MEDICAL LABORATORY SCIENCE PROGRAM

PRINCIPLES OF
MEDICAL LABORATORY SCIENCE 2

Name__________________________________ Points Earned:_________________________

Section ______________ Group _____________ Date ________________________________

LEARNING ACTIVITY NO. 6

PROPER DONNING AND REMOVING OF PPEs

Specific Learning Objectives:

a. Explain the importance of wearing PPEs such as gloves, mask, shoes and laboratory
gowns

b. Explain the importance of proper donning and removing of PPEs

c. Demonstrate proper donning and removing of PPEs at the different areas of the hospital
including laboratory, patient’s bedside, operating room, and intensive care unit.

PERSONAL PROTECTIVE EQUIPMENT

The Personal Protective Equipment (PPE) includes the gloves, masks, shoes, and
laboratory gowns. It may also include goggles and face shields. These PPEs are used
to protect the laboratory scientist/medical technologist from infectious material.

Masks

The masks prevent the transmission of infectious agents through the air. This should
always be worn by the medical technologists especially when entering the respiratory
isolation room. It should cover both the nose and mouth, and worn only once then
discarded in an appropriate container. Masks are no longer effective once they become
moist from breathing.

Gloves

Gloves are worn due to the following reasons:

1. It prevents the medical technologists from transmitting their own microbiota to the
patient,

2. It prevents transmission of microorganisms from one patient to another; and

3. It prevents the laboratory scientists or medical technologists from being infected by

organisms from the patient.

Laboratory Gowns

Laboratory gowns are worn when soiling of clothes is possible while working in the
laboratory or extracting blood from patients. It should be fluid-resistant to prevent any
blood or body fluids from soaking through and getting on the medical technologist.
Hence, wearing of laboratory gowns prevents transmission of microorganisms from the
patients and environment to the medical technologist. Ideally, it should be used only
once then disposed of in the appropriate receptacle.
 LEARNING ACTIVITY NO. 6A

PROPER DONNING OF PPEs

Principle:

Proper donning and removing of PPEs prevent the transfer of organisms from a source
to a susceptible host.

Materials:

 Biohazard waste container

 Disposable cap

 Disposable gloves (sterile or unsterile)

 Disposable mask

 Laboratory gown (preferably disposable)

 Laboratory shoes

Procedure:

1. Remove all accessories and put away all gadgets. Store them inside the bag or
locker.

2. Remove street shoes and wear laboratory shoes.

3. Wear the mask by placing the top of the mask over the bridge of the nose and pinch
the metal strip to fit the nose. If you are using a mask with strings, tie the top strings
of the mask so the strings are positioned above the ears then tie the lower strings
behind the neck.

4. If necessary, apply the cap to cover hair and ears. Pull long hair up under the cap.

5. Place the gown in front of your body and place your arms through the sleeves of the
gown. If your are wearing a gown with strings, tie the top strings on the gown behind
the neck then tie the lower strings of the gown behind the back.

6. Make sure to button the gown properly.

7. Put on gloves.
 MEDICAL LABORATORY SCIENCE PROGRAM

PRINCIPLES OF
MEDICAL LABORATORY SCIENCE 2

Name________________________________ Points Earned:_________________________

Section ______________ Group_____________ Date ________________________________

LEARNING ACTIVITY NO. 6A

PROPER DONNING OF PPEs

I. STUDENT LEARNING OUTCOME EVALUATION (GROUP WORK - Maximum 50

points)

1. Create a video presentation

2. Choose a representative of your group to perform proper donning of PPEs
3. Submit in a Compact Disc or USB (as prescribed by the instructor)

0 = needs improvement: did not perform

1 = fair: performed below the standard
3 = very good: performed within the standard
5 = excellent: performed beyond the standard

0 1 3 5
GROUP PERFORMANCE
Procedure

1. All accessories were removed and all gadgets were

placed inside the bag or locker.
2. Street shoes were removed and laboratory shoes
were worn properly.
3. Mask was worn properly by placing the top of the
mask over the bridge of the nose and pinching metal
strip to fit the nose.
4. Cap was worn to cover hair and ears. Long hair was
pulled up.
5. Laboratory gown was placed in front of the body and
arms were placed through the sleeves of the gown.
 6. Laboratory gown was buttoned properly
7. Gloves were worn properly.
Mastery
a. The participant was able to show mastery of proper
donning of PPEs
Creativeness

a. The video portrays proper donning of PPEs in actual

laboratory-setting and delivered creatively
Punctuality

a. Video was submitted on time, as deadline prescribed

by the instructor.

