Enzymes catalyze reactions in metabolic pathways by bringing substrates close together and orienting them for reaction. Metabolic pathways consist of multiple enzyme-catalyzed steps that transform substrates into products. Cells balance catabolic pathways that break down molecules and anabolic pathways that build them up through regulation of enzyme activity and feedback inhibition to control metabolite levels and energy production.
Enzymes catalyze reactions in metabolic pathways by bringing substrates close together and orienting them for reaction. Metabolic pathways consist of multiple enzyme-catalyzed steps that transform substrates into products. Cells balance catabolic pathways that break down molecules and anabolic pathways that build them up through regulation of enzyme activity and feedback inhibition to control metabolite levels and energy production.
Enzymes catalyze reactions in metabolic pathways by bringing substrates close together and orienting them for reaction. Metabolic pathways consist of multiple enzyme-catalyzed steps that transform substrates into products. Cells balance catabolic pathways that break down molecules and anabolic pathways that build them up through regulation of enzyme activity and feedback inhibition to control metabolite levels and energy production.
2 | Metabolism and Enzymes are flexible proteins that change shape
when they bind with substrate molecules. In fact, Metabolic Pathways this binding and shape changing ability is how enzymes manage to increase reaction rates. In many cases, enzymes function by bringing two substrates into close proximity and orienting them for easier electron transfer. Shape or conformational changes can also act as an on/off switch. For example, when inhibitor molecules bind to a site on an enzyme distinct from the substrate site, they can make the enzyme assume an inactive conformation, thereby preventing it from catalyzing a reaction. Conversely, the binding of activator molecules can make an enzyme assume an active conformation, Cell Metabolism essentially turning it on. A cell's daily operations are accomplished through What Are Metabolic Pathways? the biochemical reactions that take place within the cell. Reactions are turned on and off or sped Many of the molecular transformations that occur up and slowed down according to the cell's within cells require multiple steps to accomplish. immediate needs and overall functions. At any Recall, for instance, that cells split one glucose given time, the numerous pathways involved in molecule into two pyruvate molecules by way of a building up and breaking down cellular ten-step process called glycolysis. This components must be monitored and balanced in coordinated series of chemical reactions is an a coordinated fashion. To achieve this goal, cells example of a metabolic in which the product of organize reactions into various enzyme-powered one reaction becomes the substrate for the next pathways. reaction. Consequently, the intermediate products of a metabolic pathway may be short-lived. What Do Enzymes Do? Sometimes, the enzymes involved in a particular Enzymes (Links to an external site.)are protein metabolic pathway are physically connected, catalysts that speed biochemical reactions by allowing the products of one reaction to be facilitating the molecular rearrangements that efficiently channeled to the next enzyme in the support cell function. pathway. For example, pyruvate dehydrogenase is Recall that chemical a complex of three different enzymes that reactions convert substrates into products, often catalyze the path from pyruvate (the end product by attaching chemical groups to or breaking off of glycolysis) to acetyl CoA (the first substrate in chemical groups from the substrates. For the citric acid cycle). Within this complex, example, in the final step of glycolysis, an enzyme intermediate products are passed directly from called pyruvate kinase transfers a phosphate one enzyme to the next. group from one substrate (phosphoenolpyruvate) to another substrate (ADP), thereby generating pyruvate and ATP as products. How Do Cells Keep Chemical Reactions in concentrations of key metabolites in the cell. For Balance? example, a cell may take stock of its levels of intermediate metabolites and tune the glycolytic Cells are expert recyclers. They disassemble large pathway and the synthesis of glucose accordingly. molecules into simpler building blocks and then In some instances, the products of a metabolic use those building blocks to create the new pathway actually serve as inhibitors of their own components they require. The breaking down of synthesis, in a process known as feedback complex organic molecules occurs via catabolic inhibition (Figure 5). For example, the first pathways and usually involves the release of intermediate in glycolysis, glucose-6-phosphate, energy. Through catabolic inhibits the very enzyme that produces it, pathways, polymers such as proteins, nucleic hexokinase. acids, and polysaccharides are reduced to their constituent parts: amino acids, nucleotides, and Conclusion sugars, respectively. In contrast, the synthesis of The management of biochemical reactions with new macromolecules occurs via anabolic enzymes is an important part of cellular pathways that require energy input . maintenance. Enzymatic activity allows a cell to Cells must balance their catabolic and anabolic respond to changing environmental demands and pathways in order to control their levels of regulate its metabolic pathways, both of which are critical metabolites — those molecules created by essential to cell survival. enzymatic activity — and ensure that sufficient DISCUSSION: METABOLIC PATHWAY energy is available. For example, if supplies of glucose start to wane, as might happen in the A metabolic pathway is a linked series of chemical case of starvation, cells will synthesize glucose reactions (Links to an external site.) occurring from other materials or start sending fatty acids within a cell (Links to an external site.). The into the citric acid cycle to generate ATP. reactants (Links to an external site.), products, and Conversely, in times of plenty, excess glucose is intermediates of an enzymatic reaction are known converted into storage forms, such as glycogen, as metabolites (Links to an external site.), which starches, and fats. are modified by a sequence of chemical reactions How Do Cells Manage All Their Chemical catalyzed (Links to an external site.) by enzymes (Links to an external site.). In most cases of a Reactions? metabolic pathway, the product (Links to an Not only do cells need to balance catabolic and external site.) of one enzyme acts as the substrate anabolic pathways, but they must also monitor (Links to an external site.) for the next. However, the needs and surpluses of all their different side products are considered waste and removed metabolic pathways . In order to bolster a from the cell. These enzymes often require dietary particular pathway, cells can increase the amount minerals, vitamins, and of the cofactors to of a necessary (rate-limiting) enzyme or use function. activators to convert that enzyme into an active conformation. Conversely, to slow down or halt a pathway, cells can decrease the amount of an There are two types of metabolic pathways that enzyme or use inhibitors to make the enzyme are characterized by their ability to either inactive. synthesize molecules with the utilization of energy Such up- and down-regulation of metabolic (anabolic pathway (Links to an external site.)) or pathways is often a response to changes in break down of complex molecules by releasing energy in the process (catabolic pathway (Links to an external site.)). The two pathways complement each other in that the energy released from one is used up by the other. The degradative process of a catabolic pathway provides the energy required to conduct a biosynthesis of an anabolic pathway.In addition to the two distinct metabolic pathways is the amphibolic pathway, which can be either catabolic or anabolic based on the need for or the availability of energy.
Pathways are required for the maintenance of
homeostasis (Links to an external site.) within an organism (Links to an external site.) and the flux (Links to an external site.) of metabolites through a pathway is regulated depending on the needs of the cell and the availability of the substrate. The end product of a pathway may be used immediately, initiate another metabolic pathway or be stored for later use. The metabolism (Links to an external site.) of a cell consists of an elaborate network of interconnected pathways that enable the synthesis and breakdown of molecules (anabolism and catabolism)..