Introduction of VET-SAA ‘Eiken’

for ACVP/ASVCP/ISACP Virtual Exhibition

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Index

1. About Feline Acute Phase Proteins…………………..……. p.3

2. SAA in other species…………………………………….……….. p.7
3. Product information - VET-SAA ‘Eiken’………………….. p.10
4. Clinical Performance Data – Feline SAA.................... p.13
5. Clinical Performance Data – Canine SAA.................. p.16

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Feline Acute Phase Proteins

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Acute Phase Proteins of Cat

• Serum amyloid A
Major APP (>10-fold increase). The increase occurs by 8h after
inflammation starts.

• α-1 acid glycoprotein

Major APP (>10-fold increase). The increase occurs by 24h after
inflammation starts.

• Haptoglobin
Moderate (<10-fold) increase within 24h

Tamamoto et al. J Vet Med Sci. 2008: 70:1247–1252.

Ceron et al. Vet Clin Pathol 34:85–99. 4
Changes of SAA levels found

• Infection (e.g. FIP)

• Inflammatory disease (e.g. Acute pancreatitis)

• Neoplasia (e.g. Malignant mesothelioma)

• Renal failure

Etc...

Tamamoto et al. J Vet Med Sci. 2008: 70:1247–1252 Cray et al. Comp Med. 2009;59:517–526.
Schmidt and Eckersall. Acta Vet. Beograd 2015, 65, 149-161. Sasaki et al. J Vet Med Sci. 2003;65:545–548.
Hansen et al. Vet Res Commun. 2006;30:863–872. 5
Measuring feline SAA – LTIA assay “VET-SAA”

VET-SAA has higher sensitivity and better dilution linearity

than the conventional method (LZ-SAA)

Measurement value of feline SAA Dilution Linearity of feline serum

Feline sample LZ-SAA VET-SAA
No. (μg/mL) (mg/L)
1 0.0 2.4
2 0.0 2.6
3 0.0 3.1
4 0.0 2.5
5 0.0 2.7
6 0.0 0.6
7 0.0 0.5
8 0.0 1.1
9 0.0 0.7
10 0.0 0.5

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SAA in multiple species

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Changes of SAA levels found

Canine Pyometra, Aspiration pneumonia, Infection (Babesia canis, Bordetella bronchiseptica, parvovirus)
*1,2,3,4

Acute pancreatitis, feline infectious peritonitis (FIP), hyperthyroidism, lymphoma, malignant

Feline mesothelioma, chronic renal failure, squamous cell carcinoma, polycystic kidney disease, immune-
*5
mediated hemolytic anemia

Bacterial infections (Rhodococcus equi pneumonia, drusen, infectious arthritis, sepsis), viral infections
(EHV-1, equine influenza virus), inflammation (abscess, pleurisy, peritonitis, colitis, enteritis, pneumonia,
Equine omphalophlebitis, tendovaginitis), neonatal septicemia, endotoxemia, neoplasms, ischemia (colic), trauma,
*6,7
control of postoperative complications, differentiation of infectious and non-infectious processes in the
poor foal
*1 Christensen et al. Can Vet J, 55. 2014; 161-168 *2 Jitpean et al. BMC Veterinary Research, 2014; 10, 273 *3 Milanovic et al. J Vet Intern Med. 2019; 33: 1686– 1694
*4 Ceron et al. Vet Clin Pathol 34:85–99. *5 Tamamoto et al. J Vet Med Sci. 2008: 70:1247–1252 *6 Cray et al. Comp Med. 2009;59:517–526.
*7 Jacobsen and Andersen, Equine vet. Educ. (2007) 19 (1) 38-46

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Changes of SAA levels found

Bovine Infection (Pasteurella haemolytica serotype 1, coronavirus, adenovirus), bovine respiratory diseases (e.g.
*1,2 Mannheima haemolytica), transport stress, mastitis

Elephant: herpesvirus infection*1, Elephant seal: parasitic infection*4, Falcon: aspergillosis*5,

Manatees: cold stress, trauma*3, Porcine: Infectious respiratory disease - mycoplasma, Actinobacillus
Others
pleuropneumoniae Serotype 5 *1,6, Rabbit: Encephalitozoon cuniculi infection*7, Rhesus monkey:
mycobacteriosis*8