COMMENTS:

________________________________________________________________________
_
________________________________________________________________________
_
________________________________________________________________________
_

MEDICAL LABORATORY SCIENCE PROGRAM

PRINCIPLES OF
MEDICAL LABORATORY SCIENCE 2

Name__________________________________Points Earned:_________________________
Section ______________ Group_____________ Date ________________________________

LEARNING ACTIVITY NO. 6A

PROPER DONNING OF PPEs

II. STUDENT LEARNING OUTCOME EVALUATION (RETURN DEMONSTRATION-

Maximum 50 points)

1. Perform proper donning of PPEs within the allotted time prescribed by the instructor

1 = needs improvement: did not perform

1 = fair: performed below the standard
3 = very good: performed within the standard
5 = excellent: performed beyond the standard

0 1 3 5
INDIVIDUAL PERFORMANCE
Preparation
a. The student prepared all materials required in the
procedure
Procedure

1. All accessories were removed and all gadgets were

placed inside the bag or locker.
2. Street shoes were removed and laboratory shoes
 were worn properly.
3. Mask was worn properly by placing the top of the
mask over the bridge of the nose and pinching metal
strip to fit the nose.
4. Cap was worn to cover hair and ears. Long hair was
pulled up.
5. Laboratory gown was placed in front of the body and
arms were placed through the sleeves of the gown.
6. Laboratory gown was buttoned properly
7. Gloves were worn properly.
Mastery

a. The participant was able to show mastery and

spontaneity of performing the procedure
Punctuality

a. The student was able to finish the procedure within

the allotted time

COMMENTS:

________________________________________________________________________
_
________________________________________________________________________
_
________________________________________________________________________
_

MEDICAL LABORATORY SCIENCE PROGRAM

PRINCIPLES OF
MEDICAL LABORATORY SCIENCE 2

Name__________________________________Points Earned:_________________________
Section ______________ Group_____________ Date ________________________________

LEARNING ACTIVITY NO. 6A

PROPER DONNING OF PPEs

III. STUDENT LEARNING OUTCOME EVALUATION (ESSAY- Maximum 12 points)

Rubrics for Essay

Score Content Organization Development Use of

language
4 Answer is Clear sense of Develops each Uses technical or
appropriate to order. Begins point with may scientific
the question. with a thesis or specific details. terminology
Content is topic sentence. Answers appropriately
factually correct. Supporting question and correctly. No
points are completely. major
 presented in a grammatical or
logical spelling errors.
progression.
3 Answer is May lack a Each point is Accurate word
appropriate to thesis sentence, supported with choice. No more
the question. but points are some details and than 2 major
Content may presented in a evidence. All errors and a few
have one or two logical important points minor errors.
factual errors. progression. included.
2 Content relates Logic of Sparse details or Ordinary word
peripherally to argument is evidence. choice; use of
the question; minimally Question only scientific
contains perceivable. partially terminology
significant Points presented answered. avoided. Some
factual errors. in a seemingly serious errors
random fashion, (but they don’t
but all support impair
argument. communication).
1 Content Lacks clear Statements are Limited
unrelated to organizational unsupported by vocabulary;
question. plan. Reader is any detail or errors impair
confused. explanation. communication.
Repetitious,
incoherent,
illogical
development.

LEARNING ACTIVITY NO. 6B

PROPER REMOVAL OF CONTAMINATED GLOVES

Principles:

Gloves after usage should be removed properly to avoid contamination of the laboratory
scientist or medical technologist. This will also decrease the transfer of organisms from
a source to susceptible host.

Materials:

 Biohazard waste container

 Gloves

Procedure:

1. Place hands with gloves in front and away from the body.

2. Grasp the palm of the gloves on the left hand, and pull it down to turn it inside out.
Do not touch the bare skin with the contaminated gloves.

3. The right hand still having the glove on should then hold the inverted and removed
glove.
 4. Contain the inverted glove completely in the gloved hand.

5. Insert two fingers of the ungloved hand under the cuff of the glove on the other
hand.

6. Pull down the glove to turn it inside out.

7. Contain the other glove inside the inverted glove.

8. Dispose the gloves into a biohazard container.

9. Wash hands with running water.

Answer the following questions:

1. Why do you need to grasp the palm of the used glove in step 2?

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 2. Why do you need to insert fingers under the cuff of the other glove?

________________________________________________________________________
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________________________________________________________________________
_

3. Why is there a need to wash hands with running water after removal of gloves?

________________________________________________________________________
________________________________________________________________________
________________________________________________________________________
________________________________________________________________________
________________________________________________________________________
________________________________________________________________________
______

MEDICAL LABORATORY SCIENCE PROGRAM

PRINCIPLES OF
MEDICAL LABORATORY SCIENCE 2

Name__________________________________ Points
Earned:_________________________
Section ______________ Group _____________ Date
________________________________

LEARNING ACTIVITY NO. 6B

PROPER REMOVAL OF CONTAMINATED GLOVES

IV. STUDENT LEARNING OUTCOME EVALUATION (GROUP WORK - Maximum 60

points)

