Intructions:

1. Make a summary of key reactions for enterobacteriaceae.

The Enterobacteriaceae are aerobic and facultatively  Enterobacter,

anaerobic, non– spore-forming, nonmotile or peritrichously  Klebsiella,
flagellated, oxidase-negative, gram-negative bacilli that produce
 Citrobacter, and
acid fermentatively from glucose and reduce nitrates to nitrites.
Enterobacteriaceae are found on plants, in soil, in water, and in  Serratia.
the intestines of humans and animals. They have been
They act as opportunistic pathogens when they
associated with many clinical infections, including abscesses,
are introduced into body locations where they are not normally
pneumonia, meningitis, septicemia, and urinary tract infections
found, especially if the host is debilitated or immunosuppressed.
(McPherson & Pincus, 2017). They all cause the same types of opportunistic infections,
Several genera of Enterobacteriaceae are associated namely:
with gastroenteritis and food-borne disease. These include:
 Salmonella,  urinary tract infections,
 Shigella,  wound infections,
 certain strains of Escherichia coli, and  pneumonia, and
 certain species of Yersinia.  septicemia.

Many other genera of the family Enterobacteriaceae are normal

microbiota of the intestinal tract and are
considered opportunistic pathogens. The most common genera
of Enterobacteriaceae causing opportunistic infections in
humans are:
 Escherichia coli,
 Proteus,

1
2. Using the table provided below, list down the members of family enterobacteriaceae that exhibits the following reactions:

Lactose Negative H2S Positive VP Positive PAD Positive Urease Positive Non Motile @ 35℃

1. Pantoea spp. 1. Salmonella spp. 1. Klibsiella spp. 1. Morganella spp. 1. Klebsiella 1. Shigella spp.
pneumoniae
2. Proteus spp. 2. Citrobacter spp. 2. Serratia spp 2. Proteus spp. 2. Escherichia coli
2. Klebsiella oxytoca
3. Morganella spp. 3. Proteus spp. 3. Enterobacter spp. 3. Providencia spp. 3. Klebsiela spp.
3. Enterobacter
4. Providencia spp. 4. Morganella spp. 4. Pantoea spp. 4. Klebsiella spp. 4. Yersinia spp.
cloacae
5. Shigella spp. 5. Edwardsiella spp 5. Morganella spp.
4. Proteus spp.
6. Salmonella spp 6. Yersinia spp
5. Morganella spp.
7. Edwardsiella spp.
6. Providencia rettgeri
8. Yersinia spp
7. Yersinia
9. Serratia spp. enterocolitica

10. Escherichia coli

11. Klebsiella spp.
12. Enterobacter
spp.
13. Citrobacter spp.
Differentiation of Aerobic Gram-Negative Bacilli

Tes Escherichi Klebsiella Klebsiella Proteu Shigell Citrobacter Yersinia Enterobacter Serratia
t a coli pneumonia oxytoca s a spp. freudii enterocolitic cloacae marcescens
e vulgari a
s