*1 Cray, Vol 59, No 6, Comparative Medicine, December 2009 *2 Petersen et al. Vet Res. 2004;35:163–187 *3 Cray et al. J Zoo Wildlife Med. 2013;44(4):911–917
*4 Sheldon et al. J Zoo Wildl Med. 2017;48(3), 767-775 *5 Caliendo et al. J Avian Med Surg. 2013;27(2), 83-89 *6 Petersen et al. Vet Res. 2004;35:163–187
*7 Desoubeaux et al. PLoS One. 2017;19;12(7):e0177961 *8 Krogh et al. J Zoo Wildl Med. 2014;45(2):306-14

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Product information - VET-SAA ‘Eiken’

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VET-SAA: LTIA assay for various chemistry analyzers

Inflammation response can VET-SAA is reactive with

be clearly detected. multispecies SAA.
Horse SAA data adopted from: Jacobsen, S., Vinther, A.M., Kjelgaard-Hansen, M. et
al. BMC Vet Res 15, 462 (2019).

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Have a trial - We are waiting on live chat!

VET-SAA ‘Eiken’
Serum amyloid A is an acute phase protein that
increases dramatically when inflammation occurs.
Designed for various chemistry analyzers

We offer collaboration kits for those who participate in

ACVP/ASVCP/ISACP 2020!
- Collaboration trial kit for lab testing
- Collaborative research
- Business collaboration
- Sales Distributor Recruitment
For more product information: https://www.eiken.co.jp/en/products/vet_saa/
Or please ask us at our virtual booth or by the contact form:
https://www.eiken.co.jp/en/inquiry.html
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VET-SAA Product information

VET-SAA
Product name VET-SAA ‛Eiken′ Reagent VET-SAA-QC-Low VET-SAA-QC-High
Calibrator Set

Product code V-SZ51 V-SZ90 V-SZ91 V-SZ92

Calibrator 5x1 mL Control 5x1 mL

R1: 2x20 mL Control 5x1 mL
Contents Buffer 1x12mL Buffer 1x12mL
R2: 2x20 mL Buffer 1x12mL
*freeze-dried product *freeze-dried product

Now preparing parameters of various chemistry analyzers as follows: Cobas(Roche), Pentra, AU（Beckman), Biolis, Daytona(Randox),
Abbott, Canon-TOSHIBA, JEOL, etc. Please contact us. https://www.eiken.co.jp/en/inquiry.html
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Feline SAA Clinical Performance Data

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Feline SAA Clinical Performance Data

■ Serum amyloid A (SAA) concentrations in serum from healthy and inflammatory-diseased cats
determined by VET-SAA.

Statistical significance:
P = 0.00013 P < 0.05 Significant
(Mann–Whitney U test)

In house DATA; Not published

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Feline SAA Clinical Performance Data

■ Feline serum amyloid A (SAA) concentrations determined in the clinically diagnosed groups of
organ systems by VET-SAA.

Statistical significance: Mann–Whitney U test,

vs. healthy cats, *Significant P < 0.05
P Value
Respiratory and circulatory diseases 0.218

Digestive diseases 0.223

Urinary and reproductive system 0.137

diseases
Blood tumor 0.000*

Infectious diseases 0.001*

In house DATA; Not published

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Canine SAA Clinical Performance Data

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Canine SAA Clinical Performance Data

■ Serum amyloid A (SAA) concentrations in serum from healthy and inflammatory-diseased dogs
determined by VET-SAA.

Statistical significance:
P = 0.00631 P < 0.05 Significant
(Mann–Whitney U test)

In house DATA; Not published

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 Canine SAA Clinical Performance Data

■ Canine serum amyloid A (SAA) concentrations determined in the clinically diagnosed groups of organ systems by VET-SAA.

P Value
Respiratory and circulatory diseases 0.003*
Digestive diseases 0.008* Statistical significance: Mann–Whitney U test,
Urinary and reproductive system diseases 0.295 vs. healthy cats, *Significant P < 0.05
Blood tumor 0.693
Musculoskeletal system 0.006* Respiratory and circulatory diseases: Lower
Skin diseases 0.995 value than healthy dogs
In house DATA; Not published
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 Canine SAA Clinical Performance Data

■ Canine serum amyloid A (SAA) concentrations determined in the clinically diagnosed groups of diseases by VET-SAA.

P Value
Neoplasia 0.012*
Digestive diseases 0.002*
Inflammatory disease 0.295
Infectious diseases 0.519
Blood 0.581 Statistical significance: Mann–Whitney U test,
Endocrine 0.275 vs. healthy cats, *Significant P < 0.05
In house DATA; Not published
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