAD-3000 Diode Array Detector (without Flow Cell)............................. 5082.0010
The UltiMate 3000 Quaternary Analytical system offers techni-
cal and practical benefits to almost any laboratory and applica- Accessories
tion area. It supports the widest range of solvent options and
gradient profiles, making method development easy. DAD‑3000 and MWD-3000 Series.......................................................... 6082.0100
Note: For more information on the RSLC conversion kit, please
• SmartFlow technology for precise and accurate flow rates refer to the LCi Solution Kits section
from 0.2 to 10 mL/min
• Quaternary low-pressure proportioning at superior accuracy
Biocompatible Analytical System
and precision
• Convenient solvent selection for eluent preparation and The UltiMate 3000 Titanium system is a fully biocompatible
system flushing analytical LC designed for high-performance analysis and pu-
rification of proteins. The wide range of solvent options allows
• Multiple choices and combinations of gradient profiles easy implementation of different gradient profiles, essential for
(linear, step, concave, convex) method development.
• Ultralow carryover, high precision auto-injections
• Titanium pumps and PEEK flow-path for full biocompat-
• Wide temperature range, thermostatting of samples and ibility
columns
• Diode Array Detector for convenient method development from 0.2 to 10 mL/min
at high detection sensitivity
• Low-pressure proportioning at superior accuracy and preci-
• Biocompatible version available (UltiMate 3000 Titanium sion
system)
• Convenient solvent selection for eluent preparation and
system flushing
The UltiMate 3000 Quaternary Analytical system provides
remarkable retention time precision, accurate and precise eluent • Ultralow carryover, high precision auto-injections
selection and proportioning efficiency, and excellent mixing per-
formance for quaternary analytical applications. In combination Titanium pumps and an all-PEEK flow-path on the UltiMate
with the RSLC conversion kit it also facilitates LC applications 3000 Titanium system ensure full biocompatibility, critical to
on narrow-bore columns with sub-2 to 3 µm particles. the integrity of proteins during separation, while delivering high
day-to-day reproducibility and robust operation even under
Note: For system specifications, please refer to the harsh salt and pH conditions.
LC Modules section.
Note: For system specifications, please refer to the
Ordering Information LC Modules section.

UltiMate 3000 Quaternary Analytical system configuration. Ordering Information
Due to its modular setup, the system can be upgraded easily by
adding or exchanging LC modules. For additional order infor-
UltiMate 3000 Titanium system configuration. Due to its
mation and key specifications for LC modules and accessories,
modular setup, the system can be upgraded easily by adding or
please refer to the LC Modules section.
exchanging LC modules. For additional order information and
www.dionex.com 17
Hardware Hardware
SR-3000 Solvent Rack (without Degasser).............................................. 5035.9200 SR-3000 Solvent Rack (without Degasser).............................................. 5035.9200
LPG-3400AB Biocompatible Quaternary Analytical Pump System......... 5037.0015 LPG-3400M Quaternary Micro Pump System......................................... 5035.0045
WPS-3000TBPL Analytical Biocompatible Pulled-Loop Thermostatted WPS-3000TRS Rapid Separation Thermostatted Autosampler............. 5840.0020
Autosampler............................................................................................. 5823.0020 TCC-3000SD Thermostatted Column Compartment............................... 5730.0010
TCC-3000SD Thermostatted Column Compartment............................... 5730.0010 VWD-3400RS Rapid Separation Variable Wavelength Detector........... 5074.0010
VWD-3400RS Rapid Separation Variable Wavelength Detector........... 5074.0010
Accessories
Accessories
Flow Cell, Semi-Micro, SST, 2.5 µL Volume, 7 mm Pathlength,
Flow Cell, Analytical, PEEK, 11 µL Volume, 10 mm Pathlength, VWD‑3x00 Series..................................................................................... 6074.0360
VWD‑3x00 Series..................................................................................... 6074.0200
Sample Loop, 25 µL, WPS-3000RS and WPS-3000SL Analytical
Samplers................................................................................................... 6820.2415
Quaternary Micro System
The UltiMate 3000 Quaternary Micro system is the ideal choice Binary Semipreparative System
for laboratories that need flexibility for microbore LC and LC/
MS analyses. The selection of up to four solvents and quaterna- The UltiMate 3000 Binary Semipreparative system is tailored
ry gradient profiles accelerates method development and offers for robust, safe, and high-performance purifications. Based on
flexibility during automated system startup and shutdown. proven high-pressure gradient principles, the system takes full
advantage of UltiMate 3000 features such as SmartFlow pulse-
• SmartFlow technology for precise and accurate flow rates free eluent delivery and wide linear range detection.
from 10 to 2500 µL/min
• Wide operating flow rate range from 0.5 to 50 mL/min
• Quaternary low-pressure proportioning at superior accuracy
and precision • SmartFlow technology for precise and accurate flow rates
• Convenient solvent selection for eluent preparation and • High-pressure proportioning at high accuracy and precision
system flushing • Variable mixing volumes for different flow and delay vol-
• Selection of choices and combinations of gradient profiles ume requirements
(linear, step, concave, convex) • Sample injections up to 2.5 mL with excellent volumetric
• Ultralow carryover, high precision µL auto-injections linearity
• Wide temperature range, thermostatted control of samples • Chromeleon solvent and waste level monitor
and columns
The UltiMate 3000 Binary Semipreparative system supports
• Wide selection of high-performance detectors high flow rates at low backpressure, thanks to the superior flu-
idic design of the semipreparative autosampler. The system is a
The UltiMate 3000 Quaternary Micro system combines lowest robust platform for your purification of natural product extracts,
detection limits, reduced baseline noise, and maximum flexibil- isolation of impurities for structural elucidation, or other large-
ity. It is recommended as a micro LC front-end for LC/MS plat- scale LC processes.
forms and other micro LC applications. With its reduced system
delay volume and high detection data rates, it is also suitable for Note: For system specifications, please refer to the
fast and very fast microbore LC applications. LC Modules section.
Note: For system specifications, please refer to the

LC Modules section. Ordering Information

Ordering Information
UltiMate 3000 Binary Semipreparative system configuration.
The following modules and accessories represent a typical Ul- Due to its modular setup, the system can be upgraded easily by
tiMate 3000 Quaternary Micro system configuration. Due to its adding or exchanging LC modules. For additional order infor-
modular setup, the system can be upgraded easily by adding or mation and key specifications for LC modules and accessories,
exchanging LC modules. For additional order information and please refer to the LC Modules section.
18 www.dionex.com
Standard Systems
Hardware The UltiMate 3000 Nano/Cap/Micro Quaternary system
features a unique Flow Manager. Its active flow splitter is
HPG-3200P Binary Semipreparative Pump System................................ 5035.0025
responsible for nano/cap/micro flow delivery while column
WPS-3000TSL Semipreparative In-Line Split-Loop Thermostatted
thermostatting ensures superior retention time reproducibility.
Autosampler............................................................................................. 5822.0028

The system’s UV detector with dedicated low volume nano/cap/
VWD-3100 Single Channel Variable Wavelength Detector, without micro flow cells is ideal to monitor separation results prior to
flow cell.................................................................................................... 5074.0005
MS detection.
Accessories Note: For system specifications, please refer to the

Flow Cell, Semipreparative, PEEK, 0.7 µL Volume, 0.4 mm Pathlength, LC Modules section.
VWD-3x00 Series..................................................................................... 6074.0320
Quaternary Nano/Cap/Micro System
The following modules represent a typical biocompatible Ulti-
Mate 3000 Quaternary Nano/Cap/Micro system configuration.
Due to its modular setup, the system can be upgraded easily by
adding or exchanging LC modules. For additional order infor-
mation and key specifications for LC modules and accessories,
please refer to the LC Modules section.
Hardware
LPG-3400MB Biocompatible Quaternary Micro Pump System.............. 5037.0055
WPS-3000TBPL Nano/Cap Biocompatible Pulled-Loop Thermosttated
Autosampler............................................................................................. 5821.0020
FLM-3300B, Nano, 1X2P-10P Biocompatible Flow Manager with
2-Position 10-Port Switching Valve......................................................... 5721.0030
Flow Cell).................................................................................................. 5074.0005
The UltiMate 3000 Quaternary Nano/Cap/Micro system of-
fers precise, low flow rates down to 50 nL/min, and ultrahigh
sensitivity. It is the perfect fit when working with limited sample Accessories
volumes or performing low-flow LC/MS applications. In its UZ-View Flow Cell, Nano, 3 nL Volume, 10 mm Pathlength, VWD‑3x00
standard configuration it allows the reliable direct injection and Series........................................................................................................ 6074.0270
1D-separation of a minimum of 20 nL out of a 1 µL sample.
• UltiFlow technology for precise and accurate flow rates

down to 50 nL/min
• High-precision sampling from well plates and microvials
• Exact temperature control for all critical flow path compo-
nents protects thermolabile samples
• Reliable injection reproducibility, down to 20 nL
• Excellent front end for MS detectors
• System Wellness and predictive performance indicators
• Seamless MS integration with single-point software control
for most MS platforms
www.dionex.com 19
Rapid Separation LC Systems
Ultimate Speed, Ultimate Resolution, Ultimate
Performance
The UltiMate 3000 Rapid Separation LC (RSLC) system

integrates unique hardware features for ultrafast separations and
excellent resolution on columns with small particles. In addi-
tion, it is characterized by a maximum level of reliability and
flexibility.
• SmartFlow pulsation-free flows at flow rates up to 5 mL/

min at pressures up to 800 bar (11,600 psi)
• High pressure gradient proportioning at superior accuracy
and precision
The Dionex UltiMate 3000 Rapid Separation LC (RSLC) system
• Easily adjustable system gradient delay volume supporting
a wide range of LC applications
• In-line split-loop injections for 15 second, no-sample-loss
injections
• Column compartment temperatures up to 110 °C for re-
duced system backpressure
• Data collection rates up to 100 Hz with variable wave-
length, multiple wavelength, and diode array detection
• UV spectra data of even the sharpest peaks for peak purity
analysis (diode array detection)
• Run both ultrafast separations and conventional LC
methods
The UltiMate 3000 RSLC system allows you to work over an Separation of 10 compounds in 10 seconds, with a full 100 Hz DAD
extensive flow-pressure footprint up to 5 mL/min and 800 bar. spectral scan. Flow rate: 3.7 mL/min, backpressure: 730 bar, oven
This covers the full range of HPLC applications on one system, temperature: 100 °C.
including conventional and ultrafast LC, on one system.
The Rapid Separation Autosampler allows robust 100 µL injec- Ultimate Speed and Performance
tions over the full backpressure range. In addition, it handles a
The Ultimate 3000 RSLC system in combination with the
multitude of different sample formats and supports a maximum
UltiMate 3000 Rapid Separation Diode Array Detector supports
of 1167 samples. The column compartment can be upgraded
peak characterization of the sharpest peaks of ultrafast separa-
easily and converted with different column switching valves.
tions, for reliable sample data. No matter if you run RSLC or
The compartment handles up to 12 columns (depending on
conventional LC methods, the UltiMate 3000 RSLC system will
column dimensions) up to 30 cm length. UV detectors come
convince you with its ultimate performance.
with a wide variety of different flow cells.
Do you need shorter run times for high sample throughput or in-
stant results in process analyses? Does your current LC method
lack resolution, and do you want to generate higher peak capac-
ity? Then learn more about the benefits which are provided by
the RSLC system in combination with Acclaim RSLC columns.
Refer to the corresponding LCi Solution section in this catalog.
20 www.dionex.com
Rapid Separation LC Systems
RT RSD: 0.04%
Area RSD:
Area RSD: 0.11%
RT RSD:
RT RSD: 0.04%
Area RSD:
A
0.04%
0.12%
700
Area RSD: 0.12%

Column specifications:
0.11%
RT RSD: 0.04%
250 x 4.6 mm id The following RSLC modules and accessories configure the
5 µm particles
most flexible RSLC system. For additional RSLC modules and

mAU
Excellent retention time and area accessories, order information, and key specifications, please
precision in conventional mode
(10 injections) refer to the LC Module section of this catalog.
0
0 Minutes 12
Area RSD:
Area RSD: 0.28% Hardware
RT RSD:
B RT RSD: 0.06%
0.03%
0.22%
Area RSD:
Area RSD: 0.23%

300 Column specifications:
RT RSD:
RT RSD: 0.03%
0.04%
0.30%
75 x 3 mm id
3 µm particles
HPG-3400RS Binary Rapid Separation Pump System with Solvent
mAU
Excellent retention time and

area precision in UHPLC Selector Valves......................................................................................... 5040.0046
mode (10 injections)
WPS-3000TRS Rapid Separation Thermostatted Autosampler, ........... 5840.0020
0
0 Seconds 90 25337
TCC-3000RS Rapid Separation Thermostatted Column Compartment.. 5730.0000
The RSLC system provides excellent retention time precision for both DAD-3000RS Rapid Separation Diode Array Detector (without Flow
conventional LC (A) and UHPLC (B) separations (gradient applica- Cell)........................................................................................................... 5082.0020
tion).
Accessories
Related Literature Static Mixer Kit, 200 µL, HPG3x00RS Pumps.......................................... 6040.5150
Sample Loop, 2.5 µL, WPS-3000RS and WPS-3000SL Analytical
For detailed specifications and applications, see the following Samplers................................................................................................... 6820.2415
PDF documents under Literature on www.dionex.com. Flow Cell, Analytical, SST, 13 µL Volume, 10 mm Pathlength,
DAD‑3000 and MWD-3000 Series.......................................................... 6082.0100
Product Brochures Flow Cell, Semi-Micro, SST, 2.5 µL Volume, 7 mm Pathlength,
UltiMate 3000 RSLC System Brochure
Product Data Sheets
UltiMate 3000 Rapid Separation LC (RSLC) System Data Sheet
www.dionex.com 21
x2 Dual Systems Related Literature
Product Brochures
Ultimate 3000 System Brochure
UltiMate 3000 Proteomics MDLC System Brochure
Product Datasheets
UltiMate 3000 Proteomics MDLC System Brochure
x2 Dual Analytical System

The UltiMate 3000 x2 Dual Analytical system is the ideal
No matter the flow rate range—nano, capillary, micro or analyti- choice to maximize sample throughput for your analytical LC
cal—UltiMate 3000 x2 Dual systems increase productivity with separations. The system combines the flexibility of ternary sol-
Intelligent LC (LCi) solutions for parallel and tandem chro- vent selection with the productivity and performance of x2 Dual
matography, on-line sample preparation or multi-dimensional LC. The modular design is easily customized to your applica-
chromatography (MDLC). tions and sample preparation needs.
• SmartFlow technology for pulsation-free flows • SmartFlow technology for precise and accurate flow rates
from 0.2 to 10 mL/min
• UltiFlow technology for ultra-reproducible nano/cap/micro
flow rates down to 50 nL/min • Optimized extracolumn volume for superior separation
efficiency
• Superior dual low-pressure gradient proportioning
• Ultralow carryover, high precision µL auto-injections
• The throughput of two LC systems in the footprint of
one LC • Wide temperature range, thermostatted control of samples
and columns
• Active rear-seal wash for increased piston seal lifetime
• UltiMate 3000 x2 Dual Titanium system available
• High-precision sampling from multiple formats (wellplates,
vials) Intelligent x2 Dual LC solutions save time and effort. Parallel
• System Wellness and predictive performance indicators and tandem configurations increase sample throughput by up
to 100%, by automating two applications in one “run.” Multi-
• Biocompatible option for robust and reliable bioanalytical dimensional separations provide resolution impossible with
LC applications single-column methods. On-line sample preparation saves time
and reduces complexity, sample loss, and matrix interferences.
UltiMate 3000 x2 Dual systems feature SmartFlow and Ulti-
Flow technology for pulse-free eluent delivery and accurate low Note: For system specifications, please refer to the
flow rates, as well as a wide linear detection range. Chromeleon LC Modules section.
software fully supports validation and documentation of all
x2 dual configurations, while Chromeleon Wizards simplify
method implementation. Ordering Information
Note: For further informations on LC applications utilizing The following LC modules represent a typical x2 Dual Ana-
x2 Dual systems, please also refer to the LCi Solutions section. lytical system configuration for tandem analyses. For order
information on LC modules and key specifications, see the LC
Modules section. See also “LCi Solutions” for more about par-
allel and tandem LC, 2-D LC, on-line sample preparation, and
application switching.
22 www.dionex.com
x2 Dual Systems
Hardware Ordering Information
DGP-3600A Dual-Gradient Analytical Pump System.............................. 5035.0014 UltiMate 3000 x2 Dual Titanium system configuration. For

WPS-3000TRS Rapid Separation Thermostatted Autosampler............. 5840.0020 order information on LC modules and key specifications, see the
TCC-3200 Thermostatted Column Compartment with Two Built-In LC Modules section.
Valve Drives (without Valve Pods)........................................................... 5722.0035
DAD-3000 Diode Array Detector (without Flow Cell)............................. 5082.0010
Hardware
Accessories SRD-3600 Solvent Rack with 6 Degasser Channels............................... 5035.9230
DGP-3600AB Biocompatible Dual-Gradient Analytical Pump System... 5037.0014
2-Position 10-Port Valve Pod, <345 bar (5000 psi), TCC-3x00 Series..... 6722.9023
Two valve pods are required. WPS-3000TBPL Analytical Biocompatible Pulled-Loop Thermostatted
Autosampler............................................................................................. 5823.0020
DAD‑3000 and MWD-3000 Series.......................................................... 6082.0100 TCC-3000SD Thermostatted Column Compartment............................... 5730.0010
Tandem Operation Tubing Kit for Analytical Dual-Ternary UltiMate VWD-3400RS Rapid Separation Variable Wavelength Detector........... 5074.0010
3000 Systems........................................................................................... 6037.0001
Accessories
Biocompatible x2 Dual Analytical Valve Actuation Kit, Right Side, <413 bar (6000 psi), TCC-3000RS and
TCC-3000SD Series.................................................................................. 6730.0003
System Valve Actuation Kit, Left Side, <413 bar (6000 psi), TCC-3000RS and
TCC-3000SD Series.................................................................................. 6730.0004
The UltiMate 3000 x2 Dual Titanium system is a fully biocom-
2-Position 10-Port Valve Pod, Biocompatible, PEEK, <345 bar (5000
patible analytical LC system for advanced multidimensional
psi), TCC-3x00 Series............................................................................... 6723.9023
(MD) protein analysis and purification. It supports advanced
Two valve pods are required.
biochromatography schemes such as application switching
and automated MD chromatography, for example, purification Flow Cell, Analytical, PEEK, 11 µL Volume, 10 mm Pathlength, VWD-
and analysis of biotherapeutics like monoclonal antibodies in a 3x00 Series............................................................................................... 6074.0200
single method.
• Titanium pumps and PEEK flow path for full

x2 Dual Micro System
biocompatibility
The UltiMate 3000 x2 Dual Micro system is an ideal choice
• SmartFlow technology for precise and accurate flow rates to maximize sample throughput of microbore LC applications.
from 0.2 to 10 mL/min It combines the flexibility of ternary solvent selection with the
productivity and performance of x2 Dual LC. In addition, the
• Low-pressure proportioning at superior accuracy and modular design enables easy customization to your applications
precision and preferences.
• Dual ternary pumps for true parallel, tandem or multi-
dimensional chromatography
from 10 to 2500 µL/min
system flushing
system flushing
• Ultralow carryover, high precision auto-injections
• Microbore optimized system gradient delay volume
• Chromeleon CMS—powerful, easy-to-use and provides
• Ultralow carryover, high precision µL auto-injections
tools for 21 CFR Part 11 compliance
• Wide temperature range, thermostatted control of samples
Titanium pumps and PEEK flow paths make the UltiMate and columns
3000 Titanium system fully biocompatible, ensuring biological
integrity of proteins during separation. It features dual gradient Intelligent LC (LCi) solutions with the x2 Dual Micro system
pumps in a single module to support parallel, tandem, and mul- boost productivity and lighten your workload. Parallel and
tidimensional separations for highest flexibility and productivity tandem configurations increase sample throughput of microbore
while saving money and space. separations by up to 100%. On-line sample preparation reduces
matrix interferences and speeds analysis. For LC/MS applications,
Note: For system specifications, please refer to the this means better productivity, performance, and detection limits.
LC Modules section. Note: For system specifications, please refer to the
LC Modules section.
www.dionex.com 23
Ordering Information • Wide flow rate range: 50 nL/min–2.5 mL/min
The following LC modules and accessories represent a typical • Dual-gradient pump with two independent ternary gradient
x2 Dual Micro System configuration for tandem analyses. For pumps with superior gradient accuracy and precision
order information on LC modules and accessories needed for • Easily convertable between nano, capillary and micro flow
parallel analyses, 2-D LC, on-line sample preparation, and ap- rates
plication switching, please refer to the LCi Solutions section of
this catalogue. • UltiFlow technology for constant flow independent of elu-
ent composition and backpressure
Hardware • Low volume nano/cap/micro flow cells for UV/Vis detector
SRD-3600 Solvent Rack with 6 Degasser Channels............................... 5035.9230 • Biocompatibility option

DGP-3600M Dual-Gradient Micro Pump System.................................... 5035.0050 • Micro fraction collection option for fully automated off-line
WPS-3000TSL Analytical In-Line Split-Loop Thermostatted MDLC
Autosampler............................................................................................. 5822.0020
• Supports key solutions such as automated off-line 2-D LC
TCC-3200 Thermostatted Column Compartment with Two Valve
Drives (without Valve Pods)..................................................................... 5722.0035 and 2-D LC using salt plugs
VWD-3400RS Rapid Separation Variable Wavelength Detector........... 5074.0010
The UltiMate 3000 Proteomics MDLC system supports orthog-
onal separation techniques such as ion-exchange and reversed-
Accessories phase chromatography in on-line and off-line 2-D LC mode. It
Sample Loop, 25 µL, WPS-3000RS and WPS-3000SL Analytical is the ideal LC system for bioanalytical laboratories that want to
Samplers................................................................................................... 6820.2415 profit from automated sample preparation and higher sensitivity.
2-Position 10-Port Valve Pod, <345 bar (5000 psi), TCC-3x00 Series..... 6722.9023
Two valve pods are required. Note: For system specifications, please refer to the LC
Modules section.
VWD‑3x00 Series..................................................................................... 6074.0360
Tandem Operation Tubing Kit for Micro Dual-Ternary UltiMate 3000 Ordering Information
Systems.................................................................................................... 6037.0002
Ultimate 3000 Proteomics MDLC system configuration. It is
x2 Dual Nano/Cap/Micro System
the most flexible system for preconcentration and proteom-
ics MDLC solutions such as automated off-line 2-D LC and
2-D LC salt plug. For more information on these LCi solutions
as well as comprehensive information about 2-D LC and paral-
lel LC, please refer to the LCi Solutions section.
Hardware
DGP-3600MB Biocompatible Dual-Gradient Micro Pump System......... 5037.0060
WPS-3000TBPL Nano/Cap Biocompatible Pulled-Loop Thermostatted
Autosampler............................................................................................. 5821.0020
Automated Off-Line 2-D LC Solution, Biocompatible, WPS-3000PL...... 6821.0050
FLM-3100B, Nano, 2X2P-10P Biocompatible Flow Manager with Two
2-Position 10-Port Switching Valves........................................................ 5721.0010
Flow Cell).................................................................................................. 5074.0005
The UltiMate 3000 Proteomics MDLC system, which is based
on the x2 Dual Nano/Cap/Micro system, is the essential tool for
multidimensional LC separations of highly complex biological Accessories
samples. The Dual-Gradient Nano/Cap/Micro Pump provides UZ-View Flow Cell, Nano, 3 nL Volume, 10 mm Pathlength, VWD‑3x00
two independent, precise flow rates; the UltiFlow eluent deliv- Series........................................................................................................ 6074.0270
ery system allows flow rates down to 50 nL/min. The system is
highly flexible, supporting a wide range of MDLC applications.
24 www.dionex.com
LC Modules
The right LC modules for your application needs

UltiMate 3000 LC modules integrate innovation and intelligent
features into a broad selection of autosampler, injector, pump,
flow-control, thermostatted column compartment, and detector
modules. Our quality designs and construction ensure reliable,
precise, and accurate operation. Select the right modules to
match your LC application, whether it requires a nano, capillary,
microbore, analytical, or semipreparative flow configuration.
Solvent Tray/Degasser: For high efficiency degassing and safe
• Superior chromatographic performance solvent bottle organization a Dionex Solvent Rack is the ideal
• Unique x2 dual-gradient configurations complement to every UltiMate pump.
• Unsurpassed instrument reliability LC Pumps: Dionex offers a variety of LC pumps and provides
top solutions from nano to semipreparative scale and ultrahigh
• Ease of use through powerful Chromeleon software performance applications (UHPLC).
• Intelligent LC (LCi) solutions for unparalleled productivity LC Injectors & Autosamplers: Select the right Dionex LC
autosampler module or manual injector to match your LC nano,
Each UltiMate 3000 LC module provides a highly integrated capillary, micro, analytical, or semipreparative system needs.
solution with optimum fluidic connections, single-point control
through Chromeleon software, and seamless intermodule Flow Managers: The Flow Manager is the key component for
communications. chromatography users working at nano/cap/micro flow rates and
requiring high retention time precisions.
LC Column Compartments: For optimal retention time preci-
sion at different temperatures, a Dionex Thermostatted Column
Compartment is essential for your LC system.
LC Detectors: A wide selection of LC detectors to meet the
selectivity and sensitivity needs of your LC applications.
Fraction Collection: Offering fraction collection options for LC
applications at different flows, Dionex provides the right solu-
tion for your fraction collection needs.
www.dionex.com 25
LC Modules
LC Solvent Tray/Degasser • Securely organizes up to nine 1 L eluent bottles on top of
your UltiMate 3000 system tower
• Conserves valuable bench space
• Offers optimum resistance to the most commonly used

HPLC solvents and buffer solutions
Key Specifications
Solvent Reservoir Capacity: nine 1 L reservoirs, or four 2.5 L
reservoirs, or two 5 L reservoirs and two 1 L reservoirs
Degassing Channels: none
Wetted Parts (Tubing and Inline Filter): PEEK, FEP, and Tefzel;
Inline Filter Frit: SST or titanium
Dimensions (h × w × d): 10 × 42 × 51 cm (3.9 × 16.5 × 20 in.)
These online, chemically inert degassers provide unsurpassed

performance and ensure stable baselines free of air bubble-
related disturbances. From the two-degasser rack for a binary
pump to the most flexible six-degasser rack for the powerful Hardware
dual-ternary pump, the UltiMate 3000 solvent rack series has a SR-3000 Solvent Rack (without Degasser).............................................. 5035.9200
solvent rack to fit your system.
• Teflon AF metal-free membranes Solvent Racks with Degasser

• Highest degassing efficiency and fastest degassing system
The UltiMate 3000 Solvent Racks SRD-3200, SRD-3400, and
equilibration times
SRD-3600 incorporate a low-volume (670 µL), chemically-inert
• Neatly stacks onto the UltiMate 3000 LC system tower, degasser with two, four, or six channels. They support pumps
conserving bench space without built-in degassers, such as the Dual-Gradient Analytical
Pump with six channels, and high-pressure gradient pumps with
• User-friendly design with three LEDs (power, vacuum, and two or four channels.
leak sensor status)
• Continuous vacuum level monitoring via UltiMate 3000 • Highest degassing efficiency, ensures stable baseline
System Wellness functionality • Securely organizes up to nine 1 L eluent bottles on top of
• Easy connection to all fluidic lines your UltiMate 3000 system tower
• Conserves valuable bench space
Related Literature • Optimum resistance to the most commonly used HPLC

solvents and buffer solutions
PDF documents under Literature on www.dionex.com. Key Specifications
Solvent Reservoir Capacity: nine 1 L reservoirs, or four 2.5 L
Product Data Sheets
reservoirs, or two 5 L reservoirs and two 1 L reservoirs
UltiMate 3000 Solvent Racks Degassing Channels: two, four, or six analytical degasser
channels
Solvent Rack without Degasser Degasser Membranes: Teflon AF
The UtliMate 3000 Solvent Rack SR-3000 without degasser Channel Volume: 670 µL
supports pumps with integrated degassers as a solvent organizer,
Maximum Flow Rate per Channel: 14 mL/min
and is therefore recommended in combination with UltiMate
3000 Quaternary Analytical and Micro Pumps with internal Control: controlled by the UltiMate 3000 system pump (with
degassers (LPG-3400A/AB/M/MB). Chromeleon) or in standalone operation
26 www.dionex.com
LC Solvent Tray/Degasser
Communication: 15-pin D-Sub connector (through a pump of
the UltiMate 3000 system
Power Supply: 15-pin D-Sub connector (through a pump of the
UltiMate 3000 system) or external power supply unit

User Input/Display: three LEDs (Power, Vacuum, and Status),
standby button
Wetted Parts (Incl. Tubing and Inline Filter): Teflon AF, PEEK,
FEP, and Tefzel; Inline Filter Frit: SST or titanium
Power Requirements: max. 30 VA
Hardware
Accessories
Power supply unit 24V for SOR-100 and SRD-3x00................................ 6510.0004
For use with other than UltiMate 3000 pumps
www.dionex.com 27
LC Modules
LC Pumps • Patented piston-seal tightness monitoring, self-diagnostics,
and pre-programmed test routines
• Minimized gradient delay volumes as low as 35 µL in com-
bination with SmartFlow technology
• Easy front-panel access to all fluidic components for rou-

tine maintenance
• Unique dual-gradient pumps for ultimate flexibility without
occupying additional bench space
• Ultra-precise tachometer-controlled DC motors with near-
zero acoustic noise
For nano, capillary and low micro scale separations (biocompat-

ible) Quaternary Micro and Dual-Gradient Micro Pumps are
combined with the patented nano, capillary, and micro UltiFlow
eluent delivery systems, which are delivered with the corre-
sponding flow managers.
Whether your challenges are high resolution nanoflow or fast
microflow separations, precise QC analyses, or semipreparative
purification applications, the UltiMate 3000 pump family offers
the right solution. All UltiMate 3000 pump modules provide Related Literature
best value in performance, reliability, and ease of use.
• High quality components with intelligent mechanical and PDF documents under Literature on www.dionex.com.
electronic design for high reliability
Product Data Sheets
• Unique SmartFlow technology with automatic compensa-
tion for changing eluent compressibility UltiMate 3000 Pumps Datasheet
• Virtually pulse-free eluent delivery, without pulse damper UltiMate 3000 Rapid Separation LC (RSLC) System Data Sheet
• Sapphire floating piston design—no piston seal distortion
for longer seal lifetime
• Automated active rear-seal flushing for increased seal life
28 www.dionex.com
LC Pumps
RSLC Binary Pump Key Specifications
Recommended Flow Rate Range (Settable Range): 0.100–
5.000 mL/min (0.001–5.000 mL/min)

Flow Rate Accuracy: ±0.1% at 1 mL/min and 20 MPa
Flow Rate Precision: <0.1% RSD at 1 mL/min and 20 MPa
Pressure Range: 2–80 MPa (11,600 psi)
Pressure Ripple: Typically <2 bar or <1%, whichever is greater
Proportioning Accuracy: ±0.2% at 2 mL/min at 40 MPa
Proportioning Precision: <0.2% SD at 2 mL/min at 40 MPa
Gradient Delay Volume: 400 µL (35, 100, or 200 µL with
optional mixer kits)
PC Connection: all functions controllable via USB 2.0; inte-
grated USB hub with three USB 2.0 ports
The UltiMate 3000 Binary Rapid Separation Pump HPG-
3x00RS is designed to operate at ultrahigh backpressures and I/O Interfaces: two digital inputs; two relay outputs
high flow rates simultaneously. Achieve the highest separation
efficiency in combination with long, small bore ≤ 2 µm columns User Input/Display: LCD indicating system parameters,
or use with conventional LC columns: The RS pump is the most Standby button, three LEDs for status monitoring, four function
flexible UHPLC pump available. keys for initial operation and maintenance
GLP Features: Automatic Instrument Qualification (AutoQ),
• Serial dual-piston operating principle, high-pressure gradi- System Wellness, and Qualification Monitoring with
ent proportioning Chromeleon
• Wide operating flow rate range from 100 µL/min to 5 mL/ Power Requirements: 100–120 V, 60 Hz; 200–240 V, 50 Hz
min with high flow rate accuracy
• Support of operating pressures of up to 800 bar (11,600 psi)
• SmartFlow technology for high flow rate, gradient, and Ordering Information
retention time precision
• Perfectly balance gradient delay volume against mobile Hardware
phase mixing efficiency HPG-3200RS Binary Rapid Separation Pump System............................. 5040.0026
• Optional solvent selection valves support up to four differ- SRD-3200 Solvent Rack with 2 Degasser Channels............................... 5035.9250
ent mobile phases HPG-3400RS Binary Rapid Separation Pump System with Solvent
Selector Valves......................................................................................... 5040.0046
• Active rear-seal wash and floating pistons for maximum
seal lifetime
The HPG-3x00RS is ideal for MS front-end configurations, Accessories

microflow, analytical, and ultrafast, high resolution separations Mixer Kit 35 µL, HPG-3x00RS Pumps...................................................... 6040.5000
in pharmaceutical, life science and environmental laboratories.
Static Mixer Kit 200 µL, HPG-3x00RS Pumps.......................................... 6040.5150
The gradient delay volume can be adjusted easily according to
your requirements (e.g., 35 µL for MS front end and 400 µL for Note: For more information on additional mixer kits, please
analytical LC) for optimal mixing efficiency. contact your local sales representative.
www.dionex.com 29
LC Modules
Isocratic Analytical Pump Ordering Information
The UltiMate 3000 Isocratic Analytical Pump ISO-3100A is

Hardware
the pump of choice for isocratic applications. It offers robust,
dependable operation to meet the demanding requirements of ISO-3100A Isocratic Analytical Pump System......................................... 5035.0010
QA and QC laboratories while making the most of your equip- SRD-3200 Solvent Rack with 2 Degasser Channels............................... 5035.9250
ment budget.
Accessories
• Serial dual-piston operating principle
UltiMate 3000 Pump Diagnostics Kit...................................................... 6035.3000
• Robust, dependable performance with low operating and Includes backpressure capillary, appropriate fittings and blind nuts for pump
maintenance costs diagnostic tests with Chromeleon Software.
• Patented piston seal leakage monitoring and System Well-

ness functions
Quaternary Analytical Pump
• Wide operating flow rate range from 50 µL/min to 10 mL/
min with high flow rate accuracy The UltiMate 3000 Quaternary Analytical Low-Pressure Gradi-
ent Pump LPG-3400A is the pump of choice for flexibility
• Support of operating pressures of up to 50 MPa (7250 psi) across a broad range of analytical LC applications, with the
highest degree of choice in eluent proportioning and mixing. Up
• Support of a wide range of column dimensions and types
to four solvents may be used for convenient method develop-
• SmartFlow technology for high flow-rate and retention- ment, system flushing, and eluent preparation.
time precision
• Serial dual-piston operating principle and low-pressure
This pump is recommended for standard isocratic applications gradient proportioning
such as those commonly used in QA/QC laboratories. It is ideal
• Reliable in-line vacuum degassing (integrated degasser)
in combination with detection techniques that do not support
and vacuum level monitoring
gradient elution, such as refractive index detection. The ISO-
3100A is also an excellent choice as an added pump for LC-MS • Wide operating flow rate range from 0.2 to 10 mL/min with
systems. high flow rate accuracy
• SmartFlow technology for high flow-rate, gradient, and
Key Specifications retention time precision
Recommended Flow Rate Range (Settable Range): 0.050– • Support of operating pressures of up to 50 MPa (7250 psi)
10.000 mL/min (0.001–10.000 mL/min)
The LPG-3400A is ideally-suited for method development, as
Flow Rate Accuracy: ±0.1% at 1 mL/min well as for analytical research and routine analysis in the phar-
Flow Rate Precision: <0.1% RSD at 1 mL/min maceutical, food and beverage, and environmental industries.
Pressure Range: 2–50 MPa (7250 psi) Note: The pump is also available in a biocompatible version
Pressure Ripple: typically <1 bar or <1%, whichever is greater (LPG-3400AB) for bioanalytical applications.
PC Connection: all functions controllable via USB; integrated Key Specifications

USB hub with three USB ports
Recommended Flow Rate Range (Settable Range): 0.200–
I/O Interfaces: three digital inputs, four programmable relays, 10.000 mL/min (0.001–10.000 mL/min)
one analog output for pressure monitoring
Flow Rate Accuracy: ±0.1% at 1 mL/min
User Input/Display: LCD indicating system parameters,
Standby button, three LEDs for status monitoring, four function Flow Rate Precision: <0.1% SD at 1 mL/min
keys for initial operation and maintenance Pressure Range: 2–50 MPa (7250 psi)
GLP Features: Automatic Instrument Qualification (AutoQ), Pressure Ripple: typically <1 bar or <1%, whichever is greater
System Wellness, and Qualification Monitoring with
Chromeleon Proportioning Accuracy: ±0.5% at 2 mL/min
Power Requirements: 100–120V, 60 Hz; 200–240V, 50 Hz Proportioning Precision: <0.2% SD at 2 mL/min
Dimensions (h × w × d): 19 × 42 × 51 cm (7.5 × 16.5 × 20 in.) Gradient Delay Volume: 690 µL (360 µL with optional micro
flow kit)
30 www.dionex.com
LC Pumps
PC Connection: all functions controllable via USB; integrated • Wide operating flow rate range from 0.2 to 10 mL/min with
USB hub with three USB ports high flow rate accuracy
I/O Interfaces: three digital inputs, four programmable relays, • SmartFlow technology for high flow rate, gradient, and
one analog output for pressure monitoring retention time precision

User Input/Display: LCD indicating system parameters, • Supports operating pressures of up to 50 MPa (7250 psi)
Standby button, three LEDs for status monitoring, four function
• Simplified setup and operation through Chromeleon
keys for initial operation and maintenance
GLP Features: Automatic Instrument Qualification (AutoQ), The DGP-3600A is recommended for research and other
System Wellness, and Qualification Monitoring with analytical laboratories that require a general purpose LC pump
Chromeleon capable of standard as well as advanced two-dimensional tech-
niques.
Power Requirements: 100-120V, 60 Hz; 200-240V, 50 Hz
Dimensions (h × w × d): 19 × 42 × 51 cm (7.5 × 16.5 × 20 in.) Note: The pump is also available in a biocompatible version
(DGP-3600AB) for bioanalytical applications.
Key Specifications
Hardware Recommended Flow Rate Range (Settable Range):

0.200–10.000 mL/min (0.001–10.000 mL/min)
LPG-3400A Quaternary Analytical Pump System.................................... 5035.0015
LPG-3400AB Biocompatible Quaternary Analytical Pump System......... 5037.0015 Flow Rate Accuracy: ±0.1% at 1 mL/min
SR-3000 Solvent Rack (without degasser).............................................. 5035.9200 Flow Rate Precision: <0.1% RSD at 1 mL/min
Pressure Range: 2–50 MPa (7250 psi)
Accessories
Micro-Flow Kit for Quaternary and Dual-Gradient Pump....................... 6035.1977 Pressure Ripple: typically <1 bar or <1% , whichever is greater
Micro-Flow Kit for Biocompatible Quaternary and Dual-Gradient Proportioning Accuracy: ±0.5% at 2 mL/min
Pump......................................................................................................... 6037.1977
Mixing Chamber Extension Kit, 600 µL.................................................... 6035.1978
Proportioning Precision: <0.2% SD at 2 mL/min
Mixing Chamber Extension Kit, 600 µL, Biocompatible.......................... 6037.1978 Gradient Delay Volume: 690 µL (360 µL with optional micro
Mixing Chamber Extension Kit, 1200 µL................................................. 6035.1979 flow kit)
Mixing Chamber Extension Kit, 1200 µL, Biocompatible........................ 6037.1979 PC Connection: all functions controllable via USB; integrated
UltiMate 3000 Pump Diagnostics Kit...................................................... 6035.3000 USB hub with three free USB ports
Includes backpressure capillary, appropriate fittings and blind nuts for pump
diagnostic tests with Chromeleon Software. I/O Interfaces: three digital inputs, four programmable relays,
Dual-Gradient Analytical Pump
The UltiMate 3000 Dual-Gradient Analytical Pump DGP- keys for initial operation and maintenance
3600A is the pump of choice for advanced analytical chromato- GLP Features: Automatic Instrument Qualification (AutoQ),
graphic techniques. It supports all standard LC applications System Wellness, and Qualification Monitoring with
and is ideal for increasing sample throughput, achieving higher Chromeleon
chromatographic resolution, or automating sample preparation
steps such as analyte enrichment or matrix elimination. Power Requirements: 100–120V, 60 Hz; 200–240V, 50 Hz
• Serial dual-piston operating principle
• Dual-gradient pump with two ternary gradient pumps in a Ordering Information
single module
• Accommodates two different applications and switches Hardware
between them without system re-plumbing
DGP-3600A Dual-Gradient Analytical Pump System.............................. 5035.0014
• Up to 100% higher sample throughput without changing DGP-3600AB Biocompatible Dual-Gradient Analytical Pump System... 5037.0014
methods (Tandem or Parallel LC mode)
www.dionex.com 31
LC Modules
Accessories Gradient Delay Volume: 375 µL
Micro-Flow Kit for Quaternary and Dual-Gradient Pump....................... 6035.1977 PC Connection: all functions controllable via USB; integrated
Micro-Flow Kit for Biocompatible Quaternary and Dual-Gradient USB hub with three USB ports
Pump......................................................................................................... 6037.1977
I/O Interfaces: three digital inputs, four programmable relays,

Mixing Chamber Extension Kit, 600 µL.................................................... 6035.1978
Mixing Chamber Extension Kit, 600 µL, Biocompatible.......................... 6037.1978
Mixing Chamber Extension Kit, 1200 µL................................................. 6035.1979 User Input/Display: LCD indicating system parameters,
Mixing Chamber Extension Kit, 1200 µL, Biocompatible........................ 6037.1979
Includes backpressure capillary, appropriate fittings and blind nuts for pump GLP Features: Automatic Instrument Qualification (AutoQ),
diagnostic tests with Chromeleon Software. System Wellness, and Qualification Monitoring with
Chromeleon
Binary Analytical Pump Power Requirements: 100–120V, 60 Hz; 200–240V, 50 Hz

The UltiMate 3000 Binary Analytical High-Pressure Gradient
Pump HPG-3x00A is ideal for analytical LC separations on
standard bore columns requiring highest gradient accuracy. In Ordering Information
addition it is also suitable for fast LC separations at pressures up
to 500 bar. SmartFlow technology enables virtually pulse-free Hardware
eluent delivery without a pulse damper.
HPG-3200A Binary Analytical Pump System........................................... 5035.0016
• Serial dual-piston operating principle SRD-3200 Solvent Rack with 2 Degasser Channels............................... 5035.9250
• Wide operating flow rate range from 0.1 to 10 mL/min with HPG-3400A Binary Analytical Pump System with Solvent Selector
Valves........................................................................................................ 5035.0017
high flow rate accuracy
• SmartFlow technology for high flow rate, gradient, and
retention time precision Accessories
• Support of operating pressures of up to 50 MPa (7250 psi) Mixing Chamber Extension Kit, 600µL..................................................... 6035.1978
• Gradient delay volume of 375 µL for fast LC applications Mixing Chamber Extension Kit, 1200µL.................................................. 6035.1979
• Mixer extensions for complete mixing even with poorly-
miscible eluents diagnostic tests with Chromeleon Software.
• Available with (HPG-3400A) and without (HPG-3200A) Note: For information on additional mixer kits, please contact
solvent selector valves your local sales representative.
The HPG-3x00A is recommended for conventional LC and

high-throughput LC applications in routine analysis in the phar- Binary Semipreparative Pump
maceutical, food and beverage, and environmental industries.
The UltiMate 3000 Binary Semipreparative Pump HPG-3200P
is the right choice for laboratories that need to purify com-
Key Specifications pounds in the microgram to lower-milligram range. It is recom-
mended for semipreparative separations on 4.6–21.2 mm i.d.
Recommended Flow Rate Range (Settable Range):
columns.
0.100–10.000 mL/min (0.001–10.000 mL/min)
Flow Rate Accuracy: ±0.1% at 1 mL/min • Serial dual-piston operating principle
Flow Rate Precision: <0.1% RSD at 1 mL/min • Wide operating flow rate range from 0.5 to 50 mL/min with
high flow rate accuracy
Pressure Ripple: typically <1 bar or <1%, whichever is greater retention time precision
Proportioning Accuracy: ±0.2% at 2 mL/min (typically • Support of operating pressures of up to 10 MPa (7250 psi)
<±0.1%)
• Gradient delay volume of 1035 µL
Proportioning Precision: <0.2% SD at 2 mL/min
32 www.dionex.com
LC Pumps
• Mixing volume extension (+1200 µL) available Quaternary Micro Pump
Typical applications include the purification of side products The UltiMate 3000 Quaternary Micro Low-Pressure Gradient
and impurities for structure elucidation, purification of com- Pump LPG-3400M is the pump of choice for a broad range

pounds from natural product extracts, and purification of com- of narrow bore LC applications with 2–3 mm i.d. columns. It
pound libraries for pharmaceutical discovery. supports use of up to four solvents for convenient and flexible
method development, system flushing, and eluent preparation.
Key Specifications • Serial dual-piston operating principle
Recommended Flow Rate Range (Settable Range): • Reliable in-line vacuum degassing (integrated degasser)
0.5–50 mL/min (0.001–50 mL/min) and vacuum level monitoring
Flow Rate Accuracy: ±0.1% at 3 mL/min (typically ±0.1% at • Wide operating flow rate range from 10 to 2500 µL/min
25 mL/min) with high flow rate accuracy
Flow Rate Precision: <0.1% RSD at 3 mL/min • SmartFlow technology for high flow rate, gradient, and
Pressure Range: 2–10 MPa (1450 psi), short term: 15 MPa retention time precision
(2100 psi) • Supports operating pressures of up to 50 MPa (7250 psi)
Pressure Ripple: typically <2 bar or <1.5%, whichever is greater • Low gradient delay volume of 230 µL
Proportioning Accuracy: ±0.2% at 5 mL/min
The LPG-3400M is ideally-suited for method development
Proportioning Precision: <0.2% SD at 5 mL/min as well as routine analysis in the pharmaceutical, food and
beverage, and environmental industries. In addition, it is a very
Gradient Delay Volume: 1035 µL
popular front end for LC-MS applications. Combine this pump
PC Connection: all functions controllable via USB; integrated with different flow managers to generate nano, capillary and
USB hub with three USB ports micro flows.

Note: The Pump is also available in a biocompatible version
(LPG-3400MB) for bioanalytical applications.
Key Specifications
Recommended Flow Rate Range (Settable Range):
10–2500 µL/min (1–2500 µL/min)
Chromeleon Flow Rate Accuracy: ±0.5% at 200 µL/min (typically ±0.25% at
200 µL/min)
Power Requirements: 136 VA (100–120V, 60 Hz; 200–240V,
50 Hz) Flow Rate Precision: <0.1% RSD at 200 µL/min
Dimensions (h × w × d): 19 × 42 × 51 cm (7.5 × 16.5 × 20 in.) Pressure Range: 2–50 MPa (7250 psi)
Pressure Ripple: typically <2 bar or <1.5%, whichever is greater
Proportioning Accuracy: ±2% at 200 µL/min (typical ±1%)
Hardware Proportioning Precision: <0.5% SD at 200 µL/min
HPG-3200P Binary Semipreparative Pump System................................ 5035.0025 Gradient Delay Volume: 230 µL
PC Connection: all functions controllable via USB; integrated
Accessories
USB hub with three free USB ports
Mixing Chamber Extension Kit, 1200µL.................................................. 6035.1979
diagnostic tests with Chromeleon Software. User Input/Display: LCD indicating system parameters,
www.dionex.com 33
LC Modules
GLP Features: Automatic Instrument Qualification (AutoQ), Note: The Pump is also available in a biocompatible version
System Wellness, and Qualification Monitoring with (DGP-3600MB) for bioanalytical applications.
Chromeleon
Power Requirements: 100–120V, 60 Hz; 200–240V, 50 Hz
Key Specifications
Dimensions (h × w × d): 19 × 42 × 51 cm (7.5 × 16.5 × 20 in.) Recommended Flow Rate Range (Settable Range):
10–2500 µL/min (1–2500 µL/min)
Ordering Information Flow Rate Accuracy: ±0.5% at 200 µL/min (typically ±0.25% at
200 µL/min)
Hardware
Flow Rate Precision: <0.1% RSD at 200 µL/min
LPG-3400M Quaternary Micro Pump System......................................... 5035.0045
LPG-3400MB Biocompatible Quaternary Micro Pump System.............. 5037.0055
SR-3000 Solvent Rack (without Degasser).............................................. 5035.9200 Pressure Ripple: typically <2 bar or <1.5%, whichever is greater
Proportioning Accuracy: ±2% at 200 µL/min (typical ±1%)
Accessories
Proportioning precision: <0.5% SD at 200 µL/min
Includes backpressure capillary, appropriate fittings and blind nuts for pump Gradient Delay Volume: 230 µL
diagnostic tests with Chromeleon Software.
PC Connection: all functions controllable via USB, integrated
Dual-Gradient Micro Pump I/O Interfaces: three digital inputs, four programmable relays,
The UltiMate 3000 Dual-Gradient Micro Pump DGP-3600M is
ideal for advanced small-bore LC techniques (1–3 mm i.d. col- User Input/Display: LCD indicating system parameters,
umns). It supports all standard LC applications and is suitable Standby button, three LEDs for status monitoring, four function
for laboratories that want to increase sample throughput, achieve keys for initial operation and maintenance
higher chromatographic resolution, or automate sample prepara-
tion steps such as analyte enrichment or matrix elimination.
• Serial dual-piston operating principle Chromeleon
• Dual-gradient pump incorporates two ternary gradient Power Requirements: 100–120V, 60 Hz; 200–240V, 50 Hz
pumps in a single LC system Dimensions (h × w × d): 19 × 42 × 51 cm (7.5 × 16.5 × 20 in.)
• Accommodates two different applications and switches
between them without system re-plumbing Ordering Information
• Up to 100% higher sample throughput without changing
methods (Tandem or Parallel LC mode) Hardware
• Wide operating flow rate range from 10 to 2500 µL/min DGP-3600M Dual-Gradient Micro Pump System.................................... 5035.0050
with high flow rate accuracy DGP-3600MB Biocompatible Dual-Gradient Micro Pump System......... 5037.0060
Accessories
• Support of operating pressures of up to 50 MPa (7250 psi)
• Simplified setup and operation through Chromeleon Includes backpressure capillary, appropriate fittings and blind nuts for pump
diagnostic tests with Chromeleon Software.
The DGP-3600M is recommended for research and other ana-
lytical laboratories that require a general purpose LC pump that
supports standard methods on 1–3 mm i.d. columns, but also
enables advanced two-dimensional techniques. Combine this
pump with different flow managers to generate nano, capillary,
and micro flows.
34 www.dionex.com
LC Pumps
Quaternary Nano/Cap/Micro Pump Flow Rate Precision: <0.1% RSD
Pressure Range: 2–50 Mpa (7250 psi)
Proportioning Accuracy: ±2% on column (typically ±1%)

Proportioning Precision: <0.5% SD on column
Effective Gradient Delay Volume: nano: 0.5 µL; cap: 3.3 µL;
micro: 50 µL
I/O interfaces: three digital inputs, four programmable relays,
The UltiMate 3000 Quaternary Nano/Cap/Micro Pump is GLP Features: Automatic Instrument Qualification (AutoQ),
equipped with the Quaternary Micro Pump LPG-3400M(B) and System Wellness, and Qualification Monitoring with
a Nano/Cap/Micro Flow Manager FLM-3x00(B). It supports Chromeleon
the accurate and precise delivery of quaternary gradients with a
Power Requirements: 100–120V, 60 Hz; 200–240V, 50 Hz
flow range of 50 nL/min–2.5 mL/min with the UltiFlow eluent
delivery system. Dimensions (h × w × d): 38 × 42 × 51 cm (15 × 16.5 × 20 in.)
• Wide flow rate range from 50 nL/min up to 2.5 mL/min

(depending on split ratio) Ordering Information
• Easily convertable between nano, capillary and micro flow The UltiMate 3000 Quaternary Nano/Cap/Micro Pump consists
rates of the LPG-3400M(B) Quaternary Micro Pump and FLM-
3x00(B) Nano/Cap/Micro Flow Manager with the UltiFlow
• UltiFlow technology for constant flow independent of elu- eluent delivery system delivering the appropriate flow rate.
ent composition and backpressure The pump can be easily converted between nano, capillary, and
• Supports use of up to four solvents for gradient applications micro flow rates by exchanging the flow splitter of the FLM-
3x00(B). For order information on the Flow Manager and Flow
• Active rear-seal wash system for increased pump uptime Splitter, please refer to the Flow Managers section.
• Superior gradient accuracy and precision
• Biocompatible option
The UltiMate 3000 Quaternary Nano Pump is used primarily as

front-end separation system for mass spectrometry in proteomic
and bioanalytical analyses. The Cap/Micro pump variant is
recommended for research and routine analyses of small sample
amounts in drug discovery applications, particularly for drug
metabolism and pharmacokinetic studies.
Key Specifications
Recommended Flow Rate Range: nano: 50–1000 nL/min;
cap: 0.5–10 µL/min, micro: 10–160 µL/min
Flow Rate Accuracy: nano: ±3% at 300 nL/min, cap: ±1.5% at
4 µL/min; micro: ±1% at 50 µL/min
www.dionex.com 35
LC Modules
Dual-Gradient Nano/Cap/Micro Pump Proportioning Accuracy: ±2% on column (typically ±1%)
Proportioning Precision: <0.5% SD on column
Effective Gradient Delay Volume: nano: 0.5 µL; cap: 3.3 µL;
micro: 50 µL
Chromeleon
The UltiMate 3000 Dual-Gradient Nano/Cap/Micro Pump Power Requirements: 100-120V, 60 Hz; 200-240V, 50 Hz
is equipped with the DGP-3600M(B) Dual-Gradient Micro
Pump and a FLM-3x00(B) Nano/Cap/Micro Flow Manager. Dimensions (h × w × d): 38 × 42 × 51 cm (15 × 16.5 × 20 in.)
It provides two independent ternary low-pressure gradients;
the UltiFlow eluent delivery system allows flow rates down to Ordering Information
50 nL/min.
The Dual-Gradient Nano/Cap/Micro Pump consists of a DGP-
• Wide flow rate range from 50 nL/min to 2.5 mL/min (de- 3600M(B) Dual-Gradient Micro Pump and FLM-3x00 Nano/
pending on split ratio) Cap/Micro Flow Manager with the UltiFlow eluent delivery
• Dual-gradient pump with two independent ternary gradient system. The pump can be easily converted between nano, capil-
pumps lary, and micro flow rates by exchanging the flow splitter of the
FLM-3x00(B). For order information on the Flow Manager and
• Easily convertable between nano, capillary and micro flow Flow Splitter, please refer to the Flow Managers section.
rates
• UltiFlow technology for constant flow independent of elu-
ent composition and backpressure
• Active rear-seal wash system for increased pump uptime
• Superior gradient accuracy and precision
• Biocompatible option
The Dual-Gradient Nano/Cap/Micro Pump is used primarily for

bioanalytical studies such as MS-based proteomics. It enables
preconcentration, on-line and off-line multidimensional LC
applications and supports complex proteomic workflows such as
protein pre-fractionation, protein/peptide separation, and peptide
mapping. It is an essential part of the Proteomic MDLC system.
Key Specifications
Recommended Flow Rate Range: nano: 50–1000 nL/min;
cap: 0.5–10 µL/min; micro: 10–160 µL/min
Flow Rate Accuracy: nano: ±3% at 300 nL/min;
cap: ±1.5% at 4 µL/min; micro: ±1% at 50 µL/min
Flow Rate Precision: <0.1% RSD
Pressure Range: 2–50 Mpa (7250 psi)
36 www.dionex.com
LC Injectors & Autosamplers
LC Injectors & Autosamplers Related Literature

Product Data Sheets
UltiMate 3000 Autosampler Series
RSLC Autosampler
The UltiMate 3000 Autosampler Series features innovative and

carefully-designed autosampler modules that ensure reliable,
precise, and accurate injection for nL to mL sample volumes.
Select the right autosampler module to match your LC applica-
tion whether it requires a nano, capillary, micro, analytical, or
semi-preparative column and system configuration.
• Fast injection cycles of <15 s at 5 µL injection volume

• Supports simultaneous use of up to four different sample
formats, including well plates
• Patented sample compartment temperature control to pro- The UltiMate 3000 Rapid Separation Autosampler injects
tect thermally sensitive samples sample volumes up to 100 µL at pressures up to 800 bar
• Easy access to all fluidic components through the flip-up (11,600 psi). The in-line, split-loop injection principle provides
front door panel highly accurate and precise delivery of the sample with ultralow
carryover. The wide injection volume range makes the module
• Precision mechanical syringe drives for injection volume suitable for both UHPLC and conventional HPLC applications.
accuracies of ±0.5%
• Peak area precision is typically <0.15% RSD for 5 µL
• Automated validation procedures with Chromeleon soft- injections
ware
• Superior injector linearity of r2 >0.9999 (5-90 µL) due to
• Predefined diagnostic tests to help identify and resolve high-precision drive mechanism
operational interruptions
• Wide injection volume range from 1–100 µL (default con-
• Autosamplers available with either pulled-loop or in-line figuration) for maximum injection flexibility
split-loop injection principle
• Optional injection volume ranges of 0.2–25 µL,
• Very low extracolumn volume contribution 1.5–200 µL, and 1.5–500 µL available
Each UltiMate 3000 autosampler provides a highly-integrated • Low gradient delay volume of 140 µL (default configura-
solution with optimum fluidic connections, single-point control tion) or 50 µL (with 25 µL sample loop)
through Chromeleon Chromatography Management Software,
and seamless inter-module communication. The ACC-3000 • Volumetric accuracy better than ±0.5% for 20 µL injections
Autosampler Column Compartment features an integrated for easy method transfer
column oven and provides a cost-effective solution for routine • Supports well plates and short injection cycle times for
analyses. high-throughput applications
• Sample thermostatting for optimal protection of thermally-
sensitive analytes (WPS-3000TRS)
www.dionex.com 37
LC Modules
The UltiMate 3000 Rapid Separation Autosampler offers mini- Accessories
mal gradient delay and low extracolumn volume which provide
Sample Loop, 25 µL, WPS-3000RS and WPS-3000SL Analytical
high efficiency separations and short chromatographic run Samplers................................................................................................... 6820.2415
times. It is strongly recommended for ultrahigh pressure, high-
Injection Volume Kit, 250 µL, WPS-3000RS and WPS-3000SL
throughput and high-resolution applications and is available in Analytical Samplers................................................................................. 6822.2432

a non-thermostatted (WPS-3000RS) and thermostatted version
Injection Volume Kit, 500 µL, WPS-3000RS and WPS-3000SL
(WPS-3000TRS).
Analytical Samplers................................................................................. 6822.2433
Sample Tray for 72 Cylindrical 0.3 mL Vials............................................ 6820.4091
Key Specifications Sample Tray for 40 Conical 1.1 mL Vials................................................. 6820.4087
Sample Capacity, Vials: 216 × 0.3 mL, 120 × 1.1 mL Sample Tray for 72 Cylindrical 1.2 mL Vials............................................ 6820.4090
(conical), 216 × 1.2 mL, 120 × 1.8 mL/2.0 mL, 66 × 4 mL, and/ Sample Tray for 40 Cylindrical 1.8 mL/2.0 mL Vials................................ 6820.4070
or 30 × 10 mL + 15 × 10 mL Sample Tray for 22 Cylindrical 4 mL Vials............................................... 6820.4084
Sample Tray for 10 Cylindrical 10 mL Vials............................................. 6820.4086
Sample Capacity, Well Plates: 3 × 96 and/or 384 normal and
deep well plates Sample Support Rack for Deep Well Plates............................................ 6820.4079
Transparent Front Cover........................................................................... 6820.1419
Injection Method: in-line split-loop injections, bypass mode,
user-defined programs
Analytical Autosampler
Injection Volume Range (Recommended): 0.01–100 µL
(1–100 µL) The UltiMate 3000 Analytical Autosampler WPS-3000(T)SL is
Minimum Sample Required: 1 µL out of 5 µL (250 µL conical highly versatile in sample format and injection volume. It per-
vial) forms rapid, accurate, and precise injections for analytical LC
applications. The in-line, split-loop injection principle (needle
Injection Volume Accuracy: ±0.5% at 20 µL and sample loop integral to the high-pressure fluidic path) and
Injection Volume Precison: <0.25% RSD at 5 µL (typically external needle wash options virtually eliminate carry-over.
<0.15% RSD), caffeine in water
• Peak area precision typically <0.15% RSD for 5 µL
Linearity: corr. coeff. >0.9999, RSD <0.5% at 5–90 µL, caffeine injections
in water
• Superior injector linearity of r²=0.999 (5–90 µL) due to a
Needle Wash: active external high-precision drive mechanism
Carryover: <0.005% for caffeine with external wash at 20 MPa • Low gradient delay volume of 140 µL (default configura-
tion) or 50 µL (with 25 µL sample loop)
Injection Cycle Time: <15 s for 5 µL
• Superior volumetric accuracy of better than ±0.5% for
Sample Thermostatting: 4–45 ºC or 22 ºC below ambient consistent results
Sample Thermostatting Accuracy: ±2 ºC • Supports an injection volume range from 1–100 µL,
PC Connection: all functions controllable via USB; integrated suitable for most analytical applications
USB hub with three USB 1.1 ports • Optional injection volume ranges of 0.2–25 µL,
Dimensions (h × w × d): 36 × 42 × 51 cm (14.2 × 16.5 × 20 in.) 1.5–200 µL, and 1.5–500 µL available
• Supports well plates and short injection cycle times for
high-throughput applications
• Sample thermostatting for optimal protection of thermally
sensitive analytes (WPS-3000TSL)
Hardware
WPS-3000RS Rapid Separation In-Line Split-Loop Autosampler.......... 5840.0010 The UltiMate 3000 Analytical Autosampler offers robust,
WPS-3000TRS Rapid Separation Thermostatted Autosampler............. 5840.0020 dependable performance as well as high flexibility with low
operating and maintenance costs. It is ideally suited for routine
analyses in pharmaceutical, food and beverage, and environ-
mental laboratories, and is available in a non-thermostatted
(WPS-3000SL) and thermostatted versions (WPS-3000TSL).
38 www.dionex.com
Key Specifications Accessories
Sample Capacity, Vials: 216 × 0.3 mL, 120 × 1.1 mL Sample Loop, 25 µL, WPS-3000RS and WPS-3000SL Analytical
(conical), 216 × 1.2 mL, 120 × 1.8 mL/2.0 mL, 66 × 4 mL, and/ Samplers................................................................................................... 6820.2415
or 30 × 10 mL + 15 × 10 mL Injection Volume Kit, 250 µL, WPS-3000RS and WPS-3000SL

Analytical Samplers................................................................................. 6822.2432
Sample Capacity, Well Plates: 3 × 96 and/or 384 normal and Injection Volume Kit, 500 µL, WPS-3000RS and WPS-3000SL
deep well plates Analytical Samplers................................................................................. 6822.2433
Injection Method: in-line split-loop injections, bypass mode, Sample Tray for 72 Cylindrical 0.3 mL Vials............................................ 6820.4091
user-defined programs Sample Tray for 40 Conical 1.1 mL Vials................................................. 6820.4087
(1–100 µL) Sample Tray for 40 Cylindrical 1.8 mL/2.0 mL Vials................................ 6820.4070
Sample Tray for 22 Cylindrical 4 mL Vials............................................... 6820.4084
Minimum Sample Required: 1 µL out of 5 µL (250 µL conical
vial)
Sample Support Rack for Deep Well Plates............................................ 6820.4079
Injection Volume Accuracy: ±0.5% at 50 and 90 µL Transparent Front Cover........................................................................... 6820.1419
Injection Volume Precision: <0.25% RSD at 5 µL (typically
<0.15% RSD), caffeine in water
Biocompatible Analytical Autosampler
Linearity: corr. coeff. >0.9999, RSD <0.5% at 5–90 µL, caffeine
in water The UltiMate 3000 Analytical Autosampler WPS-3000TBPL
has been specifically designed to provide rapid, accurate, and
Needle Wash: active external precise injections of complex samples containing biomolecules.
Carryover: <0.005% for caffeine with external wash at 7.5 MPa It features low gradient delay volume and ultralow carryover.
Injection Cycle Time: <15 s for 5 µL • Pulled-loop injection principle, full- and partial-loop injec-
tions possible
Sample Thermostatting: 4–45 ºC or 22 ºC below ambient
• Dual-needle injection technique for robust operation with
Sample Temperature Accuracy: ±2 ºC different sample formats
PC Connection: all functions controllable via USB; integrated • Inert PEEK flow paths
USB hub with three USB 1.1 ports
• Supports up to three 96- or 384-well plates or sample vial
Dimensions (h × w × d): 36 × 42 × 51 cm (14.2 × 16.5 × 20 in.) trays, or a combination thereof
• Wide injection volume ranges from 0.1–50 µL or
Ordering Information 0.25–250 µL for maximum flexibility
• Sample tray thermostatting and cover for injection of
Hardware thermo- and UV-labile samples
WPS-3000SL Analytical In-Line Split-Loop Autosampler....................... 5822.0010 • Low gradient delay volume
WPS-3000TSL Analytical In-Line Split-Loop Thermostatted
Autosampler............................................................................................. 5822.0020 The UltiMate 3000 Analytical Autosampler is ideally-suited for
analytical LC applications requiring biocompatibility. As part of
the UltiMate 3000 x2 Dual Titanium System, this autosampler
helps to support multidimensional analysis and purification of
proteins.
Key Specifications
Sample Capacity, Vials: 216 × 0.3 mL, 120 × 1.1 mL
(conical), 216 × 1.2 mL, 120 × 1.8 mL/2.0 mL, 66 × 4 mL, and/
or 30 × 10 mL + 15 × 10 mL
Sample Capacity, Well Plates: 3 × 96 and/or 384 normal or deep
well plates
www.dionex.com 39
LC Modules
Injection Methods: pulled-loop injections (full-loop and partial- Autosampler Column Compartment
loop mode), low-dispersion mode, microliter pickup, user-
defined programs
(0.1–250 µL)
Minimum Sample Required: 1 µL out of 1 µL (microliter
pickup)
Injection Volume Precision: <0.25% RSD at 5 µL in full-loop
and <0.3% RSD at 5 and 20 µL in partial-loop mode
Linearity: corr. coeff. >0.9999, RSD <0.5% at 5–30 µL (partial-
loop mode), caffeine in water
Needle Wash: active external
Carryover: <0.02% for caffeine with external wash
Injection Cycle Time: <60 s for 5 µL full-loop injection, <90 s
for 5 µL partial-loop injection The UltiMate 3000 Autosampler Column Compartment
ACC‑3000(T) provides a cost-effective solution ideally suited
Sample Thermostatting: 4–45 ºC, or 22 ºC below ambient for routine analyses in many industrial fields. It integrates an
autosampler and column oven in a single module and offers
Sample Temperature Accuracy: ± 2 ºC
performance and flexibility that exceed most analytical method
PC Connection: all functions controllable via USB; integrated requirements. In addition, it reduces height and cost of your
USB hub with three USB 1.1 ports LC system.
Dimensions (h × w × d): 36 × 42 × 51 cm (14.2 × 16.5 × 20 in.) • Pulled-loop injection principle (full- and partial-loop injec-
tions possible)
Ordering Information • Wide injection volume ranges from 1–10 µL in partial-loop
and 20 µL in full-loop mode or 10–100 µL and 200 µL,
Hardware respectively
WPS-3000TBPL Biocompatible Analytical Pulled-Loop Thermostatted • Low sample carryover
Autosampler............................................................................................. 5823.0020
• Patented temperature control to protect thermally sensitive
Accessories samples
Needle, 15 µL, biocompatible, WPS-3000PL Nano/Cap and • Accurate and precise column temperature control for
WPS‑3000TBPL Analytical....................................................................... 6821.3115 reliable chromatographic results
Sample Loop, 5 µL, biocompatible, PEEK, WPS-3000TBPL Analytical... 6823.0016 • Built-in eluent preheating for increased chromatographic
Sample Loop, 10 µL, biocompatible, PEEK, WPS-3000TBPL Analytical.6823.0017 reliability at high temperatures
Sample Loop, 20 µL, biocompatible, PEEK, WPS-3000TBPL Analytical.6823.0018
• Easy front access to all fluidic components
Sample Tray for 40 Conical 1.1 mL Vials................................................. 6820.4087 • Trouble-free operation due to minimized number of wear
parts
Sample Tray for 40 Cylindrical 1.8 mL/2.0 mL Vials................................ 6820.4070 The UltiMate 3000 Autosampler Column Compartment is rec-
Sample Tray for 22 Cylindrical 4 mL Vials............................................... 6820.4084 ommended for research and other analytical laboratories dealing
Sample Tray for 10 Cylindrical 10 mL Vials............................................. 6820.4086 in a cost-sensitive environment. It is available in non-thermo-
Sample Support Rack for Deep Well Plates............................................ 6820.4079 statted (ACC-3000) and thermostatted (ACC-3000T) versions
and is an essential part of the UltiMate 3000 Basic Automated
System.
40 www.dionex.com
Key Specifications Semipreparative Autosampler
Sample Capacity, Vials: 216 × 0.3 mL, 120 × 1.1 mL
The UltiMate 3000 Semipreparative Autosampler WPS-3000(T)
SL performs reliable, accurate, and precise injections in semi-
or 30 × 10 mL + 15 × 10 mL

preparative LC applications. It is designed to deliver mL injec-
Injection Method: pulled loop injections (full-loop and partial- tion volumes while minimizing backpressure contributions. The
loop mode) in-line, split-loop injection principle and external needle wash
options virtually eliminate carry-over.
Full-Loop Injection Volume: 20, 50, and 200 µL
• Robust, dependable performance with low operating and
Injection Volume Range (Recommended): 0–50% (10–50%) of maintenance costs
installed sample loop in partial-loop mode
• Injection volume range from 100–2500 µL (default) or
Injection Volume Precision: <0.25% RSD at 20 µL in full-loop 10–1000 µL (optional)
mode, typically <0.3% at 5 µL in partial-loop mode (20 µL
sample loop) • Superior flow path design for flow rates up to 50 mL/min at
low backpressures
Linearity (20 µL Sample Loop): corr. coeff. >0.9995, RSD <1%
for 1–10 µL injections in partial-loop mode • 66 × 4 mL or 45 × 10 mL vial formats for multiple large
volume injections of the same sample
Linearity (200 µL Sample Loop): corr. coeff. >0.9995,
RSD <0.5% for 10–100 µL injections in partial loop mode • Sample thermostatting for optimal protection of thermally
sensitive analytes (WPS-3000TSL)
Carryover: <0.02% with caffeine The Ultimate 3000 Semipreparative Autosampler is recom-
mended for all semi-preparative LC instrument configurations
Injection Cycle Time: typically <45 seconds with 5 µL injections and is available in a non-thermostatted (WPS-3000SL) and
thermostatted version (WPS-3000TSL).
Column Temperature Range: 5 ºC above ambient to 50 ºC Key Specifications
PC Connection: all functions controllable via USB; integrated Sample Capacity, Vials: 216 × 0.3 mL, 120 × 1.1 mL
USB hub with three USB 1.1 ports (conical), 216 × 1.2 mL, 120 × 1.8 mL/2.0 mL, 66 × 4 mL, and/
Dimensions (h × w × d): 36 × 42 × 51 cm (14.2 × 16.5 × 20 in.) or 30 × 10 mL + 15 × 10 mL
Sample Capacity, Well Plates: 3 × 96 and/or 384 normal or deep
well plates
Injection Method: in-line split-loop injections, bypass mode,
user-defined programs
Hardware
ACC-3000 Autosampler Column Compartment....................................... 5830.0010
(100–2500 µL)
ACC-3000T Autosampler Column Compartment with Sample
Thermostatting......................................................................................... 5830.0020 Injection Volume Accuracy: ±1% at 2000 µL
Injection Volume Precision: <0.3% RSD at 100 µL (typically
Accessories <0.15% RSD), caffeine in water
Sample Loop, 50 µL, ACC-3000 Autosampler Column Compartment.... 6830.2442 Linearity: corr. coeff. >0.9995, RSD <1% at 100–2000 µL, caf-
Syringe, 1000 µL, WPS-3000 and ACC-3000 Series............................... 6822.0005 feine in water
Sample Tray for 40 Conical 1.1 mL Vials................................................. 6820.4087
Sample Tray for 72 Cylindrical 1.2 mL Vials............................................ 6820.4090 Carryover: <0.005% for caffeine with external wash at 7.5 MPa
Sample Tray for 40 Cylindrical 1.8 mL/2.0 mL Vials................................ 6820.4070 Injection Cycle Time: <20 s for 100 µL
Sample Thermostatting Accuracy: ±2 ºC
USB hub with three USB 1.1 ports
www.dionex.com 41
LC Modules
Ordering Information Note: For more information on the Micro Fraction Collection
Option, please refer to the Fraction Collection section under
LC Modules.
Hardware
WPS-3000SL Semipreparative In-Line Split-Loop Autosampler............ 5822.0018 Key Specifications

WPS-3000TSL Semipreparative In-Line Split-Loop Thermostatted
Autosampler............................................................................................. 5822.0028 Sample Capacity, Vials: 216 × 0.3 mL, 120 × 1.1 mL
or 30 × 10 mL + 15 × 10 mL
Accessories
Injection Volume Kit, 1000 µL, WPS-3000SL Semipreparative.............. 6820.2436 Sample Capacity, Well Plates: 3 × 96 and/or 384 normal or deep
well plates
Sample Tray for 40 Conical 1.1 mL Vials................................................. 6820.4087 Injection Method: pulled-loop injections (full-loop and partial-
Sample Tray for 72 Cylindrical 1.2 mL Vials............................................ 6820.4090 loop mode), low-dispersion mode, microliter pickup, user-
Sample Tray for 40 Cylindrical 1.8 mL/2.0 mL Vials................................ 6820.4070
defined programs
Sample Tray for 22 Cylindrical 4 mL Vials............................................... 6820.4084 Injection Volume Range (Recommended): 0.01–5 µL
Sample Tray for 10 Cylindrical 10 mL Vials............................................. 6820.4086 (0.02–5 µL)
Sample Support Rack for Deep Well Plates............................................ 6820.4079 Minimum Sample Required: 1 µL out of 1 µL (microliter
Transparent Front Cover........................................................................... 6820.1419 pickup)
Injection Volume Precison: <0.4% RSD for 1 µL in full-loop
Nano/Cap Autosampler and <1% for 200 nL in partial-loop injection mode, caffeine in
water
The UltiMate 3000 Nano/Cap Autosamplers WPS-3000(T)PL
and WPS-3000TBPL (biocompatible) have been specifically Linearity: corr. coeff. >0.9995, at 100–500 nL (partial-loop
designed to provide reliable unattended sample throughput and mode), caffeine in water
handling of nL sample volumes. They are ideal for LC appli- Needle Wash: active external
cations at nano, capillary and micro flow rates accompanying
highest injection precision and zero sample loss. Carryover: <0.02% for caffeine with external wash
Injection Cycle Time: <30 s for 1 µL full-loop injections
• Pulled-loop injection principle, full-loop and low dispersion
partial-loop injections possible Sample Thermostatting: 4–45 ºC or 22 ºC below ambient
• Dual-needle injection technique for robust operation with Sample Thermostatting Accuracy: ±2 ºC
different sample formats
• Handles up to three 96- or 384-well plates or sample vial USB hub with three USB 1.1 ports
trays, or combination thereof
• Injection volume range from 0.02–25 µL (default),
0.02–20 µL, or 0.1–125 µL
• Sample tray thermostatting and cover for injection of
thermo- and UV-labile samples For an injection volume range up to 20 µL, the 20 µL sample
loop in combination with a 100 µL syringe and 500 µL buffer
• External needle wash for lowest carryover of typically tubing is recommended.
<0.01%
• µL-Pickup routine for injections with virtually zero sample Hardware
loss
WPS-3000PL Nano/Cap Pulled-Loop Autosampler................................ 5820.0010
• Micro Fraction Collection option for automated off-line WPS-3000TPL Nano/Cap Pulled-Loop Thermostatted Autosampler..... 5820.0020
multidimensional LC (MDLC)
WPS-3000TBPL Biocompatible Nano/Cap Pulled-Loop Thermostatted
Autosampler............................................................................................. 5821.0020
The UltiMate 3000 Nano/Cap Autosampler is ideally suited for
LC/MS applications. Biocompatibility and sample thermostat-
ting qualify the instrument for all small-scale bioanalytical ap-
plications. It is available in a non-thermostatted (WPS-3000PL)
and thermostatted (WPS-3000TPL) as well as a thermostatted
biocompatible (WPS-3000TBPL) version.
42 www.dionex.com
Accessories • Conveniently mounted to your UltiMate 3000 system
Needle, 15 µL, Fused Silica, WPS-3000PL Nano/Cap Samplers............ 6820.3115
The Analytical Manual Injector Kit includes a 20 µL sample
Needle, 15 µL, Biocompatible, WPS-3000PL Nano/Cap and WPS- loop, while the Semi-preparative Manual Injector includes
3000TBPL Analytical................................................................................. 6821.3115

20 µL and 2 mL sample loops. Both kits contain a holder for
Sample Loop, 10 µL, PEEKsil, WPS-3000PL Nano/Cap Samplers.......... 6820.0017 the magnetic pen needed for direct control of UltiMate 3000
Sample Loop, 10 µL, Biocompatible, PEEKsil, WPS-3000PL Nano/Cap modules, for example during maintenance.
Samplers................................................................................................... 6821.0017
Sample Loop, 20 µL, PEEK, WPS-3000PL Nano/Cap Samplers.............. 6820.0018
Key Specifications
Sample Loop, 20 µL, Biocompatible, PEEKsil, WPS-3000PL Nano/Cap
Samplers................................................................................................... 6821.0018 Full-Loop Injection Volume: 20 µL or 2 mL
Buffer Tubing, 500 µL, WPS-3000PL Nano/Cap Samplers..................... 6820.0020
Maximum Pressure: 48 Mpa (7000 psi)
Buffer Tubing, 500 µL, Biocompatible, WPS-3000PL Nano/Cap
Samplers................................................................................................... 6821.0020 Wetted Surfaces (Analytical Version): 316 stainless steel, inert
Syringe, 100 µL, WPS-3000PL Nano/Cap Samplers............................... 6822.0002 polymer
Injection Volume Kit, 125 µL, WPS-3000PL Nano/Cap Samplers.......... 6820.0031
Wetted Surfaces (Semipreparative Version): 316 SST, alumina
Injection Volume Kit, Biocompatible, 125 µL, WPS-3000PL Nano/Cap ceramic, inert polymer
Samplers................................................................................................... 6821.0031
Sample Tray for 72 Cylindrical 0.3 mL Vials............................................ 6820.4091 pH Range: 0–10
Sample Tray for 40 Conical 1.1 mL Vials................................................. 6820.4087 Maximum Temperature: 80 ºC
Flow Passage Diameters (Analytical Version): 0.33 mm
Sample Tray for 40 Cylindrical 1.8 mL/2.0 mL Vials................................ 6820.4070
(0.013 in.) and 0.30 mm (0.012 in.)
Flow Passage Diameters (Semipreparative Version): 0.6 mm
(0.024 in.) and 0.5 mm (0.018 in.)
Sample Support Rack for Deep Well Plates............................................ 6820.4079
Manual Injectors Hardware

Manual Injection Valve Kit, Analytical, UltiMate 3000 Pumps.............. 5035.0600
Manual Injection Valve Kit, Preparative, UltiMate 3000 Pumps............ 5035.0601
Accessories
Syringe, 100 µL, Manual Injectors........................................................... 6035.0665
Syringe, 5 mL, Manual Injectors.............................................................. 6035.0670
Mounting Kit for Manual Injection Valves, UltiMate 3000 Pumps........ 6035.0610
Manual Injector Kits are available in two versions, for analyti-

cal- and semipreparative-scale injections. The kits include valve,
mounting brackets for connection to UltiMate pumps, and all
tubes, ferrules, nuts, and other parts needed for fluidic connec-
tions between pump, valve, and column.
• Start signal generated for data acquisition

• Proven ceramic parts to ensure long injection valve life
• Supports full-loop and partial-loop injections
www.dionex.com 43
LC Modules
LC Flow Managers Related Literature
Product Data Sheets
UltiMate 3000 Pumps Datasheet
Flow Manager with Two 2P-10P Valves

The UltiMate 3000 Flow Manager FLM-3100 includes two
2-position 10-port switching valves, which enable 2-D LC Salt
Plug and Off-line 2-D LC. The flow manager features the Ulti-
Flow eluent delivery system for nano, capillary, and micro flow
rates, and is also available in a biocompatible version (FLM-
3100B).
The UltiMate 3000 Flow Manager FLM-3000 series features
the UltiFlow eluent delivery system for nano, capillary, and mi- • Column thermostatting from 5 to 70 ºC
cro flow rates. It integrates column thermostatting and includes • Short warm-up/cool-down times for fast temperature stabi-
up to two low-dispersion switching valves for automated sample lization
preparation and multidimensional LC applications.
• Column capacity of up to three microcolumns (max length:
• UltiFlow technology for nano, capillary, and micro flow 30 cm)
rates (50 nL/min–160 µL/min)
• UltiFlow technology for nano, capillary and micro flow
• Electronical nano, capillary and micro flow control for high rates (50 nL/min-160 µL/min)
The UltiMate 3000 Flow Manager FLM-3100(B) can be eas-
• Easy change of flow rate range (nano, cap, or micro) by ily converted between nano, capillary and micro flow rates by
exchanging the splitter cartridge exchanging the flow splitter cartridge.
• Column thermostatting from 5 to 70 ºC, (max. 15 ºC below
ambient) Note: For more details on Proteomics MDLC 2-D Salt Plug
and Proteomics MDLC Off-line 2-D LC, please refer to the LCi
• Short warm-up/cool-down times for fast temperature stabi- Solutions section.
lization
Key Specifications
30 cm)
Temperature Range: 5–70 ºC or 15 ºC below ambient
• Thermostatted microswitching valves for maximum ap-
plication flexibility Temperature Accuracy: ±0.5 ºC
• Biocompatible versions for delicate biomolecule analyses Temperature Stability and Precision: ±0.1 ºC
and high salt applications
Heat-Up and Cool-Down Time: from 20 ºC to 50 ºC in less than
• Enables Proteomics MDLC solutions 25 min, from 50 ºC to 20 ºC in less than 35 min
The FLM, in combination with a Quaternary or Dual-Gradient Switching Valves: two low-dispersion thermostatted 2-position
Micro Pump, provides accurate and precise gradient formation, 10-port micro valves
short gradient delay times, and smooth baselines. The pump Column Capacity: up to three columns, max. 30 cm length
integrates proportioning and mixing of up to three eluents at
flow rates of 50 to 1000 µL while the UltiFlow eluent delivery Flow Splitter/Control: thermostatted nano, capillary, or micro
system splits the flow to nano, capillary, or micro flow rates. splitter with automatic identification system
Flow Range Selection: 50–1000 nL/min (nano), 0.5–10 µL/min
Note: For more details on the Quaternary or Dual-Gradient (capillary), 10–160 µL/min (micro flow splitter)
Micro Pumps, please see the LC pump section.
Gradient Delay Time: typically <1.5 min at 200 nL/min to
splitter outlet
44 www.dionex.com
LC Flow Managers
Maximum Column Pressure: 35 MPa (4900 psi) • UltiFlow technology for nano, capillary and micro flow
rates (50 nL/min–160 µL/min)
I/O Interfaces: two digital inputs, two programmable relay
outputs The UltiMate 3000 Flow Manager FLM-3200 can easily be con-

User Input/Display: LCD indicating system parameters, standby verted between nano, capillary and micro flow rates by simply
button, three LEDs for status monitoring, four function keys for exchanging the flow splitter cartridge.
initial operation and maintenance
Note: For more details on Proteomics Parallel LC, please
GLP Features: Column Identification System, Automatic Equip- refer to the LCi solution section.
ment Qualification (AutoQ), System Wellness, and Qualification
Monitoring with Chromeleon
Key Specifications
Power Requirements: 162 VA, automatic voltage selection
Dimensions (h × w × d): 17 × 42 × 51 cm (6.7 × 16.5 × 20 in)
Temperature Accuracy: ±0.5 ºC
Ordering Information Temperature Stability and Precision: ±0.1 ºC
Heat-Up and Cool-Down Time: from 20 ºC to 50 ºC in less than
Hardware 25 min, from 50 ºC to 20 ºC in less than 35 min
FLM-3100, Nano, 2X2P-10P Flow Manager with Two 2-Position
Switching Valves: low-dispersion thermostatted 2-position
10‑Port Switching Valves......................................................................... 5720.0010
10‑port micro and 2-position 6-port nano valve
FLM-3100, Cap, 2X2P-10P Flow Manager with Two 2-Position 10-Port
Switching Valves...................................................................................... 5720.0015 Column Capacity: up to three columns, max. 30 cm length
FLM-3100, Micro, 2X2P-10P Flow Manager with Two 2-Position
Flow Splitter/Control: thermostatted nano, capillary, or micro
10‑Port Switching Valves......................................................................... 5720.0018
splitter with automatic identification system
FLM-3100B, Nano, 2X2P-10P Biocompatible Flow Manager with Two
2-Position 10-Port Switching Valves........................................................ 5721.0010 Flow Range Relection: 50–1000 nL/min (nano), 0.5–10 µL/min
(capillary), 10–160 µL/min (micro flow splitter)
Accessories
Gradient Delay Time: typically <1.5 min at 200 nL/min to
Upgrade Kit to CAP LC, without Flow Cell, FLM Flow Managers.......... 6720.0095 splitter outlet
Upgrade Kit to Micro LC, without Flow Cell, FLM Flow Managers........ 6720.0097
Maximum Column Pressure: 35 MPa (4900 psi)
Upgrade kit to Nano LC, without Flow Cell, FLM Flow Managers......... 6720.0099
Upgrade Kit to CAP LC, Biocompatible, without Flow Cell, FLM Flow I/O Interfaces: two digital inputs, two programmable relay
Managers.................................................................................................. 6721.0095 outputs
Upgrade Kit to Micro LC, Biocompatible, without Flow Cell, FLM Flow User Input/Display: LCD indicating system parameters, standby
Managers.................................................................................................. 6721.0097
button, three LEDs for status monitoring, four function keys for
Upgrade Kit to Nano LC, Biocompatible, without Flow Cell, FLM Flow initial operation and maintenance
Managers.................................................................................................. 6721.0099
Column Identification System with Chip Cards, 5 pcs............................ 6710.1505 GLP Features: Column Identification System, Automatic Equip-
Flow Manager with 2P-10P/2P-6P Valve
Power Requirements: 162 VA, automatic voltage selection
The UltiMate 3000 Flow Manager FLM-3200 includes a 2-posi- Dimensions (h × w × d): 17 × 42 × 51 cm (6.7 × 16.5 × 20 in)
tion 10-port micro and a 2-position 6-port nano switching valve,
which enable Parallel LC (in combination with FLM-3300). The
flow manager features the UltiFlow eluent delivery system for Ordering Information
nano, capillary, and micro flow rates, and is also available in a
biocompatible version (FLM-3200B). Hardware
• Column thermostatting from 5 to 70 ºC FLM-3200, Nano, 2X2P-10P Flow Manager with 2-Position 10-Port
and 2-Position 6-Port Switching Valves.................................................. 5720.0020
• Short warm-up/cool-down times for fast temperature stabi- FLM-3200B, Nano, 2X2P-10P Biocompatible Flow Manager with
lization 2-Position 10-Port and 2-Position 6-Port Switching Valves.................... 5721.0020

30 cm)
www.dionex.com 45
LC Modules
Accessories Flow Splitter/Control: thermostatted nano, capillary, or micro
splitter with automatic identification system
Upgrade Kit to CAP LC, without Flow Cell, FLM Flow Managers.......... 6720.0095
Upgrade Kit to Micro LC, without Flow Cell, FLM Flow Managers........ 6720.0097 Flow Range Selection: 50–1000 nL/min (nano), 0.5–10 µL/min
(capillary), 10–160 µL/min (micro flow splitter)
Upgrade kit to Nano LC, without Flow Cell, FLM Flow Managers......... 6720.0099
Upgrade Kit to CAP LC, Biocompatible, without Flow Cell, FLM Flow Gradient Delay Time: typically <1.5 min at 200 nL/min to
Managers.................................................................................................. 6721.0095 splitter outlet
Upgrade Kit to Micro LC, Biocompatible, without Flow Cell, FLM Flow
Managers.................................................................................................. 6721.0097 Maximum Column Pressure: 35 MPa (4900 psi)
Upgrade Kit to Nano LC, Biocompatible, without Flow Cell, FLM Flow I/O Interfaces: two digital inputs, two programmable relay
Managers.................................................................................................. 6721.0099
outputs
Column Identification System with Chip Cards, 5 pcs............................ 6710.1505
User Input/Display: LCD indicating system parameters, standby
Flow Manager with 2P-10P Valve initial operation and maintenance
The UltiMate 3000 Flow Manager FLM-3300 includes a GLP Features: Column Identification System, Automatic Equip-
two-position 10-port switching valve that enables preconcen- ment Qualification (AutoQ), System Wellness, and Qualification
tration, comprehensive 2-D LC (when used with an additional Monitoring with Chromeleon
FLM‑3300), and parallel LC (when used with an FLM-3200). Power Requirements: 162 VA, automatic voltage selection
The flow manager features the UltiFlow eluent delivery system
for nano, capillary, and micro flow rates, and is also available in Dimensions (h × w × d): 17 × 42 × 51 cm (6.7 × 16.5 × 20 in)
a biocompatible version (FLM-3300B).
• Column thermostatting from 5 to 70 ºC
• Short warm-up/cool-down times for fast temperature stabi- Hardware
lization
FLM-3300, Nano, 1X2P-10P Flow Manager with 2-Position 10-Port
• Column capacity of up to three microcolumns (max length: Switching Valve........................................................................................ 5720.0030
30 cm) FLM-3300, Cap, 1X2P-10P Flow Manager with 2-Position 10-Port
Switching Valve........................................................................................ 5720.0035
• UltiFlow technology for nano, capillary and micro flow
rates (50 nL/min–160 µL/min) FLM-3300, Micro, 1X2P-10P Flow Manager with 2-Position 10-Port
Switching Valve........................................................................................ 5720.0038
The UltiMate 3000 Flow Manager FLM-3300(B) can be easily FLM-3300B, Nano, 1X2P-10P Biocompatible Flow Manager with
converted between nano, capillary and micro flow rates by sim- 2-Position 10-Port Switching Valve......................................................... 5721.0030
ply exchanging the flow splitter cartridge. FLM-3300B, Cap 1X2P-10P Biocompatible Flow Manager with
2-Position 10-Port Switching Valve......................................................... 5721.0035
Note: For more details on Proteomics MDLC, comprehensive
2-D LC and Parallel LC, please refer to the LCi Solutions Accessories
section. Upgrade Kit to CAP LC, without Flow Cell, FLM Flow Managers.......... 6720.0095
Upgrade Kit to Micro LC, without Flow Cell, FLM Flow Managers........ 6720.0097
Key Specifications Upgrade kit to Nano LC, without Flow Cell, FLM Flow Managers......... 6720.0099
Upgrade Kit to CAP LC, Biocompatible, without Flow Cell, FLM Flow
Managers.................................................................................................. 6721.0095
Temperature Accuracy: ±0.5 ºC Upgrade Kit to Micro LC, Biocompatible, without Flow Cell, FLM Flow
Managers.................................................................................................. 6721.0097
Temperature Stability and Precision: ±0.1 ºC
Upgrade Kit to Nano LC, Biocompatible, without Flow Cell, FLM Flow
Heat-Up and Cool-Down Time: from 20 ºC to 50 ºC in less than Managers.................................................................................................. 6721.0099
25 min, from 50 ºC to 20 ºC in less than 35 min Column Identification System with Chip Cards, 5 pcs............................ 6710.1505
Switching Valves: one low-dispersion thermostatted 2-position

10-port micro valve
Column Capacity: Up to three columns, max. 30 cm length
46 www.dionex.com
LC Column Compartments
LC Column Compartments Related Literature
PDF documents under Literature on www.dionex.com

Product Data Sheets
UltiMate 3000 Thermostatted Column Compartment Series
RSLC Thermostatted Column

Compartment
The UltiMate 3000 Thermostatted Column Compartment

(TCC) offers precise temperature control under varying ambi-
ent conditions. Easily accessible valves extend flexibility and
performance options, and enable tandem and parallel operation,
on-line sample preparation, and multidimensional chromatog-
raphy as an integral part of the unique UltiMate 3000 x2 Dual
system capabilities.
• Column thermostatting for reliable LC at elevated, ambient,

and subambient temperatures
• Integrated switching valves for advanced column switching
techniques
The UltiMate 3000 Rapid Separation Thermostatted Column
• Freely-configurable and user-interchangeable high-pressure
Compartment TCC-3000RS operates at temperatures up to
switching valves
110 °C precisely and accurately. Advantages of high column
• Accomodation of up to six columns to facilitate automated temperatures are reduced system backpressures and typically
method development sharper peaks. Efficient precolumn heating and postcolumn
cooling technology ensures the best chromatography under
• Short equilibration times for temperature step gradients and these challenging conditions.
fast application switching
• Low-dispersion eluent preconditioner for better peak • Wide temperature range from 5–110 °C with excellent
shapes at elevated column temperature accuracy and precision
• Homogeneous temperature distribution via a fan-based, • Precise temperature control, even under varying ambient
forced-air design conditions
• AutoQ instrument qualification for simplified method docu- • Optional column switching valves in SST and PEEK up to
mentation and regulatory compliance 1034 bar (15,000 psi)
• Column identification system and comprehensive system • Fast and easy installation of precolumn heater and
wellness features postcolumn coolers
• Postcolumn cooling ensures lowest detector noise and drift
The Thermostatted Column Compartment meets the require- in ultrafast LC applications
ments of any laboratory with temperature-sensitive applications,
running methods at temperatures from 5 to 110 °C, with a need • Fast heat-up/cool down times
to increase laboratory performance and productivity using x2
• Increase your laboratory performance and productivity with
Dual system technology.
x2 dual technology
Note: For more information on x2 Dual systems and advanced • Column identification system for up to four columns
column switching techniques, please refer to the LCi Solutions
section.
www.dionex.com 47
LC Modules
The TCC-3000RS is highly flexible and supports up to 12 LC Ordering Information
columns (depending on column dimensions) to a length of up
to 30 cm. It can easily be upgraded with up to two integrated The TCC-3000RS and TCC-3000SD come without preinstalled
column switching valves providing access to advanced column column switching valves. For adding a switching valve to the
switching techniques. The TCC-3000RS meets the demands of TCC, a valve drive and a valve pod need to be ordered sepa-
ultrafast and conventional LC applications even at high column rately depending on left- or right-hand valve installation. A 2 µL
temperatures. pre-heater and a 2.2 µL post-column cooler insert are shipped
along with the TCC-3000RS.
Note: The TCC-3200RS is also available in a non-RSLC
version with a temperature range of 5–70 °C without post
Hardware
column cooling technology (TCC-3000SD).
TCC-3000RS Rapid Separation Thermostatted Column Compartment.. 5730.0000
Key Specifications
Compartment Temperature Range: TCC-3000RS: 5–110 °C or
18 °C below ambient, TCC-3000SD: 5–70 °C or 18 °C below Accessories
ambient
Valve Actuation Kit, Right Side, <1034 bar (15,000 psi), TCC-3000RS.. 6730.0001
Compartment Temperature Accuracy: ±0.5 °C Valve Actuation Kit, Left Side, <1034 bar (15,000 psi), TCC-3000RS..... 6730.0002
Compartment Temperature Stability and Precision: ±0.1 °C Valve Actuation Kit, Right Side, <413 bar (6000 psi), TCC-3000RS and
TCC-3000SD Series.................................................................................. 6730.0003
Compartment Heat-Up and Cool-Down Time: typically 12 min Valve Actuation Kit, Left Side, <413 bar (6000 psi), TCC-3000RS and
from 20 °C to 50 °C, typically 15 min from 50 °C to 20 °C TCC-3000SD Series.................................................................................. 6730.0004
Postcolumn Cooler Temperature Range (TCC-3200RS): 2-Position 6-Port Valve Pod, <1034 bar (15,000 psi), TCC-3000RS........ 6730.0006
5–110 °C (settable) 2-Position 6-Port Valve Pod, <413 bar (6000 psi), TCC-3x00 Series....... 6722.9013
2-Position 6-Port Valve Pod, Biocompatible, PEEK, <345 bar (5000 psi),
Postcolumn Cooling Capacity (TCC-3200RS):
TCC-3x00 Series....................................................................................... 6723.9013
min. 40 °C below column chamber temperature, at 100 °C with
2-Position 10-Port Valve Pod, Biocompatible, PEEK, <345 bar
water at 3 mL/min
(5000 psi), TCC-3x00 Series..................................................................... 6723.9023
Switching Valves (optional): one or two switching valves: 2-Position 10-Port Valve Pod, <345 bar (5000 psi), TCC-3x00 Series..... 6722.9023
2-position 6-port, 2-position 10-port, or 6-position 7-port valves 2-Position 7-Port Valve Pod, <413 bar (6000 psi), TCC-3x00 Series....... 6722.9035
Column Capacity: up to 12 columns (depending on column Precolumn Heater, 2 µL.......................................................................... 6722.00530
dimension), maximum column length 30 cm Precolumn Heater, 7 µL.......................................................................... 6722.00540
Precolumn Heater, 11 µL........................................................................ 6722.00550
PC Connection: all functions controlled via USB
Postcolumn Cooler Insert, 2 µL................................................................ 6730.0008
I/O Interfaces: two digital inputs, two relay outputs Column Identification System with Chip Cards, 5 pcs............................ 6710.1505
GLP Features: Column Identification System, Automatic Instru-
Power Requirements: 100–120 V, 60 Hz; 200–240 V, 50 Hz
Additional Specifications: please see data sheet
48 www.dionex.com
LC Column Compartments
TCC with Two Switching Valve Drives Key Specifications
Temperature Range: 5–85 °C or 18 °C below ambient
Temperature Accuracy: ±0.5 °C

