SBA-733Plus Manual

SBA-733 Plus

Semi-Automatic Biochemistry Analyzer

Operation Manual

Statement

Sunostik Company has the right to modify the Operation Manual without prior notice.
Sunostik Company has the right to change the technologies without prior notice.
Sunostik Company has the right to revise the specification of the product without prior notice.
Sunostik Company don’t guarantee anything about this document; including the liability for
guarantee that suggested the marketability and compatibility for same special items
Sunostik Company will be responsible for the instrument’s safety and reliability ,but only when
you make sure the following items you has done correctly:
▲Assembly operations, expansion, re-tune, improve and repair must be done by Sunostik
company’s engineer.
▲The related Electrical equipment must be satisfy with the national standards.
▲You must run the instrument under the correct operational guidance.

In the following situation, Sunostik Company will not be responsible for the instrument’s safety
and reliability.
▲The instrument were Disassembled, stretched, re-commissioned by not Sunostik Company’s
engineer.
▲Repair, modify and dismantle the instrument with non-authorized by Sunostik company.
▲The process of Running the instrument is against to the 《Operation Manual》,and caused the
instrument damage.

After-Service

Free service object:

When your instruments need doing free technical service, your instruments must be satisfy with
the Sunostik company warranty service ordinance.

Charge service object:

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1 Your instrument is not satisfied with the Sunostik company warranty service
ordinance.
2 In the following situation, even your instrument is in warranty, we will not doing
free service to you: Damage by the human, un-correctly using, adopt volt was
un-normal, Irresistible natural disasters, replace the parts and consumables are
not allowed by Sunostik or repair by the person that Non-authorized officer of
Sunostik company.

Contact Us

Company name: CHANGCHUN SUNOSTIK MEDICAL TECHNOLOGY CO.,LTD

Address: 733 Kunshan Road, Economy and Technology Development Zone
Changchun City, Jilin Province, China.
Free Tel: 0086 0431 85823168
0086-4008-168-169
0086-800-846-8622
Fax: +86-431-84651234
Website: www.sunostik.com

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Preface

Thank you for your purchase and use our SBA-733 Plus semi-automatic biochemical analyzer.
This operation manual will guide you how to properly run this instrument .
Before using, please carefully read and understand the contents of this operation manual for
operating the equipment properly, After reading, please safekeeping the operation manual and
placed to the right position for easy getting.
Product name：Semi-Automatic Biochemistry Analyzer
Model：SBA-733 Plus
Product registration number： Kyrgyz armed Food Drug Administration (quasi-) character No.
2007, No. 2400064
Product Standard Number：YZB/JI0185-2006
Production license number：Ji Food Drug Administration Xu 20,051,008 Number of firearms
production
Registered Address：733 Kunshan Road, Economy and Technology Development Zone of
Changchun City, Jilin Province, China.
Production address：733 Kunshan Road, Economy and Technology Development Zone of
Changchun City, Jilin Province, China. The operation manual Release Date: August 2009.
Version：1.0.
File Number：SUNO/SOP-733P-01

Intended Use:
SBA-733 Plus semi-automatic biochemical analyzer is used in clinical diagnosis instrument，the
product can be used for routine biochemical test items：Carbohydrates, enzymes, proteins, lipids,
drug abuse, therapeutic drugs testing, as well as emergency and special biochemical tests, specific
proteins, the immune class category and electrolyte testing.

User
The target audience of this manual is for the professional stuff that working in clinical
laboratory. The following people are included:
　 The staff who operating the SBA-733 Plus in the daily working.
　 The staff who doing maintenance of this equipment.
　 The staff who is learning how to operating this equipment.

Content
This operation manual is for the SBA-733 Plus semi-automatic biochemical analyzer. The purpose
of this operation manual is mainly for helping users understand the SBA-733 Plus semi-automatic
biochemical analyzer’s working principle, structure, operational method, routine maintenance,
problem fixing and so on. Please follow this instructions to operate this instrument.

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Sign
Symbol signs and terminology
Based on the different content, we using some symbol signs for marking, please notice each
symbol sign’s meaning correctly when you read thie user manual.
These signs are as follow:

Sign Description

1．Remind the users following the operation manual, otherwise, may cause personal
injury.
2．Remind the users following the operation manual, otherwise, may damage the
system or affect the test results.
3．Explain the important information of operation by step. or other contents needs the
users attention.

Remind the users following the operation manual, otherwise, there is the
dangerous of biological contamination.

Label and screen printing instructions

The meaning of label and screen printing on the SBA-733 Plus Semi-Automatic Biochemistry
Analyzer as below:

Sign Description

CE Logo

In Vitro Diagnostic Equipment

Risk of biological contamination

Risk of electric shock

Manufacturing Date

Product Serial Number

Warning: high-temperature

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Picture
The pictures in this operation manual are only as the description or give a demonstration, not for
other purposes.

Safety Precautions
In order to use this system safely, please read the following safety precautions. Any breach of the
right operate process may destroy the system damage or personal injury.

Warning
If the user does not run the instrument as the operation manual told you, this may
destroy the equipment and affect the test results.

Prevent electric shock

In order to prevent electric shock, please strictly to following precautions as below:

Warning
When the instrument’s power was on, if you found liquid went inside the instrument
or the instrument was leaking by itself, if the liquid into the system or the systems
internal leakage, Please cut off the power supply immediately, and contact Sunstik's
customer service department or distributors. Liquid may cause electric shock and
destroy the system.

Biochemical risk protection

For protecting the biochemistry risk, please following the below precautions:

Risk of biological contamination

Incorrect use of the sample may lead to infection. Do not touch the sample,
mixtures and liquid waste directly use hand, please wear gloves, work clothes to
avoid being infected when operation, if necessary, please wear protective glasses. If
the sample contact with the skin accidentally ,please processing following the user
standard immediately. Some agents have a strong acid or alkali. Please be careful
using , to prevent the hand and close thing to contact the reagents directly .if the
reagents contact with the hands and clothing accidentally, please washing with soap
and water immediately. If the reagent gets in the eyes accidentally ,please washing
with water immediately and consult an ophthalmologist immediately.

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Waste Fluid treatment

In order to prevent the waste fluid cause the environmental pollution and physical harm, please
following below items when dealing with waste fluid.

Risk of biological contamination

Some substances from Reagents, Liquid quality control, Calibration solution ,
Strengthen the cleaning fluid and waste fluid are control by the pollution
regulations and emission standards. Please following the local emission standards
and consultation with the relevant reagent manufacturers or distributors.

Attention Items
In order to operate the equipment correctly and effectively, please pay more attention to the
following items.
System Usage

Warning
This equipment applies to serum, plasma, urine and cerebrospinal fluid. Out of this
scope, please consult SUNOSTIK. Please reference other clinical symptom or other
test results, when estimate the clinical result.

Environment

Attention
Please set the equipment in the appointed environment followed by instruction. Out of
the appointed environment, the test result will be incorrect and broke the system.
If want to change the system, please connect SUNOSTIK service hotline or local
distributor.

