1/12/2020 BAM Media M19: Bismuth Sulfite Agar (Wilson and Blair) | FDA
BAM Media M19: Bismuth Sulfite Agar (Wilson and Blair)
January 2001
Bacteriological Analytical Manual
M19
Bismuth Sulfite Agar (Wilson and Blair)
Polypeptone (or peptone) | 109
Beef extract 59
Dextrose 59
NagHPO« (anhydrous) 49
FeSO, (anhydrous) 039
Bismuth sulfite (indicator) | 8g
Brilliant green 0.0259
Agar 209
Distilled water ‘iter
Mix thoroughly and heat with agitation. Boil about 1 min to obtain uniform suspension.
(Precipitate will not dissolve.) Cool to 45-50°C. Suspend precipitate by gentle agitation, and
pour 20 ml portions into sterile 15 x 100 mm petri dishes. Let plates dry about 2 h with lids
partially removed; then close plates. Final pH, 7.7 + 0.2
DO NOT AUTOCLAVE. Prepare plates on day before streaking and store in dark.
Selectivity decreases in 48h.
CAUTION: DO NOT AUTOCLAVE
PREPARATION Day before use.
STORAGE In Dark
Loss of Selectivity after 48 h
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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