Temperature Stability and Precision: ±0.1 °C
Heat-Up and Cool-Down Time: typically 15 min from 20 to
50 °C, typically 18 min from 50 to 20 °C
Switching Valve Pods (Optional): one or two thermostatted
switching valve pods: 2-position 6-port, 2-position 10-port, or
6-position 7-port valves
Column Capacity: up to 12 columns, maximum column length
30 cm
PC Connection: all functions controlled via USB
I/O Interfaces: two digital inputs, two relay outputs
The UltiMate 3000 Thermostatted Colum Compartment
TCC‑3200 offers precise temperature control up to 85 °C. It is
button, three LEDs for status monitoring, four function keys
very flexible in terms of switching valve compatibility—two-
position six-port or ten-port as well as six-position seven-port GLP Features: Column Identification System, Automatic
valves can be integrated into the thermostatted column chamber. Instrumentment Qualification (AutoQ), System Wellness, and
Qualification Monitoring with Chromeleon
• Wide temperature range from 5–85 °C with excellent
accuracy and precision Power Requirements: 100–120 V, 60 Hz; 200–240 V, 50 Hz
• Precise temperature control, even under varying ambient Dimensions (h × w × d): 18 × 42 × 51 cm (7.1 × 16.5 × 20 in.)
conditions Additional Specifications: please see data sheet
• Fast heat-up/cool-down times
• Optional valve pods provide access to x2 dual technology Ordering Information
• Increases laboratory productivity with advanced column The TCC-3200 comes with two integrated switching valve
switching techniques drives. Switching valve pods have to be ordered separately.
• Optional eluent preconditioners ensure optimal eluent ther-

mostatting for better resolution Hardware
• Fast and easy installation of eluent preconditioners with TCC-3200 Thermostatted Column Compartment with Two Built-In
volumes of 2, 7, or 11 µL Valve Drives (without Valve Pods)........................................................... 5722.0035
• Column identification system for up to four columns
The TCC-3200 supports LCi Solutions like parallel and tandem Accessories
analyses, online sample preparation, 2D-LC and application 2-Position 6-Port Valve Pod, <413 bar (6000 psi), TCC-3x00 Series....... 6722.9013
switching. Furthermore it facilitates method development with
2-Position 6-Port Valve Pod, Biocompatible, PEEK, <345 bar (5000 psi),
the LCi solution of Automated Method Scouting. TCC-3x00 Series....................................................................................... 6723.9013
2-Position 10-Port Valve Pod, Biocompatible, PEEK, <345 bar
Note: For more information on how you can benefit from (5000 psi), TCC-3x00 Series..................................................................... 6723.9023
advanced column switching techniques, please refer to the LCi 2-Position 10-Port Valve Pod, <345 bar (5000 psi), TCC-3x00 Series..... 6722.9023
solutions section.
2-Position 7-Port Valve Pod, <413 bar (6000 psi), TCC-3x00 Series....... 6722.9035
Precolumn Heater, 2 µL.......................................................................... 6722.00530
Precolumn Heater, 7 µL.......................................................................... 6722.00540
Precolumn Heater, 11 µL........................................................................ 6722.00550
Column Identification System with Chip Cards, 5 pcs............................ 6710.1505
www.dionex.com 49
LC Modules
LC Detectors RF 2000 Fluorescence Detector
RI-101 Refractive Index Detector
VWD-3000 Series Detectors

RSLC Diode Array Detector
A full range of detectors is available for the UltiMate 3000 LC

and LC-MS systems. Choose from UV, RI, Fluorescence, or
Mass Spectrometer detectors according to your LC application
needs. All data generated can be easily processed and reported
using the powerful tools in the Chromeleon Chromatography
The UltiMate 3000 Rapid Separation Diode Array Detector
Management System.
DAD-3000RS is the detector of choice if you need maximum
• The UV/Vis Diode Array Detectors enable peak character- flexibility in your laboratory. It offers reliable UV-Vis detection
ization and ultrafast LC. for both conventional and Rapid Separation LC applications
with full wavelength spectra acquisition with 1 nm pixel resolu-
• The UV/Vis Variable Wavelength Detectors offer high flex- tion and data collection rates up to 100 Hz.
ibility for any laboratory.
• Low noise (typically <±7 µAU) and drift (<0.5 mAU/h) for
• The Refractive Index Detector supports detection of lowest LODs and LOQs
analytes with limited UV absorption.
• Data collection rates of up to 100 Hz allow detection and
• The RF 2000 Fluorescence Detector provides sensitive characterization of even the sharpest peaks
detection of trace-level analytes.
• Full UV-Vis spectra data acquisition even for 10 peaks
• All detectors support easy data processing and reporting separated in 10 s
with Chromeleon, even for mass spectrometry data.
• Stainless steel and PEEK flow cells provide maximum ap-
High quality detector components, intelligent mechanical and plication flexibility
electronic detector design, and rigorous quality assurance test-
ing guarantee durable and reliable operation. AutoQ equipment • Front access to prealigned cells and lamps simplifies rou-
qualification tests and predictive performance indicators sim- tine maintenance
plify installation, qualification, and performance verification. • ID chips for tracking of lamp and cell parameters in audit
trails
Note: For further information on mass spectrometers and
related devices please refer to the MS Modules section. The Ultimate 3000 Rapid Separation Diode Array Detector
DAD-3000RS features eight channels of single-wavelength
data acquisition, a 1024 element diode array and excellent noise
Related Literature and drift performance close to forward-optic detectors . The
comprehensive set of 3-D data tools in the Chromeleon Chro-
For detailed specifications and applications, see the following matography Management Software makes it easy to process and
PDF documents under Literature on www.dionex.com report your data.
Note: The DAD-3000RS is also available in a standard

Product Data Sheets version (DAD-3000) with a data rate of up to 20 Hz for
UltiMate 3000 Diode Array Detector Data Sheet conventional LC methods.
50 www.dionex.com
LC Detectors
Key Specifications Accessories
Detection Type: Single-beam, reverse optics design with con- Deuterium Lamp, VWD-3x00, DAD-3000, and MWD-3000 Series........ 6074.1110
cave holographic grating Tungsten Lamp, VWD-3x00, DAD-3000, and MWD-3000 Series.......... 6074.2000

Maximum Data Collection Rate: DAD-3000RS: 100 Hz, DAD-
3000: 20 Hz
Wavelength Range: 190–800 nm DAD‑3000 and MWD-3000 Series.......................................................... 6082.0300
Note: For more information on additional flow cells, please
Noise: <±10 µAU at 254 nm, response time 2 s (according to
contact your local sales representative.
ASTM time constant ~1 s), 4 nm bandwidth, water at
1.0 mL/min
Drift: <1 mAU/h (typically <0.5 mAU/h) at 254 nm and 520 nm RSLC Multiple Wavelength Detector
with water at 1.0 mL/min
Linearity: <3% RSD and corr. coeff. >0.9995 up to 1.5 AU,
typically <2.5% RSD and corr. coeff. >0.999 up to 1.8 AU
Light Source: deuterium lamp, tungsten lamp, temperature
control for both lamps
Wavelength Accuracy: ±1.0 nm, self-calibration with D-alpha
line, verification with holmium oxide filter
Pixel Resolution: <1 nm
PC Connection: All functions controllable via USB 2.0;
integrated USB hub with three USB 2.0 ports
GLP Features: Automatic Equipment Qualification (AutoQ),
System Wellness, and Qualification Monitoring with Chrome-
leon, lamp and cell ID chips
The UltiMate 3000 Rapid Separation Multiple Wavelength
Analog Outputs: two, available as an option via DAC plug-in Detector MWD-3000RS allows you to collect UV/Vis data from
module, 0–1 V (full range) and 0–10 V (full range with adjust- up to 8 different wavelengths between 190 and 800 nm simulta-
able mAU ranges) neously. It offers reliable UV-Vis detection for conventional and
User Input/Display: LCD indicating system parameters, standby Rapid Separation LC application at data collection rates up to
button, three LEDs for status monitoring, four function keys for 100 Hz per channel.
• Low noise (typically <±7 µAU) and drift (<0.5 mAU/h) for
Power Requirements: 85–260 V ac, 50/60 Hz, max. 150 W lowest LODs and LOQs
Dimensions (h × w × d): 16 × 42 × 51 cm (6.3 × 16.5 × 20 in.) • Data collection rates of up to 100 Hz allow detection of
even the sharpest peaks
Ordering Information • UV-Vis detection of 10 peaks separated in 10 s is possible
The Diode Array Detector DAD-3000RS and DAD-3000 are • Stainless steel and PEEK flow cells provide maximum
shipped without flow cells. Flow cells must be ordered sepa- application flexibility
rately and according to your LC application requirements.
• Front access to prealigned cells and lamps simplifies
routine maintenance
Hardware • ID chips for tracking of lamp and cell parameters in audit
DAD-3000RS Rapid Separation Diode Array Detector (without Flow trails
Cell)........................................................................................................... 5082.0020
DAD-3000 Diode Array Detector (without Flow Cell)............................. 5082.0010 The UltiMate 3000 Rapid Separation Multiple Wavelength
Detector MWD-3000RS provides high flexibility to your labo-
ratory working in routine analyses and method development.
Besides from UV/VIS spectra acquisition it combines all the
advantages of the DAD-3000RS like identical noise and drift
characteristics and high wavelength accuracy and precision.
www.dionex.com 51
LC Modules
Note: The MWD-3000RS is also available in a standard Hardware
version (MWD-3000) with a data rate of up to 20 Hz for
MWD-3000RS Rapid Separation Multiple Wavelength Detector
conventional LC methods. (without Flow Cell)................................................................................... 5082.0040
MWD-3000 Multiple Wavelength Detector (without Flow Cell)........... 5082.0030
Key Specifications
Accessories
Detection Type: Single-beam, reverse optics design with con-
cave holographic grating Deuterium Lamp, VWD-3x00, DAD-3000, and MWD-3000 Series........ 6074.1110
Tungsten Lamp, VWD-3x00, DAD-3000, and MWD-3000 Series.......... 6074.2000
Maximum Data Collection Rate: MWD-3000RS: 100 Hz,
MWD-3000: 20 Hz
Wavelength Range: 190–800 nm Flow Cell, Semi-Micro, SST, 2.5 µL Volume, 7 mm Pathlength,
Noise: <±10 µAU at 254 nm, response time 2 s (according to
ASTM time constant ~1 s), 4 nm bandwidth, water at Note: For more information on additional flow cells, please
1.0 mL/min contact your local sales representative.
Drift: <1 mAU/h (typically <0.5 mAU/h) at 254 nm and 520 nm

with water at 1.0 mL/min RSLC Variable Wavelength Detector
Linearity: <3% RSD and corr. coeff. >0.9995 up to 1.5 AU,
typically <2.5% RSD and corr. coeff. >0.999 up to 1 .8 AU
Light Source: deuterium lamp, tungsten lamp, temperature
control for both lamps
Wavelength Accuracy: ±1.0 nm, self-calibration with D-alpha
line, verification with holmium oxide filter
Pixel Resolution: <1 nm
PC Connection: All functions controllable via USB 2.0; inte-
grated USB hub with three USB 2.0 ports
GLP Features: Automatic Equipment Qualification (AutoQ),
Analog Outputs: two, available as an option via DAC plug-in
The UltiMate 3000 Rapid Separation Variable Wavelength De-
module, 0-1V (full range) and 0–10 V (full range with adjust-
tector VWD-3400RS offers high sensitivity and excellent noise
able mAU ranges)
and drift performance for reliable UV-Vis detection in RSLC
User Input/Display: LCD indicating system parameters, standby applications with data collection rates up to 100 Hz. The detec-
button, three LEDs for status monitoring, four function keys for tor is designed to meet the demanding requirements of today’s
initial operation and maintenance laboratories.
Power Requirements: 85–260 V ac, 50/60 Hz, max. 150 W • Low noise (typically <±2.5 µAU) and drift (<0.1 mAU/h)
Dimensions (h × w × d): 16 × 42 × 51 cm (6.3 × 16.3 × 20 in.) for ultralow LODs and LOQs
• Data collection rates of up to 100 Hz allow detection of
Ordering Information even the sharpest peaks
The Multiple Wavelength Detector MWD-3000RS and MWD- • Linear range of up to 2.5 AU supports the analysis of a
3000 are shipped without flow cells. Flow cells must be ordered wide analyte concentration range
separately and according to your LC application requirements. • For use with LC applications from nano- to semipreparative
LC
• Spectrum scanning functionality for analyte characteriza-
tion
• Predictive performance technology continually monitors
system performance
52 www.dionex.com
LC Detectors
The VWD-3400RS Rapid Separation Variable Wavelength Hardware
Detector is ideal for RSLC applications in single wavelength
VWD-3400RS Rapid Separation Variable Wavelength Detector
mode. For conventional LC applications the detector allows data (without Flow Cell)................................................................................... 5074.0010
acquisition of up to 4 UV-Vis channels up to 5 Hz. Its wave-

length scanning feature allows for analyte characterization.
Accessories
Deuterium Lamp, VWD-3x00, DAD-3000, and MWD-3000 Series........ 6074.1110
Key Specifications
Detection Type: Double beam forward optics design (mono- Flow Cell, Analytical, SST, 11 µL Volume, 10 mm Pathlength, VWD-
chromator) 3x00 Series............................................................................................... 6074.0250
Flow Cell, Analytical, PEEK, 11 µL Volume, 10 mm Pathlength, VWD-
Maximum Data Collection Rate: single wavelength: 100 Hz, 3x00 Series............................................................................................... 6074.0200
multiple wavelength: 5 Hz, depending on number of wave-
Flow Cell, Semi-Micro, SST, 2.5 µL Volume, 7 mm Pathlength, VWD-
lengths and wavelength distance
3x00 Series............................................................................................... 6074.0360
Wavelength Range: 190–900 nm Flow Cell, Semi-Micro, PEEK, 2.5 µL Volume, 7 mm Pathlength, VWD-
3x00 Series............................................................................................... 6074.0300
Noise: single wavelength: <±3.5 µAU, multiple wavelength:
UZ-View Flow Cell, Nano, 3 nL Volume, 10 mm Pathlength, VWD-
<±25 µAU at 254 nm, time constant 1 s, dry analytical flow cell 3x00 Series............................................................................................... 6074.0270
Drift: <0.1 mAU/hr at 254 nm, dry analytical flow cell, only UZ-View Flow Cell, Capillary, 45 nL Volume, 10 mm Pathlength, VWD-
deuterium lamp turned on 3x00 Series............................................................................................... 6074.0280
UZ-View Flow Cell, Micro, 180 nL Volume, 10 mm Pathlength, VWD-
Linearity: <5% RSD at 2.5 AU and 272 nm based on ASTM 3x00 Series............................................................................................... 6074.0290
Light Source: deuterium lamp, tungsten lamp, temperature Flow Cell, Semipreparative, PEEK, 0.7 µL Volume, 0.4 mm Pathlength,
control for both lamps VWD-3x00 Series..................................................................................... 6074.0320
Analog Output Board, Max. 2 Channels, VWD-3x00 Series................... 6074.0305
Wavelength Accuracy: ±1 nm, self calibration with D-alpha line,
verification with holmium oxide filter
Optical Bandwidth: 6 nm at 254 nm
Spectrum Scanning: yes, four scan speeds up to 33 nm/s
PC Connection: all functions controllable through USB
GLP features: Automatic Equipment Qualification (AutoQ),
module, 0–1 V (full range) and 0–10 V (full range with adjust-
able mAU ranges)
The Variable Wavelength Detector VWD-3400RS is shipped

without flow cells, which must be ordered separately based on
your LC application. For multiple wavelength detection in rou-
tine analysis, consider the multiple wavelength detector MWD-
3000(RS) or diode array detector DAD-3000(RS).
www.dionex.com 53
LC Modules
Variable Wavelength Detector Linearity: <5% RSD at 2.5 AU and 272 nm based on ASTM
Light Source: deuterium lamp (optional tungsten lamp), tem-
perature control for both lamps
Wavelength Accuracy: ±1 nm, self calibration with D-alpha line,

verification with holmium oxide filter
Optical Bandwidth: 6 nm at 254 nm
Spectrum Scanning: yes, four scan speeds up to 33 nm/s
PC Connection: all functions controllable through USB
GLP features: Automatic Equipment Qualification (AutoQ),
module, 0–1 V (full range) and 0–10 V (full range with adjust-
able mAU ranges)
The VWD-3100 Variable Wavelength Detector with single
wavelength capability is the detector of choice for routine chro- User Input/Display: LCD indicating system parameters, standby
matography applications. It offers data collection rates of up to button, three LEDs for status monitoring, four function keys for
20 Hz, high sensitivity, ultralow noise and drift, and a wave- initial operation and maintenance
length range of 190–900 nm.
• Low noise (typically <±2.5 µAU) and drift (<0.1 mAU/h)
ensure the lowest LODs and LOQs
• Linear range of up to 2.5 AU supports the analysis of a
wide analyte concentration range The Variable Wavelength Detector VWD-3100 is shipped with
deuterium lamp only. Tungsten lamp and flow cells need to be
• For use with LC applications from nano- to semipreparative ordered separately.
LC
• Spectrum scanning functionality for analyte characteriza-
Hardware
tion
• Predictive performance technology continually monitors Flow Cell).................................................................................................. 5074.0005
system performance
• Priced for laboratories on a limited budget Accessories
Deuterium Lamp, VWD-3x00, DAD-3000, and MWD-3000 Series........ 6074.1110
The UltiMate 3000 Variable Wavelength Detector VWD-3100 is
ideally priced for laboratories on a limited budget. Its wave-
length scanning feature allows for analyte characterization. Flow Cell, Analytical, SST, 11 µL Volume, 10 mm Pathlength, VWD-
3x00 Series............................................................................................... 6074.0250
Flow Cell, Analytical, PEEK, 11 µL Volume, 10 mm Pathlength, VWD-
Key Specifications 3x00 Series............................................................................................... 6074.0200
Flow Cell, Semi-Micro, SST, 2.5 µL Volume, 7 mm Pathlength, VWD-
Detection Type: double beam forward optics design (monochro-
3x00 Series............................................................................................... 6074.0360
mator)
Flow Cell, Semi-Micro, PEEK, 2.5 µL Volume, 7 mm Pathlength, VWD-
Light Source: deuterium lamp, (optional: tungsten lamp), tem- 3x00 Series............................................................................................... 6074.0300
perature control for both lamps UZ-View Flow Cell, Nano, 3 nL Volume, 10 mm Pathlength, VWD-
3x00 Series............................................................................................... 6074.0270
Wavelength Range: 190–900 nm, the tungsten lamp is recom-
UZ-View Flow Cell, Capillary, 45 nL Volume, 10 mm Pathlength, VWD-
mended for wavelengths >600 nm
3x00 Series............................................................................................... 6074.0280
Noise: <±3.5 µAU (typically <±2.5 µAU) at 254 nm, time con- UZ-View Flow Cell, Micro, 180 nL Volume, 10 mm Pathlength, VWD-
stant 1 s (according to ASTM), dry analytical flow cell 3x00 Series............................................................................................... 6074.0290
Flow Cell, Semipreparative, PEEK, 0.7 µL Volume, 0.4 mm Pathlength,
Drift: <0.1 mAU/hr at 254 nm, dry analytical flow cell, only
VWD-3x00 Series..................................................................................... 6074.0320
deuterium lamp turned on
Analog Output Board, Max. 2 Channels, VWD-3x00 Series................... 6074.0305
54 www.dionex.com
LC Detectors
Fluorescence Detector Outputs: digital data output via RS-232; recorder: 1 mV, 10 mV;
integrator: 1 V/FS.
Inputs: contact signal input (START input, STOP input)

Flow Cell: 12-µL cell volume, 20 bar (290 psi) maximum cell
pressure
PC Communication: through RS-232C, fully controllable with
Chromeleon Chromatography Management Software
Time-Programmed Functions: excitation and emission wave-
lengths (up to 32 steps), response switching, wavelength scan,
and autozero
Power Requirements: 100–240 V, 50/60 Hz, 350 VA
Dimensions (h × w × d): 20.5 × 26 × 52 cm (8.1 × 10.2 × 20.5
in.)
The Fluorescence Detector RF 2000 is the perfect choice for Ordering Information
the selective detection of nalytes such as polycyclic aromatic
hydrocarbons (PAH), aflatoxins, carbamates, vitamins, or amino
acids. With a signal-to-noise ratio better than 300:1 for the Hardware
Raman line of water, the detector can reach very low limits of RF 2000 Spectrofluorometric Detector.................................................... 5057.0020
detection.
Accessories
• Trace-level analyte detection
XenonLamp, 150 W, RF 2000 Detector.................................................... 5057.1000
• Flexibility and easy method development
Photomultiplier 200-900 nm, RF 2000 Detector...............................706.200.75021
• Reliability: wavelength accuracy and reproducibility Flow Cell, Analytical, 12 µL, RF 2000 Detector............................706.228.3384391
Flow Cell, Micro, 2 µL, PEEK, RF 2000 Detector..........................706.228.34197.93
• Time-saving automated validation
The independent monochromators of the RF 2000 provide the

highest reproducibility of excitation and emission wavelengths
(±0.2 nm), thereby facilitating the adaptation of methods to the
optimum detection condition for individual components. Wave-
length range can be extended up to 900 nm.
Key Specifications
Light Source: high-precision xenon lamp, 150 W
Optical System: concave, blazed holographic grating monochro-
mators for both excitation and emission beams
Excitation/Emission Wavelength Range: 200–650 nm (option-
ally 200–900 nm)
Spectral Bandwidth: 15 nm for both excitation and emission
Wavelength Accuracy/Repeatability: ±2 nm/±0.2 nm
Wavelength Moving Speed: ≈15,000 nm/min
Wavelength Scanning Speed: four settable scan speeds: super-
approx. 3,000 nm/min, fast-approx. 600 nm/min, medium-
approx. 120 nm/min and slow-approx. 24 nm/min
Sensitivity: S/N ratio >300 for the Raman line of water (350 nm
excitation wavelength, 1.5-s time constant)
www.dionex.com 55
LC Modules
Refractive Index Detector Temperature Control: 30–50 °C in 1 °C steps
Analog Outputs: Integrator, 0–1 V; Recorder, 0–10 mV
Contact Closure Inputs: Autozero, Marker, Polarity, Purge on/
off
Contact Closure Outputs: Ready, Solvent leak, Error
PC Communication: RS-232
Power Consumption: 150 VA Max.
Dimensions (h × w × d): 26 × 20 × 40 cm (10.1 × 7.8 × 15.6
in.)
Hardware
The Refractive Index Detector RI-101 is the detector of choice RI-101 Refractive Index Detector including flow cell (8 µL).................... 5060.0030
if you need to detect analytes with limited or no UV absorption
such as alcohols, sugars, carbohydrates, fatty acids, and poly-
mers. Good trace detection performance is assured through a
noise below 2.5 nRIU. An optimized temperature control system
ensures fast baseline stabilization after system start-up.
• Innovative and sophisticated temperature control for fast

startup in less than 40 min
• Built-in startup sequence with automated purging and con-
trol of baseline stability and noise
• AutoQ validation functions for time savings and easy
validation
• Operation with Chromeleon software for maximum pro-
ductivity
The Refractive Index Detector provides ease of use with a start-

up sequence that automates purging, equilibration, autozero, and
the control of baseline stability and noise. In addition, a full-
color, liquid-crystal display provides fast access to instrument
settings and performance parameters, and shows an on-line
signal.
Key Specifications
Measuring Method: deflection method
Refractive Index Range: 1.00–1.75°
Measuring Range: 1/4–512 µRIU
Linearity Range: 600 µRIU
Noise Level: less than 2.5 nRIU, response: 1.5 s
Flow Cell: 8 µL; 0.05 MPa (7.5 psi) pressure maximum
Dead Volume: less than 600 µL
Maximum Flow Rate: 10 mL/min with water
56 www.dionex.com
Fraction Collection
Fraction Collection WPS Microfraction Collection Option
Dionex provides the right solution for your fraction collection

needs with easy-to-use fraction collection devices for a wide va-

riety of LC applications, such as multidimensional LC, MALDI
target spotting for MALDI-MS analyses, as well as analytical or
semipreparative LC separations.
• WPS-3000PL microfraction collection option for automat-

ed off-line multidimensional LC
• Probot MALDI spotter for on-line µL- to nL-fraction col-
lection
• Foxy Jr. for fraction collection at analytical and semiprepar-
ative flow rates
• Flexibility with comprehensive Chromeleon fraction collec-
tion capabilities
The Microfraction Collection (µFC) option for the WPS-
3000(T)PL Nano/Cap Autosampler enables fully-automated
Related Literature injection, fractionation, and re-injection on a single, integrated
system. Taking full advantage of the three well plate capacity,
For detailed specifications and applications, see the following the system is capable of fractionating and reinjecting more than
PDF documents under Literature on www.dionex.com. 1000 samples without the need for any manual handling.
• Highest flexibility with respect to column dimensions and

Product Brochures
mobile phase selections
Probot Microfraction Collector Brochure
• Easy method development
Product Data Sheets • Dedicated optimization of each separation dimension
UltiMate 3000 Autosampler Series • Multiple analyses of the fractionated sample, even with
different methods
Application Notes
The µFC option can be used only with the WPS-3000(T)PL and
AN 530: Proteome Analysis Involving Off-Line 2-D LC of In-
WPS-3000TBPL autosamplers. This option used with the Ul-
tact Proteins, Proteolytic Digestion, and Capillary RP-LC-MS/
tiMate 3000 Proteomics MDLC system offers fully automated
MS Analysis Using Monolithic PS-DVB Columns
off-line multidimensional (MD) LC for a variety of methods and
AN 526: PS-DVB Monolithic Columns Applied in an Off-Line flow rate ranges.
2-D LC/ESI-MS Bottom-Up Study for the Identification of
Platelet Proteins Note: For more details on the µFC option and the UltiMate
3000 Proteomics MDLC system, please refer to the LCi
Solutions section.
Hardware parts listed below are needed to upgrade the WPS-

3000(T)PL Autosamplers in exisiting UltiMate 3000 Nano/
Capillary/Micro system configurations. For information on frac-
tion collection control and automation features in Chromeleon
Chromatography Software, please contact your local Dionex
representative.
Hardware
Micro Fraction Collection Option, WPS-3000PL..................................... 6820.0051
WPS-3000PL Micro Fraction Collection Option, Biocompatible............. 6821.0051
www.dionex.com 57
LC Modules
Probot MALDI Spotter Precision of Table Positioning: better than 20 µm
Spotting Frequency: user-selectable down to 5 s with minimal
volumes of 5 nL
Lifting Force: >2 kp

Carryover: in pipetting mode, less than 0.02% (with additional
needle wash)
Dimensions (h × w × d): 30 × 48 × 50 cm (12 × 19 × 20 in.)
Software: fully controlled by µCarrier Software, supported by
Chromeleon.
Collection Modes: time-based collection and peak collection
Hardware
The Probot MALDI spotter is designed for on-line collection
of µL- and nL-fractions eluting from nano and capillary LC Probot, Microfraction Collector with Dosage Unit.................................. 5713.0200
systems on different types of sample carriers such as MALDI
targets, PVDF membranes, 96, 384, or 1536 well plates, or Accessories
microchip structures. The X,Y,Z-moving table and fixed needle
Probot Table Adapter for 6 × ABI 4700 Proteomics Analyzer................. 5713.6402
design allow nL-spotting with excellent precision and minimal
Probot Table Adapter for 2 × ABI 4800.................................................... 6713.0022
dead volume.
Probot Table Adapter for 2 × Bruker MALDI Target (Micro Titer Plate
• Robotic system for MALDI spotting (and fraction collec- type).......................................................................................................... 5713.6307
tion) Probot Table Adapter for 6 × Micromass Rectangular Targets.............. 5713.6328
Probot Table Adapter for 2 × Shimadzu/Kratos Axima QIT Targets....... 5713.6319
• Supports MALDI targets and sample carriers from every
vendor Probot Table Adapter for 2 × Thermo Scientic vMaldi............................ 6713.0025
Probot Table Adapter with Adjustable Strips.......................................... 5713.6338
• Small nL-volumes collection with zero chromatographic
Probot Table Adapter for 2 × Micro Titer Plates...................................... 5713.6348
dispersion maintaining LC resolution
• Two well plate (or six AB4700) capacity for collection of
more than 4000 spots
• Integrated reagent (matrix) addition for easy MALDI-
spotting
• Dual-collection mode for fraction splitting over two differ-
ent sample carriers
Typical Probot applications include on-line MALDI target

preparation or microfraction collection, e.g., collection of
proteins from first-dimension separations. Due to its accuracy
and flexibility, the Probot is the ideal nano-LC/MALDI-MS
interface, integrating the high-sensitivity separation power of
nano-LC and high resolving power of MALDI-MS.
Key Specifications
Table Dimensions (w × d): 210 × 135 mm (8.27 × 5.31 in.)
Table Movement: X = 195 mm, speed 0.250–20 mm/s;
Y = 130 mm, speed 0.125–10 mm/s; Z = 95 mm, speed
0.125–10 mm/s
58 www.dionex.com
Fraction Collection
Foxy Jr. Fraction Collector Ordering Information
The Foxy Jr. Fraction Collector is a rugged and easy-to-use LC Hardware

fraction collector ideally suited for routine usage at analytical

flow rates and flow rates up to 100 mL/min. Its small footprint Foxy Jr Fraction Collector with Diverter Valve........................................ 5701.2000
saves benchspace. Although small in size, it is high in versatil-
ity, offering 11 different racks and collection vessel types from Accessories
MiniVials to Prep funnels.
Additional Rack for Foxy Jr. Fraction Collector, 12 mm, 144.................. 5701.2020
• For routine usage and flow rates up to 100 mL/min Additional Rack for Foxy Jr. Fraction Collector, two 96 wellplates........ 5701.2021
Additional Rack for Foxy Jr. Fraction Collector, 18 mm, 72.................... 5701.2024
• Adaptable instrument height for accommodation of up to
Additional Rack for Foxy Jr. Fraction Collector, 16 mm, 100.................. 5701.2022
150 mm tall collection vessels
Additional Rack for Foxy Jr. Fraction Collector, 1.5 mL, 60..................... 5701.2023
• Fast and easy setup and operation
• Fast transit times between tubes and flow diversion options
prevent sample loss
• Large drip pan with drain for even more security
Use the Foxy Jr. Fraction Collector as a stand-alone instrument,

or in combination with Chromeleon to further simplify setup
and routine usage. With the fraction collection capabilities of
Chromeleon, you will be able to collect peaks by time, volume,
signal threshold, signal slope, or using more than one detector
channel.
Key Specifications
Capacity: 60, 1.5 mL microcentrifuge tubes; 144, 12- or 13 mm
tubes; 100, 16 mm tubes; or 72, 18 mm tubes/MiniVials or
1.5 mL microcentrifuge tubes
Capacity (continued): 2, 96-well microplates; 36, 25 mm tubes,
28 mm scint. vials, 29 mm diameter tapered centrifuge tubes or
prep funnels; or 9, 150 mL beakers
Collection Basis: Time: 1 s to 99 h: 59 min: 59 s, Drops:
1–999,999 drops, Volume: 1–999,999 volume units
Antidrip Delay: 0.8 s with valve installed or pump stop between
tubes is enabled
Tube Change Time: 260–460 ms tube center to tube center,
depending on rack type and acceleration setting
Maximum drop speed: 5.5 drops/s
LC Interfacing: Serial (RS 232): analog 10 mV, 100 mV, and 1
V detector input for peak collection, contact closure I/O, time-
based valve/accessory control
Event mark output: open collector, 100 ms duration
Wetted Components: Halar drop former; polypropylene waste
funnel and drip pan
Operating Conditions: temperature: 5–40 ºC (41–104 ºF); air
humidity: 95% RH 0–20 ºC; 90%
Dimensions (h × w × d): 27 × 28 × 31 cm
(10.6 × 11.0 × 12.2 in.)
www.dionex.com 59
60
LC Modules
www.dionex.com
Ion Chromatography Instruments
RFIC Solutions 62 IC & RFIC Modules 73
Reagent-Free IC with Eluent Generation............ 62 Eluent Generation................................................. 74

Reagent-Free IC with Eluent Regeneration........ 62 ICS-3000 EG Eluent Generator.......................................... 74
RFC-30 Reagent-Free Controller...................................... 75
RFC-10 Reagent-Free Controller...................................... 75
IC & RFIC Systems 63 IC & RFIC Pumps................................................... 76

ICS-3000 DP Dual Pumps................................................... 76
ICS-3000................................................................. 64 ICS-3000 SP Single Pumps................................................ 76
Innovative Reagent-Free and Dual IC............................. 64 EO Eluent Organizer and Accessories............................ 77
Related Literature................................................................ 65 AXP Auxiliary Pump............................................................ 77
IC & RFIC Injectors & Autosamplers................... 79
ICS-2000................................................................. 66 Related Literature................................................................ 79
Related Literature................................................................ 67 AS Autosampler................................................................... 79
Ordering Information.......................................................... 67 AS40 Autosampler.............................................................. 80
AS-HV Autosampler............................................................ 81
ICS-1500................................................................. 68 MX Six-Port Injection Valve.............................................. 82
Eluent Regeneration........................................................... 68
Related Literature................................................................ 68 IC & RFIC Column Compartments....................... 83
Ordering Information.......................................................... 68 ICS-3000 Thermal Compartment...................................... 83
Detector/Chromatography Compartment...................... 84
ICS-1000................................................................. 69 ICS-1000/1500/2000 Column Heater................................. 85
Eluent Regeneration........................................................... 69
Related Literature................................................................ 69 IC & RFIC Detectors............................................... 86
Ordering Information.......................................................... 69 ICS-3000 CD Conductivity Detector................................. 86
ICS-3000 ED Electrochemical Detector.......................... 87
ICS-900................................................................... 70 ICS Series Variable Wavelength Detector..................... 88
Related Literature................................................................ 71 ICS Series Photodiode Array Detector........................... 89
Postcolumn Reaction Systems............................ 90
PC10 Postcolumn Delivery System.................................. 90
www.dionex.com 61
RFIC Solutions
RFIC Solutions
Reagent-Free IC with Reagent-Free IC with

Eluent Generation Eluent Regeneration
Always On, Always Ready
Outstanding consistency for gradient and isocratic

applications RFIC-ER™ systems (available option for ICS-1000, -1500,
Reagent-Free™ IC (RFIC™) systems with eluent generation -2000, and -3000 systems) are designed for routine isocratic
include the ICS-3000 and ICS-2000. These RFIC‑EG™ separations. The systems use the Self-Regenerating Suppressor
systems combine automated eluent generators and Self- and patented trap and purification columns to electrolytically
Regenerating Suppressors® to electrolytically create the eluents regenerate suppressed eluent. A single 4 L bottle of eluent can
and regenerants needed for IC applications. Plumb in a clean be recirculated for up to 4 weeks.
source of deionized water, and the RFIC-EG system takes care
of the rest. When left on, an RFIC-ER system remains equilibrated and
calibrated between eluent changes. Time that would have been
used maintaining the system can now be used for running ad-
ditional samples, increasing productivity.
Pump
Pump
Purification Column
Waste Eluent
Generator Injector
Injector Guard Column
Guard Column ER
Controller
Analytical
Column
Analytical
Column
Chromeleon
Workstation
Chromeleon Electrolytic
Workstation Suppressor
Electrolytic
CRD
Suppressor
Analyte Trap
Conductivity
Column Conductivity Detector
Conductivity Cell
Conductivity Detector
Cell
RFIC-EG System RFIC-ER System

Eluent generators increase accuracy and reproducibility and simplify Eluent regeneration saves time and reduces cost for frequent-use iso-
operation. Eluent generation is an important reason why RFIC-EG cratic analyses. The always on, always ready RFIC-ER system is ideal
systems are superseding traditional IC systems. for municipalities and contract labs that monitor water quality.
62 www.dionex.com
IC & RFIC Systems
A complete range of ion chromatography solutions
for all customer performance and price requirements
For ion analysis, nothing compares to a Dionex ion chromatog-
raphy system. Whether you have just a few samples or a heavy

workload, whether your analytical task is simple or challenging,
we have a solution to match your needs and budget. And with
your IC purchase, you get more than just an instrument—you
get a complete solution based on the modern technology and
ICS-3000: From routine, dedicated systems to high-throughput
world-class support of Dionex, the leader in IC for over 25
dual RFIC, the ICS-3000 is truly designed for versatility and
years.
productivity.
• ICS-3000 Award-Winning Reagent-Free Ion Chromatogra- ICS-2000: The first integrated RFIC-EG system for electrolyti-
phy system cally generated isocratic and gradient separations with conduc-
• ICS-2000 Award-Winning Integrated Reagent-Free IC tivity detection.
system ICS-1500: With dual-piston pumping and electrolytic suppres-
• ICS-1500 Standard Integrated IC system sion, the ICS-1500 combines high sensitivity with set-and-forget
convenience. Now featuring RFIC-ER.
• ICS-1000 Basic Integrated IC system
ICS-1000: With dual-piston pumping and electrolytic suppres-
• ICS-900 Starter Line IC system sion. Now available with optional RFIC-ER.
Ranging from the ICS-900 to the ICS-3000, Dionex ion ICS-900: Can routinely analyze multiple anions and cations in
chromatography systems cover the entire range of IC needs 10–15 min—fully automated with DCR Displacement Chemi-
and budgets. Dionex systems come with superior support and cal Regeneration.
service worldwide.
Note: See also the Supplies and Accessories section for RFIC-
EG and RFIC-ER accessory ordering information.
www.dionex.com 63
IC & RFIC Systems
ICS-3000
Award-winning, modular ICS-3000 RFIC-EG system
The ICS-3000 Reagent-Free IC system with Eluent Generation
is the most innovative ion chromatography system on the market
today. From new autosampling technologies, including well
plate capabilities, to dual electrochemical capabilities and opti-
cal detection, the ICS-3000 breaks new ground as the innovation

leader. The ICS-3000 allows chemists to save time, labor, and
operating cost while increasing the productivity and reproduc-
ibility of ion analysis for any application.
• Autosamplers to cover automation needs from simple injec-

tions to complex sample prep
• ICS-3000 EG Eluent Generator for the benefits of Reagent- The ICS-3000 has won five awards including an Editors' Choice Silver
Free IC for best new products at Pittcon '05 and two Golds from Instrument
Business Outlook.
• ICS-3000 DC Detector/Chromatography module, a high
performance environmental chamber
• ICS-3000 TC Thermal Compartment for precise tempera-
ture control from 5 to 85 ºC
• Flexible, modular design
• Wide range of detectors: conductivity, electrochemical,
UV/VIS, Photodiode Array, MS etc.
• Basic IC configurations for routine, dedicated analysis
• Dual-RFIC system configurations for high-throughput and
complex applications
The wide variety of ICS-3000 modules enables you to con-

figure an ion chromatography system designed specifically to
provide the solutions you need. And with your IC purchase, you
get more than just an instrument—you get a complete solution
based on the modern technology and the world-class support of Innovative Reagent-Free and Dual IC
Dionex, the leader in IC for more than 25 years.
With the introduction of eluent generation technology, Di-
onex has simplified ion chromatography while simultaneously
Significant performance enhancements make the ICS-3000 the
increasing the power and reproducibility of ion analysis. RFIC
most reproducible, stable, and sensitive ion chromatography
redefines the way ion chromatography is performed. The ICS-
system available today. Dramatic improvements in flow rate
3000 offers both RFIC-EG (standard) and RFIC-ER (optional)
accuracy, eluent generator electronics stability, and conductivity
capabilities.
cell temperature control increase baseline stability and enhance
sensitivity.
The system can be easily upgraded from a single- to a dual-
system configuration—boosting productivity without taking
Note: See the IC Modules section for equipment
up more valuable laboratory bench space. Dual detection and
specifications.
simultaneous and sequential injection deliver increased pro-
ductivity—more information from one injection. Dual analysis
provides more flexibility, more application choices, and more
information.
64 www.dionex.com
ICS-3000
Related Literature
Product Brochures

ICS-3000 System Brochure
Environmental Analysis Using the ICS-3000 Ion Chromatogra-

phy System
ICS-3000 Solutions for the Power Industry
ICS-3000 Solutions for the Semiconductor and Electronics

Industries
Product Data Sheets
ICS-3000 Reagent-Free Ion Chromatography System Data

Sheet
Contact your local Dionex representative for an ICS-3000
system customized to your application needs. See Dionex Loca-
tions in the appendices for contact information.
www.dionex.com 65
IC & RFIC Systems
ICS-2000
State-of-the-art RFIC-EG system in an integrated,
easy-to-use package
The ICS-2000 is an integrated, single-channel RFIC-EG system
that performs isocratic and gradient IC separations. With the
built-in suppressor control for the SRS and Atlas suppressors,
the system includes AutoSuppression for high performance with

unparalleled ease of use. Eluent Generation provides isocratic
and gradient electrolytic eluent concentrations, adding simplic-
ity and day-to-day consistency. The standard ICS-2000 system
includes:
• Integrated electrolytic eluent generation

• Dual-piston, isocratic, serial pump that supports 2, 3, and ICS-2000 Ion Chromatography system
4 mm i.d. columns
12
IonPac® AG15 (4 × 50 mm),
• Reliable electric PEEK Rheodyne injection valve 5
Column:
IonPac AS15 (4 × 250 mm)
Eluent: 8 mM KOH from 0–6 min,
3 6 7 8 8–55 mM from 6–18 min
• Column heater 2
Eluent Source: ICS-2000 with CR-ATC
µS 1 Flow Rate: 1.6 mL/min
Inj. Volume: 25 µL
• Electrolytic suppression 4 Temperature: 30 ˚C
3 Detection: ASRS® ULTRA (4 mm)
2 AutoSuppression® recycle mode
• Advanced digital conductivity detector with thermal control -2.0
1 220 mA
capabilities 0 10
Minutes
20 30
Peaks: 1. Fluoride 2 mg/L
2. Chloride 5
3. Nitrite 10
• Inert, non-metallic PEEK flowpath Component System 1
Retention Time
System 2
Retention Time
System 3
Retention Time 4. Carbonate -
(%RSD) (%RSD) (%RSD) 5. Sulfate 10
Fluoride 5.367 (0.062) 5.361 (0.031) 5.384 (0.017) 6. Bromide 20
• Touch screen LCD display Chloride 12.166 (0.026) 12.163 (0.022) 12.165 (0.020) 7. Nitrate 20
Nitrite 13.908 (0.022) 13.906 (0.019) 13.904 (0.021) 8. Phosphate 30
Sulfate 17.685 (0.027) 17.623 (0.025) 17.618 (0.021)
RFIC-EG systems produce consistent eluent concentrations day- Bromide
Nitrate
19.382 (0.031)
20.759 (0.036)
19.404 (0.018)
20.783 (0.014)
19.385 (0.018)
20.760 (0.017)
to-day and lab-to-lab for highly reproducible retention times and Phosphate 22.219 (0.049) 22.089 (0.043) 22.066 (0.018)
19133
(%RSD) n=20
peak areas. The ICS-2000 comes standard with integrated eluent
generation, and can be equipped with optional eluent regenera- RFIC-EG produces consistent concentrations for highly reproducible
tion. retention times and peak areas, day-to-day, system-to-system, and lab-
to-lab.
All components are easily accessed through the front chromatography

panel.
66 www.dionex.com
ICS-2000
Related Literature
Product Brochures

ICS-2000/1500/1000 Brochure
Product Data Sheets
ICS-2000 Ion Chromatography System Data Sheet
Hardware
ICS-2000 Ion Chromatography System without Degas, Full EG Control,
Chromeleon CHM-2, Windows XP Workstation.......................................... 061087
ICS-2000 Ion Chromatography System with Degas, Full EG Control,
ICS-2000 Ion Chromatography System without Degas, Full EG Control,
Chromeleon CHM-2 (no PC).......................................................................... 061091
ICS-2000 Ion Chromatography System with Degas, Full EG Control,
Chromeleon CHM-2 (no PC).......................................................................... 061090
ICS-2000 Ion Chromatography System without Degas............................... 058004
ICS-2000 Ion Chromatography System with Degas..................................... 058006
ICS-2000 Ion Chromatography System without Degas, Iso EG Control,
ICS-2000 Ion Chromatography System with Degas, Iso EG Control,
ICS-2000 Ion Chromatography System without Degas, Iso EG Control,
Chromeleon without PC................................................................................ 061099
ICS-2000 Ion Chromatography System with Degas, Iso EG Control,
Chromeleon without PC................................................................................ 061098
Accessories
ICS-2000 Ion Chromatography System Isocratic to Gradient EG upgrade.. 061378
Requires Chromeleon 6.6 or higher. Installation requires a qualified service
engineer. Eluent cartridges, EPM and mixer sold separately.
ICS-2000 Ion Chromatography System Dual EG upgrade............................ 064333
Allows eluent generation for two cartridges, as for carbonate/bicarbonate.
Requires Chromeleon 6.6 or higher.
Spare Parts Kit ICS-1000/1500/2000........................................................... 061304
Heat Exchanger Assembly (0.01" i.d.)........................................................... 059979
Heat Exchanger Assembly (0.005" i.d.)......................................................... 060943
ICS-900/1000/1500/2000 Optional Air Regulator....................................... 060054
Preventative Maintenance Kit...................................................................... 057954
Microbore Tubing Kit..................................................................................... 052324
See the RFIC-ER and RFIC-EG sections under Supplies and
Accessories for kits and accessories.
www.dionex.com 67
IC & RFIC Systems
ICS-1500 Eluent Regeneration
IC system with optional Eluent Regeneration for Add an Eluent Regeneration kit to create an RFIC-ER system,
always-on, always-ready convenience capable of continuous operation for routine isocratic applica-
tions for up to 4 weeks without the need to create new eluent.
The ICS-1500 has all PEEK components throughout, providing When left on, the system remains equilibrated and calibrated.
a contamination-free polymeric flow path. The ICS-1500 has a Just load samples and collect results!
column heater to provide day-to-day thermal stability for high-
performance applications, and a Pump Vacuum Degas capabil-

ity for outstanding eluent consistency. The standard ICS-1500
system includes: Related Literature
• Dual-piston, isocratic, serial pump that supports 2, 3, and For detailed specifications and applications, see the following
4 mm i.d. columns PDF documents under Literature on www.dionex.com.
• Reliable electric PEEK Rheodyne injection valve

Product Brochures
• Column heater
• Electrolytic suppression
Product Data Sheets
• Advanced digital conductivity detector with thermal control
capabilities ICS-1500 Ion Chromatography System Data Sheet
• Touch screen LCD display

Vacuum degas and column heater options further improve
baseline stability and yield lower detection limits. Compact and
easy to operate, the ICS-1500 delivers solid performance at an Hardware
attractive price. ICS-1500 IC System without Degas, Chromeleon and XP Workstation...... 061081
ICS-1500 IC System with Degas, Chromeleon and XP Workstation........... 061080
Note: See the RFIC-ER section under Chromatography ICS-1500 Ion Chromatography System without Degas, Chromeleon
Accessories for ER kits and accessories. CHM-1 (no PC)............................................................................................... 061085
ICS-1500 Ion Chromatography System with Degas, Chromeleon
CHM-1 (no PC)............................................................................................... 061084
Accessories
RFIC-ER Anion Startup Kit............................................................................. 067797
RFIC-ER Cation Startup Kit............................................................................ 067798
ICS-1500 Ion Chromatography System
68 www.dionex.com
ICS-1000
ICS-1000 Eluent Regeneration
Reliable operation and optional RFIC-ER, with a Add an Eluent Regeneration kit to create an RFIC-ER system,
small footprint on your bench and in your budget capable of continuous operation for routine isocratic applica-
tions for up to 4 weeks without the need to create new eluent.
The ICS-1000 has all PEEK components throughout the sys- When left on, the system remains equilibrated and calibrated.
tems providing a contamination-free polymeric flow path. The Just load samples and collect results!
ICS-1000 has an optional column heater to provide day-to-day

thermal stability for high-performance applications and a Pump
Vacuum Degas capability for excellent eluent consistency.
Automate the system with an AS40 or AS autosampler for fully- Related Literature
automated control. The standard ICS-1000 system includes:
• Dual-piston, isocratic, serial pump that supports 2, 3, and PDF documents under Literature on www.dionex.com.
4 mm i.d. columns
Product Brochures
• Reliable electric PEEK Rheodyne injection valve
• Optional column heater
• Electrolytic suppression Product Data Sheets
• Advanced digital conductivity detector with thermal control ICS-1000 Ion Chromatography System Data Sheet
capabilities
• Informative front LED panel for system status monitoring Ordering Information
Vacuum degas and column heater options further improve
baseline stability and yield lower detection limits. Compact and Hardware
easy to operate, the ICS-1000 delivers solid performance at an ICS-1000 Ion Chromatography System Without Degas, CM SE,
attractive price. The ICS-1000 is available with Chromeleon SE, Windows XP Workstation............................................................................. 061076
which allows control of a single ICS-1000 with autosampler. ICS-1000 Ion Chromatography System With Degas, CM SE, Windows
XP Workstation.............................................................................................. 061077
Note: See the RFIC-ER section under Chromatography ICS-1000 Ion Chromatography System without Degas, Chromeleon
Accessories for ER kits and accessories. SE, without PC............................................................................................... 061079
ICS-1000 Ion Chromatography System with Degas, Chromeleon SE,
without PC..................................................................................................... 061078
Accessories
Column Heater............................................................................................. 058060T
RFIC-ER Cation Startup Kit............................................................................ 067798
ICS-1000 Ion Chromatography System Microbore Tubing Kit..................................................................................... 052324
www.dionex.com 69
IC & RFIC Systems
ICS-900
ICS-900 integrated starter line ion chromatography
system
The ICS-900 is an integrated single-channel ion chromatogra-
phy system designed to run specific isocratic anion and cation
applications. The system uses MMS 300 suppression with Dis-
placement Chemical Reagent (DCR) technology for low noise

and stable baselines. Each ICS-900 system has an all polymeric
flow path with a reliable dual piston pump, high pressure pulse
damper, electrically-actuated PEEK Rheodyne valve, and a
temperature controlled conductivity cell.
• Starter line IC designed specifically for routine analysis

• MMS suppression with DCR technology for low noise and ICS-900 Ion Chromatography System
fast start-up
• Contamination-free with inert material throughout the
fluidic flow path
• High-performance digital, thermally controlled conductiv-
ity detection
• Supports 2 mm, 3 mm, and 4 mm i.d. columns
The ICS-900 can be ordered with Chromeleon SE, which allows

control of a single ICS-900 with autosampler for an attractive
price.
Note: See the Chromatography Accessories section for

ICS-900 consumables kits.
Easily access ICS-900 system components.
70 www.dionex.com
ICS-900
Conductivity
Cell
Regenerant Inn ICS-900 Systems
Waste
Wa
W asste Eluent In Suppressorr
to Suppresso
to Regenerant
Reg
egenerant
n Bottle
Bottttle
le Suppressed Eluent In ICS-900 Ion Chromatography System with Chromeleon 6.8 SE and
to Detector Cell
Windows XP Workstation............................................................................. 067552
ICS-900 Ion Chromatography System with Chromeleon 6.8 SE.................. 067551

ICS-900 Ion Chromatography System Expansion Unit, requires
MMS 300
existing ICS-900 and software...................................................................... 067550
Adds a second system to an existing ICS-900
ICS-900 Installation Service. ....................................................................... 060124
Regenerant Out
to Waste Includes system set-up and testing. Extends warranty coverage on hardware
and software to one year.
Eluent In
System Waste
Schematic of the ICS-900 DCR flow path.

ICS-900 and ICS-90 Accessories
Preventive Maintenance Kit.......................................................................... 057954
Related Literature
For more details, see the following PDF document under Litera-
ture on www.dionex.com.
Product Brochures
ICS-900 Brochure
Product Datasheets
ICS-900 Ion Chromatography System Data Sheet
www.dionex.com 71
IC & RFIC Systems
72 www.dionex.com
IC & RFIC Modules
From basic autosampling to ultrasensitive detection,
Dionex IC module offerings are unmatched
The IC product family includes an extensive set of fully inert,
PEEK-based modules engineered for high performance, ease-

of-use, and reliability. We offer a wide variety of autosamplers,
injectors, pumps, thermostatted column compartments, and
detectors with reliable, precise, and accurate operation. Whether
you require a dedicated system or modularity for flexible
applications, all components are integrated and single-point Eluent Generation: Eluent Generation is a part of RFIC and
controlled through Chromeleon software or easy-to-use TTL empowers laboratories to automatically generate high purity
control. eluents.
• Automated eluent generation for simplicity and reliability IC & RFIC Pumps: Completely inert, robust pump design
capable of incredible acuracy and precision
• Single and dual isocratic and gradient pumps
IC & RFIC Injectors & Autosamplers: State-of-the-art automa-
• Autosamplers from basic to state-of-the-art automation and tion and sample care with the all-inert AS, high-volume AS-HV,
high-volume sample prep and economical AS40 autosamplers.
• Column and detector compartments that integrate thermal IC & RFIC Column Compartments: IC & RFIC column
control with sample prep and injection compartments and column thermostats for high retention time
stability.
• High performance detectors, including conductivity, elec-
trochemical, optical, and MS IC & RFIC Detectors: Conductivity; electrochemical; and
single wavelength, multiple wavelength, and photodiode array
Note: See the Mass Spectrometry section for MS detectors. optical absorbance detectors.
Postcolumn Reaction Systems: Postcolumn derivatization can be
used to extend the detection limits of ions that would otherwise
exhibit limited sensitivity.
www.dionex.com 73
IC & RFIC Modules
Eluent Generation ICS-3000 EG Eluent Generator
The ICS-3000 EG Eluent Generator simplifies ion chromatogra-
phy. Capable of dual eluent generation, the EG produces eluent
automatically. With improved electronics, noise filtering, and
precision control, smoother eluent delivery with minimal noise
will give you greatly improved peak detection.
• Generates high-purity, carbonate-free hydroxide eluent

from deionized water
• Minimizes baseline drift
• Improves retention time stability and resolution
• Provides excellent run-to-run reproducibility
• Compatible with both gradient and isocratic applications
• Delivers eluent concentrations from 0.01 up to 100 mM
Eluent Generation is an essential part of RFIC systems, empow-
• Extends the lifetime of pistons and pump seals by pumping
ering users to generate high purity eluents with a mouse click
only water
and to perform electrolytically generated gradient analysis with
our isocratic pump. • Built-in leak sensor and operator alert for maximum system
safety
Note: See also RFIC-Eluent Regeneration under
Chromatography Accessories for EluGen eluent generation To make Reagent-Free IC (RFIC ) systems compatible with
cartridges and CR-TC trap columns. even more applications, we have expanded eluent generation
with carbonate/bicarbonate cartridges. In addition to hydrox-
ide and methanesulfonic acid eluents, now you can generate
carbonate-based eluents as well. Just add water to generate the
Related Literature
perfect eluent for your application automatically.
PDF documents under Literature on www.dionex.com. Key Specifications
Minimum and Maximum Eluent Concentrations: 0.01 up to
Product Brochures
100 mM
Reagent-Free Ion Chromatography Eluent Flow Rates: 0.10–3.00 mL/min
ICS-3000 System Brochure Eluent Types: KOH, LiOH, NaOH, K2CO3/KHCO3, MSA
Product Data Sheets Maximum Operating Pressure: 21 MPa, 3000 psi
Reagent-Free Ion Chromatography Systems with Eluent Gen- Maximum Solvent Concentration: no solvent when using EGC-
eration for IC Without Manually Prepared Eluents MSA; 25% methanol with EGC-KOH, NaOH, LiOH
Reagent-Free Controller for Ion Chromatography Operating Temperature Range: 4–40 °C
ICS-3000 Reagent-Free Ion Chromatography System Data Operating Humidity Range: 5–95% relative, noncondensing
Sheet Dimensions (h × w × d): 41 × 23 × 56 cm (16 × 8.75 × 21.5 in.)
Weight: 25 kg (40 lb)
Power Requirements: 90–265 V ac, 47–63 Hz
Hardware
Eluent Generator Module.............................................................................. 061714
EG Second Cartridge Add-On Kit.................................................................. 062137
74 www.dionex.com
Eluent Generation
RFC-30 Reagent-Free Controller RFC-10 Reagent-Free Controller
The RFC-10 Reagent-Free Controller can be ordered to provide
continuously variable current (with 1 mA resolution) to the
Atlas and SRS suppressors when using DX-600 or DX-500
systems configured with an ED40/50 or CD20/25 detector; the
DX-320 with an IC25 detector; or the DX-120. The DX-120
requires an adapter cable (P/N 057861) for the RFC-10.

Hardware
RFC-10 Reagent-Free Controller................................................................... 060335
Accessories
The RFC-30 Reagent-Free Controller enables you to experience DX-120 Adapter Cable.................................................................................. 057861
the ease of use, cost savings and simplicity that Reagent-Free IC An adapter cable is required to interface the RFC-10 or RFC-30 to the DX-120.
offers. Reagent-Free IC is the powerful combination of Dionex
“Just Add Water” eluent-generation technology, plus electrolytic
suppression and the continuously regenerated trap column (CR-
TC). Standard RFC-30 System includes:
• RFC-30 Reagent-Free Controller with EGC-KOH Car-

tridge AND
• CR-ATC Continuously Regenerated Anion Trap OR
• RFC-30 with EGC-MSA Cartridge AND
• CR-CTC Continuously Regenerated Cation Trap
Reagent-Free IC eliminates variability and potential contamina-

tion, providing a stable baseline, higher sensitivity, resolution,
and maximum reproducibility. Reagent-Free IC greatly reduces
the complexity of an IC system, allowing you to focus on what
matters—your application and results. Reagent-Free IC is avail-
able exclusively from Dionex.
Note: For use with DX-120, DX-320, DX-500, DX-600,

or ICS-2500 ion chromatography systems. DX-120 bundle
includes a column temperature stabilizer.
Hardware
RFC-30 Reagent-Free Controller (EGC-KOH, CR-ATC).................................. 060667
RFC-30 Reagent-Free Controller (EGC-MSA, CR-CTC)................................. 060668
RFC-30 (KOH, CR-ATC, CTS-10) for DX-120 Only......................................... 061413
Use with EGC II KOH cartridge, CR-ATC.
RFC-30 (MSA, CR-CTC, CTS-10) for DX-120 Only........................................ 061414
Use with EGC II MSA cartridge, CR-CTC.
Accessories
DX-120 Adapter Cable.................................................................................. 057861
www.dionex.com 75
IC & RFIC Modules
IC & RFIC Pumps Flow Rate Precision: <0.1%
Gradient Proportioning Accuracy and Precision: ±0.5% at
2 mL/min
Pressure Range: 0.35–35 MPa (50–5000 psi)
Dimensions (h × w × d): 36 × 21 × 48 cm (14 × 8.25 × 19 in.)
Weight: DP: 24.1 kg (53 lb.)

Additional Specifications: refer to ICS-3000 Reagent-Free Ion
Chromatography System Data Sheet
Hardware
The ICS-3000 pumps are dual-piston, serial design pumps that DP Gradient - Gradient Dual Pump with Degas........................................... 061712
support micro- and standard bore formats. The ICS-3000 pump DP Gradient - Isocratic Dual Pump with Degas............................................ 061710
comes in either a Single (SP) or Dual (DP) format in the same DP Isocratic - Isocratic Dual Pump with Degas............................................ 061708
bench top footprint. The pump is upgradeable from a single to
a dual pump to meet your needs now and in the future. Low-
pressure quaternary gradients are available for linear and curved ICS-3000 SP Single Pumps
gradients.
The ICS-3000 SP Single Pumps provide flow rate ranges of
0.001 to 10 mL/min, to support microbore, standard, and even
semipreparative applications. The pump is upgradeable from a
ICS-3000 DP Dual Pumps
single to a dual pump, so you can configure your system to meet
The ICS-3000 DP Dual Pump configurations provide indepen- your needs. Gradient pumps offer linear, concave, and convex
dent pumping capabilities in the same module and space saving gradients and come with a low volume passive mixer and seal
footprint. Flow rate ranges of 0.001-10 mL/min support micro- wash.
bore, standard, and even semipreparative applications. Gradient
• Inert, PEEK pump heads, mixing chamber, and flow paths
pumps offer linear, concave, and convex gradients and come
with a low volume passive mixer and seal wash. • Integrated piston seal wash
• Inert, PEEK pump heads, mixing chamber, and flow paths • Easy access to all fluidics
• Integrated piston seal wash • Built-in vacuum degas
• Easy access to all fluidics • User selectable upper and lower pressure limits for pump
shut down
• Built-in vacuum degas
• Field upgradeable to DP Dual Pump configuration
• User selectable upper and lower pressure limits for pump
shut down
Key Specifications
Benefits of Dual Pump Configurations: Dual analysis, such as Flow Rate Range: 0.001 to 10.000 mL/min
anions and cations from a single injection. Sample preconcen-
tration or matrix elimination. Post column reagent delivery. Flow Rate Accuracy: <0.1%
External water or chemical regenerant delivery. A backup pump
Flow Rate Precision: <0.1%
for your primary application. Consumables cleanup or startup
preparation, preventing primary system downtime. Gradient Proportioning Accuracy and Precision: ±0.5% at
2 mL/min
Key Specifications Pressure Range: 0.35–35 MPa (50–5000 psi)
Flow Rate Range: 0.001 to 10.000 mL/min Dimensions (h × w × d): 36 × 21 × 48 cm (14 × 8.25 × 19 in.)
Flow Rate Accuracy: <0.1%
76 www.dionex.com
IC & RFIC Pumps
Weight: SP: 20.4 kg (45 lb.) Ordering Information
Hardware
Additional Specifications: refer to ICS-3000 Reagent-Free Ion
Chromatography System Data Sheet EO Eluent Organizer with four 2 L Bottles.................................................... 062628
EO Eluent Organizer (tray only)..................................................................... 062590

Accessories
Hardware EO Regulator Accessory and Stand (For mounting on the DC).................... 063493
EO Regulator Accessory and Stand (For mounting on the TC/VWD/PDA).. 064792
SP Gradient Pump with Degas...................................................................... 061706
EO Regulator Accessory (to be added to 063493)........................................ 064387
SP Isocratic Pump with Degas...................................................................... 061704
Bottle, 1 L, Plastic, ea.................................................................................... 063291
SP Isocratic Pump without Degas................................................................. 061702
Gradient Pump Upgrade, SP to DP................................................................ 061700
Isocratic Pump Upgrade, SP to DP................................................................ 061701
Bottle, 1 L, Glass, ea..................................................................................... 062595
Used to convert a SP Pump to a DP Pump. This upgrade requires a Service Call
by a Dionex Representative. Bottle, 2 L, Glass, ea..................................................................................... 061729
EO Eluent Organizer and Accessories AXP Auxiliary Pump
The EO Eluent Organizer, constructed of corrosion-proof poly- The AXP Auxiliary Pump is a single-piston, isocratic metering
propylene, is designed to hold eluent containers and organize pump ideal for ion chromatography sample and reagent deliv-
eluent tubing and air lines. ery. The chemically inert, metal-free, PEEK-based flow path
eliminates corrosion and contamination problems when using
• Holds four 1 or 2 liter plastic, two 1 liter glass or one 2 liter acidic or alkaline reagents, or common water-miscible solvent.
glass bottles
• Digital control and status
• Up to two EO organizers stack on top of a DC or TC mod-
ule • Wide flow rate range
• Translucent liner contains spills and allows view of liquid • Internal pulse damper
levels • Compatible with IC and bio applications
• Constructed of corrosion-proof polypropylene and epoxy • Standard positive seal washing
• Pressure regulator option is available • Easy maintenance
• Flexible, advanced Chromeleon control
• Convenient size
www.dionex.com 77
IC & RFIC Modules
Key Specifications
Pressure Range: 0.35–17.5 MPa (50–2500 psi)
Flow Rates: 0.01–9.99 mL/min
Flow Accuracy: 3% throughout flow range
Pressure Pulsation: 2% peak-to-peak at 100 psi, 1 mL/min with
in-line pulse damper

Flow Rate Precision: 0.5% at calibration pressure
Dimensions (h × w × d): 16.5 × 16.5 × 25.4 cm
(6.5 × 6.5 × 10 in)
Weight: 6.8 kg (15 lbs)
Power Requirements: 120–230 V ac; 50–60 Hz
Materials: PEEK, ceramic, and inert polymers
Control: Chromeleon (USB), front panel, and relay control
Hardware
AXP Auxiliary Pump....................................................................................... 063973
78 www.dionex.com
IC & RFIC Injectors & Autosamplers
IC & RFIC Injectors & • All-PEEK flow paths, compatible with aqueous and
reversed-phase eluents, safe from metal contamination
Autosamplers
• Moving-needle design to guarantee reliable sampling from
a variety of vial sizes
• Easy syringe priming
• Sample Prep option to automate sample and standard

preparations, saving time and labor
• Well Plate capabilities, optional sample tray cooling
The AS features can be controlled through either Chromeleon

Chromatography Management Software or the module front
panel.
Key Specifications
Sample Capacity (Vials): 49 ea. 10 mL; 100 ea. 1.5 mL
Dionex injectors and autosamplers have completely metal-free Sample Capacity (Well Plates): 2 ea. 2 mL 96 deep well plate or
flow paths, including the injection loop and autosampler needle. 2 ea. 0.5 mL 96 well plate
A selection of autosamplers is available to fit your automation
Injections per Vial: 1–99
needs.
Minimum Sample Volume: 10 µL can be sampled from a 300 µL
microvial; 20 µL can be sampled from a 500 µL microvial
Related Literature Variable Volume Range: 1–100 µL in 0.1 µL increments;
100–8200 µL in 1 µL increments
PDF documents under Literature on www.dionex.com. Injection Precision: fixed loop <0.3% RSD at 20 µL; Partial
loop <0.5% RSD at 20 µL
Product Brochures Dilution Precision: <1.0% RSD for a 1:100 dilution
IC AS, AS40, and AS-HV Autosamplers Dispensing Precision: <0.2% RSD (by weighing)
Product Data Sheets Carryover: <0.01% with 500 µL flush volume
AS40 Autosampler Datasheet Operating Temperature Range: 4–40 ºC (40–104 ºF)
Simultaneous Injection AS Autosampler Power Requirements: 90–265 V ac, 47–63 Hz
ICS-3000 Reagent-Free Ion Chromatography System Data Ordering Information

Sheet
Hardware
AS Autosampler
AS Autosampler, Base Configuration........................................................... 063102
The Dionex AS Autosampler is designed for high precision, AS Autosampler, with Sample Preparation Option...................................... 063103
reliability, ruggedness, and ease of use. The AS can be used AS Autosampler, Simultaneous Injection Configuration without Valves.... 063104
with the entire ICS product family. The AS Autosampler is a AS Autosampler, Simultaneous Injection with Sample Prep Option.......... 063105
low-cost, metal-free, rugged, automated sample loading device
AS Autosampler, with Sample Tray Temperature Control Option............... 063106
designed especially for ion chromatography applications.
AS Autosampler, with Sample Prep and Tray Temp Control Options.......... 063107
• Capable of simultaneous injection without cross contami- AS Autosampler, Simultaneous Injection Configuration with Internal
nation of chemistries Valves............................................................................................................. 063101
AS Autosampler, with Injection Valve.......................................................... 061786
• Consistent operation at RSDs less than 0.3% for reproduc-
AS Autosampler, with Injection Valve and Sample Tray Temperature
ible and accurate results
Control............................................................................................................ 061788
• 10 mL polystyrene sample vials with wide openings for
large-volume injections and trace analysis
www.dionex.com 79
IC & RFIC Modules
Kits AS40 Autosampler
Sample Prep Tower Kit.................................................................................. 063916
Inject Port Line Extension Kit for Second AS............................................... 062578
AS Diverter Valve for Sequential Injection................................................... 063294
Upgrade Kit to Simultaneous for AS/AS50 (USB)........................................ 063742
Valve Rebuild Kit........................................................................................... 057896
Rheodyne 10-Port Valve Rebuild Kit............................................................. 061759

Preventive Maintenance Kit for AS50 (USB) and AS................................... 060581
AS Autosampler Spare Parts Kit................................................................... 064762
Well Plate and Vial Trays

AS 10 mL Vial Plastic Tray, 49 Vial Capacity................................................ 062374
AS 2 mL Vial Cast Tray, 99 Vial Capacity...................................................... 062481
AS 2.0 mL Vial Insulated Cast Tray with Covers, 99 Vial Capacity.............. 063442
Micro-Well Plate Tray, Includes Two Well Plates with Round (U)
Shaped 0.5 mL Wells & Covers.................................................................... 066324 The AS40 Autosampler is an economical metal-free, sample
Micro-Well Plate Tray. Includes Two Well Plates with Conical (V) loading device designed to work with existing injection valve(s)
Shaped 0.45 mL Wells and Covers............................................................... 066331 in the system. The AS40 holds up to 11 cassettes of either six
Deep-Well Plate Tray. Includes Two, 2 mL Well Plates and Covers........... 066325 5 mL vials or eight 0.5 mL vials. Up to three injections can be
made from each vial. Sample can be filtered during loading
Vial Kits through a 20 µm filter in the vial cap.
Vial Kit,10 mL Polystyrene w/Caps & Septa,100 each................................ 055058 • Simple front-panel layout makes the AS40 easy to learn
Vial Kit 1.5 mL Glass w/Caps & Septa, 100 each........................................ 055427 and use.
Vial Kit, 0.3 mL Polypropylene w/Caps & Septa, 100 each......................... 055428 • Metal-free flow path eliminates corrosion and metal con-
Vial Kit, 1.5 mL Polypropylene with Caps & Septa, Pkg of 100................... 061696 tamination problems.
• Accommodates disposable PolyVial sample vials in both
Well Plates and Well Plates Covers
5 mL and 0.5 mL sizes.
Well Plates, 96 Micro-well, 0.5 mL Round (U) Shaped Wells
(Pkg of 10). Not suitable for IC..................................................................... 066332 • Filter caps automatically filter the samples during delivery.
Well Plates, 96 Micro-well, 0.45 mL Conical (V) Shaped Wells • Sample can be loaded to loops or concentrator columns.
(Pkg of 20). Not suitable for IC..................................................................... 066333
Well Plates, 96 Deep-Well, 2 mL Wells (Pkg of 5). Suitable for IC • Bypass valve displaces excess sample or trapped air before
Where Concentration of Ions is Above 1 ppm............................................. 066334 sampling.
Well Plate Covers (Pkg of 10) for 0.45 mL and 0.5 mL Well Plates............. 066335 • Rinse vials can be placed anywhere in the sequence.
Well Plate Covers (Pkg of 10) for 2 mL Well Plates..................................... 066330
• The output relay function indicates if and when an au-
tosampler load was completed.
Syringes
Sample Syringe, 100 µL................................................................................ 055064 Control is provided from a relay output. Samples can be loaded
Sample Syringe, 250 µL................................................................................ 053916 against backpressures up to 690 kPa (100 psi) without external
Sample Syringe, 500 µL................................................................................ 055065
sampling pump, facilitating preconcentration work. Rinse vials
can be placed anywhere in the sequence to flush lines or recon-
Sample Syringe, 1.0 mL................................................................................ 055066
dition concentrator columns. Two boxes of cassettes and one
Sample Prep Syringe, 2.5 mL........................................................................ 055067 box of PolyVials with filter caps are included with the sampler.
Sample Prep Syringe, 5.0 mL........................................................................ 053915
Sample Prep Syringe, 10.0 mL...................................................................... 055068 Note: The AS40 automatic switching power supply for
universal input voltage (90–265 V ac) simplifies instrument
installation and setup.
80 www.dionex.com
IC & RFIC Injectors & Autosamplers
Key Specifications Accessories (Continued)
Sample Delivery Method: positive displacement against Filter Caps, 0.5 mL, Pkg of 250...................................................................... 038011
backpressures of up to 690 kPa (100 psi) Plain Caps without Filters, 5.0 mL, Pkg of 250............................................. 039528
Plain Caps, 0.5 mL, Pkg of 250...................................................................... 042154
Capacity: 11 cassettes of either six 5 mL or eight 0.5 mL
PolyVials AS40 Simultaneous Injection Kit for ICS Systems....................................... 060258
Allows you to direct sample to two independent ion chromatography systems
Vial Size: 0.5 mL or 5 mL with just one AS40

Filter Pore Size: 20 µm
Minimum Volume Delivered: 0.5 mL vials: 0.17 mL per AS-HV Autosampler
injection; 5.0 mL vials: 1.7 mL per injection
External Control: Input: Load; Output: Ready
Injections per Vial: 1, 2, or 3
Loop Injection: delivers sample rapidly to sample loop
(~4 mL/min)
Concentrator Injection: delivers sample against backpressure of
up to 690 kPa (100 psi) at ~0.4 mL/min (0.5 mL vials) or
1 mL/min (5 mL vials)
Proportional Injection Mode: delivers volume inversely propor-
tional to the number of injections per vial
Constant Injection Mode: delivers fixed volume independent of
the number of injections per vial
Bleed Function Feature: On: displaces excess sample and any The AS-HV is a large volume autosampler that can be pro-
trapped air via bypass valve before sampling; Off: delivers grammed to execute a series liquid handling steps. It has a
sample and any air directly to inj. valve sample capacity of up to 250 mL and through the use of either
Power Supply: automatic switching power supply for universal a syringe pump, peristaltic pump, or piston pump, can deliver
input voltage, 90–265 V ac, 47–63 Hz sample to an injection valve loop or a concentrator column.
Dimensions (h × w × d): 28.7 × 36.3 × 44.5 cm • Non-metallic (all-PEEK) flow path

(11.3 × 14.3 × 17.5 in.)
• Volumes up to 250 mL with extremely pure tissue culture
Weight: 8.9 kg (195 lb) flasks or poly bottles
• Rapid, precise arm motion speeds up sampling time to
Ordering Information maximize efficiency
• Supports "push" or "pull" sample loading
Hardware
• Multiple loading pumps to suit your budget and your ap-
AS40 Automated Sampler with Starter Vial Kit (5 mL)................................ 056830
plication
AS40 Automated Sampler with Starter Vial Kit (0.5 mL)............................. 056829
• Fully Supported under Chromelon 6.8 or greater
Accessories
Note: See also the AXP Auxiliary Pump and Peristaltic Pump
AS40 Cassettes, 6 Position, 5 mL, Box of 6................................................. 046032 under IC & RFIC Pumps.
AS40 Cassettes, 8 Position, 0.5 mL, Box of 6.............................................. 046031
Poly Vials and Filter Caps, 5.0 mL, 250 each................................................ 038141
Poly Vials and Filter Caps, 0.5 mL, 250 each................................................ 038142
Poly Vials and Plain Caps without Filters, 5.0 mL, 250 each....................... 039532
Poly Vials and Plain Caps, 0.5 mL, 250 each................................................ 042014
Poly Vials, 5.0 mL, Pkg of 250....................................................................... 038008
Poly Vials, 0.5 mL, Pkg of 250....................................................................... 038010
Filter Caps, 5.0 mL, Pkg of 250...................................................................... 038009
www.dionex.com 81
IC & RFIC Modules
Key Specifications MX Six-Port Injection Valve
Sampler Capacity: 24 with 250 mL tissue culture flask; 15 with
250 mL narrow mouth Nalgene bottle
Sample Protection: pre-split septum with cap and/or Plexiglas
Protective Cover for the entire AS-HV
Vial Size: 250 mL
Maximum Injection Volume: application dependent (up to

250 mL)
Minimum Sample Volume: sample loop dependent
Number of Injections per Vial: variable
Injection Type: full loop /concentrator
Injection Valve: none, AS-HV relies on a valve in the IC system
Injection Precision—Sample Loop: <0.3%
The Rheodyne MX Series II automated fluidic valve provides
Injection Precision—Concentrator: <2% added flexibility where stand-alone injection is required. Easily
combine the MX with the valves in your existing instrument to
Injection Valve Carry-Over—Sample Loop: <0.1% support complex fluid switching and sample injection needs.
Injection Valve Carry-Over—Concentrator: <0.2%
Sample Loading: push or pull Ordering Information
Software Control: Program and Sequence Wizard

Hardware
Software Compatibility: Chromeleon 6.8 or greater
Rheodyne, 6 Port, Automated Injection Valve.............................................. 063573
Hardware
AS-HV with Integrated Wash Station.......................................................... 064051
AS-HV Syringe Pump with 10 mL Syringe.................................................... 064506
AS-HV AXP Pump.......................................................................................... 064507
Peristaltic Pump............................................................................................. 064508
Accessories
250 mL Tissue Culture Flasks with Caps and Septa - Pkg of 50.................. 064235
250 mL Nalgene Poly Bottles with Caps and Septa - Pkg of 72.................. 064232
Trace Analysis Cover..................................................................................... 064052
Sample Rack for 250 mL Nalgene Bottles. 15 Position.............................. 064234
Sample Probe - PTFE Sleeved and Lined...................................................... 064056
PEEK Probe..................................................................................................... 064254
Standards Rack Kit - Six Position.................................................................. 064252
Sample Syringe 10 mL.................................................................................. 064224
USB to RS-232 Cable..................................................................................... 064261
Rheodyne, 6 Port, Automated Injection Valve.............................................. 063573
Relay Cable for Externally Controlled Pump................................................. 064350
82 www.dionex.com
IC & RFIC Column Compartments
IC & RFIC Column Compartments • Column ID chip system for monitoring column properties
and usage
• Optional heat exchangers for optimal eluent temperature
and improved reproducibility
Key Specifications

Temperature Range: 5–85 ºC (maximum cooling 18 ºC below
ambient)
Temperature Accuracy: ±0.5 ºC
Temperature Stability: ±0.1 ºC
Temperature Precision: ±0.1 ºC
Column Capacity: three columns; max. 30 cm in length
Heat-Up/Cool-Down Time: typically 15 min from 20 ºC to
Dionex offers a variety of column compartments to organize 50 ºC; typically 15 min from 50 ºC to 20 ºC
columns and achieve precise thermostatting for ultimate reten- Inputs/Outputs: two digital inputs/outputs, two relay outputs
tion time reproducibility.
Dimensions (h × w × d): 17.4 × 44.4 × 50.3 cm
(6.8 × 17.5 × 20 in.)
ICS-3000 Thermal Compartment Power Requirements: 90–265 V ac, 47–63 Hz
Operating Temperature Range: 10–35 ºC (50–95 ºF)
Operating Humidity Range: 5–80% relative, noncondensing
Hardware
Thermal Compartment, No Valves................................................................ 064659
Thermal Compartment, One 6-Port Valve, PEEK.......................................... 064660
Thermal Compartment, Two 6-Port Valves, PEEK........................................ 064661
Thermal Comp, 6-Port Valve, 10-Port Valve, PEEK...................................... 064651
Get the benefits of better separations and reproducibility

with precise temperature control. The ICS-3000 TC Thermal
Compartment for use with optical or MS detection enhances
performance by integrating column temperature regulation with
injection valves. This maximizes thermal stability and mini-
mizes extra-column volumes.
• Wide temperature range of 5–85 ºC

• Precise temperature control
• Fast heat up and cool down times
• Single-zone oven minimizes tubing lengths for less delay
volume and better peak efficiency
• Single and dual valve configurations
www.dionex.com 83
IC & RFIC Modules
Detector/Chromatography Temperature Stability: ± 0.2 °C
Compartment Temperature Precision: ± 0.2 °C
Lower Column Section: injection valves: 1 or 2 ea., 6- or
10-port, standard bore (SB) or micro bore (MB)
Column Capacity: up to two column sets, 1–9 mm; maximum
column length: 250 mm plus 50 mm guard column; precolumn
heat exchangers: 2
Automation Manager (Option): 2 high-pressure injection valves,
6- or 10-port; 2 low-pressure valves, 2- or 3-way
Reaction Coil Heater (Option): 5 °C above DC temperature to
80 °C; 2 coils
Leak Detection: optical leak sensor
Dimensions (h × w × d): 44.5 cm × 42 cm × 57 cm
This compartment houses the detector, suppressor control, (17.5 in × 16.5 in × 22.5 in)
column thermal controller, and the preparation module. This
combination provides complete tubing thermal stability and Ordering Information
short connections between components. The CD and ED detec-
tors are easy to install for immediate use.
DC Compartment
• Innovative electrochemical detection technology DC with Single Temperature Zone, One Injection Valve, SB....................... 063770
• CD conductivity detector features improved electronics for DC with Single Temperature Zone, Two Injection Valves, SB..................... 063771
superior detection DC with Dual Temperature Zones, One Injection Valve, SB........................ 063772
DC with Dual Temperature Zones, Two Injection Valves, SB...................... 063773
• Dual conductivity and electrochemical detection provides
DC with Single Temperature Zone, One Injection Valve, MB...................... 061790
increased flexibility and utility
DC with Single Temperature Zone, Two Injection Valves, MB.................... 061791
• Optional AM Automation Manager with extra valves for DC with Dual Temperature Zones, One Injection Valve, MB....................... 061792
eluent addition and sample handling
DC with Dual Temperature Zones, Two Injection Valves, MB..................... 061793
• Supports AutoPrep, preconcentration, AutoNeutralization,
matrix elimination, and postcolumn reagent addition Automation Manager
• Valve control integral to software, eliminating complicated AM Automation Manager with One 6-Port High-Pressure Valve and
TTL or relay programming One Low-Pressure 3-Way Valve................................................................... 061740
AM Automation Manager with One 10-Port High-Pressure Valve and
With the DC's flexible configuration and Chromeleon control, One Low-Pressure 3-Way Valve................................................................... 061736
your possibilities have more than doubled. Configure your AM Automation Manager with Two 10-Port High-Pressure Valves
system for simultaneous injection and perform both anion and and Two Low-Pressure 3-Way Valves.......................................................... 061738
cation analysis with one injection. Perform two different anion Reaction Coil Heater RCH-1.......................................................................... 061746
applications. Or eliminate dilutions from unknown samples by
Knitted Reaction Coil, 500 µL, Potted (PCH-2).............................................. 039349
running the same application with large loop/small loop injec-
Spooled Reaction Coil, 750 µL (PCH-2)......................................................... 037859
tions.
Key Specifications
DC Models: available in both a Single Temperature Zone Model
and a Dual Temperature Model
Temperature Range: single-temperature model: 15–40 °C; dual-
temperature model: upper section 15–40 °C,
lower section 10–70 °C
Temperature Accuracy: ± 0.5 °C
84 www.dionex.com
IC & RFIC Column Compartments
ICS-1000/1500/2000 Column Heater
Temperature control promotes column stability and reproduc-
ibility in many applications. A low-dispersion heat exchanger
provides pre-heating prior to the column and ensures the column
temperature set point. The transparent cover allows viewing of
the column connections without temperature disruption.

Note: The column heater is standard on the ICS-1500 and
ICS-2000 and optional on the ICS-1000.
Hardware
Column Heater............................................................................................. 058060T
www.dionex.com 85
IC & RFIC Modules
IC & RFIC Detectors ICS-3000 CD Conductivity Detector
Dionex detectors are designed for high sensitivity and minimum The CD Conductivity Detector handles any IC application, from
peak dispersion in all PEEK flow path making the detectors single column methods with high background signals to deter-
compatible with eluents of pH 0–14. mination of trace contaminants in high purity water, without
cumbersome range change distortions.
• ICS-3000 CD conductivity detector with integrated design
and plug-and-play capability • Microprocessor-controlled digital signal processing detects
high and low concentrations
• The ICS-3000 ED, the latest, most innovative electrochemi-
cal detector known • Large dynamic range-up to 15,000 µS/cm
• VWD Variable Wavelength Detector in either single or four • Snap-in design, no cables or tools required for installation
wavelength configuration
• Minimizes noise while maximizing sensitivity
• 1024-element PDA Photodiode Array Detector for broad
• Electronics integrated between the cell and detector for
spectral range high-resolution 3-D absorbance detection
greater stability
See also the Mass Spectrometry section. • Independent temperature control, separate from the rest of
the DC module
Key Specifications
Electronics Type: microprocessor-controlled digital signal
processing
Detection Type: single and dual conductivity detection
Cell Drive: 8 kHz square wave
Linearity: 1% at 1000 µS/cm
Resolution: 0.00238 nS
Output Range: 0–15,000 µS, digital or analog
Electronic Noise, Wet: <0.2 nS at 23 µS/cm background,
<0.1 nS at 1 µS/cm background
Filter: rise times of 0–10 s
Temperature Compensation: default at 1.7%; programmable at
0–3%
Autoranging: autoranging digital conductivity signal monitoring
with Chromeleon software
86 www.dionex.com
IC & RFIC Detectors
Ordering Information Potential Range: -2 V to + 2 V in 0.001 V increments
Signal Range, Digital and Analog: DC amperometry: 5 pA to
Hardware 74 µA; integrated amperometry: 50 pC to 200 µC
CD Conductivity Detector and Integrated Cell............................................. 061716 Filter: 0–10 s response time
Remote Detector Housing for CD................................................................. 063725
Control Mode: local or remote using relay closures, TTL, of
Chromeleon using DC module

ICS-3000 ED Electrochemical Detector
Cell Body: titanium body and inlet tubing, compatible with
2 and 4 mm id columns; pressure rating 120 psi when fully
assembled
Working Electrodes: disposable gold, silver, and platinum; con-
ventional gold, silver, platinum, and glassy carbon
Reference Electrode: pH-Ag/AgCl combination
Autoranging: autoranging digital amperometry with
Chromeleon
Analog Output: user-selectable full scale of 10, 100, or
1000 mV
Working Cell Volume: <0.5 µL
Maximum Operating Pressure: 0.7 MPa (100 psi)
The ICS-3000 ED Electrochemical Detector features a new cell Ordering Information

design along with a newly designed reference electrode. The
cell is more rugged due to the new yoke-knob assembly, which Hardware
provides consistent compression to the working electrode and
cell gasket. Exciting innovations will create new opportunities ED Electrochemical Detector (without cell).................................................. 061718
for your laboratory.
Accessories
• One-piece combination pH and Ag/AgCl reference elec-
ED Cell with Reference Electrode and Spacer Block (no working
trode for consistency and reliability electrode)....................................................................................................... 061756
• Yoke-Knob design for reproducible installation of working ED Cell pH Reference Electrode................................................................... 061879
electrode and cell gasket Disposable Gold Electrodes for Amino Acids, Pkg of 6, 2 Gaskets............. 060082
• Microprocessor-controlled digital signal processing Disposable Gold Electrodes for Amino Acids, Pkg of 24, 8 Gaskets........... 060140
Disposable Gold Electrodes for Carbohydrates, Pkg of 6, 2 Gaskets.......... 060139
• DC amperometry, cyclic voltammetry, and integrated am-
Disposable Gold Electrodes for Carbohydrates, Pkg of 24, 8 Gaskets........ 060216
perometry including 3-D (current, voltage, retention)
Silver Disposable Electrodes, Pack of 6 and two .002" gaskets................. 063003
• Multiple waveforms and integration times to optimize con- Platinum Disposable Electrodes, Pack of 6 and two .002" gaskets............ 064440
ditions for individual analytes Gasket for Disposable Electrode, Pack of 4................................................. 060141
• 3-D display of raw integrated amperometry data similar Gasket, Amperometry Cell............................................................................ 045972
to PDA data display ED Electrode, Au, with Gasket and Polishing Kit......................................... 061749
• Integrated amperometry mode with complete freedom to ED Electrode, AAA, with Gasket and Polishing Kit...................................... 063722
change waveform profile segments ED Electrode, Ag, with Gasket and Polishing Kit......................................... 061755
ED Electrode, Pt, with Gasket and Polishing Kit.......................................... 061751
• Dual-detection configurations (in series or parallel)
ED Electrode, GC, with Gasket and Polishing Kit......................................... 061753
3 mm Gold Working Electrode with Gasket and Polishing Kit.................... 063723
Key Specifications
AAA-Direct Installation Kit........................................................................... 059539
Electronics Type: microprocessor controlled digital signal
processing
Electronic Noise: IPAD <80 pC; DC Amperometry <5 pA
www.dionex.com 87
IC & RFIC Modules
ICS Series Variable Wavelength Key Specifications
Detector Noise: typically <±2.5 x µAU at 254 nm
Drift: <0.1 mAU/hr at 254 nm
Wavelength Accuracy: ±1 nm
Wavelength Bandwidth: 6 nm at 254 nm
Linearity: up to 2.5 AU
Data Collection Rate: up to 100 Hz (multiple wavelength ver-
sion); up to 10 Hz (single wavelength version)
Flow Cell: PEEK, 11 µL volume, 10 mm path length
Lamps: simultaneous tungsten and deuterium
Analog Outputs: two: absorbance, 20-bit resolution, 0–1 Volt
and 0–10 Volt
Dimensions (h × w × d): 15.2 × 44.4 × 50.3 cm,
The high performance ICS-3000 VWD, dual lamp detector is (6.0 × 17.5 × 20 in.)
available in either single or four wavelength capability. PEEK Power Requirements: 85–265 V ac, 47–63 Hz
flow cells are available.
Operating Temperature: 10–35 °C (50–95 °F)
• High signal-to-noise ratio for maximum sensitivity
Operating Humidity Range: 5–80% relative, noncondensing
• Broad wavelength range from 190 to 900 nm
• Deuterium and tungsten lamps for low noise and high Ordering Information
intensity over the full spectral range
• Full control and data collection through Chromeleon soft- Hardware
ware VWD, Single Wavelength, No Flow Cell...................................................... 064377
• USB-based digital data collection for simple installation VWD, 4 Wavelengths, No Flow Cell............................................................. 064654
Digital/Analog Converter for VWD with Cables.......................................... 066349
• Data collection rates up to 100 Hz
VWD Analytical Flow Cell, PEEK, 10 mm, 11 µL.......................................... 066346
• Built-in holmium oxide filter for easy verification of wave-
length accuracy
• Low baseline drift for reliable results
88 www.dionex.com
IC & RFIC Detectors
ICS Series Photodiode Array Detector Key Specifications
Noise: ±10 µAU at 254 nm (flowing water, 2-s rise time);
±15 µAU at 520 nm (flowing water, 2-s rise time)
Drift: < 500 µAU/h
Wavelength Accuracy: ±1 nm, self-calibration with deuterium
lines, verification with built-in holmium oxide filter

Resolution: 1 nm
Linearity: <2 AU
Photodiode Array: 1024 element
Pixel Resolution: 0.7 nm
Optical Resolution: 1.0 nm
Lamps: tungsten and deuterium, simultaneous operation
The ICS-3000 Series Photodiode Array Detector maximizes Analog Outputs: four, 0–3 AU, 1000 mV range
your optical absorbance information by providing full UV-Vis
spectra in high resolution along with an ultra-quiet optical bench Flow Cell Materials: PEEK, fused silica, SST
for excellent detection performance. Flow Cell Pressure Limit: <2 MPa (300 psi) PEEK, <3 MPa
(500 psi) SST
• 1024-element photodiode array for optimum wavelength
resolution Dimensions (h × w × d): 17.4 × 44.4 × 50.3 cm,
(6.8 × 17.5 × 20 in.)
• Broad wavelength range from 190 to 800 nm
• Deuterium and tungsten lamps for low noise and high
intensity over the full spectral range Operating Temperature and Humidity Range: 4–40 °C (40–
104 °F); 5–95% relative, noncondensing
• Full control and data collection through Chromeleon soft-
ware with 3-D option
• USB-based digital data collection for simple installation
• Four analog outputs to support alternate data collection Hardware
• Built-in holmium oxide filter for easy verification of wave- PDA Photodiode Array Detector, No Flow Cell............................................. 064447
length accuracy Absorbance Cell, PEEK, 13 µL....................................................................... 056346
• Low baseline drift for excellent reliability and reproduc- Absorbance Cell, Semi Micro, PEEK, 3 µL, 9 mm Path Length.................... 064169
ibility Absorbance Cell, Semi Prep, PEEK, 0.7 µL, 0.4 mm Path Length................ 064167
Absorbance Cell, SST, PDA-100.................................................................... 056126
Absorbance Cell, Semi Micro, SST, 3 µL, 9 mm Path Length...................... 064168
Software
Chromeleon Option for PDA Full Spectrum Data Acquisition...................... 060728
www.dionex.com 89
IC & RFIC Modules
Postcolumn Reaction Systems PC10 Postcolumn Delivery System
The PC10 Postcolumn Pneumatic Delivery System ensures a
constant, pulseless flow of reagent. It assures optimal flow by
applying constant pressure, eliminating the need for a pump and
its associated moving parts, labor, and expense.
The PC10 Automation Kit option supports automation of the

PC10. The kit includes a two-way valve to shut off flow from
the PC10 to the analytical system, and a solenoid for valve
control. Do not use this kit if configuring an ICS-3000. Use
solenoids valves with the DC instead.
Accessories
Postcolumn reagent chemistries, as delivered by the PC10 Post- PC10 Postcolumn Pneumatic Delivery Pkg, 4 mm........................................ 050601
column Pneumatic Delivery System, often can extend detection PC10 Postcolumn Pneumatic Delivery Pkg, 2 mm........................................ 053591
limits for ions that would otherwise exhibit detection sensitivity. PC10 Pneumatic Controller........................................................................... 043903
Pressurizable Chamber.................................................................................. 037460
PC10 Reagent Organizer............................................................................... 050602
Related Literature Knitted Reaction Coil, 375 µL, Unpotted...................................................... 043700
For detailed specifications and applications, see the following Knitted Reaction Coil, (For 2 mm system, 125 µL)........................................ 053640
PDF documents under Literature on www.dionex.com. PC10 Automation Kit..................................................................................... 050603
Product Data Sheets
PC10 Postcolumn Delivery System
90 www.dionex.com
Process Analytical Technology
Process Analytical Systems & Software 93
DX-800 Process Analyzers.................................... 94


Chromeleon PA Software..................................... 94
www.dionex.com 91

92
www.dionex.com
Process Analytical Systems & Software
Improve your process by improving your process
monitoring
Dionex Process Analytical Systems are used worldwide to pro-
vide analysis results on-line. Reduce reporting delays, improve
process throughput and efficiencies, and reduce process down-
time. Dionex has over 20 years of experience providing process
analytical (PAT) technology for more comprehensive, precise,
accurate, and timely information in a wide range of industries.