1. Create a video presentation

2. Choose a representative of your group to perform proper removal of
contaminated gloves
3. Submit in a Compact Disc or USB (as prescribed by the instructor)

0 = needs improvement: did not perform

1 = fair: performed below the standard
3 = very good: performed within the standard
5 = excellent: performed beyond the standard

0 1 3 5
GROUP PERFORMANCE
Procedure

1. Place hands with gloves in front and away from the

body.
2. Grasp the palm of the gloves on the left hand, and pull
it down to turn it inside out. Do not touch the bare skin
with the contaminated gloves.
3. The right hand still having the glove on should then
hold the inverted and removed glove.
4. Contain the inverted glove completely in the gloved
hand.
5. Insert two fingers of the ungloved hand under the cuff
of the glove on the other hand
6. Pull down the glove to turn it inside out.
7. Contain the other glove inside the inverted glove.
8. Dispose the gloves into a biohazard container.
9. Wash hands with running water.
Mastery
a. The participant was able to show mastery of proper
removal of contaminated gloves
Creativeness

b. The video portrays proper removal of contaminated

gloves in a unique laboratory-setting
Punctuality

b. Video was submitted on time, as deadline prescribed

by the instructor.

COMMENTS:

________________________________________________________________________
_
________________________________________________________________________
_
 ________________________________________________________________________
_
MEDICAL LABORATORY SCIENCE PROGRAM

PRINCIPLES OF
MEDICAL LABORATORY SCIENCE 2

Name__________________________________ Points
Earned:_________________________
Section ______________ Group _____________ Date
________________________________

LEARNING ACTIVITY NO. 6B

PROPER REMOVAL OF CONTAMINATED GLOVES

V. STUDENT LEARNING OUTCOME EVALUATION (RETURN DEMONSTRATION -

Maximum 60 points)

1. Perform proper removal of contaminated gloves within the allotted time

0 = needs improvement: did not perform

1 = fair: performed below the standard
3 = very good: performed within the standard
5 = excellent: performed beyond the standard

0 1 3 5
INDIVIDUAL PERFORMANCE
Preparation
a. The student prepared all necessary materials for the
procedure
Procedure

1. Place hands with gloves in front and away from the

body.
2. Grasp the palm of the gloves on the left hand, and pull
it down to turn it inside out. Do not touch the bare skin
with the contaminated gloves.
3. The right hand still having the glove on should then
hold the inverted and removed glove.
4. Contain the inverted glove completely in the gloved
hand.
5. Insert two fingers of the ungloved hand under the cuff
of the glove on the other hand
6. Pull down the glove to turn it inside out.
7. Contain the other glove inside the inverted glove.
8. Dispose the gloves into a biohazard container.
9. Wash hands with running water.
Mastery
b. The participant was able to show mastery and
spontaneity of proper removal of contaminated gloves
Punctuality

c. The student performed the procedure within the

allotted time

COMMENTS:
________________________________________________________________________
_
________________________________________________________________________
_
________________________________________________________________________
_

LEARNING ACTIVITY NO. 6C

PROPER REMOVAL OF PPEs

Principle:

Proper removal of PPEs prevents the transfer of organisms from a source to a

susceptible host.

Materials:

• Biohazard waste container

• Disposable cap
• Disposable gloves (sterile or unsterile)
• Disposable mask
• Laboratory gown (preferably disposable)
• Laboratory shoes

Procedure:

1. Remove contaminated gloves properly and discard it into the biohazard

container.

2. Wash hands properly.

3. Remove the mask. If you are using a mask with strings, untie the top ties first
and then the bottom ties. Hold the mask by the ties and drop it into a biohazard
waste container.

4. Remove the laboratory gown. Slip the fingers of one hand inside the cuff of the
gown and pull the gown over the hand.

5. Using the hand covered by the gown, pull the gown down over the other hand.

6. Pull the gown off your arms. Hold the gown away from you and roll it into a ball
with the contaminated side on the inside of the ball.

7. Dispose the gown into the biohazard container or its appropriate receptacle.

8. Remove the cap.

9. Remove the laboratory shoes.

 MEDICAL LABORATORY SCIENCE PROGRAM

PRINCIPLES OF
MEDICAL LABORATORY SCIENCE 2

Name__________________________________ Points Earned:_________________________

Section ______________ Group _____________ Date ________________________________

LEARNING ACTIVITY NO. 6C

PROPER REMOVAL OF PPEs

I. STUDENT LEARNING OUTCOME EVALUATION (GROUP WORK - Maximum 60

points)

1. Create a video presentation

2. Choose a representative of your group to perform proper removal of PPEs
3. Submit in a Compact Disc or USB (as prescribed by the instructor)

0 = needs improvement: did not perform

1 = fair: performed below the standard
3 = very good: performed within the standard
5 = excellent: performed beyond the standard