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Electromagnetic wave and Interference Prevention

Attention
Please do not put a noise equipment near to the system. Please turn off any
electromagnetic equipments in the room, where the system in. Such as mobile phone,
radio equipment and so on. And do not use any CRT monitor near the system. Noise,
electromagnetic wave can be cause the malfunction and affect the result.

System Use

Attention
（1）Please performance the equipment followed by instruction. Incorrect use will cause
the wrong results; even the system and person will be harmed.
（2）First time, please check the control to confirm the equipment is run.
（3）After turn off the main power 10 seconds, open again. Otherwise, the system will
be protected. If it is, please turn off the main power then turn on again.

System Maintenance
Please maintain the system regularly followed by below notes. Otherwise, lifetime and
characteristics will be affected.

Attention
（1）Please maintain the system followed “System Maintenance” in the instruction.
Incorrect maintenance will cause the wrong results, affect its characteristics and
lifetime, even system and person can be harmed.
（2）The surface will accumulate dust in long time. Use soft cloth to put in the water
and twist it, then rub the surface lightly. Immerge the cloth in to soap liquid if
necessary. Do not immerge the cloth in ethanol or related solvents. At last, use the
dry cloth to clean.Before cleaning, please close the power and pull out the plug.
During cleaning, please prevent water goes into the system, otherwise, system and
person can be harmed.
（3）Do performance the control analysis, after change the main parts, such as source
lamp.

Parameters Set

Attention
Before use this equipment, please program the volumes of sample, reagent, wavelength
and so on, please follow by the instruction sheet and reagent sheet.

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Content
AFTER-SERVICE .........................................................................................................1
PREFACE ......................................................................................................................3
USER ...........................................................................................................................3
CONTENT .....................................................................................................................3
SIGN ............................................................................................................................4
LABEL AND SCREEN PRINTING INSTRUCTIONS ..............................................................4
SAFETY PRECAUTIONS .................................................................................................5
PREVENT ELECTRIC SHOCK ..........................................................................................5
BIOCHEMICAL RISK PROTECTION .................................................................................5
WASTE FLUID TREATMENT ...........................................................................................6
ATTENTION ITEMS .......................................................................................................6
ENVIRONMENT ............................................................................................................6
ELECTROMAGNETIC WAVE AND INTERFERENCE PREVENTION ......................................7
SYSTEM USE ................................................................................................................7
SYSTEM MAINTENANCE ..............................................................................................7
PARAMETERS SET ........................................................................................................7
CHAPTER 1 INSTRUMENT INSTALLATIONS ......................................................10
1.1 UNPACKING .........................................................................................................10
1.2 INSTALLATION ENVIRONMENT..............................................................................11
1.3 TEMPERATURE AND HUMIDITY REQUIREMENT .....................................................12
1.4 POWER SUPPLY REQUIREMENT .............................................................................12
1.5 WASTE LIQUID BOX ..............................................................................................12
CHAPTER 2 EQUIPMENT OVERVIEW ..................................................................13
2.1 PHYSICAL FORM ..................................................................................................13
2.2 BACK FORM .........................................................................................................13
2.3 MEMBRANE KEYBOARD .......................................................................................14
2.4 DISPLAY SCREEN .................................................................................................15
2.5 THERMAL PRINTER ..............................................................................................15
2.6 RS232 INTERFACE ...............................................................................................15
2.7 PIPETTE AND IMBIBITION SWITCH ........................................................................16
2.8 COLORIMETRY SYSTEM .......................................................................................16
CHAPTER 3 SOFTWARE INTRODUCTION ...........................................................18
3.1 SELF-TEST ...........................................................................................................18
3.2 WINDOW DESCRIPTION ........................................................................................19
3.3 PARAMETERS SETTING .........................................................................................21
3.4 APPLICATION MENU.............................................................................................22
CHAPTER 4 PROJECT PROGRAMMING ...............................................................27
4.1 PROGRAMING INTRODUCTION ...........................................................................27

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4.2 BASIC PARAMETER ............................................................................................27

4.3 CALIBRATION PARAMETER ................................................................................29
4.4 QC DATA ...........................................................................................................30
CHAPTER 5 SAMPLE TESTING ..............................................................................31
5.1 MAIN MENU ......................................................................................................31
5.2 END-POINT ........................................................................................................33
5.3 KINETICS METHOD ............................................................................................37
5.4 OTHER METHODOLOGY .....................................................................................38
CHAPTER 6 STATISTIC SYSTEM ...........................................................................39
6.1 INPUT QC PARAMETERS .......................................................................................39
6.2 QC TEST ..............................................................................................................39
6.3 QC VIEW .............................................................................................................39
6.4 PRINT QC CHART ............................................................................................40
CHAPTER 7 MAINTENANCE ..................................................................................41
7.1 REPLACE PRINTING PAPER ....................................................................................41
7.2 REPLACE FUSE .....................................................................................................41
7.3 REPLACE LAMP ....................................................................................................42
7.4 REPLACE PUMP PIPE .............................................................................................43
7.5 DAILY MAINTENANCE .........................................................................................45
7.6 CLEAN FLOW CELL ...............................................................................................46
APPENDIX A COMMON FAULT ANALYSIS AND CLEARANCE......................47
APPENDIX B TECHNICAL PARAMETERS............................................................49
APPENDIX C ACCESSORIES / CONSUMABLES..................................................52

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Chapter 1 instrument installations

Warning
SBA-733 Plus are required to install by SUNOSTIK’s engineer or by the people who
was authorized by Sunostik company.

1.1 Unpacking
On receiving this instrument, check whether the packing is in perfect condition and
that the seals are intact. Open the box and take out its content with care (Figure- 1.1).
Read this chapter carefully before installing the instrument. Do not throw the packing
material away. In case of the re-shipment, use it again to guarantee good transport.

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Attention
To avoid the un-necessary argument, please check each thing in side the box, make sure
everything that wrote on the packing list is prepared.

Packing List

Description Quantity
Main unit 1
Fuse（2A） 4
Power cable（3 cores） 1
User’ Manual 1
Thermal paper（58mm） 1
Wash solution(100ml) 1
Waste bottle 1
Dust cover 1
Ground connection（1.5m） 1
Silicon tube 1
serial port line（1.5m） 1

1.2 Installation environment

◆ Indoor installation only
◆ Table should be flat(gradient less than 1/200) and no vibration
◆ The table at least able to withstand the weight of 80 KG
◆ Well ventilated place
◆ It will be better without less dust
◆ Avoid the direct sunlight
◆ Avoid to put near the source of wind and heat
◆ Non-corrosive and flammable gases；
◆ No noise source and power supply interference
◆ Far from the brush-type motors and the instrument that often switch on and off
◆ Do not close to the issue of electromagnetic devices, such as mobile phones, radio transceivers,
etc.；
◆ Place for working is less than 2000 m altitude

Attention
The working environment should be well ventilated, if necessary, ventilation equipment

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can be used. However, should avoid blowing air directly to the system.