DX-800 Process Analyzers: DX-800 Analyzers are configured
• Characterize your samples completely with multicompo- with various analytical modules to perform continuous on-line
nent analysis. analysis
• Save sample collection time with automated sampling at Chromeleon PA Software: Expanded capabilities for configu-
multiple points. ration, multi-system control, conditional logic, and industrial
connectivity for process systems
• Improve your process control with more precise results.
• See more analytes with unique detection capabilities.
• Choose a system configuration that best meets your needs.
www.dionex.com 93
Process Analytical Systems & Software
DX-800 Process Analyzers Related Literature
Designed for reliability, ease of operation, For detailed specifications and applications, see the following
reproducibility, accuracy, and regulatory compliance PDF documents under Literature on www.dionex.com.
The DX-800 Process Analyzer’s IC and HPLC capabilities

allow determinations of species that cannot be determined with Product Brochures
other process analytical techniques. With the DX-800, multi-
DX-800 Process Analyzer Brochure
component characterization of a sample can be performed in
a single analysis, and multiple samples can be scheduled for
automatic analysis. Ordering Information
• Configured with one or more systems, all analyzing the Please contact your Dionex representative for a customized
same sample in parallel product offer.
• Can handle 1–21 samples to be analyzed in series through
software control
Chromeleon PA Software
Each system is configured with an appropriate analytical pump,
Advanced software for PAT applications
detector, accessories, and set of analysis columns. These com-
ponents are determined by the specific analysis being run on Chromeleon PA 6.60 provides DX-800 analyzer control, data
the system. The pumps and detectors are process versions of the acquisition, and data reporting with a versatile software inter-
GP50, IP25, CD25A, ED50A, and AD25 based on the proven face for viewing analyzer status, handling alarms, and reporting
and reliable ICS-2500 product line. results.
All instrumentation is software controlled. All raw data is stored • Based on core Chromeleon client-server functionality.
in a secure database with audit trail support. Dionex-designed
• Additional Analyzer Client provides specialized configura-
Chromeleon PA software provides configuration, control,
tion and control capabilities.
analyzer and system status, results display and trending, and
user-defined results reporting.
Ordering information is provided for reference. Contact your
local representative for assistance in configuring software for
your installation.
Accessories
Chromeleon-PA Base Server and Client Bundle........................................... 062827
Class 3 Timebase (for full control of competing low-priced LCs)................ 060724
Chromeleon-PA Analyzer Client License...................................................... 062804
Chromeleon Client Option - GLP Compliance............................................... 060731
Chromeleon Server Option - OPC Driver....................................................... 062811
94 www.dionex.com
Mass Spectrometry
MS Instruments 97
MS Systems and Modules................................... 98

MSQ Plus Mass Spectrometer......................................... 98
AXP-MS Auxiliary Pump for MS...................................... 99
MSQ18LA Nitrogen Gas Generator............................... 100
Mass Spectrometry
www.dionex.com 95

Mass Spectrometry
96 www.dionex.com
MS Instruments
Single-point control and automation for improved
ease-of-use in LC/MS and IC/MS
Dionex provides advanced integrated IC/MS and LC/MS solu-
tions with superior ease-of-use and modest price and space
requirements. UltiMate 3000 System Wellness technology and
automatic MS calibration allow continuous operation with
minimal maintenance. ICS-3000 and ICS-2000 RFIC systems
automatically remove mobile phase ions for effort-free transi-
tion to MS detection. MS Systems and Modules: MSQ Plus Mass Spectrometer;
MSQ18LA nitrogen gas generator; AXP-MS digital auxiliary
• MSQ Plus, the smallest and most sensitive single quadru- pump
pole on the market for LC and IC
Mass Spectrometry
• Self-cleaning ion source for low-maintenance operation
• Chromeleon software for single-point method setup, instru-
ment control and data management
• Compatible with existing IC and LC methods
• The complete system includes the MSQ Plus, PC datasys-
tem, ESI and APCI probe inlets, and vaccum system
You no longer need two software packages to operate your LC/

MS system. Chromeleon LC/MS software provides single-soft-
ware method setup and instrument control, powerful UV, con-
ductivity, and MS data analysis, and fully integrated reporting.
www.dionex.com 97
MS Instruments
MS Systems and Modules MSQ Plus Mass Spectrometer
Mass Spectrometry
The MSQ Plus features a newly designed self-cleaning Atmo- The MSQ Plus Mass Spectrometer can be used in a wide range
spheric Pressure Ionization (API) source for outstanding sen- of applications and methodologies in both HPLC and IC. Only
sitivity and noise reduction. Compatible with existing LC and 12 inches wide, the MSQ Plus is by far the most compact mass
IC methods, the MSQ Plus is more sensitive, rugged, powerful, spectrometer on the market today. It provides universal detec-
easier to use, and smaller than any other instrument of its kind. tion and characterization of analytes in the 17–2000 m/z range.
• New M Path™ source design virtually eliminates neutral

noise and background
Related Literature
• Unique FastLoc™ probes provide simple and rapid
For detailed specifications and applications, see the following changes for both ESI and APCI modes
• All features fully supported in Chromeleon Chromatogra-
phy Management software
Product Data Sheets
• Full scan and Selected Ion Monitoring allow rapid screen
MSQ Plus Mass Spectrometric Detector and target compound analysis
MSQ18LA Nitrogen Gas Generator • Quadrupole analyzer maximizes resolution and provides
high stability and minimal drift
AXP-MS Auxiliary Pump for Mass Spectrometry Data Sheet
• Enhanced low-mass response for analytes below 60 m/z
• All new Ion Bright™ detector system eliminates neutral
noise, extending the dynamic range
• Advanced autotuning wizard includes full system optimiza-
tion and mass scale calibration
The MSQ Plus features a high-sensitivity, self-cleaning ESI/

APCI ionization source that requires no mechanical adjustment
or electrostatic focusing for sample optimization. The compact
source design is optimized for a wide range of flow rates and
supports a wide range of chromatographic buffers.
Note: Sensitivity specifications below are based on loop

injections at a flow of 1 mL/min in SIM mode. Noise is RMS
after appropriate smoothing.
98 www.dionex.com
MS Systems and Modules
Key Specifications AXP-MS Auxiliary Pump for MS
Power Requirements: 230 V ac 13A power outlets (for rotary
pump and MSQ); 230 or 115 V ac (as required) for PC and
monitor and additional hardware
Gas Requirements: MSQ requires a supply of high-purity
(>99%) nitrogen capable of supplying 12 L/min at 75 psi (5
bar).
Dimensions (h × w × d): 530 × 300 × 710 mm
(21 × 12 × 28 in.)
Weight: 60 kg (132 lb)
Ionization Modes (supplied as standard): Electrospray (ESI),
Atmospheric Pressure Chemical Ionization (APCI)
Mass Spectrometry
Range: 17–2000 m/z
Sensitivity, positive ion ESI: 50 pg injection (10 µL × 5 pg/µL)
of erythromycin: 1000:1 S/N The AXP-MS single-piston pump uses a digital stepper drive
and rapid refill to deliver precise and accurate flow. An internal
Sensitivity, negative ion ESI: 20 pg loop injection (10 µL × 2 pg/ transducer provides pressure display and monitoring capabil-
µL) of p-nitrophenol: 500:1 S/N ity. The AXP-MS is a high-quality auxiliary pump for MS
support or general metering applications. It is also useful as a
Sensitivity, positive ion APCI: 50 pg injection (10 µL × 5 pg/µL)
flow source when other pumping is not available or would be
of erythromycin: 200:1 S/N
inconvenient.
Sensitivty, negative ion APCI: 20 pg injection (10 µL × 2 pg/µL)
of p-nitrophenol: 50:1 S/N • Wide flow range
• Internal pulse damper
• Fully IC and biocompatible
Hardware • Positive seal-washing standard to extend seal life by remov-

ing salts and wetting the seal
MSQ Plus Mass Spectrometric Detector, Computer, and Software........... 063116
• Easy maintenance
Accessories • Flexible, advanced Chromeleon control (version 6.8 or
Odor Removal Filters (5) for MS Vacuum Pump Oil Trap............................. 063142 later)
Kit for MSQ PM and Annual Maintenance.................................................. 061494 • Integrated injection valve with a purge/priming port for
Kit for API Probe Maintenance..................................................................... 061495 precise flow or loop injections
Perchlorate O-18 Internal Standard, 1 mg/L, 10 mL.................................... 062923
Software
Chromeleon Mass Spec Software Control Option....................................... 060726
www.dionex.com 99
MS Instruments
Key Specifications • Minimal servicing requirements
Rated Pressure: 2500 psi • Internal air compressor
Flow Rates: 0.01–1.00 mL/min • CE approved
Flow Accuracy: 3% throughout flow range • Energy efficient
Pressure Pulsation: 2% peak-to-peak at 100 psi, 1 mL/min with The quiet-running MSQ18LA uses membrane technology to
in-line pulse damper selectively remove moisture, oxygen, and other gasses for clean,
Flow Rate Precision: 0.5% at calibration pressure dry, phthalate-free nitrogen at a max flow of 18 L/min. The
internal oil-free air compressor ensures years of operation with
Control: full serial/USB control with Chromeleon 6.8 or no fall-off in purity or performance. Its PLC features a total run
greater; generic serial driver control (no status feedback); front hour count for the compressor, and tracks time to next service.
panel and relay control
Dimensions (h × w × d): 6.5 × 6.5 × 10 in Note: The MSQ18LA is for the operation of a single MSQ MS.
Mass Spectrometry
Weight: 15 lbs
Key Specifications
Power: 120/230 V ac; 50/60 Hz
Flow Rate: 18 L/min
Materials: PEEK, ceramic, and inert polymers
Outlet Pressure: 100 psi (7 bar)
Ordering Information Internal Air Compressor: yes
Particles >0.01 µm: none
Hardware
Electrical Requirements: 220 V 50/60 Hz 4A
AXP-MS Auxiliary Pump................................................................................ 060684
Outlet Port: 6 mm
MSQ18LA Nitrogen Gas Generator Dimensions: 35 × 17 × 16 ins/89 × 43 × 41 cms

Weight: 90 lbs (40 kg)
Product Certificate: CE approved; EN61010:1993;
EN60204:1992; EN50116:1996
Hardware
MSQ18LA Nitrogen Generator for MS......................................................... 068126
Filter Maintenance Kit for MSQ18LA........................................................... 068349
With constant pressure, flow, and purity, the MSQ18LA gives

you complete independence from cylinder changes, gas costs,
and associated administration charges. In addition, you will
have the maximum safety, convenience, and productivity for
your instrument.
• Constant gas supply

• Up to 15 times more economical than cylinders
• Simple installation
100 www.dionex.com
Sample Extraction
ASE Accelerated Solvent Extractors 103
ASE Systems....................................................... 104

Related Literature.............................................................. 104
Ordering Information........................................................ 104
ASE Parts & Accessories 105
ASE Starter Kits................................................... 106

ASE Heat Exchanger........................................... 106
Sample Extraction
ASE Prep DE and CR........................................... 106

ASE Cells.............................................................. 106

Dionium ASE Cells............................................................. 106
Stainless Steel ASE Cells................................................. 107
ASE Cell Parts.................................................................... 107
Filters and Thimbles........................................... 108

ASE Filters........................................................................... 108
ASE Thimbles..................................................................... 108
Vials and Bottles.................................................. 108

ASE Vials and Bottles....................................................... 108
www.dionex.com 101

Sample Extraction
102 www.dionex.com
ASE Accelerated Solvent Extractors
Better extractions in less time, using less solvent
ASE systems use a patented technique for the extraction of solid
and semisolid samples using common solvents at elevated tem-
perature and pressure. The ASE 150 and 350 feature pH-hard-
ened pathways with Dionium components to support extraction
of acidic or alkaline matrices and combine pretreatment, solvent
extraction, and cleanup in one step. Dionium is zirconium that
has undergone a proprietary hardening process that makes it
inert to chemical attack by acids and bases at elevated tempera- ASE Systems: ASE uses solvents at elevated temperatures and
tures. pressure to extract organic and ionic compounds from solid
samples
• US EPA Method 3545A
• CLP SOW OLM 0.42
• ASTM Standard practice D7210
Sample Extraction
• Chinese method GB/T 19649-2005
• German Method L00.00-34
ASE is dramatically faster than Soxhlet, sonication, and other

extraction methods, and uses significantly less solvent and labor.
ASE methods are accepted and established in the environmen-
tal, pharmaceutical, foods, polymers and consumer product
industries. ASE method are accepted and used by goverment
agencies worldwide.
www.dionex.com 103
ASE Accelerated Solvent Extractors
ASE Systems Related Literature
Two new solvent extraction systems with pH- For detailed specifications and applications, see the following
hardened Dionium components PDF documents under Literature on www.dionex.com.
Dionex offers two solvent extraction systems. The ASE 150 is

an entry-level system with a single extraction cell, for labora- Product Brochures
tories with modest throughput. The ASE 350 is a sequential
ASE Series Accelerated Solvent Extractors Brochure
extraction system capable of automated extraction of up to
24 samples. Both systems feature chemically-inert Dionium Product Datasheets
components that allow the extraction of acid- or base-pretreated
samples. ASE 150 Accelerated Solvent Extractor
ASE 350 Accelerated Solvent Extractor
Application Notes
AN 356: Determination of Perchlorate in Vegetation Samples

Using Accelerated Solvent Extraction (ASE) and Ion Chroma-
tography
Sample Extraction
AN 357: Extraction of Phenolic Acids from Plant Tissue Using

ASE
AN 358: Extraction and Cleanup of Acrylamide in Complex

Matrices Using Accelerated Solvent Extraction (ASE) followed
by Liquid Chromatography Tandem Mass Spectrometry (LC-
MS/MS)
AN 359: Extraction of Contaminants, Pollutants, and Poisons

The ASE 150 Accelerated Solvent Extractor. from Animal Tissue Using Accelerated Solvent Extraction
(ASE)
AN 361: Extraction of Total Fat from Food Samples After Acid

Hydrolysis Using Accelerated Solvent Extraction (ASE) with
GC-MS Analysis
Hardware
ASE 150 Accelerated Solvent Extractor....................................................... 066400
This system does not include extraction cells. Installation is not covered.
Installation is P/N 057798.
ASE 350 Accelerated Solvent Extractor....................................................... 066050
The ASE 350 supports automated extraction of up to 24 samples with This system does not include extraction cells. Installation is included.
varying cell sizes.
104 www.dionex.com
ASE Parts & Accessories
ASE accessories to improve productivity and reduce
the need for sample handling
Dionex offers ASE part and accessories to maintain high
productivity in sample extraction. All are desgined to meet the
unqiue specifications of ASE instrumentation, and manufactured
to meet the quality requirements of modern analytical laborato-
ries.
ASE Starter Kits: Everything you need to get started with your
ASE system.
ASE Heat Exchanger: Cools solvent prior to collection.
ASE Prep DE and CR: Better extractions with ASE Prep DE
dipersent and drying agent, or Prep CR cation exchange resin to
absorb strong mineral acids.
Sample Extraction
ASE Cells: Extraction cells and cell parts for ASE systems.
Filters and Thimbles: Filters and thimbles for uses in ASE
extraction cells.
Vials and Bottles: Collection vials for ASE systems. Includes
lids and septa.
www.dionex.com 105
ASE Starter Kits ASE Prep DE and CR
Everything you need to start using ASE Improve your ASE extractions
ASE Starter Kits are customized to match your instrument and ASE Prep DE is pelletized diatomaceous earth used as a diper-
cell size requirements include rinse tubes, funnels, filters, and sent and drying agent with wet samples.
vials designed to match various ASE cell sizes the 34 mL and
small-volume kits for ASE 350 include 60 mL collection vial ASE Prep CR is a Dionex-proprietary cation exchange resin
inserts. The kits also include PEEK seals and o-rings for cell (NA form) designed to absorb strong mineral acids. Mix with
maintenence. acid hydrolyzed samples for lipid determination using the ASE
150 or 350 system.
ASE Prep CR is a Dionex-proprietary cation exchange resin
(NA form) designed to absorb strong mineral acids. Mix with
acid hydrolyzed samples for lipid determination using the ASE Ordering Information
150 or 350.
Note: Contact your local Dionex sales representative to Accessories

confirm part numbers. ASE prep DE Diatomaceous Earth Dispersant for ASE, 1 kg Bottle............ 062819
1 kg container; for use with all ASE systems
Sample Extraction
ASE Prep CR Resin for Neutralization of Mineral Acid, 500 grams.............. 80024
ASE Cells
ASE 150 and 350 Starter Kits
ASE 150 Startup Kit, Small Volume.............................................................. 068250
ASE 150 Startup Kit, 34 mL........................................................................... 068251
ASE 150 Startup Kit, 100 mL......................................................................... 068253
ASE 350 Startup Kit, Small Volume.............................................................. 068254
ASE 350 Startup Kit, Large Volume.............................................................. 068256
ASE Heat Exchanger

The ASE Heat Exchanger uses a water jacket to cool solvent as
it leaves the extraction cells. It is useful for high-temperature ex-
tractions (above 150 ˚C) to minimize solvent or volatile analyte
loss.
Replacement cells and cell parts for ASE systems are provided
in stainless steel (all ASE systems) and Dionium (ASE 150 and
Note: Contact your local Dionex sales representative to 350 only). Use the Dionium cells for samples that require treat-
confirm part numbers. ment with acid or base.
Ordering Information Dionium ASE Cells
ASE Heat Exchanger Ordering Information

Heat Exchanger for ASE 150 or 350............................................................. 068247
Dionium Cell Kits for ASE 150 and 350
66 mL Dionium Extraction Cell Kit, 1 cell for ASE 150 or 350..................... 068102
100 mL Dionium Extraction Cell Kit,1 cell for ASE 150 or 350.................... 068103
66 mL Dionium Extraction Cell Kit, 3 cell for ASE 150 or 350..................... 068104
100 mL Dionium Extraction Cell Kit, 3 cell for ASE 150 or 350................... 068105
106 www.dionex.com
ASE Starter Kits
Stainless Steel ASE Cells ASE Cell Parts
O-rings, PEEK seals and frits require periodic replacement to
ensure a proper seal and maximize extraction efficiency.
Stainless Steel Cell Kits for ASE 150 and 350

1 mL Stainless Steel Extraction Cell Kit, Pkg of 6........................................ 068095
5 mL Stainless Steel Extraction Cell Kit, Pkg of 6........................................ 068096
Cell Parts for ASE 100, 150, 300 or 350
10 mL Stainless Steel Extraction Cell Kit, Pkg of 6...................................... 068097
22 mL Stainless Steel Extraction Cell Kit, Pkg of 6...................................... 068098 100 mL Extraction Cell Body......................................................................... 056693
34 mL Stainless Steel Extraction Cell Kit, Pkg of 6...................................... 068099 66mL Extraction Cell Body............................................................................ 056696
66 mL Stainless Steel Extraction Cell Kit, Pkg of 6...................................... 068100 34 mL Extraction Cell Body........................................................................... 056646
100 mL Stainless Steel Extraction Cell Kit, Pkg of 6.................................... 068101 Frits, 10µm SST, ASE Cells, Pkg of 50........................................................... 056775
1 mL Stainless Steel Extraction Cell Kit, 1 cell............................................ 068085 ASE 300/100 PEEK Seals, Pkg of 50............................................................. 061687
5 mL Stainless Steel Extraction Cell Kit, 1 cell............................................ 068086 Vesple Seals for ASE 300/100 Cell Caps, Pkg of 50.................................... 056776
10 mL Stainless Steel Extraction Cell Kit, 1 cell.......................................... 068087 Vesple Seals for ASE 300/100 Cell Caps, Pkg of 10.................................... 056777
22 mL Stainless Steel Extraction Cell Kit, 1 cell.......................................... 068088 Snap Ring for ASE Cell Caps, Pkg of 10....................................................... 056778
Sample Extraction
34 mL Stainless Steel Extraction Cell Kit, 1 cell.......................................... 068089 O-Rings, Teflon, ASE Cell Cap, Pkg of 50...................................................... 049457
66 mL Stainless Steel Extraction Cell Kit, 1 cell.......................................... 068090 O-Rings, Viton, ASE Cell Caps, Pkg of 50..................................................... 056325
100 mL Stainless Steel Extraction Cell Kit, 1 cell........................................ 068091

Cell Parts for ASE 150 or 350
Stainless Steel Cell Kits for ASE 100 and ASE 300 Short Rinse Tube for Use with ASE 150....................................................... 060174
10 mL Extraction Cell Kit, Pkg of 6................................................................ 060074 Medium Rinse Tube for Use with ASE 150.................................................. 060175
34 mL Extraction Cell Kit, Pkg of 6................................................................ 060075 Long Rinse Tube for Use with ASE 150........................................................ 060176
66 mL Extraction Cell Kit, Pkg of 6................................................................ 060076 Endcap, package of 2, Dionium, for ASE 350............................................... 068107
100 mL Extraction Cell Kit, Pkg of 6.............................................................. 060077

Cell Parts for ASE 200
10 mL Extraction Cell, 1 cell.......................................................................... 060070
34 mL Extraction Cell, 1 cell.......................................................................... 060071 11 mL Extraction Cell Body for ASE 200....................................................... 048820
66 mL Extraction Cell, 1 cell.......................................................................... 060072 22 mL Extraction Cell Body for ASE 200....................................................... 048821
100 mL Extraction Cell, 1 cell........................................................................ 060073 33 mL Extraction Cell Body for ASE 200....................................................... 048822
Includes seals, frits and o-rings
Stainless Steel Cell Kits for ASE 200 1 mL Extraction Cell Body for ASE 200......................................................... 054973
5 mL Extraction Cell Body for ASE 200......................................................... 054974
11 mL Extraction Cell Kit, Pkg of 6 for ASE 200........................................... 049560
Extraction Cell End Caps, Pkg of 2, Includes Frits, Seals for ASE 200........ 049450
22 mL Extraction Cell Kit, Pkg of 6 for ASE 200........................................... 049561
Includes seals, frits and o-rings
33 mL Extraction Cell Kit, Pkg of 6................................................................ 049562
Frits, 10µm SST, ASE Cells, Pkg of 50 for ASE 200 cells............................. 049453
1 mL Extraction Cell, Pkg of 6 for ASE 200................................................... 055421
ASE 200 PEEK Seals, Pkg of 50.................................................................... 049454
5 mL Extraction Cell, Pkg of 6 for ASE 200................................................... 055422
Snap Ring, ASE 200 Cell Cap, Pkg of 10....................................................... 049456
1 mL Extraction Cell, Single for ASE 200...................................................... 055815
5 mL Extraction Cell, Single for ASE 200...................................................... 055817
11 mL Extraction Cell, Single for ASE 200................................................... 048765
www.dionex.com 107
Filters and Thimbles Vials and Bottles
Use filters and thimbles in the extraction cells when extracting
complex matrices to prevent plugging and maximize separation
of the solvent extract from the matrix.
ASE Filters
Cellulose and glass fiber filters for use on the ASE systems
specified below.
Filters for ASE 150 and 350

FLTR,27MM,GF/B,ASE350,PK/100............................................................... 068092
FLTR,27MM,CELLULOSE,ASE350,PK/100.................................................... 068093 Collection vessels for ASE systems are available in 40, 60, and
Sample Extraction
250 mL sizes. Collection vials and bottles come with lids and
Filter, ASE100, 10mL Cell.............................................................................. 060941
solvent resistant (TFE) septa. Not compatible with the ASE 150
or 350. 40 mL and 60 mL graduated are only compatible with
Filters for ASE 100, 150, 300, or 350
the ASE 200. 60 mL collection vials are not compatible with the
Filters, Cellulose 30 mm, for 34, 66 or 100 mL Cells, Pkg of 100................ 056780 ASE 100.
Filters, Glass Fiber, 30 mm, for 34, 66, or 100 mL Cell, Pkg 0f 100............. 056781
Filter, ASE100, 10 mL Cell............................................................................. 060941
Filters, Cellulose, for 5 mL Extraction Cells, Pkg of 100............................... 055399 ASE Vials and Bottles
Filters for ASE 200 Only Ordering Information

Filters, Cellulose for 11, 22, or 33 mL Cell, Pkg of 100 for ASE 200............ 049458
Filters, Glass Fiber, for 11, 22 or 33 mL Cell, Pkg of 100 for ASE 200......... 047017 Collection Bottles
Filters, Cellulose, for 1 mL Extraction Cell, pkg of 100................................ 055398 250 mL Clear Collection Bottles for ASE 100, 150 or 350, Pkg/12, VOC
Filters, Cellulose for 5 mL Extraction Cell, pkg of 100................................. 055399 Cert................................................................................................................. 056284
Can be used with ASE 100, 150, 300, or 350.
ASE Thimbles Septa

Thimbles for use on ASE 200 systems. Ultra Low Bleed Septa, Pkg of 72................................................................. 055395
Can be used with all ASE systems.
Collection Vials for ASE 150, 200, and 350
Thimbles for ASE 200 Only 40 mL clear Collection Vials for ASE 200, l, Pkg/72..................................... 048783
60 mL Clear Collection Vials for ASE 150, 200 or 350, Clear, Pkg/72......... 048784
Thimbles, Cellulose, 11 mL ASE 200 Cell, Pkg of 25................................... 055708
40 mL Amber Collection Vials for ASE 200, l, Pkg/72.................................. 048780
Thimbles, Cellulose, 22 mL ASE 200 Cell, Pkg of 25................................... 055999
60 mL clear Collection Vials for ASE 150, 200 or 350, Amber, Pkg/72....... 048781
Graduated Vials for ASE 150, 200, and 350

Conc.Vial with Spacer (Graduated), 40 mL, Pkg of 6.................................... 055441
Conc.Vial (Graduated), 40 mL, Pkg of 6........................................................ 055442
108 www.dionex.com
Software
System Control Software 111
Chromeleon......................................................... 112
Single Workstation Configuration.................................. 112
Chromeleon Networked Configuration......................... 113
Chromeleon 6.8 Extension Pack..................................... 113
Chromeleon Xpress.......................................................... 114
Virtual Column Separation Simulator............................ 114
DCMSLink . ............................................................. 115
Software
www.dionex.com 109

Software
110 www.dionex.com
System Control Software
Discover the world’s most complete chromatography
software for HPLC, GC, and IC.
Chromeleon controls over 300 different HPLC, GC, and IC
instruments from more than 30 manufacturers. Dynamic data
processing and superior reporting tools calculate and publish
results in just a few minutes, providing measurable productivity
gains for your laboratory. Chromeleon offers a complete suite
of features for 21 CFR Part 11 compliance, and can be quickly
installed into any laboratory environment. Chromeleon: State-of-the-art instrument control, data process-
ing and reporting tools, and regulatory compliance tools.
• Controls more than 300 HPLC, LC, and IC instruments
DCMSLink: Direct control of Dionex instruments from MS soft-
• Controls all Dionex chromatographs, Agilent ware by ABI/Sciex, Thermo, and more.
1100/1200/6890, Waters Alliance, and many more
• Reduces data processing and reporting times by more than
70%
• Gets you quickly from data to knowledge with powerful
query, trend, and reporting tools
• Provides complete set of tools for 21 CFR Part 11
Software
compliance
DCMSLink provides direct control of Dionex instruments from

Mass Spectrometry software from ABI/Sciex, Thermo Fisher
Scientific, and Bruker Daltonics.
Note: For more information and ordering details, please

contact your local Dionex representative.
www.dionex.com 111
Chromeleon Application Notes
AN 174: Calculating Instrument Utilization Using Chromeleon
Technnical Notes
TN 54: Using Chromeleon Chromatography Management Soft-

ware to Comply with 21 CFR Part 11
TN 57: Automated System Suitability Testing with Chromeleon
TN 65: Using Chromeleon in a Networked Environment
TN 67: Instrument Control and Data Acquisition with Chrome-

leon
TN 70: Data Reporting in Chromeleon
TN 703: Using the Chromeleon SmartStart Wizard
Chromeleon controls more than 300 different HPLC, GC, and

Single Workstation Configuration
IC instruments from more than 30 manufacturers. Dynamic
data processing and superior reporting tools help you calculate
and publish results fast, providing significant and measurable
productivity gains.
Software
• Stand-alone configuration for laboratories with few instru-

ments
• Networked configuration for central data storage and man-
agement
• Productivity tools for method validation and related sub-
stances testing
• Xpress configuration for using Dionex instruments with
other chromatography data systems
Chromeleon offers a complete suite of features for 21 CFR Part

11 compliance and can be quickly installed into any laboratory
A Chromeleon single workstation configuration provides
environment.
everything you need to control one instrument. This configura-
tion provides all control, data processing and reporting, and
data storage options, and is expandable to support additional
Related Literature instruments.
For detailed specifications and applications, see the following • Easy installation and configuration for immediate use
• Remote instrument control and monitoring capability avail-
able
Product Brochures
• Full range of 21 CFR Part 11 compliance tools available
Chromeleon 6 - Chromatography Management System Bro-
chure • Seamless scaleability to a networked configuration
Chromeleon Chromatography Management System for Ion

Chromatography Brochure
Virtual Column Brochure
Product Data Sheets
Computer Requirements for Chromeleon
112 www.dionex.com
Chromeleon
Chromeleon Networked Configuration Chromeleon 6.8 Extension Pack
A Chromeleon networked configuration makes all Chromeleon The Chromeleon 6.8 Extension pack provides tools that can
capabilities available from anywhere on your network. Start calculate and report the results from ICH Method validation
and monitor chromatography runs from your office, review data experiments and from Related Substances experiments in just a
from remote locations, and manage all instruments and data few minutes.
from a central location.
• Calculate and report all validation results in under 5 min-
Software
• Easy installation and configuration for immediate use utes
• Network failure protection for data security • Instantly calculate related substances results
• Centralized data storage for easy searching and data com- • Instantly assess all results against your specifications
parison
• Sign results electronically
• Centralized data management, even for sites in different
• Print reports anytime, or export them in pdf format
countries
• Full compatibility with SQL Server and Oracle databases
• Centralized user account management tools
• Full range of 21 CFR Part 11 compliance tools
• Full compatibility with Citrix and Windows Terminal Serv-
ers
www.dionex.com 113
Chromeleon Xpress Virtual Column Separation Simulator
Chromeleon Xpress provides an optimized control interface for Determine the best column and separation conditions for any IC
Dionex instruments that are used with data acquisition software application in just a few minutes. Virtual Column eliminates the
from a different company. risks and hassles of optimizing IC separations by modeling their
behavior using pre-acquired, Dionex-validated data.
• Intuitive graphical controls for Dionex instruments
• Optimize your separations to get better resolution in less
• Real-time display of chromatograms and operating status
Software
time
• Easy access to special features like wellness counters and
• Separate complex mixtures with no laboratory work
qualification status monitoring
• Quickly create instrument control programs and quantita-
• Choice of computer platforms - laptop, tablet, or desktop
tion methods for the optimized separation
If you have to use a chromatography data system other than
Virtual Column can also model separations under gradient
Chromeleon, such as Chemstation or Empower, you can still use
conditions, demonstrating how easy it is to get well-resolved,
the advanced UltiMate 3000 HPLCs and ICS-3000 ICs from
sharper peaks in less time.
Dionex. Chromeleon Xpress provides high-level instrument
control and sequence creation tools for operating these instru-
ments.
114 www.dionex.com
DCMSLink
DCMSLink
Direct control of Dionex instruments from MS
software by ABI/Sciex, Thermo, and more.
DCMSLink provides direct control of Dionex instruments from
mass spectrometry software from ABI/Sciex, Thermo Fisher
Scientific, and Bruker Daltonics. This allows the use of the in-
novative UltiMate 3000 HPLC and ICS-3000 IC products as a
front end to some of the most popular mass spectrometry instru-
ments on the market today.
• DCMSLink for Analyst

• DCMSLink for HyStar
• DCMSLink for Xcalibur Control interface for the Dionex UltiMate 3000.
DCMSLink brings additional tools to other MS software, includ-

ing graphical control interfaces for the Dionex instruments.
Software
Easy access to system wellness counters.
DCMSLink provides control of Dionex chromatography devices through

MS software from ABI/Sciex, Thermo Fisher Scientifc, and Bruker
Daltonics.
Control interface for the Dionex ICS-3000.
www.dionex.com 115
Software
116 www.dionex.com
LC Columns
Reversed-Phase LC Columns 119 Specialty LC Columns 133
Acclaim 120 C18.................................................. 120 Acclaim Organic Acid......................................... 134

Wide Range of Applications........................................... 120 Acclaim OA Column Applications.................................. 134
Related Literature.............................................................. 121 Related Literature.............................................................. 135
Ordering Information........................................................ 121 Ordering Information........................................................ 135
Acclaim 120 C8.................................................... 122 Acclaim Surfactant.............................................. 136

Related Literature.............................................................. 123 Broad Range of Applications.......................................... 136
Ordering Information........................................................ 123 Related Literature.............................................................. 137
Acclaim PolarAdvantage.................................... 124
Applications........................................................................ 124 Acclaim Explosives............................................. 138
Acclaim PolarAdvantage II................................. 126 Acclaim Mixed-Mode HILIC-1............................ 140

Highly Stable Under Wide pH Range............................ 126 Wide Range of Applications........................................... 140
LC Columns
Acclaim Rapid Separation LC............................ 128 Acclaim Mixed-Mode WAX-1............................. 142
Related Literature.............................................................. 129 Multi-Mode Separation Mechanism............................. 142
IonPac NS1........................................................... 130
Key Applications................................................................ 130 Acclaim Mixed-Mode WCX-1............................. 144
Related Literature.............................................................. 131 Broad Range of Applications.......................................... 144
OmniPac............................................................... 146
Wide Range of Applications........................................... 146
www.dionex.com 117

LC Columns
118 www.dionex.com
Reversed-Phase LC Columns
Reversed-phase silica and polymer columns for high-
efficiency separations
Acclaim columns meet the high standards set by modern HPLC
and LC/MS methods. The low metal contamination of the raw
silica and dense bonding chemistry provide exceptionally high
column efficiencies, symmetrical peaks for basic analytes, and
low bleed for LC/MS applications. The uncompromising per-
formance of these columns ensures outstanding peak shape for
basic, acidic, hydrophilic, and hydrophobic analytes in a single Acclaim 120 C18: High-density, monolayer C18 reversed-phase
run. columns for exceptional resolution in a variety of applications.
• Ultrapure silica substrate Acclaim 120 C8: High-density monolayer C8 reversed-phase

column.
• Optimized proprietary high-density bonding process, and
double endcapping Acclaim PolarAdvantage: Sulfonamide-embedded column for
separating a wide variety of polar and nonpolar analytes.
• Reliable manufacturing process with thorough testing
Acclaim PolarAdvantage II: Amide-embedded reversed-phase
• LC/MS compatible columns with enhanced hydrolytic stability.
• IonPac NS1 for determination of high molecular weight Acclaim Rapid Separation LC: Fast, high-resolution separa-
ionic analytes. tions with Dionex RSLC columns and the UltiMate 3000 RSLC
system.
Acclaim columns include the 120Å C18, C8, Polar Advantage
(PA) and Polar Advantage II (PA2). They are available with IonPac NS1: Polymeric based, reversed-phase column ideal for
LC Columns
3 and 5 µm packing, and support a wide variety of applications ion-pair or ion-suppression separations of hydrophobic, ioniz-
in pharmaceutical, environmental, food testing, and product- able compounds.
quality testing. The IonPac NS1 is a reversed-phase column
based on a rugged DVB copolymer, available in both 5 and
10 µm particle sizes.
Note: See the Acclaim Library of applications on

www.dionex.com under Literature, Application Notes.
www.dionex.com 119
Acclaim 120 C18 Caffeine Analogs
High performance reversed-phase columns for the
350
separation of small molecules 1
Column: Acclaim 120 5 µm C18
Dimensions: 4.6 × 150 mm
Mobile Phase A: 50 mM sodium acetate
Mobile Phase B: Acetonitrile
The Acclaim 120 columns are designed for high resolution Mobile Phase C: Methanol
reversed-phase separations. The very high surface coverage and Composition: (A) 6%, (B) 8%, (C) 6%
Flow rate: 1.0 mL/min
very low metal content together result in columns with excellent mAU
2 3 Detection: UV, 274 nm
efficiencies. These columns provide exceptional performance 4

Peaks: 1. 7-Methylxanthine
2. Theobromine
for a variety of applications in the pharmaceutical, chemical, 3. Theophylline
environmental, and food separations areas. 4. Caffeine
0
• Ultrapure silica with high-density bonding for low silanol 0 1.3 2.5 3.8 5.0 6.3 7.5 8.8 10.0
Minutes
activity
17512
• Very high efficiencies for maximum resolution
Caffeine analogs.
• Reproducible manufacturing practices for reproducible
column-to-column performance
• LC/MS compatible Wide Range of Applications
Acclaim 120 C18 columns have high surface coverage, result- The Acclaim 120 C18 can be used for the many diverse
ing in high-capacity columns. They are stable between pH 2 and reversed-phase C18 separations. Its rugged, reproducible and
8 and available in 3 and 5 µm particles sizes, and 4.6, 3.0, and reliable characteristics make it appropriate for pharmaceuti-
2.1 mm-diameters, with an average pore diameter of 120 Å. All cal, environmental, food and other industrial chromatographic
phases are LC/MS compatible because of the low silica bleed separations. The Acclaim Catalog has many examples of the use
that results from the stable bonding procedure. of this column.
LC Columns
These columns are also available for fast LC; please see the Ac- Acclaim 120 HPLC column production begins with ultrapure
claim Rapid Separation LC section. silica, which results in symmetrical peak shapes due to ultra-
low metal content. Next, a unique bonding chemistry produces
a very well-ordered and high-density silica surface coverage,
Antioxidants in Edible Oils by AOAC 983.15 on Acclaim® 120 C18 which results in highly predictable reversed-phase separations,
150 Background Information: Nine antioxidants for
unaffected by secondary retention mechanisms.
2 AOAC 983.15 spiked into wheat germ oil.
7 Extract of Spiked Column: Acclaim 120 C18, 5 µm
1 Wheat Germ Oil Dimensions: 4.6 x 150 mm
3 4 9 Mobile Phase: (A) 0.75% HOAc in H2O
5
6 8 (B) 50/50/0.5 MeOH/acetonitrile/HOAc Assay of Vanilla Extract on Acclaim® 120 C18
Inj. Volume: 10 µL
Detection: UV, 280 nm; diode array confirmation
Extract of Gradient: Time %A %B Flow
0.0 30 70 2.0 Column: Acclaim 120 C18, 3 µm, 3.0 × 75 mm
mAU Wheat Germ Oil
9.6 0 100 2.0 300 4 Pump: UltiMate LPG-3400
11.4 0 100 2.0 Mobile Phases: (A) 800 mL water + 10 mL HOAc
12.0 0 100 4.0 (B) methanol
17.0 0 100 4.0 Isocratic: 90% A, 10% B (v/v)
1 2
7 9 Flow: 0.65 mL/min
Peaks: 1. Propyl gallate
Temperature: UltiMate™ TCC-3100, 20 °C
4 2. THBP
3 5 8 Standards, 100 ng each 3. TBHQ Injection: UltiMate WPS-3000 SL, 3 µL
6 mAU 3
4. NDGA 2 Detector: UltiMate VWD-3400, 254 nm,
0 5. BHA
1 2 Hz, 0.8 s time const., micro flowcell
6. Ionox-100 Sample: Commercial vanilla extract, filtered
7. Octyl gallate Sample
2.5 Minutes 15 8. BHT
9. Dodecyl gallate Peaks: 1. p-Hydroxybenzoic acid 20 µg/mL
Note: Ethoxyquin (not shown) has been resolved under the same conditions. 20404 Standard 2. p-Hydroxybenzaldehyde 60
0
3. Vanillic acid 80
0 4 8 12 16 4. Vanillin 1200
Antioxidants in Edible Oils by AOAC Method 983.15. Minutes
Reference: AOAC Official Method 990.25
24336
Assay of vanilla extract.
120 www.dionex.com
Acclaim 120 C18
Analytical Columns
Alkaloids in Bitter Orange Extract on Acclaim 120 C18
Column: Acclaim® 120 C18, 3 µm, 3.0 × 150 mm
Acclaim 120, C18, 3 µm Analytical (2.1 × 50 mm)....................................... 059128
Pump: Summit® P680 DGP/6
Mobile Phases: Buffer: 20 mM boric acid, adj.
Acclaim 120, C18, 3 µm Analytical (2.1 × 100 mm)..................................... 059129
500 2
1 with KOH to pH 8.2
4
(A) Buffer + 10 mM Na hexanesulfonate Acclaim 120, C18, 3 µm Analytical (2.1 × 150 mm)..................................... 059130
5 (B) 20:80 acetonitrile:buffer (v/v) +
10 mM Na hexanesulfonate Acclaim 120, C18, 3 µm Analytical (3 × 150 mm)........................................ 063691
Gradient: Time -10 0 30
3
6
%A 100 100 0 Acclaim 120, C18, 3 µm Analytical (4.6 × 50 mm)....................................... 059131
mAU %B 0 0 100
Flow Rate: 0.85 mL/min Acclaim 120, C18, 3 µm Analytical (4.6 × 100 mm)..................................... 059132
Temperature: TCC-100 at 35 °C
A Inj. Volume: ASI-100 sampler at 20 µL Acclaim 120, C18, 3 µm Analytical (4.6 × 150 mm)..................................... 059133
Detector: UVD-340U; UV at 224 nm
Peaks: 1. Octopamine 4. Tyramine Acclaim 120, C18, 5 µm Analytical (2.1 × 50 mm)....................................... 059142
B 2. Synephrine 5. N-Methyltyramine
0
3. Phenylephrine 6. Hordenine Acclaim 120, C18, 5 µm Analytical (2.1 × 100 mm)..................................... 059143
Samples: (A) Standards 50 µg/mL in buffer
0 5 10 15 20 25 30
Minutes (B) Extract of bitter orange, diluted Acclaim 120, C18, 5 µm Analytical (2.1 × 150 mm)..................................... 059144
in buffer
24317 Acclaim 120, C18, 5 µm Analytical (2.1 × 250 mm)..................................... 059145
Alkaloids in bitter orange extract.
Related Literature Acclaim 120, C18, 5 µm Analytical (4.6 × 150 mm)..................................... 059148
Micro and Nano Columns
Product Brochures 75 µm i.d. × 5 cm, C18 Acclaim, 3 µm, 120Å................................................ 162238

75 µm i.d. × 15 cm, C18 Acclaim, 3 µm, 120Å.............................................. 162239
Catalog: Acclaim Bonded Silica-Based Columns for HPLC
Product Data Sheets 300 µm i.d. × 15 cm, C18 Acclaim, 3 µm, 120Å............................................ 162237
LC Columns
1.0 mm i.d. × 5 cm, C18 Acclaim, 3 µm, 120Å.............................................. 162234
Acclaim 120 HPLC Columns Data Sheet
1.0 mm i.d. × 15 cm, C18 Acclaim, 3 µm, 120Å............................................ 162235
Application Updates
75 µm i.d. × 5 cm, C18 Acclaim, 5 µm, 120Å................................................ 161456
AU 156: Evaluation of Acclaim HPLC Columns Using the Na- 75 µm i.d. × 15 cm, C18 Acclaim, 5 µm, 120Å.............................................. 161457
tional Institute of Standards Standard Reference Material 870 75 µm i.d. × 25 cm, C18 Acclaim, 5 µm, 120Å.............................................. 161458
Technical Notes 300 µm i.d. × 5 cm, C18 Acclaim, 5 µm, 120Å.............................................. 161453
300 µm i.d. × 15 cm, C18 Acclaim, 5 µm, 120Å............................................ 161454
TN 701: Sub One-Minute, Nine-Component Gradient HPLC
300 µm i.d. × 25 cm, C18 Acclaim, 5 µm, 120Å............................................ 161455
Separation for Increased Productivity Using an Acclaim 120
3 µm C18 Column 1.0 mm i.d. × 5 cm, C18 Acclaim, 5 µm, 120Å.............................................. 161450
1.0 mm i.d. × 15 cm, C18 Acclaim, 5 µm, 120Å............................................ 161451
Ordering Information 1.0 mm i.d. × 25 cm, C18 Acclaim, 5 µm, 120Å............................................ 161452
Standard particle sizes are 3 and 5 µm. Analytical columns are Micro and Nano Precolumns
available in 2.1, 3.0, and 4.6 mm diameters; standard lengths
are 50, 100, and 150 mm. Capillary formats are available in 75 300 µm i.d. × 5 mm, C18 Acclaim, 5 µm, 120Å (set of 5 cartridges)........... 162326
and 300 µm, 1.0 mm, and custom diameters, and in 50, 150, or 500 µm i.d. × 5 mm, C18 Acclaim, 5 µm, 120Å (set of 5 cartridges)........... 162324
250 mm lengths. Micro precolumn and nano precolumn car- 800 µm i.d. × 5 mm, C18 Acclaim, 5 µm, 120Å (set of 5 cartridges)........... 162323
tridges are available in several formats. Guard columns in both 1.0 mm i.d. × 5 mm, C18 Acclaim, 5 µm, 120Å (set of 5 cartridges)........... 162321
2.0 and 4.3 mm sizes packed with 5 µm particles are recom-
1.0 mm i.d. × 15 mm, C18 Acclaim, 5 µm, 120Å (set of 5 cartridges)......... 162322
mended to protect analytical columns. Columns for fast LC are
listed in the RSLC section. These columns are designed for optimal performance using
Dionex UltiMate 3000 and ICS-3000 chromatography instru-
ments.
Guard Columns
Acclaim 120, C18, 5 µm Guard Cartridges (2 × 10 mm), 2 each.................. 059447
Acclaim 120, C18, 5 µm Guard Cartridges (4.3 × 10 mm), 2 each............... 059446
Guard cartridges require guard holder.
SST Guard Cartridge Holder.......................................................................... 059456
Guard to Analytical Column Coupler............................................................ 059457
Guard Kit (Holder and Coupler)..................................................................... 059526
www.dionex.com 121
Acclaim 120 C8 Loperamide in Anti-Diarrhea Tablet on Acclaim® 120 C8
High performance reversed-phase columns for the Column: Acclaim 120 C8 3 µm, 4.6 × 150 mm
separation of small molecules 90
1
Pump:
Mobile Phases:
Summit P680A DGP
(A) Acetonitrile
(B) 3.0 g Et3N HCl + 1.0 mL H3PO4
in 550 mL water.
Acclaim 120 C8 reversed-phase columns feature a densely- Isocratic: 45% A, 55% B
Flow: 1.2 mL/min
bonded monolayer of octyldimethylsiloxane on a highly pure, mAU Temperature: TCC-100 thermostat at 30 °C
spherical silica substrate with a 120 Å pore structure. The Injection: ASI-100 sampler at 20 µL
Detector: UVD-340U, UV 214 nm
columns are a well-characterized line of LC/MS-compatible Sample Prep: – Weigh and grind 20 tablets
– Take an aliquot equivalent
C8 phases with very high surface coverage and very low metal to 16 mg loperamide HCl
0
content. These columns provide exceptional performance for – Add 40 mL of 5% H3PO4 and
200 mL of methanol
0 1 2 3 4 5 6
a variety of applications in the pharmaceutical, nutraceutical, Minutes
– Make to 2000 mL with water
– Filter
food, and life sciences.
Reference: USP, 29th Ed, p1290 (2006) Peak: 1. Loperamide HCl
23628-01
• Ultrapure silica with high-density bonding for symmetrical
peak shapes Loperamide in anti-diarrhea tablets.
• High hydrophobicity, low polarity phases for predictable
reversed-phase separations Fluticasone in Serum on Acclaim® 120 C8
15
1 2 Column: Acclaim 120 C8 3 µm, 4.6 × 150 mm
Isocratic Separation of Six Cardiac Antiarrhythmic Drugs Pump: Summit P580 HPG/4
(Beta-Blockers) on Acclaim® 120 C8 Mobile Phases: (A) Ethanol:acetonitrile 2:30
(B) Water
mAU Isocratic: 51% B
0.25 Column: Acclaim 120 C8, 3 µm Flow Rate: 1.0 mL/min
A Temperature: 30 °C
B Injection: ASI-100 autosampler; 10 µL
1 Mobile Phase: 51/49 w/w MeOH/
25 mM phosphate, pH 7.0 0 C Detector: UVD 340U; UV at 237 nm
Flow Rate: 1.0 mL/min Samples: (A) Fluticasone propionate 10 µg/mL
0 2 4 6 8 10 12 14 16 (B) Spiked bovine serum 5 µg/mL
Temperature: 40 °C Minutes
LC Columns
Inj. Volume: 5 µL (C) Blank bovine serum

AU 2
Detection: UV, 214 nm Sample Preparation: Peaks: 1. Corticosterone
6 7 – To 1.0 mL of serum add 50 µL of 2. Fluticasone propionate
3 8 Peaks: 1. Maleic acid —
5 2. Acebutolol 50 mg/mL 1 mg/mL corticosterone in ethanol
4 3. Metoprolol 50 – Extract with 1.0 mL of ethyl acetate
4. Timolol 100 – Evaporate 0.40 mL of extract
0 5. Oxprenolol 50 – Redissolve in 0.20 mL of methanol
6, 7. Labetalol 23655
0 10 diasteromers 50
Minutes 8. Propranolol 20
Fluticasone in serum.
20397
Isocratic separation of six cardiac antiarrhythmic drugs Carbamate Insecticides in EPA 531.1 on
(Beta-Blockers). Acclaim® 120 C8 with MS Detection
Background Information: Peaks: m/z
LC/MS of 12 insecticides, 1 ng each on column. 1. Aldicarb sulfoxide 207 7. Unknown 208
Overlay of 12 simultaneous single-ion chromatograms. 2. Aldicarb sulfone 240 8. Propoxur 168
Column: Acclaim 120 C8, 5 mm, 2.1 × 100 mm 3. Oxamyl 237 9. Carbofuran 222
Mobile Phase: (A) MeOH 4. Methomyl 163 10. Carbaryl 145
(B) 20 mM NH4 HCO2, pH 3.4 5. 3-Hydroxy carbofuran 220 11. Unknown 169
Gradient: Time %A %B Flow 6. Aldicarb 208 12. Methiocarb 169
0 12 88 0.25
1 12 88
15 70 30 300,000
4 9
17 70 30
18 100 0 12
8
Inj. Volume: 1–50 µL 3
Sample: 100 ppm stock diluted
Counts 1 2 5 10
1000x in Eluent B
Detection: MSQ™ MS +ESI 6
11
MS Settings: Probe 400 °C
7
Needle 2.0 kV
Cone voltage set per ion
0
4.4 17.9
Minutes
Note: MSQ is a trademark of Thermo Electron Corporation 20399
Carbamate insecticides in EPA 531.1 with MS detection.
122 www.dionex.com
Acclaim 120 C8
Related Literature
Micro and Nano Columns
For detailed specifications and applications, see the following 75 µm i.d. × 5 cm, C8 Acclaim, 3 µm, 120Å.................................................. 162208
PDF documents under Literature on www.dionex.com. 75 µm i.d. × 15 cm, C8 Acclaim, 3 µm, 120Å................................................ 162209
Product Brochures 300 µm i.d. × 5 cm, C8 Acclaim, 3 µm, 120Å................................................ 162206
Catalog: Acclaim Bonded Silica-Based Columns for HPLC 1.0 mm i.d. × 5 cm, C8 Acclaim, 3 µm, 120Å................................................ 162204
Product Data Sheets 75 µm i.d. × 5 cm, C8 Acclaim, 5 µm, 120Å.................................................. 162216
Acclaim 120 HPLC Columns Data Sheet 75 µm i.d. × 15 cm, C8 Acclaim, 5 µm, 120Å................................................ 162217
75 µm i.d. × 25 cm, C8 Acclaim, 5 µm, 120Å................................................ 162218
Application Updates 300 µm i.d. × 5 cm, C8 Acclaim, 5 µm, 120Å................................................ 162213
AU 156: Evaluation of Acclaim HPLC Columns Using the Na- 300 µm i.d. × 25 cm, C8 Acclaim, 5 µm, 120Å.............................................. 162215
tional Institute of Standards Standard Reference Material 870 1.0 mm i.d. × 5 cm, C8 Acclaim, 5 µm, 120Å................................................ 162210
Micro and Nano Precolumns
Analytical Columns
300 µm i.d. × 5 mm, C8 Acclaim, 5 µm, 120Å (set of 5 cartridges)............. 162266
Acclaim 120, C8, 3 µm Analytical (2.1 × 50 mm).......................................... 059122
500 µm i.d. × 15 mm, C8 Acclaim, 5 µm, 120Å (set of 5 cartridges)........... 162265
1.0 mm i.d. × 5 mm, C8 Acclaim, 5 µm, 120Å (set of 5 cartridges)............. 162261
LC Columns
1.0 mm i.d. × 15 mm, C8 Acclaim, 5 µm, 120Å (set of 5 cartridges)........... 162262
Guard Columns
Acclaim 120, C8, 5 µm Guard Cartridges (4.3 × 10 mm), 2 each................. 059448
Acclaim 120, C8, 5 µm Guard Cartridges (2 × 10 mm), 2 each.................... 059449
Guard cartridges require holder.
www.dionex.com 123
Acclaim PolarAdvantage Loratidine and Pseudo-ephedrine in a
Time-Release Tablet on Acclaim PA
Sulfonamide-embedded reversed-phase columns for
separating polar compounds 25
1 2
Column: Acclaim PA C16, 5 µm, 4.6 × 50 mm
Pump: Summit P680A DGP-6
Mobile Phases: (A) 15:85:0.050 acetonitrile:water:TFA
Acclaim Polar Advantage (PA) columns feature a patented m AU (B) 40:60:0.050 acetonitrile:water:TFA
Flow: 1.25 mL/min
bonding chemistry that incorporates a polar sulfonamide group 0 Temperature: 35 °C
near the surface of the silica particle. This unique chemistry 257 nm Gradient: Times 0.00 0.40 0.41 4.00
%A 100 100 0 0
–10
provides good hydrolytic stability and allows a single column to 0 1 2 3 4 %B 0 0 100 100
Minutes Injection: Summit ASI-100, 10 µL
resolve a wide variety of polar and nonpolar analytes. The Ac- Sample Preparation: Detector: Summit UVD 340U diode array,
claim PA is compatible with 100% aqueous mobile phases and – Finely grind a weighed tablet. UV at 206, 240 and 257 nm
– Weigh an aliquot containing 1 mg loratidine Peaks: 1. Pseudo-ephedrine 240 µg/mL
supports LC/MS analysis. (120 mg) into a dry 100 mL flask. 2. Loratidine 10
– Add 10 mL methanol and disperse.
– Add 3 mL of 1N HCl with mixing.
• Low silanol activity for excellent peak shape with basic – Dilute to 100 mL with water.
– Filter through 0.45 µm membrane filter. 21785
compounds
• Novel polar-embedded column chemistry Loratidine and pseudo-ephedrine in a time-release tablet.
• Compatible with 0–100% organic solvent mobile phases

• High selectivity for hydrophobic aromatic compounds Applications
• Wide range of applications The Acclaim PA column can be used in a wide range of applica-
tions in pharmaceutical, environmental, food testing and prod-
Conventional C18 phases are not compatible with highly aque- uct-quality testing. These columns also give efficient separations
ous mobile phases, due to dewetting. The Acclaim PA column with good peak shapes for compounds of life science (nucleic
by design has a mildly hydrophilic surface, which remains in acid and bases) and pharmaceutical interest (sulfonamides) us-
contact with aqueous-only mobile phases, negating the prob- ing highly aqueous mobile phases.
LC Columns
lems of dewetting common with conventional reversed-phase

columns.
LC Separation of Nitrosamines in EPA 8270 Using Acclaim® PA
Column: Acclaim PA, 5 µm
Acclaim PA columns are available both in 3 and 5 µm particle Mobile Phase:(A) D.I. H2O
1
sizes and 4.6, 3.0, and 2.1 mm diameters, with an average pore (B) Acetonitrile
Gradient: Time (min) % B
diameter of 120 Å. Acclaim PA may be used for LC/MS appli- 4 56 0 1
5 1
cations. These columns are available for fast LC; see the RSLC 7 25 90
section. AU Temperature:
30
30 °C
90
3
2 Flow Rate: 1 mL/min
89
Inj. Volume: 15 µL
Detection: UV, 230 nm
Fat-Soluble Vitamins and Carotenoids in a Vitamin Tablet Peaks (20 mg/mL): 1. N-Nitroso-dimethylamine
2. N-Nitroso-morpholine
300 1 Column: Acclaim PA C16, 3 µm, 4.6 × 150 mm 3. N-Nitroso-methylethylamine
Pump: Summit P680A DGP-6 4. N-Nitroso-pyrrolidine
6 Mobile Phase: (A) Water 5. N-Nitroso-diethylamine
4
2 3 (B) 54:44:2 methanol:acetonitrile: 6. N-Nitroso-piperidine
isopropanol 0 10 20 30 7. N-Nitroso-dipropylamine
473 nm 5 7
m AU
Gradient: Times 0 8.0 8.1 25 Minutes
8. N-Nitroso-dibutylamine
%A 5 5 0 0 Note: This gradient will also resolve benzidines. 9. N-Nitroso-diphenylamine 20109a
325 nm %B 95 95 100 100
Flow: 1.25 mL/min
0
285 nm Temperature: 30 °C LC separation of nitrosamines, as specified in EPA 8270.
Injection: Summit ASI-100, 15 µL
Detector: Summit UVD 340U diode array,
0 5 10 15 20 473, 325 and 285 nm,
Minutes and spectra 200–595 nm
Sample Preparation: Peaks: 1. trans-Retinol acetate
– Finely grind a weighed tablet. 2. trans-Lutein
– Weigh an aliquot of about 1/10 tablet (150 mg) into a screw-cap vial. 3. α-Tocopherol acetate
– Partition between 1.0 mL water and 5.0 mL ethyl acetate with 4. trans-Lycopene
vigorous shaking. 5. cis-Lycopene
– Warm to 60 °C for 10 min. 6. trans-β-Carotene
– Shake vigorously again for 0.5 min. 7. cis-β-Carotene
– Centrifuge to separate phases. 21786
Fat-soluble vitamins and carotenoids in a vitamin tablet.
124 www.dionex.com
Acclaim PolarAdvantage
Guard Columns
Phospholipids in Egg Lecithin
Acclaim PA, 5µm Guard Cartridges (2.0x10 mm), 2 each............................ 061331
Acclaim PA, 5µm Guard Cartridges (4.3x10 mm), 2 each............................ 061332
Column: Acclaim® PA C16 3µ, 4.6 × 150 mm
140
Sample: 500 µg/mL Pump: Summit P580 HPG/4
egg lecithin Mobile Phase: Acetonitrile:methanol:water:
in methanol formic acid 46.5:46.5:7.0:0.25
v/v/v/v
mV
Isocratic Flow: 1.0 mL/min
Temperature: 30 °C Guard Kit (Holder and Coupler)..................................................................... 059526
Injection: ASI-100 autosampler, 50 µL
Detector: Polymer Labs PL1000 Evaporative
0
Light Scattering Detector
0 5 10 15 20 Micro and Nano Columns
Minutes
75 µm i.d. × 5 cm, Acclaim PA, 3 µm............................................................ 162244

300 µm i.d. × 5 cm, Acclaim PA, 3 µm.......................................................... 162242
23653 300 µm i.d. × 15 cm, Acclaim PA, 3 µm........................................................ 162243
1.0 mm i.d. × 5 cm, Acclaim PA, 3 µm.......................................................... 162240
Phospholipids in egg lecithin.
1.0 mm i.d. × 15 cm, Acclaim PA, 3 µm........................................................ 162241
75 µm i.d. × 5 cm, Acclaim PA, 5 µm............................................................ 162252
Related Literature 75 µm i.d. × 15 cm, Acclaim PA, 5 µm.......................................................... 162253
300 µm i.d. × 15 cm, Acclaim PA, 5 µm........................................................ 162250
1.0 mm i.d. × 5 cm, Acclaim PA, 5 µm.......................................................... 162246
Product Brochures
Catalog: Acclaim Bonded Silica-Based Columns for HPLC 1.0 mm i.d. × 15 cm, Acclaim PA, 5 µm........................................................ 162247
LC Columns
Product Data Sheets
Acclaim PolarAdvantage HPLC Columns Data Sheet Micro and Nano Precolumns
300 µm i.d. × 5 mm, Acclaim PA, 5 µm (set of 5 cartridges)........................ 162302
Application Updates
500 µm i.d. × 5 mm, Acclaim PA, 5 µm (set of 5 cartridges)........................ 162336
AU 156: Evaluation of Acclaim HPLC Columns Using the Na- 500 µm i.d. × 15 mm, Acclaim PA, 5 µm (set of 5 cartridges)...................... 162337
tional Institute of Standards Standard Reference Material 870 800 µm i.d. × 5 mm, Acclaim PA, 5 µm (set of 5 cartridges)........................ 162335
1.0 mm i.d. × 5 mm, Acclaim PA, 5 µm (set of 5 cartridges)........................ 162333
Ordering Information 1.0 mm i.d. × 15 mm, Acclaim PA, 5 µm (set of 5 cartridges)...................... 162334
Standard particle sizes are 3 and 5 µm. Available in 2.1, 3.0,
These columns are designed for optimal performance using
and 4.6 mm i.d. Lengths are 50, 100, and 150 mm. Capillary
formats are available in 75 and 300 µm, 1.0 mm, and custom ments.
diameters, and in 50, 150, or 250 mm lengths. Micro precolumn
and nano precolumn cartridges are available in several formats.
Guard columns in both 2.0 and 4.3 mm sizes packed with 5 µm
particles are recommended. Columns for fast LC are listed in
the RSLC section.
Analytical Columns
Acclaim PA, 3µm Analytical (2.1 × 50 mm)................................................... 063174
Acclaim PA, 3µm Analytical (2.1 × 100 mm)................................................. 061316
Acclaim PA, 3µm Analytical (3 × 150 mm).................................................... 063693
Acclaim PA, 5µm Analytical (4.6 × 50 mm)................................................... 061319
www.dionex.com 125
Acclaim PolarAdvantage II Unique Selectivity
Complementary selectivity and enhanced hydrolytic
stability 4
6 Acclaim C18
Column: See chromatograms, 5 µm
3 Dimensions: 4.6 × 150 mm
5 Mobile Phase: CH3CN/25 mM potassium phosphate
Acclaim Polar Advantage II (PA2) columns feature a patented 2 buffer, pH 3.3 v/v 60/40
1 Temperature: 30 °C
bonding chemistry that provides enhanced hydrolytic stability Flow Rate: 1.0 mL/min
AU 4 Inj. Volume: 5 µL
from pH 1.5–10 and allows resolution of a wide variety of polar Acclaim PA2 Detection: UV, 206 nm
5
and nonpolar analytes. The Acclaim PA2 is compatible with 3 6 Peaks: 1. Uracil 7.5 µg/mL
2. Pyridine 50
100% aqueous mobile phases and exhibits high reversed-phase 1
2
3. Phenol 50
4. N,N-Dimethylaniline 50
capacity, with selectivity complementary to conventional C18 5. Toluene 60
columns such as the Acclaim 120 C18. As with all Acclaim 0 2 4 6 8 10 6. p-Butylbenzoic acid 50
Minutes
columns, the Acclaim PA2 is LC/MS-compatible.
20151
• Single-run analysis of polar and nonpolar analytes
• Hydrolytically stable (pH 1.5–10) The Acclaim PA2 provides selectivity that is complementary to conven-
tional C18 columns.
• Rugged column with high efficiency, symmetrical peak
shapes even with basic and acidic compounds
Omeprazole in Anti-Heartburn Tablet on Acclaim® PA2
• Compatible with 100% aqueous mobile phases Column: Acclaim PA2, 5 µm, 4.6 × 150 mm
1 Pump: UltiMate® LPG-3400
700 7
• High reversed-phase capacity with selectivity complemen- Mobile Phases: (A) Acetonitrile:methanol 85:15 (v/v)
(B) 40 mM glycine, pH 9.0
tary to conventional C18 columns Gradient Time:
%A
–7
12
0
12
20
60
mAU %B 88 88 40
mAU
Flow: 1.2 mL/min
Acclaim PA2 columns are compatible with 100% aqueous mo- Temperature: TCC-3100 thermostat at 30 °C
B Injection: WPS-3000 SL sampler at 10 µL
bile phases, overcoming many of the limitations of conventional 0 Detector: VWD-3400, UV 305 nm
A
LC Columns
0 Sample Prep: – Weigh and finely grind tablets.

C8 and C18 reversed-phase columns. Conventional reversed- – Extraction solvent: 20% ethanol,
80% (10mM borate, 1.5mM EDTA,
phase columns are unstable under these conditions and rapidly 0 5 10 15 20 pH 11.0) (v/v)
Minutes
lose their ability to retain analytes. Chromatograms
– Extract with sonication an aliquot equiv.
to 20 mg omeprazole in 100 mL solvent.
A. Omeprazole extract – Filter
Label: 20.0 mg Peak: 1. Omeprazole, approx. 225 µg/mL
Found: 19.5 mg Reference: U.S. Pharmacopeia, 29th Ed., p.1578,
Acclaim PA2 columns are available both in the 3- and 5-µm B. 100× expansion of A (2006).
particle sizes and 4.6-, 3.0, and 2.1 mm- diameters, with and 23650-01
average pore diameter of 120Å. Acclaim PA2 may be used for

Omeprazole in anti-heartburn tablet.
some LC/MS applications. These columns are available for fast
LC; refer to the RSLC section.
Highly Stable Under Wide pH Range

The proprietary bonding of the Acclaim PA2 column resists hy-
drolytic attack by protecting the bonded phase at low pH. This
column is highly stable with low pH.
In addition to low pH stability, although it is common to analyze

basic compounds under high-pH conditions to reduce peak tail-
ing, most polar-embedded phases are even less hydrolytically
stable than conventional C18 columns. The Acclaim PA2 is spe-
cifically designed to withstand high pH conditions, making it a
good choice for the separation of both basic and acidic analytes.
126 www.dionex.com
Acclaim PolarAdvantage II
Determination of Energy Drink Ingredients with Acclaim PA2 Ordering Information

Column: Acclaim PA2, 3 µm, 4.6 × 150 mm
Pump: Summit P680 DGP6 Columns for fast LC are listed in the RSLC section.
1 5 7 Mobile Phases: (A) Acetonitrile
600 3
8 (B) 30 mM phosphate buffer, pH 3.28
(C) 30 mM phosphate buffer, pH 2.54
210
nm
2 4
6 Gradient: Times 0.0 14.0 16.0 Analytical Columns
%A 0 40 40
%B 100 0 0
246 Acclaim PA2, 3 µm Analytical, (4.6 × 150 mm)............................................ 063191
mAU nm %C 0 60 60
Flow: 1.0 mL/min
265 Temperature: 30 °C Acclaim PA2, 3 µm Analytical, (4.6 × 50 mm)............................................... 063189
nm
285 Detector: UVD 340U, UV 210, 246, 265, Acclaim PA2, 3 µm Analytical, (3 × 150 mm)................................................ 063705
nm 285 nm; spectra 200–400 nm
0
Sample Prep: Filter Acclaim PA2, 3 µm Analytical, (2.1 × 150 mm)............................................ 063187
0 4 8 12 16 Peaks: 1. Ascorbic acid 5. Caffeine
Minutes 2. Pyridoxine 6. Riboflavin
Acclaim PA2, 5 µm Analytical, (4.6 × 150 mm)............................................ 063197
3. Niacinamide 7. Sorbic acid
4. Pantothenic acid 8. Benzoic acid Acclaim PA2, 5 µm Analytical, (4.6 x 250 mm)............................................. 063199
21784
Guard Columns
Determination of energy drink ingredients.
Acclaim PA2, 5 µm Guard Cartridges (2 × 10 mm), 2 each.......................... 063193
Perfluoro-octanoic Acid (PFOA) and Acclaim PA2, 5 µm Guard Cartridges (4.3 × 10 mm), 2 each....................... 063195
Perfluoro-octanesulfonic Acid (PFOS) in Tap Water Guard cartridges require holder.
1.5
A Column: Acclaim® PA2, 3 µm, 2.1 × 150 mm SST Guard Cartridge Holder.......................................................................... 059456
B 1 Pump: ICS-3000 DP
Mobile Phase: (A) Water Guard to Analytical Column Coupler............................................................ 059457
(B) 100 mM boric acid adj. to pH 8.0
34
µS
2
Flow:
(C) 70:30 acetonitrile:water
0.30 mL/min
Gradient Times: –7 0 10 15
%A 40 40 0 0
%B 30 30 30 30
0.4 %C 30 30 70 70
0 3 6 9 12 15 Temperature: ICS-3000 DC thermostat at 30 °C
Minutes Detector: ICS-3000 suppressed conductivity
LC Columns
Automated Sample Preparation Suppressor: AMMS® ULTRA-II 2mm,
Injector: ASI-100 chemical mode,
Sample Volume: 1000 µL 5 mN H2SO4 at 0.5 mL/min
Concentrator Peaks: 1. PFOA 10 ng/mL
Cartridge: Acclaim PA2 5 µm, 4.3x10mm 2. PFOS isomer 1
Wash Solution: 20 mM boric acid adj. to pH 8.0 3. PFOS isomer 2
Flow Rate: 1.0 mL/min 4. PFOS 10
Wash Time 2.5 min.
Samples: A. Sunnyvale Tap Water, spiked at 10 ng/mL
B. Sunnyvale Tap Water 23627
Perfluoro-octanoic acid (PFOA) and perfluoro-octanesulfonic (PFOS)

acid in tap water.
Related Literature
Product Brochures
Product Data Sheets
Acclaim PolarAdvantage II (PA2) HPLC Columns Data Sheet
Application Updates
AU 156: Evaluation of Acclaim HPLC Columns Using the Na-

tional Institute of Standards Standard Reference Material 870
www.dionex.com 127
Acclaim Rapid Separation LC Accelerated Assay of Vanilla Extract
Ideal for fast LC 180 LC System: UltiMate® 3000 RS
Column: Acclaim® 120 C18
Dimensions: A) 5 µm, 4.6 x 150 mm
mAU Standard
Acclaim Rapid Separation Liquid Chromatography (RSLC) A
Column
B, C) 2 µm, 2.1 x 50 mm RSLC
Mobile Phase: 200 mM HOAc in 10% (v/v) MeOH
columns use 2 and 3 µm particles for fast separations. These 0 Flow Rate: A) 1.0 mL/min
0 10 20 30 40 50 60 B) 0.41 mL/min
columns, together with the UltiMate RSLC system, provide 250 C) 0.82 mL/min
RSLC Temperature: 20 °C
ultrafast, high efficiency LC separations. mAU B Column Injection: A) 10 µL
6 x Acceleration B, C) 1.2 µL
0 Detector: UV at 254 nm,
• Accelerate separations up to fifteen-fold compared to con- 0 2.5 5 7.5 10 A) 1 Hz data rate
220 B) 5 Hz data rate
ventional LC. C) 10 Hz data rate
Double Velocity Sample: Commercial vanilla extract in 40% CH3OH, filtered
mAU C
• Accelerate sample throughput up to 30-fold compared to 12 x Acceleration Reference: AOAC Official Method 990.25
0 Peaks: 1. p-Hydroxybenzoic acid
conventional LC, with Dionex RSLC LCi Solutions. 0 1 2 3 4 5
2. p-Hydroxybenzaldehyde
3. Vanillin acid
Minutes
4. Vanillin 25460
• Save up to 85% of solvent with 3 µm columns and 92% of
solvent with 2 µm columns. Validated methods can be accelerated without changing the principle
of the analysis by using RSLC columns and simple geometric rules.
• Use as little as 15–40% of sample volume traditionally
required for LC.
70 1 4.6 × 150
5
• Achieve easy-to-use, reliable, fast separations. mA
2
3 4 6 7 8 9
0
• Ease of operation ensures optimal, fast separations 80
0 1 3 × 75 12
5
2
mA 3 4 6 7 8 9
Acclaim RSLC 2 µm columns achieve high throughput without 0
sacrificing chromatographic performance. These columns are 1.2
0 2.7
1 3 × 33
based on high-purity spherical porous silica gel. Currently, two 2 5
mA 3 4 6 7 8 9
column chemistries are available: Acclaim RSLC 2 µm C18 and
LC Columns
0
PolarAdvantage II (PA2). 0 Minutes 0.8
Systematic Acceleration of Alkyl Phenone Separation

Using RSLC with the UltiMate 3000 System
Acclaim RSLC 2 µm C18 material features high-density mono- Column
dimension
Particle
size
Flow
rate
Pressure Temp.
(bar) (°C)
Solvent
consumption
Last peak
time
Speedup
factor
(mm) (µm) (mL/min) (mL/run) (min)
meric C18 chemistry with exhaustive endcapping. As a result, it 4.6 × 150 5 1.2 119 30 17 10.8 1.0×
provides the selectivity of a standard C18 phase and extremely 3 × 75
3 × 33
3
3
1.2
2.4
167
169
50
65
4.2
2.6
2.44
0.7
4.4×
15× 23893
low silanol activity. The PA2 material features amide-embedded
chemistry with excellent hydrolytic stability (pH 1.5 to 10). In Alkyl phenone analysis shows a 15-fold increase in separation speed
addition, it provides selectivity complementary to its C18 coun- with RSLC.
terpart. Acclaim RSLC 2 µm columns are available in 2.1 mm
i.d. with 50 mm or 100 mm length. Acclaim RSLC 3 µm columns are available in three stationary
phases: Acclaim 120 C18, Polar Advantage (PA), and Polar
Advantage II (PA2). The columns are packed in 3 mm i.d. with
33 mm or 75 mm length. The inner diameter of 3 mm makes
these columns fully compatible with standard analytical LC
instrumentation, and saves up to 85% solvent compared to a
4.6 mm column of otherwise identical features.
Acclaim RSLC columns complement the Dionex UltiMate 3000

RSLC system to provide superior speed, reliability, and ease-
of-use. Using RSLC with Dionex Intelligent LC solutions for
tandem LC, parallel LC, or other techniques further increases
sample throughput up to 30-fold. See the UltiMate RSLC and
LCi Solutions sections for more information.
128 www.dionex.com
Acclaim Rapid Separation LC
Acceleration of Analysis of Pigments in Turmeric Ordering Information

with an Acclaim PA2 RSLC Column
Peaks: 1) curcumin, 2) demethoxycurcumin, 3) bisdemethoxycurcumin
A B C D E Analytical Columns
Column Size: 4.6 x 150 3.0 x 75 2.1 x 50 2.1 x 50 2.1 x 50 mm
Particle Size: 5 3 2 2 2 µm
% MeOH: 80 80 80 80 75 % Acclaim RSLC 120, C18, 3 µm Analytical (3 × 33 mm)................................. 066272
Flow Rate: 1.0 .61 .41 1.0 1.5 mL/min
400 Temperature: 30 30 30 30 60 °C Acclaim RSLC 120, C18, 3 µm Analytical (3 × 75 mm)................................. 066273
E Data Rate: 2 4 4 10 20 Hz
Injection Vol: 10 3.0 1.2 1.2 1.2 µL Acclaim RSLC PA, 3 µm Analytical (3 × 33 mm)........................................... 066274
D Speed: 1 3 4 11 22 times
Resolution: 4.4 4.2 3.3 2.6 2.0 R 1,2 Acclaim RSLC PA, 3 µm Analytical (3 × 75 mm)........................................... 066275
C Pressure: 93 144 193 462 485 bar
mAU Efficiency: 5600 4500 1600 1200 1300 Th. Plates Acclaim RSLC PA2, 3 µm Analytical (3 × 33 mm)......................................... 066276
1
B
Acclaim RSLC PA2, 3 µm Analytical (3 × 75 mm)......................................... 066277
2 3
A
0 Acclaim RSLC C18, 2 µm Analytical (2.1 x 50 mm)...................................... 068981
0 1 2 3 4 5 6 7 8 9 10 11 12 Acclaim RSLC C18, 2 µm Analytical (2.1 x 100 mm).................................... 068982
Minutes
25458 Acclaim RSLC PA2, 2 µm Analytical (2.1 x 50 mm)...................................... 068989
Acclaim RSLC columns provide different options for speed, resolution, Acclaim RSLC PA2, 2 µm Analytical (2.1 x 100 mm).................................... 068990
and pressure optimization compared to standard analytical columns.
Related Literature
Product Data Sheets
LC Columns
Acclaim Rapid Separation LC Columns Data Sheet
Technical Notes
TN 701: Sub One-Minute, Nine-Component Gradient HPLC

Separation for Increased Productivity Using an Acclaim 120
3 µm C18 Column
www.dionex.com 129
IonPac NS1 Separation of Aliphatic Sulfonic Acids Using
Polymeric reversed-phase column ideal for the Tetrabutylammonium Ion as the Ion-Pair Reagent
separation of hydrophobic, ionizable compounds 8.0 Column: IonPac® NS1 (10 mm)
Eluent: 2 mM Tetrabutylammonium hydroxide,
24% to 48% Acetonitrile in 10 min
The IonPac NS1-10µm and NS1-5µm columns are packed with Flow Rate: 1.0 mL/min
1 Inj. Volume: 50 µL
a neutral, macroporous, high-surface-area, ethylvinylbenzene 2 Detection: Suppressed conductivity,
3 ASRS®-ULTRA,
polymer cross-linked with 55% divinylbenzene. This resin µS 4 5
6 AutoSuppression® coupled with
makes the NS1 resistant to solvents, acids, and bases, and per- 7
chemical regenerant mode
Regenerant: 10 mN Sulfuric acid
mits the use of eluent from pH 0 to 14. Peaks: 1. Methanesulfonic acid 5.0 mg/L (ppm)
2. 1-Propanesulfonic acid 8.6
0.0 3. 1-Butanesulfonic acid 8.7
• Ideal for separation of large molecules that carry localized 4. 1-Hexanesulfonic acid 8.8
5. 1-Heptanesulfonic acid 8.9
charges, such as surfactants 0 2 4 6 8 10 12 14 16 6. 1-Octanesulfonic acid 8.9
Minutes 7. 1-Decanesulfonic acid 9.1
• Compatible with acids, bases, and solvent from pH 0 to 14 11140
• Can also be used for traditional polymeric reversed-phase Separation of aliphatic sulfonic acids using tetrabutylammonium ion as
applications the ion-pair reagent.
• Utilize ion-pair chromatography for difficult separations

Determination of Aliphatic Quaternary
The IonPac NS1 column is the column of choice for routine ion- Ammonium Ions Using Nonafluoropentanoate Ion
pair chromatography or ion-suppression separations using the as the Ion-Pair Reagent with an Acetonitrile Gradient
AMMS-ICE 300 suppressor. The NS1 is also used with mobile 2 Column: IonPac® NS1 (10 mm)
phase ion chromatography (MPIC®), a technique especially Eluent: 2 mM nonafluoropentanoic acid,
20% to 80% acetonitrile in 10 min
4
suited to large molecules that carry localized charges (e.g., sur- Flow Rate: 1.0 mL/min
Inj. Volume: 25 µL
factants), as well as other ions that are not amenable to separa- µS
2 5 Detection: Suppressed conductivity,
1 6 7 CSRS® ULTRA, AutoSuppression®
tion by ion-exchange chromatography. 3
LC Columns
external water mode

Peaks: (as the chloride salts)
1. Tetrapropylammonium 25 mg/L (ppm)
0 2. Tributylmethylammonium 50
3. Decyltrimethylammonium 25
4. Tetrabutylammonium 5
5. Dodecyltrimethylammonium 50
0 2 4 6 8 10 12 14 6. Tetrapentylammonium 100
Minutes 7. Hexadecyltrimethylammonium 100
11143
Determination of aliphatic quaternary ammonium ions using nonafluo-

ropentanoate ion as the ion-pair reagent with an acetonitrile gradient.
Key Applications
The IonPac NS1 column can be used for the separation of high
MW aliphatic carboxylic acids, alkyl and aromatic sulfates and
sulfonates, quaternary ammonium ions, water-soluble vitamins,
sulfur oxides, metal cyanide complexes, phenols, alkano-
lamines.
130 www.dionex.com
IonPac NS1
Determination of Aliphatic Carboxylic Acids

Using the IonPac® NS1 Column
1 Column: IonPac NS1 (10 mm) Analytical Columns

7 Flow Rate: 1.0 mL/min
Eluent 1: 24% acetonitrile, 6% MeOH,
0.03 mM HCI
IonPac NS1-10µm Analytical Column (4 × 250 mm).................................... 035321
Eluent 2: 60% acetonitrile, 24% MeOH,
0.05 mM HCI,
IonPac NS1-5µm Analytical Column (4 × 150 mm)...................................... 039568
5 mM Octanesulfonic acid
2
Gradient: 0–100% E2 in 20 min.
IonPac NS1-10µm Analytical Column (2 × 250 mm)....................................SP4354
Detection: Suppressed conductivity,
3 5 6 AMMS®-ICE Suppressor
4 8
9 11
Temp: 42 °C Guard Columns
12 Peaks: 1. Butyric 8. Dodecanoic
10 2. Pentanoic 9. Tetradecanoic
3. Hexanoic 10. Linolenic IonPac NG1-10µm Guard Column (4 × 35 mm)............................................. 039567
Baseline 4. Heptanoic 11. Linoleic
5. Octanoic 12. Hexadecanoic IonPac NG1-10µm Guard Column (2 × 50 mm).............................................SP4356
0 20 40 6. Nonanoic 13. Oleic
13 14
Minutes 7. Decanoic 14. Octadecanoic
2158-03
Determination of aliphatic carboxylic acids.
Related Literature
Product Data Sheets
IonPac NS1 Reversed Phase Polymeric Column Data Sheet
LC Columns
Application Notes
AN 139: Determination of Additives and Byproducts in an Acid

Copper Plating Bath by Liquid Chromatography
AN 145: Determination of the Suppressor Additive in Acid

Copper Plating Bath
AN 45: Fatty Acid Analysis
www.dionex.com 131
LC Columns
132 www.dionex.com
Specialty LC Columns
Columns for organic acids, surfactants, explosives
residues, and mixed-mode analysis
Dionex specialty columns include: Acclaim OA (for hydrophilic
organic acids), Surfactant (for anionic, nonionic, and cationic
surfactants), Explosives (separates all 14 explosives targeted by
EPA Method 8330), and Mixed-Mode ion-exchange columns
for separating complex mixtures. The Dionex Acclaim Mixed-
Mode WAX-1, WCX-1, and HILIC-1, and the OmniPac column
provide ion-exchange and hydrophobic interaction on a single Acclaim Organic Acid: Acclaim OA reversed-phase silica col-
column, for control when separating complex mixtures. umns are designed for separation of hydrophilic, aliphatic, and
aromatic organic acids.
• Acclaim OA for fast organic acid analysis
Acclaim Surfactant: The Acclaim Surfactant column is the most
• Acclaim Surfactant for separation of surfactants versatile commercially-available column specifically for the
separation of all classes of surfactants.
• Acclaim Explosives for separation of explosive residues
Acclaim Explosives: Acclaim Explosives columns baseline
• Acclaim Mixed-Mode for separation of complex mixtures
separate all 14 explosives in EPA Method 8330, with comple-
• Combination reversed-phase and ion-exchange specialty mentary selectivity.
phases
Acclaim Mixed-Mode HILIC-1: The Acclaim Mixed-Mode
• Unique mixed-mode selectivity complementary to RP HILIC-1 column combines both reversed-phase and hydrophilic
columns interaction liquid chromatography (HILIC) properties.
LC Columns
The novel and unique chemistries of these columns provide su- Acclaim Mixed-Mode WAX-1: Acclaim Mixed-Mode WAX col-
perior resolution and ease-of-use. Acclaim 300 C18 (page 252) umns use a silica-based stationary phase that incorporates both
and Acclaim PepMap (page 255) columns are described in the reversed-phase and weak anion-exchange properties.
Bio Columns section. Acclaim Mixed-Mode WCX-1: Reversed-phase and cation-
exchange combined on a single column.
Note: See the Acclaim Library of applications on www.dionex.
com under Literature, Application Notes. OmniPac: OmniPac polymer-based columns combine ion-
exchange and reversed-phase characteristics in a single column.
Available in both anion- and cation-exchange formats.
www.dionex.com 133
Acclaim Organic Acid Hydrophilic Aliphatic Organic Acids Using the Acclaim® OA
Optimized and application-tested for the analysis of Column: Acclaim® OA, 5 µm, 4 × 250 mm
Mobile phase: 100 mM Na2SO4, pH 2.65
hydrophilic organic acids 0.025
(adjusted with methanesulfonic acid)
The Acclaim OA column offers unparalleled separations of Inj. volume: 5 µL
hydrophilic aliphatic and aromatic organic acids using 100% 2
Peaks: 1. Oxalic acid 15 mg/L (ppm)
AU 2. Tartaric acid 120
aqueous mobile phase and at low pH, using UV detection. The 3. Formic acid 180
4. Malic acid 120
Acclaim OA column line features a patented polar-embedded 5. iso-Citric acid 120
1 34 12
stationary phase that is optimized and use-tested for hydroxy 8 6. Lactic acid 180
5 67 9 11 7. Acetic acid 120
aliphatic organic acid separations. 0
8. Citric acid 120
9. Succinic acid 120
0 5 10 15 10. Fumaric acid 7
• Use-tested to guarantee consistent hydrophilic organic acid Minutes 11. cis-Aconitic acid *
12. trans-Aconitic acid *
separations * 7 ppm total for cis and trans isomers
20011
• Compatible with 100% aqueous mobile phases, optimum
for hydrophilic organic acid retention Isocratic separation of hydrophilic organic acids.
• Hydrolytic stability at low-pH conditions, optimum for Determination of Soft Drink Ingredients with Acclaim® OA
reversed-phase retention of organic acids Column: Acclaim OA 5 µm, 4.0 × 150 mm
970 Pump: Summit P580 HPG/4
(A) Mobile Phase: (A) 14.2 g/L Na2SO4 +
• Ideal selectivity for separating a wide spectrum of organic 0.550 mL/L CH3SO3H in water
(B) Methanol
acids Gradient: Time
%A
0.0 1.0 2.0 6.5 7.5 15.0
100 100 69 69 60 60
(B) %B 0 0 31 31 40 40
Flow: 0.8 mL/min
• Excellent peak shapes for organic acids m AU Temperature: 30 °C
(C) Detector: UVD 340U, UV 210 nm;
The Acclaim OA organic acid columns can be operated at low Samples:
optional 230, 262 nm
(A) Minute-Maid® Orange Soda
4 5
(B) Diet Coke®
pH and at 100% aqueous mobile phases, conditions ideal for 1 2
3 6
7 8 (C) Mountain Dew®
LC Columns
(D) (D) Standards

retaining and separating a wide spectrum of organic acids. Ac- 0 Sample Prep.: Filter and dilute 5× with
mobile phase A
claim OA columns undergo extensive testing to ensure column- 0 5
Minutes
10 15
Peaks: 1. Erythorbic acid 50 µg/mL
2. Citric acid 500
to-column reproducibility, and are shipped with certificates of 3. Acesulfame-K 50
Note: For alternate calibration mixes, use ascorbic acid instead of erythorbic acid, 4. Aspartylphenylalanine 50
analysis detailing these tests. The Acclaim OA columns are or use saccharin instead of aspartame. 5. Caffeine 12
Minute-Maid and Diet Coke are registered trademarks of Coca-Cola. 6. Aspartame 50
packed in metal-free PEEK column bodies, to eliminate un- Mountain Dew is a registered trademark of Pepsico. 7. Sorbate potassium 50
8. Benzoic acid 50 21531
wanted interaction between the analytes and the column body.
Determination of soft drink ingredients.
Acclaim OA is the recommended column for determining small
hydrophilic organic acids, C1 to C7 aliphatic acids, and hydro-
philic aromatic acid and is also valuable for the analysis and Acclaim OA Column Applications
quality assurance of food and beverage products, pharmaceuti-
cal preparations, plating baths, and manufacturing chemicals Applications include analysis of foods, beverages, pharmaceu-
and chemical intermediates. ticals, chemical intermediates and environmental samples. For
example: aliphatic organic acids in foods (juice, wine, drinks),
organic acids in drug preparations, acrylic acid and its oligom-
ers, hydroxybenzoic acids, hydroxyphenylacetic acids, aryla-
cetic acids, benzenpolycarboxylic acids and selected amino
acids.
134 www.dionex.com
Acclaim Organic Acid
Organic Acids in Orange Juice on Acclaim® OA Ordering Information
0.07 3 Column: Acclaim OA, 5 µm Analytical Columns

2 Dimensions: 4 × 250 mm
Mobile Phase: 0.1 M Na2SO4, pH 2.68
(adjusted with methanesulfonic acid)
Acclaim OA, 5 µm Analytical (4.0 × 250 mm)............................................... 062902
Temperature: 30 °C
AU Flow Rate: 0.6 mL/min
Acclaim OA, 5 µm Analytical (4.0 × 150 mm)............................................... 062903
Inj. Volume: 5 µL
1 Sample Prep.: OnGuard II P,
diluted 2× with D.I. H2O
Guard Columns
4
0 Peaks: 1. Malic acid
2. Ascorbic acid (Vitamin C) Acclaim OA, 5 µm Guard Cartridges (4.3 × 10 mm), 2 each........................ 062925
3. Citric acid Guard cartridges require holder.
0 5 10 15 4. Fumaric acid
Minutes
20023
Analysis of organic acids in orange juice.
C1–C7 Volatile Aliphatic Acids Using the Acclaim® OA

Column: Acclaim OA, 5 µm, 4 × 150 mm
Mobile phase: (A) CH3CN
0.10 (B) 2.5 mM methanesulfonic acid
Gradient: Hold 100% B for 1 min
4,5 B to A/B (45/55) in 11 min
Hold A/B (45/55) for 4 min
Temperature: 30 °C
1 Flow rate: 1.0 mL/min
2 6 Inj. volume: 15 µL
AU 7 89 Detection: UV, 210 nm
10
3 Peaks: (10 mM each)
1. Formic acid
LC Columns
2. Acetic acid
3. Propionic acid
4. Butyric acid
0 5. Isobutyric acid
6. Isovaleric acid
0 4 8 12 16 7. n-Valeric acid
Minutes 8. Isocaproic acid
9. n-Caproic acid
10. Heptanoic acid 20117
Separation of C1 to C7 volatile aliphatic acids.
Related Literature
Product Brochures
Product Data Sheets
Acclaim Organic Acid (OA) HPLC Column Data Sheet
www.dionex.com 135
Acclaim Surfactant Complex Surfactant Separation: Inorganic Anions, Hydrotropes,
Cationic, Nonionic, Amphoteric and Anionic Surfactants
Outstanding separation of all classes of surfactants
Column: Acclaim® Surfactant, 5 µm
Mobile Phase: (A) CH3CN,
The Acclaim Surfactant column is a high-efficiency silica-based (B) 0.1 M NH4OAc, pH 5.4
column for separating a wide variety of surfactants, includ- 6 Gradient: 25% to 85% A in 25 min,
then hold 85% A for 10 min
5
ing anionic, cationic, nonionic, and amphoteric surfactants. As 10
Temperature: 30 °C
Flow Rate: 1 mL/min
a consequence of its novel chemistry, this column exhibits a mV
3
Inj. Volume:
Detection:
25 µL
ELS detector
unique polarity that provides significantly improved resolution 2 4 Peaks: 1. Chloride 11. C10-LAS
7 13 2. Bromide 12. C11-LAS
for individual oligomers of ethoxylated surfactants compared 8 9
1 12 3. Nitrate 13. C12-LAS
4. Xylene sulfonate 14. C13-LAS
with conventional C18 columns. 11 14 5. Laurylpyridinium chloride
6. Lauryldimethylbenzyl-
0 5 10 15 20 25 30 35 ammonium chloride
• Ideal selectivity for mixtures of different types of surfac- Minutes 7. Triton X-100
8. Cetyl betaine
tants 9. Decyl sulfate
10. Dodecyl sulfate 22793
• Excellent peak shapes for cationic surfactants
Inorganic anion, hydrotropes, cationic, nonionic, amphoteric, and
• Compatible with highly aqueous mobile phases
anionic surfactants.
• Improved resolution for ethoxylated surfactants
• Rugged separations under a variety of conditions Analysis of ZONYL FSO Fluorosurfactant
Column: Acclaim Surfactant, 5 µm

• Methods compatible with various detectors Dimensions: 4.6 × 150 mm
Mobile Phase: (A) Acetonitrile, (B) H2O
Gradient: 40–70% A in 30 min, then
The Acclaim Surfactant is also resistant to dewetting under hold at 70% A for 5 min
highly aqueous mobile phase conditions, and thus can be used Flow Rate: 1 mL/min
Inj. Volume: 10 µL
to provide excellent resolution between strongly hydrophilic Detection: ELSD RfCH2CH2O(CH2CH2O)xH
mV
compounds, such as isomers of xylene sulfonate. Rf = F(CF2CF2)y
LC Columns
x = 0 to about 15
y = 1 to about 7
Zonyl FSO fluorosurfactant
0 10 20 30 35
Minutes
21081
Analysis of ZONYL FSO fluorosurfactant.
Broad Range of Applications

Surfactants are widely used in industrial, agricultural, and
pharmaceutical markets, in products as diverse as pesticides,
detergents powders, petroleum products, cosmetics, and phar-
maceuticals. The Acclaim Surfactant column has been designed
specifically for the HPLC separation of these surfactants.
The Acclaim Surfactant column can be used for the HPLC

separation of anionic surfactants (alkylbenzenesulfonates, alkyl
sulfates, alkylether sulfates) and cationic surfactants (alkyl
quaternary ammonium salts, benzylalkylammonium salts, pyri-
dinium salts, and quaternary imidazolium compounds) nonionic
surfactants, and polyethylene glycols (PEGs).
136 www.dionex.com
Acclaim Surfactant
Separation of Different Polyethylene Glycols
Analytical Columns
Column: Acclaim® Surfactant, 5 µm
Mobile Phase: (A) Acetonitrile, (B) H2O Acclaim Surfactant, 5 µm Analytical (4.6 × 150 mm)................................... 063201
Gradient: 3–15% A in 20 min
Temperature: 30 °C Acclaim Surfactant, 5 µm Analytical (2.1 × 150 mm)................................... 068123
PEG-300 Flow Rate: 1 mL/min
Inj. Volume: 10 µL Acclaim Surfactant, 5 µm Analytical (4.6 × 250 mm)................................... 063203
mV
Detection: ELSD
PEG-400
PEG-600
Guard Columns
Acclaim Surfactant, 5 µm, Guards (4.3 × 10 mm), 2 each........................... 063215
0 5 10 15 20
Minutes Acclaim Surfactant, 5 µm, Guards (2.1 × 10 mm), 2 each........................... 068124
21077 Guard cartridges require holder.
Separation of different polyethylene glycols. SST Guard Cartridge Holder.......................................................................... 059456

Separation of Cationic Surfactants with ELS Detection Guard Kit (Holder and Coupler)..................................................................... 059526
Column: Acclaim Surfactant, 5 µm
Mobile Phase: (A) Acetonitrile
(B) 1% acetic acid
3 5 Gradient: Time (min) A B
–12 20 80
2 0 20 80
25 80 20
Temperature: 30 °C
mV 10 Inj. Volume: 10 µL
6 Detection: ELS detector (gain 8,
4 9 evaporating temperature 50 ºC)
Peaks: (100–400 µg/mL each)
7 1. Lauryl pyridinium
LC Columns
2. Lauryldimethylbenzyl ammonium
3. Octylphenoxyethoxyethyl-
dimethylbenzyl ammonium
8 4. Cetyltrimethylammonium
5. Cetylpyridinium
6. Diethyl heptadecyl imizolinium – 1
7. Diethyl heptadecyl imizolinium – 2
0 5 10 15 20 25 8. 2M2HT quart – 1
Minutes 9. 2M2HT quart – 2
10. 2M2HT quart – 3 23803
Separation of cationic surfactants.
Related Literature
Product Brochures
Product Data Sheets
Acclaim Surfactant Column Data Sheet
www.dionex.com 137
Acclaim Explosives Comparison of Elution Patterns of Acclaim® Explosives E1 and E2
Acclaim Explosives columns: a total solution for EPA
Method 8330 3
4 Peaks:
1. HMX
1 Acclaim Explosives E1 2. RDX
2 5 7 11 3. 1,3,5-Trinitrobenzene
The Acclaim Explosives E1 and E2 columns are 5 µm silica- 6 8 9 10
12 13 14 4. 1,3-Dinitrobenzene
based reversed-phase columns designed to resolve all 14 5. Nitrobenzene
6. Tetryl
explosives listed in EPA SW-846 Method 8330: Nitroaromatics AU
4 7. 2,4,6-Trinitrotoluene
3 8. 4-Amino-2,6-Dinitrotoluene
and Nitramines by HPLC. Their novel and unique chemistries Acclaim Explosives E2 9. 2-Amino-4,6-Dinitrotoluene
5 11 0. 2,6-Dinitrotoluene
deliver superior resolution with complementary selectivities. 7
11. 2,4-Dinitrotoluene
2
The E1 is recommended for use as a direct replacement for 1 610 12. 2-Nitrotoluene
12 13 14 8 9
13. 4-Nitrotoluene
ODS columns for the primary analysis. The E2 may be used as 14. 3-Nitrotoluene
either a primary or a confirmatory column. 0 9 18 27 36 45

Minutes
22787
• Two columns, each offering baseline resolution of all 14
compounds targeted by EPA Method 8330 Complimentary baseline separation of 14 target compounds listed in
EPA SW-846 Method 8330.
• Two columns with mutually-complementary selectivity
• Simple isocratic elution conditions Gun Surveillance Standards on Acclaim® Explosives E2
• Rugged columns with good lot-to-lot reproducibility Column: Acclaim Explosives E2,
5 µm 4.6 × 250 mm
Pump: Summit P680A DGP
1
• Unique selectivities for separating other nitro-aromatic 15 4
Mobile Phase: 37% 2mM ammonium acetate pH 5
58% methanol
molecules 5% acetonitrile (v/v/v)
Flow: 1.25 mL/min
Injection: ASI-100 sampler at 30 µL
The Acclaim Explosives stationary phases are based on 5 µm 3 5 Detector: UVD 340U at 254 nm,
mAU 2 and spectra 200–595 nm
ultra-high purity spherical silica gel particles, with 120Å pore 9
Note: Resolution may be fine-tuned
67
LC Columns
by temperature adjustments
size. The unique chemistry of these columns offers exceptional 8 Peaks: 1. Dimethylphthalate 20 µg/mL
selectivity and stability over the range of pH 3.0 to 7.0, and their 2. N-Nitrosodiphenylamine 5
3. 2,2'-Dinitrodiphenylamine 5
0
design ensures good column life and easy cleanup. 4. Diphenylamine 20
5. 2,4'-Dinitrodiphenylamine 5
0 10 20 30 6. 2,4-Dinitrodiphenylamine 5
Minutes 7. 4-Nitrodiphenylamine 5
While both columns perform reproducibly under their optimized 8. 4,4'-Dinitrodiphenylamine 5
9. 2-Nitrodiphenylamine 5
conditions, separation conditions for the Acclaim Explosives E2 Standards in 50% acetonitrile 24330
can be readily changed to accommodate inclusion of additional

Gun surveilance standards on Acclaim Explosives E2 column.
explosives compounds.
The manufacturing process is controlled to ensure that every
Explosives column delivers reproducible performance, lot-to-
lot and column-to-column. The columns are tested for baseline
resolution of all 14 explosives.
138 www.dionex.com
Acclaim Explosives
ISO22478 Explosives on Acclaim® Explosives E2 Ordering Information

Column:
Acclaim Explosives E2,
700 5 µm 4.6 × 250 mm
20 Pump: UltiMate LPG3400 Analytical Columns
Mobile Phases: (A) Acetonitrile
(B) Methanol
5 (C) 2mM ammonium acetate pH 5 Acclaim Explosives E1 (4.6 × 250 mm)......................................................... 064305
Gradient: Time –8 0 12 27 37 45
(A) 2 2 0 0 0 0
1 11 12 (B) 44 44 46 51 80 80 Acclaim Explosives E2 (4.6 × 250 mm)......................................................... 064309
13 17 18 (C) 54 54 54 49 20 20
mAU 4
78 9
14 16
Flow: 1.25 mL/min Acclaim Explosives Kit.................................................................................. 064312
15 Injection: WPS-3000 SL sampler at 50 µL
Detector: VWD-3400 at 210 and 254 nm
23 Peaks: 1. HMX 12. 2,6-DNT
A 21
2. EGDN 13. 2,4-DNT Guard Columns
19 3. DEGDN 14. 2-NT
B 6 10
4. RDX 15. 4-NT
0 5. 1,3,5-TNB 16. 3-NT Acclaim Explosive E1 Guard (4.3 × 10 mm), 2 each..................................... 064303
6. Picric acid 17. 4-A-2,6-DNT
7. 1,3-DNB
8. NB
18. 2-A-4,6-DNT
19. PETN
Acclaim Explosive E2 Guard (4.3 × 10 mm), 2 each..................................... 064307
0 10 20 30 40 45
Minutes
9. 2,4,6-TNT 20. DPA
10. Nitroglycerin 21. HNDPA
11. Tetryl 24332
ISO22478 explosives on Acclaim Explosives E2 column. Guard to Analytical Column Coupler............................................................ 059457
Column: Acclaim Explosives E2, Temperature: TCC-3200 at 28 °C

5 µm 4.6 × 250 mm Injection: WPS-3000 SL sampler at 10 µL
Pump: UltiMate™ 3000 LPG-3400 Detector: VWD-3400 UV at 360 nm
Mobile Phases: (A) acetonitrile
(B) 2 mM ammonium acetate pH 5 Gradient:
Flow: 1.5 mL/min Time –9 0 20 28 32
%B 48 48 48 10 10
55
1
Peaks:
1. Formaldehyde-DNPH
2. Acetaldehyde-DNPH
3. Acetone-DNPH
13 4. Acrolein-DNPH
mAU 2 11 5. Propionaldehyde-DNPH
6. Crotonaldehyde-DNPH
34 10 12
LC Columns
5 89 7. Butanone-DNPH
6 7 8. Methacrolein-DNPH
9. Butyraldehyde-DNPH
10. Benzaldehyde-DNPH
0 11. Valeraldehyde-DNPH
12. m-Tolualdehyde-DNPH
13. Hexaldehyde-DNPH
0 5 10 15 20 25 30
Minutes 24090
CARB1004 carboxyl-DNPH stardards.
Related Literature
Product Brochures
Product Data Sheets
Acclaim Explosives Columns A Total Solution for Explosives

Analysis
www.dionex.com 139
Acclaim Mixed-Mode HILIC-1 Reversed-Phase and HILIC Capabilities with One Column
Reversed-phase and hydrophilic interaction phases
combined on a single column Reversed-Phase Mode
1
Column: Acclaim Mixed-Mode HILIC-1, 5 µm
The Acclaim Mixed-Mode HILIC-1 column utilizes a unique 2
Dimensions:
Mobile Phase:
4.6 × 150 mm
CH3CN/0.1 M NH4OAc, pH 5.2
silica-based, mixed-mode stationary phase that combines both v/v 52/48 for RP mode
v/v 92/8 for HILIC mode
reversed-phase (RP) and hydrophilic interaction liquid chroma- AU Temperature: 30 °C
tography (HILIC) properties. The column functional group fea- HILIC Mode Inj. Volume: 10 µL
1
tures a hydrophobic alkyl chain and hydrophilic diol functional- Detection:
Peaks:
UV at 254 nm
1. Cytosine (100 ppm)
ity. This combination allows both hydrophobic interaction and 2. Naphthalene (100 ppm)
hydrophilic interaction to be utilized to optimize separations.