GROUP PERFORMANCE 0 1 3 5
Procedure

1. Remove contaminated gloves properly and discard it

into the biohazard container.
2. Wash hands properly.
3. Remove the mask
4. Remove the laboratory gown. Slip the fingers of one
hand inside the cuff of the gown and pull the gown
over the hand.
5. Using the hand covered by the gown, pull the gown
down over the other hand.
6. Pull the gown off your arms. Hold the gown away from
you and roll it into a ball with the contaminated side on
the inside of the ball.
7. Dispose the gown into the biohazard container or its
appropriate receptacle.
8. Remove the cap.
 9. Remove the laboratory shoes
Mastery
a. The participant was able to show mastery of proper
removal of PPEs
Creativeness

a. The video portrays proper removal of PPEs in a

unique laboratory-setting
Punctuality

a. Video was submitted on time, as deadline prescribed

by the instructor.

COMMENTS:

_________________________________________________________________________
_________________________________________________________________________
_________________________________________________________________________
MEDICAL LABORATORY SCIENCE PROGRAM

PRINCIPLES OF
MEDICAL LABORATORY SCIENCE 2

Name__________________________________ Points Earned:_________________________

Section ______________ Group _____________ Date ________________________________

LEARNING ACTIVITY NO. 6C

PROPER REMOVAL OF PPEs

II. STUDENT LEARNING OUTCOME EVALUATION (GROUP WORK - Maximum 60

points)

1. Perform proper removal of PPEs

0 = needs improvement: did not perform

1 = fair: performed below the standard
3 = very good: performed within the standard
5 = excellent: performed beyond the standard

INDIVIDUAL PERFORMANCE 0 1 3 5
Preparation
a. The student prepared all the necessary materials
Procedure

1. Remove contaminated gloves properly and discard it

into the biohazard container.
2. Wash hands properly.
3. Remove the mask
4. Remove the laboratory gown. Slip the fingers of one
hand inside the cuff of the gown and pull the gown
over the hand.
5. Using the hand covered by the gown, pull the gown
down over the other hand.
6. Pull the gown off your arms. Hold the gown away from
you and roll it into a ball with the contaminated side on
the inside of the ball.
7. Dispose the gown into the biohazard container or its
 appropriate receptacle.
8. Remove the cap.
9. Remove the laboratory shoes
Mastery
a. The participant was able to show mastery and
spontaneity of proper removal of PPEs
Punctuality

a. The student performed the procedure within the

allotted time

COMMENTS:

_________________________________________________________________________
_________________________________________________________________________
_________________________________________________________________________

MEDICAL LABORATORY SCIENCE PROGRAM

PRINCIPLES OF
MEDICAL LABORATORY SCIENCE 2

Name__________________________________ Points Earned:_________________________

Section ______________ Group _____________ Date ________________________________

LEARNING ACTIVITY NO. 6C

PROPER REMOVAL OF PPEs

III. STUDENT LEARNING OUTCOME EVALUATION (GROUP WORK - Maximum 40
points)
1. Interview medical technologists who are working in the hospital or research about
the proper donning and removing of PPEs at the (1)laboratory, (2)bedside,
(3)Intensive Care Unit and (4)Operating Room.
2. Perform the different donning and removing of PPEs
3. Create a video presentation
4. Submit in a Compact Disc or USB (as prescribed by the instructor)

Rubrics
Score 5 3 1 0
Accuracy Excellent and Performed well Performed well Did not perform
(Proper donning accurate; but with a few but with a lot of
and removing of performed procedural flaws procedural flaws
PPEs at beyond standard
laboratory)
Accuracy Excellent and Performed well Performed well Did not perform
(Proper donning accurate; but with a few but with a lot of
and removing of performed procedural flaws procedural flaws
PPEs at beyond standard
bedside)
Accuracy Excellent and Performed well Performed well Did not perform
(Proper donning accurate; but with a few but with a lot of
and removing of performed procedural flaws procedural flaws
PPEs at ICU) beyond standard
Accuracy Excellent and Performed well Performed well Did not perform
(Proper donning accurate; but with a few but with a lot of
and removing of performed procedural flaws procedural flaws
PPEs at OR) beyond standard
Spontaneity Showed mastery Showed less Showed a little Did not perform
and spontaneity mastery and mastery and
in performing the spontaneity in spontaneity in
procedures performing the performing the
procedures procedures
Clarity of Speech is Speech is Speech is rarely Did not perform
speech always clear and usually clear and clear and easy to
easy to easy to understand
understand understand
Creativity Setting is Setting is less Setting is not Did not perform
appropriate and appropriate appropriate for
unique the procedure
Punctuality Submitted on or Submitted a day Submitted 2 Did not submit
before the after the days or more
deadline deadline after the
deadline

COMMENTS:
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