‘Max.Marks: 25 Attempt all questions Q.1.a. The temperature at which proteins unfold increases by as much as 40°C when ‘water is removed either by drying or transferring the protein to a non-aqueous solvent. Do you think this statement is correct? Justify your answer. 3 ». Do you think log P (partition coefficient between aqueous/organic phase) values are going to equally affect enzyme stability of different enzymes? Explain. 2 ©. Show schematically how pleated Sheet can be formed in a mixed beta-sheet. Why do you think mixed sheets are not that common? 2 Q2 a. What is the difference between a-helix and 3,9 helix, show schematically? How could the presence of these structures recognized in the polypeptide chain? Which structure would be more stable and why? 3 b, What is the basic functional unit of a protein? Explain the hierarchy of protein organization defining the various levels and the relationship among these? 2 Q.3 a. Examine the figure on the next page. Represent it thru a topology diagram, Where might be the active site located (show on the topology diagram). 2 ». What kind of amino acid stretch are you going to see of the motifs that are a part of beta barrel vis a vis beta-alpha-beta barrel . Give example of a protein from each category with its function. 3 . What are the likely problems that may arise during “Inverse PCR? methods? Show the products after second and third round of replication. Name an experimental condition. that can be adopted to overcome this? 3 4. PCR methods often lead to addition of extra A residues at the 3’end of DNA which poses a problem during ligation of these fragment. What method could you use to solve this problem? 2 Qa Whatis the secondary structure of antibodies like? Do the cells enjoy any advantage by having antibodies in this secondary conformation? 3BELT14 Protein Science and Engineering Re-Minor-1, 1" semester,2014-15 Attempt all questions ‘Time: 1 hour Max. Marks; 25 ~ Q.1 a) Write down the structure of the following tetra-peptide, along with their single letter code words: Hiselle-Glu-Pro-Tep 5 b) What is the difference between a motif and a domain? Give an example of each along with their function. 3 ) What is the structural hierarchy in SCOP and explain the basis of classification into various sections. 3 Q2 a) Examine the following stretch of amino acids known to be in an alpha helix. Place these on the helical wheel and predict the location of the helix in the protei PEEAQRKLRILQRELQNV 3 Q.3. Describe the models put down for helix-helix packing . Give suitable examples to support your models, Would you expect this packing to be affected by amino acid sequence? Support your answer with concrete examples. 5 Q4 Explain why: 4) binding crevices for antigens are localized in the loop regions of antibodies. b) Analpha helix has a distinct polarity ©) Amino acids are optical rotatory.