How Can You

Reduce the Time to

Guidelines
27 Fast Effective Antibiotic
Treatment in Patients
A. Surviving Sepsis Campaign: International Guidelines for Management
of Sepsis and Septic Shock: 2016. https://www.ncbi.nlm.nih.gov/ TARGETS Accurate
pubmed/28098591.
B. Implementing an Antibiotic Stewardship Program: Guidelines by the
Infectious Diseases Society of America and the Society for Healthcare
Epidemiology of America. Clin Infect Dis. 2016 May 15;62(10):e51-77.
1hr Comprehensive
with Bloodstream
doi: 10.1093/cid/ciw118. Epub 2016 Apr 13. GRAM-NEGATIVE
BACTERIA
Acinetobacter baumannii
GRAM-POSITIVE
BACTERIA
Enterococcus
YEAST
Candida albicans
Candida glabrata
Infections?
Enterobacteriaceae Listeria monocytogenes Candida krusei
Enterobacter cloacae complex Staphylococcus Candida parapsilosis

“ Addition of the BioFire BCID Panel to an

established ASP (Antibiotic Stewardship
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Staphylococcus aureus
Streptococcus
Streptococcus agalactiae
Candida tropicalis

ANTIMICROBIAL
Don’t Guess. Know.
Program) shortened the time to effective Proteus Streptococcus pneumoniae RESISTANCE
therapy and further improved antimicrobial use Serratia marcescens Streptococcus pyogenes GENES
compared to ASP alone, even in a setting of low Haemophilus influenzae mecA – methicillin resistance

”
Neisseria meningitidis vanA/B – vancomycin resistance
antimicrobial resistance rates. Pseudomonas aeruginosa KPC – carbapenem resistance
(MacVane & Nolte, J Clin Microbiol 20163)

Pathogens Under Surveillance15

References •
•
Group A Streptococcus
Haemophilus influenzae
•
•
Listeria monocytogenes
Neisseria meningitidis
1. Buehler SS et al. Effectiveness of Practices to Increase Timeliness of Providing Targeted Therapy for Inpatients
with Bloodstream Infections: a Laboratory Medicine Best Practices Systematic Review and Meta-analysis. Clin • Streptococcus pnuemoniae
Microbiol Rev 2016; 29:59-103.
2. Kumar A et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical
determinant of survival in human septic shock. Crit Care Med 2006; 34:1589-1596.
3. MacVane SH & Nolte FS. Benefits of Adding a Rapid PCR-Based Blood Culture Identification Panel to an
Established Antimicrobial Stewardship Program. J Clin Microbiol 2016; 54:2455-2463.
Where to Order This Test?
4. Fiori B et al. Optimized Use of the MALDI BioTyper System and the FilmArray BCID Panel for Direct Identification
of Microbial Pathogens from Positive Blood Cultures. J Clin Microbiol 2016; 54:576-584.
5. Paolucci M et al. Comparison between MALDI-TOF MS and FilmArray Blood Culture Identification panel for rapid
identification of yeast from positive blood culture. J Microbiol Methods 2014;104:92-93.
6. Messacar K et al. Clinical Impact and Provider Acceptability of Real-Time Antimicrobial Stewardship Decision
Support for Rapid Diagnostics in Children With Positive Blood Culture Results. J Pediatric Infect Dis Soc 2016:
piw047.
7. Ray ST et al. Rapid Identification of Microorganisms by FilmArray Blood Culture Identification Panel Improves
Clinical Management in Children. Pediatr Infect Dis J 2016; 35:e134-138.

