OCT.

30, 2020 ACTIVITY: QUALITY CONTROL

24 - LEONIDA, Julyan
25 - LINSANGAN, Alexandra Marie
26 - LIU, Danielle D.
27 - MACAPUNDAG, Aisha A.
28 - MACATANGAY, Richard Lee Roy P.
29 - MANALO, Rachel Ann S.

GROUP 2B

4CMT - Tondo Medical Center

 Blood Storage
Issue Transport
Component Shelf Life Temperature

Whole Blood CPD: 21 days 1°C - 6°C ● Volume expansion, Increased O2 ● Must be kept in a cold box or insulated carrier to
CPDA: 35 days ● Recipient is ABO and RhD compatible keep the temperature within the 1°C - 6°C range
CPD2D: 21 days ● There must be no additional medication to ● Transportation should be accomplished within
ACD: 21 days the unit of blood 24 hours

RBCs 24 hours 1°C - 6°C ● IgA-Negative Person, PNH ● Must be kept in a cold box or insulated carrier to
keep the temperature within the 1°C - 6°C range
● Note that they should not be frozen

Frozen RBCs 10 years ≤ -65°C ● Rare Phenotypes ● Maintain frozen state

RCCs 24 hrs 1°C - 6°C ● Prolonged red cell storage for rare blood ● Transport at 1°C - 10°C; On ice or with frozen
Deglycerolized units and autologous storage chemical coolant in ARC boxes or cooler

Washed RBCs 24 hrs 1°C - 6°C ● Reduce plasma proteins to avoid allergic and ● Transport at 1°C - 10°C; On ice or with frozen
anaphylactic reactions (IgA deficiency) chemical coolant in ARC boxes or cooler

Irradiated RBCs 28 days from 1°C - 6°C ● Prevent GVHD in immunocompromised ● Transport at 1°C - 10° C; On ice or with frozen
irradiation patients, HLA -matched platelets,or chemical coolant in ARC boxes or cooler
transfusions from blood relative

RBC Leukocytes 42 days 1°C to -3°C ● Transfused through a 170–200 µm filter ● Blood component, transit containers, packing
Reduced for up to 4 ● Transfusion should be within 4 hours of issue materials, and procedures should be validated
hours out of a controlled temperature environment ● Surface temperature should be maintained
● RBCs compete for nutrients with WBCs between 2°C and 10°C
unlike the leukodepletion method that ● Minimize dead air space in package
provides RBCs with an appropriate
concentration to last longer.
 PF24 1 year ≤ -18°C ● 20% to 30% loss of FVIII activity ● Maintain frozen state
● Patients with liver disease
● DIC

FFP 1 year ≤ -18°C ● Coagulation deficiency ● Maintain the blood component at ≤ -18°C or ≤
7 years ≤ -65°C ● Patients with liver disease -65°C
● DIC

FFP Thawed 24 hrs 1°C - 6°C ● If the thawed FFP is not used within 24 hrs, it ● N/A; Once thawed the FFP can not be refrozen
should be discarded. Once thawed, the & has to be discarded if it has passed its
activity of clotting factors, particularly factor V expiration.
and VIII will decline gradually.

Plasma Thawed 5 days after 1°C to 6°C ● If not transfused within 24 hours, FFP should ● N/A; Once thawed the FFP can not be refrozen
thawing be relabeled as “thawed plasma” and should & has to be discarded if it has passed its
not be used for replacement of labile expiration.
coagulation factor VIII.

Cryoprecipitated 1 year ≤ -18°C ● Factor XIII deficiency ● Transport at -18°C or lower; In ARC boxes or
AHF ● Fibrinogen deficiency cooler with dry ice.

Pooled 4 hrs 20°C - 24°C ● Indicated for fibrinogen deficiency, is a ● Pooled CRYO must be administered within 4
Cryoprecipitate second line therapy for von willebrand hours of first entry and should be stored at room
disease, and hemophilia A temperature until transfusion.

Platelets 5 days with 20°C - 24°C ● Your platelets will clot (clump together) to ● Platelets should be transported in a blood
gentle agitation plug the hole in the blood vessel and stop the transport box that keeps the temperature in the
bleeding. You can have different problems correct range of about 20​°C ​– 24​°C
with your platelets: If your blood has a low ● Insulated container or have a special gel pouch
number of platelets, it is called to have a consistent temperature
 thrombocytopenia. ● Ice packaging would not be necessary

Pooled Platelets 4 hrs 20°C - 24°C ● Bacterial contamination is a concern and is ● Units are usually issued within 2 hours of order
higher in pooled platelets than apheresis receipt (routine) or to arrive by the specified
units date/time
● Storage Pool Disease (SPD) can occur, ● Pooled platelets ordered “Emergency” leave
which is when the platelet granules are PSBC within 60 minutes of order receipt.
affected. ● Requires additional time for transport

Apheresis Platelets 5 days 20°C - 24°C ● Need of full control in overall process to ● Containers chosen should be at room
Leukocytes maintain adequate amount of therapeutic temperature before usage
Reduced
blood cells intended for the transfusion ● Maintain the temperature close to the
6 recommended storage temperature
● Need to have less than 5 × 10 residual
● Once arrived, it should be transferred at 22 ±2°C
leukocytes in 95% of units
11 with continuous gentle agitation.
● Need ≥ 3 × 10 platelets in 75% of units
● Remove plastic overwraps before storage
tested
● pH should be ≥ 6.2

Apheresis Administered 20°C - 24°C ● Neutropenia (generally <0.5 x109/L or ● Transport at 20°C - 24° C
Granulocytes within 24 hours without 500uL)
of collection agitation ● Unresponsive to antibiotics
until ● Documented infections, especially Gram (-)
transfused bacteria or fungi
 REFERENCES
AABB Temperature Standards 2018. (2018). Retrieved November 03, 2020, from https://temptimecorp.
com/support/safe-t-vue-support/aabb-temperature-standards-2016/
Agus, N., Yilmaz, N., Colak, A., & Liv, F. (2012). Levels of factor VIII and factor IX in fresh-frozen plasma produced from
whole blood stored at 4 °C overnight in Turkey. Retrieved November 03, 2020, from
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3320779/
Harmening, D. (2019). Modern blood banking & transfusion practices. Philadelphia, PA: F.A. Davis Company.
Howard, P., & Blaney, K. (2017). Basic & applied concepts of blood banking and transfusion practices (p. 131). St. Louis,
MO: Elsevier.
Joint UKBTS Joint Professional Advisory Committee. (2016). JPAC - Transfusion Guidelines. Retrieved November 03,
2020, from https://www.transfusionguidelines.org/red-book/chapter-7-specifications-for-blood-components/7-5-red-
cells-leucocyte-depleted
University of Toledo Medical Center. (2019). Blood and Component Storage, Expiration and Transportation. Retrieved
October 30, 2020, from https://www.utoledo.edu/policies/utmc/bloodbank/pdfs/3364-108-202.pdf