LAB REPORT BIOCHEMISTRY

(BIO 462)

EXPERIMENT 7: QUALITATIVE ANALYSIS OF

LIPIDS

NAME IMAN FARHA HANISAH BINTI DANIEL HASNI

STUDENT NUMBER 2019702307
GROUP AS2464B
LECTURER’S NAME DR NUR NADIAH MD YUSOF
DATE OF EXPERIMENT 11TH MAY 2020
 BIO462 Biochemistry Practical
Qualitative analysis of lipids

1. Suggest and draw a molecule that contains three fatty acid chains bonded to a
glycerol molecule? (2 marks)
Triglycerides

2. Does the chemical structure below is saturated or unsaturated fats? Give a

reason for your answer. (2 marks)

Saturated fat because there is no double bond

3. Classify the food item below either saturated or unsaturated fat. (5 marks)

No. Food item Saturated / unsaturated fat

1. Meat Saturated
2. Tuna Unaturated
3. Almond seeds Unsaturated
4. Olives oil Unsaturated
5. Cheese Saturated
Solubility Test

Table 1: Solubility in water and chloroform of two food samples

No Food Water Chloroform

1 Sample A immiscible miscible
2 Sample B miscible miscible

4. Based on Table 1, describe the solubility of Sample A in water and in

chloroform. (2 marks)
Sample A is does not dissolve in water but completely dissolve in chloroform.
5. Make a conclusion on Sample A? (1 mark)
Sample A contain fats and oils
Translucent Spot Test
6. Describe how the lipid in the food products such as Desi ghee, vegetable ghee
or refined oil can be detected just using a filter paper? (2 marks)
The filter paper was pressed on food sample and absorbed the lipid in food, creating a
translucent spot
Acrolein Test
7. Describe the reactions involve during the Acrolein test. (4 marks)
When oil or fat is treated strongly in presence of dehydrating agent like potassium
bisulphate, the glycerol portion of the molecule is dehydrated to form an unsaturated
aldehyde, acrolein which has a pungent irritating odour.
Huble’s Test
8. What is the main purpose of Huble’s test? (1 marks)
To determine the degree of unsaturatedness in oil
9. Explain the function of Huble’s reagent in the experiment. (3 marks)
Huble's reagent reacts with alcoholic solution of iodine that contains some mercuric chloride.
During the reaction, the violet colour of iodine fades away if the oil or fat is unsaturated.
Sudan IV Test
10. In the Sudan IV test, how is the distribution of the dye with respect to the
separated water and oil? (2 marks)
Water solution has a lighter in colour than oil solution that also have a red layer present in it.
11. What observation indicates a positive test for lipid in Sudan IV Test? (2 marks)

A dark red layer is formed, indicating the Sudan IV has stained the oil present in the test