0 2 4 6 8 10
Minutes
• Operates in both RP and HILIC modes
24254
• Retains highly polar molecules

All the advantages of reversed-phase and HILIC compatibilities in one
• Unique selectivity complementary to RP columns column.
• Superior performance compared to conventional diol-based

columns Analysis of an Alkylphenol Ethoxylate
Using RP and HILIC Modes
• High efficiency column for high resolution separations A/B = 40/60 Column: Acclaim Mix-Mode HILIC-1, 5 µm
A/B = 52/48 Mobile Phase: A –CH3CN
The Acclaim Mixed-Mode HILIC-1 columns consist of 5 µm B –0.1 M NH4OAc, pH5.2
A/B = 55/45
high-purity, porous, spherical silica with 120 Å-diameter pores
RP
Temperature: 30 °C
A/B = 62/38 Flow Rate: 1.0 mL/min
bonded with proprietry functional groups, which provide unsur- Inj. Volume: 10 µL
AU A/B = 75/25 Detection: UV @ 225 nm
passed resolution and peak symmetry for a variety of polar and Sample: IGEPAL CA-630 (0.1%)
LC Columns
A/B = 87.5/12.5
nonpolar molecules.
A/B = 95/5
A/B = 97.5/2.5 C8 H17 (OCH2 CH2)n OH
NP
n~9
A/B = 99/1
IGEPAL CA-630
0 6 12 18 24 30
Minutes 24256
Note the advantages for reversed-phase and normal-phase modes on a

single column.
Wide Range of Applications

The Acclaim Mixed-Mode HILIC-1 column separates both
polar and non-polar molecules with selectivity complementary
to RP columns and is suitable for a broad range of applications,
including drug metabolites, lipids, polyethylene glycols (PEGs),
ethoxylated surfactants and more.
140 www.dionex.com
Acclaim Mixed-Mode HILIC-1
Separation of Phospholipids
Analytical Columns
Mobile Phase: 45/5/50 v/v/v Acclaim Mixed-Mode HILIC-1, 5 µm (4.6 × 150 mm)................................... 066843
CH3CN/MeOH/0.1 M NH4OAc, pH 5.2
Temperature: 30 °C Acclaim Mixed-Mode HILIC-1, 5 µm (4.6 × 250 mm)................................... 066844
AU Flow Rate: 1 mL/min
Inj. Volume: 20 µL Acclaim Mixed-Mode HILIC-1, 5 µm (2.1 × 150 mm)................................... 066847
Detection: ELS detector
Sample: Lecithin (chicken egg yolk) 2 mg/mL
Guard Columns
Acclaim Mixed-Mode HILIC-1 Guard, 5 µm (4.3 × 10 mm), 2 each............. 066845
Acclaim Mixed-Mode HILIC-1 Guard, 5 µm (2.1 × 10 mm), 2 each............. 066846
0 4 8 12 16 20
Minutes 24316
Separation of phospholipids (lecithin from chicken egg yolk). Guard to Analytical Column Coupler............................................................ 059457
Analysis of Polyethylene Glycols

PEG-6000 Dimensions: 4.6 × 150 mm
Mobile Phase: (A) CH3OH
(B) D.I. H2O
Gradient: 20% to 95% A in 20 min
Temperature: 30 °C
mV PEG-3400 Flow Rate: 1 mL/min
Inj. Volume: 25 µL
PEG-2000 Detection: ELS detector
Samples: Various PEGs (0.04% each)
LC Columns
PEG-400
PEG-1000
0 4 8 12 16 20
Minutes
24260
Analysis of polyethylene glycols.
Related Literature
Product Data Sheets
Acclaim Mixed-Mode HILIC-1 Column Datasheet
www.dionex.com 141
Acclaim Mixed-Mode WAX-1 Gradient Separation of Basic, Neutral, and Acidic Pharmaceuticals
Reversed-phase and weak anion-exchange properties Column: Acclaim Mixed-Mode WAX-1, 5 µm
Dimension: 4.6 × 150 mm
on a single column 4 13 Mobile Phase: (A) Acetonitrile;
(B) D.I. water;
(C) 150 mM phosphate buffer, pH 6.0
2 Gradient: Time %A %B %C
The novel column chemistry of the Acclaim Mixed-Mode 11 -15 10 80 10
AU 9 12 0 10 80 10
WAX-1 results in a multimode separation mechanism including 1
10 50 40 10
3 5 78 12 50 40 10
hydrophobic, anion-exchange, and cation-exclusion interactions. 6 10
14 25 50 0 50
15 16
Consequently, retention of basic, neutral, and acidic molecules 40
Temperature: 30 °C
50 0 50
can be either independently or concurrently adjusted by chang- 0 20 40

Flow Rate: 1 mL/min
Inj. Volume: 15 µL
ing ionic strength, pH, and organic solvent content in the mobile Minutes Detection: UV, 220 nm
Peaks: µg/mL
phase. 1. Caffeine 50 6. Hydrocortisone 100 11. Salicylic acid 50
2. Acetaminophen 100 7. Dexamethasone 100 12. Ketoprofen 100
3. Dextromethorphan 100 8. Acetyl salicylic acid 50 13. Naproxen, Na salt 100
4. Amitriptyline 100 9. Sorbate, potassium 100 14. Aconitic acid, cis- 50
• Adjustable selectivity 5. Prednisone 100 10. Benzoic acid 100 15. Aconitic acid, trans- 50
16. Ibuprofen 100 23716
• Selectivity orthogonal to reversed-phase (RP) columns

Separation of acids, bases, and neutral molecules in a single run.
• Ideal selectivity for anionic molecules
• Simultaneous separation of acidic, basic, and neutral mol-
ecules
• Multimode retention mechanisms: reversed- and normal
phase, anion-exchange, cation-exclusion, and HILIC modes
The Acclaim Mixed-Mode WAX-1 column combines hydropho-

bic and anion-exchange characteristics, on a 5 µm high-purity,
porous silica with 120-Å diameter pores. Because of this unique
LC Columns
chemistry, the Acclaim Mixed-Mode WAX-1 column also pro-

vides complimentary selectivity to C18 reversed-phase columns.
Hydrophilic organic acids are often difficult to separate with

common RP columns, since hydrophobic retention alone is
inadequate to differentiate molecules with similar hydropho-
bicities. This can be overcome using the Acclaim Mixed-Mode Separation of monocarboxylic acids.
WAX-1 column; it provides sufficient retention, and ideal
selectivity for a variety of anionic molecules, even for weakly
charged molecules. Multi-Mode Separation Mechanism
Retention of basic, neutral and acidic molecules can be either
independently or concurrently
Separation adjusted by changing
of Monocarboxylic Acids ionic
strength, pH, and organic solvents content in the mobile phase.
The presence of2anion-exchangeColumn:
functionalityAcclaim control through
Mixed-Mode WAX-1, 5 µm
electrostatic attraction (anions), repulsion (cations).
Mobile Phase: 25 mM phosphate The alkyl
buffer, pH 6
Temperature: 30 °C
chain controls separation by hydrophobic
Flow Rate: interaction.
0.8 mL/min
Inj. Volume: 10 µL
AU
A broad range of 3samples in the Peaks:
pharmaceutical,
1. Quinic acidfood and
6
2. Shikimic acid
beverage and chemical
1 4
5
industries
8 can be separated.
3. Glycolic acid
Separation
7
of basic active ingredients and9its counter-ion
4. Lacticcan
acid be simultane-
5. Acetic acid
ously determined, as well as separation of 6.ingredients
Formic acid contain-
ing basic, neutral, and acidic ingredients. 7.8. Ascorbic acid (Vitamin C)
Iso-ascorbic acid
0 5 10 15 9. Propionic acid
Minutes
23712
142 www.dionex.com
Acclaim Mixed-Mode WAX-1
Analysis of a Pain Relief Medicine Ordering Information

Column: Acclaim Mixed-Mode WAX-1, 5 µm
HN
O
O NH2 Mobile Phase: 40/60 v/v Acetonitrile/buffer Analytical Columns
OH (6.8 g potassium monophosphate and
3 0.5 g pyrophosphate in 1000 g D.I. H2O,
OH pH is adjusted to 6.0 with NaOH) Acclaim Mixed-Mode WAX-1, 5 µm (4.6 × 250 mm)................................... 064985
Temperature: 30 °C
O
N O OH Flow Rate: 1 mL/min Acclaim Mixed-Mode WAX-1, 5 µm (4.6 × 150 mm)................................... 064984
N
Inj. Volume: 1 µL
O N N 2 O O
Detection: UV, 220 nm Acclaim Mixed-Mode WAX-1, 5 µm (2.1 × 150 mm)................................... 067084
AU 1 Me Peaks:
4 1. Caffeine 4. Acetyl salicylic acid (Aspirin)
2. Acetaminophen 5. Salicylic acid
O OH
OH
3. Salicylamide Guard Columns
Sample Preparation:
1. Grind 500 mg tablet into fine powder.
5
2. Weigh 400 mg of the powder and dissolve
Acclaim Mixed-Mode WAX-1, 5 µm Guard Cartridges (4.3 × 10 mm),
with 40 g of D.I. water. 2 each............................................................................................................. 064986
0 10 20 3. After sonicating for 5 min, the filter suspension
Minutes with 0.45 µm membrane filter.
4. Dilute the filtrate fourfold with D.I. water
Acclaim Mixed-Mode WAX-1, 5 µm Guard Cartridges (2.1 × 10 mm),
23719
2 each............................................................................................................. 067085
Analysis of a pain relief medicine. SST Guard Cartridge Holder.......................................................................... 059456
Analysis of Soft Drinks
1 Column: Acclaim Mixed-Mode WAX-1, 5 µm
A (pH 6.0)
4 Dimension: 4.6 × 150 mm
2 Mobile Phase: (A) 55/45 v/v Acetonitrile/
3 5
6 7 0.2 M phosphate buffer, pH6.0
AU 1 (B) 57/43 v/v Acetonitrile/
0.12 M phosphate buffer,
B (pH 2.9)
2 4 pH2.9
Temperature: 30 °C
5 6 7 3
Flow Rate: 1 mL/min
Inj. Volume: 2.5 µL
0 5 10 Detection: UV, 210 nm
Minutes Peaks: 1. Caffeine 100 µg/mL
2. Aspartame 100
LC Columns
O
O O O O 3. Acesulfame, 100
N N S
H3N+ N
O
O N– K+ NH potassium
N O
O N O– H S O 4. Saccharin 100
O O
O 5. Sorbate, potassium 100
1. Caffeine 2. Aspartame 3. Acesulfame, potassium 4. Saccharin
O 6. Benzoic acid 100
O OH
O
O OH
OH
7. Citric acid 300
O– K+ HO O
HO
5. Sorbate, potassium 6. Benzoic acid 7. Citric acid 23722
Analysis of soft drinks.
Related Literature
Product Data Sheets
Acclaim Mixed-Mode WAX-1 Column: Total Control

of Selectivity
www.dionex.com 143
Acclaim Mixed-Mode WCX-1 WCX vs. WAX – Orthogonal Selectivity
Reversed-phase and weak cation-exchange on a
single column Mixed-Mode WCX-1
Column: 5 µm
2 Dimension: 4.6 × 150 mm
Mobile Phase: 60/40 v/v MeCN/
The Acclaim Mixed-Mode WCX-1 is a novel, high-efficiency, 1
20 mM sodium phosphate buffer,
pH 6.5
silica-based column, manufactured by bonding a specially 3 Temperature: 30 °C
designed proprietary ligand with both hydrophobic and weak AU
Flow Rate: 1 mL/min
Inj. Volume: 5 µL
cation-exchange properties. Selectivity of ionizable and neutral 2 Mixed-Mode WAX-1 Detection: UV (215 nm)
Peaks: 1. Benzoic acid 200 µg/mL
compounds can be controlled independently or simultaneously 2. Naphthalene 50
by tuning mobile phase ionic strength, pH or organic modifier. 3
1
3. Benzyl amine 300
This column therefore can separate using multiple separation

modes: reversed phase, cation exchange, and normal phase/ 0 4 8
HILIC. Minutes
24912
• Adjustable selectivity Orthogonol selectivity.

• Ideal selectivity for separating basic molecules
• Selectivity complemenatry to RP columns Simultaneous Separation of Acidic, Neutral
and Basic Pharmaceuticals
• Multimode separation mechanism: RP, Cation-exchange,
Column: Acclaim Mixed-Mode WCX-1, 5 µm
Ion-exclusion and HILIC Dimension: 4.6 × 150 mm
1
Mobile Phase: 40/60 v/v MeCN/NH4OAc,
• Capable of separating a mixture of neutral and basic com- 3
pH 5.2 (20 mM total)
Temperature: 30 °C
pounds 7
Flow Rate: 1 mL/min
AU Inj. Volume: 5 µL
Detection: UV (225 nm)
The adjustable selectivity of the Mixed-Mode WCX-1 makes 2
5
Peaks: 1. Maleate 50 µg/mL
LC Columns
2. Ketoprofen 30
it an ideal column for secondary methods development since 3. Naproxen 30
4 6
the selectivity is orthogonal to C18 columns and weak anion- 4. Hydrocortisone 60
5. Dexamethasone 60
exchange (WAX) columns. 6. Oxprenolol 300
7. Timolol 250
0 4 8
Minutes
The Acclaim Mixed-Mode WCX-1 columns novel column 24914
chemistry offers multiple retention mechanisms, including

RPLC, cation-exchange, ion-exclusion and HILIC. This feature Simultaneous separation of acidic, neutral and basic pharmaceuticals.
makes this column versatile in many applications that require
different selectivities.
Broad Range of Applications
Basic compounds are important in a variety of industrial appli-
cations, including pharmaceutical, chemical, consumer prod-
ucts, food & beverages, and more. However, analyses of these
compounds are often challenging when using reversed-phase
silica columns. The Acclaim Mixed-Mode WCX-1 provides
a remedy to these difficulties: not only does it retain basic
molecules (from highly hydrophilic to highly hydrophobic), but
also separates them with symmetrical peak shapes and excellent
efficiency.
The Acclaim Mixed-Mode WCX-1 column can serve as a pri-

mary column for a variety of basic analytes containing samples
as well as a secondary column that provides selectivity orthogo-
nal to that of reversed-phase columns. Thus it is an invaluable
tool for HPLC method development for a broad range of ap-
plications.
144 www.dionex.com
Acclaim Mixed-Mode WCX-1
Separation of Antidepressants

Analytical Columns
Mobile Phase: 50/50 v/v MeCN/10 mM NH4OAc, pH 5.2 Acclaim Mixed-Mode WCX-1, 5 µm (4.6 × 250 mm)................................... 068352
Temperature: 30 °C
Flow Rate: 1 mL/min Acclaim Mixed-Mode WCX-1, 5 µm (4.6 × 150 mm)................................... 068353
Inj. Volume: 5 µL
Detection: UV (215 nm)
AU
Peaks: 100 ppm each
1
4 5 6 1. Doxepin (mixture of isomers)
Guard Column
2 2. Imipramine
3 3. Trimipramine Acclaim Mixed-Mode WCX-1 Guard, 5 µm (4.3 × 10 mm), 2 each............. 068354
4. Amitriptyline
(As. = 1.08, 11623 plates/column)
5. Protriptyline
6. Nortriptyline
0 10
Minutes
20 Guard Kit (Holder and Coupler)..................................................................... 059526
24913 Guard Kit (Holder and Coupler)..................................................................... 059526
Separation of antidepressants.
Simultaneous Determination
of an Acidic Drug and its Counterion

1 Mobile Phase: 30/70 v/v MeCN/NH4OAc,
pH 5.2, 5 mM (total)
Temperature: 30 °C
5 mM
Inj. Volume: 2 µL
mV
1 Detection: ELS detector
Sample: Naproxen sodium salt (1 mg/mL)
LC Columns
Peaks: 1. Naproxen
2 2. Na+
10 mM
0 4 8
Minutes
24993
Analysis of naproxen sodium salt.
Related Literature
Product Datasheets
Acclaim Mixed-Mode WCX-1 for Separating Basic Molecules

Data Sheet
www.dionex.com 145
OmniPac Gradient Separation of Inositol Mono-, Di-, Tri-, and
DVB polymer columns for combined ion-exchange Tetraphosphates in One Injection
and reversed-phase separations Column: OmniPac® PAX-100 (4 x 250 mm)

Eluent: Isopropanol / Sodium Hydroxide
Gradient
The OmniPac PAX-100 column is used to separate hydropho- Flow Rate: 1.0 mL/min
Detection: Suppressed Conductivity
bic anionic analytes such as larger organic acids. The OmniPac 8
9 (Baseline Subtracted)
PAX-500 simultaneously separates anionic and neutral spe- 2
5
6
7
10 Peaks: 1. Ins (2) P
1 2. Ins (1) P
cies. The OmniPac PCX-100 separates low-molecular-weight 3 4 3. Ins (4) P
4. Ins (1,4) P2
hydrophobic cations. The OmniPac PCX-500 simultaneously 5. Ins (2,4) P2
6. Ins (4,5) P2
separates cationic and neutral species in a single run. 7. Ins (1,3,4) P3
8. Ins (1,4,5) P3
0 10 20 30 40 50 60 9. Ins (2,4,5) P3
• Acid-, base-, and solvent-compatible, pH 0–14 Minutes 10. Ins (1,4,5,6) P4
• Ideal for the separation of high-molecular-weight organic 5678
acids
Gradient separation of inositol mono-, di-, tri-, and tetraphosphates.
• Delivers optimal separation of very hydrophobic anions
• Delivers optimal separation of halogenated anions Isocratic Separation of Aromatic Amines
• Delivers simultaneous separation of neutral and ionic spe- 2

3
cies Column: OmniPac® PCX-100 (4 x 250 mm)
Eluent: 160 mM Cesium Chloride /
80 mM Hydrochloric Acid /
• Unique selectivity for polar and ionic organic analytes 4 5 20% Acetonitrile, Isocratic
1
Flow Rate: 1.0 mL/min
6
• Delivers optimal separation of organic, hydrophobic, and Detection: UV, 215 nm
Peaks: 1. Methylpseudoephedrine
halogenated cations 2. Pseudoephedrine
3. Norpseudoephedrine
4. Dimethylphenethylamine
LC Columns
The OmniPac PAX- and PCX-100 and 500 are latex-based 5. Methylphenethylamine
6. Phenethylamine
columns. Both PAX columns have an ion-exchange capacity of
about 40 μeq per column, providing equivalent anion-exchange
separations. The PCX columns have a capacity of approxi- 4
0 8
mately 120 μeq per column. The PAX- and PCX-500 columns Minutes 5698
separate analytes through both ion-exchange and reversed-phase

mechanisms, due to their higher reversed-phase capacity relative Isocratic separation of aromatic amines.
to the PAX- and PCX-100.
The OmniPac PAX-100 column was one of the first 100% Wide Range of Applications
solvent-compatible, anion-exchange columns developed for
Use the OmniPac PAX-100 column for analysis of larger or-
the separation of inorganic and organic anions, providing acid
ganic acids, including alkylbenzene sulfonates, aromatic acids,
and base compatibility over the entire pH range. Because the
polyphosphates, and inositol phosphates. Use the OmniPac
OmniPac PAX-100 is solvent compatible, solvents can be used
PAX-500 for separation of alcohols, alkanolamines, antihis-
to modify the ion-exchange selectivity.
tamines, anti-inflammatory agents, aromatic acids, inorganic
acids, peptides, purines and pyrimidines, and sulfonamides.
The OmniPac PCX-100 column can be used to separate hydro-

phobic cationic analytes such as amines, anilines, antidepres-
sants, drugs, and metabolites. The OmniPac PCX-500 may
be used for the separation of adrenergics, alcohols, anilines,
antidepressants, anti-inflammatory agents, plating bath brighten-
ers, cephalosporins, stains and dyes, herbicides, nucleotides, and
nucleosides.
146 www.dionex.com
OmniPac
Gradient Separation of N-Containing Compounds

Using the OmniPac® PCX-500 Column
Analytical Columns
16
12 Column: OmniPac PCX-500 (4 × 250 mm)
13 Eluent: Acetonitrile / Sodium Chloride / OmniPac PAX-100 Analytical Column (4 × 250 mm).................................... 042150
9 Hydrochloric Acid Gradient
6,7 Flow Rate: 1.0 mL/min OmniPac PAX-500 Analytical Column (4 × 250 mm).................................... 042152
1 2 4,5 11
Peaks: 1. Orotic Acid 10. Nitrobenzoic Acid OmniPac PCX-100 Analytical Column (4 × 250 mm).................................... 042189
2. 4-Hydroxybenzamide 11. Tribenzylamine
10
15
3. Luminol Impurity 12. p-Nitroaniline OmniPac PCX-500 Analytical Column (4 × 250 mm).................................... 042191
17 4. Luminol 13. 2,4-Dinitroaniline
5. Pyridine 14. Dibenzylamine
8 14 6. PABA 15. N-Methyl-N-nitrosoaniline
3
7. 2,2'-Bipyridine 16. 4-Chloro-2-nitroaniline Guard Columns
8. p-Phenylenediamine 17. 2,6-Dichloro-4-nitroaniline
9. Naphthylamine
0 5 10 15
OmniPac PAX-100 Guard Column (4 × 50 mm)............................................. 042151
Minutes
OmniPac PAX-500 Guard Column (4 × 50 mm)............................................. 042153
5410
OmniPac PCX-100 Guard Column (4 × 50 mm)............................................. 042193
Gradient separation of N-containing compounds. OmniPac PCX-500 Guard Column (4 × 50 mm)............................................. 042195
Separation of a Mixture of Inorganic Cations, Choline, and

Acetylcholine by Two Different Chromatographic Modes
A) Reversed-Phase HPLC
0.2 43 A) Reversed- HPLC Column: C-18
Phase Eluent: 5 mM Heptanesulfonic
AU HPLC acid, pH 4.0/1% Acetonitrile
0.0 Injection: Choline, Acetylcholine (10 mg each)
B) Cation-Exchange IC
0 5 10
Minutes IC Column: OmniPac® PCX-100
Eluent: 75 mM Hydrochloric acid/
LC Columns
2 1 B) Cation- 1% Methanol
3 Exchange Detection: Suppressed conductivity
IC Injection: Choline, Acetylcholine
2
µS (100 ng each)
4 Sodium, Potassium (10 ng each)
0 Peaks (A and B): 1. Sodium
2. Potassium
0 5 10 15 20 3. Choline
Minutes 4. Acetylcholine 7130
Comparison of OmniPac and a reversed-phase column.
Related Literature
Application Notes
AN 106: Ion Chromatography in the Pharmaceutical Industry
AN 65: Analysis of Inositol Phosphates
AN 70: Choline and Acetylcholine
Application Updates
AU 126: Determination of Diethanolamine and Triethanolamine

in Surface Finishing, Wastewater and Scrubber Solutions
AU 133: Saccharin in Electrolytic Nickel Sulfate Baths
www.dionex.com 147
LC Columns
148 www.dionex.com
IC & RFIC Columns
Hydroxide-Selective Anion-Exchange Carbonate Eluent Anion-Exchange

Columns 151 Columns 177
IonPac AS24......................................................... 152 IonPac AS23......................................................... 178

IonPac Fast Anion IIIA......................................... 154 IonPac AS22......................................................... 180

IonPac AS21......................................................... 156 IonPac AS14......................................................... 182

IonPac AS20......................................................... 158 IonPac AS14A...................................................... 184

IonPac AS19......................................................... 160 IonPac AS12A...................................................... 186

IC & RFIC Columns

IonPac AS18......................................................... 162 IonPac AS9-HC.................................................... 188
IonPac AS17-C..................................................... 164 IonPac AS9-SC..................................................... 190

IonPac AS16......................................................... 166 IonPac AS4A-SC.................................................. 192

IonPac AS15......................................................... 168

Ordering Information........................................................ 169 Specialty Anion-Exchange Columns 195
IonPac AS11-HC.................................................. 170 IonPac AS7........................................................... 196

IonPac AS11......................................................... 172 IonPac AS5........................................................... 198

IonPac AS10......................................................... 174

www.dionex.com 149

Cation-Exchange Colums 201 IonPac ICE-Borate................................................ 224
IonPac CS18......................................................... 202 Ordering Information........................................................ 224
IonPac CS17......................................................... 204 IC Trap Columns 225

Ordering Information........................................................ 205 Anion Trap Columns........................................... 226
IonPac CS16......................................................... 206 ATC-3 Anion Trap Column................................................ 226
Related Literature.............................................................. 207 ATC-HC Anion Trap Column............................................ 226
Ordering Information........................................................ 207 ATC-HC Borate Form Anion Trap Column..................... 226
IonPac CS15......................................................... 208 Cation Trap Columns.......................................... 227

Ordering Information........................................................ 209 CTC Cation Trap................................................................. 227
IonPac CS14......................................................... 210 Cation Polisher.................................................... 227

Ordering Information........................................................ 210 Cation Polisher Na+ for Anion Analysis....................... 227
Cation Polisher H+ for Anion Analysis.......................... 228
IonPac CS12A...................................................... 211
MFC-1 Metal-Free................................................ 228
MFC-1 Metal Free Column............................................... 228
IonPac CS11......................................................... 213
IC & RFIC Columns

Ordering Information........................................................ 213 IC Concentrator Columns 229
IonPac CS10......................................................... 214 Anion Concentrator Columns............................ 230
Ordering Information........................................................ 214 UTAC-LP1............................................................................ 230
UTAC-ULP1......................................................................... 230
UTAC-XLP1.......................................................................... 230
TAC-LP1............................................................................... 231
Transition Metal Columns 215
TAC-ULP1............................................................................ 231
TAC-2.................................................................................... 232
IonPac CS5A........................................................ 216
AMC-1.................................................................................. 232
Related Literature.............................................................. 217 AC10..................................................................................... 232
Ordering Information........................................................ 217 AC15..................................................................................... 232
Cryptand C1........................................................................ 233
IonPac TBC-1..................................................................... 233
Ion-Exclusion Columns 219
Cation Concentrator Columns........................... 234
IonPac ICE-AS1.................................................... 220 TCC-2................................................................................... 234
TCC-LP1............................................................................... 234
TCC-ULP1............................................................................ 235
TCC-XLP1............................................................................ 235
IonPac ICE-AS6.................................................... 222
Transition Metal Concentrator Columns........... 236
TMC-1.................................................................................. 236
150 www.dionex.com
Hydroxide-Selective Anion-Exchange Columns
Hydroxide-selective anion-exchange columns
optimized for use with hydroxide eluent
Choose a hydroxide eluent column for use with an eluent gen-
erator for isocratic and gradient analysis. Hydroxide eluents for
isocratic or gradient elution are very convenient with Reagent-
Free Ion Chromatography (RFIC) systems with electrolytic
eluent generation. Hydroxide-selective anion-exchange columns
are available in a wide range of capacities and hydrophobicities.
IonPac AS24: High-capacity anion-exchange column for separa-
RFIC technology simplifies method development for hydroxide- tion of haloacetic acids and bromate in drinking water prior to
gradient systems; the electrolytic eluent generator provides MS or MS/MS detection or 2-D analysis.
gradient methods that are simpler to use than manually-prepared
IonPac Fast Anion IIIA: Hydroxide-selective anion-exchange
isocratic eluents. Modern continuous-eluent-suppression sys-
column designed for the rapid determination of phosphoric and
tems are designed to suppress hydroxide eluents, even at high
citric acids in cola soft drink samples
concentrations.
IonPac AS21: Hydroxide-selective anion-exchange column
for fast analysis of trace perchlorate in drinking water prior to
detection with MS/MS.
IonPac AS20: High-capacity anion-exchange column for de-
termination of trace perchlorate using suppressed conductivity
detection.
IonPac AS19: High-capacity hydroxide-selective column for
determination of oxyhalides and inorganic anions.
IC & RFIC Columns

IonPac AS18: Hydroxide-selective anion-exchange column for
determination of inorganic anions and low-molecular-weight
organic acids.
IonPac AS17-C: Hydroxide-selective anion-exchange column
for fast gradient separation of inorganic anions in high-purity
water matrices.
IonPac AS16: High-capacity hydroxide-selective anion-
exchange column optimized for the determination of highly
polarizable anions.
IonPac AS15: High-capacity anion-exchange column for deter-
mination of trace-level concentrations of inorganic anions and
low molecular weight organic acids.
IonPac AS11-HC: High-capacity anion-exchange column de-
signed to resolve a large number of organic acids and inorganic
anion in complex matrices.
IonPac AS11: Anion-exchange column for fast profiling of inor-
ganic anions and organic acid anions.
IonPac AS10: High-capacity hydroxide-selective, anion-ex-
change column designed for the isocratic and gradient separa-
tion of inorganic anions and organic acids.
www.dionex.com 151
IonPac AS24 Determination of Haloacetic Acids Using the
IonPac® AS24 Column and MS/MS Detection
For separation of haloacetic acids and bromate in
drinking water prior to MS or MS/MS detection 7500
3 KOH Gradient: Conc. (mM)
7.0
Time (min)
0.0
7.0 18.0
Intensity, cps
18.0 36.5
The IonPac AS24 hydroxide-selective anion-exchange column 4 5
6 60.0 36.8
7
is specifically designed for haloacetic acids in drinking water 2 8 9
60.0
7.0
52.8
53.0
1
prior to MS or MS/MS detection. The capacity and selectivity 500 7.0 60.0
0 5 10 15 20 25 30 35 40 45 50 55 60
enables analysis of haloacetic acids in drinking water at low- Minutes Peaks: µg/L (ppb)
µg/L concentrations. The AS24 column is the specified column Column: IonPac AG24, AS24 2 mm 1. Chloroacetic acid 1
Eluent Source: EGC II KOH cartridge 2. Bromoacetic acid 1
in US EPA Method 553. Temperature. 15 °C 3. Dichloroacetic acid 1
Flow Rate: 0.30 mL/min 4. Bromochloroacetic acid 1
Inj. Volume: 100 µL 5. Dibromoacetic acid 1
• Specified column for US EPA Method 553 Suppressor: ASRS® MS, 2 mm 6. Trichloroacetic acid 1
Detector: API 2000, MRM mode 7. Bromodichloroacetic acid 1
Solvent: Acetonitrile, 0.20 mL/min 8. Chlorodibromoacetic acid 1
• High capacity: 140 µeq per column (2 × 250 mm) 9. Tribromoacetic acid 1 24301
• Determine HAAs in high-ionic strength matrices without Determination of haloacetic acids using the IonPac AS24 column and
sample pretreatment MS/MS detection.
• Column selectivity is optimized for a 15 ºC operating

temperature Determination of Haloacetic Acids Using the
IonPac® AS24 Column and MS Detection
• Compatible with HPLC organic solvents
Column: IonPac AS24, 2 mm
Eluent Source: EGC II KOH cartridge
• Used as second dimension column in 2-D method for bro- 100%
1 4 60.00 mM Eluent: KOH gradient
mate in US EPA Method 302.0 Suppressor: ASRS® MS, 2 mm
Relative Abundance 5
Postcolumn
Solvent: Acetonitrile, 0.2 mL/min
Column Temp.: 15 °C
2, 3 6 Inj. Volume: 100 µL
Detector: MSQ™ Plus, -ESI, 400 °C, 3 kV
Peaks (all 100 µg/L): SIM m/z Cone Voltage
18.00 mM
IC & RFIC Columns
0 1. Chloroacetate 93 35
Concentration: 2. Bromoacetate 137 30
7.00 mM
3. 13C-Bromoacetate ISTD 139 35
0 10 20 30 40 48 4. Dichloroacetate 127 30
Minutes 5. Dibromoacetate 216.8 40
6. Trichloroacetate 161 30
24302
Determination of haloacetic acids using the IonPac AS24 column and

MS detection.
152 www.dionex.com
IonPac AS24
Determination of Trace Concentrations of Bromate Using the Related Literature
IonPac® AS24 Column with Two-Dimensional Ion Chromatography
Second-Dimension Conditions
1.5 Column: IonPac AG24, AS24, 2 mm PDF documents under Literature on www.dionex.com.
Eluent Source: EGC II KOH cartridge
Eluent: Potassium hydroxide,
10 mM KOH from 0 to 24 min,
65 mM KOH from 12.1 to 35 min Product Data Sheets
µS ASRS® ULTRA II 2 mm, IonPac AS24 Anion-Exchange Column
AutoSuppression® external water mode
B 1
Current: 41 mA
A Conc. Column: IonPac TAC-ULP1 (5 × 35 mm) Application Notes
Cut Volume: 2 mL
Temperature: 30 °C
0.5 Sample: A) Unfortified Drinking Water B AN 187: Determination of Sub-μg/L Bromate in Municipal and
0 10 20 30 B) Fortified Drinking Water B
A B
Natural Mineral Waters Using Preconcentration with Two-
Minutes
Peaks: 1. Bromate 1.19 1.69 µg/L Dimensional Ion Chromatography and Suppressed Conductivity
24220
Detection
Determination of trace concentrations of bromate using the IonPac
AS24 column with two-dimensional ion chromatography.
Anion Separation Including Inorganic Anions, Organic Acids,
Oxyanions, and Oxyhalides on an IonPac® AS24 Column Using a Analytical Columns
Potassium Hydroxide Eluent Delivered by an Eluent Generator
IonPac AS24 Analytical Column (2 × 250 mm)............................................. 064153
Column: IonPac AG24, AS24 2 mm 18
Eluent Source: EGC II KOH Cartridge 16
6
5 mM to 15 mM KOH 1 9
12
13
14 Guard Columns
µS 3 17
from 0 to 10 min, 1011 15 18 19
8
15 mM to 30 mM KOH 2 45 7 IonPac AG24 Guard Column (2 × 50 mm)..................................................... 064151
from 10 to 20 min,
30 mM to 60 mM KOH –2
from 20 to 38 min 0 4 8 12 16 20 24 28 32 37
Temperature: 15 °C Minutes
Flow Rate: 0.3 mL/min Peaks: mg/L (ppm)
IC & RFIC Columns

Inj. Volume: 5 µL 1. Fluoride 5 8. Nitrite 15 14. Phosphate 40
Detection: Suppressed conductivity, 2. Acetate 15 9. Sulfate 15 15. Arsenate 40
ASRS ULTRA II 2 mm, 3. Formate 15 10. Chlorate 25 16. Thiosulfate 40
AutoSuppression® 4. Chlorite 15 11. Bromide 25 17. Chromate 40
recycle mode 5. Bromate 15 12. Oxalate 25 18. Citrate 40
6. Chloride 15 13. Nitrate 25 19. Isocitrate 40
7. Trifluoroacetate 15 24303
Separation of various anions on an IonPac AS24 column using a potas-

sium hydroxide eluent delivered by an Eluent Generator.
www.dionex.com 153
IonPac Fast Anion IIIA Determination of Phosphoric Acid in a Cola Soft Drink
Rugged and reliable, rapid determination of Using the Fast Anion IIIA Column
phosphoric and citric acids in colas 25

Column: IonPac® Fast Anion IIIA
6
guard and analytical, 3 mm
Eluent: 22 mM potassium hydroxide
This hydroxide-selective anion-exchange column is specifically Eluent Source: EGC II KOH cartridge with CR-ATC
designed for the determination of phosphoric and citric acids in 4
Temperature: 30 °C
cola soft drinks. Its capacity and selectivity allows rapid analysis Inj. Volume: 1.3 µL
µS Detection: Suppressed conductivity,
of these acids in less than 7 minutes. 12
ASRS® ULTRA II, 2 mm
5 Peaks: 1. Unknown — mg/L (ppm)
• Use with the Eluent Generator for simplified Reagent-Free 2. Unknown ––
3. Unknown ––
IC operation. 4. Unknown ––
–5 5. Unknown ––
• The 3 mm i.d. configuration provides economical opera- 0 2 4
Minutes
6 8 6. Phosphate 498
tion. 23286
• Combine with the ASRS 300 suppressor for low back- Determination of phosphoric acid in a cola soft drink using the Fast
ground and enhanced sensitivity. Anion IIIA column.
• Compatible with organic solvents.

Determination of Phosphoric and Citric Acid in a
The Fast Anion IIIA is optimized for a 30 °C operating tempera- Cola Soft Drink Using the Fast Anion IIIA Column
ture to ensure reproducible retention times in all environmental
25 Column: IonPac® Fast Anion IIIA
conditions. It is compatible with organic solvents to enhance Guard and Analytical, 3 mm
5
analyte solubility, modify column selectivity, or allow effective Eluent: 22 mM potassium hydroxide
Eluent Source: EGC II KOH cartridge with CR-ATC
column cleanup. The Fast Anion IIIA column is recommended Temperature: 30 °C
for use with the Eluent Generator, requiring only a deionized Inj. Volume: 1.3 µL
water source to produce potassium hydroxide eluent. 2 ASRS® ULTRA II, 2 mm
1 AutoSuppression recycle mode
3 6 Peaks: 1. Unknown — mg/L (ppm)
IC & RFIC Columns
The cola samples may require sample pretreatment with sonica- 4 2. Unknown ––
3. Unknown ––
tion to remove carbonation. Sample dilution of cola syrups is 4. Unknown ––
–5 5. Phosphate 435
recommended prior to analysis to ensure optimum column life. 0 2 4 6 8 6. Citrate 53
Minutes
These acids can be analyzed in less than 7 minutes using an iso-
23287
cratic potassium hydroxide eluent delivered by an Eluent Gen-
erator in combination with suppressed conductivity detection. Determination of phosphoric and citric acid in a cola soft drink using
the Fast Anion IIIA column.
154 www.dionex.com
IonPac Fast Anion IIIA
Determination of Phosphoric and Citric Acid in a Related Literature

Diet Cola Soft Drink Using the Fast Anion IIIA Column
Column: IonPac® Fast Anion IIIA
Guard and Analytical, 3 mm
16
4 Eluent Source: EGC II KOH cartridge with CR-ATC
Flow Rate: 1.0 mL/min Product Data Sheets
3 Inj. Volume: 1.3 µL
µS
2 8 ASRS® ULTRA II, 2 mm IonPac Fast Anion IIIA Anion-Exchange Column
5 Peaks: 1. Unknown —
6 2. Unknown — Application Updates
3. Unknown —
–2 4. Unknown —
0 2 4 6 8 5. Unknown — AU 153: Fast Determinations of Phosphate and Citrate in Car-
Minutes 6. Unknown —
7. Phosphate 228 mg/L (ppm)
bonated Beverages Using On-Line Degassing with the Carbon-
8. Citrate 175 ate Removal Device (CRD) and a Reagent-Free Ion Chromatog-
23288
raphy System
Determination of phosphoric and citric acid in a diet cola soft drink us-
ing the Fast Anion IIIA column.
Rapid Analysis of Chloride and Sulfate on an

Analytical Columns
IonPac® Fast Anion IIIA Column
IonPac Fast Anion IIIA Analytical Column (3 × 250 mm).............................. 062964
2.5
1
Column: IonPac Fast Anion IIIA
2 guard and analytical, 3 mm Guard Columns
Eluent Source: EGC II KOH cartridge with CR-ATC IonPac Fast Anion IIIA Guard Column (3 × 50 mm)...................................... 062966
Temperature: 30 °C
µS Flow Rate: 1.0 mL/min
ASRS® ULTRA II, 2 mm
IC & RFIC Columns

AutoSuppression® recycle mode
Peaks: 1. Chloride 5 mg/L (ppm)
2. Sulfate 10
–0.5
0 1 2 3 4 5
Minutes
23291
Rapid analysis of chloride and sulfate on an IonPac Fast Anion IIIA

column.
www.dionex.com 155
IonPac AS21 Determination of Perchlorate Using the
IonPac® AS21 and MS/MS Detection
For fast analysis of trace perchlorate in drinking
Column: IonPac AS21, 2 mm Parameter LC-MS/MS
water prior to detection with MS/MS Eluent: 200 mM methylamine Ion Source Electrospray
Temperature: 30 °C Polarity Negative
Flow Rate: 0.35 mL/min Sheath Gas (N2) 80
Inj. Volume: 100 µL Aux Gas (N2) 20
The AS21 hydroxide-selective anion-exchange column is Diversion: Eluent divert valve before detector Capillary Voltage –23 V
specifically designed for determination of trace perchlorate in to send common anions (e.g.,
chloride, bicarbonate, sulfate)
Capillary Temp
Spray Voltage
275 °C
6.00 KV
drinking water prior to MS/MS detection. The capacity and se- to waste (open until 2 min prior to
elution of perchlorate)
Tube Lens
Multipole1 Offset
–35 V
9.00 V
lectivity enable fast analysis of perchlorate at low µg/L concen- Detector: Finnigan LCQ Deca Ion Trap MS Lens Voltage
Multipole2 Offset
68 V
12.50 V
Peaks: 1. Perchlorate 2.0 µg/L (ppb)
trations. The AS21 is the specified column in US EPA Method 1
Multipole RF Amp
Entrance Lens Voltage
120
44.00 V
100
331.0. Coarse Trap dc Offset 10.17 V
Fine Trap dc Offset 10.15 V
Relative Analyzer Temp 26 °C
Abundance Multiplier Voltage –930 V
• Fast separation of perchlorate prior to MS/MS detection SIM m/z 99 83 Mass Range Low
Injection RF m/z 50
0 Collision Energy 52%
0 2 4 6 8 10 12 13
• Optimized for methylamine or hydroxide mobile phases Minutes 21060
• Specified column for US EPA Method 331.0 Determination of perchlorate using the IonPac AS21 and MS/MS detec-
tion.
• Optimum capacity: 45 µeq per column (2 × 250 mm)
• Operates at ambient or elevated temperatures IC/MS Determination of Perchlorate in Drinking Water
Using the IonPac® AS21 Column
• Compatible with organic solvents 1 Column: IonPac® AG21, AS21, 2 mm
600 (A) Eluent: 15 mM potassium hydroxide
µS 2 Eluent Source: EGC II KOH with CR-ATC
The AS21 2 × 250 mm column format was specifically devel- Flow Rate: 0.35 mL/min
oped for MS/MS compatibility to allow use of volatile mobile 8,000 –
Inj. Volume: 100 µL
(B) SIM m/z 99 35Cl16O4 3 Matrix Diversion: 0–6.5 min.
phases such as methylamine. counts
Auxiliary Pump
Solvent: 50/50 acetonitrile/water
0 Auxiliary Pump
3,200 – Detection: (A) Suppressed conductivity,
(B) SIM m/z 101 37Cl16O4 3 ASRS® MS, AutoSuppression®,
counts external water mode
(B) Mass spectrometry
IC & RFIC Columns
0 MS Instrument: MSQ™ Plus

8,000 MS Conditions: –ESI, 70 V, 550 °C
3 Matrix: Drinking Water
– Internal Standard: Perchlorate-18O, 1 µg/L (ppb)
counts (B) SIM m/z 107 35Cl18O4 ISTD*
Peaks: 1. Chloride –
0 2. Sulfate –
0 6 12 3. Perchlorate 1
Minutes *ISTD = Internal Standard 21061
IC/MS determination of perchlorate in drinking water using the IonPac

AS21 column.
156 www.dionex.com
IonPac AS21
Analysis of Environmental Anions Using the IonPac® AS21 Column
Column: IonPac AG21, AS21, 2 mm

7 Eluent: 15 mM potassium hydroxide Analytical Columns
1 Eluent Source: EGC II KOH cartridge with CR-ATC
Temperature: 30 °C IonPac AS21 Analytical Column (2 × 250 mm)............................................. 063009
7 Detection: Suppressed conductivity,
6 ASRS® ULTRA II, 2 mm, Guard Columns
µS 2 3 5 9 AutoSuppression® recycle mode
10 Peaks: 1. Fluoride 2 mg/L (ppm) IonPac AG21 Guard Column (2 × 50 mm)..................................................... 063071
2. Chloride 2
3. Nitrate 5
4 4. Carbonate –
5. Sulfate 5
6. Tungstate 30
–1
7. Chromate 30
0 5 10 15 20
Minutes 8. Perchlorate 30
9. Phosphate 30
10. Arsenate 30
21062
Analysis of environmental anions using the IonPac AS21 column.
Related Literature
Product Data Sheets
IonPac AS21 Anion-Exchange Column Data Sheet
IC & RFIC Columns
www.dionex.com 157
IonPac AS20 Determination of Trace Perchlorate in Drinking Water
Using the AS20 Column Following Concentration
For determination of trace perchlorate using on a Cryptand C1 Concentrator Column
suppressed conductivity detection
Column: IonPac® AG20, AS20, 2 mm
1.58 Conc. Column: IonPac Cryptand C1, 4 × 35 mm
Sunnyvale Drinking Water
The AS20 hydroxide-selective anion-exchange column is spe- Spiked with
Eluent: Sodium hydroxide:
0.5 mM from 0–12 min,
0.5 µg/L Perchlorate
cifically designed for the determination of trace concentrations 65 mM from 12.1–28 min,
100 mM from 28.1–30 min
of perchlorate in drinking water, surface water, and groundwater Eluent Source: EGC II NaOH cartridge
1 with CR-ATC
matrices. The capacity and selectivity of the AS20 ensures that µS
Temperature 35 °C
perchlorate can be quantified at low µg/L concentrations using Sunnyvale
Flow Rate:
Inj. Volume:
0.25 mL/min
2 mL
suppressed conductivity detection even in the presence of very Drinking Water Rinse Volume: 1 mL (10 mM NaOH)
high concentrations of chloride, carbonate, and sulfate. ASRS® ULTRA II, 2 mm,
1.43 AutoSuppression®,
23 24 25 26 27 external water mode, 100 mA
Minutes
• Recommended for US EPA Method 314.1 (Confirmatory Peak: 1. Perchlorate 0.5 µg/L (ppb)
Recovery 107.8%
21089
Method)
• High capacity: 310 µeq per column. (4 × 250 mm) Determination of trace perchlorate in drinking water using the AS20
column following concentration on a Cryptand C1 Concentrator
• Use the Cryptand C1 Concentrator Column for sample column.
preconcentration
System Flow Path for Trace Perchlorate in Drinking Water
• Simplified Reagent-Free IC operation provided by the Elu-
ent Generator Load Sample: 2 mL
Autosampler
Rinse Matrix: 1 mL of 10 mM NaOH
Eluent Generation
• ASRS 300 provides RFIC operation with low background 0.25 mL/min

Steps 1, 2, and 3 Load, Rinse
and enhanced analyte sensitivity Inject External Water
Waste
• Column selectivity is optimized for 30 °C operating tem- Cryptand C1

Concentrator
perature Column Waste
Guard Analytical ASRS 300 Conductivity

• Compatible with organic solvents
IC & RFIC Columns
Waste Column Column Suppressor Detector
Primary Method—AG16, AS16 (2 250 mm)

The AS20 is the specified column in US EPA Method 314.1 NaOH Eluent Generation
Confirmatory Method—AG20, AS20 (2 250 mm)
Step Function Conc. Time
(Confirmatory Method). The Cryptand C1 Concentrator Column 1 Perchlorate Transfer 0.5 mM 12 min
is the specified concentrator column for sample preconcentra- 2 Analysis 65 mM 16 min
3 Column Cleanup 100 mM 2 min 20590
tion in that method.
System flow path for trace perchlorate in drinking water.
The AS20 column is ideally used with an RFIC system for best
detection limits. Use the Anion Self-Regenerating Suppressor
(ASRS 300) with the AS20 column for eluent suppression.
158 www.dionex.com
IonPac AS20
Gradient Separation of a Variety of Environmental Anions Related Literature
Using the AS20 Column
Column: IonPac® AG20, AS20, 4 mm Peaks: mg/L (ppm) 12. Carbonate 20 For detailed specifications and applications, see the following
Eluent: Potassium hydroxide: 1. Fluoride 2 13. Sulfate 10
5 mM from 0 to 5 min, 2. Acetate 10 14. Selenate 10 PDF documents under Literature on www.dionex.com.
5–30 mM from 5 to 15 min, 3. Butyrate 10 15. Oxalate 10
30–55 mM from 15 to 30 min 4. Formate 10 16. Phthalate 20
Eluent Source: EGC II KOH with CR-ATC 5. Chlorite 10 17. Phosphate 20
6. Bromate 10 18. Chromate 20
Temperature:
Flow Rate:
30 °C
1.0 mL/min 7. Chloride 5 19. Iodide 20 Product Data Sheets
Inj. Volume: 10 µL 8. Nitrite 10 20. Arsenate 20
Detection: Suppressed conductivity, 9. Chlorate 10 21. Citrate 20
ASRS® ULTRA II, 4 mm, 10. Bromide 10 22. Thiocyanate 20 IonPac AS20 Anion-Exchange Column Data Sheet
AutoSuppression® recycle mode 11. Nitrate 10 23. Perchlorate 30
10
13
7
8
15
Application Notes
4 14 20
17
10 11 18
16 22
µS 1
2 5
6
9
19
21 23 AN 176: Determining Sub-ppb Perchlorate in Drinking Water
3 12 Using Preconcentration/Matrix Elimination IC with Suppressed
–1
0 5 10 15 20 25 30
Conductivity Detection by U.S. EPA Method 314.1
Minutes 21114
AN 178: Improved Determination of Trace Concentrations of

Gradient separation of a variety of environmental anions using the Perchlorate in Drinking Water Using Preconcentration with
AS20 column.
Two-Dimensional Ion Chromatography and Suppressed Con-
ductivity Detection
Comparison of the AS16 and AS20 Columns
for Separation of Common Anions,
Hydrophobic Anions and 4-Chlorobenzene Sulfonate Ordering Information
Columns: (A) IonPac® AG16, AS16, 2 mm
12 (A) Aromatic Backbone
4 (B) IonPac AG20, AS20, 2 mm
1 23 6 Eluent: 35 mM sodium hydroxide
5
7, 8
Eluent Source: EGC II NaOH cartridge Analytical Columns
with CR-ATC
–1 Detection: Suppressed conductivity, IonPac AS20 Analytical Column (4 × 250 mm)............................................. 063148
ASRS® ULTRA II, 2 mm,
12 (B) Aliphatic Backbone AutoSuppression® recycle mode
Peaks: 1. Fluoride 2 mg/L (ppm)
1 3
Guard Columns
IC & RFIC Columns

2 4 2. Chloride 3
6 3. Sulfate 5
µS 5 8 4. Thiosulfate 10
5. Iodide 20 IonPac AG20 Guard Column (2 × 50 mm)..................................................... 063066
7 6. Thiocyanate 20
–1 7. 4-Chlorobenzene IonPac AG20 Guard Column (4 × 50 mm)..................................................... 063154
0 2 4 6 8 10 12 14 16 18 20 22 25 sulfonate 5
Minutes 8. Perchlorate 30 21090
Comparison of the AS16 and AS20 columns for separation of common

anions, hydrophobic anions and 4-chlorobenzene sulfonate.
www.dionex.com 159
IonPac AS19 Separation of Oxyhalides and Common Inorganic Anions
For determination of oxyhalides and inorganic Column: IonPac® AG19, AS19, 4 × 250 mm
anions 10
from 0 to10 min,
10 to 55 mM from 10 to 25 min
40
Eluent source: EGC-KOH cartridge with CR-ATC
The IonPac AS19 hydroxide-selective anion-exchange column Flow rate: 1.0 mL/min
Temperature: 30 °C
is specifically designed for trace bromate in drinking water. Its 8
Suppressor: ASRS® ULTRA, 4 mm,
11 External water mode, 300 mA
high capacity and selectivity allow the determination of bromate 7 Inj. volume: 25 µL
1 5 6 Peaks: 1. Fluoride 1 mg/L (ppm)
in drinking water at the low-µg/L level. The AS19 meets the per- µS
2. Chlorite 10
3 4
formance requirements of US EPA Methods 300.0 and 300.1. 2
3. Bromate
4. Chloride
20
6
9
5. Nitrite 15
6. Chlorate 25
• Recommended hydroxide-selective column for trace bro- 7. Bromide 25
–5
mate in drinking water matrices 0 5 10 15 20 25 30
8. Nitrate
9. Carbonate —
25
Minutes 10. Sulfate 25

• High capacity: 240 µeq per column (4 × 250 mm) 11. Phosphate 30 20283
• Meets or exceeds performance requirements of US EPA Determination of oxyhalides and common inorganic anions using the
Methods 300.0 and 300.1 AS19 column.
• Column selectivity is optimized for a 30 °C operating

temperature Determination of ppb Bromate in Drinking Water
Column: IonPac® AS19, 4 × 250 mm
5 11
• Compatible with organic solvents 300 Eluent: Potassium hydroxide:
10 mM from 0 to 10 min,
10 to 45 mM from 10 to 25 min,
• Low backgrounds and enhanced analyte sensitivity with the 9
Eluent source: EGC II KOH cartridge with CR-ATC
µS
ASRS 300 and an RFIC system 1
2 34 6 78
Temperature: 30 °C
Suppressor: ASRS® ULTRA II, 4 mm,
10 12
AutoSuppression external water mode,
–50 300 mA
The AS19 is designed for the analysis of oxyhalides and com- Inj. volume: 500 µL
1 5 6 9 10, 11 12 Peaks: 1. Fluoride 1 mg/L (ppm)
mon inorganic anions including fluoride, chlorite, bromate, 0.3
2. Formate –
3. Chlorite 0.005
chloride, nitrite, bromide, chlorate, nitrate, phosphate, and 4. Bromate 0.005
IC & RFIC Columns
µS 5. Chloride 50
sulfate in drinking water, groundwater, wastewater, and other 78 6. Nitrite 0.005
diverse sample matrices. The AS19 allows analysis of most 2 34 7. Chlorate 0.005
8. Bromide 0.005
–0.03 9. Nitrate 10
drinking water without the use of sample pretreatment or pre- 0 5 10 15 20 25 30 10. Carbonate 25
concentration. The AS19 column is ideally used with an RFIC 12. Phosphate 0.2
20282-a
system for automatic eluent generation.
Determination of trace concentration of bromate in a simulated drink-
The key application for the AS19 is the determination of trace ing water sample using the AS19 column with a large-loop injection.
bromate in drinking water matrices using a potassium hydroxide
gradient with suppressed conductivity detection. The selectivity
of the AS19 ensures that bromate, a toxic byproduct of ozone
disinfection, can be quantified at low µg/L concentrations using
suppressed conductivity detection even in the presence of very
high concentrations of chloride, sulfate, and carbonate.
Note: Use the Anion Self-Regenerating Suppressor (ASRS

300) with the AS19 column for eluent suppression.
160 www.dionex.com
IonPac AS19
Determination of Inorganic Anions, Oxyhalides, Organic Acids, Application Updates

and Oxyanions on an IonPac® AS19 Column Using a Potassium
Hydroxide Gradient Delivered by an EG50 Eluent Generator
AU 154: Determination of Bromate in Drinking and Mineral
40 Water by Isocratic Ion Chromatography with a Hydroxide Elu-
12
14 15
ent
µS 10 17
3 6 7 8 9 19 20 21 22
1 2 45 13 16 18 23
11 AU 159: Determination of Volcanic Gases as Anions in Caustic
–5
0 5 10 15 20
Minutes
25 30 35 40 45 Solutions Using AutoNeutralization, Automated Dilutions, and
Column: IonPac AG19, AS19, 4 mm Peaks: 1. Fluoride 2 mg/L (ppm) 12. Sulfate 25 a Reagent-Free Ion Chromatography System
Eluent: Potassium hydroxide: 2. Acetate 10 13. Malonate 25
10 mM from 0 to 10 min, 3. Formate 10 14. Selenate 25
10–58 mM from 10 to 40 min 4. Chlorite 10 15. Oxalate 25
Eluent source: EGC II KOH with CR-ATC 5. Bromate 20 16. Iodide 30
Flow rate: 1.0 mL/min 6. Chloride 6 17. Thiosulfate 25
Temperature: 30 °C 7. Nitrite 15 18. Chromate 25
Suppressor: ASRS® ULTRA II, 4 mm, 8. Chlorate 25 19. Phosphate 30
AutoSuppression recycle mode, 9. Bromide 25 20. Fumarate 30
300 mA 10. Nitrate 25 21. Arsenate 30
Inj. volume: 25 µL 11. Carbonate 25 22. Thiocyanate 30 Analytical Columns
23. Perchlorate 30
20284
Determination of inorganic anions, oxyhalides, organic acids and IonPac AS19 Analytical Column (2 × 250 mm)............................................. 062886
oxyanions on an IonPac AS19 column using a hydroxide gradient deliv-
ered by an Eluent Generator
Guard Columns
IonPac AG19 Guard Column (4 × 50 mm)..................................................... 062887
Related Literature IonPac AG19 Guard Column (2 × 50 mm)..................................................... 062888

Product Data Sheets
IC & RFIC Columns

Application Notes
AN 93: Determination of Trace Anions in Concentrated Bases

Using AutoNeutralization Pretreatment/Ion Chromatography
AN 167: Determination of Trace Concentrations of Oxyhalides

and Bromide in Municipal and Bottled Waters Using a Hydrox-
ide-Selective Column with a Reagent-Free Ion Chromatography
System
AN 168: Determination of Trace Concentrations of Disinfec-

tion By-Product Anions and Bromide in Drinking Water Using
Reagent-Free Ion Chromatography Followed by Postcolumn
Addition of o-Dianisidine for Trace Bromate Analysis
AN 171: Determination of Disinfection By-Product Anions and

Bromide in Drinking Water Using a Reagent-Free Ion Chroma-
tography System Followed by Postcolumn Addition of an Acidi-
fied On-Line Generated Reagent for Trace Bromate Analysis
AN 184: Determination of Trace Concentrations of Chlorite,

Bromate, and Chlorate in Bottled Natural Mineral Waters
AN 187: Determination of Sub-μg/L Bromate in Municipal and

Natural Mineral Waters Using Preconcentration with Two-
Dimensional Ion Chromatography and Suppressed Conductivity
Detection
www.dionex.com 161
IonPac AS18 Fast Isocratic Anion Standard Separation
For determination of inorganic anions and low- Using the IonPac® AS18 Column
molecular-weight organic acids 12
Column: IonPac AS18 4 mm
Eluent: 39 mM Potassium hydroxide
Eluent Source: EGC-KOH with CR-ATC
The IonPac AS18 anion-exchange column is the hydroxide- 34 Temperature: 30 °C
5 6
selective column of choice for compliance monitoring of Flow Rate:
Inj. Volume:
1.0 mL/min
25 µL
1
2
inorganic anions in drinking water and wastewater samples in µS
ASRS® ULTRA 4 mm, 150 mA,
accordance with US EPA Methods 300.0 (A) and 300.1. AutoSuppression® recycle mode
2. Chloride 2
• Recommended hydroxide-selective column for inorganic 3. Nitrite 5
4. Sulfate 6
anions in diverse sample matrices 5. Bromide 10
-1 6. Nitrate 10
0 2 4 6 8 10 7. Phosphate 20
• Fast isocratic separation of common inorganic anions in Minutes
9 min 19081
• Inorganic anion and low-molecular weight organic acids in Fast isocratic separation of the common inorganic anions on an IonPac
complex sample matrices AS18.
• Superior retention and quantification of fluoride, acetate,

and formate Determination of Anions in Drinking Water
• Meets performance requirements specified in US EPA Column: IonPac AS18, 4 × 250 mm
4.0
Method 300.0 (A) 5 10 11
22 to 40 mM at 7–8 min
Temperature: 30 °C
The AS18 can determine inorganic anions and low-molecular- 8
weight organic acids in a variety of sample matrices. This Detection: Suppressed conductivity,
column has excellent retention of fluoride from the water dip. µS
ASRS® ULTRA 4-mm, 100 mA
1
It is approved for compliance monitoring of inorganic anions in 9 Peaks: 1. Fluoride 8. Carbonate
drinking water and wastewater samples in accordance with US 2 6 2. Acetate 9. Bromide
IC & RFIC Columns
3 12 13 3. Unknown 10. Sulfate

7
EPA Methods 300.0 and 300.1. 4
4. Formate 11. Nitrate
5. Chloride 12. Chlorate
-0.5 6. Nitrite 13. Phosphate
-0.2 5 10 15 20 7. Unknown
Minutes 18903
Determination of inorganic anions in a drinking water sample on an

IonPac AS18 column using a potassium hydroxide gradient delivered
by an Eluent Generator.
162 www.dionex.com
IonPac AS18
Fast Dual-Gradient Separation of Wastewater Related Literature
Column: IonPac AS18 4 mm For detailed specifications and applications, see the following
20 Eluent: Potassium hydroxide:
8 12 18
12–44 mM from 0–5 min PDF documents under Literature on www.dionex.com.
and 44–52 mM from 8–10 min
Temperature: 30 °C
Inj. Volume: 100 µL Product Data Sheets
2 ASRS® ULTRA 4 mm, 150 mA,
µS AutoSuppression® recycle mode IonPac AS18 Anion-Exchange Columns Data Sheet
Sample Prep: Wastewater sample diluted 1:100
3
15
Peaks: 1. Fluoride
2. Acetate
11. Sulfite
12. Sulfate
Application Notes
5 3. Formate 13. Bromide
10
46 14 1617 4. Unknown 14. Unknown
1 7 9
13
19 5. Unknown 15. Nitrate AN 154: Determination of Inorganic Anions in Environmental
6. Unknown 16. Unknown
-2 7. Unknown 17. Unknown
Waters Using a Hydroxide-Selective Column
8. Chloride 18. Thiosulfate
0 2 4 6 8 10 12 14
9. Nitrite 19. Unknown
Minutes
10. Carbonate 19055 AN 156: Determination of Anions in Toothpaste by Ion Chro-
matography
Determination of inorganic anions in a wastewater sample on an
IonPac AS18 column using a potassium hydroxide gradient delivered AN 165: Determination of Benzoate in Liquid Food Products by
by an Eluent Generator. Reagent-Free Ion Chromatography
Fast Dual-Gradient Separation of Environmental Application Updates

Anions Using the IonPac® AS18 Column
14
5
AU 146: Determination of Anions in Acid Rain by Ion Chroma-
Peaks: 1. Fluoride 0.5 mg/L
9 11
10 12 2. Acetate 2.5 tography
6 3. Formate 1.0
7 4. Chlorite 5.0
µS 13
14 5. Chloride 3.0
8 17 19
15 16 18
1 6. Nitrite 6.0
23 4
7. Selenite 10.0
8. Sulfite 10.0
9. Sulfate 10.0
-2
0 2 4 6 8 10 12 14 10. Bromide 10.0
Minutes 11.
12.
Selenate
Nitrate
10.0
10.0 Analytical Columns
IC & RFIC Columns

Column: IonPac AS18 4 mm
13. Chlorate 10.0
Eluent: Potassium hydroxide:
12–44 mM from 0–5 min
14.
15.
Phosphate
Molybdate
10.0
10.0
IonPac AS18 4 mm Analytical Column (4 × 250 mm)................................... 060549
and 44–52 mM from 8–10 min
16. Tungstate 10.0
17. Arsenate 10.0 IonPac AS18 2 mm Analytical Column (2 × 250 mm)................................... 060553
Temperature: 30 °C
18. Thiosulfate 10.0
19. Chromate 10.0
Inj. Volume: 25 µL
Detection: Suppressed conductivity, ASRS® ULTRA 4 mm, 150 mA,
AutoSuppression® recycle mode 19054
Guard Columns
IonPac AG18 4 mm Guard Column (4 × 50 mm)........................................... 060551
Fast dual-gradient separation of environmental anions using the Ion-
Pac AS18 column. IonPac AG18 2 mm Guard Column (2 × 50 mm)........................................... 060555
www.dionex.com 163
IonPac AS17-C Sulfur Speciation Using the IonPac AS17-C
Column: IonPac AG17-C, AS17-C, 4 mm
For fast gradient separation of inorganic anions in Eluent: Potassium hydroxide,
4 7 8 9 1 mM from 0–1.5 min,
high-purity water matrices 6 1–20 mM from 1.5–5 min,
20–40 mM from 5–7 min
Eluent Source: EGC II KOH with CR-ATC
The IonPac AS17-C column is a low-capacity column for fast, Temperature: 30 °C
gradient separation of inorganic anions. Its key application is Sample Prep: Diluted 1:100
Inj. Volume: 10 µL
the determination of common inorganic anions in high-purity Detection: Suppressed conductivity,
µS
water matrices. The AS17-C provides low sulfate blanks and ASRS ULTRA II,
AutoSuppression recycle mode
3
fast equilibration time. It is recommended for use with RFIC 6 mg/L (ppm)
Peaks: 1, 2. Acetate, Glycolate 2.0, 2.0
systems using the Eluent Generator for automatic eluent genera- 1,2
5 3. Formate 2.0
tion. Use the Anion Self-Regenerating Suppressor (ASRS 300) 4.
5.
Chloride
Nitrate
20.0
1.0
with the AS17-C column. 0 6. Carbonate 50.0
7. Sulfite 4.0
0 2 4 6 8 8. Sulfate 40.0
• Recommended replacement for all AS17 anion applications Minutes 9. Thiosulfate 12.0 23771
• Optimized for common inorganic anions in simple sample Determination of sulfur species in a simulated industrial wastewater
sample using an IonPac AS17-C Column
matrices
• Fast gradient separation of inorganic anions in high-purity
Anions in HPW on the AS17-C
water matrices 2.0 Column: IonPac® AG17-C, AS17-C, 4 mm
Eluent: Potassium hydroxide; 1 mM from
• Fast, gradient separation of sulfur species including sulfite, 0–10 min, 1–12 mM from 10–14 min,
12–20 mM from 14-20 min
sulfate, and thiosulfate Eluent Source: EGC II KOH with CR-ATC
• Compatible with RFIC systems and the Eluent Generator Detection: Suppressed conductivity,
ASRS® ULTRA II, AutoSuppression®
for automatic eluent generation µS
11 recycle mode
Temperature: 30 °C
Sample: Deionized water spiked with anions
Note: The IonPac AS18 column is the recommended Peaks: 1. Fluoride
µg/L (ppb)
1 9. Nitrate 5
7
hydroxide-selective column for determination of common 2. Acetate 10 10. Benzoate 20
IC & RFIC Columns
9 13 3. Formate 10 11. Carbonate —

3 12 14
anions in diverse sample matrices. 2
4 5 6 8
10
15
4. Acrylate
5. Methacrylate 10
10 12. Sulfate
13. Oxalate
5
10
1
6. Chloride 5 14. Phthalate 10
7. Nitrite 5 15. Phosphate 10
–0.1 8. Bromide 5
0 5 10 15 20 25 30
Minutes
23668
Determination of anions and organic acids in high-purity water using

a large loop injection with potassium hydroxide gradient on a 4 mm
IonPac AS17-C column.
164 www.dionex.com
IonPac AS17-C
Anions in Toothpaste on the AS17-C Ordering Information
9 10 IonPac® AG17-C, AS17-C, 4 mm

Column:
5.0 11 Eluent:
Potassium hydroxide; 1 mM from Analytical Columns
0–3 min, 1–12 mM from 3–11 min,
12–35 mM from 11–14 min
1 Eluent Source: EGC II KOH with CR-ATC IonPac AS17-C Analytical Column (4 × 250 mm)......................................... 066294
Inj. Volume: 10 µL IonPac AS17-C Analytical Column (2 × 250 mm)......................................... 066296
µS ASRS® ULTRA II, AutoSuppression®
8 recycle mode
Temperature: 30 °C
Guard Columns
Sample: Toothpaste diluted 200× (w/w)
with deionized water and filtered IonPac AG17-C Guard Column (4 × 50 mm).................................................. 066295
23 7 Peaks: 1. Fluoride 7. Benzoate
45 6
–0.1 2. Acetate 8. Carbonate IonPac AG17-C Guard Column (2 × 50 mm).................................................. 066297
0 2 4 6 8 10 12 14 16 18 3. Formate 9. Monofluorophosphate
Minutes 4. Chloride 10. Sulfate
5. Nitrite 11. Phosphate
6. Bromide
23669
Determination of anionic additives in toothpaste on an IonPac AS17-C

column using a potassium hydroxide gradient delivered by an Eluent
Generator.
Separation of Environmental Anions Using the IonPac AS17-C

Column: IonPac AG17-C, AS17-C, 4 mm Temperature: 30 °C
Eluent: Potassium hydroxide, Flow Rate: 1.5 mL/min
1 mM from 0 to 3 min, Inj. Volume: 10 µL
1–12 mM from 3 to 10 min, Detection: Suppressed conductivity,
12–35 mM from 10 to 14 min ASRS ULTRA II,
Eluent Source: EGC II KOH with CR-ATC AutoSuppression recycle mode
mg/L (ppm)
Peaks: 1. Fluoride 2.0 9. Bromide 10.0
6
2. Acetate 5.0 10. Nitrate 10.0
13 3. Propionate 5.0 11. Chlorate 10.0
4. Formate 5.0 12. Carbonate 20.0
10 5. Chlorite 5.0 13. Sulfate 5.0
4 14 6. Bromate 5.0 14. Oxalate 5.0
IC & RFIC Columns

µS 7 8 15
1 9 7. Chloride 3.0 15. Phosphate 10.0
11
8. Nitrite 5.0
5
2 6
3 12
0
0 5 10 15
Minutes 23769
Anion separation including oxyhalides on an IonPac AS17-C column

using a potassium hydroxide gradient delivered by an Eluent Genera-
tor.
Related Literature
Product Data Sheets
IonPac AS17-C Anion Exchange Column Data Sheet
www.dionex.com 165
IonPac AS16 Isocratic Separation of Polarizable Anions
High-capacity column optimized for determination of and Inorganic Anions Using a Sodium Hydroxide Eluent
polarizable anions Column: IonPac AG16, AS16, 4 mm
Eluent: 35 mM sodium hydroxide
10
Temperature:30 °C
The IonPac AS16 column is ideally suited for trace perchlorate 1 Flow Rate: 1 mL/min
23
in drinking water in accordance with US EPA Methods 314.0 4
Inj. Volume:10 µL
and 314.1 (Primary Method). The AS16 column simplifies 5 ASRS ULTRA II, 4 mm,
µS 7
the determination of polarizable anions, including thiosulfate, AutoSuppression recycle mode
Peaks: 1. Fluoride 2.0 mg/L (ppm)
iodide, thiocyanate, and perchlorate using an isocratic hydroxide
2. Chloride 3.0
eluent. 3. Sulfate 5.0
0 4. Thiosulfate 20.0
5. Iodide 20.0
• Separate hydrophobic anions (iodide, thiocyanate, and 0 5 10
Minutes
15 20
6. Thiocyanate 20.0
thiosulfate) in less than 20 min 7. Perchlorate 20.0
14228-01
• Can be used for trace perchlorate in drinking water matrices
Isocratic separation of polarizable anions and inorganic anions using
• Ideal for polyvalent anions, including polyphosphates and a sodium hydroxide eluent on the 4 mm IonPac AS16 column.
polycarboxylates
• Ultralow hydrophobicity allows fast analysis of polarizable Determination of Trace Perchlorate in Drinking Water
Using the AS16 Column Following Concentration
anions
on a Cryptand C1 Concentrator Column
The AS16 is a high-capacity, hydroxide-selective column for 2.2 Sunnyvale Drinking Water Conc. Column: IonPac Cryptand C1, 4 35 mm
Spiked with 0.5 µg/L Perchlorate
the determination of polarizable anions in a variety of sample Eluent: Sodium hydroxide:
0.5 mM from 0–12 min,
matrices. Trace concentrations of perchlorate in drinking water, 65 mM from 12.1–28 min,
100 mM from 28.1–30 min
surface water, and groundwater matrices can easily be deter- 1 Eluent Source: EGC II NaOH cartridge with CR-ATC
Temperature 35 °C
mined using a large-loop injection. µS
Inj. Volume: 2 mL
Rinse Volume: 1 mL (10 mM NaOH)
Note: Also see the IonPac AS20, with complementary
IC & RFIC Columns

Sunnyvale ASRS® ULTRA II, 2 mm,
Drinking Water
selectivity for confirmation of perchlorate identification when 1.9
AutoSuppression®,
external water mode, 100 mA
using EPA Method 314.1 23 24 25
Minutes
26 27 Peaks: 1. Perchlorate 0.5 µg/L (ppb)
Recovery 116.5%
21088
Determination of trace perchlorate in drinking water using the AS16

column.
166 www.dionex.com
IonPac AS16
Gradient Separation of Polarizable Anions and Inorganic Anions AN 151: Determination of Perchlorate in Environmental Waters
Using the IonPac® AS16 and EG50 Eluent Generator by Ion Chromatography Coupled with Electrospray Mass Spec-
16 12 trometry (IC-MS)
µS
8 17 AN 176: Determining Sub-ppb Perchlorate in Drinking Water
7 9 13 16 19
20
1 23
4 5 11 10 14 15 18 Using Preconcentration/Matrix Elimination IC with Suppressed
6
0
Conductivity Detection by U.S. EPA Method 314.1
0 5 10 15 20 25 30
Minutes
mg/L (ppm) AN 178: Improved Determination of Trace Concentrations of
Column: IonPac AG16, AS16, 4 mm Peaks: 1. Fluoride 2.0 11. Carbonate 20.0
Eluent: Potassium hydroxide, 2. Acetate 10.0 12. Sulfate 10.0 Perchlorate in Drinking Water Using Preconcentration with
3. Propionate 10.0
1.5 mM from 0 to 7 min to
10 mM at 13 min 4. Formate 10.0
13.
14.
Selenate
Iodide
10.0
20.0
Two-Dimensional Ion Chromatography and Suppressed Con-
5. Chlorite 10.0
Eluent Source: EGC-KOH
Temperature: 30 °C 6. Bromate 10.0
15.
16.
Thiosulfate
Chromate
20.0
20.0
ductivity Detection
7. Chloride 5.0
Flow Rate: 1.5 mL/min 17. Phosphate 20.0
8. Nitrite 10.0
Inj. Volume: 10 µL 18. Arsenate 20.0
9.
10.
Nitrate
Selenite
10.0
10.0 19. Thiocyanate 20.0 Application Updates
ASRS® ULTRA, 4 mm, 20. Perchlorate 20.0
AutoSuppression® recycle mode 14230-01
AU 145: Determination of Perchlorate in Drinking Water by Ion
Determination of polarizable anions and inorganic anions using a Chromatography
potassium hydroxide gradient delivered with an EG using the 4 mm
IonPac AS16 column. AU 148: Determination of Perchlorate in Drinking Water Using
Reagent-Free™ Ion Chromatography
Separation of Polyphosphates in Detergent
Using the IonPac® AS16 Column Ordering Information
10
(A) Polyphosphate Standard Column: IonPac AG16, AS16, 4 mm
Eluent: 25 mM potassium hydroxide for
4 6 1.7 min to 65 mM in 2.5 min
µS
1 3 Eluent Source: EG40 Analytical Columns
5 7 8 9 Temperature: 30 °C
2 Inj. Volume: 10 µL IonPac AS16 Analytical Column (4 × 250 mm)............................................. 055376
Detection: Suppressed Conductivity,
0 ASRS® ULTRA, 4 mm, IonPac AS16 Analytical Column (2 × 250 mm)............................................. 055378
10 7
(B) Dishwashing detergent Peaks: A
sample, 300 mg/L 1. Chloride 3 mg/L (ppm)
IC & RFIC Columns

2. Carbonate - Guard Columns
3. Sulfate 5
µS 1 4. Phosphate 10
5. Pyrophosphate 10 IonPac AG16 Guard Column (4 × 50 mm)..................................................... 055377
2 3 4 9 6. Trimetaphosphate 10
5
0 7. Tripolyphosphate 10 IonPac AG16 Guard Column (2 × 50 mm)..................................................... 055379
8. Tetrametaphosphate 10
0 5 10 9. Tetrapolyphosphate 10
Minutes 14238
Separation of polyphosphates on the 4 mm IonPac AS16 column using

a potassium hydroxide gradient delivered with an Eluent Generator.
Related Literature
Product Data Sheets
Application Notes
AN 134: Determination of Low Concentrations of Perchlorate

in Drinking and Ground Waters Using Ion Chromatography
AN 138: Determination of Thiosulfate in Refinery and Other

Wastewaters
AN 144: Determination of Perchlorate in High Ionic Strength

Fertilizer Extracts By Ion Chromatography
www.dionex.com 167
IonPac AS15 Comparison of IonPac® AS15-5µm and AS15-9µm Columns
for Isocratic Separation of Common Anions
For trace-level concentrations of inorganic anions Column: (A) IonPac AG15,
AS15-5mm (3 × 150 mm)
and low-molecular weight organic acids 10
1 4 A
(B) IonPac AG15,
2 5 AS15-9mm (4 × 250 mm)
67 Eluent: (A) 42 mM potassium hydroxide
The IonPac AS15 column was designed specifically for analysis µS 8
(B) 40 mM potassium hydroxide
Eluent Source: EG40
of trace anions in high-purity water matrices, for the semicon- Temperature: 30 °C
3 Flow Rate: (A) 0.5 mL/min
ductor and power generation industries. Its high capacity and 0 (B) 1.2 mL/min
selectivity enable the determination of trace-level concentrations Inj. Volume: (A) 5 µL
(B) 10 µL
10
of inorganic anions and low-molecular-weight organic acids in B
ASRS® ULTRA,
1
high-purity water matrices. µS 2 4 5 AutoSuppression® recycle mode
6 7 mg/L
8
3 Peaks: 1. Fluoride 2 5. Sulfate 10
• AS15-5 µm (3 × 150 mm) column for high efficiency, fast 0 2. Chloride 5 6. Bromide 20
3. Carbonate – 7. Nitrate 20
analysis (15 min) 0 5 10 15 20 25 4. Nitrite 10 8. Phosphate 30
Minutes
16122
• AS15-9 µm (2 × 250 and 4 × 250 mm) columns for higher-
capacity applications Comparison of the AS15-5µm (3 × 150 mm) and AS15-9µm
(4 × 250 mm) for the separation of inorganic anions.
• Column selectivity optimized for 30 °C operating tempera-
ture Determination of Inorganic Anions and Low-Molecular-Weight
Organic Acids with Sample Preconcentration
• Superior resolution of early-eluting anions (fluoride, glyco- Using the IonPac® AS15 Column
late, acetate, and formate)
4.0 Concentrator
6 Column: IonPac AC15
This column is ideal for use with large-loop injections. Use the Eluent: Potassium hydroxide,
AC15 concentration column for ultratrace (ppt) analyses. Use 7 10–40 mM from 4 min to 14 min,
µS 40–60 mM from 14 to 18 min
with the Eluent Generator for simplified eluent preparation. Use 1
4 8 Eluent Source: EG40
9 11 Temperature: 30 °C
the Anion Self-Regenerating Suppressor (ASRS 300) with this 23 5 10
Sample Volume: 20 mL, preconcentrated
column. 0
ASRS® ULTRA, 2 mm,
IC & RFIC Columns
0 5 10 15 20 AutoSuppression® recycle mode

Minutes µg/L (ppb)
Peaks: 1. Fluoride 0.1 7. Sulfate 0.1
2. Acetate 0.1 8. Oxalate 0.1
3. Formate 0.1 9. Bromide 0.1
4. Chloride 0.1 10. Nitrate 0.1
5. Nitrite 0.1 11. Phosphate 0.1
6. Carbonate –
14871
Determination of inorganic anions and low-molecular-weight organic

acids with sample preconcentration using the IonPac AS15 column.
168 www.dionex.com
IonPac AS15
Separation of Weakly Retained Organic Acids
Using the IonPac® AS15-5µm (3 x 150 mm) Column
4.0 17
Analytical Columns
4 6 1112 15
1 10 13 14 16
µS 23 7
5 8 9 IonPac AS15 Analytical Column (4 × 250 mm)............................................. 053940
0
0 2 4 6 8 10 12 14 16
Minutes
mg/L (ppm)
IonPac AS15-5µm Analytical Column (3x150 mm)....................................... 057594
Column: IonPac AG15, Peaks: 1. Fluoride 1 10. Sulfite 2
AS15-5mm (3 × 150 mm) 2. Glycolate 5 11. Sulfate 2
Eluent: 10 mM Potassium hydroxide 3. Acetate 5 12. Oxalate 3 Guard Columns
for 4 min to 60 mM at 9 min 4. Formate 5 13. Bromide 5
Eluent Source: EG40 5. Propionate 5 14. Nitrate 5
Temperature: 30 °C 6. Chloride 2 15. Phosphate 10 IonPac AG15 Guard Column (4 × 50 mm)..................................................... 053942
Flow Rate: 0.7 mL/min 7. Nitrite 2 16. Chlorate 10
Inj. Volume: 5 µL 8. Butyrate 5 17. Thiosulfate 10 IonPac AG15 Guard Column (2 × 50 mm)..................................................... 053943
Detection: Suppressed conductivity, 9. Carbonate 30
ASRS® ULTRA, 2 mm, IonPac AG15-5µm Guard Column (3x30 mm)............................................... 057597
AutoSuppression® recycle mode 16393
Separation of weakly-retained organic acids using a potassium hydrox-

ide gradient on the IonPac AS15-5 µm (3 × 150 mm) column.
Related Literature
Product Data Sheets
IC & RFIC Columns

Application Notes
AN 137: Determination of Trace Anions in High-Nitrate Matri-

ces by Ion Chromatography

Bromide in Drinking Water Using a Reagent-Free Ion Chroma-
tography System Followed by Postcolumn Addition of an Acidi-
fied On-Line Generated Reagent for Trace Bromate Analysis
AN 173: Direct Determination of Cyanide in Drinking Water

by Ion Chromatography with Pulsed Amperometric Detection
(PAD)
AN 179: Carbohydrate and Amino Acid Analysis Using 3-D

Amperometry
Application Updates
AU 142: Improved Determination of Trace Anions in High Pu-

rity Waters by High-Volume Direct Injection with the EG40
AU 143: Determination of Chloride in Acid Copper Plating

Bath
Technical Notes
TN 48: Determination of Trace Anions in High-Purity Water by

High-Volume Direct Injection with the EG40
www.dionex.com 169
IonPac AS11-HC Gradient Separation of Organic Acids and Inorganic Anions
Using the IonPac AS11 Column
For resolving a large number of inorganic anions and 10 14
18 1920
22 23
25 26 28 29 31
6 8 12 15
organic acid anions in complex matrices µS 2 4
7 10
9 13
11
17
16
21 24 27
30
1 3 5
0
0 5 10 15 20 25 30 35 40
Complex sample matrices such as chemical wastewater effluents Minutes
Column: IonPac AG11-HC, Peaks: 1. Quinate 10 16. Trifluoroacetate 10
and fermentation broth solutions contain a variety of inorganic AS11-HC, 4-mm 2. Fluoride 3 17. Bromide 10
Eluent: Sodium hydroxide, 3. Lactate 10 18. Nitrate 10
anions and organic acids. The IonPac AS11-HC was specifi- 1 mM from 0 to 8 min, 4. Acetate 10 19. Carbonate 20
1 mM to 30 mM 5. Propionate 10 20. Malonate 15
cally designed to resolve a large number of inorganic anions and from 8 to 28 min, 6. Formate 10 21. Maleate 15
30 mM to 60 mM 7. Butyrate 10 22. Sulfate 15
organic acid anions in a single run using a hydroxide gradient. from 28 to 38 min 8. Methylsulfonate 10 23. Oxalate 15
Flow Rate: 1.5 mL/min 9. Pyruvate 10 24. Ketomalonate 20
Temperature: 30 °C 10. Chlorite 10 25. Tungstate 20
11. Valerate 10 26. Phosphate 20
• Use for organic acids and anions in complex sample matri- Inj. Volume: 10 µL
Detection: Suppressed conductivity, 12. Monochloro- 27. Phthalate 20
28. Citrate 20
ces or uncharacterized samples. ASRS® ULTRA 4-mm,
AutoSuppression®
acetate
13. Bromate
10
10 29. Chromate 20
recycle mode 14. Chloride 5 30. cis-Aconitate 20
15. Nitrite 10 31. trans-Aconitate 20
• Recommended for monovalent and divalent organic acids. 13485A
• Use the AS11 for fast analysis of organic acids and anions Determination of organic acids and inorganic anions using the IonPac
in well-characterized samples. AS11-HC column.
• Use the ICE-AS1 or ICE-AS6 columns for organic acids in

high-ionic-strength samples. Separation of Organic Acids and Inorganic
Anions in Wine Using the IonPac® AS11-HC Column
• High capacity translates into longer retention times, but
with higher resolution. 10 9 10
12 15
Column: IonPac AG11-HC, AS11-HC, 4 mm
4 14 17 1 mM from 0 to 8 min,
µS 1 5
8 18
The high-capacity AS11-HC column allows the injection of 2
6
7 11 13 16 1 mM to 30 mM from 8 in 28 min,
30 mM to 60 mM from 28 in 38 min;
3
more concentrated samples without overloading or peak broad- 0 Methanol: 20% from 18 to 28 min
Temperature: 30 °C
ening, and provides improved separation over the AS11 column 0 5 10 15 20 25
Minutes
30 35 40
Sample Prep: Diluted 1:10,
for monovalent carboxylic acids, including quinate, lactate, Peaks: 1. Lactate 10. Tartrate treated with OnGuard® RP
acetate, propionate, formate, and butyrate. 2. Acetate 11. Fumarate Inj. Volume: 10 µL
IC & RFIC Columns
3. Formate 12. Sulfate Detection: Suppressed conductivity,

4. Pyruvate 13. Oxalate ASRS® ULTRA, 4 mm,
5. Galacturonate 14. Phosphate AutoSuppression® external water mode
6. Chloride 15. Citrate
7. Nitrate 16. Isocitrate
8. Succinate 17. cis-Aconitate
9. Malate 18. trans-Aconitate 13489
Separation of organic acids and inorganic anions in wine using the

IonPac AS11-HC column.
170 www.dionex.com
IonPac AS11-HC
Separation of Organic Acids and Inorganic Anions Application Notes
in Apple Juice Using the IonPac® AS11-HC Column
Column: IonPac AG11-HC, AS11-HC, 4 mm
AN 123: The Determination of Inorganic Anions and Organic
10
Acids in Fermentation Broths
8
1 mM to 15 mM from 8 to 18 min,
15 mM to 30 mM from 18 in 28 min,
30 mM to 60 mM from 28 in 38 min;
AN 143: Determination of Organic Acids in Fruit Juices
Methanol: 18% from 14 to 38 min
Temperature: 30 °C
µS
1 11
13
Flow Rate: 1.5 mL/min Technical Notes
Sample Prep: Diluted 1:10
9 12 14
6 10
2
3 4
5 Detection: Suppressed conductivity, TN 44: The Determination of Trace Anions in Concentrated
0 ASRS® ULTRA, 4 mm,
AutoSuppression® external water mode Phosphoric Acid
Peaks: 1. Quinate 8. Malate
0 5 10 15 20 25 30 35 40 2. Lactate 9. Sulfate
Minutes 3. Acetate
4. Formate
10. Oxalate
11. Phosphate
TN 46: Determination of Trace Anions in Concentrated Gly-
5. Galacturonate 12. Citrate colic Acid
6. Chloride 13. Isocitrate
7. Succinate 14. cis-Aconitate
13490
Separation of organic acids and inorganic anions in apple juice using
the IonPac AS11-HC column.
Analytical Columns
Separation of Anions in Beer Using the IonPac AS11 Column IonPac AS11-HC Analytical Column (4 × 250 mm)....................................... 052960
Column: IonPac AG11-HC, AS11-HC, 4 mm Flow Rate: 1.5 mL/min IonPac AS11-HC Analytical Column (2 × 250 mm)....................................... 052961
Eluent: Sodium hydroxide: 1 mM from 0 to 8 min, Temperature: 30 ˚C
1 mM to 15 mM from 8 to 18 min, Sample Prep: Diluted 1:5, treated with OnGuard-RP
15 mM to 30 mM from 18 to 28 min, Inj. Volume: 10 µL
30 mM to 60 mM from 28 to 38 min; Detection: Suppressed conductivity, Guard Columns
Methanol: 18% from 14 to 38 min ASRS-ULTRA 4 mm, AutoSuppression
6 external water mode
10
15 IonPac AG11-HC Guard Column (4 × 50 mm)............................................... 052962
Peaks: 1. Lactate 11. Carbonate
2. Acetate 12. Fumarate IonPac AG11-HC Guard Column (2 × 50 mm)............................................... 052963
3. Formate 13. Sulfate
4. Butyrate (2 ppm) 14. Oxalate
µS 5. Pyruvate 15. Phosphate
10
13 19 6. Chloride 16. Citrate
4 16 7. Bromide
IC & RFIC Columns

12 18 17. Isocitrate
1 7
9 8. Nitrate 18. cis-Aconitate
2 17
5 8 11 14 9. Succinate 19. trans-Aconitate
3 10. Malate
0
0 5 10 15 20 25 30 35 40
Minutes 13488
IonPac AS11-HC column used for the analysis of beer spiked with
2 mg/L (ppm) butyrate.
Related Literature
Product Data Sheets
IonPac AS11 and AS11-HC Anion-Exchange Columns Data

Sheet
www.dionex.com 171
IonPac AS11 Determination of Organic Acids and Inorganic Anions
For fast profiling of inorganic anions and organic 5 22 23
121314 15 21
acid anions µS
10 11
18
20 25 27
24 26 28 29 31
2 8 9 17
19 30
4 6 16
1 3 5 7
The AS11 is a relatively low-capacity column designed for fast, 0
0 5 10 15 20 25
Minutes
gradient screening of inorganic anions and organic acid anions Peaks: 1. Quinate 2.5 mg/L (ppm) 16. Trifluoroacetate 2.5 Column: IonPac AG11,
2. Fluoride 0.75 17. Bromide 2.5 AS11, 4-mm
in simple sample matrices. 3. Lactate 2.5 18. Nitrate 2.5 Eluent: Sodium hydroxide,
4. Acetate 2.5 19. Carbonate 5 0.2 mM from 0 to 6 min,
5. Propionate 2.5 20. Malonate 3.75 0.2 mM to 5 mM
21. Maleate 3.75
• Fast analysis of organic acids and inorganic anions in well- 6. Formate
7. Butyrate
2.5
2.5 22. Sulfate 3.75
from 6 to 11 min,
5 mM to 38 mM
8. Methyl- 23. Oxalate 3.75 from 11 to 23 min
characterized samples sulfonate 2.5 24. Ketomalonate 5 Flow Rate: 2.0 mL/min
9. Pyruvate 2.5 25. Tungstate 5 Temperature: 30 °C
10. Chlorite 2.5 26. Phosphate 5 Inj. Volume: 10 mL
• Ideal for highly charged anions, including polyphosphates 11. Valerate 2.5 27. Phthalate 5 Detection: Suppressed conductivity,
12. Monochloro- 28. Citrate 5 ASRS® ULTRA 4-mm,
and polycarboxylates acetate 2.5 29. Chromate
30. cis-Aconitate
5
5
AutoSuppression®
13. Bromate 2.5 recycle mode
14. Chloride 1.25 31. trans-Aconitate 5
13485-B
• For organic acids and inorganic anions in complex sample 15. Nitrite 2.5
matrices Determination of organic acids and inorganic anions using the IonPac
AS11 column.
• Low capacity translates into fast analysis times and high
throughput
Separation of Polarizable Anions Using the IonPac® AS11 Column
The AS11 is designed for fast profiling of inorganic anions
and organic acid anions in foods, beverages, chemical process 6 7
Column:
Eluent:
IonPac AS11, 4 mm
45 mM sodium hydroxide
solutions, wastewater, brines, and power plant waters. The AS11 in 40% methanol
34 8 Flow Rate: 1 mL/min
column can resolve a large number of inorganic anions and 2 6 Inj. Volume: 25 µL
organic acids in approximately 15 minutes. 1
ASRS® ULTRA, 4 mm
µS 5 AutoSuppression®
external water mode
9 Peaks: 1. Fluoride 2 mg/L (ppm)
2. Chloride 2
3. Nitrate 5
IC & RFIC Columns
4. Sulfate 5
0
5. Phosphate 10
6. Iodide 20
0 2 4 6 8 10
7. Thiocyanate 20
Minutes 8. Thiosulfate 20
9. Perchlorate 20
8510-A
Separation of polarizable anions using the IonPac AS11 column.
172 www.dionex.com
IonPac AS11
Separation of Polyphosphates in Detergent AN 107: Ions In Physiological Fluids
AN 112: Determination of Nitrate and Nitrite in Meat Using
Column:
IonPac AS11, 4 mm
16 1 3 (A) Polyphosphate Standard Eluent:
20 mM to 80 mM High-Performance Anion-Exchange Chromatography
6 Sodium hydroxide in 10 min
4 5 7 Flow Rate: 2.0 mL/min
µS
8
9
Detection: Suppressed conductivity, AN 113: Determination of Trace Anions in High Purity Waters
ASRS® ULTRA, 4 mm,
2
AutoSuppression® recycle mode by High Volume/Direct Injection Ion Chromatography
(A) Peaks: 1. Chloride 3 mg/L (ppm)
2. Carbonate 3
0
3. Sulfate 5 AN 114: Determination of Trace Anions in High-Purity Waters
4. Phosphate 10
30 2
(B) Detergent Sample 160 mg/L (ppm)
5. Pyrophosphate 10 Using Direct Injection and Two-Step Isocratic Ion Chromatog-
6. Trimetaphosphate 10
7. Tripolyphosphate 10 raphy
6 8. Tetrapolyphosphate 10
µS 9. Tetrametaphosphate 10
1 3 4 5 (B) Peaks: 1. Carbonate AN 116: Quantification of Anions in Pharmaceuticals
2. Sulfate
3. Phosphate
0
0 2 4 6 8
4. Unknown
5. Pyrophosphate
AN 121: Analysis of Low Concentrations of Perchlorate in
Minutes 6. Tripolyphosphate 8676-04 Drinking Water and Ground Water by Ion Chromatography
Separation of polarizable anions in detergent using the IonPac AS11 AN 123: The Determination of Inorganic Anions and Organic
column. Acids in Fermentation Broths
AN 161: Determination of Metal Cyanide Complexes by Ion

Separation of Common Fermentation Broth Anions
Using the IonPac AS11 Column Chromatography with On-Line Sample Preconcentration and
UV Absorbance Detection
Column: IonPac® AS11, AG11 Guard Inj. Volume: 10 µL
Eluent: 0.5-38 mM Sodium hydroxide Temperature: Ambient
Sample: 10 mg/L each analyte
AN 164: Assay for Citrate and Phosphate in Pharmaceutical
Formulations
14 Peaks: 1. Lactate 13. Nitrate
2. Acetate 14. Malate
10 3. Propionate 15.
16.
Methylmalonate
Carbonate
Application Updates
18 4. Formate
19 5. 2-Keto-D-Gluconate 17. Malonate
17 20
6. Pyruvate 18. Maleate AU 122: The Determination of Iodide in Brine
µS 13 15 7. Valerate 19. Sulfate
4 9 12 14 8. Monochloroacetate 20. Oxalate
2
IC & RFIC Columns

22
1 3 56
8 11
23 9. Bromate 21. Trichloroacetate AU 140: The Determination of Iodide in Urine
24 10. Chloride 22. Phosphate
25
7 21 11. Phenylacetate 23. Citrate
12. Bromide 24. Isocitrate AU 147: Direct Determination of Metal Cyanides by Ion Chro-
0 25. Pyrophosphate
0 5 10 15 20 matography with UV Absorbance Detection
Minutes 13618
AU 149: Determination of Metal Cyanide Complexes in Solid
Yeast fermentation broth culture (10-fold dilution) analyzed using the Wastes by Anion-Exchange Chromatography with UV Absor-
IonPac AS11. bance Detection
Technical Notes
Related Literature
TN 48: Determination of Trace Anions in High-Purity Water by
For detailed specifications and applications, see the following High-Volume Direct Injection with the EG40
Product Data Sheets
IonPac AS11 and AS11-HC Anion-Exchange Columns Data Analytical Columns

Sheet
Application Notes IonPac AS11 Analytical Column (2 × 250 mm)............................................. 044077
AN 37: The Determination of Iodide in Milk Products Guard Columns

AN 71: Determination of Polyphosphates Using Ion Chroma- IonPac AG11 Guard Column (4 × 50 mm)..................................................... 044078
tography with Suppressed Conductivity Detection IonPac AG11 Guard Column (2 × 50 mm)..................................................... 044079

AN 104: Analysis of Personal Care Products by Ion Chromatog-

raphy
www.dionex.com 173
IonPac AS10 Gradient Separation of Organic Acids and Inorganic Anions
For isocratic and gradient separation of inorganic
14
anions and organic acids 3
7
Column:
Eluent:
IonPac® AS10, 4-mm
50–124 mM Sodium hydroxide
in 30 min
8
The IonPac AS10 is a high-capacity hydroxide-selective, anion- 2 9 Inj. Volume: 20 µL
Detection: Suppressed conductivity
exchange column designed for isocratic and gradient separation µS 6
of inorganic anions and organic acids. The column provides 4
5 10
11
2. Acetate 30
12
excellent resolution of weakly retained low-molecular-weight 3. Formate 5
4. Selenite 10
aliphatic acids. The high capacity of the AS10 permits the 5. Chloride 1.8
6. Nitrite 9
analysis of trace-level analytes in high-ionic-strength matrices. 0 7. Sulfate 10
8. Oxalate 10
0 10 20 30 9. Selenate 10
• Use the AS10 column for analysis of inorganic anions in Minutes
10. Phosphate 9
11. Bromide 10
high nitrate samples 12. Nitrate 6
7438
The column provides excellent resolution of weakly retained

Separation of common organic and inorganic anions on the IonPac
low-molecular-weight aliphatic acids. The selectivity and high AS10 using a hydroxide gradient.
capacity of the AS10 permits the analysis of trace-level analytes
in high-ionic-strength matrices. The AS10 resin strongly retains
nitrate, which makes this column well suited for the analysis of Analysis of Methyldiethanolamine for Corrosive Anions
trace anions in nitric acid and nitrate salts.
2
50
Column: IonPac® AG10, AS10, 4-mm
Use with the Eluent Generator for simplified eluent prepara- Eluent: 100 mM Sodium hydroxide
tion for applications using less than 100 mN hydroxide. Use Detection: Suppressed conductivity
Inj. Volume: 25 µL
the Anion Self-Regenerating Suppressor (ASRS 300) with the µS Sample: Lean MDEA, 1:10 dilution
AS10 column. 1
Peaks: 1. Acetate
2. Formate
48.0 mg/L (ppm)
54.0
4 5 3. Carbonate –
3 4. Chloride 5.9
6 5. Sulfate 12.0
IC & RFIC Columns
0 6. Oxalate 3.5
0 5 10 15 20
Minutes
12370-01
Analysis of methyldiethanolamine for corrosive anions using the Ion-

Pac AS10 column.
174 www.dionex.com
IonPac AS10
Determination of Aliphatic Organic Acids

in Amine Matrix on the IonPac® AS10
5
Analytical Columns
Column: IonPac AG10, AS10, 4 × 250 mm
Eluent: 7 mM Sodium tetraborate
Sample: Standards IonPac AS10 Analytical Column (4 × 250 mm)............................................. 043118
4 Inj. Volume: 25 µL IonPac AS10 Analytical Column (2 × 250 mm)............................................. 043123
µS 3
1 2 Peaks: Guard Columns
1. Acetate 10 mg/L (ppm)
2. Propionate 20
3. Butyrate 40 IonPac AG10 Guard Column (4 × 50 mm)..................................................... 043119
4. Formate 10
0
0 2 4 6 14
Minutes
11873
Determination of aliphatic organic acids in amine matrix on the Ion-

Pac AS10.
Anions from Molten Glass
30 1 6 Column: IonPac® AG10, AS10 (2-mm)

Eluent: 10 mM Sodium hydroxide,
gradient 10–200 mM Sodium hydroxide
from 7 to 27 min
µS
Inj. Volume: 10 µL
4 Sample: Molten glass in 0.8% Hydrofluoric acid,
3 5 1.3% Nitric acid, diluted 1:10 in water
2
0 Peaks: 1. Fluoride – mg/L (ppm)
2. Chloride 2.3
IC & RFIC Columns

0 10 20 30 40 3. Carbonate –
Minutes 4. Sulfate 12.0
5. Phosphate 16.8
6. Nitrate –
14423
Anions in molten glass using the IonPac AS10 column.
Related Literature
Product Data Sheets
IonPac AS10 Anion-Exchange Column and the IonPac AC10

Concentrator Column Data Sheet
Application Notes
AN 78: Determination of Trace Anions in Concentrated Hydro-

fluoric Acid
AN 85: Determination of Trace Anions in Organic Solvents
Technical Notes

colic Acid
www.dionex.com 175
IC & RFIC Columns
176 www.dionex.com
Carbonate Eluent Anion-Exchange Columns
Anion-exchange columns optimized for use with a
carbonate/bicarbonate eluent
Dionex carbonate eluent columns provide well-characterized
isocratic separations, including regulated methods for drinking
and wastewater. Carbonate eluent columns are available in a
wide range of capacities and are compatible with RFIC-EG and
RFIC-ER systems.
IonPac AS23: High-capacity, carbonate eluent anion-exchange

column for the analysis of oxyhalides and the common inor-
ganic anions.
IonPac AS22: High capacity and resolution, carbonate eluent
anion-exchange column recommended for the fast, isocratic
separation of inorganic anions.
IonPac AS14: Moderate-capacity, carbonate eluent anion-
exchange column designed for the fast, isocratic separation of
inorganic anions.
IonPac AS14A: High-capacity, carbonate eluent anion-exchange
column designed for the fast, isocratic separation of inorganic
anions.
IonPac AS12A: Carbonate eluent anion-exchange column
designed for the fast separation of inorganic anions offering
IC & RFIC Columns

excellent retention of fluoride
IonPac AS9-HC: High-capacity carbonate eluent anion-ex-
change column for the analysis of inorganic anions and oxyha-
lides.
IonPac AS9-SC: Carbonate eluent anion-exchange column
designed for the fast, isocratic separation of inorganic anions
and oxyhalides.
IonPac AS4A-SC: Carbonate eluent anion exchange column
for the fast, isocratic separation of inorganic anions in drinking
water and wastewater.
www.dionex.com 177
IonPac AS23 Determination of Oxyhalides and Inorganic Anions
Precise, isocratic analysis of trace oxyhalides and the Using the IonPac® AS23 Column
common inorganic anions 10 1 4 5

8 Column:
Eluent:
IonPac AG23, AS23, 4mm
4.5 mM Sodium carbonate/
7 10
6 0.8 mM Sodium bicarbonate
Temperature: 30 °C
The AS23 carbonate eluent anion-exchange column is specifi- 2
cally designed for trace bromate in drinking water. Its high Inj. Volume: 25 µL
capacity and selectivity enables determination of bromate in µS
9 ASRS® ULTRA II 4 mm,
drinking water at the low µg/L level. The AS23 meets the per- Peaks 1. Fluoride 3 mg/L (ppm)
2. Chlorite 10
formance requirements of U.S EPA Methods 300.0 and 300.1. 3. Bromate 20
4. Chloride 6
5. Nitrite 10
• Optimized for isocratic carbonate/bicarbonate eluent 6. Chlorate 25
–1 7. Bromide 25
0 5 10 15 20 25 8. Nitrate 25
• Recommended column for trace bromate in drinking water Minutes 9. Phosphate 40
10. Sulfate 30
matrices 22947
• Meets performance requirements specified in US EPA Determination of oxyhalides and inorganic anions on an IonPac AS23
Methods 300.0 and 300.1 column.
• Ideal alternative for AS9-HC oxyhalide and inorganic anion

Analysis of Municipal Drinking Water Using
applications
the IonPac® AS23 Column
• Simple, accurate eluent preparation with the AS23 Eluent 1 4 8 10 13
Column:
Eluent:
0.3
Concentrate 0.8 mM sodium bicarbonate
Temperature: 30 °C
2 11 Flow Rate: 1.0 mL/min
• Optimized for a 30 °C operating temperature to ensure µS 6
3 579
12
reproducible retention times ASRS® ULTRA II 4 mm,
–0.1 AutoSuppression® external water mode
• Compatible with organic solvents to enhance analyte solu- 180
Peaks [mg/L (ppm)]:
1. Fluoride 0.96 10. Nitrate 0.54
bility 4 2. Formate NQ 11. Carbonate NQ
3. Chlorite 0.01 12. Phosphate 0.14
IC & RFIC Columns
µS 5 6 78 9 4. Chloride 9.54 13. Sulfate 21.24

13
The AS23 is designed for the analysis of oxyhalides and the 5. Unknown NQ 14. Unknown NQ
1 6. Nitrite 0.01
common inorganic anions including fluoride, chlorite, bromate, 23 10 11 12 14 7. Unknown NQ NQ: Not Quantified
–20 8. Chlorate 0.19
chloride, nitrite, bromide, chlorate, nitrate, phosphate, and 0 5 10 15 20 25 30 35 9. Bromide 0.01
Minutes
sulfate in drinking water, ground water, wastewater, and other 22950
diverse sample matrices.

