DETERMINATION OF THE ANTIBACTERIAL PROPERTY…

Endorsement

This thesis entitled “Determination of the Antibacterial Property of Flavonoids Isolated from the
Leaf Extract of Momordica Cochinchinensis (Balbas-bakiro)” prepared by Marianne Judea J.
Mariano, Loriemie Ann G. Gatela, Karizza Jade R. Legaspi, Ahrizza P. Okuyama, Joshua D.
Turla and Wence Jerline F. Villoso, in partial fulfillment of the requirements for the degree
Bachelor of Science in Pharmacy has been examined and now recommendation for Oral
Examination.

This is to certify that Marianne Judea J. Mariano, Loriemie Ann G. Gatela, Karizza Jade R.
Legaspi, Ahrizza P. Okuyama, Joshua D. Turla and Wence Jerline F. Villoso are ready for the
Oral Examination

Dr. Angelita A. Rodriguez, Rph., MSPharm,.

Adviser

This is to certify that the thesis: “Determination of the Antibacterial Property of Flavonoids
Isolated from the Leaf Extract of Momordica Cochinchinensis (Balbas-bakiro)” prepared and
submitted by Marianne Judea J. Mariano, Loriemie Ann G. Gatela, Karizza Jade R. Legaspi,
Ahrizza P. Okuyama, Joshua D. Turla and Wence Jerline F. Villoso of Ph4Y1-3, is recommended
for Oral Presentation.

Dean Olive De Vera, RPh., MSPharm

Chair
______________________ ________________________
Panel Panel

Dr. Michael Joseph S. Dino, PhD., MAN, RN

Research Director
OLFU, Research Development and Innovation Center
Certificate of Originality

OUR LADY OF FATIMA UNIVERSITY COLLEGE OF PHARMACY

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Certificate of Originality

We hereby declare that this thesis is our own work and that, to the best of our knowledge and
belief, it contains no material previously published or written by another person nor material to
which to a substantial extent has been accepted for award of any other degree or diploma of a
university or other institute of higher learning, except where due to acknowledge is made in the
text.

We also declare that the intellectual content of this thesis is the product of our work, even though
we may have received assistance from others on style, presentation and language expression.

Marianne Judea J. Mariano

Principal Researcher

Loriemie Ann G. Gatela

Karizza Jade R. Legaspi

Ahrizza P. Okuyama

Joshua D. Turla

Wence Jerline F. Villoso

Members

Dr. Angelita A. Rodgiguez,

RPh, MSPharm Adviser

July14, 2018
Date Signed

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TABLE OF CONTENTS

PRELIMINARY PAGES PAGES

Title page
Endorsement i
Certificate of Originality ii
Table of contents iiii
List of Figures v
List of Tables vi
List of Appendices vi

ABSTRACT 1

1.0 INTRODUCTION 1
1.1 Statement of the Problem 2
1.2 Hypothesis 2
1.3 Significance of the Study 3
1.4 Scope and Limitation 4

2.0 REVIEW OF LITERATURE AND STUDIES

2.1 Theoretical Framework 4
2.2 Research Paradigm 5
2.3 Related Literature 5
2.3.1 Local Literature (See Appendix 1)
2.3.2 Foreign literature (See Appendix 1)
2.4 Related Studies
2.4.1 Local Studies (See Appendix 1)
2.4.2 Foreign Studies (See Appendix 1)

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3.0 METHODOLOGY
3.1 Research Design 6
3.2 Locale of Study 6
3.3 Research Ethics 6
3.4 Research Instruments 6
3.5 Data Collection
3.5.1 Collection and Preparation of Plant Extract 7
3.5.2 Phytochemical Screening 7
3.6 Fourier Transform Infrared Spectroscopy 9
3.7 Semi Purification of Flavonoids 9
3.8 Confirmatory test for flavonoids 9
3.9. Organoleptic Analysis 9
3.10 Solubility test 10
3.11 Microbiological test 10
3.11.1 Agar well diffusion 10
3.11.2 Disk Diffusion 10
3.11.3 Minimum Inhibitory Concentration 11
3.11.4 Inhibition zone 11

4.0 Results
4.1 Percentage Yield 12

4.2 Phytochemical Screening 13

4.3 Organoleptic Test 14
4.4 Solubility Test 14
4.5 Antibacterial Assay
4.5.1 Disk Diffusion 15
4.5.2 Minimum Inhibitory Concentration 17
4.6 Statistical Treatment 18
5.0 Summary 20
6.0 Conclusion 22

7.0 Recommendation 23

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8.0 References 24

9.0 Glossary of Terms 34

LIST OF FIGURES

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Figures Page

1 Research paradigm 6

2 Balbas bakiro (Momordica cochinchinensis) 7

3 Balbas bakiro leaves 41

4 Drying of Balbas bakiro leaves 41

5 Maceration of the Balbas bakiro leaf 42

6 Filtration of the Balbas bakiro leaf extract 43

7 Phytochemical screening of alkaloids 43

8 Phytochemical screening of flavonoids 44

9 Phytochemical screening of saponins 44

10 Phytochemical screening of tannins 45

LIST OF TABLES

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Tables Pages
1 Standard zones of inhibition and corresponding interferences
3 Result for percentage yield of Balbas-bakiro leaf 29
4 Phytochemical screenings result 29

LIST OF APPENDICES
Appendix 1 Related literature 38
Related Studies 43
Appendix 2 Documentation 47

OUR LADY OF FATIMA UNIVERSITY COLLEGE OF PHARMACY