1.3 Temperature and Humidity requirement

Temperature should be between 0℃to 40℃(Fluctuations less than ±2℃/H)
The humidity for system store should be 30% RH ~ 80% RH and also non-condensing。
If the environment is out of this range, please using air condition to cooling.

Warning
If the system is idle for a long time do not use, this may speed up the system’s
mechanical and circuit aging process, so run the instrument on a regular time is required.

1.4 Power supply requirement

◆ 230V～, 50Hz, 3-core power cable, good grounding；
◆ Well-grounded power outlet to provide the necessary power supply。
◆ Should be has at least 3 meters distance between the instrument and the power supply plug。
◆ The current of power supply plug should be more than 1 A
◆ Choose the right fuse

1.5 Waste liquid box

 Choose the right length of the waste pipe that you need.
 Put one end into the waste liquid bottle.
 Put anther end into the waste liquid exit of the equipment. You can see below
picture for details.

Risk of biological contamination

Make sure wear gloves when install the pipe of waste liquid bottle. Put on work clothes
to prevent be contaminated. Put on the protection glassed if necessary

Waste liquid exit

0

废液瓶 Waste liquid bottle

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Chapter 2 Equipment Overview

2.1 Physical Form

① ⑤

②
⑥

③ ④

① printer cover ② incubation bit cover ③ pipette ④ imbibitions key ⑤ liquid crystal
display ⑥ keyboard

2.2 Back Form

① ② ③ ④ ⑤ ⑥

① power switch ② power supply connector ③ RS232 interface ④ fan ⑤ waste

outlet ⑥ peristaltic pump

Attention
The environment should be ventilation to make the emitting system work
normally, if necessary use the ventilation device. But please prevent the air be blew to
the system directly, otherwise the data will be affected.

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2.3 Membrane keyboard

Three parts of the keyboard: numeric, letter and function keys.

Numeric keys: The keys 【0】～【9】and【.】constitute the numeric keyboard. They
serve for inputting and revising the numerical data.
Letter keys: The keys 【A】～【Z】and【-】constitute the letter keyboard. They serve
for inputting and revising the letter parameter information.
Function keys: Perform routine work and activate some functions.
◆ 【Clear】to delete the mistype data.
◆ 【Wash】to washing the flow cell, in order to reduce the cross-contamination. The
peristaltic pump will be high speed rolling when this key is pressed, at the same time
plenty of distilled water will be sipped in to rinse the flow cell, again press the key to
stop the operation. This function is suggested to be proceeded before and after the test
to reduce the cross-contamination, and improve the test accuracy.
◆ 【<】、【>】、【∧】、【∨】to move the bar to change the focus.
◆ 【Esc】to exit the program or back to the main menu.
◆ 【Enter】to write or confirm the information.
◆ 【Absorb】the key is in the left front of the equipment, to run the peristaltic pump
to sip the sample.
◆ 【F1】、
【F2】、 【F4】match with the words which are displayed at the bottom
【F3】、
of LCD separately, different functions in different displays. Blank means no function.

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2.4 Display Screen

Characteristics:
 240×128 dot matrix
 LED backlight design
 Toshiba T6963C controller
 On-chip 32K Byte SRAM

2.5 Thermal Printer

Characteristics:
 384dots/line
 Resolution 8dots/mm
 Max print speed 75mm/s
 Built-in thermistor to check the needle of the printer.
 Built-in sylvatron to check the paper.

2.6 RS232 Interface

Go though the RS-232 interface to connect the equipment with computer. You can
program a better report by using the enclosed CD software. For details, please refer
to the instruction sheet in the enclosed CD. Meanwhile, you can upgrade the software

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through this interface. (no need to open the cover) Conveniently.

2.7 Pipette and Imbibition Switch

When absorb the sample, put the pipette into the bottom of cuvette to prevent
absorbing the bubble during do this action. After pressing the switch, the imbibition
pump will self-start to absorb the sample into the flow cell. Take away the pipette
from the cuvette quickly, when the imbibition device stopped. At this time in order to
improve the test accurate, the air will be absorbed in this device, to reduce the
cross-contamination between two contiguous samples.

Biological Pollutant Risk

When doing the test, please mark sure the gloves and working clothes are wore, if
necessary take on the prevention glasses.

Attention
When checking the sample, please mark sure the pipette is straight to prevent the
result accuracy is affected.

2.8 Colorimetry System

Colorimetric system includes peristaltic pump, quartz flow cell (32ul), optional
system and mechanism gearing, wavelength adjustment system and Peltier .

The system is controlled by CPU, temperature control and wavelength lookup both be
proceeded under the CPU. To be Noted that you’d better take out the silicon tube from
the peristaltic pump when the instrument will not be used in long time to avoid the
tube block.

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System components are as below:

Warning
The colorimetric system is the main part of this equipment, unprofessional
untrained person don’t open it randomly. Otherwise the properties will be affected.

High Temperature Warning

When change the lamp, mark sure the power is off over than 20 minutes, otherwise
your hands must be burned by the high temperature of the lamp.

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Chapter 3 Software Introduction

3.1 Self-test
After open the power switch, (see ① in the picture of the equipment back form
picture), the display is as below:

The self-test will be started 5 seconds later, to test the memory, printer, filter and
sampling circuit separately and display the status real time. The below picture will be
displayed after self-test completely.

Flash Memory：16.5Mbit
RAM 32Kbit
Printer test OK
Filter test OK
Sample Test OK
Lamp Test OK
Test Self OK

The temperature adjustment will be started after the self-test, refer to below picture.
Main menu will be indicated after the temperature adjustment, or you can enter the
main menu by pressing any key directly. At this time, the temperature adjustment is
kept going without any display.

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Temperature adjusting…

20%

Press any key to enter the main menu

Attention
You’d better to start the test after complete the temperature adjustment to make the test
accuracy.

Attentions for Err or Alarm:

"err: #02 Cannot find flash"：Flash is broken or connection is malfunction.
"Warning: #01 Printer has no paper"：No paper in the printer or connection is
malfunction. Works can not be affected by this alarm.
"Warning: #02 Sample test failed"：A/D read failure. Malfunction of pick-board or
the connection is malfunction between pick-board and main-board.
"Warning: #03 Open lamp failure"：Lamp is off or low A/D.

3.2 Window Description

Window Format:
All windows include in this system with the same format, such as main menu(below
picture): There are three parts in the window: title bar up the window to display the
subjects and time, middle is the customer area to indicate the parameter list and
submenu information, status bar down the window to indicate shortcut key and cue
information.

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Main Menu 07/08/01 14:36:20

Sample Test

Main Menu Description:

There are 6 submenus under the main menu; cursor is rest on the “sample test”
position default. You can move the cursor with keys【<】
【>】 【∨】, the related
【∧】
function also will be indicated, at the same time the related functions will be
displayed on status bar. Press【Enter】 to enter the submenu.

