NAME: MARWIN R.

DELA CRUZ RATING: _____________

YEAR & SECTION: 2B CRIMINOLOGY DATE: SEPTEMBER 2, 2020

EXPERIMENT NO. 1
THE COMPOUND MICROSCOPE

I. OBJECTIVE: TO GIVE THE PARTS AND FUNCTIONS OF MICROSCOPE

TO KNOW THE TECHNIQUE OF USING THE MICROSCOPE

II. MATERIALS: Microscope, glass slide and cover slip

III. OBSERVATION:
Draw and label the parts of microscope.
IV. CONCLUSION:
1. Give the function of the following:
a. Revolving nose piece- A microscope is an important part of a standard optical
microscope. Used in classrooms and labs, the optical microscope is the most
popular form of microscope due to its low cost relative to other types of
microscope as well as its simplicity. A user of an optical microscope should learn
about the revolving nose piece in order to properly use the microscope.
b. Course adjustment-

c. Condenser

d. Stage

e. Iris diagram

f. Mirror

g. Ocular or eyepiece

h. High power objective

2. Describe briefly and in systematic order the manner or method of properly usage
of microscope.

3. Give some of the precautionary measures in the use of the microscope.

4. Name the three general parts of microscope and give example.

IV. Conclusion

1. Give the function of the following:

A. Revolving nose piece-It is responsible for holding the objective lenses. They can be used in
order to change the magnification of the microscope well.
B. Course adjustment-The coarse adjustment is used to first bring the object in to approximate
focus starting first with the stage as close to the objective lens without touching.
C. Condenser-The function of the condenser in are frigeration system is to transfer heat from
the refrigerant to another medium, such as air and/or water. By rejecting heat, the gaseous
refrigerant condenses to liquid inside the condenser. The major type soft condensers use dare
(1) water-cooled,(2)air-cooled, and(3)evaporative.
D. Stage-The flat platform that supports the slides. Stage clips hold the slides in place. If your
microscope has a mechanical stage, the slide is controlled by turning two knobs instead of
having to move it manually. One knob moves the slide left and right, the other move sit forward
and backward.
E Iris diaphragm- it is used to control the amount of light reaching the specimen. It is located
above the
Condenser and below the stage. Most high quality microscopes include an Abbe condenser
with an iris diaphragm.
Combined, they control both the focus and quantity of light applied to the specimen.
F. Mirror-It is used to reflect light from an external light source up through the bottom of the
stage. Nosepiece: This circular structure is where the different objective lenses are screwed in.
to change the magnification power,
Simply rotate the turret.
G. Ocular or eyepiece-The eye pieces are the lenses at the top that the viewer looks through;
they are usually 10 X or 15X. Use to get the total magnification level, multiply the magnification
of the objective used (ex: 10X eyepiece *40X objective=400X total magnification).
H. High power objective- It is ideal for observing the small details within a specimen sample.
The total magnification for this lens is equal to 400x magnification (10x eyepiece lens x the 40x
objective equals 400).

2. Describe briefly and in systematic order the manner or method of properly usage of
microscope.

HOW TO USE A PROPERLY USE A MICROSCOPE

Basics
1. When transporting the microscope, carry it close to your body with one hand on the arm and
the of her on the base. If you are unsure what part of the microscope is which, Ire commend
starting a tour Microscope Parts & Functions section, found here.
2. Once you have removed the dust cover from the microscope if using one, and have plugged
the microscope in, arrange the excess cord in a manner that will prevent you from tripping on it
and knocking the microscope over. A microscope is a precision instrument, so any sharp
movement so impact can set the fine elements of the unit off.
3. As a general rule, always start and end with the lowest power objective on the microscope
(usually4X), as it is easiest to focus and center the sample in the image on a lower power. Also,
since it is the shortest objective lens, there is the least chance of scratching the lens when
situating or removing the slide from the microscope. The below images how show to properly
mount the sample on to your slide.
4. Turn on the microscope and place the slide on the microscope stage with the specimen
directly over the circle of light. Doing this will give you a 90% chance of finding the specimen as
soon as you look through the eyepiece. If your microscope is monocular (it has only one
eyepiece), close or cover your o there ye for accuracy and comfort when viewing. If your
microscope is binocular, adjust the inter pupillary distance (the distance between the
Eyepieces) by either sliding or rotating the eyepieces (called adjusting the diopter if present on
the microscope) appropriately until you can see only one circle of light with both eyes open.
NOTE: If you wear glasses, take them off; if you see only your eyelashes, move closer. If your
microscope comes with high-eye point eyepieces (like those of our SM and ZM stereo zoom
microscopes),you do not need to take off your glasses.
5. If you are on the lowest magnification level, focus the image by first turning the coarse focus
knob. If you can’t get it at all in to focus using the coarse knob, then switch olefin focus knob.
6. Adjust the diaphragm as you look through the eyepiece. You will begin to notice that more
detail is visible when you allow in less light. Too much light tends to give the specimen a
washed-out appearance.
7. Once you have focused the specimen on low power (usuallya4Xobjective), center the
specimen in your field of view, then, without changing the focus knobs, switch it to a higher
magnification objective (10Xobjective, then 40Xobjective). If you don’t center the specimen you
will lose it when you switch to higher powers.
8. Once you have it on a higher power magnification, remember to only use the fine focus knob
to bring the image in to focus. This, if present, is typically the smaller knob on the microscope,
and will move the stage a much shorter distance than the coarse focusing knob. Since the
higher power objectives are very closet the slide, using the coarse focus knob can scratch the
lens and crack the slide.
9. Use the 100X objective with extreme care. As it is an oil immersion lens, using it without the
oil to lubricate the Lens can destroy the lens and provide an unclear image of your sample.
Place one drop of oil between the cover slip of the slide and the objective lens. You may slowly
add more until the oil fills the air gap between the objective lens and the slide. The oil will also
help gather enough light to actually see through the lens and to prevent distortion of the image.
For more detailed information on how to use your oil immersion lens, feel free to email us here
requesting a manual for the technique. You can also find some instructions in video form on
You Tube by searching for "Oil immersion Microscopy "or something similar.

