Control Referenc Method Of Characteristic

Media Function Incubation Criteria

Strains e Media Control Reactions
E. coli
Plate Count Tryptic Quantitative
Productivity 48 H/ 35OC ATCC PR ≥ 0.7 White colonies
Agar Soy Agar Pour Plate
25922
S.
cerevisiae Tryptic Quantitative
Productivity 60 H/ 25OC PR ≥ 0.7 White colonies
Potato ATCC Soy Agar Pour Plate
Dextrose 9763
Agar E. coli
Selectivity 60 H/ 25OC ATCC NA Qualitative Total Inhibition -
25922
S. aureus Black grey
24 – 48 H/ Tryptic Quantitative
Productivity ATCC PR ≥ 0.5 colonies with
35OC Soy Agar Spread Plate
Baird 25923 clear halo
Parker Agar E. coli
Selectivity 24 H/ 35OC ATCC NA Qualitative Total Inhibition NA
25922
Pink to red
E. coli colonies with
Tryptic Quantitative
Productivity 24 H/ 37OC ATCC PR ≥ 0.5 or without
Soy Agar Pour Plate
Violet Red 25922 precipitation
Bile Agar halo
S. aureus
Selectivity 24 H/ 37OC NA- Qualitative Total Inhibition -
ATCC
Brilliant
Turbidity 2+ Gas Gas
Green Bile 24 – 48 H/ E. coli Tryptic Semi
Productivity in 1/3 of Durham Production and
Lactose 35OC ATCC Soy Broth- Quantitative
Tubes Turbidity
Broth
Brilliant
Green Bile 24 – 48 H/ S. aureus
Selectivity - Qualitative No growth -
Lactose 35OC ATCC
Broth
Quantitative Black grey
24 – 48 H/ Tryptic
Productivity . coli Spread PR ≥ 0.5 colonies with
Eosin 35OC Soy Agar
Plate clear halo
Methylene
Blue
Selectivity 24 H/ 35OC NA Qualitative Total Inhibition NA

Media
Quantitative
72 H / L. sake Batch
Productivity Spread PR ≥ 0.5 NA
30OC ATCC already
MYP Agar Plate
validated
72 H / E. coli
Selectivity - Qualitative Total Inhibition -
30OC ATCC

Plate Count E. coli Tryptic Quantitative

Productivity 48 H/ 35OC PR ≥ 0.7 White colonies
Agar ATCC Soy Agar Pour Plate

S.
Tryptic Quantitative
Productivity 60 H/ 25OC cerevisiae PR ≥ 0.7 White colonies
Potato Soy Agar Pour Plate
ATCC
Dextrose
Agar E. coli
Selectivity 60 H/ 25OC - Qualitative Total Inhibition -
ATCC
 Pink to red
O E. coli Tryptic Quantitative colonies with or
Productivity 24 H/ 37 C PR ≥ 0.5
ATCC Soy Agar Pour Plate without
Violet Red precipitation halo
Bile Agar
S. aureus
Selectivity 24 H/ 37OC - Qualitative Total Inhibition -
ATCC
Brilliant
Turbidity 2+
Green Bile 24 – 48 H/ E. coli Semi Gas Production
Productivity - Gas in 1/3 of
Lactose 35OC ATCC Quantitative and Turbidity
Durham Tubes
Broth
Brilliant
Green Bile 24 – 48 H/ S. aureus
Selectivity - Qualitative No growth -
Lactose 35OC ATCC
Broth
Turbidity 2+
24 – 48 H/ E. coli Semi Gas Production
Productivity - Gas in 1/3 of
Lauryl 35OC ATCC Quantitative and Turbidity
Durham Tubes
Sulfate
Broth 24 - 48 H/ S. aureus
Selectivity - Qualitative No growth -
35OC ATCC

Turbidity 2+
24 – 48 H/ E. coli Semi Gas Production
Productivity - Gas in 1/3 of
35OC ATCC Quantitative and Turbidity
Durham Tubes
E. coli Broth
24 - 48 H/ S. aureus
Selectivity - Qualitative No growth -
35OC ATCC

Source:
ISO/ TS 11133-2:2003. Microbiology of food and animal feeding stuffs – Guidelines on preparation and production
of culture media.