Attention
Press the shortcut keys【1】~【6】to enter the submenu directly.(From left to right; from
up to down). Shortcut key “R” means to delete the results; “P” means to print; “T”
means set time; “M” means to A/D; “U” means to update the window; “H” means the
basic information.

The following is the brief introduction for each submenu:

⑴Sample Test: It is the beginning of testing one sample. After programming one item,
press the key to test.
⑵ Absorbance: It is specially ready for testing the absorbance. You can test the
absorbance after enter the menu. The wavelength, temperature, absorption volume and
delay time can be programmed. There will be a hint when you exit the submenu.
⑶ Programming: You can write all the programs of 100 tests under this menu. You
can also revise the program under this menu.
⑷ Parameter setting: There are four parameters can be set under this menu, such as
measure device, sipping volume adjustment, air sipping and language.
⑸ Application menu: There are 6 functions for using, such as patient record, printer
setting, time, system maintenance, software update and help.

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⑹ QC query: Under this menu, you can check the saved QC information for 100 test
items. There are 3 QC type under each item, each QC type can save the QC result 100
days. There are 2 modes for QC indication (QC data list and QC chart), both can be
printed out. You can evaluate the accuracy of the test items through the QC chart, the
instrument also can automatically calculate out the QC data’s average, differentiation
and variance of the certain month.

3.3 Parameters setting

Under the main menu to select parameters setting or directly press the shortcut key
【4】to enter this window, see as below:

Parameter System 07/08/01 14:36:20

Test Device： Flow cell
Absorption Volume Calibration： 100%
Air Volume： 80uL
Language： Chinese

Sample Test

Measure device setting

The instrument can available for flow cell and colorimetric cup, the default device is
flow cell. The samples sipping and draining is controlled by the peristaltic pump
under the flow cell selection, convenience and efficiency; the peristaltic pump will
stop operation under the colorimetric cup selection, the pump push button should be
pressed for confirmation under the manually sample adding. The advantages of
colorimetric cup are highly-transmittance and few cross-contaminations, etc.

Absorption volume calibration

If there is incorrect, you can adjust the parameter to keep the accuracy. This parameter
is direct ratio with the absorption volume, default 100%.

Air sipping setting

This parameter is used for setting the length of air, the default is 80uL. It can be
adjusted base on the pipette length, air can not be sipped into the flow cell is the
condition.

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Attention
The absorption volume calibration and air sipping setting should be correct, incorrect
setting will cause the wrong results.

Language
The operation language is available for many languages. After selection, it works
immediately.

3.4 Application Menu

To select the application menu or directly press the shortcut key【5】to enter this
window under the main menu, see below picture:

Application Menu 07/08/01 14:36:20

Check the record

Application menu instruction

There are 6 submenus under the main menu. You can move the cursor with the
directional keys by pressing the buttons to select different submenus. At the same time
the related functions will be displayed on status bar. Press【Enter】 to enter the
submenu.

The following is the brief introduction for each submenu:

⑴ Patient record: Enter this window to find the details of the patient test result and
test time, to print or delete. See below picture:

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Record 07/08/01 14:36:20

View Record
Print Record
Delete Record

Input Code |

Record query: To input the code No. (Range of code No. is 1~200). Press key
【Enter】to start query.
Comprehensive print: Input two code numbers and press key 【Enter】to print all the
records between this two codes.
Comprehensive delete: Input two code numbers and press key 【Enter】to delete all
the records between this two codes.

⑵ Print setting: Enter this submenu to set the print parameter, operate the printer and
check the status of the printer, see below picture:

System parameter 07/08/01 14:36:20

Lab Name： SUNOSTIK
Auto Print： On
Print Style： Standard
Print State： Standby

Test Paper

Lab Name: The default is SUNOSTIK, it can be adjusted according to the customer
request, and the name will be printed in the test report. (It is workable with the
standard print type).
Auto-print: The test result can be auto-printed out after complete the test if this
function is in application.
Print Style: There are two print type is optional, default is standard type, there is

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blank between the 2 test results to make sure all the content can be indicated out of
the paper outlet. You can select economy type to save the paper, but the result content
will be compactness.。
Print Status: The item is for read only to indicate the printer status.
Self-test: The shortcut key【F1】is used for testing the paper and checking the printer.
Paper Moving: The shortcut key【F2】is used for moving paper, this function can be
acted when the printer is installed. Repress it to stop the moving.
⑶ Time System: Using the keys【<】、
【>】to move the cursor to revise the date and
time after enter this submenu. See below picture:

Time System 07/08/01 14:36:20

07/08/01 14:36:20

Attention
The time system is very important for working normally, incorrect setting will
mistake the test result and QC data, please take it in serious.

⑷ System Maintenance: Enter in this submenu to check the A/D value in certain
wavelength. See below picture: The default filter is 340nm; A/D value is the last
number. Press key【Enter】to bounce out the wavelength list, you must select a
wavelength to start the sampling, and keep the A/D value continuous and in real
time.

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System Maintenance 07/08/01 14:36:20

Filter： 340
A/D value： 167.1452

⑸ Software Update: You can follow the below picture to apply the latest software to
upgrade the functions. See below picture:

Update 07/08/01 14:36:20

Connecting pc through RS232

Please restart the power for running the new procedures after update.

Download Guide：
Use the matched connect line to connect the computer through RS-232 interface（Rear
view③postion），Open the updated software that we provide to download and
update .Software interface as below:

① ⑧
⑦
⑥

③ 25 ④
⑨
 SBA-733Plus Manual

Help:
③⑤⑥⑦ position : Set up parameters according to the map,⑧ position :According to
the port NO that contacting by equipment and computer for confirmation. After set up
these parameters，Click ① to select the program file ,the extension of the file is .hex，
Such as SBA733.hex。Open the equipment and click ④。After a few seconds,，There
is presentation on ⑨ that show the connecting well or not ,if display "Reading of
PART ID Success" ,it means connect successful，please click ② to download the file
at this time .At the same time there is a blue progress bar showing on ⑨.when the
progress bar run to the right side .download completely .If display "Reading of PART
ID Failed"，that means connection failure .please check the line connection carefully,
Whether the line contact with a solid ,to connect again after the check.

Attention
Please shut off the power of the equipment before download the Software ,use the
matched connect line to connect the computer and equipment. It may burn out the
equipment when connection with the electricity.

⑹ Help：Under this interface you can find the basic information of the equipment and
the contact information of our company, see as below.

Help 07/08/01 14:36:20

Model：SBA-733
NO：SUNO070801001
Vender：Sunostik Medical Technology Co .,Ltd
Version：1.0
TEL：4008-168-169

www.sunostik.com

Please contact with us freely if you have any questions.

