MLS 112 - Hema 1

DR. CARLOS S.
LANTING COLLEGE
Medical Laboratory Science Department
16 Tandang Sora Ave., Sangandaan, Novaliches, Quezon City
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
Email: info@lantingcollege.edu.ph Website: www.lantingcollege.edu.ph
CMO no. 13 series of 2017
COURSE CODE COURSE TITLE
MLS 112 Hematology 1 (Clinical Hematology)
Department: Medical Technology / MLS Revision Status:

Prepared by: Verified by:
DR. GABRIEL J. CRUZ PROF. JOHN DALE B. DURANO
Reviewed by: Approved by:
PROF. MAYLAINE M. DE LEON DR. CARLOS S. DE LEON
DCLC VISION 2025 DCLC aims to be one of the country’s leading colleges in providing quality education
DCLC MISSION To produce competent graduates who can contribute to nation building.
Service for the welfare and empowerment of others;
Excellence, comparable to highest standards, while having strong moral principles;
CORE VALUES Resilience in all challenges;
Virtue as a guiding principle, admired by others as God loving individuals;
Integrity, honesty and strong belief on one’s
Commitment in responding to the community’s needs; and
Empathy in valuing social fairness.
INSTITUTIONAL Graduates of Dr. Carlos S. Lanting College are globally comparative, competitive, and
OUTCOMES competent in their field of profession, with high level of humanistic values, critical thinking,
conceptual and technical skills in accordance with the standards of academic program and
industry requirements relevant to nation building.
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DR. CARLOS S. LANTING COLLEGE
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
PROGRAM The graduates have the ability to:

OUTCOMES a. Demonstrate technical competence in performance of clinical laboratory test in aid
of diagnosis, treatment, and management of diseases vis-à-vis biosafety and waste
management;
b. Demonstrate analytical and critical thinking skills in the workplace;
c. Engage in the collection, analysis, and projection of health information for
improving the health care management system;
d. Demonstrate inter-personal skills, leadership qualities, and ethical practice of the
profession;
e. Apply research skills in relevant areas of Medical Technology/Medical Laboratory
Science practice;
f. Participate in community-oriented activities;
g. Engage in life-long learning activities; and
h. Demonstrate effective teaching and communication skills.
COURSE This course deals with the study of blood as a tissue and the pathophysiology of the cellular
DESCRIPTION elements of the blood. Emphasis is on laboratory diagnostic procedures.
CREDIT UNIT/S 5 units (Lecture: 3, Laboratory: 2)
CREDIT HOUR/S Lecture: 3 hours (54 hours per sem.)
Laboratory: 6 hours ( 108 hours per sem. )
PREREQUISITE Biochemistry, Human Anatomy and Physiology
COURSE CILO 1 Explain the facts and principles of hematological determinations.
INTENDED CILO 2 Differentiate microscopically the normal and abnormal blood cells.
LEARNING CILO 3 Correlate hematological tests to pathologic conditions.
OUTCOMES CILO 4 Perform hematological tests with precision, accuracy and reliability.
CILO 5 Assume responsibility in handling blood specimens, including examination and
interpretation of test results.
CILO 6 Manifest the following values and skills: independence, integrity, honesty, critical
thinking, empathy and value for life.
COURSE This course will be delivered entirely online through the course management system
STRUCTURE CANVAS and support apps like EDMODO and ZIP Grade. You will use your student account
to login to the course from the CANVAS. EDMODO AND ZIP GRADE Login Page. If you
have not activated your student account, please visit the Manage Your Account on our MLS
FB page to do so.
In CANVAS, you will access online lessons, course materials, and resources. At designated
times throughout the semester, we will participate in a blend of self-paced and group-paced
activities using and/or alternative Internet-based technologies. Activities will
consist of chat, blogs, discussion forums, email, journaling, blogging, wikis, and web posting.
COURSE POLICIES Netiquette is a set of rules for behaving properly online. Your instructor and fellow students
wish to foster a safe online learning environment. All opinions and experiences, no matter
how different or controversial they may be perceived, must be respected in the tolerant spirit
of academic discourse. You are encouraged to comment, question, or critique an idea but
you are not to attack an individual. Working as a community of learners, we can build a polite
and respectful course community.
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Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
COURSE As a student in this course you are expected to maintain high degrees of professionalism,
INTEGRITY commitment to active learning and participation in this class and also integrity in your
behavior in and out of the on line classroom.
COURSE All assignments for this course will be submitted electronically through CANVAS unless
ASSIGNMENTS otherwise instructed. Assignments must be submitted by the given deadline or special
permission must be requested from instructor before the due date. Extensions will not be
given beyond the next assignment except under extreme circumstances.
LECTURE
OCTOBER 19,2020 – NOVEMBER 13,2020
TIME FRAME COURSE OUTLINE
PRELIM
Day 1 Orientation
A. Introduction to the course contents
B. Course Requirements and Activities
C. Grading system and Policies on Absences, tardiness and examinations
Days 2-3 I. Introduction
1.1 Definition of terms
1.2 Characteristics of blood
1.3 Functions of blood
1.4 Composition
II. Basic hematological methods and examination

