I.

HASIL DAN PERHITUNGAN

A. Absorbansi dan Kurva Kalibrasi

1. Kafein
A=A 1% 1cm x b x C
A
A 1 % 1 cm= x 10000 ppm
C ( ppm )

Absorbansi A1%1cm

λmax as. λmax as.

C λmax Kafein λmax Kafein
Benzoat Benzoat
(273nm) (273nm)
(230nm) (230nm)

4 0.2064 0,1016 516 254

6 0,3097 0,1524 516,166 254
8 0,4129 0,2032 516,125 254
10 0,5161 0,2540 516,1 254
12 0,6193 0,3047 516,083 253,917
a=0 a= 0,00006
Rata-rata Rata-rata
b = 0,05161 b = 0,02539
516,095 253,983
r = 0,999 r = 0,999
y = 0,00006 +
y = 0,05161x
0,2539x
2. Asam Benzoat

A=A 1% 1cm x b x C
A
A 1 % 1 cm= x 10000 ppm
C ( ppm )

Absorbansi A1%1cm

λmax as. λmax as.

C λmax Kafein λmax Kafein
Benzoat Benzoat
(273nm) (273nm)
(230nm) (230nm)

4 0.0344 0,3736 86 934

6 0,0516 0,5604 86 934
8 0,0688 0,7473 86 934,125
10 0,0860 0,9341 86 934,1
12 0,1032 1,1209 86 934,083
a=0 a=-0,00006
Rata-rata Rata-rata
b = 0,0086 b = 0,0934
86 934,0616
r=1 r = 0,999
y = 0,0934x
y = 0,0086x
-0,00006
B. Penetapan Kadar Kafein dan Asam Benzoat
1. Data Absorbansi Sampel dan Perhitungan Konsentrasi Kafein dan Asam
Benzoat

Absorbansi
Replikasi
λ 273nm λ 230nm

S1 0,7824 1,4356
S2 0,7731 1,4831
S3 0,7795 1,3958

Sampel 1 = 0,7824 = 516,095 . C.kafein + 86 . C.as. benzoat x 253,983

1,4356 = 253,983. C.kafein + 934,062 . C.as. benzoat x 516,095

198,716 = 131079,356 . C.kafein + 21842,538 . C.as. benzoat

740,906 = 131079,356 . C.kafein + 482064,728 . C,as. bnzoat

- 542,19 = - 460222,19 .C.as. benzoat

C.as. benzoat = 0,001178 % = 11,781 ppm

0,7824 = 516,095 . C.kafein + 86 . C.as. benzoat

0,7824 = 516,095 . C.kafein + 86 . 0,001178

0,7824 = 516,095 . C.kafein + 0,1013

0,7824−0,1013
C kafein=
516,095

= 0,0013197% = 13,197 ppm

Sampel 2 = 0,7731 = 516,095 . C.kafein + 86 . C.as. benzoat x 253,983

1,4831 = 253,983. C.kafein + 934,062 . C.as. benzoat x 516,095

196,354= 131079,356 . C.kafein + 21842,538 . C.as. benzoat

765,420 = 131079,356 . C.kafein + 482064,728 . C,as. bnzoat

- 569,066 = - 460222,19 .C.as. benzoat

C.as. benzoat = 0,0012365 % = 12,365 ppm

0,7731 = 516,095 . C.kafein + 86 . C.as. benzoat

0,7731 = 516,095 . C.kafein + 86 . 0,0012365

0,7731 = 516,095 . C.kafein + 0,1063

0,7 731−0,10 6 3
C kafein=
516,095

= 0,001292% = 12,920 ppm

Sampel 3 = 0,7795 = 516,095 . C.kafein + 86 . C.as. benzoat x 253,983

1,3958 = 253,983. C.kafein + 934,062 . C.as. benzoat x 516,095

197,980 = 131079,356 . C.kafein + 21842,538 . C.as. benzoat

720,365 = 131079,356 . C.kafein + 482064,728 . C,as. bnzoat

- 522,385 = - 460222,19 .C.as. benzoat

C.as. benzoat = 0,001135 % = 11,351 ppm

0,7795 = 516,095 . C.kafein + 86 . C.as. benzoat

0,7795 = 516,095 . C.kafein + 86 . 0,001135

0,7795 = 516,095 . C.kafein + 0,0976

0,7 795−0 ,0976

C kafein=
516,095

= 0,0013213% = 13,213 ppm

2. Kandungan Kafein dan Asam Benzoat dalam Minuman Kratingdaeng
Dalam praktikum ini diambil 1 ml kratingdaeng kemudian dianalisa dengan
spektrofotometri. Diperoleh hasil seperti berikut.
a) Konsentrasi Kafein
Sampel 1 : 0,0013197%
Sampel 2 : 0,001292%
Sampel 3 : 0,0013213%
Rata-rata : 0,001311%
Jadi, kandungan kafein dalam 1 ml minuman kratingdaeng sebesar

0,001311 g
x 1 ml=0,00001311 g=0,01311 mg
100 ml
b) Konsentrasi Asam Benzoat
Sampel 1 : 0,001178 %
Sampel 2 : 0,0012365 %
Sampel 3 : 0,001135 %
Rata-rata : 0,001183%
Jadi, kandungan asam benzoat dalam 1 ml minuman kratingdaeng

0,00 1183 g
sebesar x 1 ml=0,00001 183 g=0,01 183mg
100 ml