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 SBA-733Plus Manual

Chapter 4 Project Programming

4.1 Programming Introduction

Please select the application menu under the main menu or directly press the shortcut
key【3】 to enter into project selection interface, input the item No and press
【Enter】item enter into edit interface, see as below:

ALT 07/08/01 14:36:20

Item name： ALT
Test Mode： Kinetics
Main wavelength： 340
Vice-wavelength： 700
Test temperature： 37

TEST PRINT End page Next Page

Press【<】、
【>】key to move the cursor to switch the focus parameter；Press the
shortcut key【F1】、
【F2】、
【F3】、
【F4】 to achieve the function that opposite on the
status bar ;Press【Enter】key to edit the focus parameter ,at this time ,the cursor is
moved to the editing window or the sub-window ;you can use【Clear】key to delete the
mistake during the editing;；After the editing ,press【Esc】key to exit the submenu and
give up the content which is edited . press【Enter】key to confirm and exit the editing
window or the sub-window, back to the above interface.

4.2 Basic Parameter

Item Name：
Input the English name of the item with the form of edit-box，All the test items of this
equipment is open that you can organize the project scheduling according to your
personal habits .For example, Installed the frequently used items in front ,installed the
related items next to the adjacent number. In order to improve your work efficiency.
Test Mode:
Edit with the form of sub-window, the method include end-point, kinetics, fixed time,
differential and multi-standard, etc., different test method with different data list for
comparing, Does not display the extra parameters , so edit any of these methods are

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 SBA-733Plus Manual

very simple and clear.

Decimal digits:
Edit with the form of edit-window, range: 0 – 4，default is 2, This parameter is used to
trim the number of the decimal digits of the test result, only for controlling the only
measurement results display and print, and will not really change the results of
precision ,so there is no rounding calculation and maintain the original data accuracy.
For example: if you input “0” in the decimal digits，the test result showed that “32”
(assuming the true results of 32.6789），when you modify this parameter is 4, the
results can still be a true showing of 32.6789.
Main Wavelength:
Edit with the form of sub-window ,There are 7 wavelength for your selection :340、
405、505、546、578、620、670.please select the wavelength according to the user’s
manual of the reagent.
Vice Wavelength：
Edit with the form of sub-window, There are 7 wavelength for your selection:340、
405、505、546、578、620、670.please follow the user’s manual of the reagent to select
the wavelength correctly when the reagent requires to use of dual-wavelength for
testing. If the parameter is selected as "×" or the same with main wavelength, then the
vice-wavelength not be used.
Test temperature：
Edit with the form of sub-window，there are three items for your selection:25、30、
37.
please select this parameter according to the user’s manual of the reagent correctly.
Absorbance Volume:
Edit with the form of edit-window,Range:200 – 1500 uL，programmed by 0.1
step.Absorbance volume is too few will affect the test precision ,absorbance volume is
too much will waste the reagent .please setup consideration
Delay Time:
Edit with the form of edit-window,Range:3 – 999 S，please set up this parameter
according to the user’s manual of the reagent correctly.
Incubate Time:
Edit with the form of edit-window,Range:1 – 999 S, please set up this parameter
according to the user’s manual of the reagent correctly
Measure Time:
Edit with the form of edit-window,Range:30 – 999 S, please set up this parameter
according to the user’s manual of the reagent correctly.
Thickness Unit：

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 SBA-733Plus Manual

Edit with the form of sub-window .there are 13 ordinary Units for selection. please set
up this parameter according to the user’s manual of the reagent correctly.
Reference Upper Limit：
Edit with the form of edit-window, This is the upper limit of the reference
range .please set up this parameter according to the user’s manual of the reagent
correctly.
Reference lower Limit：
Edit with the form of edit-window, This is the lower limit of the reference
range .please set up this parameter according to the user’s manual of the reagent
correctly
Correction coefficient:
Edit with the form of edit-window. When the test results is generally low or too high,
you can use this parameter to amend the results.

Attention
Default number is 1，To setting this coefficient should be according to the huge
statistic data, it should be applied in serious.

4.3 Calibration parameter

Calibration Mode:
Edit with the form of sub-window,there are two modes for selection:STANDARD and
FACTOR. If you select standard ,the standard liquid should be used during the test.
The equipment will calculate the factor and saved it for the future automatically. If
you select factor, you can apply the factor for the test directly to save the standard
liquid, there is no need to set the calibration. If the test mode is Kinetics, the
calibration will be inefficacy and hided automatically.
Factor Value:
If the calibration mode is Factor, The factor value must be input for calculation. If the
calibration mode is standard, the factor value will be inefficacy and hided
automatically.
Standard quantity:
If the test method is multi-standard, that need to set the quantity of the standard liquid,
other test method , this parameter will be inefficacy and hided automatically.

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 SBA-733Plus Manual

1. Standard Thickness:
If the calibration mode is standard, the standard thickness should be input for
calculation. If the calibration is Factor, this parameter will inefficacy and hided
automatically. If the test method is Kinetics, this parameter will be inefficacy and
hided automatically. The standard thickness will be show X when the test method is
multi-standard.
2. Standard value X:
The standard value X should be input under the multi-standard test method（each
thickness of the standard liquid），The maximum range is 2-6 , it is according to the set
of standard quantity. Under the non multi-standard method, this parameter will be
inefficacy and hided automatically.

Attention
The setting of the standard value has to be followed from small value to
big value , or it will cause the mistake result.

4.4 QC data
Target H、Target M、Target L:
Edit with the form of edit-window, separate as QC high value, QC middle value, QC
low value. please set up this parameter according to the user’s manual of the reagent
correctly
Standard difference H、Standard difference M、Standard difference L:
Edit with the form of edit-window, separate as the standard difference for QC high value, middle
value and low value, to be applied for plot of the QC chart. please set up this parameter according
to the user’s manual of the reagent correctly

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 SBA-733Plus Manual

Chapter 5 Sample Testing

5.1 Main Menu

MENU 07/08/01 14:36:20

Sample Test
Press 【Enter】or shortcut key【1】to enter the following menu on above picture,
namely the “sample test”, see below picture:

ITEM 07/08/01
01 AST 06 CL-,MB
02 ALT 07 ALP
03 a-HBDH 08 α-AMYS
04 LDH 09 γ-GT
05 CK 10 GLU
Input：| END/P NEXT/P

Please input the test item No under this menu. if the item is not exist on the interface ,
press shortcut key【F3】
【F4】，according to the next page indication on the screen to
search the item which you need, total are 10 pages cycling, After input the No, press
【Enter】to enter the baseline test interface.

Attention
For example: If you want to test “AST”,please press the key【1】and 【ENTER】enter
to the baseline test interface ,see as below.

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 SBA-733Plus Manual

AST 07/08/01 14:36:20

Baseline

Absorb the water

The above picture is in case of the measure device is flow cell, and the information
indicated in the status bar is “Absorb the distilled water”，please prepare the distilled
water and press the Absorbance button, the peristaltic pump start to working. The
indicated content will be “absorbing…”. After absorb the distilled water, the status bar
will display “withdraw the cuvette ” with one beep. Please take out the pipette from
the cuvette at this time, and the analyzer will absorb in the certain air (the air
absorbance item is included in the system parameter) to separate the different liquid,
In order to avoid the cross-contamination. If the measure device select the
“Colorimetric cup”, The above status bar will show “add the distilled water ”.You
should put the colorimetric cup full filled with the distilled water and press the button.
There is no absorb procedure when use colorimetric cup the mentioned as above. It is
the same for adding the sample, we will not mention again. The delay time will be
come out after finish the absorbance , in order to level off the distilled water,, and the
status bar will show “in delay 00X”, the unit for X is the second and count
down .The status bar will be show “in A/D value reading” after the delay time .If the
baseline read failed ,it will show below picture.

