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12 STEM Group 2
12 STEM Group 2
12 STEM Group 2
___________________________
A Research Paper
Presented to
the Faculty of Senior High School Department
Southwestern University PHINMA
Cebu, City
___________________________
In Partial Fulfillment
of the Requirements for the Subject
Practical Research 2 (APP 006)
___________________________
By:
EBORDA, ROMEL
GUMAPAC, DWYNWEN
iv
October 2019
APPROVAL SHEET
Approved in partial fulfillment of the requirements for the subject Practical Research 2
(APP 006) by the Proposal Hearing Committee
ACCEPTED in partial fulfillment of the requirements for the subject Practical Research 2
(APP 006).
D. The Problem
b. Research Hypothesis...................................................................................... 9
E. Research Methodology
a. Research Design............................................................................................... 11
b. Research Environment...................................................................................... 11
c. Research Subjects............................................................................................ 12
f. Statistical Treatment
v
CHAPTER II
Summary
Findings
Conclusion
Recommendations
BIBLIOGRAPHY ..................................................................................................................... 15
RESEARCH DOCUMENTATION
APPENDICES ......................................................................................................................... 18
CURRICULUM VITAE
v
CHAPTER I
This section presents the important facts that support the validity of the data presented
The researchers are concerned of the harmful effects that pesticides cause as they are
used widely in agriculture, homes, schools, and other places all over the world. As well as the
fact that mosquito-borne diseases are getting worse every year, with dengue being the most
In an article written by the Vector Disease Control International (VDCI) team (2019),
West Nile virus, another mosquito-borne disease, infected 49 out of 50 states in the USA and
the District of Columbia. A total of 2, 647 cases were reported and 167 confirmed deaths in the
year 2018.
(2018), dengue fever and dengue hemorrhagic fever are transmitted by the female Aedes
mosquito. They stated that a total of 69,088 dengue cases were reported nationwide from
January 1- July 28, 2018. In another report from reliefweb.int (2019), they mentioned that
WHO confirmed 271, 480 dengue cases, along with 720 deaths, were reported from January 1
– August 31, 2019. The results were 113% higher than in 2018. They also mentioned that the
The researchers came up with an idea to produce a safe and non-toxic pesticide that
can effectively mitigate mosquitoes and is free from chemicals and purely made with organic
This section presents the works of different authors that are relevant to this study. It
contains quotations from various reference materials which were used as a basis for the
manzanilla, but taller. In the Philippines, the flower heads are usually white or various colored
(Philippine Medicinal Plants, 2018). It, especially the oil and extract, is usually used in
medicinal practices and making herbal medicine for its antibacterial and antibiotic properties.
(WebMD, 2018). These flowers are usually observed to study plant regeneration because of its
ability to grow branches when a part is cut off (Chen et al, 2019).
neurotoxin that can kill insects but does not harm mammals or birds. According to MGK's
article 'Pyrethrins & Pyrethroids', pyrethrins are found at particularly high concentration
within flower structures known as achenes which are located in the flower head of the
Insecticidal properties of the flower genus Chrysanthemum are called Pyrethrum and
Pyrethrins. Chrysanthemum flowers are also called “insect flowers”. Pyrethrum is the approved
As stated in Amal et al's study (2008), the extract from Chrysanthemum coronarium L.
and its fractions have shown insecticidal effect on cotton leaf worms. The flower extract
3
seemed to be a potential plant for application in integrated pest management because of its
In a similar study, researchers from the University of Baghdad in Iraq (Shawkat et al,
2011), extracted the crude active compound of pyrethrin to produce an insecticide made of
natural products as a substitute to synthetic ones. Different concentrations (20%, 30% and
40%) of the extract were used against flour beetles (Tribolium castanum).
contains aromatic oil. Its name is derived from the lemon-like odor of the essential oil present
in the stalks. Lemongrass oil is commonly used in culinary flavoring, medical practices and
most countries. In Brazilian folk medicine, it is used to treat nervous and gastrointestinal
boiling water on fresh or dried lemongrass leaves (this is infusion is called "abafado" in
Lemongrass oil is an effective insecticide against ticks, termites and dust mites and
various studies have shown lemongrass oil to be anywhere from 50 to 95% effective at
repelling insects for at least two to three hours according to study results as summarized in the
Lemongrass is also an effective insect repellent; it can repel various insects and
mosquitoes. Lemongrass is a natural repellent because of its high geraniol and citral content.
You can mix lemongrass oil with lemon eucalyptus oil for mosquito protection of about five to
six hours per application. You can also mix lemongrass oil with rosemary oil for a more
fragrant formula. This can ward off a greater number of insect species (Wells, 2017).