2.1 Collection, handling, and preservation of blood for hematologic study.
2.2 Evacuated tubes: Anticoagulants used in blood preservation
Days 4 -5 III. Hematopoiesis in general:
a. Erythropoiesis
b. Granulopoiesis
c. Lymphopoiesis
d. Monopoiesis
e. Megakaryopoiesis
IV. Hemoglobin
a. synthesis of heme and globin
b. types
c. routine determination and electrophoresis
V. Hematocrit
a. proper procedures
b. sources of error in the determination
VI. Erythrocyte Sedimentation Rate

c. clinical use
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VII. Osmotic Fragility Index

c. clinical use
Days 5 – 6 IX. Reticulocyte
a. Corrected reticulocyte count
b. Reticulocyte production index
X. Red Blood Cell Count

a. Materials and reagents used
b. Reference values
XI. Red cell metabolic pathways
XII. Red blood cell anomalies (including inclusion bodies, poikilocytes, and anisocytes)
XIII. Red Cell Distribution Width (RDW)
XIV. Red Blood Cell Indices (Mean Corpuscular Volume, Mean Corpuscular Hemoglobin, Mean
Corpuscular Hemoglobin Concentration)
a. computation
b. clinical use
Day 7 XV. Intravascular Hemolysis
a. Causes
b. Laboratory results observed
XVI. Extravascular Hemolysis

a. Causes
b. Laboratory results observed
XVII. Anemia
a. Mechanisms
b. Signs and symptoms
c. Laboratory results observed
Day 8 FIRST PERIOD EXAMINATION (PRELIM)
SECOND PERIOD NOVEMBER 16,2020 – DECEMBER 12,2020
Days 1- 5 XXI. White blood cell types and normal morphology
a. Neutrophils
b. Eosinophils
c. Basophils
d. Lymphocytes
e. Monocytes
XXII. White Blood Cell Count
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a. Materials and reagents used

b. Reference values
XXIII. Differential count

a. Materials
b. Reference ranges
c. Relative count versus absolute count
XXIV. White blood cell anomalies

a. Basket cells
b. Atypical lymphocyes
c. Hypersegmented neutrophils
d. Lupus erythematosus (LE) cell
e. Hairy cells
f. Pelger-Huet anomaly
g. Dohle bodies
h. Alder-Reilly anomaly
i. Toxic granulation
j. Czediak-Higashi Syndrome
k. May-Hegglin anomaly
l. Gaucher cell
m. Foam cell
n. Flame cell
o. Auer rods
p. OTHERS
Days 6 – 7 XXV. Leukemia
a. General overview
b. Leukemia vs. Leukemoid Reaction
c. French-American-British (FAB) Classification
d. World Health Organization (WHO) Classification
e. Cytochemistry
XXVI. Lymphomas
a. General overview
b. Hodgkin’s vs. Non-Hodgkin’s
Day 8 SECOND PERIOD EXAMINATION (MIDTERM)
LABORATORY
NOVEMBER 16,2020 – DECEMBER 12,2020
TIME FRAME COURSE OUTLINE
Day 1 – 2 Orientation and SOP’s
1. Collection of Blood Specimens
a. Venipuncture (with discussion on the new “Order of Draw” and the blood additives)
b. Skin puncture (for infants and adults)
c .Patient preparation for hematologic tests
Day 3 – 4 2. Hemoglobin determination
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Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
2. Hematocrit determination
3. ESR
4. OFT
Days 5 – 6 1. Hemocytometer
2. Blood Dilution
3. Red Blood Cell count
4. Reticulocyte count
Day 7 5. Erythrocyte Indices
Day 8 FIRST PERIOD EXAMINATION (PRELIM)
SECOND PERIOD NOVEMBER 16,2020 – DECEMBER 12,2020
Days 1 – 3 1. Blood Smear Preparation
2. Differential count
3. Identification of Blood Cells seen in the peripheral smear.
Days 4 – 7 1.Special Hematology Test
Introduction
a. L.E slide preparation
b. Bone marrow smear study
c. Alkali Denaturation Test
d. Test for Paroxysmal Nocturnal Hemoglobinuria
e. Test for Paroxysmal Cold Hemoglobinuria
Day 8 SECOND PERIOD EXAMINATION (MIDTERM)
COURSE Attendance – 80% of the required class hours
REQUIREMENTS Class participation – discussions, examination etc.
APPROACH/ Lecture / discussion
METHODOLOGY Visual aids
Oral recitation
EVALUATION Recitation
Research work / Seat work
Examination
GRADING SYSTEM LECTURE 60 % LABORATORY 40 %
Recitation 10 % Attendance 10 %
Short Quiz 10 % Short Quiz 10 %
Long Quiz 20 % Class Standing – Experiment -10 %
Class Standing – Reporting 10 % Class Standing – Laboratory Manual -10 %
Class Standing – Research / Project -10 % Practical Exam 30 %
Major Examination- 40 % Theoretical Exam 30 %
Total = 100 % Total = 100 %
Note: Final Grade = First Period + Second Period / 2