Determination of oxyhalides and inorganic anions in a municipal
drinking water sample using an IonPac AS23 column.
The AS23 is the newest carbonate eluent column recommended
for the analysis of oxyhalides including bromate. It can be used
in combination with the Eluent Generator and the Electrolytic
pH Modifier (EPM) which automatically produces potassium
carbonate/bicarbonate eluents from water. The AS23 is an ideal
alternative, using carbonate-bicarbonate eluents, for AS9-HC
applications.
178 www.dionex.com
IonPac AS23
Analysis of a Chemical Plant Wastewater Sample Ordering Information

2.0
123 5 8 9 Column:
Eluent:
Analytical Columns
0.8 mM sodium bicarbonate
µS
Inj. Volume: 2.5 µL IonPac AS23 Analytical Column (4 × 250 mm)............................................. 064149
4 6 7 ASRS® ULTRA II 4 mm,
–0.2
Peaks: 1. Acetate 877.8 mg/L (ppm)
Guard Columns
60
2. Formate 183.2
8 3. Chloride 61.8 IonPac AG23 Guard Column (2 × 50 mm)..................................................... 064143
4. Nitrite 0.6
µS 5. Chlorate 213.8 IonPac AG23 Guard Column (4 × 50 mm)..................................................... 064147
12 6. Nitrate 1.5
3 5 7. Phosphate 1.4
4 6 7 9 8. Sulfate 1211.1
–5 9. Unknown NQ
0 5 10 15 20 25 30 35 NQ: Not Quantified
Minutes 22948
Separation of inorganic anions, oxyhalides, and organic acids in a

chemical wastewater sample using an IonPac AS23 column.
Determination of Trace Concentrations of

Bromate Using the IonPac® AS23 Column
Column: IonPac AG23, AS23, 4mm
1 4 5 8 11 Eluent: 4.5 mM Sodium carbonate/
0.3
Temperature: 30 °C
9 10 Inj. Volume: 200 µL
23 67
Detection: Suppressed Conductivity,
ASRS® ULTRA II 4 mm,
–0.1 AutoSuppression® external water mode
500
2. Chlorite 0.01
4
3. Bromate 0.005
4. Chloride 50
IC & RFIC Columns

µS 5. Nitrite 0.1
11
6. Chlorate 0.01
1 5 67 8 9 10 7. Bromide 0.01
–50 8. Nitrate 10
0 5 10 15 20 25 30 9. Carbonate 50
Minutes 10. Phosphate 0.1
11. Sulfate 50 22949
Determination of trace concentrations of bromate using the IonPac

AS23 column with a large-loop injection.
Related Literature
Product Data Sheets
IonPac AS23 Datasheet
Application Notes
AN 184: Determination of Trace Concentrations of Chlorite,

Bromate, and Chlorate in Bottled Natural Mineral Waters
www.dionex.com 179
IonPac AS22 Determination of Inorganic Anions in a Municipal
Drinking Water Sample Using the IonPac® AS22 Column
Fast analysis of common inorganic anions
1 3 11 Eluent: 4.5 mM Sodium carbonate/
0.5 8
Designed specifically for compliance monitoring of inorganic 1.4 mM sodium bicarbonate
Temperature: 30 °C
anions in accordance with US EPA Method 300.0 (A) and µS
Inj. Volume: 25 µL
6 10
300.1, the IonPac AS22 represents the culmination of more than 2 45 7 9 Detection: Suppressed conductivity,
ASRS® ULTRA II 4 mm,
20 years of column development, and is a key complement to Peaks:
1. Fluoride 0.84 mg/L (ppm)
–0.2
our award-winning Reagent-Free IC system. 45
2. Formate
3. Chloride
0.03
15.59
3
4. Nitrite 0.01
5. Unknown NQ
• Fast isocratic separation of the common inorganic anions µS
6. Chlorate 0.18
11 7. Bromide 0.02
in 8 minutes 12
8. Nitrate 0.89
4 56 7 8 10 9. Carbonate NQ
–5 10. Phosphate 0.22
• Isocratic separation of inorganic anions in complex sample 0 5 10 14 11. Sulfate 20.45
Minutes
matrices in 12 minutes NQ: Not Quantified 22941
• Carbonate peak well-resolved from the common inorgainc Common inorganic anions in a municipal drinking water sample can
anions be separated in approximately 12 min.
• Meets performance requirements specified in US EPA

Method 300.0 (A) Determination of Inorganic Anions in Carbonated
Drinking Water Using the IonPac® AS22 Column
• Ideal alternative for AS4A-SC, AS12A, AS14, and AS14A 0.6 2
7
inorganic anion applications 1 Column: IonPac AG22, AS22, 4 mm
4 Eluent: 4.5 mM Sodium carbonate/
• Simple, accurate eluent preparation with the AS22 Eluent µS 5 Temperature: 30 ˚C
Concentrate 3 6
Inj. Volume: 10 µL
–0.2 ASRS® ULTRA II 4 mm,
• Optimized for a 30 °C operating temperature to ensure AutoSuppression® recycle mode
40
reproducible retention times 2 Peaks: 1. Fluoride 0.49 mg/L (ppm)
2. Chloride 61.38
IC & RFIC Columns
3. Bromide 0.19
The AS22 is designed for the determination of inorganic anions µS 4. Nitrate 2.53
5. Carbonate NQ
and low molecular weight organic acids including fluoride, ac- 1 7
6. Phosphate 0.17
3 4 5 6 7. Sulfate 4.4
etate, formate, chloride, nitrite, bromide, nitrate, phosphate, and –5 NQ: Not Quantified
0 5 10 13
sulfate. The AS22 can be used with isocratic carbonate/bicar- Minutes 22942
bonate eluents and suppressed conductivity detection. Common

inorganic anions can easily be separated in a variety of sample The unique selectivity of the AS22 column positions carbonate well
away from the common inorganic anions.
matrices including drinking water, wastewater, process streams,
and scrubber solutions.
The AS22 can be used in combination with the Eluent Genera-

tor and the Electrolytic pH Modifier (EPM) which automatically
produce potassium carbonate/bicarbonate eluents from water.
The AS22 column is the newest carbonate eluent column rec-
ommended for fast analysis of inorganic anions and is an ideal
alternative, using carbonate-bicarbonate eluents, for AS4A-SC,
AS12A, AS14, and AS14A inorganic anion applications.
Note: Use the Anion Self-Regenerating Suppressor (ASRS

300) with the AS22 column for eluent suppression.
180 www.dionex.com
IonPac AS22
The IonPac® AS22 Permits Fast Anion Analysis
7
Column: IonPac AG22, AS22, 2 mm Analytical Columns
Eluent: 4.5 mM Sodium carbonate/
Temperature: 30 °C
1
6
Inj. Volume: 2.5 µL IonPac AS22 Analytical Column (4 × 250 mm)............................................. 064141
5 8
µS
7
AutoSuppression® recycle mode Guard Columns
2 2. Acetate 20
3. Chloride 10 IonPac AG22 Guard Column (2 × 50 mm)..................................................... 064135
4. Nitrite 15
5. Bromide 25 IonPac AG22 Guard Column (4 × 50 mm)..................................................... 064139
6. Nitrate 25
–1
0 1 2 3 4 5 6 7 8 7. Phosphate 40
22943
The IonPac AS22 resin packing supports fast anion analysis.
Separation of Environmental Anions Using

the IonPac® AS22 Column
Inj. Volume: 10 µL
89
1 12 AutoSuppression® recycle mode
µS mg/L (ppm)
2 10 15
14 Peaks: 1. Fluoride 3 10. Phosphate 40
13 2. Acetate 20 11. Sulfate 30
17
16 18 3. Formate 10 12. Selenate 30
4. Bromate 20 13. Iodide 40
IC & RFIC Columns

5. Chloride 5 14. Arsenate 30
–1 6. Nitrite 15 15. Thiocyanate 40
0 10 20 30 7. Chlorate 25 16. Perchlorate 40
Minutes 8. Bromide 25 17. Thiosulfate 40
9. Nitrate 25 18. Chromate 40
22944
Separation of 18 environmental anions is completed in under 30 min-

utes using the IonPac AS22 column.
Related Literature
Product Data Sheets
IonPac AS22 Datasheet
Application Updates
AU 161: Determination of Sulfate and Chloride in Ethanol Us-

ing Ion Chromatography
www.dionex.com 181
IonPac AS14
Analysis of Municipal Drinking Water Using the IonPac® AS14
For the analysis of fluoride and other inorganic
anions
3 6 Column: IonPac AG14, AS14, 4 mm
0.5 Eluent: 3.5 mM Sodium carbonate
The IonPac AS14 is designed for fast analysis of the common Flow Rate:
1.0 mM Sodium bicarbonate
1.2 mL/min
inorganic anions in diverse sample matrices. Inj. Volume: 10 µL
ASRS® ULTRA, 4 mm,
µS
• Common inorganic anions are resolved in 13 minutes using 2 4 5 Sample:
Drinking water from Milpitas, CA
an isocratic carbonate/bicarbonate eluent. 1 Peaks: 1. Fluoride 0.03 mg/L (ppm)
2. Bicarbonate -
3. Chloride 3.12
• Sodium tetraborate gradient optimizes difficult separations. 0 4. Nitrate 0.15
0 2 4 6 8 10 12 14 5. Phosphate 0.04
Minutes
• The AS14 column meets or exceeds US EPA Method 6. Sulfate 4.45
300.0 (A) performance requirements.

12125
The AS14 supports the separation of inorganic anions, includ- The IonPac AS14 column is ideal for interference-free determination of
ing fluoride, chloride, nitrite, bromide, nitrate, phosphate, and inorganic anions, including fluoride, in drinking water.
sulfate using a carbonate/bicarbonate eluent coupled with sup-
pressed conductivity detection. With the AS14, inorganic anions
Sodium Tetraborate Gradient Separation of Anions
can be determined easily in drinking water, wastewater, foods
and beverages, scrubber solutions, and other diverse sample
matrices. 12 Column: IonPac AG14, AS14, 4 mm
Eluent: 2 mM Sodium tetraborate for
6 min; 10-min gradient to 17.5 mM
10 Sodium tetraborate
For simplified operation, use the AS14 Eluent Concentrate and Flow Rate: 1.5 mL/min
Inj. Volume: 25 µL
the Combined Seven Anion Standard. Use the Anion Self-Re- µS Detection: Suppressed conductivity,
9
generating Suppressor (ASRS 300) with the AS14 column for 1 3
4
5 6
7 8 ASRS® ULTRA, 4 mm,
2
low-noise operation. Peaks: 1. Fluoride 5 mg/L (ppm)
2. Glycolate 10
3. Acetate 20
IC & RFIC Columns
0
4. Formate 10
Note: See also the IonPac AS22, the latest column 0 2 4 6 8 10 12 14 16 18
5. Chloride 3
6. Nitrite 10
recommended for fast analysis of inorganic anions. Minutes 7. Bromide 10
8. Nitrate 10
9. Phosphate 15
10. Sulfate 15 12123-01
Separation of weakly retained anions using a sodium tetraborate gradi-

ent elution.
182 www.dionex.com
IonPac AS14
Application Notes
Crude and GPC-Purified In-Process Peptide
Using the IonPac® AS14 Column AN 115: Determination of Trifluoroacetic Acid (TFA) in Pep-
-1.5 Crude
Columns: IonPac AG14, AS14, 4 mm
tides
10 µL of 40 µg/mL
3
µS Flow Rate:
1.2 mL/min
AN 116: Quantification of Anions in Pharmaceuticals
ASRS® ULTRA, 4 mm,
AN 133: Determination of Inorganic Anions in Drinking Water
-1.9
-1.5
AutoSuppression® recycle by Ion Chromatography
GPC-Purified and external water modes
10 µL of 40 µg/mL
Peaks: 1. Chloride AN 135: Determination of Inorganic Anions in Wastewater by
2. Sulfate
µS 1
2
3
3. Trifluoroacetate Ion Chromatography
AN 166: Application of Eluent Generation for Trace Anion

-1.9
0 4 8 12 16 20 Analysis of Borated Waters
Minutes 13032
AN 2: Determination of Nitrate and Sulfate Collected on Air
Determination of anionic counterions present in a gel permeation puri- Filters
fied peptide.
AN 2: Determination of Nitrate and Sulfate Collected on Air
Filters
Separation of the Common Anions and Acetate
Technical Notes
10 1 3 Column: IonPac AG14, AS14 4-mm TN 47: Achieving Low Baseline Noise for Anion Determination
Eluent: 3.5 mM Sodium carbonate
1 mM Sodium bicarbonate by Suppressed Conductivity Using Carbonate Eluents
4
5 6 Inj. Volume: 10 µL
µS
7
ASRS®-ULTRA 4-mm,
AutoSuppression® recycle mode Ordering Information
2. Acetate 20
3. Chloride 10
IC & RFIC Columns

0 4. Nitrite 15 Analytical Columns
5. Bromide 25
0 2 4 6 8 10 12 14
Minutes
6. Nitrate 25 IonPac AS14 Analytical Column (4 × 250 mm)............................................. 046124
7. Phosphate 40
8. Sulfate 30 IonPac AS14 Analytical Column (2 × 250 mm)............................................. 046129
12117-01
Guard Columns
Isocratic separation of inorganic anions on an IonPac AS14 column in
less than 13 minutes. IonPac AG14 Guard Column (4 × 50 mm)..................................................... 046134
Related Literature
Product Data Sheets
www.dionex.com 183
IonPac AS14A Analysis of a Wastewater Sample for Anions
Fast analysis of the common inorganic anions in Using the IonPac® AS14A Column
diverse sample matrices 8

Column: IonPac AG14A, AS14A-5µm
(3 × 150 mm)
2 4 Eluent: 8.0 mM Sodium carbonate
The IonPac AS14A anion-exchange column is a carbonate- Temperature: 30 °C
based column for fast analysis of the common inorganic anions Flow Rate: 0.5 mL/min
Inj. Volume: 5 µL
µS
in diverse sample matrices. The AS14A meets the performance Detection: Suppressed conductivity,
ASRS® ULTRA, 2 mm,
requirements specified in US EPA Method 300.0 (A). 2
3 5 6 AutoSuppression® recycle mode
7 Sample Prep: Diluted 1:100
1
0 Peaks: 1. Unknown
• High-efficiency and fast analysis (8 minutes) 2. Acetate
3. Formate
0 2 4 6 8 10 4. Chloride
• High-capacity applications (13 minute run time) Minutes 5. Bromide
6. Nitrate
7. Phosphate
• Improved peak shape, efficiency, and pH stability 8. Sulfate 15969
• Meets or exceeds US EPA Method 300.0 (A) performance

Determination of inorganic anions in a wastewater sample from a
requirements
chemical manufacturer on an IonPac AS14A-5µm (3 × 150 mm)
• Simplified operation with AS14A Eluent Concentrate and column.
Combined Seven Anion Standard
Analysis of Municipal Drinking Water on the IonPac® AS14A
The AS14A is designed for the separation of inorganic anions,
including fluoride, chloride, nitrite, bromide, nitrate, phosphate, 100 A
Column: IonPac AG14A,
2
AS14A-5µm (3 × 150 mm)
and sulfate, using a carbonate/bicarbonate eluent coupled with Eluent: 8.0 mM Sodium carbonate
suppressed conductivity detection. The inorganic anions can µS
7
Temperature: 30 °C
easily be determined in drinking water, wastewater, foods and 1 34 56 Flow Rate: 0.5 mL/min
beverages, scrubber solutions, and other diverse sample matri- Detection: Suppressed conductivity, ASRS®,
2 2 7 AutoSuppression® recycle mode
ces. B
4 Sample: Drinking water from Sunnyvale,CA
µS
IC & RFIC Columns

For simplified operation use the AS14A with AS14A Eluent 0
1 3 56 2. Chloride
3. Bromide
42.0
0.03
Concentrate and the Combined Seven Anion Standard. Use the 4. Nitrate 3.2
0 5 10 5. Chlorate 0.14
ASRS 300 suppressor with the AS14A column for low-noise Minutes 6. o-Phosphate 1.05
7. Sulfate 61.0
operation.
15724
Note: See also the IonPac AS22, the newest carbonate eluent Determination of inorganic anions in municipal drinking water on the
column recommended for fast analysis of inorganic anions. IonPac AS14A-5µm (3 × 150 mm) column.
184 www.dionex.com
IonPac AS14A
Comparison of the IonPac AS14A 3-mm and 4-mm Columns

for the Separation of the Inorganic Anions
12
A Analytical Columns
Column: A) IonPac® AG14A, AS14A-7µm (4 x 250 mm)
B) IonPac AG14A, AS14A-5µm (3 x 150 mm)
3 Eluent: 8.0 mM Sodium carbonate IonPac AS14A-7µm Analytical Column (4 × 250 mm).................................. 056904
µS 1 8
4 5 6 1.0 mM Sodium bicarbonate
2
7
Temperature: 30 °C IonPac AS14A-5µm Analytical Column (3 × 150 mm).................................. 056901
Flow rate: A) 1.0 mL/min
0 B) 0.5 mL/min
12 B 1 3
Inj. volume: A) 10 µL
B) 5 µL
Guard Columns
6 8 Detection: Suppressed conductivity, ASRS-ULTRA,
4 5 AutoSuppression® recycle mode IonPac AG14A-7µm Guard Column (4 × 50 mm).......................................... 056897
µS 7
2
Peaks: 1. Fluoride 5 mg/L (ppm) 5. Bromide 25 IonPac AG14A-5µm Guard Column (3 × 30 mm).......................................... 056899
2. Acetate 20 6. Nitrate 25
3. Chloride 10 7. Phosphate 40
0 4. Nitrite 15 8. Sulfate 30
0 5 10 15
Minutes
17158
Comparison of the separation of inorganic anions on 3 mm and 4 mm

IonPac AS14A columns.
Analysis of Toothpaste Using the IonPac® AS14A

A) Anion Standard Column: IonPac AG14A, AS14A-5µm
10 1 (3 × 150 mm)
3 Eluent: 8.0 mM Sodium carbonate
6 9 1.0 mM Sodium bicarbonate
5
µS 2 4 7 Temperature: 30 °C
Inj. Volume: 10 µL
10 9 A
B) Toothpaste Sample 8 2. Formate 10
1
3. Chloride 10
IC & RFIC Columns

µS 4. Nitrite 15
unk 7 5. Bromide 25
2 3 6. Nitrate 25
6
0 7. Phosphate 40
8. Monofluorophosphate 50
0 5 10 9. Sulfate 30
Minutes 15726
Determination of anionic additives in toothpaste on an IonPac AS14A-

5µm (3 × 150 mm) column.
Related Literature
Product Data Sheets
IonPac AS14A Anion-Exchange Column Data Sheet
Application Notes
AN 140: Fast Analysis of Anions in Drinking Water by Ion

Chromatography
www.dionex.com 185
IonPac AS12A Separation of Oxyhalides Using the IonPac® AS12A Column
Fast separation of inorganic anions, with excellent
Column: IonPac AG12A, AS12A 4-mm
retention of fluoride 6 2
The IonPac AS12A anion-exchange column provides fast analy- Inj. Volume: 10 µL
sis of common inorganic anions and oxyhalides, such as chlorite 1 ASRS-ULTRA 4-mm,
3 5 AutoSuppression recycle mode
and bromate, in environmental waters. This column is also use- µS 6
8 10 Peaks: 1. Fluoride 3.0 mg/L (ppm)
ful for trace chloride and sulfate in high-carbonate matrices. 7 9
2. Chlorite 20.0
3. Bromate 20.0
4. Chloride 5.0
• Provides fast analysis of inorganic anions and oxyhalides at 5.
6.
Nitrite
Bromide
10.0
20.0
0
similar concentrations 7. Chlorate 20.0
0 2 4 6 8 10 12 14 8. Nitrate 20.0
Minutes 9. Phosphate 30.0
• Resolves trace chloride and sulfate high-carbonate samples 10. Sulfate 20.0
10191
The AS12A is a moderate-capacity, carbonate eluent anion-
exchange column designed for the fast, isocratic separation of Isocratic separation of inorganic anions and oxyhalides on the IonPac
inorganic anions and oxyhalides, including fluoride, chlorite, AS12A column.
bromate, chloride, nitrite, bromide, chlorate, nitrate, phosphate,
and sulfate in drinking water, wastewater, groundwater, and
Determination of Anions in High Purity Solvent
other diverse sample matrices.
The common inorganic anions and oxyhalides can be deter- 3 Column: IonPac® AS12A and AG12A
mined in less than 12 min using an isocratic carbonate/bicarbon- 3
ate eluent coupled with suppressed conductivity detection. It µS
resolves chloride from high concentrations of carbonate. Use 1 AutoSuppression® recycle mode
Sample: 4.5 mL Electronics grade
the Anion Self-Regenerating Suppressor (ASRS 300) with the 4 Isopropanol concentrated on TAC-LP1
0 1.1 mL DI water wash
AS12A column.
Peaks: 1. Chloride 4 µg/L (ppb)
IC & RFIC Columns
2. Unidentified —
3. Unidentified —
Note: For trace bromate in ozonated drinking water, use the 0 2 4 6 8 10
Minutes
12 14 16
4. Sulfate 5
high-capacity IonPac AS23, recommended for determination of

oxyhalides and inorganic anions.
10198
Isocratic separation of anions in high-purity solvents using the IonPac

AS12A column.
186 www.dionex.com
IonPac AS12A
Borate Gradient Separation of Anions Ordering Information

Column: IonPac® AG12A, AS12A, 4 mm
Eluent: Gradient from 11mM borate/
8.25 mM hydroxide to 26.5 mM Analytical Columns
10 borate/27.5 mM hydroxide in 16 min
4 13 Flow Rate: 1.5 mL/min
Inj. Volume: 10 µL IonPac AS12A Analytical Column (4 × 200 mm).......................................... 046034
1 3 Detection: Suppressed conductivity, ASRS®,
6 9 chemical suppression mode IonPac AS12A Analytical Column (2 × 200 mm).......................................... 046055
10 12 Peaks: 1. Fluoride 5.0 mg/L (ppm)
µS 2 5 8 2. Acetate 10.0
11
3. Formate 5.0 Guard Columns
4. Chlorite 5.0
5. Bromate 5.0
7 6. Chloride 1.0 IonPac AG12A Guard Column (4 × 50 mm)................................................... 046035
7. Carbonate —
0 8. Nitrite 2.0 IonPac AG12A Guard Column (2 × 50 mm)................................................... 046056
9. Bromide 5.0
0 5 10 15 20 10. Chlorate 5.0
Minutes 11. Nitrate 5.0
12. Phosphate 10.0
13. Sulfate 5.0
10197
Borate gradient separation of anions using the IonPac AS12A column.
Determination of Anionic Impurities in Caustic Soda

Using the IonPac® AS12A Column
2
1 Column: IonPac AG12A, AS12A, 4 mm
Eluent: 2.7 mM Sodium carbonate,
µS Inj. Volume: 100 µL
Sample prep: Diluted 1:20, neutralized with ASRN
2 3 Detection: Suppressed conductivity, ASRS®,
AutoSuppression®
0 external water mode
Peaks: 1. Chloride 13.2 mg/L (ppm)
2. Chlorate 21.4
IC & RFIC Columns

0 3 6 9 12 3. Sulfate 11.1
Minutes
14934
Determination of anionic impurities in caustic soda using the IonPac

AS12A column.
Related Literature
Product Data Sheets
IonPac AS12A Anion-Exchange Column Data Sheet
www.dionex.com 187
IonPac AS9-HC Separation of Inorganic Anions
High-capacity column for the analysis of inorganic and Oxyhalides Using the AS9-HC Column
anions and oxyhalides including bromate. 8
Column: IonPac AG9-HC, AS9-HC 4 mm

Specifically designed for trace bromate in drinking water, the 4 5
Eluent:
Inj. Volume:
9.0 mM Sodium carbonate
25 µL
1 2
AS9-HC is the specified column for U.S EPA Methods 300.1 3
6 8
10 Detection: Suppressed conductivity, ASRS-II,
AutoSuppression, recycle mode
and 317.0. µS 7 Peaks: 1.Fluoride 3 mg/L
9
2.Chlorite 10
3.Bromate 20
• For the analysis of oxyhalides and inorganic anions in com- 4.Chloride 6
5.Nitrite 15
plex sample matrices 0 6.Bromide 25
7.Chlorate 25
8.Nitrate 25
• Simple, isocratic method for trace bromate (5 µg/L) in 0 5 10 15 20 25
9.Phosphate 40
Minutes
ozonated drinking water matrices 10.Sulfate 30
12966
• Ideal for difficult applications, such as trace nitrite in com-
plex sample matrices Separation of inorganic anions and oxyhalides using the AS9-HC
column.
• Improved separation of bromate/chloride, chloride/nitrite,
chlorate/nitrate analyte pairs
Determination of Trace Levels of Oxyhalides and Bromide
The AS9-HC is a high-capacity carbonate eluent anion-ex- in Simulated Drinking Water Using the AS9-HC Column
change column with selectivity similar to the AS9-SC column. 0.2 1 4 5 8 10 Column: IonPac AG9-HC, AS9-HC
A. Eluent: 9.0 mM Sodium bicarbonate/
The AS9-HC also supports the analysis of oxyhalides and Simulated Flow Rate: 1.0 mL/min
inorganic anions, including fluoride, chlorite, bromate, chloride, µS
9
Drinking
Water
Injection:
Detection:
200 µL
Suppressed conductivity
nitrite, bromide, chlorate, nitrate, phosphate, and sulfate. The 2
3 67 ASRS-II, AutoSuppression®
recycle mode
AS9-HC is specified in validated methods such as US EPA 0.0
Peaks: A B
Method 300.1 and 317.0 and meets or exceeds the performance 0.5 1 4 8 10
1. Fluoride
2. Chlorite
1.0
0.01
0.04 mg/L (ppm)
requirements of these methods. B. 3. Bromate 0.005

Sunnyvale 4. Chloride 50.0 16.2
IC & RFIC Columns
µS Drinking 5. Nitrite 0.1

Water 6. Bromide 0.01 0.03
This column separates trace bromate in drinking water ma- 67 9 7. Chlorate 0.01 0.04
0.0 8. Nitrate 10.0 3.9
trices using an isocratic carbonate eluent and a large-loop 9. Phosphate 0.1 0.15
0 5 10 15 20 25
injection. The AS9-HC also offers good retention of fluoride Minutes
10. Sulfate 50 18.3
12967-01
out of the water dip. The column’s high capacity (190 µeq for
4 × 250 mm) increases retention time to approximately 22 min. Determination of trace bromate in drinking water using the AS9-HC
Use the Anion Self-Regenerating Suppressor (ASRS 300) with column.
the AS9-HC column.
Note: The AS23 is the newest carbonate eluent column

recommended for the analysis of oxyhalides including bromate.
188 www.dionex.com
IonPac AS9-HC
Analysis of Wastewater for Anions Using the IonPac® AS9-HC Column AN 149: Determination of Chlorite, Bromate, Bromide, and
3 3 8 Chlorate in Drinking Water by Ion Chromatography with an
A
Column: IonPac AG9-HC, AS9-HC, 4-mm
On-Line-Generated Postcolumn Reagent for Sub-µg/L Bromate
1 7 Eluent: 9.0 mM Sodium carbonate Analysis
Inj. Volume: 25 µL
2 4
5 6 Detection A: Suppressed conductivity, ASRS®, AN 81: Ion Chromatographic Determination of Oxyhalides and
0 Detection B: UV at 214 nm Bromide at Trace Level Concentrations in Drinking Water Us-
A B ing Direct Injection
0.04 Peaks: 1. Fluoride 1.14 — mg/L (ppm)
B
2. Acetate — —
3. Chloride — — AN 85: Determination of Trace Anions in Organic Solvents
AU 4. Nitrite 0.14 0.18
6 5. Bromide 0.82 0.79
5
4 6. Nitrate
7. Phosphate
1.47
21.1
1.58
—
Technical Notes
0
8. Sulfate 30.3 —
Sample: Synthetic wastewater containing TN 45: Determination of Trace Anions in Concentrated Hydro-
2000 mg/L chloride
0 5 10 15 20 25 fluoric Acid
14928
Minutes

Analysis of wastewater for anions using the IonPac AS9-HC column.
colic Acid
Analysis of Spiked Tap Water Using EPA Method 317.0

Column: IonPac® AG9-HC, AS9-HC, 4 mm
1.20
A Flow Rate: 1.3 mL/min
Inj. Volume:
Detector:
225 µL
A) Suppressed conductivity,
Analytical Columns
µS
34 AutoSuppression® external water IonPac AS9-HC Analytical Column (4 × 250 mm)......................................... 051786
2 B) Absorbance, 450 nm
0
Postcolumn IonPac AS9-HC Analytical Column (2 × 250 mm)......................................... 052244
Reagent: o-Dianisidine
6.0 × 10-3 PCR Flow Rate: 0.7 mL/min
B
Postcolumn
Heater: 60 °C Guard Columns
AU
Peaks: A
5 1. Chlorite 109.4 µg/L (ppb) IonPac AG9-HC Guard Column (4 × 50 mm)................................................. 051791
IC & RFIC Columns

0 2. Bromate 11.92
3. Bromide 135.0 IonPac AG9-HC Guard Column (2 × 50 mm)................................................. 052248
4. Chlorate 168.8
0 5 10 15 20 25
Minutes B
5. Bromate-UV 11.54 15696
Analysis of spiked tap water using EPA Method 317.0.
Related Literature
Product Data Sheets
IonPac AS9-SC and AS9-HC Anion-Exchange Columns Data

Sheet
Application Notes
AN 135: Determination of Inorganic Anions in Wastewater by

Ion Chromatography
AN 136: Determination of Inorganic Oxyhalide Disinfection

Byproduct Anions and Bromide in Drinking Water Using Ion
Chromatography with the Addition of a Postcolumn Reagent for
Trace Bromate Analysis
www.dionex.com 189
IonPac AS9-SC Separation of Inorganic Anions and Oxyhalides
For the fast isocratic separation of inorganic anions Using the AS9-SC Column
and oxyhalides 15 Column: IonPac® AG9-SC, AS9-SC, 4 × 250 mm
10 1.7 mM Sodium bicarbonate
The AS9-SC is designed for the isocratic separation of oxyha- 7
5 6
lides and inorganic anions, including fluoride, chlorite, bromate, 1
µS 4 9 ASRS® ULTRA 4-mm,
chloride, nitrite, bromide, chlorate, nitrate, phosphate, and AutoSuppression®, recycle mode
3
sulfate in drinking water, wastewater, groundwater, and other Peaks: 1. Fluoride 1 mg/L (ppm)
2. Chlorite 5
diverse sample matrices. 3. Bromate 5
4. Chloride 1.5
0 5. Nitrite 6
• For fast analysis of oxyhalides and inorganic anions at 6. Bromide 10
7. Chlorate 15
similar concentrations 0 1 2 3 4 5 6 7 8 9 8. Nitrate 15
Minutes 9. Phosphate 20
10. Sulfate 25
• Ideal for simple sample matrices 12966A
Common inorganic anions and oxyhalides can be determined Separation of inorganic inions and oxyhaldies using the AS9-SC
in less than 10 minutes using an isocratic carbonate/bicarbonate column.
eluent coupled with suppressed conductivity detection.
Determination of Oxyhalides and Bromide
The AS9-SC column is specified in US EPA Method 300.0 (B),
and meets or exceeds the performance requirements of
1 4 8 10
this method. Use the Anion Self-Regenerating Suppressor Peaks:
0.4
(ASRS 300) with the AS9-SC column. 5
1. Fluoride
2. Chlorite
1.0 mg/L
0.02
3. Bromate 0.005
4. Chloride 50.0
Note: See also the AS23, the recommended column for the µS
5. Nitrite
6. Bromide
0.1
0.02
2 9
analysis of oxyhalides, including bromate. 6 7 7. Chlorate 0.02
3 8. Nitrate 10.0
9. o-Phosphate 0.1
10. Sulfate 50.0
IC & RFIC Columns
–0.1 Sample also contained

0 5 10 15 20 25 150 mg/L Bicarbonate
Minutes
13270
Determination of oxyhalides and bromide in simulated drinking water.
190 www.dionex.com
IonPac AS9-SC
Application Updates
Trace Bromate Determination Using Preconcentration
AU 131: Determination of Nitrite and Nitrate in Drinking Water
0.15 1 Column: IonPac® AG10(concentrator) Using Chemically Suppressed Ion Chromatography
IonPac AS9-SC(analytical)
Eluent: 40 mM Boric acid
Bromate
µS
1 µg/L
20 mM Sodium hydroxide AU 132: Determination of Nitrite and Nitrate in Drinking Water
Purge Eluent: 200 mM Boric acid
100 mM Sodium hydroxide Using Ion Chromatography with Direct UV Detection
0.00
Eluent Flow Rate: 2 mL/min
Preconcentration
Flow Rate: 2 mL/min
Bromate 1
0.15
5 µg/L
Detection: Conductivity with ASRS®,
external water mode
µS Inj. Volume: 3.5 mL
Peak: 1. Chloride 200 mg/L
0.00 Sample pretreated with OnGuard™ Analytical Columns
(-Ba, -Ag, & -H) cartridges.
0 5 Sample also contained 200 mg/L Carbonate
Minutes
and 200 mg/L Sulfate. IonPac AS9-SC Analytical Column (4 × 250 mm)......................................... 043185
11291-01
Guard Columns
Trace bromate determination using preconcentration.
IonPac AG9-SC Guard Column (4 × 50 mm).................................................. 043186
Combustion—IC of Polymeric Catalyst
1
60
Column: IonPac® AS9-SC, AG9-SC
Eluent: 3 mM sodium carbonate
Flow rate: 2 mL/min
Inj. volume: 50 µL
µS Peaks: 1. Chloride
2. Nitrate
3. Sulfate
3 Concentrations (n=3):
Chlorine, 10.9% ± 0.01%
Sulfur, 4.9% ± 0.01%
IC & RFIC Columns

2
0
0 2 4 6 8
Minutes
12975-01
Determination of chloride and sulfate in a polymeric catalyst using the

AS9-SC column.
Related Literature
Product Data Sheets
IonPac AS9-SC and AS9-HC Anion-Exchange Columns Data

Sheet
Application Notes
AN 101: Trace Level Determination of Bromate in Ozonated

Drinking Water Using Ion Chromatography
AN 51: Method for Determination of Anions in Sodium Hy-

droxide
www.dionex.com 191
IonPac AS4A-SC Determination of Anions in Drinking Water
For the fast isocratic separation of inorganic anions Using the IonPac® AS4A-SC Column
in drinking water and wastewater Column: IonPac AG4A-SC, AS4A-SC, 4 mm
35
2 Eluent: 1.7 mM Sodium bicarbonate/
1.8 mM Sodium carbonate
The IonPac AS4A-SC 2 mm and 4 mm anion-exchange col- Flow Rate: 2.0 mL/min
umns are designed for the fast analysis of inorganic anions in Injection: 50 µL
environmental waters. The AS4A-SC was introduced in 1984 µS
ASRS® ULTRA, 4 mm,
and has been the preferred column for inorganic anion analysis Peaks: 1. Fluoride 0.03 mg/L (ppm)
for many years. It is the specified column in US EPA Method 1 4 5
2. Chloride 9.30
3 3. Nitrate 2.51
300.0 (A). 4. Phosphate 0.10
5. Sulfate 10.9
0
0 2 4 6 8 10 Sample: Drinking water from Sunnyvale, CA
• Resolves inorganic anions in 8 min using an isocratic car- Minutes
bonate/bicarbonate eluent.
14579
• Sodium tetraborate gradient optimizes difficult separations.

Determination of inorganic anions in a drinking water sample using an
• Meets or exceeds US EPA Method 300.0 (A) performance IonPac AS4A-SC column.
requirements.
• Provides excellent performance for fast analysis of inor- Determination of Inorganic Anions in Wastewater
ganic anions. Using the IonPac® AS4A-SC Column
The IonPac AS4A-SC (solvent-compatible) is a low-capacity 20

Column:
Eluent:
IonPac AG4A-SC, AS4A-SC, 4 mm
1.7 mM Sodium bicarbonate/
4
carbonate eluent anion-exchange column for the fast, isocratic 8 1.8 mM Sodium carbonate
separation of inorganic anions such as nitrate or sulfate, us- Injection: 50 µL
ing a carbonate/bicarbonate eluent coupled with suppressed 3
ASRS® ULTRA, 4 mm,
µS
conductivity detection. Inorganic anions are easily determined 2
5 6 Peaks: 1. Unknown —
in any liquid, including water, foods, and other diverse sample 1 2. Fluoride 2.1 mg/L (ppm)
0 3. Carbonate —
IC & RFIC Columns
matrices. 4. Chloride 49
0 2 4 6 8 10 5. Bromide .037
Minutes 6. Nitrate .034
Separation of the common inorganic anions can be achieved in 7. Phosphate
8. Sulfate
28
35
less than 10 min using an isocratic carbonate/bicarbonate eluent.
14254
For simplified operation, use the AS4A-SC Eluent Concentrate
and the Combined Seven Anion Standard. The Anion Self- Determination of inorganic anions in a wastewater sample using an
Regenerating Suppressor (ASRS 300) is recommended for use IonPac AS4A-SC column.
with the AS4A-SC column.
Note: The AS22 is the newest carbonate eluent column

recommended for fast analysis of inorganic anions.
192 www.dionex.com
IonPac AS4A-SC
Tetraborate Gradient Separation of Anions
Using the IonPac® AS4A-SC Column
Analytical Columns
Column: IonPac AS4A-SC, 2-mm
30
Eluent: 1. DI H2O
IonPac AS4A-SC Analytical Column (2 × 250 mm)....................................... 043125
3
9 2. 50 mM Sodium tetraborate
1
4 Gradient: 5 mM Na2B4O7 to 28 mM IonPac AS4A-SC Analytical Column (4 × 250 mm)....................................... 043174
Na2B4O7 in 10 min
µS Sample Loop: 15 µL
2 8
7 Detector: Suppressed Conductivity Guard Columns
5
2. Acetate 18
3. Formate 18 IonPac AG4A-SC Guard Column (4 × 50 mm)............................................... 043175
0 4. Chloride 10
5. Nitrite 5 IonPac AG4A-SC Guard Column (2 × 50 mm)............................................... 043126
0 5 10 15
6. Bromide 10
Minutes
7. Nitrate 20
8. Phosphate 20
9. Sulfate 20
8236
Tetraborate gradient separation of anions using the IonPac AS4A-SC

2 mm column.
Trace Anions in Solvents Using the IonPac® AS4A Column
2
1
Column: IonPac AS4A-SC 4-mm

µS Eluent: 1.7 mM Sodium bicarbonate /
4 1.8 mM Sodium carbonate
2
0 Sample Loop: 250 µL
Sample: 50-ppb anions in 80% isopropanol
Peaks: 1. Chloride
2. Nitrate
IC & RFIC Columns

3. Phosphate
4. Sulfate
–2
0 2 4 6 8
Minutes
8238
Direct injection of anions in a solvent extract using the IonPac AS4A-

SC column.
Related Literature
Product Data Sheets
IonPac AS4A-SC Anion-Exchange Column Data Sheet
Application Notes
AN 133: Determination of Inorganic Anions in Drinking Water

by Ion Chromatography
AN 135: Determination of Inorganic Anions in Wastewater by

Ion Chromatography
AN 31: Determination of Anions in Acid Rain by Ion Chroma-

tography
AN 36: Determination of Oxalate in Urine by Ion Chromatog-

raphy
www.dionex.com 193
IC & RFIC Columns
194 www.dionex.com
Specialty Anion-Exchange Columns
Anion-exchange columns for special applications
These anion-exchange columns support special applications,
such as non-suppressible eluents, in combination with a variety
of detection modes, including amperometric and UV-VIS detec-
tion.
IonPac AS7: High-capacity, high-efficiency, hydrophobic anion-

exchange column designed for the separation of a wide range of
polyvalent anions.
IonPac AS5: Low-capacity, hydroxide-selective anion-exchange
column for separating higher-valence anions
IC & RFIC Columns
www.dionex.com 195
IonPac AS7 Separation of Polyphosphonate Scale Inhibitors
For the separation of a wide range of polyvalent Using the IonPac® AS7 Column
anions
Column: IonPac AS7 4-mm
.008 Eluent: 30 mN Nitric acid
The IonPac AS7 separates a wide variety of polyvalent anions, 2
including polyphosphates, polyphosphonates, and other poly- 4
5
Postcolumn
3 Reagent: 1 g/L Fe(NO3)3 • 9 H2O
valent complexing agents such as EDTA and NTA using acidic AU 2% (v/v) HClO4
0.8 mL/min
elution (eliminating metal interferences) with post-column Inj. Volume: 25 µL
1 Detection: UV, 330 nm
derivatization and UV-Vis detection. 0
Peaks: 1. Phosphate -
2. Dequest® 2010 50 mg/L (ppm)
3. Dequest 2051 50
• For polyvalent anions and chelating agents in complex 0 2 4 6 8 10
4. Dequest 2000 50
Minutes
sample matrices 5. Dequest 2041 50
• Determines cyanide and sulfide using amperometric detec- 11784

tion
Determination of polyphosphonate scale inhibitors using the AS7 4 mm
• Useful for hexavalent chromium in environmental matrices column.
The AS7 column has a unique polymer packing that provides

Determination of Chelating Agents Using the AS7 Column with
superior performance for separating ionic and polar compounds. Postcolumn Addition of Ferric Nitrate Coupled with UV Detection
The patented packing offers high-speed, high-efficiency, and
high-loading capacity at moderate backpressures.
.006 Column: IonPac® AG7, AS7, 4 mm
Eluent: 24 mM Nitric acid
Note: The newer IonPac AS16 or AS20 column is 1
3
Flow Rate:
Postcolumn Reagent:
0.5 mL/min
1 g/L Fe (NO3)3 • 9 H2O
2
recommended for polyphosphates. µS
2% (v/v) HCIO4
0.5 mL/min
Inj. Volume: 25 µL
0 Peaks: 1. Phosphate 100 mg/L (ppm)

IC & RFIC Columns
2. NTA 20
3. EDTA 10
0 2 4 6 8 10
Minutes
11785
Chelating agents by UV detection with postcolumn ferric nitrate.
196 www.dionex.com
IonPac AS7
Application Notes
Determination of Cyanide and Sulfide
AN 80: Determination of Dissolved Hexavalent Chromium in
Drinking Water, Groundwater and Industrial Waste Water Ef-
fluents by Ion Chromatography
Column: IonPac AS7, 2 mm
Eluent: 75 mM NaOH/100 mM NaAc
1
Flow Rate: 0.25 mL/min Application Updates
nC Inj. Volume: 10 µL
50 ppm CI–
Detection: ED40 with integrated pulsed
2 amperometric detection (IPAD)
2–
AU 107: Determination of Cyanide in Strongly Alkaline Solu-
Peaks: 1. S 50 µg/L (ppb)
2. CN 50
– tions
Sample: 50 mg/L Chloride
AU 144: Determination of Hexavalent Chromium in Drinking
0 5 10 15 20
Minutes Water Using Ion Chromatography
Technical Notes
23027
TN 26: Determination of Cr(VI) in Water, Waste Water, and

Determination of cyanide and sulfide using the AS7 2 mm column with Solid Waste Extracts
amperometric detection and a disposable silver electrode.
EPA Method 218.6: Chromium in Waste Water
10 ppb 50 ppb 100 ppb

Analytical Columns
Column: IonPac® AS7
Eluent: 250 mM ammonium sulfate
100 mM ammonium hydroxide IonPac AS7 Analytical Column (4 × 250 mm)............................................... 035393
Post-Column IonPac AS7 Analytical Column (2 × 250 mm)............................................... 063097
Reagent: 2 mM diphenylcarbazide
10% methanol
1N sulfuric acid
Post-Column
Guard Columns
Detector: Absorbance, 530 nm IonPac AG7 Guard Column (4 × 50 mm)....................................................... 035394
IC & RFIC Columns

IonPac AG7 Guard Column (2 × 50 mm)....................................................... 063099
0 2 4 0 2 4 0 2 4
Minutes
5747-02
Determination of hexavalent chromium using the IonPac AS7 column.
Related Literature
Product Data Sheets
IonPac AS7 Anion Exchange Column Data Sheet
www.dionex.com 197
IonPac AS5 Separation of Cyano-Transition Metal Complexes
For separating higher-valence anions Eluent A: 20 mM NaOH, 150 mM NaCN
5 Eluent B: 20 mM NaOH, 300 mM NaCIO4
Eluent C: 20 mM NaOH
The IonPac AS5 separates higher-valence anions, including
Gradient: t %A %B %C
polyphosphates, oxyanions, EDTA complexes, metal cyanide 0.0 10% 10% 80%
complexes, and hydrophobic anions such as iodide, thiosulfate, 3 0.5 10 10 80
18.0 10 45 45
and thiocyanate.
Inj. Volume: 50 µL
• For metal-EDTA complexes 7
8
Detection: UV-Vis, 215 nm
Peaks: 1. Ag 10 mg/L (ppm)
4 6
1 2. Au 10
• For cyano-transition metal complexes 2
3. Cu 10
4. Ni 10
• For hydrophobic anions, including iodide, thiocyanate, and 0 5 10 15 20 25
5. Fe (II)
6. Pd (II)
10
10
thiosulfate Minutes 7. Cu (III) 10
8. Pt (II) 10 3462
• AS16 or AS20 columns are recommended for hydrophobic

anions and highly charged anions Separation of cyano-transition metal complexes.
The AS5’s compatibility with weak eluents simplifies the de-

termination of strongly-retained species, since these eluents are Determination of Anions in an Electroless Nickel Plating Bath
compatible with a suppressor. Furthermore, the rapid elution of A
4
6
stronly-retained species greatly improves peak shapes, lowers

minimum detection limits, and increases sample throughput Column: IonPac® AG5, AS5 4-mm
Eluent: 2.8 mM Sodium carbonate /
potential. 2 2.2 mM Sodium bicarbonate
Peaks: A B
The selectivity of the AS5 makes it possible to rapidly elute 1. Fluoride 3 mg/L (ppm)
strongly-retained species such a iodide and thiocyanate in brines 6
2. Hypophosphite 30 39
3. Chloride 5
without interference from the large chloride peak typical of such B 4
4. Phosphite 30 65
5. Nitrate 30
IC & RFIC Columns
5
samples. 2 7 6. Sulfate 30 46
3 8 7. Phosphate 30
1
8. Citrate 20
Note: IonPac AS16 or AS20 columns are recommended for
0 5 10 15 20
hydrophobic anions and highly-charged anions. Minutes
3647
Determination of anions in an electroless nickel plating bath.
198 www.dionex.com
IonPac AS5
Separation of EDTA from Nickel-EDTA Complexes

2 Analytical Columns
Column: IonPac AG5, AS5 4-mm
Eluent: 2.8 mM Sodium carbonate, IonPac AS5 Analytical Column (4 × 250 mm)............................................... 035395
Detection: Suppressed conductivity Guard Columns
Peaks: 1. Ni-EDTA 10 mg/L (ppm)
1 2. EDTA 50
IonPac AG5 Guard Column (4 × 50 mm)....................................................... 035396
0 2 4 6
Minutes 4145
Separation of EDTA from nickel-EDTA complexes using the IonPac

AS5 column.
Isocratic Separation of Strongly Retained Anions

2
Column: IonPac AS5 4-mm
1 3 Eluent: 4.5 mM Sodium Carbonate /
2.0 mM Sodium Hydroxide /
0.8 mM 4-Cyanophenol /
2% Acetonitrile
Peaks: 1. Iodide
IC & RFIC Columns

2. Thiocyanate
3. Thiosulfate
0 4 8 12 16
Minutes
7652
Isocratic separataion of strongly-retained anions using the IonPac AS5

column.
Related Literature
Product Data Sheets
IonPac AS5 Data Sheet
Application Notes
AN 51: Method for Determination of Anions in Sodium Hy-

droxide
AN 55: Determination of Metal Cyanides
www.dionex.com 199
IC & RFIC Columns
200 www.dionex.com
Cation-Exchange Colums
A complete family of columns for separation of
inorganic cations, ammonium, and amines
Dionex cation-exchange columns provide high-resolution
separations of inorganic cations, ammonium, and amines. They
provide an excellent approach to separations of alkali metals,
alkaline earth metals, alkylamines, alkanolamines and biogenic
amines. The carboxylate-functionalized cation-exchange col-
umns are available in a wide range of capacities and hydropho-
bicities. They are hydronium-selective and are compatible with IonPac CS18: Low-capacity carboxylate-functionalized cation-
RFIC-EG and RFIC-ER systems. exchange column for the separation of polar amines such as
ethanolamines.
IonPac CS17: Low-capacity carboxylate-functionalized cation-
exchange column for gradient profiling and amine determina-
tion.
IonPac CS16: High-capacity carboxylate-functionalized cation-
exchange column for disparate concentration ratios of adjacent
cations such as sodium and ammonium.
IonPac CS15: Medium-capacity carboxylate-functionalized
cation-exchange column with unique selectivity for disparate
concentration ratios of sodium and ammonium.
IonPac CS14: Medium low-capacity carboxylate-functionalized
cation exchange column for aliphatic and aromatic amines and
IC & RFIC Columns

polyamines.
IonPac CS12A: Medium-capacity carboxylate-functionalized
column for fast, isocratic separation of inorganic cations.
IonPac CS11: Sulfonate-functionalized cation-exchange column
for isocratic separations using HCl and diaminopropionic acid
eluents.
IonPac CS10: Sulfonate-functionalized cation-exchange column
for isocratic separations using HCl and diaminopropionic acid
eluents.
www.dionex.com 201
IonPac CS18 Gradient Separation of Alkanolamines, Methylamines, and the
Common Inorganic Cations on the IonPac® CS18 Column
Low-capacity cation-exchange column for the 1.20
11 12
separation of polar amines
µS
1 2 3 4 5 7 8 910 Column: IonPac CS18, 2 mm
The IonPac CS18 carboxylate-functionalized cation-exchange 6 Eluent: 0.5 mM MSA,
–0.05 gradient to 1 mM at 20 min,
column is tailored for the separation of polar amines including 0 5 10 15 20 25 30 35 gradient to 6 mM at 22.4 min,
Minutes gradient to 8 mM at 28 min,
alkanolamines and methylamines, and moderately hydrophobic Peaks: 1. Lithium 0.1 mg/L (ppm) back to 0.5 mM at 28.1 min
and polyvalent amines including biogenic amines and alkyl 2. Sodium 0.4 Eluent Source: EGC II MSA
3. Ammonium 0.5 Flow Rate: 0.3 mL/min
diamines, using simple aqueous eluents and elevated tempera- 4. Ethanolamine 1.0 Temperature: 35 °C
5. Methylamine 1.0 Inj. Volume: 5 µL
ture. The CS18 is ideally used with RFIC systems for automatic 6. Diethanolamine 2.0 Detection: Suppressed conductivity,
CSRS® ULTRA II 2 mm,
methanesulfonic acid eluent generation. 7.
8.
Potassium
Dimethylamine
1.0
0.8 AutoSuppression® recycle mode
9. Triethanolamine 9.0
10. Trimethylamine 3.0
• For amines including alkanolamines and methylamines in 11. Magnesium 0.5
12. Calcium 1.0 20289
diverse sample matrices
• For moderately-hydrophobic amines incl. biogenic amines, The CS18 column can separate small, hydrophilic amines, including
alkanolamines and methylamines, in a single run using a modest acidic
alkyl diamines, and polyvalent amines gradient.
• Optimized for simple acidic gradient separations with
minimal baseline shift Biogenic Amines, Methylamines, and the
Common Six Cations on the IonPac® CS18 Column
• Ideal Reagent-Free electrolytic suppression with the Cation 0.90
14
Self-Regenerating Suppressor CSRS 300
Column: IonPac CS18, 2 mm
• Compatible with moderate amounts of organic solvents, µS 9 10 11
Eluent: 3 mM MSA, isocratic to 5 min,
23 5 8 13
excluding alcohols 1 4 67
12 15 gradient to 18 mM at 20 min,
gradient to 45 mM at 25 min,
16
–0.05 isocratic to 35 min,
• Requires only a modest acid concentration to elute polyva- 0 5 10 15 20 25 30 35
back to 3 mM at 35.1 min
Minutes Eluent Source: EGC II MSA
lent cations Peaks [mg/L (ppm)]:
IC & RFIC Columns
Temperature: 40 °C
1. Lithium 0.02 9. Calcium 0.2
Inj. Volume: 5 µL
2. Sodium 0.08 10. Putrescine 1.1
The IonPac CS18 cation-exchange column supports isocratic 3. Ammonium 0.10 11. Cadaverine 1.1
and gradient separations of polar amines, moderately hy- 4. Methylamine 0.25
5. Potassium 0.20
12.
13.
Histamine
Agmatine
1.6
1.4
drophobic amines, and polyvalent cations using suppressed 6. Dimethylamine 0.20 14. Spermidine 4.0
7. Trimethylamine 0.75 15. Spermine 0.4
conductivity detection. The CS18 column with nonsuppressed 8. Magnesium 0.1 16. Phenethylamine 1.5 22798
conductivity detection is recommended when extended calibra-
tion linearity for ammonium or weak bases is required. The The IonPac CS18 column can easily separate biogenic amines, meth-
CS18 can be used for many of the nonsuppressed applications ylamines, and Group I and II cations using an aqueous eluent without
organic solvent.
supported by the IonPac SCS-1 column but with much greater
column stability.
The IonPac CS18 is targeted for analysis of power plant waters

treated with ammonium, morpholine, or ethanolamine; chemi-
cal additives; chemical process solutions; scrubber solutions;
personal care products; and food samples.
Note: Use the Cation Self-Regenerating Suppressor (CSRS

300) with the CS18 column.
202 www.dionex.com
IonPac CS18
Amines of Interest in the Petrochemical Industry on IonPac® CS18 Application Updates
Eluent: 0.5 mM MSA, gradient to 1 mM
at 20 min, gradient to 4 mM at 28 min,
Peaks: AU 162: Determination of Biogenic Amines in Fruit, Vege-
1. Lithium 0.05 mg/L (ppm)
gradient to 11 mM at 34 min,isocratic 2. Sodium 0.2 tables, and Chocolate Using Ion Chromatography with Sup-
to 40 min, back to 0.5 mM at 40.1 min 3. Ammonium 0.25
Eluent Source: EGC II MSA 4. Ethanolamine 3.0 pressed Conductivity and Integrated Pulsed Amperometric
Flow Rate: 0.30 mL/min 5. Methylamine 3.6
Temperature: 50 °C 6. Diethanolamine 3.6
Inj. Volume: 5 µL 7. Potassium 0.5
8. Ethylamine
9. Dimethylamine
3.0
1.4
1.00 AutoSuppression® recycle mode 10. N-methyl diethanolamine 3.0
5 16 11. Morpholine 3.2
12. 1-dimethylamino-2-propanol 3.7
8 15
13. N-methyl morpholine 7.5 Analytical Columns
4 12 14. Butylamine 1.5
µS 9
6+7
11
17 15. Magnesium 0.25 IonPac CS18 Analytical Column ( 2 × 250 mm)............................................ 062878
18 16. Calcium 0.5
3 10 13
1 2 17. Strontium 0.5
14
18. Barium 0.5
–0.05 Guard Columns
0 5 10 15 20 25 30 35 40
Minutes 22808
IonPac CG18 Guard Column (2 × 50 mm)...................................................... 062880
Separation of a variety of amines and the extended Group I and II inor-
ganic cations that are monitored in the petrochemical industry.
Power Amines and the Six Common Cations on the IonPac® CS18
Eluent: 0.5 mM MSA, gradient to 3 mM at 16 min,
gradient to 25 mM at 30 min,
Peaks:
back to 0.5 mM at 30.1 min
1. Lithium 0.05 mg/L (ppm)
Eluent Source: EGC II MSA
2. Sodium 0.20
3. Ammonium 0.25
Temperature: 40 °C
4. Ethanolamine 2.0
Inj. Volume: 5 µL
5. Potassium 0.50
Detection: Suppressed conductivity, CSRS® ULTRA II
6. 2(2-aminoethoxy)ethanol 3.0
2 mm, AutoSuppression® recycle mode
7. 5-amino-1-pentanol 3.0
1.20 8. Morpholine 3.0
13 14
9. 3-methoxypropylamine 3.0
10. 2-diethylaminoethanol 3.0
12
IC & RFIC Columns

11. 3-quinuclidinol 2.0
4 12. Magnesium 0.25
µS 8
5 13. Calcium 0.50
9 10 14. Ethylenediamine 2.0
3 6 7
1 2 11 15 15. Cyclohexylamine 3.0
–0.10
0 5 10 15 20 25 30
Minutes 22911
Separation of a variety of amines used in the power industry with the

IonPac CS18 column.
Related Literature
Product Data Sheets
IonPac CS18 Datasheet
Application Notes
AN 182: Determination of Biogenic Amines in Alcoholic Bev-

erages by Ion Chromatography with Suppressed Conductivity
and Integrated Pulsed Amperometric Detections
AN 183: Determination of Biogenic Amines in Fermented

and Non-Fermented Foods Using Ion Chromatography with
Suppressed Conductivity and Integrated Pulsed Amperometric
Detection
www.dionex.com 203
IonPac CS17 Gradient Separation of Diamines Using the IonPac® CS17
1.10
Low-capacity cation-exchange column for gradient A 8
9
11
Column:
Inj. Volume:
IonPac CS17, CG17, 2 mm
25 µL
3 Eluent: 3 mM MSA isocratic to 3.5 min;
profiling and amine determination µS 2
4 6 13 15
16
3 mM to 6 mM MSA gradient at 12 min; isocratic
1 5 to 15 min; gradient to 40 mM MSA at 20 min,
0.10 isocratic to 24 min; step change back to 3 mM
The IonPac CS17 carboxylate-functionalized cation-exchange 0.80 B Flow Rate:
MSA at 24.1 min; equilibration time is 2 min
0.40 mL/min
7
column is tailored for gradient separation of polyvalent and 12
Suppressor: CSRS® ULTRA 2-mm
µS 10
moderately hydrophobic amines, including biogenic amines 2 3 4 6
Cation Self-Regenerating Suppressor,
AutoSuppression® recycle mode, 100 mA
and diamines, using simple aqueous eluents and elevated 0.10 Background: <0.2 µS throughout the chromatogram
0 5 10 15 20 25
temperature (40 °C). The CS17 is an excellent column for use Minutes
Peaks: A B A B
with Reagent-Free IC systems using Eluent Generation, which 1. Lithium
2. Sodium
5
65
– µg/L (ppb)
16
9.
10.
1,6-hexanediamine
N,N-diethylenediamine
250
–
–
200
requires only a deionized water source to produce methanesul- 3. Ammonium
4. Potassium
89
47
4
8
11.
12.
1,7-heptanediamine
N,N,N,N-tetramethyl
125 –
fonic acid eluent. 5. Magnesium

6. Calcium
24
56
–
18
ethylenediamine
13 1,8-octanediamine
–
250
200
–
7. Ethylenediamine – 200 14. 3,3-diaminopropylamine – 200
8. 1,2-propanediamine 500 – 15. 1,9-nonanediamine 375 –
18572
• For polyvalent and moderately hydrophobic amines includ- 16. 1,10-decanediamine 375 –
ing diamines and biogenic amines The IonPac CS17 column demonstrates excellent selectivity for di-
amines.
• Optimized for simple acidic gradient applications with
minimal baseline shift
Comparison of Isocratic and Gradient
• Ideal alternative for IonPac CS14 amine applications 1.6 A 34 Elution on the IonPac® CS17
2
1
• Improved peak shape without adding organic solvent to the µS 5 6 7 8 Column: IonPac CS17, 4-mm
eluents 0.1
Inj. Volume:
Eluent:
25 µL
A. 6 mM Methanesulfonic acid (MSA)
1.6 B Eluent: B. 2 mM MSA isocratic to 11.0 min;
• Compatible with organic solvents step change to 7 mM MSA at 11.1 min;
6 8 isocratic to 22 min; step change back
µS 34 7
to 2 mM MSA at 22.1 min;
12 5
The CS17 column is recommended for polar amines includ- Flow Rate:
equilibration time is 2 min
1.0 mL/min
0.1
ing alkanolamines and methylamines. It can also be used for 0 5 10 15 20 25 Temperature: 30 °C
Minutes Suppressor: Cation Atlas® Electrolytic Suppressor
moderately hydrophobic amines, including biogenic amines, in AutoSuppression® recycle mode,
IC & RFIC Columns
Peaks: 1. Lithium 0.1 mg/L (ppm)

2. Sodium 0.4 24 mA
alkyldiamines, and polyamines. Sample matrices include envi- 3. Ammonium 0.5 Background: < 0.2 µS
ronmental waters, power plant waters treated with ammonium, 4. Potassium 1.0
5. Dimethylamine 1.0
morpholine or ethanolamine, chemical additives, chemical pro- 6. Triethylamine 7.0
7. Magnesium 0.5
cess solutions, scrubber solutions, plating baths, and industrial 8. Calcium 1.0 18569
solvents. The CS17 is also used extensively in the food industry.
Gradient elution improves resolution of cations and amines without
causing a baseline shift.
The CS17 column offers improved peak shapes and efficien-
cies for IonPac CS14 amine applications. Solvent compatibility
permits solvent use for elution of more hydrophobic amines
and easy column cleanup after the analysis of complex sample
matrices. Use the CS17 with Eluent Generation for simplified
methanesulfonic acid eluent preparation.
Note: Use the Cation Self-Regenerating Suppressor (CSRS

300) with the CS17 column.
204 www.dionex.com
IonPac CS17
Determination of Biogenic Amines Using the IonPac® CS17 Ordering Information

Column: IonPac CS17, CG17, 2-mm Temperature: 40 °C
Inj. Volume: 25 µL Suppressor: CSRS® ULTRA 2-mm
Eluent: 3 mM MSA isocratic to 3.5 min; Cation Self-Regenerating Suppressor, Analytical Columns
3 mM to 6 mM MSA gradient at 12 min; AutoSuppression® recycle mode,
isocratic to 15 min; gradient to 40 mM 100 mA
MSA at 20 min, isocratic to 24 min; Background: < 0.2 µS throughout the chromatogram
IonPac CS17 Analytical Column (4 × 250 mm)............................................. 060557
step change back to 3 mM MSA
at 24.1 min; equilibration time is 2 min
Peaks: 1. Lithium 10 µg/L (ppb) IonPac CS17 Analytical Column (2 × 250 mm)............................................. 060561
2. Sodium 40
Flow Rate: 0.40 mL/min 3. Ammonium 50
4. Potassium 100
2.0
7 5. Magnesium 50 Guard Columns
6. Calcium 100
7. Putrescine 1000
8. Cadaverine 600
8
µS 9. Histamine 600
2 3 10. Spermidine 200 IonPac CG17 Guard Column (2 × 50 mm)...................................................... 060563
4 9
6 11. Spermine 400
1 5 10 11
0.1
0 5 10 15 20 25
Minutes 18570
The IonPac CS17 column can easily separate biogenic amines and
Group I and II cations using an aqueous eluent without added organic
solvent.
Determination of Power Plant Amine Additives

Using the IonPac® CS17 Column
Column: IonPac CG17, CS17, 2-mm
Inj. Volume: 25 µL
Eluent: 0.5 mM to 0.7 mM MSA gradient at 25 min;
step change to 4 mM MSA at 25.1 min;
Peaks:
isocratic to 27 min; step change to
1. Lithium 25 µg/L (ppb)
6.5 mM MSA at 27.1 min;isocratic to 37 min;
2. Sodium 100
step change back to 0.5 mM MSA at 37.1 min;
3. Ammonium 125
equilibration time is 2 min
4. Hydrazine 1000
5. Ethanolamine 250
Temperature: 30 °C
6. Potassium 250
Suppressor: Cation Atlas® Electrolytic Suppressor
7. 2-(2-aminoethoxy) ethanol 1000
AutoSuppression® Recycle Mode, 8 mA
8. 5-amino-1-pentanol 500
IC & RFIC Columns

1.30 9. Morpholine 1000
13 10. 2-diethylamino ethanol 1000
7
9 10 12 11. Cyclohexylamine 1000
8
µS 12. Magnesium 125
3 6 11
1 2 45 13. Calcium 250
0
0 10 20 30 40
Minutes 18575
The moderate capacity, hydrophilic IonPac CS17 column solves the

difficult resolution problem of separating hydrazine from ammonium
and ethanolamine.
Related Literature
Product Data Sheets
IonPac CS17 Cation-Exchange Column
Application Updates
AU 155: Determination of Cations and Amines in Hydrogen

Peroxide by Ion Chromatography Using a RFIC (Reagent-Free)
System
AU 160: Determination of N,N-Dimethyl-o-Toluidine and N,N-

Diethyl-o-Toluidine in Ethylene Gas Samples
www.dionex.com 205
IonPac CS16 IonPac® CS16-5 µm (5 × 250 mm)
High-capacity carboxylate-functionalized column for 30 mM Methanesulfonic acid
disparate sodium and ammonium concentrations 1.30
1 2
3
4 Column: IonPac CS16 (5 × 250 mm)
5 6
µS Eluent: See chromatogram
The IonPac CS16 is the column of choice for disparate con- Flow Rate: 1.0 mL/min
Inj. Volume: 25 µL
centration ratios of adjacent eluting cations such as sodium and 0
Temperature: 40 °C
ammonium in diverse sample matrices. The high-capacity, high- 0 5 10 15 20 25 Detection: Suppressed conductivity,
CSRS® ULTRA, 4 mm,
resolution CS16 column can be used for short-chained amines, 48 mM Methanesulfonic acid AutoSuppression® recycle mode
including alkylamines and alkanolamines, in diverse sample 1.30

1 2 3 5 4 6
Peaks: 1.
2.
Lithium
Sodium
0.1 mg/L (ppm)
0.4
matrices. µS
3. Ammonium 0.5
4. Potassium 1.0
5. Magnesium 0.5
0
• For disparate concentration ratios of close-eluting cations 6. Calcium 1.0
such as ammonium and sodium in complex matrices 0 5

Minutes
10
16170
• Ideal for trace-level ammonium in high concentrations of

sodium Isocratic separation of common inorganic cations and ammonium.
Note that magnesium elutes before potassium at the higher eluent
• Ideal for trace-level sodium in high concentrations of am- concentration.
monium or amines
Trace Ammonium in a High Sodium Sample
• Best carboxylate-functionalized column to tolerate samples Column A: IonPac CS16 (5 × 250 mm)
0.08 A
with low pH 1
Column B:
Eluent A:
IonPac CS16 (3 × 250 mm)
30 mM Methanesulfonic acid
2 Eluent B: 7 mM Methanesulfonic acid
µS
• Isocratic acid eluent and elevated temperature (40 °C) for 23 min to 52 mM at 28 min
Flow Rate A: 1.0 mL/min
required for sodium:ammonium ratios to 1:10,000 0 Flow Rate B: 0.43 mL/min
Temperature: 40 °C
0 2 4 6 8 10 Inj. Volume: 25 µL
• Gradient MSA eluent and elevated temperature required for Detection: Suppressed conductivity,
sodium:ammonium ratios up to 1:20,000 0.40 B CSRS®-ULTRA,
Peaks A: 1. Sodium 100 mg/L (ppm)
• Useful for short-chained amines, including alkylamines and
IC & RFIC Columns
µS 2 2. Ammonium 0.01
alkanolamines Peaks B: 1. Sodium 500
0 2. Ammonium 0.025
• Compatible with organic solvents excluding alcohols CTC-1 Column placed between the Cell Out
0 5 10 15 20
Minutes and Regen In Ports
Using an isocratic acid eluent and elevated temperature (40 °C) 17493

coupled with suppressed conductivity, ratios up to 10,000:1 of
sodium and ammonium can be resolved in less than 20 min. Isocratic versus gradient determination of trace level ammonium in a
high sodium sample.
Sample matrices include environmental waters; power plant
waters treated with ammonium, morpholine or ethanolamine;
chemical additives; chemical process solutions; scrubber
solutions; plating baths; and industrial solvents. The CS16 is
designed for use in most IonPac CS15 applications.
The CS16 column has the highest capacity among Dionex

carboxylate-based cation columns, resulting in improved load-
ability and resolution. Use the CS16 with the Eluent Generator
for simplified, consistent methanesulfonic acid eluent prepara-
tion. Use the Cation Self-Regenerating Suppressor (CSRS 300)
with the CS16 column.
206 www.dionex.com
IonPac CS16
Application Notes
Determination of Trace-Level Sodium in a High Ammonium
Sample Using the IonPac® CS16 Column AN 94: Determination of Trace Cations in Concentrated Acids
0.07 2
Column: IonPac CS16 (5 × 250 mm) AN 141: Determination of Inorganic Cations and Ammonium
Eluent:
Temperature:
30 mM Methanesulfonic acid
40 °C
in Environmental Waters by Ion Chromatography Using the
µS
1 Flow Rate: 1.0 mL/min IonPac CS16 Column
Sample: Industrial wastewater, 1:10 dilution
Inj. Volume: 25 µL
0
Detection: Suppressed conductivity, AN 152: Determination of Sodium at the Parts-Per-Trillion
CSRS® ULTRA, 4 mm,
AutoSuppression® recycle mode Level in the Presence of High Concentrations of Ethanolamine
Peaks: 1. Sodium 0.01 mg/L (ppm) in Power Plant Waters
0 5 10 15 20 25 2. Ammonium 100
Minutes
AN 157: Comparison of suppressed to nonsuppressed con-
ductivity detection for the determination of common inorganic
16391
cations
Isocratic determination of a 1:10,000 sodium to ammonium concentra-
tion ratio.
Trace Sodium in a High Amine Sample
Column: IonPac® CS16 (3 × 250 mm) Analytical Columns
Eluent: 7 mM Methanesulfonic acid
for 23 min to 52 mM at 28 min IonPac CS16 Analytical Column (5 × 250 mm)............................................. 057573
2.7 1 Temperature: 40 °C
4 Flow Rate: 0.43 mL/min IonPac CS16 Analytical Column (3 × 250 mm)............................................. 059596
Inj. Volume: 25 µL
CSRS®-ULTRA 2-mm,
µS AutoSuppression® recycle mode Guard Columns
Peaks: 1. Lithium 2500 µg/L (ppb)
5 6 7 8
2. Sodium 6 IonPac CG16 Guard Column (5 × 50 mm)...................................................... 057574
3 3. Ammonium 100
2
0 4. Ethanolamine 2000 IonPac CG16 Guard Column (3 × 50 mm)...................................................... 059595
5. Hydrazine 4000
IC & RFIC Columns

0 5 10 15 20 25 30 35 6. Potassium 4
Minutes 7. Magnesium 1
8. Calcium 6
CTC-1 Column placed between the Cell Out

and Regen In Ports
17494
Determination of trace level sodium in a high amine sample.
Related Literature
Product Data Sheets
IonPac CS16 Cation-Exchange Column Data Sheet
www.dionex.com 207
IonPac CS15 Isocratic Separation of Ethanolamine
Medium-capacity cation-exchange column for Using the IonPac® CS15 Column
disparate concentration ratios of sodium and Column: IonPac CG15, CS15 4-mm
ammonium 5
Eluent: 10 mN Sulfuric acid /
9% Acetonitrile
Temperature: 40 °C
The IonPac CS15 is a medium-capacity carboxylate-function- Inj. Volume: 25 µL
µS 1
alized cation-exchange column with crown ether moiety for the 4
5
ASRS® ULTRA 4-mm,
determination of disparate concentration ratios of sodium and 7 AutoSuppression® external water mode
3
ammonium in diverse sample matrices. 0
Peaks: 1.
2.
Lithium
Sodium
1.0 mg/L (ppm)
4.0
3. Ethanolamine 3.0
4. Ammonium 5.0
• Solvent and elevated temperature required for more ef- 0 5 10 15 20 25
5. Magnesium 5.0
Minutes
6. Calcium 10.0
ficient ammonium and potassium peaks 7. Potassium 10.0
• Use the CS16 column for separating disparate concentra- 12829
tions of sodium and ammonium without an organic solvent

The unique selectivity of the CS15 column causes ethanolamine to elute
• Useful for determination of trace ethanolamine in high- before ammonium, and potassium to elute after the divalent magnesium
ammonium or high-potassium concentrations and calcium cations.
• Useful for separating alkanolamines and other small amines

Isocratic Determination of Ammonium in the Presence of
• The crown ether moiety gives it unique selectivity, and may High Sodium (1:4000 ratio) with the IonPac® CS15 Column
offer an advantage for certain types of matrices
• Compatible with organic solvent Column: IonPac CG15, CS15, 4 mm
.08 1 3 Eluent: 10 mN Sulfuric acid/
9% Acetonitrile
The IonPac CS15 has unique selectivity, and is the only car- 2 Temperature: 40 °C
boxylic acid based cation column from Dionex in which etha- µS Inj. Volume: 25 µL
nolamines elute before ammonium. Thus, for samples with high CSRS® ULTRA, 4 mm,
IC & RFIC Columns

ammonium to low ethanolamine ratios, the CS15 is the column 0 Peaks: 1. Sodium 100 µg/L (ppm)
of choice. Using an isocratic acid or solvent eluent and elevated 0 2 4 6 8 10 12 14 16 18 20
Minutes
2. Ammonium 0.025
3. Calcium -
temperature (40 °C), coupled with suppressed conductivity
detection, ratios of up to 8000:1 of sodium and ammonium can
also be determined. 12826
Sample matrices include environmental waters; power plant Isocratic determination of trace-level ammonium in environmental
wastewater containing a high sodium concentration.
waters treated with ammonium, morpholine or ethanolamine;
chemical additives; chemical process solutions; scrubber solu-
tions; plating baths; industrial solvents; and soil matrices. The
CS15 is also particularly useful for matrices with high potas-
sium concentrations. Use the Cation Self-Regenerating Suppres-
sor (CSRS 300) with the CS15 column.
Note: See the IonPac CS16 for improved performance without

using solvent.
208 www.dionex.com
IonPac CS15
Trace Ammonium in a Potassium Chloride Soil Extract
2 Column: IonPac CG15, CS15, 4 mm

Analytical Columns
0.14
Eluent: 10 mN Sulfuric acid/9% Acetonitrile
Flow Rate: 1.2 mL/min IonPac CS15 Analytical Column (4 × 250 mm)............................................. 051795
Temperature: 40 ºC
µS
Inj. Volume: 25 µL
1 Detection: Suppressed conductivity, CSRS-ULTRA
4-mm, AutoSuppression external water
0.0 mode Guard Columns
0 5 10 15 20 25 Peaks:
1. Ammonium 0.01 mg/L (ppm)
Minutes IonPac CG15 Guard Column (4 × 50 mm)...................................................... 052200
2. Potassium 100
12958
Determination of trace-level ammonium in a potassium chloride soil

extract.
Step—Change Elution of 20,000:1 Ratio of Sodium to Ammonium
0.80 1
Column: IonPac® CS15 (2 × 250 mm)
Eluent: 3 mM MSA step to 15 mM MSA
at 13 min; step to 3 mM MSA
at 23 min, for 10 min.
9% acetonitrile (constant)
µS 2 Flow Rate: 0.3 mL/min
Inj. Volume: 25 µL
Temperature: 40 °C
CSRS® ULTRA, 2 mm,
IC & RFIC Columns

0.30 Peaks: 1. Sodium 500,000 mg/L (ppm)
0 5 10 15 2. Ammonium 25
Minutes
17097
Step change elution of 20,000:1 ratio of sodium to ammonium.
Related Literature
Product Data Sheets
IonPac CS15 Cation-Exchange Column Data Sheet
www.dionex.com 209
IonPac CS14 Gradient Elution of Alkylamines and the
Group I and II Cations on IonPac CS14
Medium low-capacity column for aliphatic and Peaks: 1. Lithium 0.5 mg/L (ppm)
IonPac® CS14 4 mm
aromatic amines and polyamines Column:
Eluent: Methanesulfonic acid/
2.
3.
Sodium
Ammonium
2
2.5
Acetonitrile gradient 4. Methylamine 5
Flow Rate: 1 mL/min 5. Ethylamine 5
The IonPac CS14 is a medium low-capacity carboxylate- Inj. Volume: 25 µL 6. Dimethylamine 10
functionalized cation-exchange column for the determination CSRS® ULTRA, 4 mm,
7. Potassium 0.5
8. Trimethylamine 20
of aliphatic, aromatic, and polyvalent amines. Sample matrices 9. Propylamine 5
10. Morpholine 25
include environmental waters; power plant waters treated with 5 56 7
14
11. sec- Butylamine 10
4 12. iso-Butylamine 10
ammonium, morpholine or ethanolamine; chemical additives; 2 8 13
15 17 19 13. Magnesium 2.5
1 18
16
chemical process solutions; scrubber solutions; plating baths; µS
3
9 11
12 20
14.
15.
Calcium
2-Methylpiperidine 20
5
10
industrial solvents; and wastewater. 16. Di-n-propylamine 20
17. 3-Methylpiperidine 20
18. Cyclohexylamine 20
0
• For amine separations, including aliphatic amines, aromatic 0 10 20 30
19.
20.
N-Methylcyclohexylamine 20
Tripropylamine 20
Minutes 9102-01
amines, and polyamines
• For hydrophobic and polyvalent amines The unique selectivity of the IonPac CS14 allows a large number of
aliphatic amines and Group I and Group II cations to be resolved.
• For polar amines including alkanolamines and alkylamines
• For moderately hydrophobic amines
Related Literature
• Compatible with organic solvents
The CS14 column can be used with isocratic or gradient meth- PDF documents under Literature on www.dionex.com.
anesulfonic acid or sulfuric acid eluents to resolve a variety of
amines from the Group I and II cations. Solvent and elevated Product Data Sheets
temperature may be required for efficient elution of hydro-
phobic amines. Use the Cation Self-Regenerating Suppressor IonPac CS14 Cation Exchange Column Data Sheet
(CSRS 300) with the CS14 column.
IC & RFIC Columns
Application Notes
The CS18 and CS17 are recommended replacement columns AN 86: Determination of Trace Cations in Power Plant Waters
for most CS14 applications. The CS17 is recommended replace- Containing Morpholine
ment column for hydrophobic and polyvalent amines, includ-
ing biogenic amines and diamines. The CS18 is recommended AN 148: Determination of Bethanechol by Ion Chromatography
for polar amines, including alkanolamines and methylamines;
and moderately hydrophobic and polyvalent amines, including
biogenic amines and alkyldiamines. Ordering Information
Analytical Columns
Step Change Separation of Ethyl, Diethyl, and Triethylamine
Plus the Group I & II Cations Using the IonPac® CS14 Column IonPac CS14 Analytical Column (2 × 250 mm)............................................. 044121
Column: IonPac CS14
4 Eluent: 9 mM Methanesulfonic acid
2 + 0.5% Acetonitrile; step change at
8 6 min to 11 mM Methanesulfonic acid Guard Columns
1 45 + 1.5% Acetonitrile
3 9 Flow Rate: 1 mL/min IonPac CG14 Guard Column (2 × 50 mm)...................................................... 044122
µS
Detection: Suppressed conductivity IonPac CG14 Guard Column (4 × 50 mm)...................................................... 044124
6 Peaks: 1. Lithium 0.5 mg/L (ppm)
2. Sodium 2
3. Ammonium 2.5
0 4. Ethylamine 10
5. Potassium 5
0 5 10 15 20 6. Diethylamine 10
Minutes 7. Magnesium 2.5
8. Calcium 5
9. Triethylamine 50
8921
Acetonitrile can be used to optimize the resolution of ethylamines from

Group I and Group II cations.
210 www.dionex.com
IonPac CS12A
IonPac CS12A Isocratic Separation of Morpholine, Alkali, and
Medium capacity column for fast, isocratic Alkaline Earth Metals on IonPac CS12A
separation of cations using MSA or sulfuric acid 8
Column: IonPac CS12A

The IonPac CS12A is a medium-capacity carboxylate-func- Eluent: 20 mN Sulfuric acid
tionalized cation-exchange column recommended for the fast, 2 Inj. Volume: 25 µL
isocratic separation of lithium, sodium, ammonium, potassium, µS 1 3 7
CSRS AutoSuppression
6
magnesium, and calcium using methanesulfonic or sulfuric acid Peaks: 1. Lithium 0.5 mg/L (ppm)
2. Sodium 2.0
eluents in diverse sample matrices. 5 3. Ammonium 2.5
4. Potassium 5.0
5. Morpholine 25.0
0
• Robust, reliable column for group I and II cations plus am- 6. Magnesium 2.5
7. Calcium 5.0
monium 0 5 10 15
Minutes
• Recommended for manganese and morpholine 11196-01
• Recommended for inorganic cations in complex matrices Power plant waters treated with morpholine and ammonium are well
resolved on the IonPac CS12A column with an isocratic eluent.
• Simplified Reagent-Free IC operation provided by the EG
or eluent regeneration
Fast Cation Separations with CS12A-5µm (3 x 150 mm)
• Compatible with organic solvents (except alcohols), high
temperatures, and high acid concentrations 6.5 4 Eluent A: 33 mM Methanesulfonic acid
A 2 7 8
1 3 Flow Rate A: 0.8 mL/min
• Analysis time for common six cations as short as 3 min Eluent B: 20 mM Methanesulfonic acid
Flow Rate B: 1.0 mL/min
(CS12A-5µm) to 15 min (CS12A-8µm) Inj. Volume: 25 µL
Temperature: 30 °C
• The CS12A-MS column offers fast elution at the low flow 0 1 2 3 AMMS®-ICE Suppressor
rates required for IC-MS, making it ideal for IC-MS 4.6 B
5 Peaks: 1. Lithium 6. Cesium
screening 2 2. Sodium 7. Magnesium
6 9 3. Ammonium 8. Calcium
4
IC & RFIC Columns

µS 3 4. Potassium 9. Strontium
• For disparate concentration ratios of adjacent-eluting cat- 1 7
8
10 5. Rubdium 10. Barium
ions, the high-capacity IonPac CS16 is recommended
0
0 2 4 6 8 10
The medium-capacity IonPac CS12A cation-exchange column 15640
supports fast, isocratic separation with suppressed conductivity
detection. Sample matrices include environmental and drinking Fast cation separations using the IonPac CS12A-5µm.
waters; soil extracts; acid digests; power plant waters treated
with ammonium or morpholine; chemical additives; chemical
process solutions; scrubber solutions; plating baths; and indus-
trial solvents.
Use the CS12A-5µm (3 × 150 mm) column for high efficiency

and fast analysis (3 min). The smaller-diameter resin technology
and reduced column length provide faster analysis time, reduced
eluent consumption, and increased sensitivity. The CS12A
column is the recommended replacement for all CS12 applica-
tions. Use the CS12A with the Eluent Generator for simplified
methanesulfonic acid eluent preparation.
Note: Use the Cation Self-Regenerating Suppressor

(CSRS 300) with the CS12A column.
www.dionex.com 211
Application Updates
Eluent Step Change for 1000:1 Sodium-to-Ammonium
Concentration Ratios Using the IonPac® CS12A Column AU 137: Determination of Trace Lithium in Industrial Process
Waters
Column: IonPac CS12A, 4 mm
30
2
Eluent: Step change at 12 min
from 3 mN to 20 mN
AU 158: Determination of Manganese in Brine
Methanesulfonic acid
CSRS® ULTRA, 4 mm,
4 5 6 Peaks: 1. Lithium 0.50 mg/L (ppm)
1
2. Sodium 52.0
0
0 5 10 15
3. Ammonium
4. Potassium
0.05
5.0
Analytical Columns
Minutes 5. Magnesium 2.5
6. Calcium 5.0 IonPac CS12A-5µm Analytical Column (3 × 150 mm).................................. 057185
11340
IonPac CS12A-MS Analytical Column (2 × 100 mm) .................................. 059960
IonPac CS12A Analytical Column (4 × 250 mm)........................................... 046073
Trace-level quantification of ammonium in brine (1000:1 ratio) using IonPac CS12A Analytical Column (2 × 250 mm)........................................... 046075
the IonPac CS12A coupled with cation AutoSuppression.
Guard Columns
Small Amines by IC-MS
IonPac CG12A-5µm Guard Column (3 × 30 mm)........................................... 057184
6 IonPac CG12A Guard Column (2 × 50 mm)................................................... 046076
Column: IonPac® CS12A-MS, 2 × 100 mm
5 ®
Suppressor: CSRS ULTRA, external water, 10 mA IonPac CG12A Guard Column (4 × 50 mm)................................................... 046074
4 Gradient: 2–18% CH3CN, 10–25 mM
MSA in 6 min, hold 2 min
3 Flow rate: 0.25 mL/min
2 Injection Loop: 5 µL
Detection: AQA™ MS, +ESI, 20 V,
1 200 °C, SIM as indicated
+Electrospray MS Peaks: m/z mg/L

1. Ethanolamine 62 50
2. Diethanolamine 106 50
4 Conductivity 3,4,5
IC & RFIC Columns
3. Triethanolamine 150 25,000

µS 4. Trimethylamine 60 5,000
Na+ 6 5. Ethyldimethylamine 74 500
1,2
0 6. Triethylamine 102 50
0 2 4 6
Minutes
17108-01
Determination of alkylamines and alkanolamines using IC-MS detec-

tion.
Related Literature
Product Data Sheets
IonPac CS12A Cation-Exchange Column Data Sheet
Application Notes
AN 107: Ions In Physiological Fluids
AN 120: Determination of Calcium and Magnesium in Brine
AN 124: Determination of Choline in Dry Milk and Infant

Formula
212 www.dionex.com
IonPac CS11
IonPac CS11 Isocratic Separation of Alkali Metal, Alkaline Earth,
Sulfonate-functionalized cation-exchange column for and Ammonium Cations Using the IonPac® CS11 Column
isocratic separations using HCl and DAP.
10 5
Column: IonPac CS11 2-mm
The IonPac CS11 is designed for the isocratic separation of in- Eluent: 50 mM Hydrochloric Acid /
2 7 mM Diaminopropionic Acid
organic cations using hydrochloric acid with diaminopropionic Hydrochloride
(DAP) acid eluents. µS
1
3 Peaks: 1. Lithium
6
• The CS11 can be used for analysis of common inorganic 2. Sodium
3. Ammonium
cations and aliphatic, cyclic, and aromatic amines. 4. Potassium
5. Magnesium
–2 6. Calcium
• Compatible with organic solvents 0 5 10 15
Minutes
• The CS12A improves performance for fast analysis of

7411
inorganic cations and ammonium.
Common inorganic cations can be separated using the IonPac CS11
• The CS17 column is recommended for hydrophobic and
with a HCl/DAP acid eluent.
polyvalent amines.
• The CS18 column is recommended for polar amines
including alkanolamines and methylamines. Related Literature
• The CS18 is also recommended for moderately For detailed specifications and applications, see the following
hydrophobic amines and polyvalent amines. PDF documents under Literature on www.dionex.com.
The CS11 is useful in determining common inorganic cations

and aliphatic and aromatic amines in diverse sample matrices, Application Updates
including environmental and drinking waters, power plant wa- AU 138: Determination of Ethanolamines in Industrial Waters
ters treated with ammonium, soil extracts, acid digests, chemical by Cation-Exchange Chromatography
IC & RFIC Columns

additives, chemical process solutions, scrubber solutions, plat-
ing baths, and industrial solvents.
The CS11 has selectvitiy similar to the CS10 column, with
improved sensitivity and lower eluent consumption due to its Analytical Columns
smaller 2 mm i.d. format.
Note: Use the Cation MicroMembrane Suppressor
(CMMS 300) with the CS11 column. Electrolytic suppression is Guard Columns
not recommended with HCl and DAP eluents. IonPac CG11 Guard Column (2 × 50 mm)...................................................... 043128
www.dionex.com 213
IonPac CS10
Determination of Biogenic Amines in Emmenthal Cheese
Sulfonate-functionalized cation-exchange column for Using the IonPac® CS10 Column
isocratic separations using HCl and DAP.
100
Column: IonPac CG10, CS10 4-mm
The IonPac CS10 is a sulfonate-functionalized cation-exchange Eluent: 1 M Sodium perchlorate, 0.375 M
column for the isocratic separation of inorganic cations using 2 Flow Rate:
perchloric acid, water (81:5:14, v/v/v)
1 mL/min
hydrochloric acid with diaminopropionic (DAP) acid eluents. nC
1 Detection: Amperometry, gold working electrode
Peaks: 1. Putrescine 4.9 µg/g (ppm)
The CS12A is the recommended column for the fast analysis 3
5 2. Histidine 13.5
of inorganic cations; the CS17, for hydrophobic and polyvalent 4 3. Tyrosine
4. Cadaverine
12.6
2.6
amines; and the CS18, for polar amines. The CS18 is also rec- –10
5. Tyramine 12.7
Reference: R. Draisci et. al. J. Chromatogr. A
ommended for moderately hydrophobic and polyvalent amines. 0 10 20 30
798 (1998) 109–116
Minutes
• The CS10 can be used for analysis of the common inorgan-

14305
ic cations and aliphatic, cyclic, and aromatic amines.
• Compatible with organic solvents. Biogenic amines can be separated rapidly using the IonPac CS10
column.
• The CS12A provides improved performance for fast analy-
sis of inorganic cations and ammonium.
Related Literature
• The CS17 column is recommended for hydrophobic and
polyvalent amines. For detailed specifications and applications, see the following
• The CS18 column is recommended for polar amines in- PDF documents under Literature on www.dionex.com.
cluding alkanolamines and methylamines.
Product Data Sheets
• The CS18 is also recommended for moderately hydropho-
bic amines and polyvalent amines. IonPac CS10 Cation Exchange Column Data Sheet
IC & RFIC Columns
The IonPac CS10 is designed for the isocratic separation of

lithium, sodium, ammonium, potassium, magnesium, and Ordering Information
calcium using hydrochloric acid with diaminopropionic acid
eluents with suppressed conductivity detection. Sample matrices
Analytical Columns
for the CS10 include environmental waters; power plant waters
treated wtih ammonium, morpholine, or ethanolamine; chemical IonPac CS10 Analytical Column (4 × 250 mm)............................................. 043015
additives; chemical process solutions; scrubber solutions; plat-
ing baths; and industrial solvents. Guard Columns
The CS11 has selectvitity similar to the CS10 column, with
improved sensitivity due to its smaller 2 mm i.d. format.
Note: Use the Cation MicroMembrane Suppressor (CMMS

300) with the CS10 column. Electrolytic suppression is not
recommended with HCl and DAP eluents.
214 www.dionex.com
Transition Metal Columns
For determination of metals in diverse sample
matrices.
Cation-exchange chromatography is ideal for determination of
transition and lanthanide metals in a variety of sample matrices.
Because most transition and lanthanide metals are incompatible
with suppressed conductivity detection, postcolumn reagent
absorbance detection is typically used. Dionex transition metal
columns are optimized for this mode of operation.
IonPac CS5A: High-resolution, ion-exchange column for deter-
mination of transition and lanthanide metals in diverse sample
matrices.
IC & RFIC Columns
www.dionex.com 215
IonPac CS5A Anion Exchange Separation of Lanthanides
Using the IonPac® CS5A Column
High-resolution, ion-exchange column for transition Column: IonPac CG5A, CS5A 4-mm
and lanthanide metals in diverse sample matrices Eluent:
Flow Rate:
Oxalic acid, diglycolic acid gradient
1.2 mL/min
Inj. Volume: 50 µL
0.3 Detection: Absorbance, 520 nm with PAR
The IonPac CS5A is a high-resolution, ion-exchange column in MetPac™ Postcolumn Reagent Diluent
designed for determination of transition and lanthanide metals 7
89
1011 Peaks: 1. Lanthanum 5 mg/L (ppm)
6 2. Cerium 5
in a variety of sample matrices. The CS5A column, in combi- AU 5
12
3. Praseodymium 5
13
4. Neodymium 5
nation with postcolumn derivitization and visible detection at 3
4 14
5. Samarium 5
2
530 nm, provides a sensitive and selective method for transition 0.0
1 6. Europium 5
7. Gadolinium 5
metal analysis. 8. Terbium 5
0 2 4 6 8 10 12 9. Dysprosium 5
Minutes 10. Holmium 5
• Recommended for the separation of transition and lan- 11. Erbium 5
12. Thulium 5
thanide metals 13. Ytterbium 5
14. Lutetium 5 12159
• Also useful for aluminum analysis
Separation of lanthanide metals using oxalic acid/diglycolic acid as the
The CS5A column provides simultaneous determination of complexing agent.
common transition metals in less than 11 min using a unique
bifunctional resin. The transition metals are detected following Determination of Transition Metals in Sea Water Using
complexation with 4-(2-pyridylazo) resorcinol (PAR). The PAR Chelation Ion Chromatography and the IonPac® CS5A Column
0.02 Spiked Sea Water
derivative is detected at 520–530 nm. This method is both sensi-
4
Columns: IonPac CG5A, CS5A, 4 mm,
tive and selective for transition metals. 1 5 7 MetPac™ TMC-1
2 3 6
AU Eluent: PDCA, isocratic
The CS5A provides improved selectivity and higher efficiencies Preconc. Vol.: 2.5 mL
Detection: Visible, 530 nm
than its predecessor, the CS5 column. Use the MetPac PDCA –0.01 Postcolumn
Reagent: PAR at 0.6 mL/min
and Oxalic Acid Eluent Concentrates and PAR Postcolumn 0.02
Sea Water Peaks: Sea Water Spike Amt. Spiked Sea Water
Reagent Diluent for simplified operation. 1. Iron 10.8 mg/L 10.0 mg/L 21.6 mg/L (ppb)
7 2. Copper - 2.50 2.42
IC & RFIC Columns
1 4
AU 3. Nickel 1.60 5.00 5.85
3 4. Zinc 1.88 5.00 6.78
5. Cobalt - 2.50 2.57
6. Cadmium - 12.5 12.5
7. Manganese 7.16 7.50 14.3
–0.01
0 2 4 6 8 10 12 14
Minutes 12427
Determination of transition metals in seawater.
216 www.dionex.com
IonPac CS5A
Separation of Transition Metals Using Oxalic Acid
with the IonPac® CS5A
Analytical Columns
3
Column: IonPac CS5A, CG5A 4-mm IonPac CS5A Analytical Column (4 × 250 mm)............................................. 046100
0.2 Eluent: MetPac™ Oxalic Acid eluent
Flow Rate: 1.2 mL/min IonPac CS5A Analytical Column (2 × 250 mm)............................................. 052576
Inj. Volume: 50 µL
1 2 Detection: Absorbance, 530 nm with PAR in
5 6
AU 4 MetPac Postcolumn Reagent Diluent
Peaks 1. Lead 5.0 mg/L (ppm)
Guard Columns
2. Copper 0.70
0.0 3. Cadmium 3.3 IonPac CG5A Guard Column (4 × 50 mm)..................................................... 046104
4. Cobalt 0.70
0 2 4 6 8 10 12
5. Zinc 1.3 IonPac CG5A Guard Column (2 × 50 mm)..................................................... 052836
6. Nickel 2.0
Minutes
11871
Separation of transition metals by a mixed-mode mechanism using

oxalate as a complexing agent.
Large Sample Loop Injection of µg/L Levels of Transition Metals

Using the IonPac® CS5A Column
Columns: IonPac CG5A, CS5A, 2-mm

0.020 4 Eluent: MetPac™ PDCA
Sample 1 Flow Rate: 0.3 mL/min
2 Post Column
AU 5 7
8 Reagent: PAR in MetPac Postcolumn
3 6
Reagent Diluent
PCR Flow Rate: 0.15 mL/min
–0.005
Detection: 530 nm, 10 mm cell
0.020 Temp: 30 °C
Blank Inj Volume: 1000 µL
Peaks: 1. Iron 2 µg/L (ppb)
AU
IC & RFIC Columns

4 2. Copper 1
3. Nickel 2
–0.005 4. Zinc >2
0 2 4 6 8 10 12 14 16 5. Cobalt 1
Minutes 6. Cadmium 5
7. Manganese 3
8. Iron 2
13775
Large sample loop injection of µg/L levels of transition metals on a

2 mm system with PC10 pneumatic postcolumn delivery.
Related Literature
Product Data Sheets
IonPac CS5A Column and MetPac Reagents Data Sheet
Application Notes
AN 131: Determination of Transition Metals at PPT Levels in

High-Purity Water and SC2 (D-clean) Baths
AN 108: Determination of Transition Metals in Serum and

Whole Blood by Ion Chromatography
Technical Notes
TN 10: Determination of Transition Metals by Ion

Chromatography
www.dionex.com 217
IC & RFIC Columns
218 www.dionex.com
Ion-Exclusion Columns
Ion-exclustion for organic acid separations
In ion-exclusion separations, Donnan exclusion causes strong
acids to elute in the void volume of the column. Weak acids that
are protonated in the acidic eluent are not subject to Donnan
exclusion and penetrate into the pores of the resin. Separation is
accomplished by differences in pKa, size, and hydrophobicity.
IonPac ICE-AS1: Ion-exclusion column for fast analysis of

aliphatic organic acids and alcohols in complex samples.
IonPac ICE-AS6: Ion-exclusion column for aliphatic organic
acids, including hydroxy-substituted organic acids, and alcohols
in complex samples
IonPac ICE-Borate: Ion-exclusion column ideal for monitoring
trace levels of borate in high-purity water.
IC & RFIC Columns
www.dionex.com 219
IonPac ICE-AS1 Determination of Organic Acids in a
Ion-exclusion column for fast analysis of aliphatic Nitric Acid Sample Using the IonPac® ICE-AS1
organic acids and alcohols in complex samples

100
Nitrate
The IonPac ICE-AS1 ion-exclusion column supports fast Column: IonPac ICE-AS1 (9 × 250 mm)
Eluent: 1 mM Octanesulfonic acid
analysis of aliphatic organic acids and alcohols in complex or Flow Rate: 0.8 mL/min
Detection: Suppressed conductivity, AMMS® ICE
high-ionic-strength samples, including foods and beverages, µS Sample: 52% Nitric acid, diluted to 1%
biological samples, fermentation processes, industrial process 2 Peaks: 1. Succinic acid
1 2. Glutaric acid
liquors, and wastewaters. 3 3. Adipic acid
Ion exclusion allows the determination of organic acids
0 in nitric acid and eliminates nitrate interference.
• Fast separation of organic acids The nitrate ion elutes in the void volume.
0 15 30
Minutes
• Ideal for electroactive ions such as cyanide and sulfite
12594
• Useful for organic acids and alcohols in complex sample
matrices Determination of organic acids in a nitric acid sample using the Ion-
Pac ICE-AS1.
The IonPac AS11 is recommended for fast analysis of organic
acids and inorganic anions in well-characterized samples. Use
the high-capacity AS11-HC for organic acids and inorganic Selectivity for C1–C6 Organic Acids on ICE-AS1
anions in complex sample matrices. 5
Column: IonPac® ICE-AS1 (9 × 250 mm)

With the IonPac ICE-AS1 column, weakly ionized acids are 3
Eluent: 0.4 mM Heptafluorobutyric acid
separated by pKa differences, size, and hydrophobicity. Strong 4 Inj. Volume: 50 µL
acid anions such as chloride, oxalate, and sulfate elute in the 1 5 Detection: Suppressed conductivity, AMMS® ICE
µS Peaks: 1. Formic acid
void and do not interfere with quantification of the organic 2. Acetic acid
6
acids. The ICE-AS1 column can be used with any typical strong 3. Propionic acid
4. Butyric acid
acid eluent and a wide variety of detectors, including conductiv- 5. Valeric acid
IC & RFIC Columns
6. Caproic acid
ity, amperometry, photometry, and refractive index, to determine
aliphatic organic acids.
0
0 10 20 30 40
Minutes
Note: Use the Anion MicroMembrane Ion-Exclusion 9982
Suppressor (AMMS ICE 300) with the ICE-AS1 column.

