BE132P Instrumentation in Biological Engineering 1

Name: CUEVAS, Bernadette V. Date:

Student Number: 2015102318 Program/Year: BE/4

EXERCISE 3: MICROSCOPY TECHNIQUES

Objective: Familiarization of the parts and functions of a Phase Contrast Microscope and a Fluorescence
Microscope.

1. Type of Microscope: PHASE CONTRAST MICROSCOPE

Identify the parts of the given microscope and briefly describe its function/s.
Label
Part Description/s
#
This is where viewers look through to observe the
1 Eyepiece
specimen.
Segregates diffracted lights that was collected by
2 Phase Plate
the objective
3 Phase Contrast Objective Lens Collects light from the light source
4 Stage Where specimens are clipped
Where partially coherent light produced by the
5 Condenser Annulus
tungsten-lamp are focused
BE132P Instrumentation in Biological Engineering 1

Utilizes wide range magnifications, as well as

6 Condenser accessories for varying contrast-enhancement
techniques
7 Tungsten Lamp Light source of the microscope

2. Type of Microscope: Fluorescence Microscope

Identify the parts of the given microscope and briefly describe its function/s.
Label
Part Description/s
#
Used as camera sensor that captures photons
1 Peltier-Cooled CCD Camera
reflected off of an object and converts to image
2 Eyepiece Specimens are viewed through this
With the addition of the half-reflecting surface,
3 Vertical (Episcopic) Illuminator Vertical Illuminator provides true on-axis
illumination
4 Breathshield (UV Shield) Protects the operator from the harmful short-
wavelength of UV radiation when the shutter is
BE132P Instrumentation in Biological Engineering 1

open
Gathers fluorescence emitted that is produced by
5 Objective
illuminating specimen
6 Stage Where specimens are placed for observation
Accommodates between four and six fluorescence
7 Condenser Turret
cubes
Spreads light and creates sufficient light field for
8 Field Lens
establishing Kohler illumination
9 Base Serves as stand of the microscope
Separates excited illumination from fluorescence
10 Transmitted Light Filters
emission
11 Field and Aperture Diaphragms Dictates illumination intensity and field size
Most common light source for fluorescence
12 Mercury (HBO) Lamps
microscope
13 Microscope Frame Protects the microscope circuit board
14 Lamp Intensity Control Controls light intensity from the light source
15 Tungsten Halogen Lamphouse Serves as light source