In addition to being used as a medicine, lemongrass can also be used in the food
industry, cosmetic industry and pharmaceutical industry as well. Ethanol extract from
lemongrass have shown anti-bacterial activity against Staphylococcus aureus and Salmonella
typhi. These ethanol extract contain protein, fats and carbohydrates (Nimrat et al, 2018).
Pests pose the serious threat to every living thing. There are many different kinds of
pests including fungus, mosquitoes, flies, rats, fleas, termites, feral dogs and any living
organism that has a harmful effect on humans, their surroundings, stock and food
supplies(Sharif, 2019).
There are approximately 3,000 different species of mosquitoes. Only the female
mosquitoes bite and suck blood because they need protein to help their eggs develop properly.
Mosquito-borne diseases are caused by the bacteria, viruses or parasites that were
transmitted by the mosquito when it is feeding. These are the most prominent mosquito-borne
diseases to date; Malaria, West Nile Virus, Yellow Fever, Dengue, Chikungunya, and Zika
Of all the household pests in the world, mosquitoes are the most dangerous of them all.
situation of tropical countries like the Philippines. Dengue, the world’s fastest-spreading
Salazar, 2018).
5
Insecticides are types of pesticide that have chemical and biological origins. They are
used to control vectors, such as mosquitoes and ticks, which spread human and animal
According to 'Homeowner's Guide To: Pesticide Use Around the Home and Garden'
(Bauder et al, 2018), pests in the home and garden can cause damage to homes and
landscapes, health problems, and decrease the production of fruit and vegetables. The use of
pesticides can cause damage to our health and environment. Products that mitigate insects
(insecticides), weeds (herbicides), and fungi (fungicides) are pesticides. The components that
make these chemicals toxic to pests also can be harmful to people and animals. In some
Commercially available chemical pesticides are currently the most and only effective
means of pest control program. However, the frequent use of these chemical substances has
not only caused adverse effects on mammals’ health, but has also caused harmful impact on
the other beneficiary members of the ecosystem and the environment in which they are
DEET, a chemical that is used in insect repelling products (e.g. sprays, lotions, etc.),
prevents bites from mosquitoes, biting flies, fleas and other small flying insects. It is a colorless
liquid with a faint odor and it does not dissolve easily in water. DEET was developed by the
U.S. Army in 1946 to protect soldiers from insect-infested areas. Since 1957, insect repellents
containing DEET have been used by the general public in the United States. It was estimated
that about 30% of the U.S. population uses one or more products that contain DEET every
6
According to Bloomquist (2015), pyrethroid insecticides are widely used compounds,
typically esters of chrysanthemic acid having a high degree of lipophilicity (fat solubility). The
original compounds in this series were the natural pyrethrins (e.g., pyrethrin I), which are
Pyrethrins. They provide long-lasting control and improved mammalian safety relative to other
products in use at the time they were developed. These compounds are commonly effective
against caterpillars, beetles, certain aphids and mites in crops, and for mosquito, termite and
One alternative for insect pests control and to mitigate their attack is by using
Chemical constituents present in plants have these activities and they show promise in
fighting pests in agriculture. An approach to these new insecticides that fulfill the requirements
of safety, efficacy and selectivity, can be by studying the defense mechanisms of plants.
Recent studies have shown the potential of insecticide substances and extracts isolated from
plants against various insect pests that cause serious damage to crops or stored grain. These
active ingredients are distributed by different plant organs and involved in secondary
metabolism. They are divided into three major groups: terpenes, phenols and nitrogenous
Pesticides, especially insecticides, are used to control various pests and disease
carriers. Mosquitoes, ticks, rats and mice are some of the common targets. Vector-Borne
7
Diseases, Asthma and Allergies, Microbial Contamination are some examples of health
Insecticides are highly effective emergency tools for Integrated Pest Management when
the insect pest population exceed economic threshold. Despite its advantages, insecticide use
has led to many environmental problems. Insecticide resistance from pests is caused by
According to Merchant (2016), most consumers relate pesticides with pollution, health
risk, and toxic chemicals. About 75% of the consumers are wary of using pesticides. Extracts
(aqueous or oil) of some plant materials are toxic to some species of insect pests of crops
and others are less toxic. These extracts have lethal effects on insects that may be applied
sole or in mixtures with less toxic plant extracts to ascertain their complimentary attributes in
2
Chrysanthemum Flowers Lemongrass
Cut off and grind the flower heads Chop the lemongrass leaves and
using a mortar and pestle, after they stalks by using a pair of scissors,
are washed and air dried. after they are washed and air dried.
Add 300 ml of 95% Ethanol to the Add 300 ml of 95% Ethanol to the
ground chrysanthemum flowers chopped lemongrass leaves and
inside a container. stalks inside a container.
Let it soak for at least 24 hours. Let it soak for at least 24 hours.
Separate the ground flowers from the Separate the ground flowers from the
liquid mixturewith a strainer. liquid mixturewith a strainer.