Selectivity of ICE-AS1 for C1-C6 organic acids.
220 www.dionex.com
IonPac ICE-AS1
Application Notes
Determination of Weakly Ionized Organic Acids
Using UV Detection AN 21: Organic Acids in Wine
8 7 AN 54: Determination of Sulfite in Food and Beverages by Ion

Column: IonPac® ICE-AS1 (9 × 250 mm) Exclusion Chromatography with Pulsed Amperometric Detec-
Eluent: 5 mM Sulfuric acid
Flow Rate 0.8 mL/min tion
1 4 Detection: UV, 210 nm
Peaks: 1. Tartaric acid
mAU
2. Quinic acid
AN 116: Quantification of Anions in Pharmaceuticals
3
2 5 3. Succinic acid
6 8 4. Formic acid
9
5. Acetic acid
AN 117: Quantification of Carbohydrates and Glycols in Phar-
6. Adipic acid maceuticals
0 7. Acrylic acid
8. Butyric acid
0 10 20 30 9. Valeric acid AN 188: Determination of Glycols and Alcohols in Fermenta-
Minutes
tion Broths Using Ion-Exclusion Chromatography and Pulsed
12596 Amperometric Detection
Determination of organic acids using the IonPac ICE-AS1 column.
Determination of Alcohols Ordering Information
60
6
Column:
Eluent:
IonPac ICE-AS1 (9 × 250 mm)
50 mM Perchloric acid
IonPac ICE-AS1
Flow Rate 0.8 mL/min
9
Inj. Volume: 25 µL IonPac ICE-AS1 Analytical Column (9 × 250 mm)........................................ 043197
Detection: Integrated amperometry, Pt electrode
3 Peaks: 1. Sorbitol 50 mg/L IonPac ICE-AS1 Analytical Column (4 × 250 mm)........................................ 064198
2 5 7 2. Xylitol 50
nC 1 4 3. Erythritol 50 IonPac ICE-AS1 Guard Column (4 × 50 mm)................................................. 067842
11 12 4. Glycerol 20
8 5. Ethylene glycol 20
10 6. Methanol 50
0 7. Ethanol 50
8. 2-Propanol 200
IC & RFIC Columns

9. 1-Propanol 200
0 5 10 15 20 25 30 35 40 10. 2-Butanol 200
Minutes 11. 1-Butanol 100
12. 3-Methyl-1-propanol 300
12597
Determination of alcohols using the IonPac ICE-AS1 with pulsed

amperometric detection.
Related Literature
Product Data Sheets
IonPac ICE-AS1 Ion-Exclusion Column Data Sheet
www.dionex.com 221
IonPac ICE-AS6 Separation of Low-Molecular-Weight Organic Acids
Ion-exclusion column for aliphatic organic acids and Using the IonPac® ICE-AS6 Column
alcohols in complex samples Column: IonPac ICE-AS6
10 Eluent: 0.4 mM heptafluorobutyric acid
The IonPac ICE-AS6 ion-exclusion column is designed for the Inj. Volume: 50 µL
10
fast analysis of aliphatic organic acids (including hydroxy- Detection: Suppressed conductivity,
AMMS® ICE
4 9
substituted organic acids) and alcohols in complex or high-ion- 3 6 Peaks: 1. Oxalic 5.0 mg/L (ppm)
µS 2. Tartaric 10.0
ic-strength samples, including foods and beverages, biological 1
2 8 3. Citric 15.0
samples, fermentation processes, industrial process liquors, and 5 7
4. Malic 20.0
5. Glycolic 10.0
wastewaters. The ICE-AS6 column is ideally suited for most 6. Formic 10.0
0 7. Lactic 10.0
applications performed on the ICE-AS1 column. 0 5 10 15 20 8. HIBA 30.0
Minutes 9. Acetic 25.0
10. Succinic 25.0
• Use the ICE-AS6 column for organic acids in complex
14427
sample matrices.
• Use the ICE-AS1 column for the fast separation of organic Separation of low-molecular-weight organic acids using the IonPac
ICE-AS6 column.
acids.
The ICE-AS6 column provides improved peak efficiencies and Determination of Trace Organic Acids in Grape Juice
selectivity for carboxylic acids and alcohols. The weakly ion- Using The IonPac® ICE-AS6 Column
ized acids are separated by pKa differences, size, and hydropho-
bicity. Strong acid anions such as chloride, oxalate, and sulfate 15
Column: IonPac ICE-AS6
elute in the void and do not interfere with the quantification of Eluent: 0.4 mM Heptafluorobutyric acid
2
the organic acids. Flow Rate:
Inj. Volume:
1.0 mL/min
50 µL
5
Detection: Suppressed conductivity, AMMS® ICE
µS Peaks: 1. Inorganics
The ICE-AS6 column can be used with any typical strong acid 2. Tartaric
3. Galacturonic
eluent and a wide range of detectors, including conductivity, 3
4. Citric
IC & RFIC Columns
amperometry, photometry, and refractive index to determine 1 4

5. Malic
6 7 8 6. Formic
aliphatic organic acids. Use the AS11 for fast analysis of organic 7. Acetic
0 8. Unknown
acids and inorganic anions in well-characterized samples. Use 0 5 10 15 20
Minutes
the high-capacity AS11-HC column for organic acids and
14430
inorganic anions in complex sample matrices or uncharacterized
samples. Determination of trace organic acids in grape juice using suppressed
conductivity detection.
Note: Use the Anion MicroMembrane Ion-Exclusion
Suppressor (AMMS ICE 300) with the ICE-AS6 column.
222 www.dionex.com
IonPac ICE-AS6
Technical Notes
Determination of Organic Acids in Sulfuric Acid
Using the IonPac® ICE-AS6 Column TN 44: The Determination of Trace Anions in Concentrated
Phosphoric Acid
1.0
Eluent: 1.0 mM Heptafluorobutyric acid TN 46: Determination of Trace Anions in Concentrated Gly-
Flow Rate:
Detection:
1.0 mL/min
Suppressed conductivity,
colic Acid
AMMS® ICE
µS Sample: 0.25% Sulfuric acid, 50 mL
Peaks: 1. Citric
1
2
3 4 5 6 2. Malic Ordering Information
3. Glycolic
0 4. Lactic
5. Acetic
0 5 10 15 20 6. Succinic IonPac ICE-AS6
Minutes
IonPac ICE-AS6 Analytical Column (9 × 250 mm)........................................ 046023
14428-01
Ion exclusion allows the determination of organic acids in sulfuric acid

and eliminates sulfate interference.
Determination of Strongly Ionized Organic Acids

Using the IonPac ICE-AS6 Column
0.3
3
Eluent: 0.01 M Sulfuric acid
1 4 5
AU
2 Peaks: 1. Glucuronic
2. Galacturonic
3. Tartaric
IC & RFIC Columns

4. Citric
5. Malonic
0
0 2 4 6 8 10 12 14
Minutes
14431
Determination of strongly ionized organic acids using UV detection.
Related Literature
Product Data Sheets
IonPac ICE-AS6 Ion-Exclusion Column Data Sheet
Application Notes
AN 46: Ion Chromatography: A Versatile Technique for the

Analysis of Beer
AN 104: Analysis of Personal Care Products by Ion Chromatog-

raphy
www.dionex.com 223
IonPac ICE-Borate Use of Trace-Borate Analysis System for
Ion-exclusion column ideal for monitoring trace Monitoring Breakdown of Deionization Cartridges
levels of borate in high-purity water 2
(A) Before Separator
µS Breakthrough Column: IonPac® ICE-Borate
Concentrator
0
The IonPac ICE-Borate ion-exclusion column is designed for Column: IonPac TBC-1 Concentrator
Eluent: 2.5 mM MSA, 60 mM mannitol
monitoring trace levels of borate in high-purity water. Flow Rate: 1 mL/min
0 2 4 6 8 10 12 AMMS® ICE II
Minutes Regenerant: 25 mM TMAOH
Used with the TBC-1 Borate Concentrator column and conduc- 2 15 mM mannitol
(B) After Regenerant
tivity detection, the ICE-Borate ion-exclusion column supports µS Breakthrough
1
Flow Rate: 3 mL/min
the determination of borate at ng/L (ppt) concentrations. Borate 0
Sample Pump
Flow Rate: 8 mL/min
is separated and detected as the mannitol complex. Use the Sample Volume: 160 mL
Peaks: 1. Boron 700 ng/L (ppt)
Anion MicroMembrane Ion-Exclusion Suppressor (AMMS-ICE
0 2 4 6 8 10 12
300) with the ICE-Borate column. Minutes
13809
IC preconcentration system for trace-level borate determination.

Preconcentration of ng/L Levels of Borate
Using the IonPac® TBC-1 Column
Column: IonPac ICE-Borate

Related Literature
1 Concentrator
Column: IonPac TBC-1 Borate
Eluent: 2.5 mM MSA For detailed specifications and applications, see the following
60 mM mannitol
µS
Flow Rate: 1 mL/min
Detection Suppressed conductivity,
A AMMS® ICE II
0 Regenerant: 25 mM TMAOH
1 B 15 mM Mannitol Product Data Sheets
Sample
concentrated: 160 mL at 8 mL/min
0 5 10 Matrix: 5 ppb carbonate IonPac TBC-1 Borate Concentrator Column and IonPac ICE-
Minutes Trace A:
Trace B:
House deionized water
Borate (from boric acid)
Borate Column Data Sheet
as boron, 100 ng/L (ppt)
IC & RFIC Columns
Peaks: 1. Borate
13533
Determination of ng/L concentrations of borate using sample precon-
centration. IonPac ICE-Borate
IonPac ICE-Borate Analytical Column (9 × 250 mm).................................... 053945
Preconcentration System for Trace Borate Analysis
Waste Eluent In
6 1 2
Trace
5 3
Borate 4
Concentrator
(TBC-1)
Analytical
Column
Sample (ICE-Borate)
Pump
Conductivity Cell
AMMS-ICE II
Regenerant In
Waste
13778
IC preconcentration system for trace-level borate determination.
224 www.dionex.com
IC Trap Columns
Columns to purify eluent or polish samples
Trap columns are short columns installed in the system to
prevent unwanted analytes from interfering with the separa-
tion of your analytes of interest. IonPac trap columns contain
high-capacity, low-efficiency, ion-exchange resin. The columns
strip trace contaminants from the eluent and prevent them from
reaching the guard and analytical columns. Polisher columns are
specialized columns that remove unwanted counterions from the
sample, and are installed between the sampler and injector. Anion Trap Columns: Anion trap columns prevent anionic con-
taminants from causing spurious peaks
Cation Trap Columns: Cation trap columns prevent cationic
contaminants from causing spurious peaks
Cation Polisher: For removal of metallic contaminants and
other cations such as calcium and magnesium from the sample
stream during anion analysis.
MFC-1 Metal-Free: Designed to remove transition metals from
high-pH eluents.
IC & RFIC Columns
www.dionex.com 225
IC Trap Columns
Anion Trap Columns ATC-HC Borate Form Anion Trap
Column
IonPac Anion Trap Columns contain high-capacity, anion-
exchange resin in the hydroxide form. The anion trap column is The ATC-HC Borate Form Anion Trap Column is a high capac-
installed in the eluent line prior to the injection valve to prevent ity anion-exchange trap column designed for use with borate
eluent contaminants from causing spurious peaks during gradi- eluents.
ent chromatography.
The ATC-HC Borate Form is placed between the pump out-
let and the inlet of the EGC-KOH II cartridge to strip anionic
Related Literature contaminants from the deionized water and prevent them from
reaching the guard and analytical columns.
Product Data Sheets
Accessories
Concentrator and Trap Columns Data Sheet
Trap Column / Suppressor Cleanup Kit......................................................... 059659
ATC-HC Anion Trap Column - Borate Form (9 × 75 mm)............................... 064755
ATC-3 Anion Trap Column
The IonPac ATC-3 Anion Trap Column contains a high-capac-
ity, low-efficiency, anion-exchange resin used to remove trace
anion contamination from eluents.
IC & RFIC Columns
Accessories
ATC-3 4 mm Anion Trap Column (9 × 24 mm)............................................... 059660
ATC-3 2 mm Anion Trap Column (4 × 35 mm)............................................... 059661
ATC-HC Anion Trap Column

The IonPac ATC-HC Anion Trap Column is a high-capacity,
anion-exchange trap column designed for use with hydroxide
eluents.
For RFIC, EGC-KOH applications, use the CR-ATC.
Accessories
ATC-HC Anion Trap Column (9 × 75 mm)...................................................... 059604
226 www.dionex.com
Cation Trap Columns
Cation Trap Columns Cation Polisher
IonPac Cation Trap Columns contain high-capacity, cation-ex- IonPac CP1 Na+ Form and CP2 H+ Form Cation Polishers are
change resin in the hydronium form. The cation trap column is designed for removal of metallic contaminants and other cations
installed in the eluent line prior to the injection valve to prevent such as calcium and magnesium from the sample stream during
eluent contaminants from causing spurious peaks during gradi- anion analysis. They prevent high levels of metals or cations in
ent chromatography. samples from collecting on columns or suppressors, which can
cause performance issues such as poor peak shapes or anion
recoveries.
Related Literature
• Helps extend column and suppressor lifetimes
For detailed specifications and applications, see the following • Suited to phosphate analysis with metallic contaminants
• CP1 Na+ form: specifically designed for autosampler
operation
Product Data Sheets
• CP2 H+ form: recommended for large-volume sample
Concentrator and Trap Columns Data Sheet preconcentration applications using an external pump
Dionex Cation Polisher Columns can aid removal of matrix

CTC Cation Trap
cations during anion analysis with sample preconcentration.
The IonPac CTC Cation Trap Column contains high-capacity, Matrix cations can elute species of interest from the concentra-
low-efficiency, cation-exchange resin in the hydronium ion tor column leading to poor peak shapes and recovery. Removing
form, to remove trace cation contaminants from eluents. The the matrix cations using the Cation Polisher Column enables
trap is installed in the eluent line prior to the injection valve to improved chromatographic performance.
prevent spurious peaks and reduce baseline shifts during gradi-
ent chromatography. For RFIC-EGC MSA applications, use the
CR-CTC. Related Literature
IC & RFIC Columns

Ordering Information PDF documents under Literature on www.dionex.com.
Accessories Product Data Sheets

IonPac CTC-1 Cation Trap Column................................................................ 040192 Concentrator and Trap Columns Data Sheet
IonPac CTC Cation Trap Column 2 mm......................................................... 043132
Cation Polisher Na+ for Anion Analysis

The CP1 Na+ Form (6 × 16 mm) column is a cation exchange
column in the sodium form, packed in a low pressure format.
It is specifically designed for autosampler operation. The void
volume of this column is approximately 250 µL.
Cation Polisher Na+

IonPac CP1 Na+ Form Cation Polisher Column (6 × 16 mm)........................ 064930
www.dionex.com 227
IC Trap Columns
Cation Polisher H+ for Anion Analysis MFC-1 Metal-Free
The IonPac MFC-1 metal-free column contains a special chelat-
Simulated Borated Water Sample Before and After Polishing
30
ing resin that strips trace transition metal contaminants from
Without polishing sample Column: IonPac® AG4A, AS4A, 4mm high-pH eluents.
Eluent: EG Gradient with 100 mM Boric acid
µS Time (min) Concentration (KOH, mM)
0 5.0
3
1 2 10
10.1 65
5.0
Related Literature
25 65
0
30 Suppressor: ASRS-ULTRA® II, 4 mm, 300 mA
After polishing sample with CP2 Conc. Column: IonPac AG4A For detailed specifications and applications, see the following
1
Volume: 40 mL
Sample: Concentration (µg/L)
µS 1. Fluoride 10
2 Carbonate —
3
2. Chloride 10
3. Sulfate 10
Product Data Sheets
Boron 1500 (mg/L)
0 Li 3.5
0 6.0 12.0 18.0 25.1 Concentrator and Trap Columns Data Sheet
Minutes 23323
MFC-1 Metal Free Column

The CP2 H+ Form (9 × 24 mm) column is a higher-capacity
version in the hydronium form, with a void volume of approxi- The IonPac MFC-1 metal-free column is installed in the eluent
mately 825 µL. The CP2 is recommended for large-volume line prior to the injection valve to clear trace transition metal
sample preconcentration applications using an external pump. contaminants from high-pH eluents.
The Cation Polisher Columns can be regenerated off-line
approximately every 2-3 months (depending on the level of
contamination and usage). Ordering Information
Ordering Information Accessories

IC & RFIC Columns
MFC-1 Metal-Free Column (3 × 27 mm)....................................................... 037017

Cation Polisher H+
IonPac CP2 H+ Form Cation Polisher Column (9 × 24 mm).......................... 064931
228 www.dionex.com
IC Concentrator Columns
Columns to capture and concentrate sample ions
from large injection volumes
IonPac concentrator columns are designed primarily for high
purity water analysis. A concentrator column retains ions from
a measured volume of aqueous sample matrix, concentrating
the analyte species and lowering detection limits. Concentra-
tor columns typically have internal volumes of hundreds of
microliters, but can concentrate the ions from tens of milliliters,
increasing sensitivity by 2–5 orders of magnitude compared to Anion Concentrator Columns: Retains anions from a measured
standard sample loops. volume of sample matrix, concentrating the analyte species.
Cation Concentrator Columns: Retains cations from a measured
volume of aqueous sample matrix, concentrating the analyte
species.
Transition Metal: High-capacity, cation-concentrator column
for coupling the MetPac CC-1 chelating column to the IonPac
CS5A analytical column.
IC & RFIC Columns
www.dionex.com 229
Anion Concentrator Columns Ordering Information
IonPac anion concentrator columns are designed primarily for UTAC-LP1

high-purity water analysis. The concentrator retains ions from a
measured volume of aqueous sample matrix, concentrating the UTAC-LP1 Ultra Trace Anion Concentrator—Low Pressure (4 × 35 mm).... 063079
analyte species and lowering detection limits by 2–5 orders of
magnitude.
UTAC-ULP1
The UTAC-ULP1 Ultra Trace Anion Concentrator Column is a
Related Literature pellicular anion-exchange concentrator column with low void
volume of approximately 145 µL.
PDF documents under Literature on www.dionex.com. • Ultraclean (low sulfate) concentrator column
• Supports carbonate/bicarbonate, borate, and hydroxide
Product Data Sheets eluents
The UTAC-ULP1 is a general purpose concentrator for use with
syringe or autosampler loading. This ultralow backpressure
UTAC-LP1 concentrator can be used with the AS40, AS50, or AS Autosam-
plers, and with single piston sample delivery pumps including
the DXP-1 and DQP. It is compatible with carbonate/bicarbon-
Manual Concentration on UTAC-LP1 vs. Direct Injection ate, borate, and hydroxide eluents.
2 mL Injection on UTAC-LP1 Column: IonPac® AG18, AS18, 4 mm
7.00 Eluent: 23 mM Potassium hydroxide
5
Eluent Source: EGC II KOH cartridge with CR-ATC
6 9 Flow Rate: 1.0 mL/min Key Specifications
2 8 10 Inj. Volume: See chromatograms
µS 4 11 Temperature: 30° C
13 7 Detection: Suppressed conductivity, Format: 5 × 23 mm
ASRS® ULTRA II, 4 mm,
IC & RFIC Columns
–1.00 AutoSuppression® recycle mode

Capacity: 25.0 µeq/column
25 µL Direct Injection
0.120 2. Acetate 0.25
5
2 6
7 9 3. Formate 0.01 Void Volume: approximately 145 µL
8 10 4. Chlorite 0.05
4 11 5. Chloride 0.03
µS 13 Backpressure: <30 psi at 2.0 mL/min.
6. Nitrite 0.05
7. Carbonate 0.20
8. Bromide 0.10
–0.050 9. Sulfate 0.10
0 5
Minutes
10 14 10. Nitrate 0.10 Ordering Information
11. Chlorate 0.10
21572
UTAC-ULP1
The UTAC-LP1 Ultra Trace Anion Concentrator Column is a
UTAC-ULP1 Ultra Trace Anion Concentrator—Ultralow Pressure
pellicular anion-exchange concentrator with low void volume. (5 × 23 mm).................................................................................................... 063475
The ultraclean (low sulfate) UTAC-LP1 is a general purpose
concentrator column for use with syringe or autosampler load-
ing. UTAC-XLP1
• Low-backpressure column The UTAC-XLP1 Ultra Trace Anion Concentrator Column is a
pellicular anion-exchange concentrator column with low void
• Compatible with the AS40 Autosampler
volume (approximately 145 µL).
eluents • Ultraclean (low sulfate) concentrator column
Key Specifications eluents
Format: 4 × 35 mm • Supports carbonate/bicarbonate, borate, and hydroxide

eluents
Capaticy: 25.0 µeq/column
Void Volume: approximately 145 µL
Backpressure: <60 psi at 2.0 mL/min.
230 www.dionex.com
Anion Concentrator Columns
The UTAC-XLP1 is a general purpose concentrator for use with Key Specifications
syringe or autosampler loading. This low-backpressure column
Format: 4 × 35 mm
can be used with the AS40, AS50, or AS Autosamplers, and
with single piston sample delivery pumps including the DXP-1 Capacity: 25 µeq/column
and DQP.
Key Specifications Backpressure: <60 psi at 2.0 mL/min.
Format: 6 x 16 mm
Void Volume: approximately 145 µL. TAC-LP1
IonPac TAC-LP1 Low-Pressure Anion Concentrator (4 × 35 mm)................. 046026
Backpressure: <10 psi at 2.0 mL/min.
Ordering Information TAC-ULP1
UTAC-XLP1 Determination of Trace Anions Using the

IonPac® TAC-ULP1 Concentrator Column
UTAC-XLP1 Ultra Trace Anion Concentrator—Extremely Low Pressure Conc. Column: IonPac TAC-ULP1 (5 × 23 mm)
(6 × 16 mm).................................................................................................... 063459 Column: IonPac AG18, AS18 4 mm
Eluent: 23 mM Potassium hydroxide
3.5
Source: EGC-KOH
TAC-LP1 6 10 Inj. Volume:
Detection:
1 mL
Suppressed conductivity, ASRS® ULTRA,
8
µS 4 mm, AutoSuppression® recycle mode
2 4 11 Peaks: 1. Fluoride 0.01 mg/L (ppm)
3 7
Preconcentration of Trace Anions 1 2. Acetate 0.05
3. Formate 0.02
Using the TAC-LP1 Concentrator Column 4. Chlorite 0.10
IC & RFIC Columns

5. Chloride 0.06
–0.5 6. Nitrite 0.10
Column: IonPac® AG14, AS14, 4 mm 0 5 10 15 7. Carbonate 0.40
10 Concentrator Minutes 8. Bromide 0.20
Column: TAC-LP1 9. Sulfate 0.20
Eluent: 3.5 mM Sodium carbonate/ 10. Nitrate 0.20
1.0 mM Sodium bicarbonate 11. Chlorate 0.20 19324
µS
1 Inj. Volume: 5 mL
3 4 Detection: Suppressed conductivity,
2
ASRS® ULTRA, 4 mm,
0
AutoSuppression® The IonPac TAC-ULP1 Trace Anion Concentrator Column is
external water mode a pellicular anion-exchange concentrator with moderately low
Peaks: 1. Chloride 9.7 µg/L (ppb)
2. Unidentified - void volume. It is an ultralow pressure concentrator for use with
0 2 4 6 8 10 12 14
Minutes ISO calibration data unavailable syringe or autosampler loading (AS40 and AS50 Autosamplers).
It can be used with single-piston sample delivery pumps (e.g.,
13934 DXP-1 or DQP). Compatible with carbonate, borate, or hydrox-
ide eluents.
The IonPac TAC-LP1 Trace Anion Concentrator Column is a
pellicular anion-exchange concentrator. Key Specifications
• Supports carbonate/bicarbonate, borate, and hydroxide Format: 5 × 23 mm

eluents
The TAC-LP1 is a general purpose, low-pressure concentrator Void Volume: approximately 145 µL
for use with syringe or autosampler loading with void volume of
approximately 145 µL. The TAC-LP1 is also designed for use as Backpressure: <30 psi at 2.0 mL/min
the concentrator column in the SP10 AutoNeutralization module
for anions.
TAC-ULP1
TAC-ULP1 Ultralow Pressure Trace Anion Concentrator (5 × 23 mm)......... 061400
www.dionex.com 231
TAC-2 Ordering Information
The IonPac TAC-2 Trace Anion Concentrator Column is a pel-

AMC-1
licular anion-exchange concentrator column with moderately
low void volume (~50 µL). IonPac AMC-1 Anion Micro Concentrator (2 × 15 mm)............................... 051760
• Can be used with carbonate/bicarbonate and borate eluents AC10

Note: Not solvent compatible. The IonPac AC10 is an anion-exchange concentrator column
designed for use with the IonPac AS10 column.
Key Specifications
TAC-2 Format: 4 × 50 and 2 × 50 mm
IonPac TAC-2 Trace Anion Concentrator....................................................... 043101 Capacity: 4.0 µeq/column (4 mm); 0.8 µeq/column (2 mm)
IonPac TAC-2 Trace Anion Concentrator, Pkg of 4........................................ 043102
Void volume: 207 µL (4 mm); 52 µL (2 mm)
Backpressure: <300 psi at 1.0 mL/min (4 mm);
AMC-1
<300 psi at 0.25 mL/min (2 mm)
Determination of Trace-Level Fluoride, Chloride, and Sulfate Ordering Information

Standard Using an AMC-1 Anion Microconcentrator Column
and the AS12A Column
Column: IonPac® AG12A, AS12A, 2 mm
AC10
2 Concentrator
Column: AMC-1 IonPac AC10 Concentrator Column (4 × 50 mm).......................................... 043133
1 Eluent: 2.1 mM sodium carbonate
0.8 mM sodium bicarbonate IonPac AC10 Concentrator Column (2 × 50 mm).......................................... 043134
Inj. Volume: 36 mL
IC & RFIC Columns

3
ASRS® ULTRA, 2 mm,
6
AutoSuppression® external water mode AC15
Peaks: 1. Fluoride 0.36 µg/L (ppb)
4 5 2. Carbonate -
3. Chloride 0.34 Determination of Inorganic Anions and Low-Molecular-Weight
0 4. Unidentified -
0 2 4 6 8 10 12 14 16 18 20 5. Unidentified - Organic Acids with Sample Preconcentration
Minutes 6. Sulfate 0.70 Using the IonPac® AS15 Column
13138
4.0 6
Concentrator
7 Column: IonPac AC15
The AMC-1 is a very low void volume microconcentrator µS
4 8 Eluent: Potassium hydroxide,
1
column designed for the concentration of inorganic anions and 23
9
10
11 10 mM from 0 to 4 min,
10–40 mM from 4 min to 14 min,
5
low-molecular-weight organic anions from ultrapure water. A 0 40–60 mM from 14 to 18 min
Eluent Source: EG40
unique, solvent-compatible resin technology ensures a low sul- 0 5 10 15 20 Temperature: 30 °C
Minutes Flow Rate: 0.5 mL/min
fate background and ruggedness during the concentration step. Sample Vol.: 20 mL, preconcentrated
Peaks: 6. Carbonate –
1.Fluoride 0.1 µg/L (ppb) 7. Sulfate 0.1
ASRS® ULTRA, 2 mm,
2. Acetate 0.1 8. Oxalate 0.1
The AMC-1 can be loaded with either a loop or sample load- 3. Formate 0.1 9. Bromide 0.1
4. Chloride 0.1 10. Nitrate 0.1
ing pump. The low void volume (approximately 15 µL) ensures 5. Nitrite 0.1 11. Phosphate 0.1
14871
accurate determination of early-eluting anions such as fluoride,
glycolate, acetate, and formate. The AMC-1 can be used with
The IonPac AC15 is an anion-exchange concentrator column
carbonate/bicarbonate or borate eluents.
designed for use with the IonPac AS15 column. Use the AC15
2 × 50 mm format for 2 and 3 mm applications.
Key Specifications
Key Specifications
Format: 2 × 15 mm
Format: 4 × 50 and 2 × 50 mm
Capacity: 3 µeq/column
Capacity: 9 µeq/column (4 mm); 2.2 µeq/column (2 mm)
Void volume: 15 µL
Void volume: 150 µL (4 mm); 53 µL (2 mm)
Backpressure: 60–110 psi at 0.5 mL/min
Backpressure: <140 psi at 1.0 mL/min; <160 psi at 0.42 mL/min
232 www.dionex.com
Anion Concentrator Columns
Ordering Information with highly selective polyol groups. The TBC-1 is coupled to a
specially designed ICE-Borate column for the determination of
borate at ng/L (ppt) concentrations.
AC15
IonPac AC15 Concentrator Column (4 × 50 mm).......................................... 055694 Mannitol eluent quantitatively elutes borate from the TBC-1
IonPac AC15 Concentrator Column (2 × 50 mm).......................................... 055695 concentrator column.
Cryptand C1 Ordering Information
The IonPac Cryptand C1 Concentrator Column is recommended

for the analysis of trace perchlorate in drinking water. This TBC-1
adjustable-capacity column contains a macroporous, 17.5 µm IonPac TBC-1 Trace Borate Concentrator (4 × 50 mm)................................ 053944
resin grafted with the macrocyclic 2,2,2 cryptand compound.
The column’s functional capacity depends on eluent concentra-
tion and the type of cation bound within the cryptand molecule.
• Approximately 30 µeq/col of cryptand capacity available

for use
• Capacity adjustable from fixed amount to zero by via eluent
concentration or cation type
The adjustable capacity makes the Cryptand C1 a powerful tool

for determining trace perchlorate in drinking water and high
ionic strength water. Methods using the C1 can quantify 140
ng/L of perchlorate in a background of total dissolved solids
with concentrations as high as 3000 mg/L. The Cryptand C1 is
specified for sample preconcentration in US EPA Method 314.1.
IC & RFIC Columns

Cryptand C1
IonPac Cryptand C1 Concentrator Column (4 × 35 mm)............................... 062893
IonPac TBC-1
Preconcentration of ng/L Levels of Borate

Using the IonPac® TBC-1 Column
Column: IonPac ICE-Borate

1 Concentrator
Column: IonPac TBC-1 Borate
Eluent: 2.5 mM MSA, 60 mM mannitol
µS Flow Rate: 1 mL/min
Detection Suppressed conductivity,
AMMS® ICE II
A Regenerant: 25 mM TMAOH, 15 mM Mannitol
0
1 Sample
B
concentrated: 160 mL at 8 mL/min
Matrix: 5 ppb carbonate
Trace A: House deionized water
0 5 10 Trace B: Borate (from boric acid)
Minutes as boron, 100 ng/L (ppt)
Peaks: 1. Borate
13533
The IonPac TBC-1 Trace Borate Concentrator Column is

optimized for trace analysis of borate. The TBC-1 has a low
void volume and is packed with a unique resin surface grafted
www.dionex.com 233
Cation Concentrator Columns TCC-LP1
IonPac cation concentrator columns are designed primarily for

Column Switching for Trace Cations in Ethanolamine-Treated Water
high-purity water analysis. The concentrator strips ions from a
measured volume of aqueous sample matrix, concentrating the
Columns: IonPac® CG12A, CS12A, 2 mm
analyte species and lowering detection limits by 2–5 orders of 5 6
CG11, CS11, 2 mm
10 Trap Column: CTC-1
magnitude. Concentrator: TCC-LP1, 4 mm
Eluent: 22.5 mM methanesulfonic acid
Sample Vol.: 14.5 mL
µS Detection : Suppressed conductivity,
TCC-2 7
CSRS® ULTRA, 4 mm,
1 4
2 3 Peaks: 1. Magnesium 0.6 µg/L (ppb)
0 2. Calcium 1.2
Preconcentration of ng/L Levels of Transition Metals 3. Lithium 0.15
4. Sodium 0.3
Using the IonPac® CS5A Column 0 10 20 30 40
5. Ammonium 1000
Minutes
6. Ethanolamine 5000
Columns: IonPac CG5A, CS5A, 2 mm 7. Potassium 3.0
2.50 × 10–2 Concentrator Column: IonPac TCC-2
4 Eluent: MetPac™ PDCA
Flow Rate: 0.3 mL/min 11543
1
Postcolumn
2 Reagent: PAR in MetPac Postcolumn
7 8
5 Reagent Diluent
AU
3 6 PCR Flow Rate: 0.15 mL/min The IonPac TCC-LP1 Trace Cation Concentrator Column is a
Detection: 530 nm, 10 mm cell
Temp: 30 °C pellicular cation-exchange concentrator with moderately low
Preconcentrated Vol.: 4.0 mL
void volume (approximately 145 µL).
Peaks: 1. Iron 500 ng/L (ppt)
1.00 × 10–1 2. Copper 250
0 2 4 6 8 10 12 14 16 3. Nickel 500
Minutes 4. Zinc >500 The TCC-LP1 is a general purpose, very low-pressure con-
5. Cobalt 250
Note: Zn contamination present.
6. Cadmium 1250 centrator for use with syringe or autosampler loading. It is
7. Manganese 750
8. Iron 500
also designed for use as the concentrator column in the SP10
13774
AutoNeutralization module for cations. The TCC-LP1 can be
used with sulfuric acid, methanesulfonic acid, and hydrochloric
The IonPac TCC-2 Trace Cation Concentrator Column is a pel- acid eluents.
IC & RFIC Columns
licular cation-exchange concentrator with moderately low void

volume (approximately 50 µL). Note: The TCC-LP1 is recommended for use with
carboxylated columns such as the CS12, CS12A, CS14, CS15,
The TCC-2 is a surface-sulfonated, cation-exchange concentra- CS16, CS17, and CS18.
tor column that is ideal for use with sulfonated columns such as
the CS3, CS10, and CS11. It can also be used as a concentra-
Key Specifications
tor column for transition metals. The TCC-2 can be used with
hydrochloric acid or diaminopropionic acid eluent. Format: 4 × 35 mm
Capacity: 260 mequiv/column
Key Specifications
Void volume: 145 µL
Format: 3 × 35 mm
Backpressure: 70 psi at 1.0 mL/min
Void volume: 50 µL Ordering Information
Ordering Information Accessories

IonPac TCC-LP1 Low-Pressure Cation Concentrator.................................... 046027
Accessories
IonPac TCC-2 Trace Cation Concentrator (3 × 35 mm)................................. 043103
IonPac TCC-2 Trace Cation Concentrator, Pkg of 4....................................... 043104
234 www.dionex.com
Cation Concentrator Columns
TCC-ULP1 Key Specifications
Format: 6 × 16 mm
The IonPac TCC-ULP1 Trace Cation Concentrator Column is
a pellicular cation-exchange concentrator column with moder- Capacity: 260 µeq/column
ately low void volume (approximately 145 µL). It is designed
primarily for high purity water analysis. The column strips ions Void volume: approximately 145 µL
from a measured volume of aqueous sample matrix, concentrat- Backpressure: 30 psi at 1.0 mL/min
ing the analyte species, thereby lowering detection limits.
The TCC-ULP1 Ultralow Pressure Trace Cation Concentrator

Column is a general purpose, ultralow-pressure concentrator for Ordering Information
use with syringe or autosampler loading (AS40, AS50, or AS
autosamplers) and with single-piston sample delivery pumps
Accessories
including the DXP-1 and DQP. The TCC-ULP1 can be used
with sulfuric acid, methanesulfonic acid, and hydrochloric acid TCC-XLP1 Extremely-Low Pressure Trace Cation Concentrator
eluents. (6 × 16 mm).................................................................................................... 063889
Note: The TCC-ULP1 is recommended for use with

carboxylated columns such as the IonPac CS12, CS12A, CS14,
CS15, CS16, CS17, and CS18 columns.
Key Specifications
Format: 5 × 23 mm
IC & RFIC Columns

Backpressure: 45 psi at 1.0 mL/min
Accessories
TCC-ULP1 Ultralow Pressure Trace Cation Concentrator (5 × 23 mm)........ 063783
TCC-XLP1
The IonPac TCC-XLP1 Extremely-Low Pressure Trace Cation
Concentrator Column is a pellicular cation-exchange concentra-
tor with moderately low void volume.
The IonPac TCC-XLP1 is compatible with a syringe or au-

tosampler (AS40, AS50 or AS Autosamplers) and with single-
piston sample delivery pumps including the DXP-1 and DQP.
It is recommended for use with carboxylated columns such as
the IonPac CS12, CS12A, CS14, CS15, CS16, CS17, and CS18.
The column is used with sulfuric acid, methanesulfonic acid,
and hydrochloric acid eluents.
www.dionex.com 235
Transition Metal Concentrator
Columns
Dionex provides a trace metal concentrator for use in chelation
ion chromatography.
Related Literature
Product Data Sheets
TMC-1
Determination of Transition Metals in Sea Water Using

Chelation Ion Chromatography and the IonPac® CS5A Column
0.02 Spiked Sea Water
4 Columns: IonPac CG5A, CS5A, 4 mm,
7 MetPac™ TMC-1
1 5 6
2 3 Eluent: PDCA, isocratic
AU
Preconc. Vol.: 2.5 mL
Detection: Visible, 530 nm
–0.01 Postcolumn
Reagent: PAR at 0.6 mL/min
IC & RFIC Columns
0.02 Sea Water Peaks: Sea Water Spike Amt. Spiked Sea Water
1. Iron 10.8 mg/L 10.0 mg/L 21.6 mg/L (ppb)
7 2. Copper — 2.50 2.42
1 4
AU 3. Nickel 1.60 5.00 5.85
3
4. Zinc 1.88 5.00 6.78
5. Cobalt — 2.50 2.57
6. Cadmium — 12.5 12.5
–0.01 7. Manganese 7.16 7.50 14.3
0 2 4 6 8 10 12 14
Minutes 12427
The IonPac TMC-1 Trace Metal Concentrator is a high-capacity

cation concentration column used for coupling the MetPac CC-1
to the IonPac CS5 and CS5A analytical columns when perform-
ing chelation IC.
Key Specifications
Format: 3 × 25 mm
TMC-1
IonPac TMC-1 Trace Metal Concentrator (3 × 25 mm)................................. 049000
236 www.dionex.com
Bio Columns
Protein & Peptide Columns 239 Nucleic Acid Columns 261
ProPac SCX and WCX......................................... 240 DNAPac PA100.................................................... 262

Related Literature.............................................................. 241 Separates RNA / Double-Stranded DNA..................... 262
ProPac SAX and WAX........................................ 242
Related Literature.............................................................. 243 DNAPac PA200.................................................... 263
ProPac PA1........................................................... 244
Amino Acid Columns 265
ProPac HIC........................................................... 245
AminoPac PA1..................................................... 266
ProPac IMAC........................................................ 246 AminoPac PA10................................................... 267

ProSwift RP.......................................................... 248

Carbohydrate Columns 269
Bio Columns
CarboPac MA1..................................................... 270
ProSwift IEX......................................................... 250
CarboPac PA1...................................................... 272
Acclaim 300 C18.................................................. 252
CarboPac PA10.................................................... 274
Strong Cation Exchange..................................... 254
Acclaim PepMap................................................. 255
CarboPac PA20.................................................... 276
Acclaim PepMap100 C18................................................. 255
Acclaim PepMap300 C18................................................. 256
Acclaim PepMap100 C8................................................... 256
CarboPac PA100.................................................. 278
Acclaim PepMap300 C4................................................... 256
Acclaim PepMap µ-Precolumns.................................... 257 Related Literature.............................................................. 279
Acclaim PepMap µ-Guard Columns.............................. 257 Ordering Information........................................................ 279
Acclaim PepMap100 C18 Nano-Trap Columns........... 257
Titanium-Dioxide Nano-Trap Columns.......................... 258 CarboPac PA200.................................................. 280
PepSwift............................................................... 258 Ordering Information........................................................ 280
www.dionex.com 237

Bio Columns
238 www.dionex.com
Protein & Peptide Columns
HPLC analysis of proteins and peptides
The Bio column line offers a complete selection of ion-ex-
change and reversed-phase columns for the analysis and purifi-
cation of proteins and peptides, nucleic acids, amino acids, and
other biological molecules. Stationary phases include polymer
bead and polymer monolith ion-exchange phases, and polymer
and silica-based reversed-phase. Dionex also supports IMAC
and HIC applications with specialty columns.
ProPac SCX and WCX: Nonporous, strong and weak cation-ex-
• ProPac ion exchange for protein, glycoprotein analysis and change columns for the separation of proteins and their variants.
monoclonal antibody analysis.
ProPac SAX and WAX: Strong and weak anion exchangers with
• Acclaim PepMap reversed-phase columns for peptide quaternary ammonium and tertiary amine functional groups,
analysis respectively, attached to a nonporous core.
• ProSwift and PepSwift monoliths for fast high-resolution ProPac PA1: Strong anion-exchange column for the analysis of
separation and purification of proteins and peptides hydrophilic anionic proteins and peptides with pI values from 3
• Specialty columns for IMAC and HIC separations to 11; offers base stability.
ProPac HIC: Silica-based high-resolution, high-capacity HPLC
ProPac ion-exchange columns separate protein variants and
column for the separation of proteins by hydrophobic interac-
monoclonal antibodies, resolving isoforms that differ by as little
tion chromatography.
as one charged residue. Applications include analysis of blood
and dairy proteins. Acclaim silica-based columns provide both ProPac IMAC: High-resolution HPLC column for gradient
traditional and capillary formats for peptide mapping and high- separations in Immobilized Metal Affinity Chromatography
efficiency protein separations. mode.
ProSwift IEX: Unique, high-capacity monolith columns for
high-resolution, fast separations of proteins and other biomol-
ecules.
Bio Columns
ProSwift RP: Unique, reversed-phase monolith columns for
high-speed, high-resolution separations of proteins and other
biomolecules.
Acclaim 300 C18: Reversed-phase column for the separation of
peptides, proteins, and other biological macromolecules.
Acclaim Polysulfoethyl: Polysulfoethyl columns.
Acclaim PepMap: Silica-based reversed-phase columns for
superior protein and peptide separations.
PepSwift: Monolithic columns for fast analysis of peptides and
proteins using nano and capillary LC coupled to MS.
www.dionex.com 239
ProPac SCX and WCX Separation of Hemoglobin Variants
Strong and weak cation-exchange columns for the Using the ProPac® SCX-10 Column
high-resolution separation of proteins and MAbs Column: ProPac SCX-10, 4 × 250 mm
Eluent: A) 20 mM Sodium phosphate,
1 4 mM Potassium cyanide, pH 6
ProPac SCX-10 and WCX-10 strong and weak cation-exchange 1.60 B) 1 M Sodium chloride in water
C) Water
columns are based on a nonporous core particle that results in
2 Gradient: Time %A %B %C
high-efficiency separations of protein variants. A hydrophilic 3 Init 50 0 50
AU
layer prevents unwanted secondary interactions. A grafted 4 30 min 50 50 0
cation-exchange surface provides pH-based selectivity control Flow Rate: 1 mL/min

Inj. Vol.: 10 µL
and fast mass transfer for high-efficiency separation and moder- Detection: 220 nm
0
ate capacity. 0 5 10 15 20 25
Sample: 1. Fetal hemoglobin
2. Hemoglobin
Minutes 3. Sickle cell hemoglobin
4. Hemoglobin C
• Characterization and quality control assessment of mono-
14067
clonal antibodies and other proteins
• Unequalled resolution Separation of hemoglobin variants.
• High-efficiency separations with excellent sample recovery

Lysine truncation Column: ProPac WCX (4 × 250 mm)
variants*
• Unmatched column-to-column and lot-to-lot reproducibil- Eluents: (E1) 20 mM MES
+ 115 mM NaCl
ity + 1 mM EDTA, pH 5.5
(E2) 20 mM MES
K + 145 mM NaCl
• Useful for characterization of related protein variants (e.g., KK
+ 1 mM EDTA, pH 5.5
Gradient: t (min) %E1 %E2
deamidation and MAb lysine truncation variants) 0 100 0
AU 2 100 0
MAb sample as received 40 0 100
• Highly-efficient peaks with excellent sample recovery 60 0 100
Sample: MAb PDL
• Available in different dimensions and formats for flexibility Acidic charge
λ: 280 nm
Basic charge * Peak assignment supported by
in method development variants variants data from R.J. Harris, et.al,
J.Chromatogr., A 1995,705, 129–134.
and Carboxypeptidase B digest.
The ProPac SCX-10 is a strong cation exchange column with
sulfonate functional groups. The ProPac WCX-10 has a car- After digestion with carboxypeptidase B
boxylate functional group. Both are based on 10 µm nonporous, 0 10 20

Minutes
30 40 21104
polymeric beads and coated with a proprietary hydrophilic layer.
Bio Columns
MAb separation on ProPac WCS: characterization of lysine truncation

variants.
All ProPac columns are manufactured and tested under the
strictest specifications, resulting in unmatched column-to-
column and lot-to-lot reproducibility. Dionex also offers ProPac
columns as a lot select package: either three columns from one
lot or three columns from three different lots, to assist quality
control scientists in method validation.
240 www.dionex.com
ProPac SCX and WCX
Separation of Ribonuclease A and Its Two

Deamidation Products
SCX Analytical Columns
2
3 1
®
Column: ProPac WCX-10, 4 × 250 mm ProPac SCX-10 Analytical Column (4 × 250 mm)......................................... 054995
Eluent: A) 10 mM Sodium phosphate, pH 6.0
200 h @ 37° C
B) 10 mM Sodium phosphate,
1 1 M sodium chloride, pH 6.0
ProPac SCX-10 Analytical Column (2 × 250 mm)......................................... 063456
23
100 h @ 37° C
Gradient: Time %A %B
ProPac SCX-10 Semipreparative Column (9 × 250 mm)............................... 063700
AU 1
Init 9 4
23 30 min 30 70
ProPac SCX-10 Semipreparative Column (22 × 250 mm).............................SP5522
50 h @ 37° C
ProPac SCX-10 Lot Select Column Set (4 × 250 mm) (1 lot of resin)...........SP5727
Inj. Volume: 10 µL
Untreated (0 h)
Detection: Absorbance, 280 nm
ProPac SCX-10 Lot Select Column Set (4 × 250 mm) (3 lots of resin).........SP5728
0 5 10 15 20 25 30 Peaks: 1. Ribonuclease A
Minutes 2, 3. Deamidation products
SCX Guard Columns
14065
ProPac SCX-10G Guard Column (4 × 50 mm)............................................... 054996
Separation of ribonuclease A and its two deamidation products during ProPac SCX-10G Guard Column (2 × 50 mm)............................................... 063462
the course of forced deamidation.
WCX Analytical Columns
MAb Separation on ProPac WCX ProPac WCX-10 Analytical Column (2 × 250 mm)........................................ 063472
Effect of Salt Gradients (pH 5.5)
ProPac WCX-10 Analytical Column (4 × 100 mm)........................................SP5829
2.25 mM/min Column: ProPac WCX-10 (4 × 250 mm)
2.75 mM/min Eluent A: 20 mM MES
ProPac WCX-10 Analytical Column (4 × 250 mm)........................................ 054993
Eluent B: 20 mM MES + 250 mM NaCl
(NaCl Gradient 3.75 mM/min)
ProPac WCX-10 Lot Select 3 Column Set (4 × 250 mm) (1 lot of resin)......SP5512
3.25 mM/min Gradient: t (min) %A %B
0 95 5
ProPac WCX-10 Lot Select 3 Column Set (4 × 250 mm) (3 lots of resin).....SP5513
60 5 95
AU 3.75 mM/min
62 5 95 ProPac WCX-10 Semipreparative Column (9 × 250 mm)............................. 063474
62.5 95 5
Inj. Volume: 20 µL ProPac WCX-10 Semipreparative Column (22 × 250 mm)...........................SP5482
0.0600
4.25 mM/min Sample: 2.5 mg/mL MAb
Detection: 280 nm WCX Guard Columns
4.75 mM/min ProPac WCX-10G Guard Column (2 × 50 mm).............................................. 063480
-0.0100
0.0 Minutes 52.0 ProPac WCX-10G Guard Column (4 × 50 mm).............................................. 054994
23748
Bio Columns
MAb separation on ProPac WCX: effect of salt gradients at pH 5.5.
Related Literature
Product Data Sheets
ProPac Ion-Exchange Columns for Protein Analysis
www.dionex.com 241
ProPac SAX and WAX
Resolution of Phosphorylation Variants of Ovalbumin
High-resolution, strong and weak anion-exchange
columns for the separation of proteins
®
Column: ProPac SAX-10, 4 x 250 mm
Eluents: A) Water
The ProPac SAX-10 is a nonporous, strong anion-exchange B) 2.0 M NaCl
C) 0.1 M Tris/HCl (pH 8.5)
column (quaternary amine functional group). The ProPac WAX-
10 column is a weak anion exchanger (tertiary amine functional AU Gradient: Time
Init
%A
80
%B
0
%C
20
group). Both are based on a nonporous core particle that pro- 15 min 67.5 12.5 20
vides pressure stability. A hydrophilic layer prevents unwanted Ovalbumin + alk. phos.
Inj.Vol. 30 µL
secondary interactions and a grafted anion exchange surface Ovalbumin Detection: 214 nm
provides pH control, selectivity, and fast mass transfer for high 0 2 4 6 8 10 12 14 16
Sample: Ovalbumin before and after
alkaline phosphatase treatment
Minutes
efficiency separation, as well as moderate capacity.
16445
• Unequalled resolution
• High-efficiency peaks with excellent recovery Resolution of phosphorylation variants of ovalbumin.
• Higher capacity than other columns based on nonporous

particles Effect of Sialylation on Transferrin Chromatography
• Useful for characterization and quality control assessment Columns:

®
ProPac SAX-10, 4 × 250 mm
Eluents: A) Water
of closely-related protein variants B) 2.0 M NaCl
C) 0.2 M Tris/HCl (pH 9)
HOLO
• Supports separation of proteins that differ by as little as one Gradient: Time %A %B %C
amino acid residue HOLO + neur Init
30 min
87
83
3
7
10
10
AU
APO Lot #1
• Neutral hydrophilic coat that eliminates protein-resin hy- APO Lot #1+neur
Inj. Vol. 50 µL
drophobic interactions Detection: 214 nm
Samples: HOLO (iron rich) and APO (iron poor)
human transferrin samples before and
• Superior lot-to-lot and column-to-column reproducibility APO Lot #2 after neuraminidase treatment.
Digestions were carried out overnight
APO Lot #2 + neur
at 37 C in sodium acetate buffer
ProPac SAX-10 and WAX-10 columns can resolve protein 0 4 8 12 16 20 24 28 32 at pH 5.
Minutes
isoforms that differ by as little as one charged residue. Differ- 16446
ences in selectivity between the WAX-10 and SAX-10 provide

Bio Columns
flexibility in maximizing resolution of closely related species. Effect of sialylation on transferrin chromatography.
All ProPac columns are manufactured and tested under the

strictest specifications, resulting in unmatched column-to-
column and lot-to-lot reproducibility. Dionex also offers these
columns as a lot select package: three columns from one lot or
three columns from three different lots, to assist quality control
scientists in method validation.
242 www.dionex.com
ProPac SAX and WAX
50
α Column: ProPac SAX-10, 4 x 250 mm SAX Analytical Columns

Eluents: A) 4 M Urea, 0.01 M2-mercaptoethanol,
0.01 M HEPES, pH 7.3 ProPac SAX-10 Analytical Column (2 × 250 mm)......................................... 063448
B) 1.0 M NaC1, 4M Urea,
κ 0.01 M 2-mercaptoethanol, ProPac SAX-10 Analytical Column (4 × 250 mm)......................................... 054997
mAU 0.01 M HEPES, pH 7.3
Gradient: 3 min %B = 10 ProPac SAX-10 Lot Select Column Set (4 × 250 mm) (1 lot of resin)...........SP5729
30 min %B = 35
Flow Rates: 1.0 mL/min
Inj. Volume: 50 µg (mg/mL)
ProPac SAX-10 Lot Select Column Set (4 × 250 mm) (3 lots of resin).........SP5730
Detection: 280 nm
Samples: mixture of α, β & κ bovine caseins
ProPac SAX-10 SEmi-Preparative Column (9 × 250 mm)............................. 063703
β ProPac SAX-10 Semi-Preparative Column (22 × 250 mm)...........................SP5594
-5
0 5 10 15 20 25 30
Minutes
WAX Analytical Columns
24547
ProPac WAX-10 Analytical Column (2 × 250 mm)........................................ 063464
Profiling dairy milk caseins. ProPac WAX-10 Analytical Column (4 × 250 mm)........................................ 054999
ProPac WAX-10 Lot Select Column Set (4 × 250 mm) (1 lot of resin).........SP5731
ProPac WAX-10 Lot Select Column Set (4 × 250 mm) (3 lots of resin)........SP5732
High-Selectivity Anion-Exchange Column
ProPac WAX-10 Semi-Preparative Column (9 × 250 mm)............................ 063707
Column: ProPac® WAX-10, 4 x 250 mm

ProPac WAX-10 Semi-Preparative Column (22 × 250 mm)..........................SP5598
2.50 x 10–3
Eluent: 10 mM Tris (pH 8.0)
0.0–0.1 M NaCI; 0–30 min
Flow Rate: 1.0 mL/min WAX Guard Columns
Inj. Volume: 10 µL
Detection: 214 nm
Samples: Carbonic anhydrase ProPac WAX-10G Guard Column (2 × 50 mm).............................................. 063470
ProPac WAX-10G Guard Column (4 × 50 mm).............................................. 055150
0
SAX Guard Columns
ProPac SAX-10G Guard Column (2 × 50 mm)............................................... 063454
6 8 10 12 14
ProPac SAX-10G Guard Column (4 × 50 mm)............................................... 054998
Minutes
15712
Bio Columns
The ProPac WAX shows high selectivity for proteins in this separation
of carbonic anhydrase.
Related Literature
Product Data Sheets
www.dionex.com 243
ProPac PA1 Related Literature
For hydrophilic anionic protein separations For detailed specifications and applications, see the following
The ProPac PA1 column supports the analysis and purifica-
tion of hydrophilic anionic proteins and peptides. Proteins and
peptides that are highly cationic (e.g., those with very high Product Data Sheets
isoelectric points) may be resolved on this column, but some
may exhibit substantial binding to the column.
• Good for hydrophilic anionic proteins and peptides

• Ideal for high-resolution separations of proteins with pI
values from 3 to 11 Analytical Columns
• Available in semipreparative format ProPac PA1 Analytical Column (4 × 250 mm)............................................... 039658
• Pellicular packing ensures high-efficiency and fast mass ProPac PA1 Semipreparative Column (9 × 250 mm).................................... 040137
transport
Guard Columns
ProPac PA1 High Speed Column (4 × 50 mm)............................................... 039657
Fast Protein Standards Analysis in 120 Seconds
2 3,4 5
Column: ProPac® PA1 (4 × 50 mm)
Eluent: 10 to 350 mM NaCl
6 in 1.0 mM Tris, pH 8.2
Peaks: 1. Horseheart Myoglobin 33 µg
2. Contaminant –
3,4. Conalbumin 66
5. Ovalbumin 66
6. Soybean Trysin Inhibitor 66
0 50 100 150
Seconds
Bio Columns
3828
Gradient separation of protein standards.
Gradient Separation of Transferrin Glycoforms—

Rechromatography of Single Peak Derived by
Macroporous Anion Exchange
Column: ProPac® PA1 4-mm

Eluent: 20 mM Tris, pH 8.3, Isocratic,
Sodium chloride gradient
Sample: 50 mg purified Transferrin
(98% pure by FPLC)
0 5 10 15
Minutes
3275
Gradient separation of transferrin glycoforms.
244 www.dionex.com
ProPac HIC
ProPac HIC Separation of Snake Venom Proteins/Peptides
Hydrophobic Interaction Chromatography columns
for the separation of large biomolecules Column: 7.5 × 75 mm
(Competitors A and B)
7.8 × 75 mm
Hydrophobic Interaction Chromatography separates biomol- 100
1 100 (Dionex ProPac HIC-10)
Flow: 1.0 mL/min
3
ecules in a decreasing salt gradient, based on differences in 2
WVL: 214 nm
Eluents: (A) 2 M (NH4)2SO4 in
surface hydrophobicity. This method preserves the biological 0 4
5 0.1 M NaH2PO4 (pH 7.0)
(B) 0.1 M NaH2PO4 (pH 7.0)
activity of proteins. The HIC separation mechanism is comple- Dionex
Sample: Naja venom from Sigma
mentary to those of ion-exchange and gel filtration chromatog- mAU Competitor A
[1:1 diluted with 1 M (NH4)2SO4
in 0.1 M NaH2PO4 (pH 7.0)]
raphy. Inj. Volume: 20 µL
Competitor B
–200
• Based on 5 µm ultrahigh purity spherical silica gel par- 0 10 20 30 40 50 60 70
Minutes
ticles, with 300 Å pores
21289
• Proteins separated under non-denaturing conditions

Comparison of the separation of snake venom proteins/peptides using
• High protein loading capacity for protein purification ap- the ProPac HIC-10 and two competitors’ columns.
plications
• Proprietary multidentate silica bonding for enhanced hydro- Separation of Monoclonal Antibody (MAb)
lytic stability
1000
• High-resolution HPLC separation of proteins and peptides 10% CH3CN in eluent B
Column: Dionex ProPac® HIC-10, 4.6 × 100 mm
100 Eluents: (A) 0.5 M (NH4)2 SO4
• Wide range of applications in 0.1M NaH2PO4 (pH 7.0)
70 (B) 0.1 M NaH2PO4 (pH 7.0)
Gradient: 70–100% B in 15 min
The ProPac HIC-10 column is a high-resolution, high-capacity, mAU Flow: 1 mL/min
Inj. Volume: 5 µL (25 µg)
silica-based HIC column that provides greater hydrolytic stabil- Detection: UV, 214 nm
%B
ity under the highly aqueous conditions used in HIC. Examples Sample: MAb 50 µL (50 mg/mL)
+ 450 µL Eluent B
include the separation of bovine serum proteins, snake venom 0
proteins, enzymes, human skeletal muscle protein (HSMP), –100
0 2.0 4.0 6.0 8.0 10.0 12.2
monoclonal antibodies, pancreatin, and thrombin and peptide
Minutes
applications including tryptic digests of proteins.
21538
Bio Columns
Gradient separation of a monoclonal antibody using the
Separation of a Mixture of Proteins ProPac HIC-10.
Column: 7.5 × 75 mm (Competitors A and B); Sample: Mixture of proteins
7.8 × 75 mm (Dionex ProPac HIC-10) (1 mg/mL each final after
Flow: 1.0 mL/min 1:1 dilution with eluent A)
WVL: 214 nm
Eluents: (A) 2 M (NH4)2 SO4 in 0.1 M NaH2PO4 (pH 7.0)
Inj. Volume: 20 µL
Order of elution: 1. Cytochrome c
Related Literature
(B) 0.1 M NaH2PO4 (pH 7.0) 2. Myoglobin
3. Ribonuclease A
600 4. Lysozyme For detailed specifications and applications, see the following
100
5. Chymotrypsinogen PDF documents under Literature on www.dionex.com.
4
mAU 2 5
1 3
Product Data Sheets
0 Dionex
Competitor A ProPac HIC-10 Column Solutions for Protein Analysis
Competitor B
–300
0 10 20 30 40 50 60 70
Minutes 21286
Separation of a mixture of proteins using the ProPac HIC-10, com-
pared to the same separation on two competitors’ columns. Analytical Columns
ProPac HIC-10 Column (2.1 × 100 mm)......................................................... 063653
ProPac HIC-10 Column (4.6 × 100 mm)......................................................... 063655
ProPac HIC-10 Column (7.8 × 75 mm)........................................................... 063665
www.dionex.com 245
ProPac IMAC Monoclonal Antibody
Analytical column for immobilized metal affinity Column: ProPac IMAC-10 (4 mm x 250 mm)
chromatography 0.010 E1:
E2:
MES + 0.5 M NaCl, pH = 6.0
E1 + imidazole, pH = 6.0
Gradient: t (min) %A %B
1
0 96 4
The ProPac IMAC-10 is an analytical HPLC column packed 15 0 curve 7 100
with 10 µm, nonporous, polymeric beads that are coated with AU 40
Inject Vol: 15 uL
0 100
a hydrophilic layer, then grafted with poly(IDA) chains. The 0.005 Sample: 1.0 mg/mL Mab PDL
poly(IDA) grafts are converted to metal-containing nanoparti- Wavelength: 280 nm
cles when the column is charged with metal. It is these nanopar-
ticles that act as IMAC interaction sites for individual proteins
and provide the ProPac IMAC-10 with its high resolving power. 0.000
–0.001
0 5 10 15 20 25 30 35 40 45 50
• State-of-the-art technology for reusable columns with metal Minutes
20483
tailored specificity
• Resolve target proteins using a single column in a high Separation of monoclonal antibodies using the ProPac IMAC-10.
resolution gradient run
Separation of Standard Proteins Within the Same Class
• Retention control by imidazole concentration or pH gradi-
0.020
ent 2
Column: ProPac IMAC-10 (4 mm x 250 mm)
E1: 20 mM MES + 0.5 M NaCl, pH = 7.5
1 3 E2: E1 + 100 mM imidazole, pH = 7.5
• High purity separations of metal-binding proteins Gradient: t (min) %E1
AU 0 96
• Wide range of metal-specific applications 15 0 curve 7
40 0
Inject Vol: 15 uL
The ProPac IMAC-10 design maximizes the number of steri- 0.000 Sample: 1. 0.25 mg/mL ribonuclease A
–0.002 2. 1.00 mg/mL myoglobin
cally accessible ligands for binding to proteins, ensuring that 0 5 10 15 20 25 30 35 40 3. 1.50 mg/mL carbonic anhydrase
Minutes
the proteins remain tightly bound during separation. It can be Flow Rate: 0.5 mL/min
Wavelength: 280 nm
operated between pH 2–12 and is compatible with commonly Proteins with surface exposed histidine
used protein purification reagents (non-ionic detergents, reduc-

ing agents). It can be used under native or denaturing HPLC
conditions ribonuclease A myoglobin
20416
Bio Columns
The ProPac IMAC-10 is shipped free from any metal and ready Separation of standard proteins with surface-exposed histidines.
to be charged with the metal of your choice. To avoid contact of
the metal solution with your system, charge the IMAC-10 with Separation of Prion-Derived Peptides
Column: ProPac IMAC-10 (4 × 250 mm)
a loading column (an empty column body you fill with your 0.070
3 Eluent: (E1) 20 mM HEPES + 0.5 M NaCl, pH = 7.5
1 2 4
metal solution, then place between the pump and the IMAC-10 7 (E2) E1 + 50 mM imidazole, pH = 7.5
Gradient: t (min) %A
column). 0 95
10 0 curve 7
30 0
AU Inj. Volume: 15 uL
5
Sample: Prion-derived peptides
5i Wavelength: 280 nm
3i 4i 6i
Peak # Sequence
1 PHGGGWGQ
2 PHGXGWGQ
0.000 4 repeats
2 repeats 3 KKRPKP(PHGGGWGQ)2
1 repeat 4 KKRP(PHGXGWGQ)2
–0.007
0 5 10 15 20 25 30 5 KKRPKPWGQ(PHGGGWGQ)4
Minutes 6 WGQ(PHGGGWGQ)4
7 WGA(PHGGGWGA)4
X = sarcosine (N-methyglycine)
1 2 3 4 5 6 7 20420
Separation of prion-related peptides using the IMAC-10.
246 www.dionex.com
ProPac IMAC
Related Literature
Product Data Sheets
ProPac IMAC-10 Column Solutions for Protein and Peptide

Analysis
Analytical Columns
ProPac IMAC-10 Column (1 × 50 mm).......................................................... 063617
ProPac IMAC-10 Column (2x 50 mm)............................................................ 063272
ProPac IMAC-10 Column (2 × 250 mm)........................................................ 063274
ProPac IMAC-10 Column (22 × 250 mm)...................................................... 063282
Accessories
IMAC Loading Column (4 × 50 mm).............................................................. 063667
IMAC Loading Column (9 × 50 mm).............................................................. 063710
IMAC Loading Column (9 × 250 mm)............................................................ 063718
Bio Columns
www.dionex.com 247
ProSwift RP Fast, High Resolution Separation of Proteins
Monolith reversed-phase columns for high-resolution Column: ProSwift RP-2H (4.6 × 50 mm)
Flow Rate: 1, 2, 4, or 8 mL/min
UV Detection:
Sample:
214 nm
Mixture of five proteins
protein separations at high flow rates Eluents: A. DI H2O / CH3CN (95:5; V/V) + 0.1% TFA
B. DI H2O / CH3CN (5:95; V/V) + 0.1% TFA
Inj. Volume:
Order of Elution:
5 µL
1. Ribonuclease A 1.5 mg/mL
Gradient: 1 mL/min: 1-75% B in 12 min 2. Cytochrome C 0.5
2 mL/min: 1-75% B in 6 min 3. BSA 1.5
ProSwift reversed-phase monolith columns uniquely provide the 4 mL/min: 1-75% B in 3 min 4. Carbonic anhydrase 0.9
8 mL/min: 1-75% B in 1.5 min 5. Ovalbumin 1.5
600
advantages of high resolution at exceptionally high flow rates 8 mL/min 4 mL/min 2 mL/min 1 mL/min
for protein separations. They deliver the outstanding resolv- 1
ing power of nonporous analytical media combined with faster 3

4
separations than any bead based columns available. ProSwift mAU
2
polymer monolith columns achieve long column lifetime, ex- 5
hibit reproducibility even after hundreds of runs, and have broad

pH stability, providing high stringent wash capability.
–300
0 1 2 3 4 5 6 7 8 9 10 11
Minutes
• High resolution at high speed 22903
• Highest operational flow rates available Separation of proteins at a variety of flow rates.
• High throughput and improved productivity

Separation of Proteins Using ProSwift RP-2H
• Optimal performance in a broad range of applications Columns: 1. ProSwift RP-2H, 4.6 × 50 mm
2. Competitor A, 4.6 × 100 mm, 15 µm
Inj. Volume:
Temperature:
5 µL
30 °C
Eluents: A. DI H2O / CH3CN (95:5; V/V) + 0.1% TFA Detection: UV, 214 nm
• Excellent stability over a wide pH range of 1 to 14 B. DI H2O / CH3CN (5:95; V/V) + 0.1% TFA Sample: Mixture of five proteins
Gradient: 2 mL/min: 1-75% B in 6 min Peaks: 1. Ribonuclease A 1.5 mg/mL
8 mL/min: 1-75% B in 1.5 min 2. Cytochrome C 0.5
• Outstanding reproducibility and ruggedness Flow Rate: 2 or 8 mL/min 3. BSA 1.5
4. Carbonic anhydrase 0.9
450 5. Ovalbumin 1.5
• High stringent wash compatible, for example, 1 M NaOH
8 mL/min
• High loading capacity ProSwift RP-2H
0
0 1 2
700
ProSwift polymer monolith media are uniquely suited for 2 mL/min
the separation of proteins. Each monolith is a single cylindri- 1 3

ProSwift RP-2H
2 4 5
cal polymer rod containing an uninterrupted, interconnected Competitor A
–100
network of flow-through channels of a specific pore size. These 0 4
Minutes
8
22904
large channels and the monolith’s nonporous surfaces result in
Bio Columns
fast mass transfer for high-resolution and fast protein separa- Comparison of ProSwift RP-2H with porous bead-based column of
tions. The channels also produce low backpressure, allowing the leading competitor A.
use of higher linear velocities with minimal loss of resolution.
248 www.dionex.com
ProSwift RP
Trypsin Digest of Cytochrome C

Column: ProSwift RP-1S (4.6 × 50 mm)
Eluents: A. DI H2O / CH3CN (95:5; V/V) + Analytical Columns
0.1% TFA
B. DI H2O / CH3CN (5:95; V/V) +
0.1% TFA
ProSwift RP-2H Monolith Column (4.6 × 50 mm)......................................... 064296
UV Detection: 214 nm
Sample: Cytochrome C trypsin digest
ProSwift RP-1S Monolith Column (4.6 × 50 mm)......................................... 064297
(2 mg Cytochrome C + 20 µg trypsin
mAU
O/N incubation)
ProSwift RP-3U Monolith Column (4.6 × 50 mm)......................................... 064298
Inj. Volume: 10 µL
-100
0.2 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Minutes
23283
Separation of cytochrome c digest using a ProSwift RP-1S.
Reproducibility of the ProSwift RP-1S

2000
Durability and robustness are ProSwift characteristics.
The ProSwift monoliths exhibit stability and
Injection 506 reproducibility over many runs.
Flow: 4 mL/min
Injection 306 Sample*: 1. Ribonuclease A (1.5 mg/mL)
2. Cytochrome c (0.5 mg/mL)
mAU 3. BSA (1.5 mg/mL)
Injection 206 4. Carbonic Anhydrase (0.9 mg/mL)
5. Ovalbumin (1.5 mg/mL)
Injection: 5 µL
Injection 106
Gradient: 1% B for 1 min, 1 - 75% B in 2.5 min
*Sample is injected every 50 gradient cycles
Injection 6
–20
0 1 2 3 4
Minutes
23136
Bio Columns
Reproducibility and ruggedness of the ProSwift RP-1S ensure long
column lifetime.
Related Literature
Product Data Sheets
ProSwift and PepSwift Monolith Columns for Biomolecule

Analysis
www.dionex.com 249
ProSwift IEX ProSwift SAX versus the Competition
Monolith ion-exchange columns for superior Columns: ProSwift SAX-1S, 4.6 × 50 mm
high-resolution analytical and preparative protein Leading competitor Q, 5 × 50 mm
Eluents: A. 10 mM Tris, pH 7.6
separations B. 10 mM Tris + 1 M NaCl, pH 7.6
ProSwift IEX monoliths are the column of choice when high 1
Inj. Volume:
Detection:
10 µL
UV, 214 nm
resolution and capacity are required for protein separations. mAU Sample: Protein mixture, 1 mg/mL each:
They deliver higher resolution than porous bead-based columns 3
1. Myoglobin
2. Conalbumin
while providing high capacity. ProSwift IEX monoliths provide 4 3. Ovalbumin
2 Leading Competitor Q 4. Trypsin inhibitor
the outstanding resolving power of nonporous analytical media
combined with fast separation performance. ProSwift polymer 0 ProSwift SAX
monolith columns achieve long column lifetime and exhibit

0 3 6 9
Minutes
reproducibility even after hundreds of runs. 24911
• High resolution Comparison of the separation of a protein mixture on the ProSwift

SAX-1S and a leading competitor’s column.
• High loading capacity
• Fast separations
Separation of MAbs Using the ProSwift WCX-1S Column
• Wide range of operational flow rates
• Excellent stability over a wide pH range 600
Column: ProSwift WCX-1S (4.6 × 50 mm)

• Outstanding reproducibility and ruggedness Flow rate: 1 mL/min
K Eluents: A. 10mM Sodium phosphate
(pH 7.6)
• Optimal performance in a broad variety of applications mAU KK B. 1 M Sodium chloride in eluent A
Sample: MAb 5 mg/mL
• High throughput and improved productivity Acidic Inj. Volume: 10 µL
variants Gradient: 5–10% B in 10 min, 100% B for 4 min
Basic variants
ProSwift polymer monolith media are uniquely suited for the –100
separation of proteins. Each monolith is a single cylindrical, 0 Minutes 14.1
sponge-like polymer rod containing an uninterrupted, intercon-

nected network of flow-through channels of a specific pore size. 23839
Bio Columns
These large channels and the monolith’s nonporous surfaces

result in fast mass-transfer, for high-resolution and fast protein Separation of monoclonal antibody variants.
separations. The unique globular morphology of the polymer
medium provides its high capacity.
250 www.dionex.com
ProSwift IEX
Separation of E. coli Proteins Using a Ordering Information

ProSwift WAX-1S Column
Column: ProSwift WAX-1S (1 × 50 mm)
Eluents: A. 10 mM Tris, pH 7.6 Analytical Columns
B. 1 M NaCl in 10 mM Tris, pH 7.6
Gradient: 5 to 55% of B in 13 min,
Hold for 2 min
ProSwift SAX-1S Monolith Column (4.6 × 50 mm)...................................... 064293
Temperature: 30 °C
ProSwift SCX-1S Monolith Column (4.6 × 50 mm)....................................... 066765
ProSwift SAX-1S Monolith Column (1.0 × 50 mm)...................................... 068459
mAU Sample: 1.25 mg/mL E. coli protein
ProSwift WAX-1S Monolith Column (4.6 × 50 mm)..................................... 064294
ProSwift WCX-1S Monolith Column (4.6 × 50 mm)..................................... 064295
ProSwift WAX-1S Monolith Column (1 × 50 mm)........................................ 066642
ProSwift WCX-1S Monolith Column (1 × 50 mm)........................................ 066643
0 5 10 15
Minutes
24270
Protein separation on the ProSwift WAX-1S column.
Separation of Hemoglobin
Using a ProSwift SCX-1S Column
Column: ProSwift SCX-1S (4.6 x 50 mm)
Flow Rate: 1 mL/min (bottom), 2 mL/min (top)
Eluents: A. 50mM NaPO4, pH 6, 2 mM NaCN
B. 0.5M NaCl in eluent A
Gradient: 1–13% B in 15 min (1 mL/min)
in 7.5 min (2 mL/min)
mAU Detection: UV at 280 nm
Temperature: 30 °C
Inj. vol: 2 µL
Sample: Hemoglobin A2 column control
0 Minutes 20 25173
Bio Columns
Separation of hemoglobin at different flow rates using a ProSwift SCX
column.
Related Literature
Product Data Sheets
ProSwift Ion-Exchange Monolith Columns for Biomolecule

Analysis Data Sheet
www.dionex.com 251
Acclaim 300 C18 Cytochrome c Tryptic Digest
High-resolution separation of proteins and peptides
0.50
The Acclaim 300 features 3 µm silica particles for rapid analysis
0.40 Column: Acclaim 300 C18
of complex protein digests. The Acclaim 300’s stable bonding Mobile Phase: (A) 95/5 water/acetonitrile,
0.1% trifluoroacetic acid;
make these columns compatible with a wide variety of applica- 0.30
(B) 95/5 acetonitrile/water,
AU
tions, including LC/MS methods. 0.20
Gradient:
0.1% trifluoroacetic acid
100% A/0% B to 65% A/
35% B at 45 min
0.10 Flow Rate: 1.0 mL/min
• Designed for high-resolution peptide mapping and protein Temperature: 30 °C
separations –0.05
Inj. Volume: 40 µL
5 10 15 20 25 30 35 40 45
• High-efficiency 3 µm spherical silica substrate Minutes
• High-performance bonding chemistry on 300 Å pore silica

20395
• Application tested for suitablility in peptide mapping

Cytochrome c tryptic digest.
• Reproducible for dependable results
• LC/MS compatible Comparison of Acid Additives for Peptide Analysis on Acclaim 300
2
Column: Acclaim 300 C18, 3 µm,
Manufacture of these columns starts with ultrapure silica that 4.6 × 150 mm
Pump: Summit P680A
contains minimal concentrations of metal contaminants, mini- A Mobile Phases: A: 70% acetonitrile v/v
B: Water
mizing the tailing effects of residual, exposed silanol groups. C: Acid stock solution
Exhaustive bonding and end-capping techniques result in stable AU
B
Gradient Times: –8 0 16
%A 7 7 75
bonding and columns that exhibit predictable reversed-phase %B 68 68 0
%C 25 25 25
separations with minimal secondary interactions. Flow: 1.0 mL/min
C
Temperature: TCC100 thermostat at 30 °C
0 Injection: ASI-100 autosampler; 5 µL
The use of a 3 µm silica particle reduces limits for diffusion of 2 4 6 8 10 12 Detector: UVD 340U; UV at 214 nm
Minutes Acid Additive: A. Phosphoric Acid, 0.15%
the analytes into the stationary phase, resulting in fast, high- Peaks: 1. Gly-Tyr B. Trifluoroacetic Acid, 0.1%
C. Formic Acid, 0.1%
resolution separations. Compared to 5 µm column packings, the 2. Val-Tyr-Val
Sample: Sigma H2016 Peptide Mixt
3. Met-Enkephalin
smaller particles support increased flow rates and shallower gra- 4. Angiotensin-II
5. Leu-Enkephalin
dients on shorter columns, for faster separations. Dionex tests 23064
Bio Columns
each bonded silica lot for peptide selectivity to ensure reproduc- Comparison of acid additives for peptide analysis.
ible performance.
252 www.dionex.com
Acclaim 300 C18
Capillary LC for Peptide Mapping Using Acclaim® 300 C18

22 The standard particle size is nominally 3 µm. Analytical col-
umns are available in 2.1, 3.0, and 4.6 mm dimeters; standard
Column: Acclaim 300 C18, 3 mm
lengths are 50 and 150 mm. Capillary formats are available in
Dimensions: 75 µm i.d. × 15 cm 75 and 300 µm, 1.0 mm, and custom diameters, and in 50, 150,
Mobile Phase: (A) 95/5 (v/v) H2O/acetonitrile,
0.05% TFA or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm
mAU (B) 20/80 (v/v) H2O/acetonitrile,
0.04% TFA sizes are recommended to protect analytical columns.
Sample: Tryptic digest of BSA,1 pmol/mL
Analytical Columns
–2 Acclaim 300, C18, 3 µm Analytical (2.1 × 50 mm)....................................... 060263

15 20 25 30 35 40 45 50 55 60 65 70
Minutes
20408
Acclaim 300, C18, 3 µm Analytical (3 × 150 mm)........................................ 063684
Capillary LC for peptide mapping. Acclaim 300, C18, 3 µm Analytical (4.6 × 50 mm)....................................... 060265
Protein Mixture by Reversed-Phase HPLC on Acclaim® 300 C18

Column: Acclaim 300 C18, 3 mm
Guard Columns
1,000
Mobile Phase: (A) 5 mM MSA in 95/5 H2O/acetonitrile Acclaim 300 Guard Cartridges (2 × 10 mm), 2 each..................................... 060395
(B) 5 mM MSA in 5/95 H2O/acetonitrile
4 Inj. Volume: 25 µL Acclaim 300 Guard Cartridges (4.3 × 10 mm), 2 each.................................. 060393
Gradient: Time %A %B Flow
mAU
0
3
100
100
0
0
1.0 Hardware
18 0 100
1
7 23 0 100 SST Guard Cartridge Holder.......................................................................... 059456
Peaks: 1. RNAse A
6
2. Impurity in carbonic anyhdrase Guard to Analytical Column Coupler............................................................ 059457
3 5 3. Impurity in lysozyme
2 4. Lysozyme Guard Kit (Holder and Coupler)..................................................................... 059526
5. Impurity in carbonic anhydrase
–50
6. Myoglobin
6 8 10 12 14 16 7. Carbonic anhydrase
Minutes Micro and Nano Columns
20407
75 µm i.d. × 5 cm, C18 Acclaim, 3 µm, 300 Å............................................... 162223
Bio Columns
Protein mixture separated using reversed-phase HPLC. 75 µm i.d. × 15 cm, C18 Acclaim, 3 µm, 300 Å............................................. 162224
300 µm i.d. × 5 cm, C18 Acclaim, 3 µm, 300 Å............................................. 162221
300 µm i.d. × 15 cm, C18 Acclaim, 3 µm, 300 Å........................................... 162222
Related Literature
1.0 mm i.d. × 5 cm, C18 Acclaim, 3 µm, 300 Å............................................. 162219
For detailed specifications and applications, see the following 1.0 mm i.d. × 15 cm, C18 Acclaim, 3 µm, 300 Å........................................... 162220
PDF documents under Literature on www.dionex.com. These columns are designed for optimal performance using
ments.
Product Data Sheets
Acclaim 300 HPLC Columns Data Sheet
Application Updates
AU 156: Evaluation of Acclaim HPLC Columns Using the Na-

tional Institute of Standards Standard Reference Material 870
Technical Notes
TN 705: Automated Enrichment and Determination of Phospho-

peptides Using Immobilized Metal Affinity and Reversed-Phase
Chromatography with Column Switching
www.dionex.com 253
Strong Cation Exchange
Strong cation-exchange separations for peptides
Multidimensional LC of peptides is often performed by combin-
ing strong cation-exchange (SCX) and reversed-phase (RP) sep-
arations in on-line and offline setups. For on-line 2-D LC setups
(e.g., 2-D salt plugs), the flow through particles of the Poros 10S
SCX column generate very little backpressure without compro-
mising efficiency. For offline 2-D LC setups (e.g., automated
offline), the Polysulfoethyl ASP provides an efficient separation
of even the most complex peptide samples.
Analytical Columns
300 µm i.d × 10 cm, Polysulfoethyl ASP, 5 µm, 300 Å.................................. 164264
300 µm i.d. × 15 cm, Polysulfoethyl ASP, 5 µm, 300 Å................................. 164263
1.0 mm i.d. × 15 cm, Polysulfoethyl ASP, 5 µm, 300 Å................................. 164262
300 µm i.d × 10 cm, Poros 10S...................................................................... 163027
300 µm i.d. × 10 cm, Poros 10S w/ connection tubing for 2-D salt plugs... 162152
300 µm i.d. × 15 cm, Poros 10S..................................................................... 162122
1.0 mm i.d × 15 cm, Poros 10S...................................................................... 163031
Bio Columns
254 www.dionex.com
Acclaim PepMap
Acclaim PepMap Acclaim PepMap100 C18
The members of the Acclaim PepMap family are designed to

Tryptic Digest of a Protein Mixture on a Pepmap™
separate peptides and proteins efficiently. These silica-based C18 Nanocolumn
stationary phases have virtually zero silanophilic activity, result-
ing in minimal peak tailing. The Acclaim PepMap columns can 1.25 Nanocolumn: 75 µm i.d. x 15 cm,
PepMap100 C18, 3 µm, 100 Å
be operated TFA-free, making them ideal for LC-MS based BPC Temperature: Ambient
Flow Rate: 300 nL/min
biomolecular analysis. Solvents: (A) H2O/acetonitrile (98:2 v/v),
0.1 % formic acid
Intens. (B) H2O/acetonitrile (20:80 v/v),
• Zero silanophilic interactions x 107 0.08 formic acid
MS: esquire3000 (Bruker)
• Peptide separations: C18 and C8 100 Å with 3 µm or 5 µm Sample: Tryptic digest of a protein mixture:
particles cytochrome c, lysozyme,
alcohol dehydrogenase,
0.00 bovine serum albumin,
• Protein separations: C18 and C4 300 Å with 5 µm particles 0 10 20 30 40 50 60 70 80 90 100
Minutes
apotransferrin, β-galactosidase,
500 fmol each
• TFA Free LC/MS, for enhanced MS sensitivity

21054
• Outstanding separation efficiency

• Superior resolution and recovery Acclaim PepMap100 C18 sets the benchmark in peptide
separation. PepMap100 C18 is available in 3 or 5 µm particle
Acclaim PepMap Trap and Nano-Trap columns are available for sizes, with 100 Å pore sizes, and in nano, capillary, and micro
preconcentration of diluted samples, large-volume injections, formats.
or various column-switching techniques. Allowing substantially
shorter loading times results in reduced overall analysis time.
Desalting or SDS removal prior to MS analysis, phosphopeptide Ordering Information
enrichment, and MDLC (2-D, 3-D) are other applications.
Nano LC Columns
75 µm i.d. × 5 cm, Acclaim PepMap100 C18, 3 µm, 100 Å.......................... 160316
Related Literature 75 µm i.d. × 15 cm, Acclaim PepMap100 C18, 3 µm, 100 Å........................ 160321
For detailed specifications and applications, see the following 75 µm i.d. × 25 cm, Acclaim PepMap100 C18, 3 µm, 100 Å........................ 164261
PDF documents under Literature on www.dionex.com. 75 µm i.d. × 5 cm, Acclaim PepMap100 C18, 5 µm, 100 Å.......................... 160318
Bio Columns
75 µm i.d. × 15 cm, Acclaim PepMap100 C18, 5 µm, 100Å......................... 160323
Application Notes 75 µm i.d. × 25 cm, Acclaim PepMap100 C18, 5 µm, 100 Å........................ 160326
AN 521: Automated 2-D LC Coupled to ESI-MS/MS for the Capillary LC Columns

Analysis of Complex Peptide Samples
300 µm i.d. × 5 cm, Acclaim PepMap100 C18, 3 µm, 100 Å........................ 160290
AN 524: Parallel Nano and Capillary LC for High-Throughput 300 µm i.d. × 15 cm, Acclaim PepMap100 C18, 3 µm, 100 Å...................... 160295
MS Proteomics
AN 527: Comprehensive 2-D Nano LC/MS for Human Tissue 300 µm i.d. × 15 cm, Acclaim PepMap100 C18, 5 µm, 100 Å...................... 160297
Proteomics 300 µm i.d. × 25 cm, Acclaim PepMap100 C18, 5 µm, 100 Å...................... 160300
AN 531: Phosphopeptide Enrichment using a TiO2 Nano

Precolumn Micro LC Columns
1.0 mm i.d. × 5 cm, Acclaim PepMap100 C18, 3 µm, 100 Å........................ 160277
Technnical Notes 1.0 mm i.d. × 15 cm, Acclaim PepMap100 C18, 3 µm, 100 Å...................... 160282
TN 60: UltiMate 3000: Direct Sample Injection onto a
1.0 mm i.d. × 15 cm, Acclaim PepMap100 C18, 5 µm, 100 Å...................... 160284
75 μm i.d. PepMap100 (C18) Column
1.0 mm i.d. × 25 cm, Acclaim PepMap100 C18, 5 µm, 100 Å...................... 160287
TN 61: UltiMate 3000 — Preconcentration on a
75 μm i.d. × 15 cm PepMap100 (C18) Nanocolumn
www.dionex.com 255
Acclaim PepMap300 C18 Ordering Information
PepMap 300 C18 for Superior Protein Separations Nano LC Columns

75 µm i.d. × 5 cm, Acclaim PepMap100 C8, 3 µm, 100Å............................. 161184
Column: 300 µm i.d. x 15 cm,
mAU PepMap300, C18, 5 µm 75 µm i.d. × 15 cm, Acclaim PepMap100 C8, 3 µm, 100Å........................... 161185
300 Å,
150 Temperature: 40 ºC 75 µm i.d. × 5 cm, Acclaim PepMap100 C8, 5 µm, 100Å............................. 161555
Flow Rate: 4 µL/min
100 Mobile Phase: A: H2O/acetonitrile 75 µm i.d. × 15 cm, Acclaim PepMap100 C8, 5 µm, 100Å........................... 161553
(90/10 v/v), 0.1% TFA
B: H2O/acetonitrile 75 µm i.d. × 25 cm, Acclaim PepMap100 C8, 5 µm, 100Å........................... 161186
50
(10/90 v/v), 0.08% TFA
0 Detection:
Sample:
UV, 214 nm
Ribonuclease, insulin,
Capillary LC Columns
-50 cytochrome c, myoglobin,
lysosyme, 40 ng/protein 300 µm i.d. × 5 cm, Acclaim PepMap100 C8, 3 µm, 100 Å.......................... 161181
0 10 20 30
Minutes
24779
Acclaim PepMap300 C18 is used for the separation of larger
peptides and proteins. It is available in 5 µm particle sizes, with Micro LC Columns
300 Å pore sizes, and in nano, capillary, and micro formats.
1.0 mm i.d. × 5 cm, Acclaim PepMap100 C8, 3 µm, 100 Å.......................... 160240
1.0 mm i.d. × 5 cm, Acclaim PepMap100 C8, 5 µm, 100 Å.......................... 161539
Nano LC Columns 1.0 mm i.d. × 25 cm, Acclaim PepMap100 C8, 5 µm, 100 Å........................ 161180
75 µm × 5 cm, Acclaim PepMap300 C18, 5 µm, 300 Å wide pore.............. 163577
75 µm × 15 cm, Acclaim PepMap300 C18, 5 µm, 300 Å wide pore............ 163574
Acclaim PepMap300 C4
Capillary LC Columns Acclaim PepMap300 C4 is used for the separation of hydro-
phobic peptides and proteins, providing higher recoveries. It
Bio Columns
300 µm × 5 cm, Acclaim PepMap300 C18, 5 µm, 300 Å wide pore............ 163580
is available in 5 µm particle size, with 100 Å pore size, and in
300 µm × 15 cm, Acclaim PepMap300 C18, 5 µm, 300 Å wide pore.......... 163581
nano, capillary, and micro formats.
Micro LC Columns
1.0 mm × 5 cm, Acclaim PepMap300 C18, 5 µm, 300 Å wide pore............ 163584
1.0 mm × 15 cm, Acclaim PepMap300 C18, 5 µm, 300 Å wide pore.......... 163585
Capilary LC Columns
Acclaim PepMap100 C8
Acclaim PepMap100 C8 is a excellent alternative for the Ac- 300 µm × 5 cm, Acclaim PepMap300 C4, 5 µm, 300 Å .............................. 163582
claim PepMap100 C18, when separating very hydrophobic 300 µm × 15 cm, Acclaim PepMap300 C4, 5 µm, 300 Å.............................. 163583
peptides (e.g., non-tryptic peptides). It is available with 3 or
5 µm particles, with 100 Å pore sizes, and in capillary and Micro LC Columns
micro formats.
1.0 mm × 5 cm, Acclaim PepMap300 C4, 5 µm, 300 Å................................ 163586
1.0 mm × 15 cm, Acclaim PepMap300 C4, 5 µm, 300 Å.............................. 163587
256 www.dionex.com
Acclaim PepMap
Acclaim PepMap µ-Precolumns Acclaim PepMap µ-Guard Columns
Acclaim PepMap µ-Precolumns are very short microcolumns– Acclaim PepMap µ-Guard columns are short microcolumns that
available in 5 and 15 mm lengths–consisting of a cartridge can be coupled directly to the front of the capillary or micro LC
holder and a set of disposable cartridges. µ-Precolumns are used column to avoid column contamination.
for sample pre-concentration, sample cleanup, or in various
column-switching techniques.
µ-Guard Columns
300 µm i.d. × 1 mm, Acclaim PepMap100 C18, 5 µm, 100 Å...................... 160428
µ-Precolumns
µ-Precolumn holder, 5 mm, with universal fitting........................................ 160431 300 µm i.d. × 1 mm, Acclaim PepMap100 C8, 5 µm, 100 Å........................ 161188
µ-Precolumn holder, 15 mm, with universal fitting...................................... 160432 300 µm i.d. × 1 mm, Acclaim PepMap300 C4, 5 µm, 300 Å........................ 163937
300 µm i.d. × 5 mm, Acclaim PepMap100 C18, 5 µm, 100 Å 800 µm i.d. × 2 mm, Acclaim PepMap100 C18, 5 µm, 100 Å...................... 160424
(set of 5 cartridges)....................................................................................... 160454
300 µm × 5 mm, Acclaim PepMap300 C18, 5 µm, 300 Å
(set of 5 cartridges)....................................................................................... 163589 800 µm i.d. × 2 mm, Acclaim PepMap100 C8, 5 µm, 100 Å........................ 161187
300 µm i.d. × 5 mm, Acclaim PepMap100 C8, 5 µm, 100 Å 800 µm i.d. × 2 mm, Acclaim PepMap300 C4, 5 µm, 300 Å........................ 163941
300 µm × 5 mm, Acclaim PepMap300 C4, 5 µm, 300 Å
Acclaim PepMap100 C18 Nano-Trap
500 µm i.d. × 5 mm, Acclaim PepMap100 C18, 5 µm, 100 Å Columns
The Acclaim PepMap100 Nano-Trap columns are available in
500 µm i.d. × 15 mm, Acclaim PepMap100 C18, 5 µm, 100 Å
100 µm and 200 µm i.d. fused silica, and are packed with 5 µm
particle size and 300 Å pore size.
500 µm i.d. × 5 mm, Acclaim PepMap100 C8, 5 µm, 100 Å Ordering Information
Bio Columns
Nano-Trap Columns
500 µm i.d. × 5 mm, Acclaim PepMap300 C4, 5 µm, 300 Å Nano Trap Column, 100 µm i.d. × 1 cm, packed with Acclaim
(set of 5 cartridges)....................................................................................... 163943 PepMap100 C18, 5 µm, 100 Å (set of 2)....................................................... 164197
500 µm i.d. × 15 mm, Acclaim PepMap300 C4, 5 µm, 300 Å Nano Trap Column, 100 µm i.d. × 2 cm, packed with Acclaim
1.0 mm i.d. × 5 mm, Acclaim PepMap100 C18, 5 µm, 100 Å Nano Trap Column, 200 µm i.d. × 1 cm, packed with Acclaim
1.0 mm i.d. × 15 mm, Acclaim PepMap100 C18, 5 µm, 100 Å Nano Trap Column, 200 µm i.d. × 2 cm, packed with Acclaim
1.0 mm × 5 mm, Acclaim PepMap300 C18, 5 µm, 300 Å
1.0 mm i.d. × 5 mm, Acclaim PepMap100 C8, 5 µm, 100 Å
1.0 mm i.d. × 15 mm, Acclaim PepMap100 C8, 5 µm, 100 Å
www.dionex.com 257
Titanium-Dioxide Nano-Trap Columns PepSwift
Monolithic columns for fast, high-resolution protein
Isolation of Two Synthethic Phosphopeptides from a
and peptide analysis
BSA Tryptic Digest on a TiO2 Column
x108 Breakthrough A
Column: 200 µm i.d. x 1 cm, packed with
TiO2, 5 µm
PepSwift monolithic columns are specially designed for fast
4 100 µm I.d. x 1 cm, packed with separation and identification of proteins and peptides using nano
Acclaim® PepMap
3
C18 PM100, 5 µm and capillary LC coupled to MS. Pepswift columns are based on
2 Analytical Column: Acclaim PepMap C18 PM100, 3 µm
1
Dimensions: 75 µm x 15 cm a polystyrene divinylbenzene copolymer bed. The bed structure
Loading Solvent: 0.05% HFBA in DI H2O
0 Wash Solvent: 0.01% HFBA in DI H2O of the column offers a high-quality alternative to traditional
x108 Bound fraction B Mobile Phases: (A) 0.05% TFA in DI H2O
4 (B) 0.04% TFA in acetronitrile/ DI H2O microparticulate sorbents, providing important advantages for
(80:20 v/v)
3
ENIMRpSENSESQLTSK
1931.8 Da SVENLPEAGlpTHEQR
1758.8 Da Gradient: 3–40% acetronitrile in 30 min
high-sensitivity proteomics applications.
2 TiO2 Trap Eluent: 250 mM NH4HCO3 in DI H2O, pH 9.0
Flow Rate: 300 nL/min
1 Loading Flow: 8 µL/min • Polymeric monolithic stationary phases
0
Inj. Volume: 5 µL
Detection: MS
5 10 15 20 25
Minutes
30 35 40 45
• High-speed protein/peptide separations (<10 min)
23731
• High separation efficiency (>200.000 plates/m)
• High column-to-column reproducibility
The Titanium-Dioxide Nano-Trap columns support the enrich-
ment of phosphopeptides. They are available in 100 µm and • High sensitivity in LC/MS
200 µm i.d. format, and are packed with 5 µm particle size. In
addition, a combination of Titanium-Dioxide and Acclaim Pep- • Superior lifetime
Map100 C18 is also available. • Available in 100 µm, 200 µm and 500 µm i.d
Note: See Application Note 531: Phosphopeptide Enrichment PepSwift Precolumns can be used for preconcentration and
Using a TiO2 Nano Precolumn for more information. desalting of samples consisting of peptides and proteins without
negative impact on the chromatographic performance or recov-
ery of the compounds. Various ion-pairing agents can be used in
Ordering Information the loading solvent and/or mobile phases to change the selectiv-
ity of the separation or improve the trapping efficiency.
Nano-Trap Columns
Bio Columns
Nano Trap Column, 100 µm i.d. × 1 cm,TiO2, 5 µm (set of 2)....................... 164205 Separation of a Peptide Test Mixture
Nano Trap Column, 100 µm i.d. × 2 cm,TiO2, 5 µm (set of 2)....................... 164214 Using the PepSwift Capillary Column
Nano Trap Column, 200 µm i.d. × 1 cm,TiO2, 5 µm (set of 2)....................... 164215 4 Column: PepSwift Capillary RP, 200 µm × 50 mm
Eluents: A. DI H2O + 0.05% TFA
Nano Trap Column, 200 µm i.d. × 2 cm,TiO2, 5 µm (set of 2)....................... 164206 B. Acetonitrile / DI H2O (1:1) + 0.04% TFA
7 Gradient: 0–50% B in 7.5 min
Nano Trap Column, 100 µm i.d. × 2 cm, packed with 1cm TiO2, 5 µm Flow Rate: 2.5 µL/min
2 4 Inj. Volume: 100 nL
and 1 cm Acclaim PepMap100 C18, 5 µm, 100 Å (set of 2)........................ 164216 3 5 Temperature: 60 °C
6 Detection: UV, 214 nm
Nano Trap Column, 200 µm i.d. × 2 cm, packed with 1 cm TiO2, 5 µm mAU
Sample: Mixture of Peptides
and 1 cm Acclaim PepMap100 C18, 5 µm, 100 Å (set of 2)........................ 164217 1
89
Peaks: 1. Bradykinin fragments 1–5
2. Vasopressin [Arg8]
3. Methionine enkephalin
4. Leucin enkephalin
5. Oxytocin
–2 6. Bradykinin
0 4 8 7. LHRH
Minutes 8. Bombesin
9. Substance P
19846
Separation of a peptide test mixture.
258 www.dionex.com
PepSwift
Related Literature
Peak Width at Half Height (PWHH) for
Peptides Separated on a Monolithic Columns For detailed specifications and applications, see the following
Peptide Number Retention Time PWHH PDF documents under Literature on www.dionex.com.
Minutes Seconds
1. Bradykinin fragment 1–5 3.3 3.5 Application Notes
2. Vasopressin [Arg 8] 3.8 1.6
3. Methionine enkephalin 4.0 1.9 AN 522: Monolithic Capillary Columns in LC-MS Proteomics
4. Leucine enkephalin 4.4 2.3
5. Oxytocin 4.6 1.6
AN 523: Fast Protein and Peptide Separations Using Monolithic
6. Bradykinin 4.9 2.5 Nanocolumns and Capillary Columns
7. LHRH 5.1 1.9
AN 525: LC-MALDI MS Using Monolithic Capillary Columns
8. Bombesin 5.1 1.9
9. Substance P 6.4 2.6 AN 526: PS-DVB Monolithic Columns Applied in an Off-Line
2-D LC/ESI-MS Bottom-Up Study for the Identification of
Peak Width at Half Height (PWHH) for peptide separation. Platelet Proteins
AN 528: Parallel LC with Capillary PS-DVB Monolithic Col-