REFERENCE 1. Anderson S. and K. Poulsen. Anderson’s Atlas of Hematology. USA: Lippincott Williams
and Wilkins, 2003.
2. Brown, Barbara. Hematology: Principles and Procedures 6th ed. Philadelphia: Lea and
Febiger, 1993.
3. Carr, Jaqueline and Bernadette Rodak. Clinical Hematology Atlas 3rd ed. Philadelphia:
W.B. Saunders, 2008.
Page 6 of 8
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
4. Garza, Diana and Kathleen Becan-McBride. Phlebotomy Handbook: Blood Collection

Essentials 7th ed. USA: Prentice Hall, 2004.
5. Greer J., J. Foerster and J. Lukens. Wintrobe’s Clinical Hematology 11th ed. USA:
Lippincott Williams and Wilkins, 2003.
6. Harmening D. Clinical Hematology and Fundamentals of Hemostasis. 5th ed. USA: F.A.
Davis Co., 2008.
7. Hoeltke, Lynn. Phlebotomy: Principles and Procedures. USA: Delmar Learning, 2006.
8. Hoffbrand V., P. Moss and J. Pettit. Essential Haematology 5th ed. USA: Wiley-Blackwell,
2006.
9. Hoffman R. et.al. Hematology: Basic Principles and Practice 4th ed. USA: Churchill
Livingstone, 2004.
10. McClatchey, Kenneth. Clinical Laboratory Medicine 2nd ed. USA: Lippincott Williams
and Wilkins, 2002.
11. Mckenzie, Shirley, Clinical Laboratory Hematology. USA: Prentice Hall, 2006.
12. Mcpherson, Richard A. and Matthew R. Pincus. Henry’s Clinical Diagnosis and
Management by Laboratory Methods 21st ed. Philadelphia: Elsevier Inc., 2007.
13. Reddi, V., M. Marques and G. Fristma. Quick Guide to Hematology Testing. USA: AACC
Press, 2007.
14. Rodak B., G. Fritsma and K. Doiq. Hematology: Clinical Principles and Applications 3rd
ed. Philadelphia: W.B. Saunders, 2007.
15. Schmaier A. and L. Petruzelli. Hematology for the Medical Student. USA: Lippincott
Williams and Wilkins, 2003.
16. Scott M., A. Gronowski and C. Eby. Tietz’s Applied Laboratory Medicine 2nd ed. USA:
Wiley-Liss, 2007.
17. Steininger, Cheryl et al. Clinical Hematology: Principles, Procedures and Correlations.
Philadelphia: J.B. Lippincot, 1992.
18. Theml, H. Color Atlas of Hematology: Practical, Microscopic and Clinical Diagnosis 2nd
ed. USA: Thieme, 2004.
19. Turgeon, Mary Louise. Clinical Hematology: Theory and Procedures 4th ed. USA:
Lippincott Williams and Wilkins, 2004.
Electronic References:
1. http://wps.prenhall.com/chet_mckenzie_clinicallab.
2. http://www.bloodline.net/
3. http://www.emedicine.com/med/topic132.htm
4. http://www.hematologyatlas.com/
5. http://www.wadsworth.org/chemheme/heme/cytoheme/cyto35mm.htm
CLASS ROOM CLASS BEHAVIOR that disrupts the learning process will not be tolerated. This includes:
POLICY talking to others during lecture, demonstrations, quizzes or exams. Sleeping or laying the
head down during class, using cell phones for any reason, noises from electronic devices,
wearing headphones, swearing and rude or disrespectful behaviour. Cell phone usage of
any kind will not be allowed in class. Please turn off or put on silent mode upon entering the
class. Cell phones shall not be used for computations. Courtesy, kindness and respect are
expected at all times from students and faculty.
Page 7 of 8
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
ACADEMIC HONESTY: Cheating of any kind is a serious breach of personal morality and
an offense against fellow students, instructors/professors, program, and school. Any student
found to be academically dishonest will receive a Failing Grade and dismissed from the
program and school. Please refer to Student Handbook.
* The course schedule is subject to change with prior notice from the instructor.
* The major examinations schedule is subject to change with prior notice from the instructor.
Page 8 of 8

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DR. CARLOS S.

CMO no. 13 series of 2017

COURSE CODE COURSE TITLE

MLS 112 Hematology 1 (Clinical Hematology)

Department: Medical Technology / MLS Revision Status:

DR. GABRIEL J. CRUZ PROF. JOHN DALE B. DURANO

Reviewed by: Approved by:

PROF. MAYLAINE M. DE LEON DR. CARLOS S. DE LEON

PROGRAM The graduates have the ability to:

II. Basic hematological methods and examination

VI. Erythrocyte Sedimentation Rate

VII. Osmotic Fragility Index

X. Red Blood Cell Count

XI. Red cell metabolic pathways

XIII. Red Cell Distribution Width (RDW)

XVI. Extravascular Hemolysis

XXII. White Blood Cell Count

a. Materials and reagents used

XXIII. Differential count

XXIV. White blood cell anomalies

Note: Final Grade = First Period + Second Period / 2

4. Garza, Diana and Kathleen Becan-McBride. Phlebotomy Handbook: Blood Collection

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