TG–DSC analysis

Thermo gravimetric differential scanning colorimeter analysis was carried out using TG–
DSC (SDT Q600 model, TA Instruments, USA). About 5 mg of the sample was taken in an
alumina cup holder and heated up to 1000 ◦C at the rate of 10 ◦C/min.
Particle size measurement

Particle sizing experiments were carried out by means of laser diffractometry, using Zeta
sizer nano-series (Malvern). Measure- ments were taken in the range between 0.1 and
10,000 nm.
Antimicrobial activity

Anti-microbial activity of the AgNPs was screened against Staphylococcus aureus and
Escherichia coli by Baur et al.’s method. Briefly, Overnight cultures of the bacterial strains
were prepared in Luria broth. 150 µL (100 µg/ml) of the synthesized AgNPs were filled in to
the well and kept for incubation overnight at 37 ◦C.
 350.0 360 380 400 420 440 460 480.0
nm
Wavelength (nm)
Fig. 2. UV–vis spectra of synthesized AgNPs.

Table 1
Phytochemical screening of plant extract and synthesized nanoparticles.

S. no. Chemical constituents Screening

Plant extract AgNps

1 Alkaloids + –
2 Tannins + +
3 Carbohydrates + +
4 Ascorbic acid + +
5 Proteins + +

Free radical scavenging activity