AST 07/08/01 14:36:20

Read baseline failure!！

Mean = … CV% = …

Absorb the distilled water

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 SBA-733Plus Manual

The reason for baseline reading fail is as below:

1. If CV%（coefficient of variation）exceed 5%，it means the set-up time of the
analyzer is too short and the analyzer is not in steady, please wait a moment and
again try. Or it is caused by the aging of the peristaltic pump，the liquid is in fluid
status, please replace the peristaltic pump and try again.
2. If Mean（Average value）under 150，maybe caused by the bad connection of the
flow cell or the colorimetric cup，or there are some air bladder in the distilled
water, please deal with it and again try.
If read the baseline successful, it will enter into the different interface base on the
selected test item; the following will introduce of the procedure for each test method.

5.2 End-point
The interface of end-point testing as below（take GLU for example，only for your
reference）.

GLU 07/08/01 14:36:20

Parameter

Blank = 0

Factor = 1746

Black Standard Sample QC

The default parameter will be the last test data of blank and factor. you can directly to
start the sample or QC test if the indicated data is in your requirement. If the
parameter does not match your inquire or you want to reset the parameter for
increasing the test result .If not, you can press the shortcut key according to the
content of the status bar, reread, blank and standard. below is the instruction for the
four interfaces: Press【F1】to enter the test interface for blank test, see below picture:

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 SBA-733Plus Manual

GLU 07/08/01 14:36:20

Blank

Blank =
Factor =

Absorb the blank liquid

The work procedure is the same with absorbing the distilled water , we won’t mention
again, it will return the up menu after reading blank, the data in the system is updated,
if the result is not fit for you, you can retest it follow the same procedure .retest the
blank. Press【F2】to enter into the interface of standard, see as below:

GLU 07/08/01 14:36:20

Standard

Standard =
Factor =

Absorb the standard liquid

The work procedure is same with reading the baseline procedure, we won’t mention
again. It will return the up menu after the reading standard, the standard and factor
parameter is updated at this time, now it finish setting the standard. If the result is not
fit for you, you can retest it follow the same procedure. Reset the standard. Press【F3】
to enter into the interface of sample test, see as below:

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 SBA-733Plus Manual

GLU 07/08/01 14:36:20

Sample ID：001|

Absorbance =
Result=

Absorb the sample

The default patient No. is 001，the No. can be edited when the cursor in flash. Press
Key 【Clear】to delete, and use numeric key to edit. The range of the No. is 1--999
（the first 200 patient the test result can be saved automatically）. Please press the
button to absorb the sample. The procedure is same as absorbing the distilled water .
we won’t mention again. It will come into the below window after absorb the
sample , the result will be sent to the computer through RS-232. If the auto-print
function is on turn(this function is edited in the print editing), the test report will be
automatically printed out .Otherwise, please press【F4】to print the test report.

GLU 07/08/01 14:36:20

Sample ID：001|

Absorbance = 0.5547
Test Result = 30.22

Next Retest Reread Print

You can select the retest or reread function if you are not satisfied with this result.
Retest: Does not to change the patient No. and re-process the above procedure .need
to absorb the sample again
Re-read: Does not to change the patient No, and no need to absorb the sample again,
reread the current sample
If you want to proceed the other sample test under the same item, please press
shortcut key【F1】to enter the test interface and the patient No. will be add 1

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automatically . it will test the next sample with reprocess the above procedure.
The next test also can be followed with the same procedure. You also can directly
press the button to start the new sample test without press【F1】，the patient No. will be
added 1 automatically and enter to the procedure of absorbance and testing, in order
to simplify the operation.

Attention
Please press 【Wash】 when the test is finished ,to avoid the
cross-contamination .Press【Wash】again to stop washing.

Press 【F4】 to enter the interface of QC control ,see as below.

GLU 07/08/01 14:36:20

QC H M L

Absorbance =
Result =

Absorb the QC liquid

The QC type is default as the normal QC value, see above picture, the cursor is on the
M point. If you’d like to do the abnormal value, use【<】、
【>】to move the cursor. The
procedure of absorbing and reading is the same as the reading baseline , we won’t
mention again, , it will come into the below window after reading the QC:

GLU 07/08/01 14:36:20

QC

Absorbance = 0.5547
Result = 30.22

Blank Stand Sample QC

If you have some doubt for the above QC result or you’d like to do the other type of

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QC, press【F4】to reprocess the above procedure. Under the same item , type and date,
only the latest QC result will be saved.

5.3 Kinetics Method

The interface of kinetics testing as below（take AST for example，only for your
reference）.

AST 07/08/01 14:36:20

Parameter

Blank = 0
Factor = 1746

Black Sample QC
The blank data that display in the data list is default as the latest test result, you can
directly start the sample test or QC if the blank data is in your requirement. If the
parameter is no fit for this test or want to retest the blank in order to increase the test
accuracy. please re-read the blank data base on the indication on the status bar.
Compare with End-point, it is no need to make the standard .so it is no standard
optional .we are only to introduce the interface of sample test, the other procedure
please refer to the End-point test method.
Press 【F3】to inter the interface of sample test .see as below

AST 07/08/01 14:36:20

ID：001|
Absorb：
0
Factor：1746

Absorb the sample

On the left side of workspace is an coordinate system which put time on X-axis and
absorbance on Y-axis in order to draw a reactive curve .The control no. on the left side
of workspace defaults to 1,and could be edited freely ,the same as end-point

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method .Absorbance could real-time display its present value in the whole testing
process of sample .Factor is an assigned numerical value under control of program.
Please press aspirate liquid key to inhale the sample ,then it will enter the phases of
hatching and testing samples. The following window will come out after test.

AST 07/08/01 14:36:20

ID：001|
d A/m：
0.0207
Result：36.21

Next Retest Reread print

The curve in coordinate system is a calculated linear regression which reflects
sample’s changing trend and the starting absorbance value .If you would like to print、
re-read、re-test or test other samples ,please see end point method.

5.4 Other methodology

 About the operating interface and operation method of two points ,please see
end point method.
 Differential belongs to the expansion of end point, about the operating
interface and operation method ,please see end point method.
 Multi-standard also belongs to the expansion of end point, about the
operating interface and operation method ,please see end point method.

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Chapter 6 Statistic System

6.1 Input QC parameters

In order to ensure authenticity and validity of QC data ,QC test is programmed and
tested with sample test at the same time .About editing of QC parameters, please see
item programming.

6.2 QC Test
In order to ensure authenticity and validity of QC data ,QC test is programmed and
tested with sample test at the same time .About editing of QC parameters, please see
item programming.