Boil the mixture for 10-25 minutes. Boil the mixture for 10-25 minutes.
Refrigerate. Refrigerate.
Separate a portion of the extract and Separate a portion of the extract and
add 25% water to get 75% add 25% water to get 75%
Chrysanthemum Extract Chrysanthemum Extract
8
The Problem
The aim of this study is to determine the effectiveness of chrysanthemum flower and
1. What is the mortality rate of mosquitoes n the following trials with the use of
2. What is the mortality rate of mosquitoes in the following trials with the use of
lemongrass extract?
3. What is the mortality rate of mosquitoes in the following trials with the use of
lemongrass extract?
10
Research Hypothesis
in mitigating mosquitoes.
mitigating mosquitoes.
Homeowners - as the primary beneficiaries, they can use from the results of this study
to learn the health benefits of using organic products. They can also save money by creating
Community - This study can benefit the community since it can spread awareness of
the dangers of the constant use commercial insecticides and the promotion of using organic
Future Researchers - This study can help the next generation of researchers with the
same concerns. This can be used as a basis and reference to their own studies regarding
11
Scope and Delimitation
This study aims to test the effectiveness of chrysanthemum extract and lemongrass
extract as an alternative insecticide to help fight against mosquitoes. This is also focused on
the mosquito wrigglers that will be cultured and raised by the researchers.
The final product will be made and produced in the junior high school science lab
located at the Basic Education Department of Southwestern University PHINMA, Villa Aznar,
Urgello St., Cebu City, Cebu. The product will be tried and tested by the researchers on
mosquito larvae.
Research Methodology
This section presents the research design, research environment, research respondents,
Research Design
This study makes use of the experimental research design. Ary et al (2002) states that,
independent variables, controls any other relevant variables, and observes the effect of the
This type of research design is appropriate for this study, because the researchers aim
to produce a product that can work as an alternative insecticide and to record and study its
Research Environment
The experiment will be conducted at the junior high school science lab located at the
Basic Education Department of Southwestern University PHINMA, Villa Aznar, Urgello St.,
The respondents of this study will be the mosquito larvae that will be cultured and raised
by the researchers.
13
Research Subjects
mosquito larvae. Random Sampling Method will be used to gather the required data to verify
the level of effectiveness and mortality rate of the different concentrations of chrysanthemum
Research Materials
Chrysanthemum flowers
Lemongrass
95% Ethanol
Graduated cylinder
Beaker
Funnel
Measuring spoons
Strainer
Clean cloth
Scissors
Large container
Mosquito net
14
Research Procedure
The chrysanthemum flower heads were separated from the stems with a pair of
scissors and was ground with a mortar and pestle after it was washed and air dried.
300 ml of 95% Ethanol was poured on top of the 7.3 g of chrysanthemum flowers inside
an empty container. It was set aside for at least 24 hours. The ground chrysanthemum
flowers were separated from the extract with the use of a strainer. The extract was
The lemongrass was chopped with a pair of scissors after it was washed and air
dried. 300 ml of 95% Ethanol was poured on top of the 5.3 g of lemongrass inside an
empty container. It was set aside for at least 24 hours. The chopped lemongrass was
separated from the extract with the use of a strainer. The extract was boiled for 15-25
A large container was filled with water and set aside in a shaded area to entice
mosquitoes to freely lay their eggs. When the eggs are laid, the container will be
flower that contains natural insecticidal properties that can repel and kill insects.
75% Extract. Concentration that is 25% water and 75% chrysanthemum flower extract
or lemongrass extract.
50% Extract. Concentration that is 50% water and 50% chrysanthemum flower extract
or lemongrass extract.
16
CHAPTER II
This chapter includes the presentation of data, analysis and interpretation of data. The
tables present the mortality rate and level of effectiveness of the different concentrations of
EXTRACT EFFECTIVENESS
CONCENTRATIONS
100%
75%
50%
CHAPTER III
Summary of Findings
Conclusions
Recommendations
9
BIBLIOGRAPHY
A. BOOKS
Publishing Company
Publishing Company
Joy, P.P. et al. (2006). "Handbook of Herbs and Spices. Volume 3". England:
B. JOURNALS
Entomology Research.
the Pest Spodoptera Iittoralis Boisd and its Parasitoid Microplitis rufiventris Kok.
