Tabulation of Results

General test for Carbohydrates 

Molisch Test Anthrone Test Iodine Test

Color of Reagent Reddish-violet Yellow Purplish-black

Starch Amylose Positive (Purple-violet ring) Positive  Orange-brown

(Green)

Glycogen Positive (Purple-violet ring) Positive  Red-brown

(Green)

Cellulose Positive (Purple-violet ring) Positive  Purple

(Green)

Comparative Test for Reducing Sugars 

Fehling’s Test Benedict’s Test

Color of Reagent Blue Aqua Blue

Sucrose Negative (Blue) Negative (Blue)

Maltose Positive (Reddish-brown) Positive (Brick Red)

Lactose Positive (Reddish-brown) Positive (Orange)

Cellobiose Positive (Reddish-brown) Positive (Brick Red)

Glucose Positive (Reddish-brown) Positive (Orange)

Galactose Positive (Reddish-brown) Positive (Brick Red)

Mannose Positive (Reddish-brown) Positive (Brick Red)

Fructose Positive (Reddish-brown) Positive (Orange)

Ribose Positive (Reddish-brown) Positive (Yellow Green)

Xylose Positive (Reddish-brown) Positive (Brick Red)

Specific Tests for Carbohydrates

 Barfoed’s Test Mucic Acid Seliwannoff’s Test Bial’s Test
Test

Color of Light Blue Colorless Colorless Brown

Reagent

Sucrose Negative Negative Positive Negative

(Light Blue) (Colorless) (Red) (Brown)

Maltose Positive Negative Negative Negative

(Red – 20 mins) (Colorless) (Light Pink) (Brown)

Lactose Negative Positive Negative Negative

(Light Blue) (White) (Light Pink) (Brown)

Cellobiose Positive Negative Negative Negative

(Red – 20 mins) (Colorless) (Light Pink) (Brown)

Glucose Positive Negative Negative Negative

(Red – 5 mins) (Colorless) (Light Pink) (Brown)

Galactose Positive Positive Negative Negative

(Red – 5 mins) (White) (Light Pink) (Brown)

Mannose Positive Negative Negative Negative

(Red – 5 mins) (Colorless) (Light Pink) (Brown)

Fructose Positive Negative Positive Negative

(Red – 5 mins) (Colorless) (Red) (Brown)

Ribose Positive Negative Negative Positive

(Red – 5 mins) (Colorless) (Light Pink) (Blue)

Xylose Positive Negative Negative Positive

(Red – 5 mins) (Colorless) (Light Pink) (Blue)