EXPERIMENT NO.

5
LIPID CHEMISTRY
Rubrics
NAME: Kimberly Kate C. Bullos 1. Submitted on time 3
2. Neat & well-organized report 3
GRP. NO.: CARBON 3. Data collected & recorded accurately
GROUP MEMBERS: Buenviaje, Patricia reflects result of expt. 7
4. Connects scientific principles in the 7
Kimmayong , Danilyn discussion of results
TOTAL POINTS 20
Patricio, Monique Nicole
SECTION: 2PSY01 DATE: 11/21/20
LAB INSTRUCTOR: Ma’am Cynthia Omampo

A. DATA & RESULTS

A. Solubility: Write: I = immiscible SI = slightly immiscible IM = immiscible

CHCl2 C6H6 C2H5OH CCl4 H2O NaOH

Olive oil

Coconut oil

B. Spotting Effect : Observations

Oil in filter paper + ethanol ______________________________

Oil in filter paper + ether ______________________________

C. Test for Unsaturation

Sample Volume in mL of I2 used

  1. olive oil __________

2. coconut oil __________

3. stearic acid __________

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 D. Saponification

Observations:_____________________________________________________________________

________________________________________________________________________________

  Equation Involved:

E. Acrolein Test

Observations:____________________________________________________________________

_______________________________________________________________________________

Equation Involved:

F. Test for Cholesterol

1. Leibermann-Burchard

Observations:____________________________________________________________________

_______________________________________________________________________________

2. Salkowski Test

Observations__________________________________________________________________

_____________________________________________________________________________

G. Emulsification of Fats

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 1. 1 mL water + drop coconut oil

2. 1 mL water + 1 drop coconut oil + 1 drop Na 2CO3 solution

3. 1 mL water + 1 drop coconut oil + a pinch of soap

Observations____________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

  II. DISCUSSION OF RESULTS

A. Solubility
________________________________________________________________________________

________________________________________________________________________________

_________________________________________________________________________________

B. Spotting Effect
_________________________________________________________________________________

__________________________________________________________________________________

C. Test for Unsaturation

____________________________________________________________________________________

____________________________________________________________________________________

D. Saponification
_____________________________________________________________________________________

__________________________________________________________________________________

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 E. Acrolein Test
__________________________________________________________________________________

__________________________________________________________________________________

F. Test for Cholesterol

1. Leibermann’s Test
_________________________________________________________________________________

________________________________________________________________________________

2. Salkowski Test
_________________________________________________________________________________

_________________________________________________________________________________

G. Emulsification of Fats
_________________________________________________________________________________

__________________________________________________________________________________

III. ANSWER THE FOLLOWING: (to be accomplished individually)

1. Differentiate fats from oils on the basis of their structure?

2. What is the importance of Acrolein Test? What component in fats and oil will respond to the
test?

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3. What is an emulsifying agent? Give examples and its application in the body, at home and in the
industry.

4. Explain the cleansing property of soap?

One of DOH’s protocol to prevent COVID transmission is frequent hand washing, illustrate the

mechanism on how soap attacks Corona virus.

5. Give the clinical significance of the following:

a. Saturated and unsaturated fatty acids   

b. Cholesterol

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