Scribd Logo
Upload

Welcome to Scribd!

  • 10 views

    Experiment 2 Pharmacognosy

    Uploaded by

    Abdullah Yousafzai
    Paper chromatography is a technique used to separate mixtures of chemical substances. It works by partitioning the substances between a stationary phase (filter paper) and a mobile phase (solvent). There are two types of paper chromatography - ascending, where the solvent rises up the paper via capillary action, and descending, where gravity and capillary action cause the solvent to flow down the paper. To perform paper chromatography, samples are spotted onto filter paper and developed with a suitable solvent. The distance traveled by each substance is used to calculate its retention factor (Rf value) for identification.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content

    You might also like

    Experiment 2 Pharmacognosy

    Uploaded by

    Abdullah Yousafzai
    10 views2 pages
    Paper chromatography is a technique used to separate mixtures of chemical substances. It works by partitioning the substances between a stationary phase (filter paper) and a mobile phase (solvent). There are two types of paper chromatography - ascending, where the solvent rises up the paper via capillary action, and descending, where gravity and capillary action cause the solvent to flow down the paper. To perform paper chromatography, samples are spotted onto filter paper and developed with a suitable solvent. The distance traveled by each substance is used to calculate its retention factor (Rf value) for identification.

    Original Description:

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    Paper chromatography is a technique used to separate mixtures of chemical substances. It works by partitioning the substances between a stationary phase (filter paper) and a mobile phase (solvent). There are two types of paper chromatography - ascending, where the solvent rises up the paper via capillary action, and descending, where gravity and capillary action cause the solvent to flow down the paper. To perform paper chromatography, samples are spotted onto filter paper and developed with a suitable solvent. The distance traveled by each substance is used to calculate its retention factor (Rf value) for identification.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as docx, pdf, or txt
    10 views2 pages

    Experiment 2 Pharmacognosy

    Uploaded by

    Abdullah Yousafzai
    Paper chromatography is a technique used to separate mixtures of chemical substances. It works by partitioning the substances between a stationary phase (filter paper) and a mobile phase (solvent). There are two types of paper chromatography - ascending, where the solvent rises up the paper via capillary action, and descending, where gravity and capillary action cause the solvent to flow down the paper. To perform paper chromatography, samples are spotted onto filter paper and developed with a suitable solvent. The distance traveled by each substance is used to calculate its retention factor (Rf value) for identification.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as docx, pdf, or txt
    of 2

    Paper chromatography for the separation of components

    Chromatography:

    It is a technique for examination and separation of mixtures of chemical substances. It


    facilitates purification, isolation, and comparison of various components. It can be
    undertaken for all kind of volatile and soluble substances, organic and inorganic, polar
    and nonpolar, in different quantities under various conditions. Different substances are
    partitioned differently between two phases (one is stationary phase and the other is
    mobile phase)

    Paper chromatography:

    A drop or spot of solution containing the mixture of substances to be separated and


    applied near one end of filter paper piece. The paper is carried out into two ways

    1. Ascending paper chromatography


    2. Descending paper chromatography

    Ascending paper chromatography:

    In ascending paper chromatography the solvent is given in a pool at the bottom of the
    vessel in which the paper is supported. The solvent rises up by the capillary action

    Descending paper chromatography:

    The solvent is in trough form in which the paper is placed. The solvent flows down the
    paper by combination of gravity action and capillary action.

    Requirements

    Beaker, filter paper, capillary tube, inks

    Procedure

    Select the suitable size of filter paper and pencil.

    Draw the parallel line to one end of paper.

    A number of small spots are marked at suitable distance on this line at equal distance.
    The sample no. or nature of sample to be applied is written against each cross with
    pencil. A drop of each sample is spotted at appropriate portion with capillary spots are
    allowed to dry until the solvent is completely evaporated. The end of paper near the
    spot is immersed in a suitable solvent for certain period of time

    The solvent is displaced from origin through capillary action.

    Calculate the Rf value

    Precaution:

    Large spot should be avoided as they may lead to poorer separation.

    The filter paper should be haug properly so that the separation is appropriate.

    Do not immersed the spot in the solvent.

    You might also like