Separation of a Peptide Test Mixture
umns for High-Throughput Proteomics
Using the PepSwift Capillary Column
AN 529: Capillary PS-DVB Monolithic Column of 500-μm i.d.
Column: PepSwift 200 µm i.d. x 5 cm, for Peptide and Protein Separations in Top-Down Proteomics
10 PS-DVB
Eluents: A1. DI H2O + 0.1% FA Studies
8 B1. Acetonitrile / DI H2O (1:1) + 0.08% FA
A2. DI H2O + 0.05% TFA
FA B2. Acetonitrile / DI H2O (1:1) + 0.04% TFA
AN 530: Proteome Analysis Involving Off-Line 2-D LC of In-
6
mAU Flow Rate:
2.5 µL/min tact Proteins, Proteolytic Digestion, and Capillary RP-LC-MS/
Flow Cell:
Nanoflow cell,
4
3 nL volume
MS Analysis Using Monolithic PS-DVB Columns
TFA Detection: UV, 214 nm
0 Ordering Information
–1
0 2 4 6 8 10 12 14
Minutes
19847 Analytical Columns
Bio Columns
Comparison of the influence of FA and TFA on separation of a peptide Monolithic Nano Column, 100 µm i.d. x 5 cm (PS-DVB).............................. 162348
mixture. Monolithic Capillary Column, 200 µm i.d. x 5 cm (PS-DVB)......................... 161409
Monolithic Capillary Column, 500 µm i.d. x 5 cm (PS-DVB)......................... 164087
The Fast Separation of Triptic Peptides from 13 Proteins
Micro and Nano Precolumns
Intens.
x10 7 Monolithic Trap Column, 200 µm i.d. x 5 mm (PS-DVB), set of 2................ 163972
2.5
2.0
PWHH just 3s
1.5
1.0
0.5
0.0
2 4 6 8 10 12 14
Minutes
24780
Fast separation of tryptic peptides from 13 proteins. Peak capacities of

up to 150 peaks in less than 15 min are routinely observed.
www.dionex.com 259
Bio Columns
260 www.dionex.com
Nucleic Acid Columns
HPLC analysis of nucleic acids
The DNAPac series of polymer-based, anion-exchange col-
umns set the standard for oligonucleotide purity analysis, fast
screening, and purification. Two column types are available:
DNAPac PA100 and PA200. Both are strong anion-exchange
columns developed for high-resolution analysis and purifica-
tion of synthetic and modified oligonucleotides. The DNAPac
PA200 offers improved efficiency, and enhanced stability under
alkaline conditions. DNAPac PA100: Anion-exchange column for n, n–1 resolution
of single-stranded oligonucleotides.
• High resolution separation of oligonucleotides and nucleic
acids DNAPac PA200: Polymer-based, anion-exchange column for
oligonucleotide purity check, screening, and purification.
• Capable of n, n-1 resolution for oligonucleotides
• Compatible with solvent, high pH, and high temperatures
• Provides easy scale-up
These columns support screening of synthetic oligonucleotides

for production yield and failure sequences on a routine basis;
unit-base resolution of synthetic oligonucleotides has been dem-
onstrated for up to 60 bases and beyond. These columns provide
unsurpassed high-resolution of full length from n–1, n+1, and
other failure sequences not possible with other columns.
Bio Columns
www.dionex.com 261
DNAPac PA100 Separates RNA / Double-Stranded DNA
For n, n-1 resolution of single-stranded The DNAPac PA100 is ideally suited for the purification and
oligonucleotides analysis of synthetic RNA. Failure sequences are easily sepa-
rated from the full-length product. Double-stranded DNA, such
The DNAPac PA100 is a high-resolution anion-exchange colas plasmids, or restriction fragments are also separated.
umn that provides single-base resolution. It is stable under dena-
turing conditions, rugged, reliable, and can be readily scaled up.
Analytical separations on the 4 mm diameter column can be
The DNAPac PA100 is a 13 µm pellicular, nonporous polymeric
scaled directly to larger diameter columns so preparative meth-
resin with bound quaternary amine-functionalized MicroBeads.
ods can be conveniently developed using small samples. The
The rapid mass-transport characteristics of this resin result in
loading capacity of the DNAPac PA100 (4 × 250 mm) column
very high-resolution oligonucleotide separations.
is from 1 to 100 µg. Scaling the flow rate and sample size up
• Capable of n, n-1 resolution for oligonucleotides for the 9 × 250 mm and 22 × 250 mm column yields essentially
identical chromatography.
• Resolves oligonucleotides with secondary structures
• Compatible with solvent, high pH, and high temperatures
Related Literature
• Analyzes phosphorothioate-based clinical samples
• Provides easy scale-up
The MicroBead resin used in DNAPac columns provides
high-resolution separations to ensure maximum peak purity, Product Data Sheets
and makes linear scale-up of your separation easy. For more
complex separations, the polymeric properties of the DNAPac DNAPac PA100 Data Sheet
PA100 allows great flexibility to select ideal conditions, includ-
Application Notes
ing high pH, high temperature, or chaotropic agents.
AN 100: High-Resolution Analysis and Purification of Oligo-
Synthetic oligonucleotides can be screened for production yield nucleotides with the DNAPac PA100 Column
and failure sequences using the DNAPac PA100. Unit-base res-
olution of synthetic oligonucleotides to 60 bases and beyond has
been demonstrated. The DNAPac PA100 can even be operated Ordering Information
Bio Columns
under denaturing conditions. Either high temperature (up to 90

°C) or high-pH eluents (up to pH 12.5) can be used to eliminate Analytical Columns
hydrogen bonding, allowing resolution of problem sequences
such as self-complementary sequences or poly-G stretches. DNAPac PA100 Analytical Column (4 × 250 mm)......................................... 043010
DNAPac PA100 Semi-Preparative Column (9 × 250 mm)............................ 043011
DNAPac PA100 Semi-Preparative Column (22 × 250 mm)..........................SP2091
Separation of 1.5 µg of pd(A)40–60 FAST Cartridge, DNAPac PA100 (2 × 10 mm), Pkg/150...............................SP4008
Using the DNAPac® PA100 Column
FAST Cartridge, DNAPac PA100 (2 × 10 mm), Pkg/600...............................SP3229
0.004
Guard Columns
Column: DNAPac PA100 DNAPac PA100 Guard Column (2 × 50 mm).................................................SP4016
AU Eluent: 410–510 mM NaCl in
25 mM Tris-Cl, pH 8.0 DNAPac PA100 Guard Column (4 × 50 mm)................................................. 043018
Sample: pd(A)40–60
–0.001
5 10 15 20 25
Minutes
10954
Separation of oligonucleotides using the DNAPac PA100.
262 www.dionex.com
DNAPac PA200
DNAPac PA200 Separation of Oligonucleotides by Length
15
Ultrahigh-resolution separations of oligonucleotides Column: DNAPac® PA200 Oligonucleotide length: 30.1%
Eluent: NaClO4, pH 6.5
with 20% CH3CN 21 22 23 24 25
The DNAPac PA200 is a strong anion-exchange column Inj. Volume: 8 µL
Dx98
Dx96
Dx94
Dx91
Dx90
Dx88
Dx86
Dx87
D84
D80
developed to provide unsurpassed high-resolution analysis and Detection: UV, 260 nm
purification of synthetic oligonucleotides. DNAPac PA200 can

resolve full length from n–1, n+1, and other failure sequences
mA260
not possible with other columns. Retention times and selectivity
can be controlled by the choice of salt, pH, and solvent. There-
fore, the separation can be tailored to the requirements of many
0.33 M NaClO4:
different oligonucleotide analysis challenges. 22.8%
0
• Achieve n, n-1 resolution for oligonucleotides 3 4 5 6 7
Minutes 21024
• Resolve oligonucleotides with secondary structures
Separation of oligonucleotides by length.
• Assay phosphorothioate purity
• Selectivity control with eluent pH, salt, and solvent Analytical separations on the 4 mm diameter column can
be scaled directly to larger diameter columns so preparative
• Resolve RNA with aberrant (2’–5’) links from normal SS- methods can be conveniently developed using small samples.
RNA Scaling the flow rate and sample size up for the 9 × 250 mm
and 22 × 250 mm column yields essentially identical
The DNAPac PA200 is packed with a pellicular anion-exchange chromatography.
resin composed of an 8-µm-diameter nonporous polymeric
substrate, to which is bound quaternary amine-functionalized
MicroBeads. The rapid mass transport characteristics of this
Related Literature
resin result in high-resolution oligonucleotide separations. The
DNAPac PA200 can be operated under denaturing conditions, For detailed specifications and applications, see the following
such as high temperature (up to 85 °C), or high-pH eluents (up PDF documents under Literature on www.dionex.com.
to pH 12.5), or by the inclusion of chaotropic agents (such as
Urea).
Product Data Sheets
Bio Columns
The DNAPac PA200 offers the highest quality phase stabil- DNAPac PA200 Column Data Sheet
ity over a broad pH range, delivers exceptional resolution and
supports high-throughput separations. DNAPac PA200 columns
support resolution of normal-length oligonucleotides (8- to 30- Ordering Information
mer), extended-length oligonucleotides (30- to 70-mer), linear
double-stranded DNA, and supercoiled versus nicked/linear
Analytical Columns
DNA.
DNAPac PA200 Analytical (2 × 250 mm)...................................................... 063425
DNAPac PA200 Analytical Column (4 × 250 mm)......................................... 063000
Resolution of Target, Failure, and Trityl-on Oligonucleotides
80
DNAPac PA200 Analytical (9 × 250 mm)...................................................... 063421
87.5%
Full-length
DNAPac PA200 Semipreparative (22 × 250 mm).........................................SP6734
Trityl-off
Guard Columns
55.8%
DNAPac PA200 Guard (2 × 50 mm)............................................................... 063423
mA260
DNAPac PA200 Guard Column (4 × 50 mm)................................................. 062998

Failure DNAPac PA200 Guard (9 × 50 mm)............................................................... 063419
1.25 M NaCl: sequences Full-length
22.8% Trityl-off Trityl-on 22.8%
DNAPac PA200 Guard (22 × 50 mm).............................................................SP6731
0
0 8 16 24
Minutes
21022
Resolution of oligonucleotides.
www.dionex.com 263
Bio Columns
264 www.dionex.com
Amino Acid Columns
HPLC analysis of amino acids
Dionex offers two approaches to amino acid analysis: (1) anion-
exchange separation by AAA-Direct in which amino acids are
detected directly, without the need for derivatization, or (2)
anion-exchange separation with postcolumn derivatization. The
AminoPac PA10 is the recommended column for AAA-Direct,
whereas the AminoPac PA1 is used with postcolumn derivatiza-
tion.
AminoPac PA1: Pellicular, strong anion-exchange column for
• Amino Pac PA10 columns to separate free amino acids separation of amino acids in a variety of specialized applica-
without the need for derivatization tions.
• Amino Pac PA1 columns to separate free amino acids with AminoPac PA10: Hydrophobic, polymeric column stable from
derivatization pH 0–14, recommended for use with the Dionex AAA-Direct
• Compatible with solvent, high pH, and high temperatures. system.
• Provides easy scale-up.
AminoPac columns are used for high-resolution separations

of free amino acids as well as for separation and detection of a
wide range of sugars, phosphorylated amino acids, and common
oxidation products of sulfur-containing amino acids.
Bio Columns
www.dionex.com 265
Amino Acid Columns
AminoPac PA1 1 Nanomole Hydrolysate
For specialized amino acid applications with
postcolumn derivatization 12
Column:
Eluents:
AminoPac® PA1 (4 x 250 mm)
Sodium hydroxide / Sodium acetate /
1 Sodium tetraborate / Methanol
The AminoPac PA1 column is a high-speed, pellicular, strong, 13 Postcolumn
anion-exchange column. The PA1 delivers high-efficiency 14 15 17
Reagent: Ninhydrin
Reagent
7
separations as a result of its nonporous-core particle. Its strong 16 Flow Rate: 0.66 mL/min
4 9 10 11 Detection: UV/Vis, 440 and 570 nm
anion-exchange characteristics are imparted by agglomerating 3 5 Peaks: 1. Arg 10. Leu
2 6
surface MicroBead particles that are functionalized with the 8
2. Lys 11. Met
12. His
3. Thr
quaternary ammonium functional group, resulting in a higher 4. Ala 13. Phe
5. Gly 14. Glu
capacity than typically offered by nonporous resins. 0 10 20 30 40 6. Ser 15. Asp
Minutes 7. Val 16. Cys
8. Pro 17. Tyr
• Resolves phosphorylated and other amino acids by anion- 9. Ile
7658-01
exchange chromatography
• Separates acid-labile amino acids under basic pH condi- Gradient sepration of amino acid hydrolysate.
tions
• Separates strongly acidic amino acids Ordering Information
• Requires OPA or ninhydrin postcolumn derivatization
Analytical Columns
The anion-exchange selectivity of the AminoPac PA1 can be
used to resolve peaks that cannot be resolved using a cation- AminoPac PA1 Analytical Column (4 × 250 mm).......................................... 037022
exchange column. For example, O-phosphorylated amino acids
are resolved and eluted near the end of a hydrolysate chromato- Guard Columns
gram. Arogenic acid, a precursor to tyrosine and phenylalanine, AminoPac PA1 Guard Column (4 × 50 mm).................................................. 037023
decomposes under the acidic conditions required for cation-
exchange separation or PITC derivatization, but it is stable in
alkaline eluents used with AminoPac PA1 column.
The PA1 is particularly well suited for acidic and acid-labile

Bio Columns
amino acids and for amino acid pairs not completely resolved
by cation-exchange chromatography.
266 www.dionex.com
AminoPac PA10
AminoPac PA10 Determination of Amino Acids and Carbohydrates in LB Broth
Supernatant (100-fold Dilution) Using AAA-Direct Condition 30/8
For high-resolution separations of free amino acids
by AAA-Direct 1.
2.
Arginine
Arabitol
9. Alanine
10. Threonine
20. System
21. Histidine
21
0.160 3. Trehalose 20 11. Ribose 22. Glucuronic Acid
4. Lysine 23
12. Glycine 23. Phenylalanine
The AminoPac PA10 column is packed with a hydrophobic, 3
5. Glucosamine 13. Valine 24. Glutamate
6. Arabinose 14. Serine
polymeric, pellicular, anion-exchange resin that is stable over 7. Glutamine 15. Proline
25. Aspartate
26. Sulfite
the range of pH 0–14. The unique pH stability allows the use of 8. Asparagine 16. Isoleucine 27. Cystine
1 4 24 17. Leucine 28. Tyrosine
eluents that are conducive to anodic oxidation of amino acids at µC 6 8 10
5 7 1112 28
18. Methionine 29. Cysteic Acid
2 9 13 22 19. Cellobiose 30. Tryptophan
gold electrodes. This column is recommended for use with the 15 25
17 27
14
AAA-Direct Amino Acid Analyzer, allowing direct detection of 16 18 26
29 30
19
primary and secondary amino acids by IPAD, with no need for
pre- or postcolumn derivatization. 0.025
0 5 10 15 20 25 30 35 40 45 50
Minutes
19334
• Derivatization not required
• Carbohydrates and amino acids separated and detected Determination of amino acids and carbohydrates in LB broth superna-
tant.
simultaneously
• Mid-femtomole to low-picomole detection limits Standard: 2 µM all P-AA (upper trace), 8 µM* all peaks (lower trace) 1. Arginine
Column: AminoPac PA10 analytical and guard columns 2. Hydroxylysine
Inj. Volume: 30 °C 3. Lysine
• Compatible with all commonly used hydrolysis procedures Expected System
4. Glutamine
5. Asparagine
Operating Backpressure: <3,000 psi 6. Alanine
• Can be configured for on-line analysis Eluent: 7.
8.
Threonine
Glycine
E1: 18.2 megaohm-cm water
9. Valine
E2: 250 mM NaOH 10. Hydroxyproline
E3: 1 M sodium acetate
AAA-Direct is a technique that eliminates the need for any Eluent Flow Rate: 0.25 mL/min
11.
12.
Serine
Proline
17
form of derivatization, and is therefore compatible with on-line 13.
14.
Isoleucine
Leucine
15. Methionine
monitoring of cell cultures and fermentation broths. 16. Norleucine
1 17. Histidine
18. Phenylalanine
nC
2 5 21 22 19. Glutamate
18
The AminoPac PA10 column is optimized for separations of 3
4 7 10
20. Aspartate
23 20 25 26 21. Cystine
free amino acids by AAA-Direct. Vitamins, amino sugars, and 6 8 1112 15 19 24 22. Tyrosine
9 1314 16 23. P-Arginine
carbohydrates can be detected simultaneously with amino acids. 24.
25.
P-Serine
P-Threonine
Additional capabilities include separation of a wide range of 0 5 10 15
Minutes
20 25 30 26. P-Tyrosine
sugars, phosphorylated amino acids, and common oxidation
Bio Columns
*Note: Approximate concentration. Refer to the NIST SRM certificate of Analysis for the exact value of standard components 24548
products of sulfur-containing amino acids, such as cysteic acid,

Analysis of phospho-amino acids.
methionine sulfone, and methionine sulfoxide.
www.dionex.com 267
Amino Acid Columns
Determination of Amino Acids and Sugars in Wine
AminoPac PA10 - Analytical Columns

Ala
Glc Frc Ser Phe 200 pmol AminoPac PA10 Analytical Column (2 × 250 mm)........................................ 055406
Standard
Pro Tyr AminoPac PA10 (22 × 250 mm).....................................................................SP5488
Gly Met
AminoPac PA10 (9 × 250 mm).......................................................................SP5490
nC AminoPac PA10 Analytical Column (4 × 250 mm)........................................SP5678
Wine
Guard Columns
0 5 10 15 20 25 30
Minutes AminoPac PA10 Guard Column (2 × 50 mm)................................................ 055407
AminoPac PA10 Guard Column (4 × 50 mm)................................................SP5680
24549
Amino acids and sugars in red wine.

Accessories
AAA-Direct Installation Kit........................................................................... 059539
Carbohydrate Removal Cartridge (CRC) (2 x 15 mm).................................... 068598
Related Literature
Application Notes
AN 130: Identification of a Hydroxylysine-Containing Peptide

Using AAA-Direct
AN 142: Determination of Tryptophan Using AAA-Direct
AN 179: Carbohydrate and Amino Acid Analysis Using 3-D

Amperometry
Bio Columns
Technical Notes
TN 50: Determination of the Amino Acid Content of Peptides

by AAA-Direct
TN 55: Screening of Sample Matrices and Individual Matrix

Ingredients for Suitability in AAA-Direct
268 www.dionex.com
Carbohydrate Columns
HPLC analysis of carbohydrates
The CarboPac family of columns offers a selection of columns,
each optimized for a different class of compounds. Combined
with pulsed amperometric detection (PAD)—which provides
a means to detect carbohydrates without derivatization—Car-
boPac columns support simple, reliable techniques to separate
sugars.
• Sugar alcohols CarboPac MA1: High-capacity, sensitive, rugged and reliable

• Mono- and disaccharides strong anion-exchange column for separations of reduced sug-
ars, without the need for derivatization.
• Poly- and oligosaccharides
CarboPac PA1: Durable column for carbohydrate separations.
CarboPac provides high-resolution separations of glycoprotein Use for analysis of monosaccharides, disaccharides, and for
oligosaccharides and complex carbohydrates from dietary fiber, linear polysaccharide profiling.
including fructans, maltodextrins, and amylopectins; monosac- CarboPac PA10: Anion-exchange column designed for isocratic
charides, such as those obtained from glycoprotein hydrolysates separations of mono- and disaccharides with pulsed amperomet-
or plant hydrolysates, and mono- and disaccharides. ric detection.
CarboPac PA20: Fast mono- and disaccharide column. The
six common glycoprotein monosaccharides can be baseline
resolved in less than 10 min.
CarboPac PA100: Strong anion-exchange column for separa-
tion of oligosaccharides based on size, charge, branching, and
linkage.
CarboPac PA200: Recommended for high resolution separa-
tions of neutral and charged oligosaccharides. The column is
Bio Columns
used with pulsed amperometric detection.
www.dionex.com 269
CarboPac MA1 Gradient Separation of Alditols and Aldoses Potentially
CarboPac MA1 column for carbohydrate, alditol, Released by Hydrolysis of ß-Eliminated Oligosaccharides
and aldose analysis Using the CarboPac™ MA1 Column
0.16
4
The CarboPac MA1 column is a high-capacity, strong anion- 1 3
2 5 78
Column: CarboPac MA1 (4 × 250 mm)
Eluent: 100 to 850 mM Sodium hydroxide
exchange column that delivers sensitive, rugged, and reliable 6 10 12
13 nonlinear gradient
separations of reduced sugars without the need for derivatiza- 9 Detection: ED40, pulsed electrochemical
Electrode: Gold
tion. The MA1 consists of a macroporous resin fully functional- mC 11
Peaks: 1. Fucitol 8. Dulcitol
ized wth a tertiary amine. 2. GalNAcol 9. GlcNAc
3. GlcNAcol 10. Mannitol
4. Xylitol 11. GalNAc
• Recommended for the separation of reduced mono- and 5. Arabitol 12. Mannose
0 6. Fructose 13. Glucose
disaccharides 0 5 10 15 20 25 30 35 30 45
7. Sorbitol 14. Galactose
Minutes
• No derivatization needed 10261
• Separations using a simple hydroxide eluent Gradient separation of alditols and aldoses potentially released by
hydrolysis of beta eliminated oligosaccharides. Arabitol serves as an
• Alditols and aldoses separated from one another in a single internal standard.
run
Designed for reduced mono- and disaccharide alditol analyses, HPAE Separation of Sugars and Sugar Alcohols
Using the CarboPac™ MA1 Column
the CarboPac MA1 is also well suited for analysis of exogly-
0.14 3
cosidase-released neutral monosaccharides. Monosaccharides Column: CarboPac MA1 (4 × 250 mm)
are moved away from the column void, enabling immediate Eluent:
Flow Rate:
500 mM Sodium hydroxide
0.4 mL/min
evaluation of contaminating exoglycosidase activities. The Inj. Volume: 10 µL
Detection: ED40, pulsed electrochemical
MA1 can provide baseline resolution of fucose, N-acetyl- 5
Electrode: Gold
mC 2
(D)-glucosamine, N-acetyl-galactosamine, mannose, glucose, Peaks: 1.
2.
myo-Inositol
Glycerol
1 11
and galactose, as well as neutral oligosaccharides in the same 7
3. i-Erythritol
4 4. Xylitol
separation. 6 8 5. Arabitol
9 6. Sorbitol
10 7. Dulcitol
0.0 8. Mannitol
9. Glucose
10. Fructose
11. Sucrose
Bio Columns
0 10 20 30 40 50
Minutes
10744
HPAE separation of sugars and sugar alcohols without the need for
derivatization.
270 www.dionex.com
CarboPac MA1
Isocratic Separation of Alditols and Aldoses
Using the CarboPac MA1 Column
Analytical Columns
0.15 1
Column: CarboPac MA1 (4 × 250 mm) CarboPac MA1 Analytical Column (4 × 250 mm)......................................... 044066
Eluent: 0.6 M Sodium hydroxide
Detector: Pulsed amperometry,
ED40, gold electrode Guard Columns
µC
2 Peaks: 1. Inositol
3
45 2. Xylitol CarboPac MA1 Guard Column (4 × 50 mm).................................................. 044067
6 3. Sorbitol
4. Dulcitol
7 5. Mannitol
6. Glucose
0 7. Fructose
0 5 10 15 20 25
Minutes
8537
Isocratic separation of a representative set of alditols and aldoses

ensures lot-to-lot reproducibility in the manufacturing of the CarboPac
MA1 column.
Related Literature
Product Data Sheets
CarboPac MA1 Column for Carbohydrate Alditol and Aldose

Analysis Data Sheet
Application Notes
AN 87: Determination of Sugar Alcohols in Confections and
Bio Columns
Fruit Juices by HPAE-PAD

maceuticals
AN 122: The Determination of Carbohydrates, Alcohols, and

Glycols in Fermentation Broths
Technical Notes
TN 20: Analysis of Carbohydrates by High Performance Anion

Exchange Chromatography with Pulsed Amperometric Detec-
tion (HPAE-PAD)
TN 21: Optimal Settings for Pulsed Amperometric Detection of

Carbohydrates Using the Dionex ED40 Electrochemical Detec-
tor
www.dionex.com 271
CarboPac PA1 Chromatographic Analysis of Purified Inulin
Ideal column for the separation of neutral and acidic Using the CarboPac PA1 Column
monosaccharides 20
The CarboPac PA1 is an anion-exchange column for the separa- Column: CarboPac PA1
tion of mono-, disaccharides, and specific oligosaccharides Eluent: A) 0.1 M NaOH
B) 0.1 M NaOH, 1.0 M NaOAc
10 50
using an isocratic eluent. Sialic acid analysis has also been Gradient: 20–60% B in 40 min
Flow Rate: 1 mL/min
demonstrated on this column. Detection: Pulsed amperometry,
PAD, gold electrode
Range: 3000 nA
• General-purpose column for isocratic determination of 0 10 20 30 40
Sample: 0.3% Water-washed Inulin
(Polyfructose) in 0.1 M NaOH
monosaccharides and disaccharides Minutes
Sample Courtesy of Dr. C. Mitchell
California Natural Products, Manteca, CA
• Detection by pulsed amperometric detection; no derivatiza-
tion needed 3073-01
• Approved for use in a variety of official methods for the Chromatographic analysis of purified inulin.
analysis of foods
The unique MicroBead pellicular structure of the CarboPac PA1 Analysis of Mono- and Diphosphorylated Monosaccharides
on the CarboPac PA1
resin gives it stability from pH 0–14 at all concentrations of Column: CarboPac PA1
Peaks: 1. α-D-Galactosamine -1-P 1.13 µg
buffer salts. The pellicular resin structure allows excellent mass Flow Rate: 1.0 mL/min
2. α-D-Glucosamine-1-P 0.45
Detector: Pulsed amperometry, PAD, gold electrode
3. 1.75
transfer resulting in fast gradient reequilibration. Range: 10,000 nA
4.
α-D-Galactose-1-P
α-D-Glucose-1-P 1.75
Eluent A: 100 mM NaOH
Eluent B: 100 mM NaOH, 1.0 M NaOAc 5. α-D-Ribose-1-P 1.46
6. β- D-Glucose-1-P 1.75
The CarboPac PA1 column is used with pulsed amperometric Gradient: t %A %B 7 7. D-Glucosamine-6-p 3.75
8. D-Galactose-6-P 2.04
0 90 10
detection; no derivatization is required. Sensitivity on the PA1 20 80 20 12 9. D-Glucose-6-P 1.25
10. D-Fructose-1-P 0.96
is not as high as with the CarboPac PA20 or PA10 for monosac- 30 50 50
11. D-Fructose-6-P 0.42
1 12. α-D-Glucuronic acid-1-P 3.08
charide analysis. Thus the CarboPac PA1 is well suited for the 3 4
13. α-D-Glucose-1,6-Di P 1.06
2 89 11 13
analysis of foods for nutritional labeling. The CarboPac PA1 has 6
10 14 15 14. α-D-Fructose-2,6-Di P 0.92
5 15. D-Fructose-1,6-Di P 0.92
been approved for use in a number of official methods.
0 10 20 30 40
Minutes 3169
Bio Columns
Analysis of mono- and diphosphorylated monosaccharides on the

CarboPac PA1.
272 www.dionex.com
CarboPac PA1
Related Literature Ordering Information
Analytical Columns
CarboPac PA1 Analytical Column (2 × 250 mm)........................................... 057178
Product Data Sheets CarboPac PA1 Analytical Column (4 X 250 mm)........................................... 035391
CarboPac PA1 Semi-Preparative Column (9 × 250 mm)............................... 039686
CarboPac PA1 and PA10 Columns for Mono- and Disaccharide
CarboPac PA1 Semi-Preparative Column (22 × 250 mm).............................SP2866
Analysis Data Sheet
Application Notes Guard Columns

CarboPac PA1 Guard Column (2 × 50 mm)................................................... 057179
AN 61: Determination of Tobramycin and Impurities Using
HPAE-PAD CarboPac PA1 Guard Column (4 × 50 mm)................................................... 043096
CarboPac PA1 Guard (9 × 50 mm)................................................................. 063501
AN 66: Determination of Neomycin B and Impurities Using
HPAE-PAD
AN 67: Determination of Plant-Derived Neutral Oligo- and

Polysaccharides
AN 82: Analysis of Fruit Juice Adulterated with Medium Invert

Sugar from Beets
AN 92: Determination of Sugars in Molasses by High Perfor-

mance Anion Exchange with Pulsed Amperometric Detection
AN 147: Determination of Polydextrose in Foods by AOAC

Method 2000.11
AN 155: Determination of Trans-Galactooligosaccharides in

Foods by AOAC Method 2001.02
AN 186: Analysis of Paromomycin by HPAE-IPAD
Bio Columns
Application Updates
AU 150: Determination of Plant-Derived Neutral Oligo- and

Polysaccharides Using the CarboPac PA200
Technical Notes

tion (HPAE-PAD)
TN 30: Monosaccharide and Oligosaccharide Analysis of

Glycoproteins Electrotransferred onto Polyvinylidene Fluoride
(PVDF) Membranes
TN 36: Analysis of Exoglycosidase Digestions of N-Linked

Oligosaccharides Using HPAE-PAD
www.dionex.com 273
CarboPac PA10
High-Sensitivity Analysis Using the CarboPac® PA10
For sensitive analysis of mono- and disaccharides
The CarboPac PA10 is an ideal choice for separations of mono- 16.6
Column: CarboPac PA10, AminoTrap™
and disaccharides found in mammalian glycoproteins. Fucose 2
Eluent: 18 mM Sodium hydroxide
is well resolved from the system void and from protein hydro- Detection: ED40, pulsed electrochemical
1 3
lysate interferents. The CarboPac PA10 allows oxygen to elute nC 4 5
Electrode: Gold, quadruple waveform
Peaks 1. Fucose 5 pmol
after mannose, eliminating interference with monosaccharides. 6
2. Galactosamine 5
3. Glucosamine 5
It is ideal for the analysis of mono- and disaccharides in foods, 4. Galactose 5
drugs, and plants, and separates sialic acids with the addition of 5. Glucose 5
16.1 6. Mannose 5
sodium acetate to the eluent. 0 2 4 6 8 10 12
Minutes
• For the quantification of acidic, neutral, and aminomono-

13549
saccharides
• Simple, isocratic separations High-sensitivity analysis using the CarboPac PA10.
• Superior selectivity for common monosaccharides com-

pared to the CarboPac PA1 Sialic Acid Analysis of Bovine Transferrin
30 Peaks: 1. Neu5Ac
(A) 0.1 N HCI Digestion 2. KDN
• No derivatization required 3. Neu5Gc
nC 2
• Gradient compatible 3
1
0
The CarboPac PA10 is an anion-exchange column designed
specifically for high-resolution separations of mono- and disac- 30
charides. Separation is accomplished with a simple isocratic (B) Neuraminidase Digestion
eluent, and detection is accomplished with pulsed amperometric nC
2 3
detection (PAD). No derivatization is required. 1
0 5 10
Minutes
13097
Bio Columns
Sialic acid analysis of bovine transferrin on the CarboPac PA10.
274 www.dionex.com
CarboPac PA10
Determination of Glycols, Sugar Alcohols, and Carbohydrates
Using the CarboPac PA10 Column
Analytical Columns
CarboPac PA10 Analytical Column (2 × 250 mm)......................................... 057180
4 Column: CarboPac PA10, PA10 guard
120 3 Eluent: 18 mM Sodium hydroxide CarboPac PA10 Analytical Column (4 × 250 mm)......................................... 046110
Inj Volume: 10 µL CarboPac PA10 Semipreparative (9 × 250 mm)............................................SP4216
Detection: Pulsed amperometry,
2
nC ED40, gold electrode
5 6 Peaks: 1. Propylene glycol
1 7 2. Glycerol Guard Columns
3. Sorbitol
0
4. Mannitol CarboPac PA10 Guard Column (2 × 50 mm)................................................. 057181
5. Maltitol
0 2 4 6 8 10 12 14 6. Glucose AminoTrap Column (2 × 50 mm)....................................................................SP5578
Minutes 7. Sucrose
Used as a guard column with the CarboPac PA10 when analyzing samples
13155 containing amino acids.
CarboPac PA10 Guard Column (4 × 50 mm)................................................. 046115
The CarboPac PA10 resolves mixtures of glycols, sugar alcohols, and
carbohydrates in a pharmaceutical formulation. Amino Trap Column (4 × 50 mm)................................................................... 046122
Use as a guard column with the CarboPac PA10 when analyzing samples
containing amino acids.
Related Literature Borate Trap Column....................................................................................... 047078
Use as an eluent trap column with the CarboPac PA10 for removal of borate
For detailed specifications and applications, see the following contamination from the eluent.
PDF documents under Literature on www.dionex.com. Amino Trap Column (3 × 30 mm)................................................................... 060146
Use as a guard column with the CarboPac PA10 when analyzing samples
Product Data Sheets containing amino acids.
CarboPac PA1 and PA10 Columns for Mono- and Disaccharide

Analysis Data Sheet
Application Notes
Bio Columns
maceuticals
Application Updates
AU 141: Improved Long-Term Stability of N-Acetylneuraminic

Acid and N-Glycolylneuraminic Acid Peak Area Responses Us-
ing Waveform A, a Quadruple Potential Waveform
Technical Notes

tion (HPAE-PAD)
TN 40: Glycoprotein Monosaccharide Analysis Using High-

Performance Anion-Exchange Chromatography with Pulsed
Amperometric Detection (HPAE-PAD)
TN 41: Analysis of Sialic Acids Using High-Performance

Anion-Exchange Chromatography
www.dionex.com 275
CarboPac PA20 Analysis of Chocolate Syrup Using the CarboPac® PA20
Fast, sensitive mono- and disaccharide analysis
60
The CarboPac PA20 is a strong anion-exchange column that 1 Column: CarboPac PA20 and guard, 3-mm
Eluent: 50 mM NaOH
provides high-resolution separations of mono- and disaccha- Flow Rate: 0.5 mL/min
Temperature: 30 ˚C
rides with no need for derivatization. This column provides Detection: Pulsed electrochemical, Au electrode
Waveform: Quadruple potential
baseline resolution between the six monosaccharides commonly nC
Sample: 1:10,000 dilution of chocolate syrup
2 3
found in mammalian glycoproteins over a wide range of eluent Peaks: 1. Glucose
2. Fructose
concentrations. The CarboPac PA20 provides slightly different 4 5
3. Sucrose
4. Unidentified
selectivity from either the CarboPac PA1 or PA10, making it an 0 5. Unidentified
0 2 4 6 8 10 12 14 16 18 20
attractive choice for separating difficult peak pairs. Minutes
• The column of choice for high-sensitivity monosaccharide

analyses 18873
• Highly efficient separations with good resolution between Analysis of chocolate syrup using the CarboPac PA20.
neighboring peaks
• Fast analysis—less than 10 min Sialic Acid Analysis of Bovine Fetuin
• Optimized resolution of glucosamine/galactose and glu- 400

cose/mannose peak pairs NANA
Column: CarboPac® PA20 (3 × 150 mm)
• Lower eluent consumption Gradient: 20–200 mM NaOAc
in 0.1 M NaOH over 10 minutes
• Does not require derivatization nC
NGNA A Detection: Pulsed electrochemical,
disposable Au electrode
NGNA Waveform: Quadruple potential
The CarboPac PA20 consists of 6.5-µm-diameter nonporous NANA Samples: A. 0.1 M HCl fetuin hydrolysate
B (10 mg injected, 80 °C, 1 h)
beads covered with a fine latex of functionalized MicroBead B. NANA and NGNA standard
0
resin. This pellicular resin structure permits excellent mass
0 2 4 6 8 10 12 14 16 18 20
transfer, resulting in high-resolution chromatography and rapid Minutes
reequilibration. The performance of each column is verified

18872
with a Dionex MonoStandard.
Bio Columns
Sialic acid analysis of bovine fetuin.
276 www.dionex.com
CarboPac PA20
Separation of Monosaccharides Using the
CarboPac PA20 without an AminoTrap
Analytical Columns
2
1 System: ICS-3000
3
5 Column: CarboPac® PA20 (3 x 150 mm)
CarboPac PA20 Analytical Column (3 × 150 mm)......................................... 060142
4 6
Eluent: 8–20 mM KOH (Eluent Generator)
20 mM KOH Temperature: 30 °C
18 mM
Flow Rate: 0.5 mL/min Guard Columns
Inj. Volume: 10 µL
nC 16 mM Detection: PAD, disposable Au
working electrode CarboPac PA20 Guard Column (3 × 30 mm)................................................. 060144
14 mM
Peaks: 1. Fucose 100 pmol
12 mM 2. Galactosamine 100
10 mM 3. Glucosamine 100
4. Galactose 100
8 mM 5. Glucose 100
6. Mannose 100
0 8 10 15
Minutes
21556-01
Effect of hydroxide concentration on elution times.
Related Literature
Product Data Sheets
CarboPac PA20 Column Data Sheet
Bio Columns
www.dionex.com 277
CarboPac PA100
Separation of Neutral Oligosaccharide Standards
Low-picomole oligosaccharide analysis
Column: CarboPac® PA100 + Guard
40 Eluent: 0 to 80 mM Sodium acetate
Oligosaccharides released from therapeutic glycoproteins can 2 gradient in 250 mM Sodium hydroxide
Flow Rate: 1 mL/min
differ in size, charge, branching, and linkage. Oligosaccharides Inj. Volume: 25 µL
with these features can be resolved using the CarboPac PA100. 1
9 12
Detection: PAD (Gold)
5 7 11 Peaks: 1. fucosylated Man3GlcNAc2
It provides the flexibility to separate oligosaccharides into broad nC
4
8 2. Man3GlcNAc2
3 10 3. asialo agalacto bi, core fuc
classes depending upon the degree of sialylation, while main- 6
4. asialo agalacto bi
taining the resolution needed for routine analysis based on link- 5.
6.
Man5GlcNAc2
asialo agalacto tri
age isomerism. Coupled with pulsed amperometric detection, 0 7. asialo bi, core fuc
8. asialo bi
the PA100 supports detection at low-picomole levels. 0 5 10 15 20 25 9. asialo tri
Minutes
10. bisected hybrid
11. asialo tetra
• Separation of closely related oligosaccharides, even iso- 12. Man9GlcNAc2
9908-01
mers, at picomole levels
• Separation of neutral and charged oligosaccharides in the Separation of neutral oligosaccharide standards. Twelve commonly
same run occurring N-linked neutral oligosaccharides are easily resolved within
20 minutes.
• No sample derivatization required for detection
Separation of Neutral and Sialylated Oligosaccharide Standards
The CarboPac PA100 column is a high-resolution, strong anion-
Using the CarboPac® PA100 Column
exchange column for enhanced chromatography of oligosac-
charides. Column: CarboPac PA100
Eluent: 0–250 mM Sodium acetate
over 110 min in 100 mM
Net Negative Charge Sodium hydroxide
The Carbohydrate Membrane Desalter can be used on-line prior 200 2 Flow Rate: 1 mL/min
1 Detection: Pulsed amperometry,
to fraction collection. 0 I II III IV PAD, gold electrode
nA 5 11 Peaks: 1. Fucosylated Man3GlcNAc2

4 10 2. Man3GlcNAc2
7 3. Asialo agalacto bi, core fuc
6 13
3
89 12 4. Asialo agalacto bi
20 5. Asialo bi, core fuc
6. Asialo bi
0 10 20 30 40 50 60 70 7. Man9GlcNAc2
Minutes 8, 9. Disialylated tri (reduced)
10, 11. Trisialylated tri (reduced)
12, 13. Tetrasialylated tri (reduced)
Bio Columns
10318
Separation of neutral and sialylated oligosaccharide standards.
278 www.dionex.com
CarboPac PA100
Separation of Trisialylated Oligosaccharides Ordering Information

Using the CarboPac™ PA100 Column
120
2 Column: CarboPac PA100
Eluent: 0–250 mM Sodium acetate Analytical Columns
1 over 110 min in 100 mM
Sodium hydroxide CarboPac PA100 Analytical Column (2 × 250 mm)....................................... 057182
nA Flow Rate: 1 mL/min
Detector: Pulsed amperometry, CarboPac PA100 Analytical Column (4 × 250 mm)....................................... 043055
PAD, gold electrode
CarboPac PA100 Column (9 × 250 mm)........................................................SP2089
20 CarboPac PA100 Column (22 × 250 mm)......................................................SP2667
40 45 50 55 60 65 70
Minutes
NANA
α2-6
Gal
β1-4
GlcNAc
β1-2
Man α1-6 NANA Gal
α2-3 β1-4
GlcNAc
β1-2
Man α1-6 Guard Columns
Man GlcNAc GlcNAc Man GlcNAc GlcNAc
β1-4 β1-4 β1-4 β1-4
NANA Gal GlcNAc Man α1-3 NANA Gal GlcNAc Man α1-3 CarboPac PA100 Guard Column (2 × 50 mm)............................................... 057183
α2-6 β1-4 β1-2 α2-6 β1-4 β1-2
NANA Gal GlcNAc

β1-4
Peak 1 NANA Gal GlcNAc
β1-4
Peak 2 CarboPac PA100 Guard Column (4 × 50 mm)............................................... 043054
α2-3 β1-4 α2-3 β1-4
10323
Separation of trisialylated oligosaccharides.
Related Literature
Product Data Sheets
CarboPac PA100 Column for Oligosaccharide Analysis Data

Sheet
Application Notes

Analysis of Beer
Bio Columns
AN 67: Determination of Plant-Derived Neutral Oligo- and
Polysaccharides

Sugar from Beets
AN 105: Glycosylation Analysis of Human Serum Transferrin

Glycoforms Using Pellicular Anion-Exchange Chromatography
Technical Notes

tion (HPAE-PAD)
TN 30: Monosaccharide and Oligosaccharide Analysis of

Glycoproteins Electrotransferred onto Polyvinylidene Fluoride
(PVDF) Membranes
TN 36: Analysis of Exoglycosidase Digestions of N-Linked

Oligosaccharides Using HPAE-PAD
TN 42: Glycoprotein Oligosaccharide Analysis Using High-

Performance Anion-Exchange Chromatography
www.dionex.com 279
CarboPac PA200 Faster Separation of Maltodextrins with the CarboPac® PA200 Column
Fast, pH-stable separation of oligosaccharides
1
0.5 3
A variety of HPLC approaches are proposed for characterizing Column: CarboPac PA200 + guard
Eluent: 0 min: 100 mM NaOH/
oligosaccharides, but many prove inadequate for separating 5 70 mM sodium acetate,
complex mixtures and are limited by a lack of specificity and 10 30 min: 100 mM NaOH/
300 mM sodium acetate, curve 4
high limits of detection. Dionex has developed an improved µC
Temperature: 30 °C
HPLC technique for carbohydrate analysis based on anion- Inj. Volume: 5 µL
exchange chromatography coupled with pulsed amperometric 20 Detection: PAD (Au), waveform A (TN21)
Sample: Maltrin M040 5 mg/mL in water
detection. This technique provides high-resolution separations 30 40
Peaks: 3. Maltotriose —
with sensitive and specific detection, without derivatization.
0
0 5 10 15 20 25 30 35
• Sensitive, direct detection with no derivatization, using Minutes
22682
pulsed amperometric detection
• Very high-efficiency separations Separation of maltodextrins with the CarboPacPA200 column.
• Separations based on size, charge, degree of branching, and

linkage isomerism Related Literature
• Low flow rate saves on eluent consumption
• Does not require derivatization PDF documents under Literature on www.dionex.com.
CarboPac PA200 columns are packed with a hydrophobic,

Product Data Sheets
polymeric, pellicular anion-exchange resin stable over the range
of pH 0–14. The unique pH-stability of the packing material al- CarboPac PA200 Column Solutions for Oligosaccharide Analy-
lows the use of eluent compositions that are conducive to anodic sis Data Sheet
oxidation of carbohydrates at gold electrodes.
Application Updates
AU 150: Determination of Plant-Derived Neutral Oligo- and

Polysaccharides Using the CarboPac PA200
Bio Columns
Guard Columns
CarboPac PA200 Guard Column (3 × 50 mm)............................................... 062895
Analytical Columns
CarboPac PA200 Analytical Column (3 × 250 mm)....................................... 062896
280 www.dionex.com
Chromatography Accessories
Chemical Suppressors 283 CRD Carbonate Removal Device....................... 292

SRS 300 Self-Regenerating Suppressor........... 284 Ordering Information........................................................ 293
Ordering Information........................................................ 284 OnGuard II Cartridges......................................... 294
Atlas Electrolytic Suppressor............................. 285 OnGuard II A....................................................................... 294
Related Literature.............................................................. 285 OnGuard II Ag.................................................................... 294
Ordering Information........................................................ 285 OnGuard II Ag/H................................................................ 294
OnGuard II Ba.................................................................... 295
AMMS-ICE 300 MicroMembrane Suppressor.. 286 OnGuard II Ba/Ag/H.......................................................... 295
Related Literature.............................................................. 286 OnGuard II H....................................................................... 295
Ordering Information........................................................ 286 OnGuard II M...................................................................... 295
OnGuard II Na.................................................................... 296
MMS 300 MicroMembrane Suppressor........... 287 OnGuard II P....................................................................... 296
Related Literature.............................................................. 287 OnGuard II RP.................................................................... 296
Ordering Information........................................................ 287 OnGuard Accessories...................................................... 296
SC-CSRS Salt Converter..................................... 288 Standards, Reagents, and Eluent

Concentrates........................................................ 297
Ion Standard Concentrates............................................. 297
CMD 300 Carbohydrate Membrane Desalter... 288 MS Standard Concentrates............................................ 297
OQ/PQ Standards.............................................................. 297
Anion Eluent Concentrates............................................. 297
Cation Eluent Concentrates and Reagents.................. 298
Displacement Chemical Regen. Reagents................... 298
MMS Regenerant Concentrates.................................... 298
Supplies & Accessories 289
Ion Pairing Reagents........................................................ 298
Transition Metal Analysis Reagents.............................. 298
RFIC-Eluent Generation...................................... 290
Chelation Chromatography Reagents........................... 298
Carbohydrate Standards................................................. 298
AAA-Direct Reagents....................................................... 298
RFIC-Eluent Regeneration.................................. 290
ICS-900 Solutions Kits........................................ 299
AS4A 4 mm Bundled Package........................................ 299
AS9-HC 4 mm Bundled Package.................................... 299
AS12A 4 mm Bundled Package...................................... 299
CR-TC Continuously-Regenerated Trap AS14 4 mm Bundled Package......................................... 299
Column................................................................. 291 AS14A 4 mm Bundled Package...................................... 300
Related Literature.............................................................. 291 AS14A 3 mm Bundled Package...................................... 300
Ordering Information........................................................ 291 AS22 4 mm Bundled Package......................................... 300
AS23 4 mm Bundled Package......................................... 300
CS12A 4 mm Bundled Package...................................... 300
CS12A 3 mm Bundled Package...................................... 301
CS16 5 mm Bundled Package......................................... 301
CS16 3 mm Bundled Package......................................... 301
LCi Solutions Kits................................................ 302

General LCi Solutions Kits............................................... 302
LCi Solutions Kits for Proteomics................................... 303
www.dionex.com 281

282
www.dionex.com
Chemical Suppressors
Improving sensitivity by reducing eluent conductivity
and enhancing analyte response
Suppression works two ways to achieve the absolute best sen-
sitivity and corresponding lowest detection limits for inorganic
analyses; it increases analyte signal while simultaneously de-
creasing background signal and noise. Dionex was the first com-
pany to commercialize chemical suppression in 1975, and has
continued to expand the capabilities of suppression technology
by inventing electrolytic suppression in 1992. Today we offer a SRS 300 Self-Regenerating Suppressor: The latest electrolytic
full line of choices tailored to meet the needs of our customers. suppressor. This suppressor is a true workhorse, serving virtu-
ally all IC applications.
• SRS 300 Self-Regenerating Suppressor
Atlas Electrolytic Suppressor: Continuous electrolytically-re-
• Atlas Electolytic Supressor generated suppressor based on Dionex MonoDisc and AutoSup-
pression products.
• MMS 300 MicroMembrane Suppressor
AMMS-ICE 300 MicroMembrane Suppressor: Ideally suited to
• AMMS-ICE 300 MicroMembrane Suppressor for Ion
ion-exclusion chromatography of organic acids and alcohols in
Exclusion Chromatography
complex or high-ionic-strength samples.
• SC-CSRS 300 Salt Converter Cation Self-Regenerating
MMS 300 MicroMembrane Suppressor: High-capacity, solvent-
Suppressor
compatible, chemically-regenerated suppressor.
• CMD 300 Carbohydrate Membrane Desalter
SC-CSRS Salt Converter: Optimized to convert analytes to the
Electrolytic suppression based on Reagent-Free Ion Chroma- fully dissociated methanesulfonic acid species.
tography (RFIC) with AutoSuppression is available only from CMD 300 Carbohydrate Membrane Desalter: Optimized to
Dionex. remove eluent salts from high-pH eluents.
www.dionex.com 283
SRS 300 Self-Regenerating
Suppressor
High-capacity electrolytically-regenerated
suppressor for use with IC eluents.
The SRS 300 serves virtually all ion chromatography applica-
tions for both anions and cations. For anions, the preferred elu-
ent is hydroxide. With RFIC systems, the sample is determined
using conductivity detection in the lowest possible background
of high-purity water. AutoSuppression means ease of use; you
don’t need to make regenerant because the suppressor is con-
stantly regenerated by the continuous electrolysis of water. The
SRS 300 offers:
• Low background noise levels

• Fast start-up equilibration times
Related Literature
• Trace anion and cation determinations
• Compatible with mass spectrometry detection PDF documents under Literature on www.dionex.com.
• Compatible with all Dionex ICS and DX chromatography
modules* Product Data Sheets
• A three-fold increase in backpressure tolerance compared Eluent Suppressors for Ion Chromatography Data Sheet
to previous generations
The SRS 300 delivers low backgrounds and low noise levels Ordering Information
for the full range of Dionex ion-exchange IonPac columns and
eluents, even under high concentration gradient conditions. The SRS 300 is compatible with Dionex IC systems equipped
with a suppressor power supply. The new SRS 300 products
When combined with the revolutionary Eluent Generator, it directly replace the SRS ULTRA II. Simply install the new SRS
delivers truly automated, minimal-maintenance Reagent Free 300 into your current Dionex IC system.
Ion Chromatography (RFIC).
Accessories
Note: *Chemical suppression mode only with the ICS-90 and
ICS-900 systems. Older systems may require the RFC-10 or Anion Self-Regenerating Suppressor ASRS 300 4 mm............................... 064554
RFC-30 controller. Anion Self-Regenerating Suppressor ASRS 300 2 mm............................... 064555
Cation Self-Regenerating Suppressor CSRS 300 4 mm.............................. 064556
Cation Self-Regenerating Suppressor CSRS 300 2 mm.............................. 064557
284 www.dionex.com
Atlas Electrolytic Suppressor
Atlas Electrolytic Suppressor Ordering Information
Low noise electrolytically regenerated suppressor for The Atlas suppressor is fully compatible with the ICS-3000,
use with IC eluents ICS-2000, ICS-1500, and ICS-1000 chromatography systems.
It is not compatible with the ICS-90 or ICS-900. When ordering
The Atlas Electrolytic Suppressor (AES) is a continuous an Atlas suppressor for older systems, the DX-600, BioLC, or
electrolytically-regenerated suppressor based on the Mono- DX-320 systems must be configured with an ED50A, CD25A,
Disc and AutoSuppression products developed by Dionex. The or IC25A detector. The RFC-30 or RFC-10 Suppressor Control-
unique design of the Atlas enables very fast startup times and ler must be ordered to use the Atlas with the earlier versions of
an extremely-low noise level, particularly for carbonate eluents. detectors.
The benefits of the Atlas include:
• High performance and very low noise for carbonate eluents Accessories
• Low background conductivity, and excellent baseline Anion Atlas Electrolytic Suppressor............................................................. 056116
stability Cation Atlas Electrolytic Suppressor............................................................ 056118
• Easy to use maintenance-free operation
• Fast startup and equilibration
• Will suppress eluents up to 25 µeq/min
The MonoDisc suppression bed of the AES is composed of ion-

exchange monolith and flow distribution disks. This configura-
tion facilitates efficient exchange of the eluent counter ions for
regenerant ions, resulting in eluent suppression and analyte
response enhancement.
Related Literature Chromatography Accessories

Product Data Sheets
Eluent Suppressors for Ion Chromatography Data Sheet
www.dionex.com 285
AMMS-ICE 300 MicroMembrane Related Literature
Suppressor For detailed specifications and applications, see the following
Chemically-regenerated suppressor optimized for PDF documents under Literature on www.dionex.com.
ion-exclusion chromatography
Product Data Sheets
The Anion-ICE MicroMembrane Suppressor AMMS-ICE 300
is designed for the analysis of organic acids and alcohols in Eluent Suppressors for Ion Chromatography Data Sheet
complex or high-ionic-strength samples, including food and
beverage products, biological samples, fermentation processes,
industrial process liquors, and treated wastewaters. This sup- Ordering Information
pressor is ideally suited to ion-exclusion chromatography of
organic acids and alcohols in complex or high-ionic-strength Accessories
samples. It provides the following advantages:
Anion-ICE MicroMembrane Suppressor AMMS-ICE 300 4 mm................. 067527
• High-capacity and solvent compatible design
• Optimized for organic acid and alcohol analysis in complex
or high-ionic-strength samples
• Low detection limits, high signal-to-noise ratio, and
excellent baseline stability
• Fast startup and equilibration
The AMMS-ICE 300 suppressor is used in chemical suppres-

sion mode with a tetrabutylammonium hydroxide (TBAOH)
regenerant. Standard ion suppression converts analytes into
their acid or base forms. The AMMS-300 suppressor increases
analyte conductivity by forming the TBA salt of the weak acid
analyte, which is more conductive than the partially ionized acid
form of the analyte.
The AMMS-ICE 300 removes the hydronium ion and any coun-
terions of the analytes from the eluent stream and replaces them
with tetrabutylammonium ion. This removal greatly reduces
background conductivity. In addition, this mechanism forces the
ionization of weak acid analytes, increasing sensitivity.
286 www.dionex.com
MMS 300 MicroMembrane Suppressor
MMS 300 MicroMembrane
Suppressor
High-capacity, solvent-compatible, chemically-
regenerated suppressor
The MMS 300 is a chemically-regenerated suppressor and
therefore has very low noise and quick start-up time. With the
new Displacement Chemical Regeneration (DCR) Kit, this
classic suppressor provides easier operation. MMS Suppressor
advantages include:
• High-capacity and solvent-compatible design capable of

handling samples with complex matrices
• Very low signal-to-noise and excellent baseline stability
• Low method detection limits
Related Literature
• Fast start-up and equilibration
• Easy to use, maintenance-free operation with the DCR kit
The MMS 300 has high capacity and can be used with all Dion-
ex ion-exchange columns. For lowest noise and longest life, the Product Data Sheets
MMS is recommended for anion and cation separations using
eluents containing HPLC solvents. The MMS 300 is available in Eluent Suppressors for Ion Chromatography Data Sheet
both 2- and 4 mm formats; 3 mm columns should be used with
2 mm suppressors.
The MMS produces the lowest noise because it uses nonelec-
trolytic chemical regeneration. Low noise levels translate into Accessories
lower method detection limits. Anion MicroMembrane Suppressor AMMS 300 4 mm............................... 064558
Anion MicroMembrane Suppressor AMMS 300 2 mm............................... 064559
Cation MicroMembrane Suppressor CMMS 300 4 mm.............................. 064560
Cation MicroMembrane Suppressor CMMS 300 2 mm.............................. 064561
www.dionex.com 287
SC-CSRS Salt Converter CMD 300 Carbohydrate
Converts analytes to the fully-dissociated Membrane Desalter
methanesulfonic acid species On-line device for users of HPAE-PAD to collect and
further analyze carbohydrate samples
With the SC-CSRS 300, nonlinear response of ammonia and
certain amines can be overcome by converting the weak base High-pH HPAE-PAD has become the method of choice for
analyte to a fully ionized form, thus extending the linear re- oligosaccharide purification because it permits very high resolu-
sponse. The SC-CSRS 300 suppressor is designed to make this tion of neutral and charged oligosaccharide isomers. Subsequent
conversion and provide linearity across as much as three orders characterization of purified oligosaccharides often requires
of magnitude. removal of eluent salts. Manual desalting methods are available;
the CMD 300 provides an easier, automated method.
• Increased linearity for ammonium and amines
• Increased sensitivity for ammonium and amines • Reduces the pH of the effluent from pH ~13 to between pH
2–6
• Low background and noise
• Supports lyophilization of collected fractions without
• Conversion to and detection as fully dissociated methane- dialysis
sulfonic acid
• Provides minimal dispersion of the sample exiting the
The SC-CSRS should be used in industries where regulated detector cell
methods require a linear response. A linear response increases
• Low dispersion for accurate fraction collection
accuracy at higher concentrations, and so requires fewer cali-
bration check standards over the calibration curve. Reduced • >99% sodium ions removed from eluents that contain up to
calibration requirements increase sample throughput for indus- 0.35 M sodium ions
tries with high sample workloads such as the power generation
industry. The CMD 300 exchanges sodium ions in the eluent for hydro-
nium ions. This process converts sodium hydroxide and sodium
Method detection limits for Group I and II cations, ammonium, acetate eluents to water and acetic acid, lowering sample pH
and amines using the SC-CSRS are equivalent to or better than after samples leave the detector cell. Because acetic acid is vola-
those achieved using the CSRS 300. tile, fractions can then be lyophilized without dialysis, leaving
the purified carbohydrate sample ready for further manipulation.
The CMD 300 reliably desalts up to 0.35 M Na at an eluent flow

Related Literature
of 1.0 mL/min. Oligosaccharides eluting in up to 0.35 M Na,
For detailed specifications and applications, see the following collected after on-line desalting and evaporated in a centrifugal
PDF documents under Literature on www.dionex.com. vacuum evaporator, exhibit residual [Na+] below 200 µM after
resuspension to original volume in deionized water. This repre-
sents a desalting efficiency of >99.9%.
Product Data Sheets
Salt Converter-Cation Self-Regenerating Suppressor 300

Accessories
Accessories Carbohydrate Membrane Desalter CMD 300, 4 mm................................... 067528

CMD 300 Start-up Package........................................................................... 059091
Salt Converter-Cation Self-Regenerating Suppressor, SC-CSRS 300
4 mm.............................................................................................................. 067530
Salt Converter-Cation Self-Regenerating Suppressor, SC-CSRS 300
2 mm.............................................................................................................. 067529
288 www.dionex.com
Supplies & Accessories
Column hardware, RFIC accessories, standards,
reagents, and cartridges
RFIC-Eluent Generation: Eluent generator cartridges for the

electrolytic production of high-purity eluents for isocratic and
gradient runs.
RFIC-Eluent Regeneration: Allows non-stop operation of a
carbonate or methanesulfonic-acid eluent applications for up to
4 weeks at a time.
CR-TC: Continuously Regenerated Trap Columns (CR-TC) for
Reagent-Free Ion Chromatography (RFIC-EG).
CRD Carbonate Removal Device: The CRD causes carbon
dioxide to diffuse through the walls of a permeable membrane,
removing it prior to detection or injection.
OnGuard II Cartridges: Remove matrix interferences such as
phenolics, metals, cations, anions, or hydrophobic substances,
for better performance in many IC applications.
Reagents: A complete line of standards, reagents, and eluent
concentrates for ion chromatography applications.
ICS-900 Consumables Packages: Bundled kits with matched
columns, suppressor, and application-specific supplies recom-
mended for the ICS-900 and similar systems.
LCi Solutions Kits: Preconfigured capillary kits and comprehen-
sive quick installation guides for fast and convenient implemen-
tation of UltiMate 3000 LCi Solutions.
www.dionex.com 289
RFIC-Eluent Generation RFIC-Eluent Regeneration
Eluent Generation—a major component of Reagent- Allows non-stop operation of an IC system for up to
Free IC four weeks at a time
Eluent generation prevents baseline shift, increases sensitivity, RFIC-ER is a low-cost alternative to RFIC-EG systems. Rather
improves resolution, and ensures consistent peak integration. than generate eluent electrolytically using deionized water,
Dionex offers a range of EluGen cartridges for the production of RFIC-ER uses the electrolytic suppressor to regenerate the start-
hydroxide, carbonate, and methanesulfonic acid eluents. Eluent ing eluent. Eluent is prepared in the normal manner. After use
generation eliminates the need to handle acids and bases tradi- and regeneration, the eluent is passed through a series of eluent
tionally required for the preparation of IC eluents, and allows purifier columns to produce pure eluent. The purified eluent is
chromatographers to run a full range of gradient and isocratic returned to the eluent bottle. One batch of original eluent can
separations more effectively than hand-made eluents. provide up to four weeks of non-stop operation.
• Automatic production of high-purity eluents for isocratic • Always on, always ready capability for ICS-1000, -1500,
and gradient runs on ICS-3000 and ICS-2000 systems -2000, and -3000 systems
• Outstanding run-to-run reproducibility week after week • System remains fully equilibrated and calibrated for long
periods of time
• Elimination of acid and base handling
• Simple plumbing and easy-to-understand schematic
• Longer lasting pumps because they encounter only deion-
ized water • Low cost of ownership
EluGen cartridges support a concentration range of 0.1– • Ideal for the analysis of common anions and cations in
100 mM (0.1–80 mM EGC-LiOH) at 1.0 mL/min. The KOH, drinking, surface and ground water samples
NaOH and LiOH cartridges support a maximum of 25% • Operator errors are significantly reduced or eliminated
methanol. The potassium carbonate and MSA cartridges are not
solvent-compatible.
Related Literature
Related Literature For detailed specifications and applications, see the following

Product Brochures
Product Brochures Reagent-Free Ion Chromatography
Reagent-Free Ion Chromatography Product Data Sheets
Product Data Sheets Reagent-Free Ion Chromatography Systems with Eluent Regen-
eration Data Sheet
Reagent-Free Ion Chromatography Systems with Eluent Gen-
eration for IC Without Manually Prepared Eluents
Accessories
EluGen II KOH Cartridge (Replaces 053921)................................................. 058900
EluGen II NaOH Cartridge............................................................................. 058908
EluGen II LiOH Cartridge................................................................................ 058906
EluGen II K2CO3 Cartridge (order EPM for CO3:HCO3 Eluent Generation).... 058904
EPM Electrolytic pH Modifier (needed to generate CO3:HCO3 Eluent)........ 063175
EluGen II MSA Cartridge (Replaces 053922)................................................ 058902
290 www.dionex.com
RFIC-Eluent Generation
Ordering Information CR-TC Continuously-Regenerated
Startup kits include the tubing, controllers, and software
Trap Column
needed to upgrade an ICS-1000, -1500, -2000, or -3000 system Reduces baseline drift by removing contaminants
to an RFIC-ER system, in addition to one complete set of
consumables. Designed for eluent generators in RFIC systems, CR-TCs
remove all anionic or cationic contaminants in the eluent con-
tinuously and provide very low baseline drift during gradient
RFIC-ER operations. The CR-TC offers:
• Contaminant-free deionized source water and eluent
Includes one Anion Consumables Kit
RFIC-ER Cation Startup Kit............................................................................ 067798 • Time savings—no need to perform the regeneration off-line
Includes one Cation Consumables Kit • Very low baseline drift for improved integration and in-
creased sensitivity
Accessories
• Increased productivity; quality data soon after start-up
RFIC-ER Anion Consumables Kit................................................................... 067791
• Sample pretreatment with the CR-CTC II
RFIC-ER Cation Consumables Kit.................................................................. 067792
Plumb the CR-TC after the EG Cartridge (EGC), removes
eluent contaminants continuously and and achieve very low
baseline drift during gradient operations.
Related Literature
Product Data Sheets
CR-TC
CR-ATC Continuously Regenerated Anion Trap Column.............................. 060477
CR-CTC II Continuously Regenerated Cation Trap Column.......................... 066262
www.dionex.com 291
CRD Carbonate Removal Device 1.80
6 7 Column: IonPac® AS19, 4 mm
Reduces carbonate for improved sensitivity Without Suppressor:
Current:
ASRS ULTRA II, 4 mm
300 mA
CRD 200
Loop: 200 µL
µS
Oven: 30 °C
The CRD causes carbon dioxide in the suppressed eluent stream 1 5 8 Time: Gradient (mM)
3 4 6
to diffuse through the walls of a gas permeable membrane. The 2 0
10
10
10
net effect is a reduction in the response from carbonate. It there- 0.40 25 45
30 45
fore removes the carbonate peak in hydroxide eluent RFIC-EG 7 30.1 10
1.80 35 10
systems or reduces the background to near hydroxide-like levels 6
7 Peaks: Concentration (ppb)
1. Fluoride 10
in carbonate eluent systems. Plumbed after the suppressor, the With 2. Chloride 15
carbon dioxide transfer is aided by a counter-current flow of µS CRD 200 3. Nitrite
4. Bromide
50
50
8
basic solution. 1
2 3 4
5 5. Nitrate 50
6 6. Carbonate –
7. Sulfate 75
0.40
• The CRD 200 is optimized for the removal of the carbonate 0 5 10 15 20 25 30 35
8. Phosphate 75
Minutes
in hydroxide eluent systems. 20557-01
• Minimizing carbonate using the CRD 200 can improve Decreased carbonate peak with the CRD 200.
quantification.
• The increased membrane length of the CRD 300 is opti- 26.00
No CRD 300
5 Column: IonPac AS9-HC
mized for carbonate eluent systems. 1
2 3 Eluent: 9.0 mM Carbonate
• Reducing the baseline with a CRD 300 results in higher µS Inj. Volume:
Temperature:
25 µL
30 °C
sensitivity. Detection: ASRS ULTRA II, 4 mm
AutoSuppression
21.00 recycle mode, 59 mA
• Elimination of the carbonic acid matrix with a CRD 300
Peaks: 1. Fluoride 1.0 mg/L
increases the linearity range. 2. Chloride 1.5
CRD 300
4.50 5 3. Nitrate 5.0
1 4. Phosphate 7.5
3
By simply being exposed to carbon dioxide in the air, samples 2 5. Sulfate 7.5
can become contaminated with carbonate. In some samples, de- µS 4 Background: w/o CRD 300 23.0 µS/cm
pending on the column and separation conditions, the presence w/ CRD 300 0.87 µS/cm
of high levels of carbonate originating from dissolved carbon 0.50

dioxide interferes with the accurate determination of analytes of 0 5 10 15 20
Minutes 23606
interest, such as sulfate and nitrite.
Reduced background with the CRD 300.

The CRD 200 can be installed easily in any Dionex IC system
equipped with an eluent generator, CR-ATC, and ASRS 300 The CRD 300 is easily installed in any Dionex IC system
or ULTRA II suppressor. The CRD 200 requires no reagents equipped with an ASRS 300 suppressor. It requires either a
or software control. The CRD 200 is a low-dispersion device constant flow of basic solution from a peristaltic pump or evacu-
designed specifically for use with hydroxide-based and borate- ation by a vacuum pump. It is a low-dispersion device designed
based chemistries using Dionex columns. for use with carbonate-based chemistries using Dionex columns.
Note: The CRD 300 is not recommended for use with an

ASRS ULTRA II suppressor. Related Literature
Product Data Sheets
Carbonate Removal Device (CRD) 200 for RFIC Systems
Carbonate Removal Device 300 (CRD 300) for Carbonate

Eluents Data Sheet
292 www.dionex.com
CRD Carbonate Removal Device
CRD 200
Carbonate Removal Device CRD 200 (4 mm)............................................... 062983
CRD 300
CRD 300 (4 mm) with Peristaltic Pump......................................................... 064905
CRD 300 (2 mm) with Peristaltic Pump......................................................... 064906
Santoprene Tubing for Peristaltic Pump (30 cm).......................................... 069414
Peristaltic Pump / CRD 300 Ship Kit............................................................. 064911
For configuring two CRD 300s with one peristaltic pump
CRD 300 (4 mm) with VP Vacuum Pump....................................................... 068475
CRD 300 (2 mm) with VP Vacuum Pump....................................................... 068474
www.dionex.com 293
OnGuard II Cartridges OnGuard II Ag
The OnGuard II Cartridges remove matrix interferences such as The OnGuard II Ag contains a silver-form, high-capacity,
phenolics, metals, cations, anions, or hydrophobic substances sulfonated, cation-exchange resin similar to the OnGuard II H
encountered in many ion chromatography applications. Car- packing. These cartridges remove chloride, bromide, and iodide
tridges are available in 1 cc and 2.5 cc high-capacity formats. from sample matrices. An OnGuard II H cartridge should be
used after the OnGuard II Ag cartridge to remove dissolved Ag.
Related Literature Key Specifications

For detailed specifications and applications, see the following Functionality: cation-exchange silver form
Capacity (µeq/cartridge): 2.0–2.2 (1 cc); 5.0–5.5 (2.5 cc)
Product Data Sheets Solvents: 0–100% HPLC
OnGuard II Sample Pretreatment Cartridges and Workstation pH Stability: 0–14

Mode: removal of chloride, bromide, iodide by precipitation
Application Notes
AN 101: Trace Level Determination of Bromate in Ozonated Ordering Information

Drinking Water Using Ion Chromatography
AN 136: Determination of Inorganic Oxyhalide Disinfection Accessories

Byproduct Anions and Bromide in Drinking Water Using Ion OnGuard II Ag Cartridges, 1 cc, Pkg. of 48................................................... 057089
Chromatography with the Addition of a Postcolumn Reagent for
Trace Bromate Analysis OnGuard II Ag Cartridges, 2.5 cc, Pkg. of 48................................................ 057090
AN 37: The Determination of Iodide in Milk Products

OnGuard II Ag/H
Application Updates
The OnGuard II Ag/H is a layered cartridge that contains both
AU 140: The Determination of Iodide in Urine OnGuard II Ag and OnGuard II H resins.
• Easily removes chloride, bromide, and iodide from concen-

OnGuard II A trated matrices such as brines
The OnGuard II A is used in the removal of anionic contami- • Traps soluble silver and other cations
nants from sample matrices and for the neutralization of highly
• Removes high levels of alkaline earth and transition metals
acidic samples. These cartridges contain styrene-based, anion-
exchange resin in the bicarbonate form. • Neutralizes caustic samples
• Removes carbonate
Key Specifications
This two-layer cartridge replaces two cartridges in series, and
Functionality: anion-exchange bicarbonate form provides greater silver capacity.
Capacity (µeq/cartridge): 0.7 (1 cc cartridge); 1.75 (2.5 cc
cartridge) Ordering Information
Solvents: 0–100% HPLC
pH stability: 0–14 Accessories
OnGuard II Ag-H Cartridge, 2.5 cc, Pkg of 48............................................... 057410
Mode: removal of anions; pH adjustments of acidic samples
Accessories
OnGuard II A Cartridges, 1 cc, Pkg. of 48..................................................... 057091
OnGuard II A Cartridges, 2.5 cc, Pkg. of 48.................................................. 057092
294 www.dionex.com
OnGuard II Cartridges
OnGuard II Ba OnGuard II H
The OnGuard II Ba resin is a styrene-based, sulfonic acid resin The OnGuard II H removes high levels of alkaline earth and
in the barium form, designed for the removal of high concentra- transition metals from sample matrices and neutralizes highly
tions of sulfate from sample matrices. For reproducible, quan- alkaline samples such as sodium hydroxide or sodium carbon-
titative determinations in low-ionic-strength samples, activate ate. Carbonate can then be removed by sparging the sample.
these cartridges by adding NaCl or other sodium salt.
These cartridges contain 16% cross-linked, styrene-based,
Samples treated with NaCl should be passed through an On- sulfonic acid resin in the hydrogen form. This resin is designed
Guard II Ag cartridge to remove the added chloride, then passed to have very high selectivity for multivalent cations such as
through an OnGuard II H cartridge or MetPac™ CC-1 chelating calcium and transition metals.
column to remove residual silver counter ions.
Key Specifications
Key Specifications
Functionality: cation-exchange hydronium form
Functionality: cation-exchange barium form
pH Stability: 0–14
Mode: removal of alkaline earth and transition metals; pH ad-
Mode: removal of sulfate by precipitation justment of basic samples
Ordering Information Ordering Information
Accessories Accessories
OnGuard II Ba Cartridges, 1 cc, Pkg. of 48.................................................... 057093 OnGuard II H Cartridges, 1 cc, Pkg. of 48..................................................... 057085
OnGuard II Ba Cartridges, 2.5 cc, Pkg. of 48................................................ 057094 OnGuard II H Cartridges, 2.5 cc, Pkg. of 48.................................................. 057086
OnGuard II Ba/Ag/H OnGuard II M
The OnGuard II Ba/Ag/H is a layered cartridge containing On- The OnGuard II M is used for the removal of transition metals
Guard II Ba, Ag, and H styrene-based, sulfonic acid resins. and for matrix elimination of alkali and alkaline earth metals.
These cartridges contain an iminodiacetate resin in the ammo-
• The Ba resin removes high concentrations of sulfate from nium form, ready to use with no lengthy preparation required.
sample matrices.
• The Ag form easily removes chloride, bromide, and iodide Key Specifications
from concentrated matrices.
Functionality: iminodiacetate ammonium form
• The H form is highly selective for polyvalent cations such
as calcium and transition metals. Capacity (µeq/cartridge): 0.4 (1 cc); 1.0 (2.5 cc)
This cartridge is ideal for the removal of high levels of alkaline
earth and transition metals from sample matrices, neutralization pH Stability: 0–14 (resin shrinks in acid form)
of caustic samples, and removal of carbonate. This three-layer
cartridge can be used in place of three single cartridges in series Mode: concentration of transition metals by chelation (2.5 cc
and has the added advantage of higher silver capacity. format); removal of transition metals (1 cc format)
Accessories
Accessories
OnGuard II M Cartridges, 1 cc, Pkg. of 48.................................................... 057137
OnGuard II Ba/Ag/H Cartridges, 2.5-cc, Pkg. of 48...................................... 063955
OnGuard II M Cartridges, 2.5 cc, Pkg. of 48................................................. 057095
www.dionex.com 295
OnGuard II Na OnGuard II RP
The OnGuard II Na removes high levels of alkaline earth and The OnGuard II RP cartridge is recommended for removing
transition metals from sample matrices without acidifying the hydrophobic substances such as aromatic dyes, some aromatic
sample. This ensures good recovery of acid-labile analytes such carboxylic acids, hydrocarbons, and surfactants from sample
as nitrite. These cartridges contain 16% cross-linked, styrene- matrices. These cartridges contain a macroporous divinylben-
based, sulfonic acid resin in the sodium form. This resin has zene resin that has a very high selectivity for hydrophobic sub-
very high selectivity for multivalent cations such as calcium, stances, especially unsaturated or aromatic organic substances.
magnesium and transition metals.
Contains 0.3 g resin/1 cc cartridge, 0.75 g resin 2.5 cc cartridge.
Key Specifications
Key Specifications
Functionality: cation-exchange sodium form
Functionality: polydivinylbenzene
Capacity (µeq/cartridge) : 2.0–2.2 (1 cc); 5.0–5.5 (2.5 cc)
Capacity (µeq/cartridge) : 0.3 g resin (1 cc); 0.75 g res-
in (2.5 cc)
Mode: removal of alkaline earth and transition metals without
acidifying the sample
Mode: removal of surfactants, high-molecular-weight carboxy-
Ordering Information lic acids, aromatic dyes by adsorption
Accessories Ordering Information
OnGuard II Na Cartridges, 1 cc, Pkg. of 48................................................... 062948

Accessories
OnGuard II Na Cartridges, 2.5 cc, Pkg. of 48................................................ 062962
OnGuard II RP Cartridges, 1 cc, Pkg. of 48.................................................... 057083
OnGuard II RP Cartridges, 2.5 cc, Pkg. of 48................................................ 057084
OnGuard II P
The OnGuard II P is recommended for removing the phenolic OnGuard Accessories

fraction of humic acids, tannic acids, lignins, anthocyanins, and
azodyes from samples prior to analysis by ion exchange. The OnGuard Sample Prep Station enables simultaneous pre-
treatment of multiple samples with OnGuard sample pretreat-
These cartridges contain polyvinylpyrrolidone (PVP) polymer ment cartridges. When used with Dionex 0.5 mL PolyVials and
with very high selectivity for phenolics, azo-containing com- a vacuum source, the OnGuard Sample Prep Station supports
pounds, aromatic carboxylic acids, aromatic aldehydes, and semi-automatic pretreatment of up to 12 samples. Samples may
iodine as the triiodide complex. also be treated manually using standard Luer tip syringes.
The OnGuard Sample Prep Station has individual stopcock

Key Specifications
valves on each sample tube to allow control of individual flow
Functionality: polyvinylpyrrolidone rates. The station will also hold six 10 mL volumetric flasks.
Capacity (µeq/cartridge) : 6.0 (1 cc); 2.5 cc format not avail.

pH Stability: 1–10 Accessories
Mode: phenol, azo dye, humic acid removal by complexation OnGuard Sample Prep Workstation.............................................................. 039599
OnGuard Needle, 18 Gauge, 1.25/Luer........................................................ 039996
Ordering Information OnGuard Valve, Stopcock Luer...................................................................... 040896
Accessories
OnGuard II P Cartridges, 1 cc, Pkg. of 48...................................................... 057087
296 www.dionex.com
Standards, Reagents, and Eluent Concentrates
Standards, Reagents, and Eluent MS Standard Concentrates
Concentrates
To simplify your workflow, Dionex has developed all the re-
agents and standards you need, including anion and cation stan-
MS Standard Concentrates
dards, eluent concentrates, and AutoRegen concentrates. Dionex
offers high-purity chemicals and standards for transition metal Kit of Standards for OQ/PQ Measurements (FM104284)............................ 061496
analysis, including ultrapure chelation chromatography reagents Calibrant for MSQ ELMO and Plus, 250 mL (60111-01001)......................... 062917
for trace and ultratrace transition and lanthanide metal analysis. Mass Calibration Solution Mixture (FM104285).......................................... 060758
Perchlorate O-18 Internal Standard, 1 mg/L, 10 mL.................................... 062923
Related Literature
OQ/PQ Standards
Product Data Sheets

OQ/PQ Standards
IC Standards and Reagents Data Sheet Custom Caffeine Standards Kit, 10 mL Ampoules....................................... 060253
Custom Nitrate Standards Kit, 10 mL Ampoules......................................... 060254
Ion Standard Concentrates Threonine Standards Kit, set of 6, 1 mL ea.................................................. 063542
Ordering Information Anion Eluent Concentrates
Ion Standard Concentrates Ordering Information

5-Anion Standard, 100 mL............................................................................ 037157
Contains: F, 20 mg/L; Cl, 30 mg/L; NO2, 100 mg/L; PO4, 150 mg/L; SO4, 150 mg/L Anion Eluent Concentrates
7-Anion Standard, 50 mL.............................................................................. 056933 AS23 Eluent Concentrate (100x), 250 mL..................................................... 064161
Contains: F, 20 mg/L; Cl, 30 mg/L; NO2, 100 mg/L; Br, 100 mg/L; NO3, 100 mg/L;
PO4, 150 mg/L; SO4, 150 mg/L AS22 Eluent Concentrate (100x), 250 mL..................................................... 063965
AS14A Eluent concentrate (100x), 250 mL................................................... 056937
7-Anion Standard II, 100 mL......................................................................... 057590
Contains: F, 20 mg/L; Cl, 30 mg/L; NO2, 100 mg/L; Br, 100 mg/L; NO3, 100 mg/L; AS14A Eluent Concentrate (20x), 100 mL.................................................... 057560
PO4, 150 mg/L; SO4, 150 mg/L AS14A Eluent Concentrate (20x), 100 mL, Pkg of 4..................................... 057557
Fluoride Standard (1000 mg/L), 100 mL........................................................ 037158 AS14 Eluent concentrate (100x), 250 mL..................................................... 053560
Chloride Standard (1000 mg/L), 100 mL....................................................... 037159 AS4A Eluent Concentrate (100x), 500 mL.................................................... 039513
Sulfate Standard (1000 mg/L), 100 mL......................................................... 037160 AS4 Eluent Concentrate (100x), 500 mL...................................................... 037161
6-Cation Standard-I, 50 mL........................................................................... 040187 Carbonate Concentrate, 0.5M, 500 mL........................................................ 037162
Contains: Li, 50 mg/L; Na, 200 mg/L; NH4, 400 mg/L; K, 200 mg/L; Bicarbonate Concentrate, 0.5M, 500 mL...................................................... 037163
Mg, 200 mg/L; Ca, 1000 mg/L
6-Cation Standard-II, 50 mL.......................................................................... 046070
Contains: Li, 50 mg/L; Na, 200 mg/L; NH4, 250 mg/L; K, 500 mg/L;
Mg, 250 mg/L; Ca, 500 mg/L
www.dionex.com 297
Cation Eluent Concentrates and Transition Metal Analysis Reagents
Reagents
Transition Metal Analysis Reagents
Cation Eluent Concentrates and Reagents MetPac Oxalic Acid Eluent Concentrate (10x), 500 mL................................ 046091
Methanesulfonic Acid, 500 mL..................................................................... 033478 MetPac PDCA Eluent Concentrate (5x), 1000 mL......................................... 046088
For IonPac CS12, CS12A, CS14, CS15, CS17, and CS18 cation exchange column PDCA (Pyridine-2,6-dicarboxylic Acid), 20g.................................................. 039671
eluents. MetPac PAR Post Column Diluent, 1000 mL................................................ 046094
CS12A Eluent Concentrate (20x), 100 mL..................................................... 057562 Ready-to-use diluent for 4-(2-Pyridylazo) Resorcinol
CS12A Eluent Concentrate (20x), 100 mL, Pkg of 4..................................... 057558 PAR (4-(2-Pyridylazo)Resorcinol Monosodium Salt),5g................................ 039672
DAP-HCl (DL-2,3-Diaminopropionic Acid-HCl), 5g....................................... 039670 Required for transition metal and lanthanide postcolumn derivitization using
For IonPac CS3, CS10, and CS11 cation exchange column eluents the IonPac CS5 or CS5A columns
Displacement Chemical Regen. Chelation Chromatography Reagents

Reagents
Chelation Chromatography Reagents
Displacement Chemical Regeneration Reagents 2.0 M Nitric Acid, 1 L.................................................................................... 033442
Anion Regenerant Concentrate (2.0N H2SO4), 75 mL................................... 057559 2.0 M Nitric Acid, 1 L, Pkg of 6..................................................................... 033443
Anion Regenerant Concentrate (2.0N H2SO4), 75 mL, Pkg of 4................... 057555 2.0 M Ammonium Acetate, 1 L..................................................................... 033440
Cation Regenerant Solution (2.06 M TBAOH), 100 mL................................ 057561 2.0 M Ammonium Acetate, 1 L, Pkg of 6...................................................... 033441
Cation Regenerant Solution (2.06 M TBAOH), 100 mL, Pkg of 4................. 057556 0.1 M Ammonium Nitrate, 1 L...................................................................... 033444
0.1 M Ammonium Nitrate, 1 L, Pkg of 6....................................................... 033445
MMS Regenerant Concentrates

Carbohydrate Standards
MMS Regenerant Concentrates
Carbohydrate Standards
Anion Regenerant Concentrate (10x), 50 mL................................................ 039601
Anion Regenerant Concentrate (20x), 200 mL.............................................. 039203 MonoStandard, Mixture of Six, 100 nmol each........................................... 043162
Anion Regenerant Concentrate (20x), 200 mL, Pkg of 4.............................. 037164 OligoStandard, Sialylated N-Linked Alditols,25 nmol................................. 043064
Cation Regenerant Solution, 500 mL............................................................ 039602
AAA-Direct Reagents
Ion Pairing Reagents
AAA-Direct Reagents
Ion Pairing Reagents Sodium Acetate Salt (Pre-weighed Reagent), 2-pack.................................. 059326
Tetrabutylammonium hydroxide (TBAOH), 500 mL...................................... 035360 Histidine Standard, AAA-direct system Installation.................................... 059540
Tetrapropylammonium hydroxide (TPAOH), 500 mL..................................... 035363
Hexanesulfonic acid (HSA), 500 mL.............................................................. 035361
Octanesulfonic Acid (OSA), 500 mL.............................................................. 035362
298 www.dionex.com
ICS-900 Solutions Kits
ICS-900 Solutions Kits AS12A 4 mm Bundled Package
These bundled packages include: analytical and guard col- This package includes:
umns, appropriate MMS 300 suppressor, application-specific
DCR bottle, eluent, and regenerant concentrates. These kits are • IonPac AS12A 4 mm, P/N 046034
appropriate for the ICS-900, ICS-90, ICS-90A, and previous • IonPac AG12A 4 mm, P/N 046035
Displacement Chemical Regeneration systems. Bundles are also
available without the eluent and regenerant concentrates. These • AMMS 300 4 mm, P/N 064558
are recommended for customers outside the United States.
• Anion Regenerant Concentrate, 4 pack, P/N 057555
• ICS-900 DCR Anion Regenerant 2 L Bottle, P/N 057712
AS4A 4 mm Bundled Package • Sodium Carbonate Concentrate (0.5 Molar), 500 mL, P/N
037162
This package includes:
• Sodium Bicarbonate Concentrate (0.5 Molar), 500 mL, P/N
• IonPac AS4A-SC 4 mm, P/N 043174 037163
• IonPac AG4A-SC 4 mm, P/N 043175
• AMMS 300 4 mm, P/N 064558
• Anion Regenerant Concentrate, 4 pack, P/N 057555 ICS-900 - AS12A 4 mm - Bundled Package
• ICS-900 DCR Anion Regenerant 2 L Bottle, P/N 057712 ICS-900, AS12A 4 mm, Consumables Bundled Package............................. 060135
• AS4A-SC Eluent Concentrate, P/N 039513 ICS-900, AS12A 4 mm Bundle, No Chemicals or Bottle.............................. 069235
Ordering Information AS14 4 mm Bundled Package

ICS-900 - AS4A 4 mm - Bundled Package
ICS-900, AS4A 4 mm, Consumables Bundled Package............................... 060133 • IonPac AS14 4 mm, P/N 046124
ICS-900, AS4A 4 mm Bundle, No Chemicals or Bottle................................ 069233 • IonPac AG14 4 mm, P/N 046134
• AMMS 300 4 mm, P/N 064558
AS9-HC 4 mm Bundled Package
• ICS-900 DCR Anion regenerant 2 L Bottle, P/N 057712
• IonPac AS9-HC 4 mm, P/N 051786 • AS14 Eluent Concentrate, P/N 053560
• IonPac AG9-HC 4 mm, P/N 051791
• AMMS 300 4 mm, P/N 064558
• Anion Regenerant Concentrate, 4 pack, P/N 057555 ICS-900 - AS14 4 mm - Bundled Package
• ICS-900 DCR Anion Regenerant 2 L Bottle, P/N 057712 ICS-900, AS14 4 mm, Consumables Bundled Package................................ 060136
• Sodium Carbonate Concentrate (0.5 Molar), 500 mL, P/N ICS-900, AS14 4 mm Bundle, No Chemicals or Bottle................................ 069236
037162
• Sodium Bicarbonate Concentrate (0.5 Molar), 500 mL, P/N
037163
ICS-900 - AS9-HC 4 mm - Bundled Package

ICS-900, AS9-HC 4 mm, Consumables Bundled Package............................ 060134
ICS-900, AS9-HC 4 mm Bundle, No Chemicals or Bottle............................ 069234
www.dionex.com 299
AS14A 4 mm Bundled Package Ordering Information

ICS-900 - AS22 4 mm - Bundled Package
• IonPac AS14A 4 mm, P/N 056904 ICS-900, AS22 4 mm, Consumable Bundled Package................................. 067078
ICS-900, AS22 4 mm Bundle, No Chemicals or Bottle................................ 069238
• IonPac AG14A 4 mm, P/N 056897
• AMMS 300 4 mm, P/N 064558
AS23 4 mm Bundled Package
• AS14A Eluent Concentrate, P/N 056937 • IonPac AS23 4 mm, P/N 064149
• IonPac AG23 4 mm, P/N 064147
• AMMS 300 4 mm, P/N 064558
ICS-900 - AS14A 4 mm Bundled Package • AMMS III Regenerant Concentrate, 4 pack, P/N 057555
ICS-900, AS14A 4 mm, Consumables Bundled Package............................. 060137 • ICS-900 DCR Anion Regenerant 2 L Bottle, P/N 057712
ICS-900, AS14A 4 mm Bundle, No Chemicals or Bottle.............................. 069237 • AS23 Eluent Concentrate, P/N 064161
AS14A 3 mm Bundled Package Ordering Information
• This package includes:

ICS-900 - AS23 4 mm - Bundled Package
• IonPac AS14A 3 mm, P/N 056901
ICS-900, AS23 4 mm, Consumable Bundled Package................................. 067079
• IonPac AG14A 3 mm, P/N 056899 ICS-900, AS23 4 mm Bundle, No Chemicals or Bottle................................ 069239
• AMMS 300 2 mm, P/N 064559
• Anion Regenerant Concentrate, 4 pack, P/N 057555 CS12A 4 mm Bundled Package
• ICS-900 DCR Anion Regenerant 2 L Bottle, P/N 057712 This package includes:
• AS14A Eluent Concentrate, P/N 056937 • IonPac CS12A 4 mm, P/N 046073
• IonPac CG12A 4 mm, P/N 046074
• CMMS 300 4 mm, P/N 064560
ICS-900 - AS14A 3 mm - Bundled Package • Cation Regenerant Concentrate, 4 pack, P/N 057556
ICS-900, AS14A 3 mm, Consumables Bundled Package............................. 060138 • ICS-900 DCR Cation Regenerant 2 L Bottle, P/N 057713
ICS-900, AS14A 3 mm Bundle, No Chemicals or Bottle.............................. 069244
• CS12A Eluent Concentrate, P/N 057562
AS22 4 mm Bundled Package Ordering Information

ICS-900 - CS12A 4 mm - Bundled Package
• IonPac AS22 4 mm, P/N 064141
ICS-900, CS12A 4 mm, Consumables Bundled Package............................. 060132
• IonPac AG22 4 mm, P/N 064139 ICS-900, CS12A 4 mm Bundle, No Chemicals or Bottle.............................. 069241
• AMMS 300 4 mm, P/N 064558
• AS22 Eluent Concentrate, P/N 067078
300 www.dionex.com
ICS-900 Solutions Kits
CS12A 3 mm Bundled Package CS16 3 mm Bundled Package
This package includes: This package includes:
• IonPac CS12A 3 mm, P/N 057185 • IonPac CS16 3 mm, P/N 059596
• IonPac CG12A 3 mm, P/N 057184 • IonPac CG16 3 mm, P/N 059595
• CMMS 300 2 mm, P/N 064561 • CMMS 300 2 mm, P/N 064561
• Cation Regenerant Concentrate, 4 pack, P/N 057556 • Cation Regenerant Concentrate, 4 pack, P/N 057556
• ICS-900 DCR Cation Regenerant 2 L Bottle, P/N 057713 • ICS-900 DCR Cation Regenerant 2 L Bottle, P/N 057713
• CS12A Eluent Concentrate, P/N 057562 • Methanesulfonic Acid, 500 mL, P/N 033478
Ordering Information Ordering Information
ICS-900 - CS12A 3 mm - Bundled Package ICS-900 - CS16 3 mm - Bundled Package
ICS-900, CS12A 3 mm, Consumables Bundled Package............................. 060131 ICS-900, CS16 3 mm, Consumables Bundled Package................................ 060130
ICS-900, CS12A 3 mm Bundle, No Chemicals or Bottle.............................. 069240 ICS-900, CS16 3 mm Bundle, No Chemicals or Bottle................................. 069242
CS16 5 mm Bundled Package

• IonPac CS16 5 mm, P/N 057573

• IonPac CG16 5 mm, P/N 057574
• CMMS 300 4 mm, P/N 064560
• Cation Regenerant Concentrate, 4 pack, P/N 057556
• ICS-900 DCR Cation Regenerant 2 L Bottle, P/N 057713
• Methanesulfonic Acid, 500 mL, P/N 033478
ICS-900 - CS16 5 mm - Bundled Package

ICS-900, CS16 5 mm, Consumables Bundled Package................................ 060129
ICS-900, CS16 5 mm Bundle, No Chemicals or Bottle................................. 069243
www.dionex.com 301
LCi Solutions Kits General LCi Solutions Kits
LCi Solutions Kits provide preconfigured capillary kits and

comprehensive quick installation guides for fast and convenient
implementation. See “LCi Solutions” in the LC Instruments sec- The LCi Solution kits for 2-D LC, automated application
tion for discussion of Dionex LCi Solutions. switching, automated method scouting, on-line SPE, and paral-
lel LC contain capillaries which are preconfigured for system
• 2-D LC analysis
configurations that include the TCC-3x00. The kits are not suit-
• Automated application switching able for system configurations that include the TCC-3000SD or
TCC-3000RS.
• Automated method scouting
• On-line SPE analysis
2-D LC Kits
• Parallel analysis 2-D LC Tubing Kit for Analytical Dual-Ternary UltiMate 3000 Systems.6037.0008
• Tandem analysis
Automated Application Switching Kits
• Rapid Separation LC for quaternary and binary analytical
systems Application Switching Tubing Kit for Analytical Dual-Ternary UltiMate
3000 Systems........................................................................................... 6037.0009
• Proteomics preconcentration, parallel LC, and MDLC Application Switching Tubing Kit for Micro Dual-Ternary UltiMate
3000 Systems........................................................................................... 6037.0010
Learn more about LCi Solutions on www.dionex.com under LC
and LC/MS. Contact your local Dionex representative for an Automated Method Scouting Kits
UltiMate 3000 LCi system configuration customized for your
applications. Automated Method Scouting Kit for Systems with Quaternary
Analytical Pump (e.g., LPG-3400A).......................................................... 6722.0101
Automated Method Scouting Kit for Systems with Quaternary Micro
Pump (e.g., LPG-3400M)........................................................................... 6722.0100
On-Line SPE Kits

On-line Sample Preparation Tubing Kit for Analytical Dual-Ternary
UltiMate 3000 Systems........................................................................... 6037.0006

On-line Sample Preparation Tubing Kit for Micro Dual-Ternary
UltiMate 3000 Systems........................................................................... 6037.0007
Parallel LC Kits
Parallel Operation Tubing Kit for Analytical Dual-Ternary UltiMate
3000 Systems........................................................................................... 6037.0004
Parallel Operation Tubing Kit for Micro Dual-Ternary UltiMate 3000
Systems.................................................................................................... 6037.0005
Tandem LC Kits
Tandem Operation Tubing Kit for Analytical Dual-Ternary UltiMate
3000 Systems........................................................................................... 6037.0001
Tandem Operation Tubing Kit for Micro Dual-Ternary UltiMate 3000
Systems.................................................................................................... 6037.0002
RSLC Conversion Kits

RSLC Conversion Kits for Quaternary Analytical UltiMate 3000
Systems.................................................................................................... 6037.0014
RSLC Conversion Kits for Binary Analytical UltiMate 3000 Systems.... 6037.0015
302 www.dionex.com
LCi Solutions Kits
LCi Solutions Kits for Proteomics Off-Line 2-D LC Kits
Automated Off-Line 2-D LC Solution, WPS-3000PL................................ 6820.0050
Ordering Information Automated Off-Line 2-D LC Solution, Biocompatible, WPS-3000PL...... 6821.0050
Off-Line 2-D LC Kit for Peptides, Polysulfoethyl ASP 300 µm i.d./
PepSwift 200 µm i.d................................................................................. 6720.0100
Preconcentration Kits
Off-Line 2-D LC Kit for Peptides, Polysulfoethyl ASP 300 µm i.d./
Preconcentration Kit, Nano...................................................................... 6720.0047 PepSwift 200 µm i.d., Biocompatible...................................................... 6721.0100
Preconcentration Kit, Capillary................................................................ 6720.0048 Off-Line 2-D LC Kit for Peptides, Polysulfoethyl ASP 1.0 mm i.d./
Preconcentration Kit, Monolithic............................................................. 6720.0049 PepMap 100 C18 75 µm i.d...................................................................... 6720.0101
Preconcentration Kit, Micro..................................................................... 6720.0050 Off-Line 2-D LC Kit for Peptides, Polysulfoethyl ASP 1.0 mm i.d./
PepMap 100 C18 75 µm i.d., Biocompatible........................................... 6721.0101
Preconcentration Kit, Nano, Biocompatible............................................ 6721.0047
Off-Line 2-D LC Kit for Proteins, ProSwift WAX 1.0 mm i.d./
Preconcentration Kit, Capillary, Biocompatible....................................... 6721.0048
PepSwift 200 µm i.d................................................................................. 6720.0102
Preconcentration Kit, Monolithic, Biocompatible................................... 6721.0049
Preconcentration Kit, Micro, Biocompatible........................................... 6721.0050 PepSwift 200 µm i.d., Biocompatible...................................................... 6721.0102
Proteomics Parallel LC Kits PepSwift 500 µm i.d................................................................................. 6720.0103
Parallel LC Kit, Nano/Nano...................................................................... 6720.0054 Off-Line 2-D LC Kit for Proteins, ProSwift WAX 1.0 mm i.d./
PepSwift 500 µm i.d., Biocompatible...................................................... 6721.0103
Parallel LC Kit, Capillary/Capillary........................................................... 6720.0055
Parallel LC Kit, Monolithic/Monolithic.................................................... 6720.0056
Parallel LC Kit, Nano/Nano, Biocompatible............................................ 6721.0054
Comprehensive 2-D LC Kits
Parallel LC Kit, Capillary/Capillary, Biocompatible................................. 6721.0055 Comprehensive 2-D LC Kit, Nano............................................................ 6720.0053
Parallel LC Kit, Monolithic/Monolithic, Biocompatible.......................... 6721.0056 Comprehensive 2-D LC Kit, Nano, Biocompatible................................... 6721.0053
Salt Plug Application Kits