6.3 QC View
Press QC View under main menu or directly press shortcut key【6】to enter into
application control menu ,please see following picture:

ALT 07/08/01 14:36:20

Input month： |

High Mediam Low

Input your demanded month ,press【F1】、【F2】、【F3】to choose QC type.

The following is list of QC data:

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 SBA-733Plus Manual

ALT 07/08/01 14:36:20

32.18 +1SD 07/07/01
28.98 +1SD 07/07/02
36.97 +2SD 07/07/03
36.01 +2SD 07/07/04
26.11 - 1SD 07/07/05

Chart Print Back Next

Shortcut keys 【F1】、

【F2】、 【F4】separately stand for different functions
【F3】、 on
above status bar. Press shortcut key【F1】will draw out following QC chart.

ALT 07/08/01 14:36:20

3sd X
2sd 32.2218
1sd SD
X 1.0548
-1sd
-2sd CV%
-3sd 1.0256
1 10 20 30

Data Print Back Next

6.4 PRINT QC CHART

In above two QC interface ,press shortcut key【F2】to print QC report and QC chart
separately.

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Chapter 7 Maintenance

7.1 Replace printing paper

When installing printing paper, please cut the end of paper to a correct shape
according to following picture. Extend the printing paper to feeder of the printer.

Incorre correct

When the instrument is on working(when power is turned on ,it the printer processing
power ),at this time, send printing paper to the feeder under the paper delivery
roll ,press【paper feed】,the printing paper will come out automatically.

7.2 Replace fuse

Fuse ① is on the rear of instrument ,it was installed together with power button,
Rating current is 6A.

1
When replacing fuse, firstly twist off the cap ,remove old fuse and install a new
one equipped together with instrument.

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Warning
If you replaced a new fuse and turned on power ,the fuse was burned at
Once .Under this situation ,please don’t change a higher current fuse any
More ,turn off the instrument and get in touch with our maintenance
engineer at once.

7.3 Replace lamp

The instrument uses a 6V/10W halogen lamp ,life span is about 2000 hours ,because
the lamp is in high usage frequency , after a period of usage the lamp will be not
bright enough or can’t work any more ,at such time ,you need to replace a new lamp.

First, twist off the cap, loosen two inner hexangular set screws（2）be used to fix
component parts of lamp base （4） with an inner hexagon spanner，remove the
component parts and remove halogen lamp （1）from lamp base（3）.Install a new
halogen lamp on the lamp base ,and install the component parts on the lamp
holder ,fasten the screws with inner hexagon spanner .Please see the following
pictures to check detailed installing position.

3
4

1 2

High temperature warning

When replacing lamp ,you must wait 20 minutes after turning off the power ,or your
hands may be scalded by high temperature comes from the surface of lamp.

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Ajust light source：

When you need to replace lamp, loosen fixed screws under the instrument to open
upper case .Please pay more attentions when removing upper case to prevent electric
shock .To place upper case down from the right side(facing the instrument direction).
Loosen hexangular set screws with inner hexagon spanner .Install the new lamp as
original way .Before fixing lamp base ,assure the strongest light of lamp is just in the
centre position .You must assure filament is perpendicular to bottom board .After
proper adjustments ,please fix lamp base with inner hexagon spanner.

Adjustment and detection:

After fixing lamp ,please monitor A/D Value under
maintenance window when standard filter is 340nm, the A/D value should be larger
than 120nm at least ,if this value is too small ,please readjust position of lamp .please
see below operating interface:

System maintenance 07/08/01 14:36:20

Filter： 340
A/D value: 189.1452

7.4 Replace pump pipe

 Peristaltic pump located in the rear of instrument ,If you find the instrument don’t
aspirate liquid or the volume disagree with pre-set value(software setting didn’t
work),please remove peristaltic pump and check the reason .One possible reason
is the pump pipe blocks or a long time using and wearing happens to peristaltic
pump self ,both of these make an increasing interspace between shaft and three
pulleys ,at such situation ,you need to replace peristaltic pump or pump pipe.
 The way to remove peristaltic pump: first ,disconnect the pump from
pipeline ,then press the pipe joint outside pump with your left hand ,at the same
time ,pull the pump pipe out with hard strength.

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 After disconnecting the pump ,press the clips on both sides of peristaltic pump ,at
the same time, pull the pump pipe out with hard strength.
 Please take the following steps for your reference on how to exchange pump pipe.

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7.5 Daily Maintenance

 Never use cleaning agent or abrasive cleansers, use clean water or fabric dipped
with neutral soap .
 In case of any reagent or corrosive substances were splashed on instrument,
clean it at once with damp cloth and clean water.
 It is necessary to clean liquid circuit on correct way after finishing one group of
tests and daily job .Clean with plenty of distilled water after finishing one group
of tests.
 Clean liquid circuit with equipped cleaning agent everyday after work,clean tube
with plenty of distilled water circular to empty liquid circuit.
 If the end of suction tube is broken ,you could cut down several millimeters
vertically and clean the cut part.
 If the tube is seriously run of date ,please change a new one and use equipped
instrument components
 If long time don’t use the instrument ,please empty liquid circuit, then pull out
waster liquid tube and store it suitable.
 Clean surface of instrument with non-abrasive cleansers every month.
 Wipe off dust fell outside the fan every month.
 Clean the tube and flow cell after finished tests everyday to avoid dirty
precipitate.
 Don’t pull flow cell out of instrument unless cleaning the flow cell .Do pull and
plug the bolt slightly .also pay attentions to placing position of flow cell ,never
install it on oppositely direction and lead to damage.
 A canonical operation way could extend service life of instrument .Never press
soft keyboards with sharp nail on which way the keyboard could be easily
broken .You should press it with finger belly .
 When installing printing paper ,if a bit of paper was left in printer ,please don’t
pull it out forcibly，because such way could easily damage the printer .The right
way is press【Paper】key to send out paper.

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Warning
Most maintenance were carried on under power off state ,so please maintain it after
turning off power for safety.Never spray any liquid like reagent to instrument or
electronic accessories.

Biology contamination danger

Do wear gloves and cloth to prevent from being infected ,wear protective glasses if
necessary.

7.6 Clean flow cell

Both misusing of flow cell and drawing it out frequently all may cause a cut or a
permanent damage on surface of the flow cell .Dust on the surface may decline
accuracy of measurement .So please don’t draw flow cell out in normal circumstances.
Wipe flow cell with lens paper instead of hard material to avoid the cut of surface.Pay
special attention to installing direction after cleaning to avoid an unnecessary damage
due to an opposite installation .Please see following pictures for your reference:

The side marked with arrow faces front operating interface.

Attention
When wiping flow cell ,do choose the soft material like lens paper to prevent cutting
light-hole which will affect the Visible Light Transmission .At the same time ,pay
attentions to installing direction.

