Object 1: Introduction To Standard Laboratory Conditions and Diet For Mice & Rats

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Object 1: Introduction to standard laboratory conditions and diet for mice &

rats
Area

Clean & hygienic

Light: Dark cycle

12 hour light:12 hour dark cycle

Temperature

25±2 °C

Relative humidity

55±15%

Cages

Plastic, non-toxic, non-absorbable material, autoclave able, ventilated, flat bottom. Cage should
be cleaned every 3 days

Bedding

Wood chips, not less than 2 cm depth, bedding should be changed every 3 days

Diet

Standard chow diet, drinking tap water

Lifespan 2.5-3.5 years

Adult weight Males 300-500g, Females 250-300g

Birth weight 5-6g

Heart rate 330-480 beats per minute

Respiratory rate 85 breaths per minute

Body temperature 35.9-37.5ºC

Blood volume 50-70 ml/kg

Urine volume 3.3 ml/100g bwt/day

Pregnancy or gestation period: 21-23 days; upto 12 pups in one delivery


Standard chow diet

Bedding
Lifespan 2.5-3.5 years
Adult weight Males 300-500g, Females 250-300g
Birth weight 5-6g
Heart rate 330-480 beats per minute
Respiratory rate 85 breaths per minute
Body temperature 35.9-37.5ºC
Blood volume 50-70 ml/kg
Urine volume 3.3 ml/100g bwt/day
Allergens Dander, urinary protein
Pregnancy or gestation period: 21-23 days; upto 12 pups in one delivery

Weight of pup at the time of delivery: 5-6 gm

A rat of 250 g will contain around 15 ml of blood

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