Table 1

Phytochemical screening of plant extract and synthesized nanoparticles.

S. no. Chemical constituents Screening

Plant extract AgNps

1 Alkaloids + –
2 Tannins + +
3 Carbohydrates + +
4 Ascorbic acid + +
5 Proteins + +

Free radical scavenging activity

1 ml of each of different concentrations (100–500 µg

in ethanol) of the synthesized silver nanoparticles and
standard (gallic acid) was added to 2.5 ml of 0.3 mM
DPPH in ethanol solution. The mixture was shaken
vigorously and allowed to stand at room temperature in
dark for 30 min. The absorbance was measured at 517
nm.

1. Results and discussion

When the leaf extract of A. sessilis was mixed with

AgNO3 solu- tion, the pale yellow colour of aqueous
extract changed to brownish colour immediately within
10 min, indicating the formation of sil- ver
nanoparticles (Fig. 1).
Phytochemical screening of A. sessilis leaf extract
shows the presence of alkaloids, tannins, carbohydrates,
proteins, ascorbic acid and synthesized AgNPs shows
the presence of tannins, car- bohydrates, proteins and
ascorbic acid (Table 1). These secondary
DOI: 10.3109/1040841X.2014.912200 Silver nanoparticles 2

290 K.L. Niraimathi et al. / Colloids and Surfaces B: Biointerfaces 102 (2013) 288–291

75.00

74.5

74.0

1864.11
2261.41 719.87493.99
3855.98 73.5 2381.972141.92 710.51
3729.72 2369.65 616.68 456.92
3710.08 2343.49
2324.82
3006.13 2300.57
2989.15
73.0
2892.78 1623.04
2855.69 1384.46

72.5
3423.57
%T 2926.69

72.0 1033.37

71.5

71.0

70.5

70.00
4000.0 3000 2000 1500 1000 413.0
cm-1

Fig. 3. FT-IR absorption spectra of bio-moieties with synthesized AgNPs.