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Appendix A Common Fault Analysis and Clearance

Warning:
When settling troubles ,please handle it with great care ,check whether deal it after
power is turned off

Examples of
Reasons analysis Eliminate methods
trouble
1 power cable is broken or on a poor
1 Replace or inspect the power cable
Power light don’t contact
2 Replace the fuse
work 2 Fuse blows out
3 Replace the switch
3 Power light fails
1 Have not pluses negative voltage of 1 Inspect the negative voltage of LCD
LCD 2 Check if flat cable and LCD are in good
LCD don’t work
2 Flat cable is off contacts
3 LCD is broken 3 Replace the LCD
1 Control circuit broke down
1 Check the control circuit
Peristaltic pump 2 Aspirating liquid switch is broken or
2 Adjust or replace injector port
don’t work or don’t on a poor contact
3 Replace the peristaltic pump
aspirate liquid 3 Peristaltic pump is broken
4 Replace the pump pipe
4 Inside pump pipe blocks.
1 Clean the flow cell repeatedly with distilled
water and check autosampler system
2 Check whether the reagent is out of date.
1 There are bubbles or dirt in flow cell
3 Check whether your selected filter confirms
2 Reagent turns bad
to test requests.
3 Selected filter don’t confirm to this
4 inspect the whole operating process and
test.
Test abnormity test again.
4 Incorrect operation or large error
5 Check the halogen lamp and test the voltage
5 Halogen lamp is broken or with an
of it(6V more or less)
insufficient light source
6 Check whether couveuse’s temperature
6 Couveuse’s temperature is inaccurate
confirms to the requested test
temperature ,if the error is big ,adjust
temperature to requested test temperature.

1 Control panel is broken 1 Check the control panel and replace it.
Keyboards don’t
2 Fast booting and Shutting down lead 2 Reboot the instrument (turn power off and
work
system halted then reboot it)

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1 Check Signal Connection(Printer cable)

1 The instrument is not online.
2 Check whether voltage is 5V
2 Check printer’s power.
3 Turn off the power and re-operate
3 Inadequate operation
Printer don’t work 4 Check a correct installation to make sure
4 Wrong installation way of print paper
or can’t print any thermal face upwards
5 Printing head broke down
word 5 Replace the printing head
6 Internal wiring or Semiconductor
6 Overhaul the internal wiring and
Component was broken
semiconductor component
7 Check locking bar’s position
7 It should be in locked state
1 Check whether the voltage fluctuation of
power is so large
1 Check whether light source is stable 2 Clean the flow cell with cleaning fluid
2 Whether flow cell is contaminated equipped together with instrument.
Bad Repeatability 3 Liquid absorption leads to some
3 Calibrate the liquid absorption refers to
and unstable reading bubbles.
chapter “Temperature and liquid
4 Long staged air leads to suction some
absorption”
bubbles
4 Use sample instead of air when aspirate
staged air.

During the test,when 1 Check whether there is a clock item under

press keys of “delay 1 Malfunction of chip in clock or main menu ,if not, enter into “service
time”and “test battery is exhausted menu” and reset it.
time”,time stand still

2 temperature sensor is broken or

circuits used for transmit signal are
1 Replace the
broken.
2 Replace the sensor or circuit used for
Incubation slot don’t 3 Something happens to temperature
transmit signal
heat or overheat controlling circuit(Open the cover and
3 Replace the broken component or replace
see whether instruction light still
the whole temperature controlling circuit.
work)

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Appendix B Technical Parameters

 Light source:6V/10W imported quartz halogen lamp.

 Standard filter :including 7 filter, 40nm、405nm、505nm、546nm、578nm、
620nm、670nm.
 Linearity range: 0～3.000O.D
 Cross contamination rate: ≤1%
 Blank :the instrument could automatic store the blank value when testing.
 Factor :the instrument could auto recalculate the factor when without
modifying any program parameter.
 Interface :there are 48 keys in main keyboard used for inputting figures、
letters or execute other quick function;240X128 dot-matrix LCD
 New functions on ungrading and updating system(IAP function)
The instrument could update program at any time through RS-232 serial
port with equipped serial port cable.
This function provide a good support to users on using the latest software
version.
 memory unit(memory system)
 Main program was saved in inside of CPU.
 Programming contents and results were saved in Super Flash Memory
Memory unit could save Parameters under 100 test.including :item
name、test method、wavelength choice、temperature、 unit、reference
range、delay time、test time、aspiration volume、decimal digits、
standard deviation、QC target value .You could edit and modify above
items freely.
 Memory unit could save QC information under 100 items ,there are
three kinds of QC type under every item ,every QC type could save
100 results.
 Parameters like blank and factor under every item could be auto
saved ,the saved factor could be displayed and modified under
program interface .You could use these parameters directly for next
time.
 Measuring Mode:
 End point :you can calculate with factors directly ,this method could
save reagent and standard liquid.
 Kinetics :this method could real-time display absorbance of

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sample ,auto display response curve After the immunoreaction .

 Tow points :used to calculate changing parameter of absorbance
between two intervals.
 Differential :you can use reagent blank(6 tubes)or not use reagent
blank(4 tubes)
 Multi-standard :used for nonlinear sample tests.
 QC
 You could set parameters(QC target value) of three QC types when
editing programs.
 The 100 test items all have function of QC.
 You could view and print QC chart and QC parameters(average value，
standard deviation, coefficient of variation )
 Delay time: Programmable Object Select （0～999s）.
 Test time: Programmable Object Select （0～999s）,test time of rate assay
and two points are 30s~999s.
 Quartz windowed flow cell
 Capacity is 32ul,diameter is 1cm.
 Special design on shape could decline crossed contamination rate and
easy maintenance.
 Min suction volume is 300uL.
 Temperature control
 Optional temperatures are : 25℃、30℃、 37℃
 Precision: ±0.2℃
 Accuracy: ±0.1
 Aspiration system
 Imported peristaltic pump was installed in instrument.
 This aspiration system was installed in the front-end ,you could
replace peristaltic pump without opening the cover ,this design is easy
for maintenance .Aspiration volume was controlled by program，users
could modify and adjust this volume by themselves.
 Min aspiration volume is 300uL,Max aspiration volume is 1400uL，
the volume could be continuously Adjusted.
 Power source :
 Voltage：230V～
 Frequency：50Hz
 Power：MAX 70VA
 environmental condition:

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 Working temperature：10～35℃
 Environment humidity：≤80%
 Volume： 430（length）×320（width）×220（height）
 Weight： 10 Kg

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Appendix C Accessories / Consumables

Please use accessories made or recommended by SANOSTIK to ensure personal

safety and a stable system performance. If necessary，please get in touch with
aftermarket department of SUNOSTIK or rural distributor.

Name of parts Specifications Position remarks

lamp 6V/10W light path ≥2000 hours

peristaltic pipe Φ3*Φ2*160m on back panel of instrument damaged

flow cell optical path 1cm incubate position damaged

pump DC12V on back panel of instrument damaged

fuse 6A/220V φ5×20 on back panel of instrument damaged

thermal paper printer Consumables

cleaning fluid Consumables

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