metabolites posses antioxidant, anti-inflammatory potentials [23].

The reduction of pure silver ions was confirmed

by UV–vis spectra where the maximum absorbance was seen at 435 nm (Fig. 2). AgNPs stabilized action with
− −
proteins and ascorbic acid which is confirmed by FT-IR spectrum (Fig. 3). The peak at 3423 cm 1, 3006 cm 1, 2926
−1 −1 −1 −1 −1
cm , 1623 cm , 1384 cm , 1033 cm , 710 cm corre- sponds to polyphenols, aromatic C H stretching, C H
stretching, N H bending, C O stretching (alcohols), C N stretching of aliphatic amines, aromatic C H bending. FT-
IR spectrum suggests that the protein present in extract acts as capping agent and ascor- bic acid in extracts
played a role as reducing agent.
SEM images showed aggregation of nanoparticles formed with diameter range from 20 nm to 30 nm (Fig. 4).
TG–DSC analysis, the

Fig. 4. SEM images of synthesized AgNPs in colloidal condition at different nano- metric scales.
 total weight loss observed was about 34.9% suggests that the metal- lic core surrounded by bio molecules. The
◦
unrecompensed residue of silver was around 65.1%. Phase transition was seen at a temper- ature of 951 C
which was also close to the melting point of silver (Fig. 5).
Particle size determination was carried out between 0.1 and 10,000 nm. The average diameter of the
particles was found to be
344.4 and 5272 nm and the peak intensities were found to be 96.04 and 3.6% respectively. A well dispersed
AgNPs were found with respect to intensity.
Compound showed better anti-microbial activity against the growth of pathogen (Fig. 6). The pathogens,
Escherichia coli and
S. aureus were found to be sensitive against the compound. Free radical scavenging
activity of the silver nanoparticles was assessed by DPPH assay. The freshly
prepared DPPH solution exhibited a deep purple colour with a maximum
absorbance at 517 nm