Practical # 10

Determination of Bleeding Time

Requirements:
➢ Lancet, stop watch, Whatman Filter paper, pencil, cotton swap and spirit
Learning Objective:
➢ To determine bleeding time
Introduction:
A bleeding time test determines how quickly your blood
Discovery:
clots to stop bleeding. The test involves making small
punctures in your skin. The test is a basic assessment of The bleeding time test was first
described by Duke in 1912 and
how well your blood platelets work to form clots. modified by Ivy in 1941.
Platelets are tiny cell fragments that circulate in your
blood. They’re the first cells to react to a blood vessel
injury. They seal off the wound to prevent more blood from escaping.
Normal Value:
➢ 1-3 minutes (Duke’s method)
Procedure:
• Disinfect finger with alcoholic swab
• Prick it with sterilize lancet
• Start the stop watch
• Wipe out the blood with filter paper
• After 30 sec wipe out the finger
• And repeat the process until blood spot become
fade
• Calculate the time at the end of experiment
Precautions
• Avoid air bubbles in the capillary tube
• Keep ends of the tube open when filling with blood
Laboratory Questions:

1. Define bleeding time and enumerate clotting factors?

2. What is the role of clotting factor in the blood clotting?
3. What are the clinical conditions in which bleeding time is prolonged?
 Observation and Results

Student Name: syeda mehak hassan_______________

Date12/10/2020:_______________

Teacher Name: _______________

Activity:

Write your estimated bleeding time after performing the whole protocol and also paste the
filter paper here with proper labaelling.
 Signature:______________________

Feedback:______________________

Write to the point Answers of Laboratory Questions:

Answer no 1.

The time elapses between the puncture of the skin and the stoppage of the blood
oozing out

I Fibrinogen

II Prothrombin

III Tissue factor or thromboplastin

IV Calcium

V Proaccelerin (Labile factor)

VII Proconvertin (Stable factor)

VIII Antihaemophilic factor A,

Antihaemophilic globulin
 IX Antihaemophilic factor B,

Plasma thromboplastin component,

Christmas factor

X Stuart-Prower factor

XI Plasma thromboplastin antecedent,

Haemophilia C,

Rosenthal syndrome

XII Hageman factor

Answer no 2:

Different clotting factors help in the stoppage of the blood by causing mesh work
formation by fibrin deposition as well as causing aggregation of the platelets
forming blood clot each clotting factor is specifically involved in specific function
ultimately leading to stoppage of blood oozing out of wound

Eg. Prothrombin factor help in meshwork formation

Answer no 3:

Hemophillia

Von willibrand disease

DIC

Thrombocytopenia.
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