17
Amatobi, C.I. et al. (2006). "Botanical Pesticide Mixtures for Insect Pest Management on
Cowpea, Vigna unguiculata (L.) Walp Plants–The Legume Flower Bud Thrips,
Entomology Research
Entomology Research
Ethnopharmacology Research
cinerariaefolium Petals and Study its Activity Against Beetle Flour Tribolium
Baessler, L. (2018). "Organic Garden Pest Control: Using Chrysanthemum for Pest
https://www.gardeningknowhow.com/ornamental/flowers/chrysanthemum/chrysa
nthemum-pest-control.htm
Bauder, T. et al. (2018). "Pesticide Use Around the Home and Garden". Colorado State
https://extension.colostate.edu/docs/pubs/garden/xcm220.pdf
DeAngelis, J. (2013). "Pyrethrum and Permethrin Insecticide". Accessed June 22, 2019.
http://www.livingwithbugs.com/permethrin_pyrethrum.html
D'Incao, M.P. et al. (2013). "Phytochemicals taken from plants with potential in
Department of Health. (2018). “Monthly Dengue Report: Report No. 7”. Accessed
%20Monthly%20Report_MW1-MW30_2018_No.7.pdf
https://www.epa.gov/safepestcontrol.why-we-use-pesticides
Gimutao, K. & Salazar, F. (2018). "The Evolution of Entomological Research with Focus
focus-on-emerging-and-re-emerging-mosquito-borne-infect
Jackson, D. et al. (2008). "DEET General Fact Sheet". Accessed July 09, 2019.
http://npic.orst.edu/factsheets/DEETgen.html
https://pyrethrum.com/About_Pyrethrum/Pyrethrins_Pyrethroids.html
Morand, S. & Lajaunie, C. (2017). "Biodiversity and Health". Accessed July 23, 2019.
https://www.sciencedirect.com/topics/earth-and-planetary-sciences/insecticide
statistics
http://www.stuartxchange.com/RosasDeJapon.html
https://reliefweb.int/report/philippines/philippines-situation-report-7-dengue-
outbreak-13-september-2019
https://www.researchgate.net/publication/275959530_BASF_Insecticide_Mode_o
f_Action_Technical_Training_Manual
Sharif, Z. (2019). "5 Effective Pest Control Methods". Accessed July 23, 2019.
https://www.lifehack.org/539106/5-effective-pest-control-methods
2019. https://www.sigmaaldrich.com/life-science/nutrition-research/learning-
center/plant-profiler/cymbopogon.html
https://citybugs.tamu.edu/factsheets/ipm/ent-4002/
borne-disease
https://www.webmd.com/vitamins/ai/ingredientmono-904/chrysanthemum
Wells, J. (2017). "The Power of Lemongrass to Repel Mosquitoes and Biting Insects".
repel-mosquitoes-biting-insects/
BEBITA, AIBELL CHASCA ALONZO
PERSONAL INFORMATION
AGE: 17
NATIONALITY: FILIPINO
EDUCATIONAL BACKGROUND
S.Y. 2018-2020
ELEMENTARY:
BORCES, GLORY DAME
PERSONAL INFORMATION
AGE: 18
NATIONALITY: FILIPINO
EDUCATIONAL BACKGROUND
S.Y. 2018-2020
S. Y. 2014 - 2018
MINGLANILLA, CEBU
S. Y. 200 - 2014
CABURNAY, TRISSHA PEARL
CONTACT NUMBER:
EMAIL ADDRESS:
PERSONAL INFORMATION
AGE: 17
NATIONALITY: FILIPINO
RELIGION:
EDUCATIONAL BACKGROUND
S.Y. 2018-2020
S. Y. 2014 - 2018
S. Y. 200 – 2014
CASTELLANO, TRISHA MAE RAMIREZ
PERSONAL INFORMATION
AGE: 18
NATIONALITY: FILIPINO
EDUCATIONAL BACKGROUND
S.Y. 2018-2020
PERSONAL INFORMATION
AGE: 18
NATIONALITY: FILIPINO
EDUCATIONAL BACKGROUND
S.Y. 2018-2020
S.Y. 2014-2018
CONTACT NUMBER:
PERSONAL INFORMATION
AGE: 18
NATIONALITY: FILIPINO
RELIGION:
FATHER’S NAME:
EDUCATIONAL BACKGROUND
S.Y. 2018-2020
ELEMENTARY:
DELIMA, MERRY CONTESSA CASANDRA CANTIVEROS
PERSONAL INFORMATION
AGE: 17
NATIONALITY: FILIPINO
RELIGION:
EDUCATIONAL BACKGROUND
S.Y. 2018-2020
S.Y. 2014-2018
S.Y. 2008-2014
EBORDA, ROMEL ABACIAL
PERSONAL INFORMATION
AGE: 18
NATIONALITY: FILIPINO
RELIGION:
EDUCATIONAL BACKGROUND
S.Y. 2018-2020
S. Y. 2014 - 2018
S. Y. 200 - 2014
GUMAPAC, DWYNWEN MIASCO
PERSONAL INFORMATION
AGE: 17
NATIONALITY: FILIPINO
EDUCATIONAL BACKGROUND
S.Y. 2018-2020
S. Y. 2014 - 2018
S. Y. 200 - 2014