X-ray diffraction analysis

reflections are sharp with good intensity

The crystalline structure of silver
which confirms that the structures of
nanoparticles was confirmed by X-ray
synthesized nanoparticles are well
diffraction pattern. Intense diffraction peaks
crystalline. Our findings match with the
due to AgNPs are clearly observed at
reports suggested.
38.24°, 44.42°, 64.44° and 77.40° (Fig.
Fig. 4. X-ray diffraction pattern of bio-synthesized
4) are
AgNPs of Amphiroa fragilissima
pertaining to the (111), (200), (220) and

(311) planes of Bragg’s reflection based on

the structure of silver nanoparticles. No

reflection peaks related to nitrate ions and

other impurities were observed in this

pattern, which indicates the high purity of the Antibacterial studies

end purity of the end product. In addition, In the present study, the antibacterial activity of

green synthesized AgNPs were tested against B.

the acquired
subtilis, E. coli, S. aureus, K. pneumoniae and

P.aeroginosa with various concentrations (20,40, 60,

80 and 100 µL). The antibacterial activity of

Amphiroa synthesized AgNPs showed the

maximum zone of inhibition for ( S. aureus)

which was 21 mm for 100 µl; 13 mm for

both 20 and 40 µl. The next highest

restriction zone was found for (E. coli) which

was 18 mm for 100 µl followed by 14 mm for

80 µl (Fig. 5 and Table 1).

Table 1. Antibacterial Activity of Silver Nanoparticles Against Clinical Pathogens

Clinical Pathogens Gram +ve Amphiroa+AgN03 (mm)

/Gram –ve 20 µl 40 µl 60 µl 80 µl 100 µl
Bacillus subtilis +ve - 10 - - -
Escherichia coli -ve 11 11 10 14 18
Staphylococcus aureus +ve 13 13 10 11 21

Klebsiella pneumonia -ve 12 12 - 9 11

Pseudomonas aeroginosa -ve 10 11 11 10 13