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  • Julie Bui - Introduction To The Light Microscope

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    The document introduces the parts of a light microscope and provides instructions on how to use it. It lists the main components of a microscope, including the binocular eyepiece, objective lenses, stage, light source, and focus controls. It then outlines the steps to locate and focus on a specimen, adjust the optics, and observe details under high power. Finally, it discusses calibrating the microscope to calculate fields of view and estimate sizes of objects.

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    The document introduces the parts of a light microscope and provides instructions on how to use it. It lists the main components of a microscope, including the binocular eyepiece, objective lenses, stage, light source, and focus controls. It then outlines the steps to locate and focus on a specimen, adjust the optics, and observe details under high power. Finally, it discusses calibrating the microscope to calculate fields of view and estimate sizes of objects.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    Download as pdf or txt
    666 views2 pages

    Julie Bui - Introduction To The Light Microscope

    Uploaded by

    api-522650514
    The document introduces the parts of a light microscope and provides instructions on how to use it. It lists the main components of a microscope, including the binocular eyepiece, objective lenses, stage, light source, and focus controls. It then outlines the steps to locate and focus on a specimen, adjust the optics, and observe details under high power. Finally, it discusses calibrating the microscope to calculate fields of view and estimate sizes of objects.

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    Julie Bui

    Mr. Mantoani
    HSB 1- Period 1
    8 September 2020
    Introduction to the Light Microscope
    Virtual Urchin:
    Microscope Tutorial​-
    1. Identify the different parts of the microscope
    - Binocular eyepiece (may have a monocular eyepiece)
    - Objective lenses (usually has three objectives)
    - Stage (the platform you use to perform your slides)
    - Light source (main/actual source of light)
    - Course focus control
    - Fine focus control
    - Diaphragm
    - Dimmer Switch
    - Stage Control Adjustments

    2. Learn how to locate and focus on an object (specimen) in the microscope


    3. Adjust the optics to obtain a clear image of the specimen
    4. Observe the details of a specimen under high power
    - #2-4 completed virtually

    Microscope Measurement​:
    1. Calibrate your microscope
    - That is when you calculate the different diameters of the fields of view at each
    magnification
    2. Use your microscope calibration to estimate the size of objects
    Virtual Microscope​: the document below

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