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Sonal Bhuta Plan An Investigation Using Dialysis Tubing To Determine The Effect of Temperature On The Activity of The Enzyme Amylase
Sonal Bhuta Plan An Investigation Using Dialysis Tubing To Determine The Effect of Temperature On The Activity of The Enzyme Amylase
10:01 AM
Some plants have leaves that are not green. For example the plant may have red, purple or yellow
leaves. A student predicted that leaves of different colours would have different rates of
photosynthesis. Plan an investigation to find out if the student’s prediction is correct.
Sonal Bhuta
10:02 AM
Plan an investigation using dialysis tubing to determine
the effect of temperature on the activity of the enzyme
amylase.
ANS:
(at least) two different temperatures used ; method of maintaining constant temperatures ; max two
from given method ;; add enzyme (solution) to starch (suspension) in dialysis tubing rinse tubing and
place in test-tubes with distilled water testing with iodine / Benedict’s solution same volume of enzyme
/ amylase same volume of substrate / starch same volume of (distilled) water in test-tubes new
method: same, enzyme / amylase solution concentration ; same. substrate / starch suspension
concentsame, enzyme / amylase solution concentration ; same. substrate / starch suspension
concentration ; performing each temperature at different times ; testing for results at set time
intervals ; use of spotting tile ; two more repeats (for each temperature) ; use of gloves / goggles /
tongs / test-tube holders
ANS: Q1
1 using at least 2 different colours of leaf ; 2 identifying the dependent variable ; e.g. oxygen / gas
production / indicator colour change 3 measuring (dependent variable) after a set time ; 4 method of
collecting gas in a set time ; e.g. use of a measuring cylinder / gas syringe / counting bubbles / how
long it takes leaf discs to surface 5 method to control temperature ; 6, 7, 8 three from: ;;; • same
surface area / size / age, of leaf / plant • same temperature • same light, intensity / amo
6, 7, 8 three from: ;;; • same surface area / size / age, of leaf / plant • same temperature • same light,
intensity / amount • same carbon dioxide, concentration / amount • same volume / amount of water •
same volume / concentration / amount, of indicator 9 2 or more repeats / three or more trials of each
leaf colour ;
Sonal Bhuta
10:18 AM HODDER
exp 6