2-D LC Salt Plug Kit, Nano....................................................................... 6720.0051
2-D LC Salt Plug Kit, Capillary.................................................................. 6720.0052
2-D LC Salt Plug Kit, Monolithic.............................................................. 6720.0067
2-D LC Salt Plug Kit, Nano, Biocompatible............................................. 6721.0051
2-D LC Salt Plug Kit, Capillary, Biocompatible........................................ 6721.0052
2-D LC Salt Plug Kit, Monolithic, Biocompatible..................................... 6721.0067
www.dionex.com 303
304 www.dionex.com
Indices & Appendices
Column Selection Guide 307 Technical Notes................................................... 326

IC/HPLC Technical Notes................................................. 326
Silica Columns..................................................... 307 ASE Technical Notes........................................................ 327
Mass Spectrometry Application Briefs and Technical
Polymer Columns................................................ 309 Notes................................................................................... 327
Automation Product Technical Notes........................... 327
Books.................................................................... 327
Column Specifications 311
IC Anion Columns............................................... 311

Service & Technical Support 328
IC Cation Columns.............................................. 313
Comprehensive Service Plans........................... 328
Ion-Exclusion Columns....................................... 314 Superior Service................................................................ 328
Acclaim General and Specialty Columns......... 314 Validation Services.............................................. 328

Automating the Validation Process............................... 328
Bio Columns........................................................ 314
Peak Performance Kits........................................ 328
Protein................................................................................. 314
Carbohydrate..................................................................... 316
DNA...................................................................................... 316 Contact Information............................................ 328
Dionex Literature 317 Training 329
General Interest................................................... 317 Course Offerings................................................. 329

Applications Booklets/CD-ROMs/s................................ 317 Instrumentation................................................................. 329
Software.............................................................................. 329

Product Brochures.............................................. 317
IC/HPLC Products.............................................................. 317 Training Options.................................................. 329
Accelerated Solvent Extraction (ASE).......................... 317 Specialized......................................................................... 329
Customized Training.......................................................... 329
Product Data Sheets........................................... 317 On-Site Training................................................................. 329
IC/HPLC Product Data Sheets........................................ 317 Web-Based Training......................................................... 329
Accelerated Solvent Extraction (ASE).......................... 318
Automation & Software................................................... 318
Dionex Columns................................................................. 318
Index of Part Numbers 330
Suppressors....................................................................... 319
Reagents, Standards, and Accessories....................... 319
Application Notes................................................ 319 Ordering Information 337

IC/HPLC Application Notes............................................. 319
ASE Application Notes..................................................... 323 Dionex Locations ............................................... 337
Nano and Capillary LC Application Notes.................... 324 Corporate Headquarters.................................................. 337
Worldwide Offices and Subsidiaries............................. 337
Application Updates........................................... 325
www.dionex.com 305

306
www.dionex.com
Column Selection Guide
Silica Columns Resersed-Phase LC Specialty LC
Acclaim Polar Advantage II (PA2)

Acclaim Polar Advantage (PA)
Acclaim Mixed-Mode HILIC

Acclaim Mixed-Mode WAX
Acclaim Mixed-Mode WCX
Acclaim Explosives E1
Acclaim Explosives E2
Example Applications
Acclaim Organic Acid

Acclaim Surfactant
Acclaim 120 C18
Acclaim 300 C18

Acclaim 120 C8
High hydrophobicity √ √ √ √ √ √ √ √ fat-soluble vitamins, dyes
Neutral Intermediate hydrophobicity √ √ √ √ √ √ √ √ steroids, phthalates, phenolics
Low hydrophobicity √ √ √ √ √ acetaminophen, urea, polyethylene glycols
High hydrophobicity √ √ √ √ √ √ √ √ NSAIDs, phospholipids
Anions Intermediate hydrophobicity √ √ √ √ √ √ √ aspirin, PFOA, preservatives
General Applications
Low hydrophobicity √ √ √ √ organic acids, food dyes

High hydrophobicity √ √ √ √ √ √ √ √ antidepressants
Cations Intermediate hydrophobicity √ √ √ √ √ √ √ beta blockers, benzidines, alkaloids
Low hydrophobicity √ √ antacids, pseudoephedrine, amino sugars
Amphoteric/ Small molecules √ √ √ √ amino acids, aspartame
Zwitterions Peptides √ √
Neutrals and acids √ √ √ √ artificial sweeteners

Neutrals and bases √ √ √ √ cough syrup
Mixtures
Acids and bases √ √ drug active ingredient with counterion
Neutrals, acids, and bases √ √ combination pain relievers
Anionic √ √ SDS, LAS, laureth sulfates
Cationic √ benzylalkonium, quaternary amines
Non-ionic √ √ Triton, Brij
Surfactants
Amphoteric √ cocoamidopropyl betaine
Hydrotropes √ xylenesulfonates
Specific Applications
Surfactant blends √ consumer products

Organic Hydrophobic √ √ aromatic acids, fatty acids
Acids Hydrophilic √ √ small organic acids
Phenoxy √ √ √ √ √
Pesticides/ Triazine √ √ √ √ √
Herbicides Glyphosate √
Carbamates (EPA 531) √ √ √
Explosives (EPA 8330) √ √
Phenols √
Other
Carbonyl compounds (CARB 1004) √
Vitamin analysis √ √
See also the Acclaim Library of related applications on www.dionex.com under Literature, Application Notes (right side of the page).
www.dionex.com 307
Polymer Columns
IonPac AS9/HC/SC
IonPac AS11(-HC)
OmniPac PAX100
OmniPac PAX500
IonPac AS4A/SC
IonPac AS14/A
IonPac AS17-C
IonPac AS12A
FastAnion IIIA
IonPac AS23
IonPac AS22
IonPac AS24
IonPac AS21
IonPac AS20
IonPac AS19
IonPac AS18
IonPac AS16
IonPac AS15
IonPac AS10
IonPac CS18
IonPac AS7
IonPac AS5
IonPac
Inorganic Anions √ √ √ √ √ √ √ √ √ √ √ √ √ √
Oxyhalides √ √ √ √ √
Bromate √ √ √ √
Perchlorate √ √ √ √
ANIONS
Organic Acids √ √ √ √
Phosphoric/Citric Acids √
Poly/High-Valence Anions √ √ √ √ √
Hydrophobic Anions √ √ √
Hydrophobic/Halogenated Anions √ √ √
Anionic Neutral Molecules √ √ √ √ √
Inorganic Cations √
Sodium/Ammonium
Amines/Polyvalent Amines √
Aliphatic/Aromatic Amines
CATIONS
Alkanol/Ethhanolamines √
Biogenic Amines √
Transition/Lanthanide Metals
Hydrophobic Cations √
Cationic Neutral Molecules

Amino Acids
Phosphorylated Amino Acids
Amino Sugars
Oligosccharides
BIO-MOLECULES
Mono-/Di-Saccharides
Glycoproteins
Alditols/Aldoses mono/di Saccharides
ds Nucleic Acids
Single-Stranded Oligonucleotides
Peptides
Proteins
Metal-binding Proteins
Monoclonal antibodies
Aliphatic Organic Acids
Alcohols
ORGANIC MOLECULES
Borate
Large Molecules, Anions
Small Molecules
Small Molecules/LC-MS
Polar/Non-Polar Small Molecules
Hydrophobic/Aliphatic Organic Acids
Surfactant Formulations
Explosives/EPA 8330
Anion Exchange / Carbonate √ √ √ √ √ √
Anion Exchange / Hydroxide √ √ √ √ √ √ √ √ √ √ √ √ √
Cation Exchange √
MODE
Multi-Mode √ √
Affinity
Ion Exclusion
Reversed Phase
Anion Exchange/Other
308 www.dionex.com

√
√
√
√
√
√
√
IonPac CS17
√
√
√
IonPac CS16
√
√
√
√
IonPac CS15
√
√
√
√
IonPac CS14
√
√
√
√
IonPac CS12A
√
√
IonPac CS11
√
√
IonPac CS10
√
√
IonPac CS5A
√
√
OmniPac PCX100
√
√
OmniPac PCX500
√
√
√
√
AminoPac PA10
√
√
AminoPac PA1
√
√
√
√
CarboPac PA200
√
√
√
√
CarboPac PA100
√
√
√
√
CarboPac PA20
√
√
√
√
CarboPac PA10
√
√
√
√
√
CarboPac PA1
√
√
√
√
www.dionex.com
CarboPac MA1
√
√
√
√
DNAPac 200
√
√
√
√
√
DNAPac 100
√
√
√
√
ProPac WAX/SAX
√
√
√
√
ProPac WCX/SCX
√
√
√
√
√
ProPac IMAC
√
√
√
√
ProPac HIC
√
√
√
√
√
√
ProPac PA1
√
√
√
√
√
√
√
√
ProSwift
√
√
√
IonPac ICE-AS6
√
√
√
IonPac ICE-AS1
√
√ IonPac ICE-Borate
√
√
√
√
IonPac NS1
309
Column Selection Guide
310 www.dionex.com
Column Specifications
IC Anion Columns
Latex Diameter
Hydrophobicity
(per column)
Crosslinking
Crosslinking
Primary
Functional
Column Format Application
Substrate
Diameter
Capacity
Particle
Eluent
Group
Latex
IonPac 2 × 250 mm Hydroxide Recommended column for haloacetic 7 µm 55% - - 140 µeq Alkanol Ultralow
AS24 acids prior to MS or MS/MS quaternary
detection ammonium
IonPac 2 × 250 mm Carbonate Recommended column for inorganic 6 µm 55% - - 80 µeq Alkyl quaternary Ultralow
AS23 4 × 250 mm anions and oxyhalides. Trace bromate 320 µeq ammonium
in drinking water.
IonPac 2 × 250 mm Carbonate Recommended column for fast 6.5 µm 55% - - 52.5 µeq Alkyl quaternary Ultralow
AS22 4 × 250 mm analysis of common inorganic anions. 210 µeq ammonium
IonPac 2 × 250 mm Hydroxide Recommended column for trace 7.0 µm 55% - - 45 µeq Alkanol Ultralow
AS21 perchlorate prior to MS or MS/MS quaternary
detection ammonium
IonPac 2 × 250 mm Hydroxide Recommended column for trace 7.5 µm 55% - - 77.5 µeq Alkanol Ultralow
AS20 4 × 250 mm perchlorate prior to suppressed 310 µeq quaternary
conductivity detection. ammonium
IonPac 2 × 250 mm Hydroxide Recommended column for inorganic 7.5 µm 55% - - 60 µeq Alkanol Low
AS19 4 × 250 mm anions and oxyhalides. Trace bromate 350 µeq quaternary
in drinking water. ammonium
IonPac 2 × 250 mm Hydroxide Recommended column for the 7.5 µm 55% 65 8% 75 µeq Alkanol Low
AS18 4 × 250 mm analysis of common inorganic anions. nm 285 µeq quaternary
ammonium
IonPac 2 × 250 mm Hydroxide Trace anions in HPW matrices. 10.5 55% 75 6% 7.5 µeq Alkanol Low
AS17-C 4 × 250 mm Carboxylated resin, no sulfate blank. µm nm 30 µeq quaternary
Low capacity for fast analysis of ammonium
common inorganic anions using
gradient elution with the Eluent
Generator.
IonPac 2 × 250 mm Hydroxide High capacity for hydrophobic 9 µm 55% 80 1% 42.5 µeq Alkanol Ultralow

AS16 4 × 250 mm anions including iodide, thiocyanate, nm 170 µeq quaternary
thiosulfate, and perchlorate. ammonium
Polyvalent anions including:
polyphosphates and polycarboxylates
IonPac 2 × 250 mm Hydroxide High capacity for trace analysis of 9 µm 55% - - 56.25 µeq Alkanol Medium-
AS15 4 × 250 mm inorganic anions and low molecular 225 µeq quaternary High
weight organic acids in high purity ammonium
water matrices.
IonPac 3 × 150 mm Hydroxide Fast run, high capacity for trace 5 µm 55% - - 70 µeq Alkanol Medium-
AS15- analysis of inorganic anions and low quaternary High
5mm molecular weight organic acids in ammonium
high purity water matrices.
IonPac 3 × 150 mm Carbonate Recommended column for fast 5 µm 55% - - 40 ueq Alkyl quaternary Medium
AS14A- analysis of common inorganic anions. ammonium
5 µm
IonPac 4 × 250 mm Carbonate For analysis of common inorganic 7 µm 55% - - 120 µeq Alkyl quaternary Medium
AS14A anions. ammonium
IonPac 2 × 250 mm Carbonate Moderate capacity for fast analysis of 9 µm 55% - - 16 µeq Alkyl quaternary Medium-
AS14 4 × 250 mm common inorganic anions. 65 µeq ammonium High
www.dionex.com 311
Latex Diameter
Hydrophobicity
(per column)
Crosslinking
Crosslinking
Primary
Functional
Column Format Application
Substrate
Diameter
Capacity
Particle
Eluent
Group
Latex
IonPac 2 × 200 mm Carbonate Moderate capacity for analysis of 9 µm 55% 140 0.20% 13 µeq Alkyl quaternary Medium
AS12A 4 × 200 mm inorganic anions and oxyhalides. nm 52 µeq ammonium
Trace chloride and sulfate in high
carbonate matrices.
IonPac 2 × 250 mm Hydroxide High capacity for the determination of 9 µm 55% 70 6% 72.5 µeq Alkanol Medium-
AS11-HC 4 × 250 mm organic acids and inorganic anions in nm 290 µeq quaternary Low
uncharacterized samples. ammonium
IonPac 2 × 250 mm Hydroxide Low capacity for fast profiling of 13 µm 55% 85 6% 11 µeq Alkanol Very Low
AS11 4 × 250 mm organic acids and inorganic anions in nm 45 µeq quaternary
well-characterized samples. ammonium
IonPac 2 × 250 mm Hydroxide High capacity for the analysis of 8.5 µm 55% 65 5% 42.5 µeq Alkyl quaternary Low
AS10 4 × 250 mm inorganic anions and organic acids in nm 170 µeq ammonium
high nitrate samples.
IonPac 2 × 250 mm Carbonate High-capacity column for inorganic 9 µm 55% 90 18% 48 µeq Alkyl quaternary Medium-
AS9-HC 4 × 250 mm anions and oxyhalides. Trace bromate nm 190 µeq ammonium Low
in drinking water.
IonPac 4 × 250 mm Carbonate Low capacity for fast analysis of 13 µm 55% 110 20% 30-35 µeq Alkyl quaternary Medium-
AS9-SC inorganic anions and oxyhalides. nm ammonium Low
Specified column in US EPA Method
300.0 (B).
IonPac 2 × 250 mm Carbonate Low capacity for fast analysis of 13 µm 55% 160 0.50% 5 µeq Alkanol Medium-
AS4A-SC 4 × 250 mm common inorganic anions. Specified nm 20 µeq quaternary Low
column in U.S. EPA Method 300.0 (A). ammonium
IonPac 3 × 250 mm Hydroxide Recommended column for phosphoric 7.5 µm 55% - - 55 µeq Alkanol Ultralow
Fast and citric acids in cola soft drinks. quaternary
Anion ammonium
IIIA
IonPac 4 × 250 mm Specialty Polyvalent anions including chelating 10 µm 2% 530 5% 100 µeq Alkyl quaternary Medium-
AS7 Eluents agents, polyphosphates and nm ammonium High
polyphosphonates. Cyanide, sulfide,
hexavalent chromium, and arsenic
speciation.
IonPac 4 × 150 mm Hydroxide Low capacity for fast profiling of 5 µm 2% 60 4% 35 µeq Alkanol Low
AS5A organic acids and inorganic anions in nm quaternary
well-characterized samples. ammonium
IonPac 4 × 250 mm Hydroxide Metal-EDTA complexes, metal- 15 µm 2% 120 1% 20 µeq Alkanol Low
AS5 cyanide complexes, and oxyanions. nm quaternary
ammonium
312 www.dionex.com
IC Cation Columns
IC Cation Columns
Column Format Primary Application
Functional Group
Latex Diameter
Hydrophobicity
Eluent
(per column)
Crosslinking
Crosslinking
Substrate
Diameter
Capacity
Particle
Latex
IonPac 2 × 250 mm MSA Recommended column for polar 6 µm 55% - - 0.29 µeq Carboxylic Medium
CS18 amines (alkanolamines and acid
methylamines) and moderately
hydrophobic and polyvalent
amines (biogenic and diamines).
Nonsuppressed mode when
extended calibration linearity for
ammonium and weak bases is
required
IonPac 2 × 250 mm MSA Recommended column for 7 µm 55% - - 0.363 µeq Carboxylic Very Low
CS17 4 × 250 mm hydrophobic and polyvalent amines 1.45 µeq acid
(biogenic amines and diamines)
IonPac 3 × 250 mm MSA Recommended column for disparate 5 µm 55% - - 3.0 µeq Carboxylic Medium
CS16 5 × 250 mm concentration ratios of adjacent- 8.4 µeq acid
eluting cations such as sodium
and ammonium. Can be used for
alkylamines and alkanolamines.
IonPac 2 × 250 mm MSA Disparate concentration ratios 8.5 µm 55% - - 0.7 µeq Carboxylic Medium
CS15 4 × 250 mm of ammonium and sodium. Trace 2.8 µeq acid/
ethanolamine in high-ammonium phosphonic
or high- potassium concentrations. acid/ crown
Alkanolamines. ether
IonPac 2 × 250 mm MSA Aliphatic amines, aromatic amines, 8.5 µm 55% - - 0.325 µeq Carboxylic Low
CS14 4 × 250 mm and polyamines plus mono- and 1.3 µeq acid
divalent cations.
IonPac 2 × 100 mm MSA IC-MS screening column for fast 8.5 µm 55% - - 0.28 µeq Carboxylic Medium
CS12A- elution and low flow rates required acid/
MS for interfacing with IC-MS phosphonic
acid
IonPac 3 × 150 mm MSA Recommended column for high 5 µm 55% - - 0.94 µeq Carboxylic Medium
CS12A- efficiency and fast analysis (3 min) acid/

5 µm of mono- and divalent cations. phosphonic
acid
IonPac 2 × 250 mm MSA Recommended column for the 8.5 µm 55% - - 0.7 µeq Carboxylic Medium
CS12A 4 × 250 mm separation of mono- and divalent 2.8 µeq acid/
cations. Manganese morpholine, phosphonic
alkylamines, and aromatic amines. acid
IonPac 2 × 250 mm HCl + DAP Separation of mono- and divalent 8 µm 55% 200 nm 5% 0.035 µeq Sulfonic acid Medium
CS11 cations. Ethanolamines if divalent
cations are not present.
IonPac 4 × 250 mm HCl + DAP Separation of mono- and divalent 8.5 µm 55% 200 nm 5% 0.08 µeq Sulfonic acid Medium
CS10 cations.
IonPac 2 × 250 mm Pyridine Recommended column for transition 9 µm 55% 140 nm 10% 0.02 µeq/ Sulfonic -
CS5A 4 × 250 mm dicarboxylic and lanthanide metals analysis. 75 nm 20% 0.005 µeq acid/ alkanol
acid Aluminum analysis. 0.04 µeq/ quaternary
0.01 µeq ammonium
www.dionex.com 313
(per column)
Crosslinking
Crosslinking
Functional
Substrate
Diameter
Diameter
phobicity
Capacity
Column Format Primary Eluent Application
Particle
Hydro-
Group
Latex
Latex
IonPac 4 × 250 mm Heptafluorobutyric acid Organic acids in high 7.5 µm 8% - - 5.3 µeq Sulfonic acid Ultra Low
ICE-AS1 9 × 250 mm ionic strength matrices. 27 µeq
Fast separation of
organic acids.
IonPac 9 × 250 mm Heptafluorobutyric acid Organic acids in complex 8 µm 8% - - 27 µeq Sulfonic and Moderate
ICE-AS6 or high ionic strength carboxylic acid
matrices.
IonPac 9 × 250 mm MSA/ Mannitol Trace concentrations of 7.5 µm 8% - - 27 µeq Sulfonic acid Ultra Low
ICE- borate
Borate
Acclaim General and Specialty Columns
Explosives E1/2
Surfactant and
Organic Acid
Mixed-Mode
Mixed-Mode
Mixed-Mode
Advantage II
Advantage
120 C18
300 C18
120 C8
HILIC
Polar
Polar
WAX
WCX
(OA)
Bonded Phase Proprietary Proprietary Proprietary Proprietary Proprietary C18 C8 C18 Sulfamido Amide C18
alkyl amine alkyl diol alkyl carboxyl C16
USP Type na na na na na L1 L7 L1 na na
Endcapped Proprietary Proprietary Proprietary Yes Yes Yes Yes Yes Yes Yes
Substrate Ultrapure silica
Particle Shape Spherical
Particle Size 5 µm 5 µm 5 µm 5 µm 5 µm 2, 3 and 5 3 and 5 µm 3 µm 3 and 5 µm 2, 3 and 5
µm µm
Metal Impurity (ppm) <10 ppm
Na, Fe, Al
Average Pore Diameter 120 Å 120 Å 120 Å 120 Å 120 Å 120 Å 120 Å 300 Å 120 Å 120 Å
Surface Area (m /g)
2
300 300 300 300 300 300 300 100 300 300
Total Carbon Content na na na 17% na 18% 11% 7% 17% 17%
Bio Columns
Protein
Recommended
Backpressure
Compatibility
Crosslinking
Base Matrix
Column Phase Target Applications
Maximum
Flow Rate
pH Range
Substrate
Capacity
Material
Solvent
ProPac Weak High resolution and high 10-µm diameter 55% 6 mg/ mL 0.2–2 80% ACN, 3000 psi 2–12.0
WCX-10 Cation efficiency separations nonporous substrate lysozyme mL/min acetone. (21 MPa)
Exchange of proteins and to which is grafted a Incompatable
glycoproteins, pI =3-10, polymer chain bearing with alcohols
MW>10,000 units carboxylate groups. and MeOH
314 www.dionex.com
Bio Columns
Recommended
Backpressure
Compatibility
Crosslinking
Base Matrix
Column Phase Target Applications
Maximum
Flow Rate
pH Range
Substrate
Capacity
Material
Solvent
ProPac Strong High resolution and high 10 µm diameter 55% 3 mg/ mL 0.2–2.0 80% ACN, 3000 psi 2–12.0
SCX-10 Cation efficiency separations nonporous substrate lysozyme mL/min acetone, MeOH (21 MPa)
Exchange of proteins and to which is grafted a
glycoproteins, pI =3-10, polymer chain bearing
MW>10,000 units sulfonate groups.
ProPac Weak Anion High resolution and high 10 µm diameter 55% 5 mg/ mL 0.2–2.0 80% ACN, 3000 psi 2–12.0
WAX-10 Exchange efficiency separations non-porous substrate BSA/ mL mL/min acetone, (21 MPa)
of proteins and to which is grafted a MeOH,
glycoproteins, pI =3-10, polymer chain bearing
MW>10,000 units tertiary amine groups.
ProPac Strong High resolution and high 10 µm diameter non- 55% 15 mg/ mL 0.2–2.0 80% ACN, 3000 psi 2–12.0
SAX-10 Anion efficiency separations porous substrate with BSA mL/min acetone, MeOH (21 MPa)
Exchange of proteins and grafted polymer chain
glycoproteins, pI =3-10, bearing quaternary
MW>10,000 units ammonium groups.
ProSwift Reversed- Fast protein separation Monolith; polystyrene- Monolith 5.5 mg/mL 2–4 Most common 2800 psi 1–14
RP-1S Phase with high capacity using divinylbenzene with Standard Insulin mL/min organic solvents (19.2
Reversed Phase phenyl functional group permeability Mpa)
ProSwift Reversed- Fast protein separation Monolith; polystyrene- Monolith 1.0 mg/mL 1–10 Most common 2800 psi 1–14
RP-2H Phase with high capacity using divinylbenzene with High Lysozyme mL/min organic solvents (19.3
ProSwift Reversed- Fast protein separation Monolith; polystyrene- Monolith 0.5 mg/mL 1– 16 Most common 2800 psi 1–14
RP-3U Phase with high capacity using divinylbenzene with Ultrahigh Lysozyme mL/min organic solvents (19.3
ProSwift Strong Fast protein separation Monolith; polymethacry- Monolith 18 mg/mL 0.5–1.5 Most common 1000 psi 2–12.0
SAX-1S Anion with good resolution late with quaternary Standard BSA (4.6 mm), organic solvents (4.6 mm)
Exchange using Anion Exchange amine functional group permeability 2000 psi
0.05–.25 (1.0 mm)
(1.0 mm)
ProSwift Strong Fast protein separation Monolith; polymethacry- Monolith 30 mg/mL 0.5–1.5 Most common 1000 psi 2–12.0
SCX-1S Cation with good resolution late with sulfonic acid Standard Lysozyme mL/min organic solvents (4.6 mm)
Exchange using Cation Exchange fuctional group permeability (4.6 mm)

ProSwift Weak Anion Fast protein separation Monolith; Monolith 18 mg/mL 0.5–1.5 Most common 1000 psi 2–12.0
WAX-1S Exchange with good resolution polymethacrylate with Standard BSA mL/min organic solvents (4.6 mm)
using Anion Exchange tertiary amine (DEAE) permeability (4.6 mm), 2000 psi
functional group (1.0 mm)
0.05–.25
(1.0 mm)
ProSwift Weak Fast protein separation Monolith; Monolith 23 mg/mL 0.5–1.5 Most common 1000 psi 2–12.0
WCX-1S Cation with good resolution polymethacrylate with Standard Lysozyme mL/min organic solvents (4.6 mm)
Exchange using Cation Exchange carboxylic acid (CM) permeability (4.6 mm), 2000 psi
functional group (1.0 mm)
0.05–.20
(1.0 mm)
ProPac Immobilized High resolution 10 µm diameter non- 55% >60 mg 1.0 EtOH, urea, 3000 psi 2–12
IMAC-10 Metal separation of certain porous polystyrene lysozyme/ mL/min NaCl, nonionic (21MPa)
Affinity metal-binding proteins divinylbenzene substrate mL gel (4 x detergents,
and peptides with poly (IDA) grafts. 250 mm) glycerol, acetic
acid, guanidine
HCl
ProPac Reversed- Protein separation using Spherical 5 µm, ultrapure n/a 340 mg 1.0 2M Ammonium 4,000 psi 2.5–7.5
HIC-10 Phase hydrophobic interaction silica, 300 A, surface lysozyme per mL/ min sulfate/
with salt gradient elution area 100 m2/ g, 7.8 x 75 mm phosphate
column salts, organic
solvent for
cleanup
www.dionex.com 315
Carbohydrate
Recommended
Recommended
Backpressure
Compatibility
Crosslinking
Crosslinking
Target
Maximum
Flow Rate
pH Range
Substrate
Column Base Matrix Material
Capacity
Applications
Solvent
Eluents
Latex
CarboPac Reduced mono- 7.5-µm diameter macroporous 15% No latex 1450 µeq Hydroxide 0.4 0% 2000 psi 0–14
MA1 and disaccharide substrate fully functionalized (4 × 250 mm) mL/min (14 MPa)
analysis. with an alkyl quaternary
ammonium group
CarboPac General purpose 10-µm diameter non-porous 2% 5% 100 µeq Hydroxide, 1.0 0–5% 4000 psi 0–14
PA1 mono-, di and substrate agglomerted with a (4 × 250 mm) acetate/ mL/min (28 MPa)
oligosaccharide 500-nm MicroBead quaternary hydroxide
analysis ammonium functionalized latex
CarboPac Monosaccharide 10-µm diameter nonporous 55% 5% 100 µeq Hydroxide, 1.0 0–90% 3500 psi 0–14
PA10 compositonal substrate agglomerated with (4 × 250 mm) acetate/ mL/min (24.5
anaylysis a 460nm MicroBead di- hydroxide MPa)
functionalized latex
CarboPac Fast mono-, and 6.5-µm diameter nonporous 55% 5% 65 µeq Hydroxide, 0.5 0–100% 3000 psi 0–14
PA20 disaccharide substrate agglomerated with a (3 × 150 mm) acetate/ mL/min (21 MPa)
analysis 130-nm MicroBead quaternary hydroxide
ammonium functionalized latex
CarboPac Oligosaccharide 8.5-µm diameter nonporous 55% 6% 90 µeq Hydroxide, 1.0 0–90% 4000 psi 0–14
PA100 mapping and substrate agglomerated with (4 × 250 mm) acetate/ mL/min (28 MPa)
analysis a 275-nm MicroBead di- hydroxide
functionalized latex
CarboPac High resolution 5.5-µm diameter nonporous 55% 6% 35 µeq Hydroxide, 0.5 0–100% 4000 psi 0–14
PA200 oligosaccharide substrate agglomerated with a (3 × 250 mm) acetate/ mL/min (28 MPa)
mapping and 43-nm MicroBead quaternary hydroxide
analysis ammonium functionalized latex
DNA
Recommended
Recommended
Backpressure
Compatibility
Crosslinking
Crosslinking
Flow Rate
pH Range
Substrate
Column Target Applications Base Matrix Material

Capacity
Solvent
Eluents
Latex
Max.
DNAPac Single stranded DNA or 13-µm diameter nonporous 55% 5% 40 µeq Chloride, 1.5 0–100% 4000psi 2–12.5
PA100 RNA oligonucleotides, substrate agglomerated acetate, mL/min (28MPa)
restriction fragments, with a 100-nm MicroBead bromide,
glycoprotein isoforms. alkyl quaternary ammonium perchlorate:
functionalized latex. in lithium
sodium or
ammonium
forms
DNAPac High resolution single 8-µm diameter nonporous 55% 5% 40 µeq Chloride, 1.2 0–100% 4000psi 2–12.5
PA200 stranded DNA or RNA substrate agglomerated acetate, mL/min (28MPa)
oligonucleotides, with a 130-nm MicroBead bromide,
restriction fragments, alkyl quaternary ammonium perchlorate:
glycoprotein isoforms. functionalized latex. in lithium
sodium or
ammonium
forms
316 www.dionex.com
Dionex Literature
The following literature is available in Adobe PDF format on
www.dionex.com, by product category under Literature or via
search for the literature number shown below.
General Interest
Dionex Corporation Brochure................................................ 1993
Advances in Chemical Suppression....................................... 1855 UltiMate 3000 Proteomics MDLC Systems.......................... 2013
Reference Library CD-ROM.............................................. 053891 UltiMate 3000 RSLC Systems............................................... 2064
Virtual Column (Chromeleon) .............................................. 1614

Applications Booklets/CD-ROMs/s
Dionex LC/MS Applications Guide....................................... 1955 Accelerated Solvent Extraction (ASE)

Environmental Analysis Using ICS‑3000.............................. 1783 ASE 150 and 350 Accelerated Solvent Extractors
Brochure................................................................................. 2012
ICS‑3000 Solutions for the Semiconductor and
Electronic Industries............................................................... 1771 ASE 100/200/300 Brochure................................................... 1449
ICS‑3000 Solutions for the Power Industry........................... 1772
Determination of Bromate in Water Using Ion

Product Data Sheets
Chromatography..................................................................... 1970
Carbohydrate Analysis for the Food and IC/HPLC Product Data Sheets
Beverage Industry................................................................... 1971
ACC-3000 Autosampler Column Compartment................... 1964
Product Brochures APS Series Autopurification Systems.................................... 1606
AS40 Automated Sampler...................................................... 0539

IC/HPLC Products AXP Auxiliary Pump ............................................................ 1764

APS Series Autopurification Systems . ................................. 1621 AXP-MS Auxillary Pump for Mass Spectrometry................ 1884
IC Autosampler ..................................................................... 1751 ICS‑1000 Ion Chromatography System ............................... 1498
Chromeleon 6 ........................................................................ 1172 ICS‑1500 Ion Chromatography System ............................... 1499
Chromeleon IC....................................................................... 1591 ICS‑2000 Ion Chromatography System ............................... 1500
DX‑800 Process Analyzer ..................................................... 0956 ICS‑3000 Ion Chromatography System................................. 1683
ICS‑900 IC System ............................................................... 2003 MSQ Plus Mass Spectrometric Detector............................... 1453
ICS‑1000/1500/2000 Systems .............................................. 1514 MSQ18LA Nitrogen Gas Generator ..................................... 1543
ICS‑3000 IC System............................................................... 1691 PC10 Postcolumn Delivery System....................................... 1711
Probot MALDI Spotter.......................................................... 1506 ICS Series Photodiode Array Detector ................................. 1169
Reagent-Free IC (RFIC) Systems.......................................... 1819 Reagent-Free Controller (RFC‑30, RFC‑10) . ...................... 1494
UltiMate 3000 Basic Automated System............................... 1972 Reagent-Free IC with Eluent Generation: RFIC-EG ........... 1624
UltiMate 3000 HPLC and LC/MS System ........................... 1820 Reagent-Free IC with Eluent Regeneration: RFIC-ER......... 2022
www.dionex.com 317
Dionex Literature
Simultaneous Injection AS Autosampler for IC.................... 1607 Acclaim Surfactant................................................................. 1679
UltiMate 3000 Autosampler Series . ..................................... 1877 CarboPac MA1 .................................................................. 034796
UltiMate 3000 Diode Array Detector ................................... 1905 CarboPac PA1, PA10 & AminoTrap and BorateTrap .......... 1168
UltiMate 3000 Rapid Separation LC Systems....................... 2068 CarboPac PA20....................................................................... 1501
UltiMate 3000 Series Pump Systems ................................... 1818 CarboPac PA100................................................................. 034465
UltiMate 3000 Solvent Racks ............................................... 1874 CarboPac PA200..................................................................... 1618
UltiMate 3000 Thermostatted Column Compartment........... 1867 Concentrator and Trap Columns............................................ 0913
UltiMate 3000 Titanium Systems.......................................... 2040 Disposable Electrodes for Electrochemistry.......................... 1883
VWD-3000 Series Detectors.................................................. 1947 DNAPac PA100.................................................................. 034444
DNAPac PA200...................................................................... 1673

Accelerated Solvent Extraction (ASE)
IonPac AS4A-SC................................................................ 034638
ASE 100 Acccelerated Solvent Extractor ............................. 1450 IonPac AS5......................................................................... 032269
ASE 150 Acccelerated Solvent Extractor ............................. 2060 IonPac AS7............................................................................. 0843
ASE 200 Accelerated Solvent Extractor . ............................. 0639 IonPac AS9-SC and AS9-HC................................................. 0912
ASE 200 Solvent Controller, AutoASE................................. 0981 IonPac AS10 and IonPac AC10 ........................................ 034541
ASE 300 Accelerated Solvent Extractor . ............................. 1221 IonPac AS11/AS11-HC...................................................... 034827
ASE 350 Acccelerated Solvent Extractor ............................. 2061 IonPac AS12A........................................................................ 0533
SE 400 and SE 500 Solvent Evaporators . ............................ 1685 IonPac AS14........................................................................... 0744
IonPac AS14A........................................................................ 1213

Automation & Software
IonPac AS15........................................................................... 1000
AutoPrep System for Trace Analysis..................................... 1919
IonPac AS16........................................................................... 1074
Chromeleon 6 Computer Requirements................................ 0911
IonPac AS17-C....................................................................... 1912

Chromeleon PA ..................................................................... 1622
IonPac AS18........................................................................... 1513
Dionex Columns IonPac AS19........................................................................... 1616
IonPac AS20........................................................................... 1682

Acclaim 120 HPLC................................................................ 1413
IonPac AS21........................................................................... 1674
Acclaim 300 HPLC................................................................ 1511
IonPac AS22........................................................................... 1816
Acclaim Explosives................................................................ 1804
IonPac AS23........................................................................... 1817
Acclaim Organic Acid (OA).................................................. 1623
IonPac AS24........................................................................... 1974
Acclaim Mixed-Mode HILIC-1............................................. 1963
IonPac Cryptand C1 Concentrator......................................... 1678
Acclaim Mixed-Mode WAX-1............................................... 1899
IonPac Fast Anion IIIA........................................................... 1878
Acclaim Mixed-Mode WCX-1.............................................. 2024
IonPac CS5A.......................................................................... 0745
Acclaim Rapid Separation LC (RSLC)................................. 1923
IonPac CS10....................................................................... 034396
Acclaim PolarAdvantage (PA) HPLC................................... 1533
IonPac CS12....................................................................... 034687
Acclaim PolarAdvantage II (PA2) HPLC.............................. 1680
318 www.dionex.com
Application Notes
IonPac CS12A........................................................................ 0643 AN 21: Organic Acids in Wine.......................................... 032025
IonPac CS14....................................................................... 034890 AN 25: Determination of Inorganic Ions and Organic Acids in
Non-Alcoholic Carbonated Beverages.................................. 0662
IonPac CS15........................................................................... 0915
AN 31: Determination of Anions in Acid Rain
IonPac CS16........................................................................... 1257 by Ion Chromatography..................................................... 032133
IonPac CS17........................................................................... 1484 AN 36: Determination of Oxalate in Urine
by Ion Chromatography..................................................... 034797
IonPac CS18 .......................................................................... 1805
AN 37: Determination of Iodide in Milk Products................ 0702
IonPac ICE-AS1 Ion-Exclusion Column............................... 0814
AN 45: Fatty Acid Analysis............................................... 032292
IonPac ICE-AS6 Ion-Exclusion Column............................... 0546
IonPac NS1............................................................................. 0844
Analysis of Beer..................................................................... 0661
IonPac SCS 1 Silica Cation Separator................................... 1568
AN 51: Method for Determination of Anions in Sodium
IonPac TBC-1 Borate Concentrator Column and Hydroxide............................................................................... 1552
IonPac ICE-Borate Column................................................... 1001
AN 53: Method for the Determination of Trace Sulfate
ProPac Ion-Exchange Columns for Protein Analysis............ 1253 in Brine............................................................................... 032790
ProPac HIC‑10........................................................................ 1784 AN 54: Determination of Sulfite in Food and Beverages by Ion
Exclusion Chromatography with Pulsed Amperometric
ProPac IMAC‑10.................................................................... 1731 Detection............................................................................. 034004
ProSwift and PepSwift Monolith Columns........................... 1823 AN 55: Determination of Metal Cyanides......................... 034055
ProSwift Ion Exchange Monolith Columns........................... 2020 AN 56: Determination of Trace Anions and Key Organic Acids
in High Purity, Ammoniated, and Borated Waters Found in
Steam Cycle Power Plants ................................................ 034074
Suppressors
AN 61: Tobramycin in Pharmaceutical Formulations........... 1626
Carbonate Removal Device (CRD) 200 for
RFIC Systems......................................................................... 1786 AN 65: Analysis of Inositol Phosphates............................ 034407
Carbonate Removal Device (CRD) 300 for AN 66: Neomycin in Topical Lotions.................................... 0899
Carbonate Eluents................................................................... 1978 AN 67: Determination of Plant-Derived Neutral Oligo- and

Eluent Suppressors for Ion Chromatography........................ 1290 Polysaccharides...................................................................... 1474
Salt Converter-Cation Self-Regenerating Suppressor AN 68: Determination of Inorganic Anions Using Capillary
(SC-CSRS)............................................................................. 1456 Zone Electrophoresis.......................................................... 034510
AN 69: Determination of Aluminum in Complex Matrices

Using Chelation Ion Chromatography............................... 034532
Reagents, Standards, and Accessories
AN 70: Choline and Acetylcholine.................................... 034516
IC Standards & Reagents................................................... 034116
AN 71: Determination of Polyphosphates Using Ion Chroma-
SE Prep CR Cation-Exchange Resin for Use with tography with Suppressed Conductivity Detection........... 034629
ASE 150 and 350.................................................................... 2071
AN 72: Determination of Trace Metals in Water Miscible
Organic Solvents by Ion Chromatography/Inductively Coupled
Application Notes Argon Plasma Spectroscopy (IC/ICAP)............................ 034619
AN 73: Determination of Trace Transition Metals in Reagent

IC/HPLC Application Notes Grade Acids, Bases, and Salts Using Ion Chromatography/
Inductively Coupled Argon Plasma Spectroscopy
AN 2: Analysis of Nitrate and Sulfate Collected on (IC/ICAP)........................................................................... 034620
Air Filters............................................................................ 034635
www.dionex.com 319
Dionex Literature
AN 75: Determination of Trace Transition Metals in Reagent AN 97: Determination of Carbonyl Compounds by Reversed-
Grade Acids, Bases, Salts, and Organic Solvents Using Phase High-Performance Liquid Chromatography............... 0578
Chelation Ion Chromatography.......................................... 034659
AN 99: Peptide Mapping by Reversed-Phase
AN 76: Elimination of Iron and Aluminum Interferences in High-Performance Liquid Chromatography.......................... 0581
Sample Matrices by Ion Chromatography/Inductively Coupled
Argon Plasma Emission Spectroscopy (IC/ICAP)............ 034658 AN 100: High-Resolution Analysis and Purification
of Oligonucleotides................................................................ 0591
AN 77: Elimination of Iron and Aluminum as Matrix Interfer-
ences for Determination of Transition Metals Using Chelation AN 101: Trace Level Determination of Bromate in Ozonated
Ion Chromatography.......................................................... 034660 Drinking Water Using Ion Chromatography......................... 0623
AN 78: Determination of Trace Anions in AN 102: Microbore Peptide Mapping with the DX‑500....... 0625
Concentrated Hydrofluoric Acid........................................ 034669
AN 104: Analysis of Personal Care Products by IC.............. 0657
AN 79: Determination of Uranium and Thorium in Complex
AN 105: Glycosylation Analysis of Human Serum Transferrin
Matrices Using Chelation Ion Chromatography................ 034670
Glycoforms Using Pellicular Anion-Exchange
AN 80: Determination of Dissolved Hexavalent Chromium in Chromatography..................................................................... 0658
Drinking Water, Groundwater, and Industrial Wastewater Ef-
AN 106: Ion Chromatography in the Pharmaceutical
fluents by Ion Chromatography......................................... 034685
Industry................................................................................... 0660
AN 81: Ion Chromatographic Determination of Oxyhalides
AN 107: Ions in Physiological Fluids.................................... 0675
and Bromide at Trace Level Concentrations in
Drinking Water Using Direct Injection ................................. 0965 AN 108: Determination of Transition Metals in Serum and
Whole Blood by Ion Chromatography.................................. 0692
Sugar from Beets................................................................ 034751 AN 109: Determination of Glyphosate by Cation-Exchange
Chromatography with Postcolumn Derivatization................ 0663
AN 83: Size-Exclusion Chromatography of Polysaccharides
with Pulsed Amperometric Detection................................ 034801 AN 112: Determination of Nitrate and Nitrite in
Meat Using HPAE Chromatography..................................... 0837
AN 85: Determination of Trace Anions in Organic
Solvents................................................................................... 0482 AN 113: Determination of Trace Anions in High-Purity
Waters by High Volume/Direct Injection IC.......................... 0736
AN 86: Determination of Trace Cations in Power Plant Waters
Containing Morpholine.......................................................... 0483 AN 114: Determination of Trace Anions in High-Purity Waters
Using Direct Injection and Two-Step Isocratic IC................. 0883
AN 87: Determination of Sugar Alcohols in Confections and
Fruit Juices by High-Performance Anion Exchange AN 115: Determination of Trifluoroacetic Acid (TFA)
Chromatography with Pulsed Amperometric Detection....... 0534 in Peptides............................................................................... 0898

AN 88: Medium-Pressure Gel Filtration Chromatography AN 116: Quantification of Anions In Pharmaceuticals......... 0924
Using the Dionex DX‑500 HPLC System............................. 0540
AN 117: Quantification of Carbohydrates
AN 92: Determination of Sugars in Molasses by High- in Pharmaceuticals.................................................................. 0957
Performance Anion Exchange with Pulsed Amperometric
Detection................................................................................. 0565 AN 119: Determination of an Anionic Fluorochemical Surfac-
tant in a Semiconductor Etch Bath......................................... 0959
Using AutoNeutralization Pretreatment/Ion AN 120: Determination of Calcium and Magnesium
Chromatography..................................................................... 1633 in Brine................................................................................... 0990
AN 94: Determination of Trace Cations in Concentrated Acids AN 121: Analysis of Low Concentrations of Perchlorate in
Using AutoNeutralization Pretreatment/Ion Drinking Water and Groundwater by IC................................ 0991
Chromatography..................................................................... 1666
AN 122: Determination of Carbohydrates, Alcohols,
AN 95: Polycyclic Aromatic Hydrocarbon Determination by and Glycols in Fermentation Broths...................................... 1029
Reversed-Phase High-Performance Liquid
Chromatography..................................................................... 0570 AN 123: Determination of Inorganic Anions and Organic Acids
in Fermentation Broths........................................................... 1030
AN 96: Determination of N-Methylcarbamates by Reversed-
Phase High-Performance Liquid Chromatography............... 0571 AN 124: Determination of Choline in Milk and Infant
Formula................................................................................... 1054
320 www.dionex.com
Application Notes
AN 125: Monitoring Protein Deamidation by Cation-Exchange AN 145: Determination of the Suppressor Additive in Acid
Chromatography: Rate of Forced Deamidation Copper Plating Bath .............................................................. 1473
of Asparagine Residues in Ribonuclease A........................... 1045
AN 146: Determination of Trace Anions in High-Purity Waters
AN 126: Analysis of Hemoglobin Variants by by Ion Chromatography with the IonPac AS17 Using High-
Cation-Exchange Chromatography........................................ 1046 Volume Direct Injection with the EG40................................. 1507
AN 127: Analysis of Monoclonal Antibody Heterogeneity AN147: Determination of Polydextrose in Foods by AOAC
by Cation-Exchange Chromatography: Separation of Method 2000.11 .................................................................... 1528
C-Terminal Lysine Variants.................................................... 1047
AN 148: Determination of Bethanechol by
AN 128: Monitoring Stability of Monoclonal Antibodies Ion Chromatography.............................................................. 1510
by Cation-Exchange Chromatography.................................. 1052
AN 149: Determination of Chlorite, Bromate, Bromide, and
AN 129: Separation of Tryptophan and Methionine Oxidized Chlorate in Drinking Water by Ion Chromatography with an On-
Peptides From Their Unoxidized Forms................................ 1053 Line Generated Postcolumn Reagent for Sub-µg/L
Bromate Analysis................................................................... 1523
AN 130: Identification of a Hydroxylysine-Containing Peptide
Using AAA-Direct.................................................................. 1504 AN 150: Determination of Amino Acids in Cell Cultures and
Fermentation Broths............................................................... 1538
AN 131: Determination of Transition Metals at PPT Levels in
High-Purity Water and SC-2 (D-Clean) Baths...................... 1058 AN 151: Determination of Perchlorate in Environmental Waters
by Ion Chromatography Coupled with Electrospray
AN 132: Determination of Sulfur-Containing Antibiotics Using Mass Spectrometry................................................................. 1496
Integrated Pulsed Amperometric Detection (IPAD).............. 1126
AN 152: Determination of Sodium at the Parts-Per-Trillion
AN 133: Determination of Inorganic Anions in Drinking Water Level in the Presence of High Concentrations
by Ion Chromatography......................................................... 1192 of Ethanolamine..................................................................... 1526
AN 134: Determination of Low Concentrations of Perchlorate AN 153: Monitoring for Trace Anion Contamination in the
in Drinking and Ground Waters Using Extracts of Electronic Components....................................... 1527
Ion Chromatography.............................................................. 1194
AN 154: Determination of Inorganic Anions in Environmental
AN 135: Determination of Inorganic Anions in Wastewater by Waters Using a Hydroxide-Selective Columns..................... 1539
Ion Chromatography.............................................................. 1226
AN 155: Determination of Trans Galactooligosaccharides in
AN 136: Determination of Inorganic Oxyhalide Disinfection Foods by AOAC Method 2001.02.......................................... 1547
By-product Anions and Bromide in Drinking Water Using Ion
Chromatography with the Addition of a Postcolumn Reagent for AN 156: Determination of Anions in Toothpaste by
Trace Bromate Analysis......................................................... 1229 Ion Chromatography.............................................................. 1548

AN 137: Determination of Trace Anions in High-Nitrate Matri- AN 157: Comparison of Suppressed to Nonsuppressed Conduc-
ces by Ion Chromatography................................................... 1233 tivity Detection for the Determination of Common
Inorganic Cations.................................................................... 1560
AN 138: Determination of Thiosulfate in Refinery and
Other Wastewaters.................................................................. 1237 AN 158: Determination of Trace Sodium and Transition Metals
in Power Industry Samples by Ion Chromatography with Non-
AN 139: Determination of Additives and Byproducts in an Acid suppressed Conductivity Detection........................................ 1569
Copper Plating Bath by Liquid Chromatography.................. 1251
AN 159: Determination of Sucralose Using HPAE-PAD..... 1574
AN 140: Fast Analysis of Anions in Drinking Water by Ion
Chromatography..................................................................... 1295 AN 160: Determination of Residual Trifuoroacetate in Protein
Purification Buffers and Peptide Preparation by Reagent-Free
AN 141: Determination of Inorganic Cations and Ammonium in Ion Chromatography.............................................................. 1627
Environmental Waters by Ion Chromatography
Using the IonPac CS16 Column............................................ 1404 AN 161: Determination of Metal Cyanide Complexes by Ion
Chromatography with On-Line Sample Preconcentration
AN 142: Determination of Tryptophan Using AAA-Direct.. 1408 and UV Absorbance Detection............................................... 1628
AN 143: Determination of Organic Acids in Fruit Juices..... 1415 AN 162: Determination of Nucleotides by Ion Chromatography
with UV Absorbance Detection............................................. 1631
AN 144: Determination of Perchlorate in High Ionic Strength
Fertilizer Extracts by Using Ion Chromatography................ 1425 AN 163: Determination of Protein Concentration Using
AAA-Direct............................................................................ 1634
www.dionex.com 321
Dionex Literature
AN 164: Assay for Citrate and Phosphate in Pharmaceutical AN 181: Determination of Streptomycin and Impurities Using
Formulations Using Ion Chromatography............................. 1643 HPAE-PAD............................................................................. 1887
AN 165: Determination of Benzoate in Liquid Food Products by AN 182: Determination of Biogenic Amines in Alcoholic Bev-
Reagent-Free Ion Chromatography....................................... 1645 erages by Ion Chromatography with Suppressed Conductivity
and Integrated Pulsed Amperometric Detections.................. 1888
AN 166: Application of Eluent Generation for Trace Anion
Analysis of Borated Waters.................................................... 1654 AN 183: Determination of Biogenic Amines in Fermented and
Non-Fermented Foods Using Ion Chromatography with Sup-
AN 167: Determination of Trace Concentrations of Oxyhalides pressed Conductivity and Integrated Pulsed Amperometric
and Bromide in Municipal and Bottled Waters Using a Hydrox- Detections............................................................................... 1889
ide-Selective Column with a Reagent-Free
Ion Chromatography System................................................. 1662 AN 184: Determination of Trace Concentrations of Chlorite,
Bromate, and Chlorate in Bottled Natural Mineral Waters... 1890
AN 168: Determination of Trace Concentrations of Disinfection
By-Product Anions and Bromide in Drinking Water Using AN 185: Determination of Trace Concentrations of Organic
Reagent-Free Ion Chromatography Followed by Postcolumn Acids and Inorganic Anions in Boric Acid-Treated Power
Addition of o-Dianisidine for Trace Bromate Analysis......... 1706 Plant Waters Using an Automated Reagent-Free Ion
Chromatography Method....................................................... 1996
AN 169: Rapid Determination of Phosphate and Citrate in
Carbonated Soft Drinks Using a Reagent-Free Ion AN 186: Analysis of Paromomycin by HPAE-IPAD............ 1942
Chromatography System........................................................ 1774
AN 187: Determination of Sub-μg/L Bromate in Municipal and
AN 170: Determination of Silicate in High Purity Water Natural Mineral Waters Using Preconcentration with Two-
Using Ion Chromatography and AutoPrep............................ 1992 Dimensional Ion Chromatography and Suppressed
Conductivity Detection........................................................... 1943
Bromide in Drinking Water Using a Reagent-Free Ion AN 188: Determination of Glycols and Alcohols in Fermenta-
Chromatography System Followed by Postcolumn Addition tion Broths Using Ion-Exclusion Chromatogrpahy and
of an Acidified On-Line Generated Reagent for Trace Pulsed Amperometric Detection............................................ 1944
Bromate Analysis................................................................... 1767
AN 189: Determination of Explosive Compounds in Drinking
AN 172: Determination of Azide in Aqueous Samples by Ion Water Using Parallel-HPLC with UV Detection................... 1945
Chromatography with Suppressed Conductivity Detection.. 1831
AN 190: Determination of Sulfate Counter Ion and Anionic
AN 173: Direct Determination of Cyanide in Drinking Water by Impurities in Aminoglycoside Drug Substances by Ion Chroma-
Ion Chromatography with Pulsed Amperometric Detection.1787 tography with Suppressed Conductivity Detection............... 1946
AN 174: Calculating Instrument Utilization Using AN 191: Determination of Phenols in Drinking and Bottled
Chromeleon............................................................................ 1777 Mineral Waters Using Online Solid-Phase Extraction
Followed by HPLC with UV Detection................................. 1949

AN 175: Determination of Sulfate and Chloride in Ethanol by
Ion Chromatography.............................................................. 1827 AN 192: Rapid Analysis of Ginseng Using Accelerated
Solvent Extraction and HPLC................................................ 1965
AN 176: Determining Sub-ppb Perchlorate in Drinking Water
Using Preconcentration/Matrix Elimination Ion Chromatogra- AN 193: Determination of Additives in Carbonated
phy with Suppressed Conductivity Detection by Beverages................................................................................ 1961
U.S. EPA Method 314.1......................................................... 1792
AN 194: Determination of Carbachol in Opthalmic Solutions
AN 177: Separation of an Intact Monoclonal Antibody and Using a Reagent-Free Ion Chromatography System............. 1967
Fractionation of Monoclonal Antibody Papain Digest Fragments
Using Immobilized Metal Affinity Chromatography............ 1840 AN 195: Determination of Verapamil Hydrochloride Purity
Using the Acclaim PA Column.............................................. 2039
AN 178: Improved Determination of Trace Concentrations of
Perchlorate in Drinking Water Using Preconcentration with AN 196: Determination of Polycyclic Aromatic Hydrocarbons
Two-Dimensional Ion Chromatography and Suppressed (PAHs) in Edible Oils by Donor-Acceptor Complex Chroma-
Conductivity Detection........................................................... 1835 tography (DACC)-HPLC with Fluorescence Detection........ 1998
AN 179: Carbohydrate and Amino Acid Analysis Using AN 197: Determination of Glucosamine in Dietary
3-D Amperometry.................................................................. 1839 Supplements Using HPAE-PAD............................................ 2001
AN 180: Determination of Nevirapine Using HPLC with AN 199: Determination of N-Methylpyrrolidine in Cefepime
UV Detection.......................................................................... 1934 Using a Reagent-Free Ion Chromatography System............. 2005
322 www.dionex.com
Application Notes
AN 200: Direct Determination of Cyanate in a Urea Solution AN 334: Rapid Determination of Fat in Meat by
and a Urea-Containing Protein Buffer................................... 2034 Accelerated Solvent Extraction (ASE).................................. 1125
AN 335: Accelerated Solvent Extraction (ASE) of Active

ASE Application Notes Ingredients from Natural Products......................................... 1115
AN 313: Extraction of PAHs from Environmental Samples AN 336: Accelerated Solvent Extraction (ASE) of Plasticizers
by Accelerated Solvent Extraction (ASE)............................. 0632 from Poly(vinyl chloride) Polymer........................................ 1117
AN 316: Extraction of PCBs from Environmental Samples AN 337: Extraction of Lipids and Polychlorinated Biphenyls
Using Accelerated Solvent Extraction (ASE)........................ 0633 from Fish Tissue in a Single Run Using ASE........................ 1118
AN 317: Extraction of BNA (Bases, Neutrals, and Acids) AN 338: Extraction of Total Petroleum Hydrocarbon Contami-
Using Accelerated Solvent Extraction (ASE)........................ 0634 nants (Diesel and Waste Oil) in Soils by Accelerated Solvent
Extraction (ASE).................................................................... 1167
AN 318: Extraction of Chlorinated Herbicides Using
Accelerated Solvent Extraction (ASE).................................. 0635 AN 339: Determination of Organotin Compounds in Sediments
Using Accelerated Solvent Extraction (ASE)........................ 1184
AN 319: Extraction of Organophosphorus Pesticides
Using Accelerated Solvent Extraction (ASE)........................ 0636 AN 340: Determination of Fat in Dried Milk Products by
Accelerated Solvent Extraction (ASE).................................. 1185
AN 320: Extraction of Chlorinated Pesticides Using
Accelerated Solvent Extraction (ASE).................................. 0637 AN 341: Extraction of BNAs from Large-Volume Samples
AN 321: Determination of Unbound Fat in
Various Food Matrices Using ASE........................................ 0763 AN 342: Determination of PCBs in Large-Volume Fish Tissue
Samples Using Accelerated Solvent Extraction (ASE)......... 1204
AN 322: Selective Extraction of PCBs from Fish Tissue
Using Accelerated Solvent Extraction (ASE)........................ 0764 AN 343: Determination of Pesticides in Large-Volume Fish Tis-
sue Samples Using Accelerated Solvent Extraction (ASE)... 1205
AN 323: Extraction of Polychlorinated Dibenzo-p-dioxins
and Polychlorinated Dibenzofurans from AN 344: Extraction of Fat from Chocolate Using Accelerated
Environmental Samples Using ASE...................................... 0812 Solvent Extraction (ASE)....................................................... 1230
AN 324: Accelerated Solvent Extraction (ASE) of Hydrocarbon AN 345: Extraction of Fat from Diary Products (Cheese, Butter,
Contaminants (BTEX, Diesel, and TPH) in Soils................. 0731 and Liquid Milks) Using Accelerated Solvent
Extraction (ASE).................................................................... 1232
AN 325: Extraction of Oils from Oilseeds Using
Accelerated Solvent Extraction (ASE).................................. 0786 AN 346: Totally Automated Sample Preparation Using Acceler-
ated Solvent Extraction (ASE) Coupled with Gilson ASPEC:

AN 326: Extraction of Drugs from Animal Feeds Using Accel- The Determination of Dianthrones in St. John’s Wort.......... 1410
erated Solvent Extraction (ASE)............................................ 1042
AN 347: Use of Accelerated Solvent Extraction (ASE) for
AN 327: Extraction of Nitroglycerin from Transdermal Patches Cleaning and Elution of XAD Resin..................................... 1424
by Accelerated Solvent Extraction (ASE)............................. 0860
AN 348: Extraction of Anthelmintic Drugs from Veterinary
AN 328: Extraction of Explosives from Soils by Formulation Using Accelerated Solvent Extraction (ASE)... 1573
AN 349: Rapid Determination of Organochlorine Pesticides in
AN 329: Determination of Fat in Powdered Infant Formula Animal Feed Using Accelerated Solvent Extraction (ASE).. 1575
AN 350: Rapid Determination of Zearalenone from Wheat and
AN 330: Accelerated Solvent Extraction (ASE) of the Organic Corn by Accelerated Solvent Extraction (ASE).................... 1609
Components of Granular and Liquid Detergents................... 0994
AN 351: Rapid Determination of Polybrominated Diphenyl
AN 331: Accelerated Solvent Extraction (ASE) Ethers (PBDEs) in Biosolids and Waste Samples Using Acceler-
of Additives from Polymer Materials..................................... 0987 ated Solvent Extraction (ASE)............................................... 1648
AN 332: Accelerated Solvent Extraction (ASE) of Pesticide AN 352: Rapid Determination of Persistent Organic Pollutants
and Herbicide Residues in Food............................................ 1043 (POPs) Using Accelerated Solvent Extraction (ASE)........... 1676
AN 333: Accelerated Solvent Extraction (ASE) of PCBs from AN 353: Rapid Determination of Sulfonamide Residues in Ani-
Polyurethane Foam (PUF) Adsorbent Cartridges.................. 1083 mal Tissue and Infant Food Containing Animal Products Using
www.dionex.com 323
Dionex Literature
AN 354: Accelerated Solvent Extraction (ASE) of Extractables AN 508: Analysis of Water Tracers Using On-line
from Polymeric Components Used in Precision Drug Preconcentration in Micro HPLC.......................................... 1433
Delivery Devices.................................................................... 1709
AN 509: Automated On-line SDS Removal from Minute
AN 355: Rapid Extraction and Determination of Arsenicals Proteinaceous Samples Prior to Capillary HPLC.................. 1434
in Fish Tissue and Plant Material Using Accelerated Solvent
Extraction (ASE).................................................................... 1782 AN 510: Fully Automated Handling of Minute
Sample Volumes..................................................................... 1435
AN 356: Determination of Perchlorate in Vegetation Samples
Using Accelerated Solvent Extraction (ASE) and AN 511: Direct Analysis of Anti-Epileptic Drugs from Serum
Ion Chromatography.............................................................. 1830 Using Microcolumn Switching/Capillary HPLC.................. 1436
AN: 357: Extraction of Phenolic Acids from Plant Tissue Using AN 512: Automated Sample Handling of Single Beads for the
Accelerated Solvent Extraction (ASE).................................. 1837 Development of Combinatorial Libraries.............................. 1437
AN 358: Extraction and Cleanup of Acrylamide in Complex AN 513: Automated Large and Extra-Large Volume Injections
Matrices Using Accelerated Solvent Extraction (ASE) Followed in Nanoscale HPLC................................................................ 1438
by Liquid Chromatography Tandem Mass Spectrometry
AN 514: Automated Peptide Extraction from 2-D Gel Protein
(LC-MS/MS).......................................................................... 1922
Spots....................................................................................... 1439
AN 359: Extraction of Contaminants, Pollutants, and Poisons
AN 515: Automated On-line Salt Removal in Micro HPLC
from Animal Tissue Using ASE............................................. 1966
ESI-MS................................................................................... 1440
AN 360: Rapid Determination of Azo Dyes in Textiles Using
AN 516: Microfraction for Automated 2-D Capillary LC.... 1441
ASE and UltiMate 3000 HPLC Systems with MSQ Plus
and UV Detection................................................................... 2007 AN 517: Sampling into Nano Electrospray Needles............. 1442
AN 361: Extraction of Total Fat from Food Samples after Acid AN 518: On-line Nano LC/Nanospray MS for the Analysis of
Hydrolysis Using ASE with GC-MS Analysis...................... 2008 Protein Digests........................................................................ 1443
AN 409: Fast Determination of Acrylamide in Food Samples AN 519: Parallel Sample Cleanup and Analysis
Using Accelerated Solvent Extraction (ASE) Followed in Capillary LC ...................................................................... 1444
by Ion Chromatography with UV or MS Detection.............. 1497
AN 520: Two-Dimensional Analysis of Isotope Coded Affinity
Tag (ICAT) Labeled Proteins................................................. 1446
Nano and Capillary LC Application
Notes AN 521: Automated 2-D LC Coupled to ESI-MS/MS for the
Analysis of Complex Peptide Sample................................... 1470
AN 500: Rapid Identification of Drug Metabolites Using AN 522: Monolithic Capillary Column in LC/MS
Capillary LC/MS/MS............................................................. 1426 Proteomics.............................................................................. 1597

AN 501: Turbulent Flow Capillary LC/MS/MS for Ultra-Fast AN 523: Fast Protein/Peptide Separations Using Monolithic
and Sensitive Pharmacokinetics............................................. 1427 Capillary Columns.................................................................. 1598
AN 502: Peak-Parking for Enhanced Protein/Peptide Analysis AN 524: Parallel Capillary/Nano LC for High-Throughput
in Nanospray LC/MS/MS...................................................... 1428 MS Proteomics....................................................................... 1599
AN 503: On-line Clean-Up and Preconcentration of Protein AN 525: LC-MALDI MS Using Monolithic
Samples Prior to Nanoscale LC/MS/MS............................... 1445 Capillary Columns.................................................................. 1641
AN 504: Analysis of Sumatriptan in Cerebro-Spinal Fluid AN 526: Monolithic Columns Applied in an Off-Line 2-D LC/
Using Capillary LC/MS/MS.................................................. 1429 ESI-MS Bottom-Up Study for the Identification of
Platelet Proteins...................................................................... 1770
AN 505: Identification of Tryptic Peptides of Protein C Inhibitor
Using Capillary LC/MS......................................................... 1430 AN 527: Comprehensive 2-D Nano LC/MS for Human
Tissue Proteomics................................................................... 1635
AN 506: Analysis of Phosphopeptides Using
Nano LC/MS/MS................................................................... 1431 AN 528: Parallel LC With Capillary PS-DVB Monolithic
Columns for High Throughput............................................... 1762
AN 507: Direct Injection of Concentrated Pharmaceutical
Formulations.......................................................................... 1432
324 www.dionex.com
Application Updates
AN 529: Capillary PS-DVB Monolithic Column of 500-mm i.d. AU 142: Improved Determination of Trace Anions in High
for Peptide and Protein Separations in Top-Down Purity Water by High-Volume Direct Injection with
Proteomics Studies................................................................. 1775 the EG40................................................................................. 1291
AN 530: Proteome Analysis Involving Off-Line 2-D LC of In- AU 143: Determination of Chloride in Acid Copper
tact Proteins, Proteolytic Digestion, and Capillary RP-LC-MS/ Plating Bath............................................................................ 1303
MS Analysis Using Monolithic PS-DVB Columns............... 1776
AU 144: Determination of Hexavalent Chromium in Drinking
AN 531: Phosphopeptide Enrichment Using a Water Using Ion Chromatography ........................................ 1495
TiO2 Nano Precolumn............................................................ 1898
AU 145: Determination of Perchlorate in Drinking Water
by Ion Chromatography......................................................... 1540
Application Updates
AU 146: Determination of Anions in Acid Rain
AU 102: Trace Anions in Power Plant High-Purity Water and by Ion Chromatography......................................................... 1541
Borated Water..................................................................... 032728 AU 147: Direct Determination of Metal Cyanides by
AU 107: Direct Determination of Cyanide Ion Chromatography with UV Absorbance Detection.......... 1570
in Strongly Alkaline Solutions............................................... 0754 AU 148: Determination of Perchlorate in Drinking Water Using
AU 109: Azole Corrosion Inhibitors.................................. 032787 Reagent-Free Ion Chromatography....................................... 1601
AU 111: Copper Gleam PCM or PC in LeaRonal Acid AU 149: Determination of Metal Cyanide Complexes in Solid
Copper Baths...................................................................... 032804 Wastes by Anion-Exchange Chromatography with UV
Absorbance Detection............................................................ 1651
AU 113: Determination of Silica....................................... 032824
AU 150: Determination of Plant-Derived Neutral Oligosaccha-
AU 119: Phenols................................................................. 032841 rides and Polysaccharides Using the CarboPac PA200......... 1779
AU 121: Monovalent Cations in Explosives...................... 034632 AU 151: Determination of Sucralose in Reduced-Carbohydrate

Colas Using High-Performance Anion-Exchange Chromatogra-
AU 122: Determination of Iodide in Brine........................ 034075 phy With Pulsed Amperometric Detection............................ 1766
AU 125: Monosaccharide Analysis of Serum.................... 034460 AU 152: An Improved Gradient Method for the AAA-Direct
Separation of Amino Acids and Carbohydrates in Complex
AU 126: Determination of Diethanolamine and Triethanolamine Sample Matrices..................................................................... 1768
in Surface Finishing, Wastewater,
and Scrubber Solutions....................................................... 034505 AU 153: Fast Determinations of Phosphate and Citrate in Car-
bonated Beverages Using On-Line Degassing with the Carbon-
AU 131: Determination of Nitrite and Nitrate in Drinking Water ate Removal Device (CRD) and a Reagent-Free

Using Chemically Suppressed Ion Chromatography........ 034504 Ion Chromatography System..........................................................
AU 132: Determination of Nitrite and Nitrate in Drinking Water AU 154: Determination of Bromate in Drinking and Mineral
Using IC with Direct UV Detection................................... 034527 Water by Isocratic Ion Chromatography with
AU 133: Saccharin in an Electrolytic Nickel Sulfate Hydroxide Eluent................................................................... 1793
Bath..................................................................................... 034621 AU 155: Determination of Cations and Amines in Hydrogen
AU 137: Determination of Trace Lithium in Peroxide by Ion Chromatography Using an RFIC
Industrial Process Waters....................................................... 0952 (Reagent-Free IC) System...................................................... 1832
AU 138: Determination of Ethanolamines in Industrial Waters AU 156: Evaluation of Acclaim HPLC Columns Using the
by Cation-Exchange Chromatography.................................. 0995 NIST Standard Reference Material 870................................. 1834
AU 139: Determination of an Anionic Fluorochemical AU 157: Using a Reagent-Free Ion Chromatography System to
Surfactant (FC‑95) in a Steel Bath......................................... 1002 Monitor Trace Anion Contamination in the Extracts of Elec-
tronic Components................................................................. 1853
AU 140: Determination of Iodide in Urine............................ 1035
AU 158: Determination of Manganese in Brine.................... 1891
AU 141: Improved Long-Term Stability of N-Acetylneuraminic
Acid and N-Glycolylneuraminic Acid Peak Area Responses Us- AU 159: Determination of Volcanic Gases as Anions in Caustic
ing Waveform A, a Quadruple Potential Waveform.............. 1225 Solutions Using AutoNeutralization, Automated Dilutions, and
a Reagent-Free Ion Chromatography System........................ 1892
www.dionex.com 325
Dionex Literature
AU 160: Determination of N,N-Dimethyl-o-Toluidine and TN 28: Ion Chromatography/Inductively Coupled Argon
N,N‑Diethyl-p-Toluidine in Ethylene Gas Samples.............. 1897 Plasma (IC/ICAP): A New Technique for
Trace Metal Determinations............................................... 034600
AU 161: Determination of Sulfate and Chloride in Ethanol Us-
ing Ion Chromatography........................................................ 1920 TN 29: Automated Sample Preconcentration of Metals in
Drinking Water or Inductively Coupled
AU 162: Determination of Biogenic Amines in Fruit, Veg- Argon Plasma (ICAP) Spectroscopy................................. 034838
etables, and Chocolate Using Ion Chromatography with
Suppressed Conductivity and Integrated Pulsed Amperometric TN 30: Monosaccharide and Oligosaccharide Analysis of
Detections .............................................................................. 1939 Glycoproteins Electrotransferred onto
Polyvinylidene Fluoride (PVDF) Membranes................... 034883
AU 163: Determination of Trace Anions in Organic Solvents
Using Matrix Elimination and Preconcentration................... 1962 TN 36: Analysis of Exoglycosidase Digestions of N-Linked
Oligosaccharides Using HPAE-PAD..................................... 0626
AU 164: Determination of Glucosamine in Chondroitin Sulfate-
Containing Dietary Supplements Using HPAE-PAD............ 2035 TN 40: Glycoprotein Monosaccharide Analysis Using
HPAE-PAD and Eluent Generation....................................... 1632
Technical Notes TN 41: Analysis of Sialic Acids Using High-pH

Anion-Exchange Chromatography........................................ 0931
IC/HPLC Technical Notes TN 42: Glycoprotein Oligosaccharide Analysis Using High-pH

Anion Exchange Chromatography......................................... 0932
TN 8: Use of Concentrator Columns in IC............................ 0576 TN 43: Using Smoothing Algorithms to Reduce Baseline
Noise in Chromatography...................................................... 1082
TN 9: Conductivity Detection, Conductance Laws, and
Electrolyte Equilibria......................................................... 032111 TN 44: Determination of Trace Anions in Concentrated
Phosphoric Acid..................................................................... 1084
TN 10: Determination of Transition Metals
by Ion Chromatography.................................................... 032229 TN 45: Determination of Trace Anions in Concentrated
Hydrofluoric Acid................................................................... 1106
TN 12: Methods Development Using Ion-Pair Chromatography
with Suppressed Conductivity Detection............................... 0705 TN 46: Determination of Trace Anions in Concentrated
Glycolic Acid.......................................................................... 1057
TN 16: Eluent Preparation for First Generation
Dionex Columns................................................................. 032387 TN 47: Achieving Low Baseline Noise for Anion
Determination by Suppressed Conductivity
TN 19: Gradient Elution in Ion Chromatography: Anion
Using Carbonate Eluents........................................................ 1094
Exchange with Conductivity Detection............................. 032834
TN 48: Determination of Trace Anions in High-Purity Water

TN 20: Analysis of Carbohydrates by High-Performance Anion
by High-Volume Direct Injection with the EG40.................. 1127
Exchange Chromatography with Pulsed Amperometric
Detection (HPAE-PAD)..................................................... 032857 TN 50: Determination of the Amino Acid Content of
Peptides by AAA-Direct........................................................ 1197
TN 21: Optimal Settings for Pulsed Amperometric Detection of
Carbohydrates Using Dionex Pulsed Electrochemical TN 53: Determination of Glycoprotein Monosaccharide
and Amperometric Detectors............................................. 034889 Composition by HPAE-PAD Using On-Line
Electrolytically Generated Eluents......................................... 1405
TN 23: Ion Chromatography of Lanthanide Metals.......... 032889
TN 54: Using Chromeleon Chromatography Management
TN 24: Determination of Chromium by
Software to Comply with 21 CFR Part 11............................. 1302
Ion Chromatography.......................................................... 034350
TN 55: Screening of Sample Matrices and Individual Matrix
TN 25: Determination of Transition Metals in Complex Matri-
Ingredients for Suitability in AAA-Direct............................. 1503
ces by Chelation Ion Chromatography.............................. 034365
TN 57: Automated System Suitability Testing
TN 26: Determination of Cr(VI) in Water, Wastewater, and
with Chromeleon.................................................................... 1716
Solid Waste Extracts . ........................................................ 034398
TN 59: UltiMate 3000 Retention Time Precision.................. 1868
TN 27: Determination of Lanthanide Metals in Digested Rock
Samples by Chelation Ion Chromatography...................... 034664 TN 60: UltiMate 3000—Direct Sample Injection onto a 75-mm
I.D. PepMap 100 (C18) Nanocolumn.................................... 1869
326 www.dionex.com
Books
TN 61: UltiMate 3000 — Preconcentration on a 75-mm i.d. x TN 700: Optimizing the ASI‑100 Autosampler Injection
15 cm PepMap 100 (C18) Nanocolumn................................ 1870 Precision and Linearity Using PrimeSyringe........................ 1646
TN 62: Reducing Carbonate Interference in Anion Determina- TN 701: Sub One-Minute, Nine-Component Gradient HPLC
tions with the Carbonate Removal Device (CRD)................ 1765 Separation for Increased Productivity Using an
Acclaim 120 3-µm C18 Column............................................ 1738
TN 63: Peak Identification and Estimation of Percent Purity
Using HPAE With 3-D Amperometry.................................... 1757 TN 702: Using the Chromeleon Summit x2
Tandem Operation Wizard..................................................... 1740
TN 64: Using the AS Automated Sampler in the Simultaneous,
Sequential, and Concentrate Modes....................................... 1756 TN 703: Using the Chromeleon SmartStart Wizard.............. 1741
TN 65: Using Chromeleon in a Networked Environment..... 1769 TN 704: Fast HPLC Using the Dionex Summit
HPLC System......................................................................... 1739
TN 67: Instrument Control and Data Acquisition
with Chromeleon.................................................................... 1781 TN 705: Automated Enrichment and Analysis of
Phosphopeptides Using Immobilized Metal Affinity and
TN 70: Data Reporting in Chromeleon................................. 1925 RP Chromatography with Column Switching....................... 1763
TN 71: Eluent Preparation for High-Performance Anion-Ex-
change Chromatography with Pulsed Amperometric Books
Detection................................................................................. 1932
The following is available for purchase. Please contact your lo-
TN 72: Reduced Solvent Consumption Mode Allows
cal Dionex representative for pricing.
Continuous Nano LC Operation for One Month................... 2070
Ion Chromatography, Third Edition

ASE Technical Notes by Joachim Weiss (English)............................................... 034996
TN 206: Investigations of Thermal Degradation

During Accelerated Solvent Extraction................................. 0765
TN 207: Investigation of Carryover or Cross-

Contamination in the Dionex ASE 200.................................. 0766
TN 208: Methods Optimization in

Accelerated Solvent Extraction.............................................. 0982
TN 209: Accelerated Solvent Extraction (ASE) Sample Prepara-

tion Techniques for Food and Animal Feed Samples............ 1781

TN 210: Accelerated Solvent Extraction Techniques for In-Line
Selective Removal of Interferences........................................ 1931
Mass Spectrometry Application Briefs

and Technical Notes
Dionex Mass Spectrometry Applications Guide................... 1955
TN 512: MS Detection with the AQA for LC and IC Using

Atmospheric Pressure Ionization Techniques . ..................... 1264
TN 519: Extending the Performance of LC/MS to Nonvolatile

Buffers with the AQA . .......................................................... 1274
Automation Product Technical Notes
TN 67: Instrument Control and Data Acquisition

with Chromeleon.................................................................... 1781
TN 70: Data Reporting in Chromeleon................................. 1925
www.dionex.com 327
Service & Technical Support
From instrument service to validation and
performance testing, we’re here to help
From instrument servicing to validation, Dionex provides the
tools and expertise you need to set up your system, keep it
performing expertly, and pass method validation and audits.
Several Service Plans, Performance Kits, and Validation Kits are
available.
Visit our Support pages on www.dionex.com to find: for the user. Validating the software installation is as simple as
choosing a menu option and printing out the results.
• Answers to Frequently-Asked Questions
To help automate the instrument validation process, OQ/PQ
• Offices
procedures and pass/fail criteria are built into the software.
• Service Contracts Validation “wizards” walk you through the creation of methods
to validate your specific HPLC or IC configuration.
• Service Products
Comprehensive Service Plans Peak Performance Kits

Dionex Peak Performance Kits are designed to meet the pre-
ventative maintenance needs of your Dionex hardware. Each
Superior Service
maintenance kit includes the required spare parts to perform the
When you purchase a Dionex Support Plan, you are investing in recommended annual maintenance on the specified module.
a world-class support organization. Dionex will dedicate the re-
quired resources to ensure that long after purchase, your system Dionex will perform this maintenance for you, or you can
performs the way you need it to. follow the included instructions and perform the maintenance
yourself.
Dionex offers varied levels of comprehensive service plans to
meet the most stringent laboratory requirements for uptime and
maintenance. Contact Information
Service plans vary by region; contact your local office for For service and technical support, contact our Technical Support
details. See “Dionex Locations” in the Ordering Information Department:

section for local contact information.
e-mail: techsupport@dionex.com
phone: 1-800-DIONEX-0 (zero), within U.S. from 8:00am EST
Validation Services to 5:00pm PST
Dionex offers a full range of validation services and kits. or fill out our Technical Support Request Form online:
Trained field service representatives are available to perform
installation, operation, and performance qualification on your http://www1.dionex.com/forms/dnex_tech_support_request.html
HPLC or IC system.
See also “Dionex Locations” in the Ordering Information sec-
Performance validation kits are also available that contain all tion for local contact information to inquire about local service
the tools, worksheets, methods, certificates, and validation tags offerings.
necessary to validate individual system components.
Automating the Validation Process

Dionex data systems, Chromeleon 6 and PeakNet 6, were
designed to make all aspects of validation as easy as possible
328 www.dionex.com
Training
Complete solutions for your analytical needs
Dionex’s ultimate goal is to provide you with an end-to-end
solution to your analytical needs. To meet this goal, we offer a
complete line of services, including a training program to help
you get the most out of your instrumentation and results.
Contact your local Dionex representative to find out about spe-

cific training offerings in your area. (See “Dionex Locations” in
the Ordering Information section.) Training Options
Visit us on the web at:
www1.dionex.com/en-us/training/lp2514.html
Specialized
for details about training offered in your region. Designed specifically to your application and instrumentation
requirements. Assist the chromatographer in maximizing the
capabilities of their system (e.g., IC, ICS, ASE, UltiMate, Sum-
mit). Example courses include:
Course Offerings
An Introduction to the Principles and Practices of HPLC
Instrumentation Accelerated Solvent Extraction (ASE) Training
These courses provide the skills to operate your chromatogra- Carbohydrate Determination by HPAE-PAD
phy system and optimize its analytical and automation capabili- In Search of Resolution: A Practical Approach to Reversed-
ties. Example courses include: phase HPLC Method Development
Ion Chromatography Systems: Instrument-specific courses in Basic Mass Spectrometery, MSQ

basic operation and troubleshooting, and advanced maintenance
Method Development in Ion Chromatography
and troubleshooting
HPLC Systems: Instrument operation, maintenance and trouble-
shooting Customized Training
MSQ Detector Family Do you need a training session customized to your individual

IC Method Development needs? Perhaps you need training geared towards a specific
application or need advanced training on a particular topic.
Analyte-Specific Analysis Whatever your needs, contact the Dionex Training group and
we will put together a course to suit your needs.
Software
Take advantage of the benefits you obtain when participating
On-Site Training
in a Dionex Chromatography Software training course. As you
Do you have a larger group to train? Or perhaps you would like
become more familiar with Dionex Chromatography Software,
to have training on your own instruments. Whatever the reason,
you will appreciate the power and capabilities this software
let us come to you and train you at your facility. Your on-site
provides. Courses include:
training session can include any combination of our standard
courses and/or customized courses to meet individual needs.
Chromeleon Level 1: An Introduction to Chromeleon
Chromeleon Level 2: Next Steps in Chromeleon
Chromeleon Level 3: Chromeleon Advanced Operator Training Web-Based Training
Chromeleon System Administrator Training Interactive training sessions advance your knowledge of
Chromeleon Software. These sessions use live online meeting
technology with desktop sharing to answer your questions and
guide you through solutions.
www.dionex.com 329
Index of Part Numbers
Part Number........................Page 040187 .....................................297 045972 .......................................87 051795 .....................................209
033440 .....................................298 040192 .....................................227 046023 .....................................223 052200 .....................................209
033441 .....................................298 040896 .....................................296 046026 .....................................231 052244 .....................................189
033442 .....................................298 042014 .......................................81 046027 .....................................234 052248 .....................................189
033443 .....................................298 042150 .....................................147 046031 .......................................81 052252 .....................................209
033444 .....................................298 042151 .....................................147 046032 .......................................81 052256 .....................................209
033445 .....................................298 042152 .....................................147 046034 .....................................187 052324 .......................................67
033478 .....................................298 042153 .....................................147 046035 .....................................187 052324 .......................................68
035321 .....................................131 042154 .......................................81 046055 .....................................187 052324 .......................................69
035360 .....................................298 042189 .....................................147 046056 .....................................187 052324 .......................................71
035361 .....................................298 042191 .....................................147 046070 .....................................297 052576 .....................................217
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330 www.dionex.com
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332 www.dionex.com
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163584 .....................................256 5035.9200 ..................................15 5701.2000 ..................................59 5840.0020 ..................................18
163585 .....................................256 5035.9200 ..................................17 5701.2020 ..................................59 5840.0020 ..................................21
163586 .....................................256 5035.9200 ..................................18 5701.2021 ..................................59 5840.0020 ..................................23
163587 .....................................256 5035.9200 ..................................18 5701.2022 ..................................59 5840.0020 ..................................38
163589 .....................................257 5035.9200 ..................................19 5701.2023 ..................................59 6035.0610 ..................................43
163591 .....................................257 5035.9200 ..................................26 5701.2024 ..................................59 6035.0665 ..................................15
163592 .....................................257 5035.9200 ..................................31 5713.0200 ..................................58 6035.0665 ..................................43
163593 .....................................257 5035.9200 ..................................34 5713.6307 ..................................58 6035.0670 ..................................43
163594 .....................................257 5035.9230 ..................................23 5713.6319 ..................................58 6035.1977 ..................................31
163595 .....................................257 5035.9230 ..................................23 5713.6328 ..................................58 6035.1977 ..................................32
163937 .....................................257 5035.9230 ..................................24 5713.6338 ..................................58 6035.1978 ..................................31
334 www.dionex.com
6035.1978 ..................................32 6074.0320 ..................................19 6721.0050 ................................303 6820.1419 ..................................39
6035.1978 ..................................32 6074.0360...................................24 6721.0051 ................................303 6820.1419 ..................................40
6035.1979 ..................................31 6074.0360...................................53 6721.0052 ................................303 6820.1419 ..................................41
6035.1979 ..................................32 6074.0360...................................54 6721.0053 ................................303 6820.1419 ..................................42
6035.1979 ..................................32 6074.0360 ..................................18 6721.0054 ................................303 6820.1419 ..................................43
6035.1979 ..................................33 6074.1110...................................53 6721.0055 ................................303 6820.2415 ..................................18
6035.3000 ..................................30 6074.1110...................................54 6721.0056 ................................303 6820.2415 ..................................21
6035.3000 ..................................31 6074.1110 ..................................51 6721.0067 ................................303 6820.2415 ..................................24
6035.3000 ..................................32 6074.1110 ..................................52 6721.0095 ..................................45 6820.2415 ..................................38
6035.3000 ..................................32 6074.2000 ..................................51 6721.0095 ..................................46 6820.2415 ..................................39
6035.3000 ..................................33 6074.2000 ..................................52 6721.0095 ..................................46 6820.2436 ..................................42
6035.3000 ..................................34 6074.2000 ..................................53 6721.0097 ..................................45 6820.3115 ..................................43
6035.3000 ..................................34 6074.2000 ..................................54 6721.0097 ..................................46 6820.4070 ..................................38
6037.0001 ..................................23 6082.0100...................................21 6721.0097 ..................................46 6820.4070 ..................................39
6037.0001 ................................302 6082.0100 ..................................17 6721.0099 ..................................45 6820.4070 ..................................40
6037.0002 ..................................24 6082.0100 ..................................17 6721.0099 ..................................46 6820.4070 ..................................41
6037.0002 ................................302 6082.0100 ..................................23 6721.0099 ..................................46 6820.4070 ..................................42
6037.0004 ................................302 6082.0100 ..................................51 6721.0100 ................................303 6820.4070 ..................................43
6037.0005 ................................302 6082.0100 ..................................52 6721.0101 ................................303 6820.4079 ..................................38
6037.0006 ................................302 6082.0300...................................21 6721.0102 ................................303 6820.4079 ..................................39
6037.0007 ................................302 6082.0300 ..................................51 6721.0103 ................................303 6820.4079 ..................................40
6037.0008 ................................302 6082.0300 ..................................52 6722.00530 ................................48 6820.4079 ..................................42
6037.0009 ................................302 6510.0004 ..................................27 6722.00530 ................................49 6820.4079 ..................................43
6037.0010 ................................302 6710.1505 ..................................45 6722.00540 ................................48 6820.4084 ..................................38
6037.0014 ................................302 6710.1505 ..................................46 6722.00540 ................................49 6820.4084 ..................................39
6037.0015 ................................302 6710.1505 ..................................46 6722.00550 ................................48 6820.4084 ..................................40
6037.1977 ..................................31 6710.1505 ..................................48 6722.00550 ................................49 6820.4084 ..................................41
6037.1977 ..................................32 6710.1505 ..................................49 6722.0100 ................................302 6820.4084 ..................................42
6037.1978 ..................................31 6713.0022 ..................................58 6722.0101 ................................302 6820.4084 ..................................43
6037.1978 ..................................32 6713.0025 ..................................58 6722.9013 ..................................48 6820.4086 ..................................38
6037.1979 ..................................31 6720.0047 ................................303 6722.9013 ..................................49 6820.4086 ..................................39
6037.1979 ..................................32 6720.0048 ................................303 6722.9023...................................24 6820.4086 ..................................40
6040.5000 ..................................29 6720.0049 ................................303 6722.9023 ..................................23 6820.4086 ..................................41
6040.5150 ..................................21 6720.0050 ................................303 6722.9023 ..................................48 6820.4086 ..................................42
6040.5150 ..................................29 6720.0051 ................................303 6722.9023 ..................................49 6820.4086 ..................................43
6074.0200...................................53 6720.0052 ................................303 6722.9035 ..................................48 6820.4087 ..................................38

6074.0200...................................54 6720.0053 ................................303 6722.9035 ..................................49 6820.4087 ..................................39
6074.0200 ..................................18 6720.0054 ................................303 6723.9013 ..................................48 6820.4087 ..................................40
6074.0200 ..................................23 6720.0055 ................................303 6723.9013 ..................................49 6820.4087 ..................................41
6074.0250...................................53 6720.0056 ................................303 6723.9023 ..................................23 6820.4087 ..................................42
6074.0250...................................54 6720.0067 ................................303 6723.9023 ..................................48 6820.4087 ..................................43
6074.0250 ..................................15 6720.0095 ..................................45 6723.9023 ..................................49 6820.4090 ..................................38
6074.0250 ..................................15 6720.0095 ..................................46 6730.0001 ..................................48 6820.4090 ..................................39
6074.0270...................................53 6720.0095 ..................................46 6730.0002 ..................................48 6820.4090 ..................................40
6074.0270...................................54 6720.0097 ..................................45 6730.0003 ..................................23 6820.4090 ..................................41
6074.0270 ..................................19 6720.0097 ..................................46 6730.0003 ..................................48 6820.4090 ..................................42
6074.0270 ..................................24 6720.0097 ..................................46 6730.0004 ..................................23 6820.4090 ..................................43
6074.0280...................................53 6720.0099 ..................................45 6730.0004 ..................................48 6820.4091 ..................................38
6074.0280...................................54 6720.0099 ..................................46 6730.0006 ..................................48 6820.4091 ..................................39
6074.0290...................................53 6720.0099 ..................................46 6730.0008 ..................................48 6820.4091 ..................................40
6074.0290...................................54 6720.0100 ................................303 6820.0017 ..................................43 6820.4091 ..................................41
6074.0300...................................53 6720.0101 ................................303 6820.0018 ..................................43 6820.4091 ..................................42
6074.0300...................................54 6720.0102 ................................303 6820.0020 ..................................43 6820.4091 ..................................43
6074.0305 ..................................53 6720.0103 ................................303 6820.0031 ..................................43 6821.0017 ..................................43
6074.0305 ..................................54 6721.0047 ................................303 6820.0050 ................................303 6821.0018 ..................................43
6074.0320...................................53 6721.0048 ................................303 6820.0051 ..................................57 6821.0020 ..................................43
6074.0320...................................54 6721.0049 ................................303 6820.1419 ..................................38 6821.0031 ..................................43
www.dionex.com 335
6821.0050 ..................................24
6821.0050 ................................303
6821.0051 ..................................57
6821.3115 ..................................40
6821.3115 ..................................43
6822.0002 ..................................43
6822.0005 ..................................41
6822.2432 ..................................38
6822.2432 ..................................39
6822.2433 ..................................38
6822.2433 ..................................39
6823.0016 ..................................40
6823.0017 ..................................40
6823.0018 ..................................40
6830.2442 ..................................41
706.200.75021............................55
706.228.3384391 . .....................55
706.228.34197.93 . ....................55
80024 .......................................106
SP2089 ....................................279
SP2091 ....................................262
SP2667 ....................................279
SP2866 ....................................273
SP3229 ....................................262
SP4008 ....................................262
SP4016 ....................................262
SP4216 ....................................275
SP4354 ....................................131
SP4356 ....................................131
SP5482 ....................................241
SP5488 ....................................268
SP5490 ....................................268
SP5512 ....................................241
SP5513 ....................................241
SP5522 ....................................241
SP5578 ....................................275
SP5594 ....................................243
SP5598 ....................................243
SP5678 ....................................268
SP5680 ....................................268
SP5727 ....................................241
SP5728 ....................................241
SP5729 ....................................243
SP5730 ....................................243
SP5731 ....................................243
SP5732 ....................................243
SP5829 ....................................241
SP6731 ....................................263
SP6734 ....................................263
336 www.dionex.com
Contact your local sales office for ordering and
additional information
Order terms and conditions vary by region. Please contact your
local Dionex representative for more information.
Dionex Locations
Corporate Headquarters India

Dionex India Pvt. Ltd
Location R-610, Rabale, TTC MIDC
Navi Mumbai 400 701 India
1228 Titan Way
Phone: (91) 22 2764 2735
Sunnyvale, CA 94085
Fax: (91) 22 2764 2733
U.S.A.
Japan
Mailing Address Nippon Dionex K.K.
500 Mercury Drive DNX Shin-Osaka Bldg.
P.O. Box 3603 6-3-14 Nishi-Nakajima
Sunnyvale, CA 94088-3603 Yodogawa-ku
U.S.A. Osaka 532-0011 Japan
Phone: (81) 6 6885 1213
Telephone and Fax Fax: (81) 6 6885 1215
Fax: (408) 730-9403 Korea
Tel: (408) 737-0700 Dionex Korea Ltd.
Tel (orders or Return Goods Authorization): Ace High-Tech City, 3-601
1-800-DIONEX-0 (6:00 a.m. to 5:00 p.m. PST) Mullae-dong 3-ga 54-66
Yeongdeungpo-gu
Seoul, 150-972 Korea
Worldwide Offices and Subsidiaries Phone: (82) 2 2653 2580
Fax: (82) 2 2653 2508

Asia Pacific
Singapore
Australia Dionex Singapore Pte. Ltd.
Dionex Pty Ltd 14 Little Road #05-02
Unit 31, 2 Chaplin Drive Tropical Industrial Building
Lane Cove, NSW 2066 Australia 536987 Singapore
Phone: (61) 2 9420 5233 Phone: (65) 6289 1190
Fax: (61) 2 9420 5244 Fax: (65) 6289 2230
China Taiwan
Dionex China Ltd. Dionex Taiwan Ltd.
Room 2001, Level 20 1F., No.72 Jhouzih St.
Metroplaza Tower 2 Neihu District
223 Hing Fong Road Taipei City, 11493 Taiwan
Kwai Fong, N.T. Hong Kong China Phone: (886) 2 8751 6655
Phone: (852) 2428 3282 Fax: (886) 2 8751 5353
Fax: (852) 2428 7898
www.dionex.com 337
Europe
Austria Sweden
Dionex Austria GmbH Dionex Sweden AB
Laxenburger Strasse 220 Månskärsvägen 9, 4 tr
1230 Wien Austria S-141 75 Kungens Kurva
Phone: (43) 1 616 51 25 Phone: (46) 8 473 3380
Fax: (43) 1 616 51 25 55 Fax: (46) 8 180 717
Benelux Switzerland
Dionex Benelux B.V. Dionex (Switzerland) AG
Abberdaan 114 Solothurnerstr. 259
1046 AA Amsterdam The Netherlands 4600 Olten Switzerland
Phone: (31) 20 683 9768 Phone: (41) 62 205 99 66
Fax: (31) 20 685 3452 Fax: (41) 62 205 99 60
Phone: (32) 3 353 42 94
Fax: (32) 3 353 42 93 United Kingdom
Dionex (UK) Ltd.
Denmark 4 Albany Court
Dionex Denmark A/S Camberley
Stamholmen 193 Surrey, GU16 7QL United Kingdom
2650 Hvidovre Denmark Phone: (44) 1276 691722
Phone: (45) 36 36 90 90 Fax: (44) 1276 691837
Fax: (45) 36 36 90 99
The Americas
France
Dionex S.A.
Brazil
164-166 Avenue Joseph Kessel
Dionex Brasil
78960 Voisins Le Bretonneux France
Instrumentos Cientificos Ltda.
Phone: (33) 1 39 30 01 10
Av Comendador Alberto Bonfiglioli 669
Fax: (33) 1 39 30 01 12
CEP: 05593-001 Sao Paulo Brasil
Phone: (55) 11 3731 5140
Germany
Fax: (55) 11 3213 9530
Dionex GmbH
Am Wörtzgarten 10
Canada
65510 Idstein Germany
Dionex Canada Ltd.
Phone: (49) 6126 991 0
1540 Cornwall Road, Suite 204
Fax: (49) 6126 991 272

Oakville, ON
L6J 7W5 Canada
Ireland
Phone: (905) 844 9650
Dionex Ireland Ltd.
Fax: (905) 844 6134
Suite 3C
Plato Business Park
United States
Damastown
Dionex North American Sales and Service Headquarters and
Dublin 15 Ireland
Centers of Excellence
Phone: (353) 1 644 0064
3000 Lakeside Drive, Suite 116N
Fax: (353) 1 885 1673
Bannockburn, IL 60015 USA
Phone: (847) 295 7500
Italy
Fax: (847) 283 0722
Dionex S.p.A.
Via XXV Aprile 6
20097 San Donato Milanese (MI) Italy
Phone: (39) 02 51 62 1267
Fax: (39) 02 51 62 8238
338 www.dionex.com
www.dionex.com
Dionex products are designed,

developed, and manufactured
under an ISO 9001 Quality System.
Analyst is a registered trademark of Applied Biosystems.

Hystar is a trademark of Bruker Biosystems.
FastLoc, MSQ Plus, and Xcalibur are trademarks of Thermo Electron Corporation.
All other trademarks and registered trademarks are the property of Dionex Corporation.
LPN 2002-02 40M 08/08 Printed in U.S.A.

© 2008 Dionex Corporation
Passion. Power. Productivity.

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Contents

Liquid Chromatography Instruments 5 IC & RFIC Column Compartments.................................... 83

UltiMate 3000 LC Systems................................... 13 Mass Spectrometry 95

IC & RFIC Systems................................................ 63

IC & RFIC Modules................................................ 73

IonPac AS5......................................................................... 198

LC Columns 117 Cation-Exchange Colums................................... 201

Specialty LC Columns......................................... 133 Transition Metal Columns.................................. 215

Supplies & Accessories...................................... 289

Indices & Appendices 305

Column Selection Guide.................................... 307

Column Specifications........................................ 311

Liquid Chromatography Instruments

LC Injectors & Autosamplers............................... 37

software features to provide turnkey productivity suites for your

Learn more about LCi Solutions on www.dionex.com under LC

Parallel Analyses • No revalidation of methods required

• Run two different or identical LC applications in parallel

Liquid Chromatography Instruments

Figure 4. Flow scheme for automated on-line SPE-LC.

Chromeleon Chromatography Management Software provides

WVL: 210 nm WVL: 235 nm

Chromeleon Chromatography Management Software provides

Liquid Chromatography Instruments

Figure 8. Flow scheme for proteomics parallel LC.

The parallel LC setup allows simultaneous peptide separation

sample injection and separation in the first dimension, collec-

2-D LC Salt Steps

Figure 11. Flow scheme for comprehensive 2-D LC.

The UltiMate 3000 Proteomics MDLC system for 2-D LC

Liquid Chromatography Instruments

• Speed up your analytical LC application by up to a factor

applications at flow rates up to 5 mL/min and backpressures up

Zone 1 Zone 2 Zone 3 Zone 4

Liquid Chromatography Instruments

UltiMate 3000 Basic Automated System

Product Data Sheets

ACC-3000 Autosampler Column Compartment

Basic Manual System

• SmartFlow pulsation-free flows even at high flow rates and

Note: For system specifications, please refer to the LC

Liquid Chromatography Instruments

Hardware Note: For system specifications, please refer to the LC

The UltiMate 3000 Basic Automated System provides rugged,

• Excellent retention time and gradient precision due to

Binary Analytical System

The UltiMate 3000 Binary Analytical system has been carefully

• SmartFlow technology for precise and accurate flow rates

Liquid Chromatography Instruments

The following modules and accessories represent a typical

Note: For system specifications, please refer to the

The following modules and accessories represent a typical

Liquid Chromatography Instruments

Accessories Note: For system specifications, please refer to the

• UltiFlow technology for precise and accurate flow rates

The UltiMate 3000 Rapid Separation LC (RSLC) system

• SmartFlow pulsation-free flows at flow rates up to 5 mL/

Area RSD: 0.12%

Liquid Chromatography Instruments

Area RSD: 0.23%

Excellent retention time and

Product Data Sheets

UltiMate 3000 Rapid Separation LC (RSLC) System Data Sheet