BFDX-MKT-0342-01
8. Banerjee R et al. Randomized Trial of Rapid Multiplex Polymerase Chain Reaction-Based Blood Culture
Identification and Susceptibility Testing. Clin Infect Dis 2015; 61:1071-1080.
9. MacVane SH et al. Impact of a rapid multiplex polymerase chain reaction blood culture identification technology
on outcomes in patients with vancomycin-resistant Enterococcal bacteremia. Infect Dis (London) 2016; 48:732-
737.
10. Pardo J et al. Clinical and economic impact of antimicrobial stewardship interventions with the FilmArray blood
culture identification panel. Diagn Microbiol Infect Dis 2016; 84:159-164.
The information in this booklet is given as a guideline only and is not intended to be
11. Data on file, BioFire Diagnostics. The stated performance is the overall aggregate performance of the prospective exhaustive. It in no way binds bioMérieux or BioFire Diagnostics, LLC to the diagnosis
clinical study data presented in the IFU. Sensitivity and specificity data only relate to pathogen detection. established or the treatment prescribed by the physician. If you have any questions
12. Altun O et al. Clinical Evaluation of the FilmArray Blood Culture Identification Panel in Identification of Bacteria please contact your bioMérieux representative. BioFire is wholly owned by bioMérieux.
and Yeast from Positive Blood Culture Bottles. J Clin Microbiol 2013;51:4130-4136.
13. Kim et al. Patient Outcomes Associated with Implementation of a Multiplex Assay for the Identification of bioMérieux S.A. Manufactured by:
Staphylococcus aureus Bloodstream Infections 69280 Marcy I’Etoile BioFire Diagnostics, LLC
14. Timbrook et al. The Effect of Molecular Rapid Diagnostic Testing on Clinical Outcomes in Bloodstream Infections: France 515 Colorow Drive
A Systematic Review & Meta-analysis. Clinical Infectious Diseases Advance Access published September 26, Tel.: +33 (0) 4 78 87 20 00 Salt Lake City, UT 84108
2016and Yeast from Positive Blood Culture Bottles. J Clin Microbiol 2013;51:4130-4136. Fax: +33 (0) 4 78 87 20 90 USA
15. https://wwwn.cdc.gov/nndss/conditions/. www.biomerieux.com www.biofiredx.com
What’s the Problem? Early Pathogen Identification
While standard of care has improved over recent years with is Essential.
MALDI-TOF, it still requires culture isolated organisms and
does not rule out the possibility of polymicrobial infections, For every hour of delay in initiation of effective antimicrobial
which account for up to 12.3% of positive blood culture.4 treatment following onset of septic shock, patient survival
declines 7.6%.²
The BioFire® FilmArray® Blood Culture Identification
(BCID) Panel provides microbiologists and antimicrobial
stewardship programs (ASPs) with rapid microbial 85% 8%
Patient Patient
identification, along with resistance marker detection. This Survival Survival
Antimicrobial Stewardship.
Antimicrobial stewardship programs (ASPs) optimize
antimicrobial use to achieve the best clinical outcomes.
The BioFire BCID Panel rapid pathogen identification, in
combination with locally derived treatment guidelines set by
an ASP, can result in appropriate antimicrobial interventions.7
Combined with appropriate antimicrobial stewardship,
the BioFire BCID Panel:
HOSPITAL

information can enable the development of hospital-centric Rate Rate

therapeutic guidelines that may help clinicians quickly adopt
a more targeted therapy.
0 36
Diagnostic Challenges Decreases time to
effective therapy,8,9
Lessens
unnecessary
Improves time
to antimicrobial
Can reduce
hospital
Hours Hours
In a Typical Case. which can improve
patient survival.2,14
antibiotic use.3,7,8,10 de-escalation.3,8,13 costs.3,5,9,10

Stephen, a 64-year-old clerk with diabetes, presented to the The BioFire BCID Panel identifies 24 commonly found
ED on Saturday at 4 p.m. with a 2-day history of fever and gram-positive bacteria, gram-negative bacteria, and yeast
fatigue. Stephen’s chest X-ray was normal, the auscultation pathogens, and 3 antibiotic resistance genes associated Proven Effect of Antimicrobial
revealed pulmonary crackling rales, the respiratory rate was
22 bpm, and procalcitonin was 1.5 ng/ml.
with bloodstream infections. All in about an hour. Stewardship and the BioFire
BCID Panel.
Stephen was admitted to the hospital, and an empirical Who Should Get Tested?
antibiotic therapy (3rd generation cephalosporin, Prospective Timeline in Hours
aminoglycoside, and vancomycin) was initiated at 7 p.m. Initial empiric antimicrobial therapy is recommended for Randomized Post Gram Stain Result
Sunday morning at 5 a.m., Stephen was non-responsive patients with suspicion of a bloodstream infection or sepsis Study Arms 0 12 24 36
and had developed signs of multi-organ failure. The serum until organism identification and antimicrobial susceptibility
lactate level was 4 mmol/L and Stephen was put on data becomes available. A,3 Traditional Methods
vasopressors. The blood cultures turned positive at 7 a.m.
The BioFire BCID Panel reliably identifies bacteria and BCID
It appears that the empiric antibiotic therapy was not Candida spp. in positive blood cultures from adult and
effective. Rapid identification of the etiologic agent is now pediatric patients with monomicrobial or polymicrobial BCID+Stewardship
imperative. bloodstream infections.
Organism Identification Antimicrobial Escalation Antimicrobial De-Escalation
Syndromic Testing. Adults Children
BioFire’s syndromic testing allows clinicians to quickly
identify infectious agents that produce similar symptoms
Overall Performance of
in patients. BioFire’s innovative PCR technology provides the BioFire BCID Panel.11
answers in a clinically actionable timeframe. Sample Type.
200 µL positive blood culture media.
97.5% Sensitivity 99.8% Specificity

One One Takes about Multiple results Improves patient

sample comprehensive an hour for in one report management
test results