1. Gather at hinslice/piece of your specimen. If your specimen is too thick, the cover slip will not
be level and will wobble on top of the sample. The below image is an example of an incorrect
mounting of the cover slip that will
Make focusing on the sample difficult.
2. Place ONE drop of water directly over the specimen. Too much water over the specimen will
cause the cover slip will float on top of the water, causing the specimen to float in and out of
your field of view.
3. Place the cover slip at approximately a 45 degree angle to the slide with one edge touching
the water drop. Let go and allow the cover slip to fall on to the specimen.

1. Place one drop of Methylene Blue (or other stain, sold separately from another vendor) on
one edge of the cover slip, and place the flat edge of a piece of paper towel on the other edge.
2. As the paper towel draws the water out from under the cover slip on one side, the flow of
liquid will draw the stain in under the cover slip from the other side.
3. As soon as the stain has covered the area containing the specimen, you are done. The stain
does not need to be under the entire coverslip. If the stain does not cover the desired area,
repeat the process with a new piece of paper towel and more stain.
4. Be sure to wipe of the excess stain with a paper towel, so you don’t end up permanently
staining the objective lenses.
5. Now you are ready to place the slide on the microscope stage. Be sure to follow all previous
instructions on how to property operate a microscope.
6. Be sure to wash and dry both the slide and the cover slip after use.
If you still have more questions after reviewing this page, please call us at(888)950-2888 and
someone neat our Company an assist you, or send us an email here.

Basic (B100, M500 Style) Compound Microscope: Use the tool below, which comes with most
microscopes, to adjust the focusing tension according to the picture to the right.
Stereo Zoom (SM &ZM) Microscopes: On most SM and ZM microscopes, the tension system is
located on the focusing rack rather than the head. The focusing rack's dual knobs need to be
rotated in opposite directions to increase or decrease the tension in the system.
Advanced (600&800 Series) Compound Microscopes: There are two different styles on our
advanced models. For the 680&800 series models, Use the wrench below to loose nor tighten
the focusing tension. For the other compound microscope models (490&up), there is a ring on
the inner section of the right hand focusing knob. This ring can be turned by hand to increase or
decrease the tension. There is a small screw on the side of the eye tube. Use a tiny screw
driver (typically 1mm precision screw driver size) to remove the screw so that you can take the
eyepiece out and insert a new one. If you need to turn the microscope upside down for any
reason, make sure to tighten this screw, or remove the eyepiece first to prevent accidental
damage.
Put the color filter in the swing-out ring holder if present on the microscope. Typically this style
is found on models with an Abbe condenser and adjustable iris. If the swing holder is facing the
body of the microscope, simply rotate the entire condenser assembly until it swings to the front.
Note: Microscopes with a disc style iris condenser typically do not have a filter holder. In this
case, the filter may simply be placed and centered on the base illuminating lens or cover of the
microscope.
600or680SeriesMicroscopeHeadInstallation
Use the he wrench to loose nor tighten the locking screw.

Then eye-guard is foldable. This allows you to block ambient light from hitting your eyepieces
when using a stereo microscope, preventing any double images or other viewing issues.
https://www.amscope.com/how-to-use-microscope
3. Give some of the precautionary measures in the use of the microscope.
I. Clean the microscope after each use.
a. Clean smudged lenses with lens paper. Avoid applying pressure with a cloth as the lenses
are very fragile.
b. Wipe the stage (the platform that holds the slides) down thoroughly and disinfect the
eyepiece with an alcohol-based wipe.
II. Handle glass slides carefully. If a slide breaks, ensure that the contents are properly
disposed and report the incident in People Soft if an injury occurs.
III. Turn off the light source when the microscope is not in use. This will improve l amp longevity
and save energy.
IV. Be aware if your microscope has a mercury lamp. A broken mercury lamp may release toxic
mercury vapors. You can dispose of any unwanted mercury-containing devices by submitting a
pick-up request to chemwaste@emory.edu
V. When carrying the microscope, always use two hands with one hand supporting the base
and the other hand holding the arm.
VI. Properly store the microscope by lowering the nosepiece, turning off the light source, and
placing the objective lenses on the lowest setting. Cover with a dust jacket.
VII. Ensure your microscope is scheduled for preventative maintenance and keep the area
around the microscope clean. For more information, consult the manufacturer’s manual for your
equipment
4. Name the three general parts of microscope and give example. The three basic, structural
component sofa compound microscope are the head, base and arm.
1. Head/Body houses the optical parts in the upper part of the microscope.
2. Base of the microscope supports the microscope and houses the illuminator.
3. Arm connects to the base and supports the microscope head.