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Lab 5 Schematic Diagram
Lab 5 Schematic Diagram
Ninhydrin Rxn
Prepare on separate test tubes: 0.5mL of saturated solutions of casein, albumin, glycine, and
H2O
Add 2-3 drops 0.1% Ninhydrin Solution to each sample
Cover each sample with a marble
Water bath to boil for 1-2mins
Cool
Observe
B. Biuret Test
Prepare on separate test tubes: 0.5mL of saturated solutions of casein, albumin, glycine, and
H2O
Mix 0.5mL 10% NaOH to each sample
Add 1-2 drops 0.5% CuSO4 in each sample
Observe
C. Xanthoproteic Test
Prepare on separate test tubes: 0.5mL of saturated solutions of casein, albumin, tryptophan,
tyrosine, phenylalanine, histidine, and H2O.
Add 0.5mL conc. HNO3 to each sample
Observe for heavy white precipitate is formed
Heat and Observe for formation of yellow color
Record color of solution
Cool
Make alkaline with 10% NaOH.
Observe
D. Millon-Nasse Test
Prepare on separate test tubes: 0.5mL of saturated solutions of casein, albumin, tyrosine, and
H2O
Add 3 drops Millon-Nasse reagent to each sample
Water bath to boiling for 10 mins.
Cool in ice bath
Add 0.5mL 0.1% NaNO2
Warm gently
Observe
E. Hopkins-Cole Reaction
Prepare on separate test tubes: 0.5mL of saturated solutions of casein, albumin, tryptophan,
and H2O
Mix 0.5mL Hopkins-Cole reagent to each sample (observe presence of violet ring)
Layer each sample with 1mL conc. H2SO4
Observe
Prepare on separate test tubes: 0.5mL of saturated solutions of casein, albumin, tryptophan,
and H2O
Add 2 drops of bromine water to each sample
Shake each sample with 0.5mL n-amyl alcohol
Observe
G. Pauly Reaction
Prepare on separate test tubes: 0.5mL of saturated solutions of casein, albumin, histidine,
tyrosine, and H2O
Mix 0.5mL cold sulfanilic acid with 0.5mL cold 0.5% NaNO2 to each sample
Cool in ice with constant shaking for 3 mins.
Add the specified amino acid and protein samples in separate test tubes with 10% Na2CO3
Observe
Prepare on separate test tubes: 0.5mL of saturated solutions of casein, albumin, cysteine, pcs of
hair, and H2O
Add 0.5mL 20%NaOH and 2 drops 10% Pb(OAc)2 in each sample
Cover each sample with a marble
Water bath to boiling for a few mins.
Observe
I. Sakaguchi Reaction
Prepare on separate test tubes: 0.5mL of saturated solutions of casein, albumin, arginine, and
H2O
Mix 1mL of 20% NaOH in separate test tubes in each sample
Add 0.5mL Molisch reagent in each sample
Mix thoroughly
Add 0.5mL bromine water in each sample
Observe