12 STEM Group 2

You might also like

Download as docx, pdf, or txt
Download as docx, pdf, or txt
You are on page 1of 43

CHRYSANTHEMUM FLOWER (Chrysanthemum cinerariifolium) AND LEMONGRASS

(Cymbopogon citratus) EXTRACT AS ALTERNATIVE INSECTICIDE AGAINST


MOSQUITO ()

___________________________

A Research Paper
Presented to
the Faculty of Senior High School Department
Southwestern University PHINMA
Cebu, City

___________________________

In Partial Fulfillment
of the Requirements for the Subject
Practical Research 2 (APP 006)

___________________________

By:

BEBITA, AIBELL CHASCA

BORCES, GLORY DAME

CABURNAY, TRISSHA PEARL

CASTELLANO, TRISHA MAE

COSTANILLA, MELANIE GRACE

CUYOS, ONE CARLOS MIGUEL

DELIMA, MERRY CONTESSA

EBORDA, ROMEL

GUMAPAC, DWYNWEN

iv
October 2019

APPROVAL SHEET

In partial fulfillment of the requirements for the subject PRACTICAL RESEARCH 2


(APP 006), this research paper entitled “CHRYSANTHEMUM FLOWER (Chrysanthemum
cinerariifolium) AND LEMONGRASS (Cymbopogon citratus) EXTRACT AS
ALTERNATIVE INSECTICIDE AGAINST MOSQUITO ()” prepared and submitted by AIBELL
CHASCA BEBITA, GLORY DAME BORCES, TRISSHA PEARL CABURNAY, TRISHA MAE
CASTELLANO, MELANIE GRACE COSTANILLA, ONE CARLOS MIGUEL CUYOS, MERRY
CONTESSA DELIMA, ROMEL EBORDA, and DWYNWEN GUMAPAC is hereby
recommended for PROPOSAL HEARING.

MELBIN A. DUCUSIN, LPT


Adviser

Approved in partial fulfillment of the requirements for the subject Practical Research 2
(APP 006) by the Proposal Hearing Committee

PATRICK M. SALINAS, MAED MELBIN A. DUCUSIN, LPT


Panel Panel

ACCEPTED in partial fulfillment of the requirements for the subject Practical Research 2
(APP 006).

PATRICK M. SALINAS, MAED


SHS Principal
Date of Proposal Hearing: August 13, 201
TABLE OF CONTENTS

TITLE PAGE ................................................................................................................................i

APPROVAL SHEET ...................................................................................................................ii

TABLE OF CONTENTS ............................................................................................................iii

CHAPTER I THE PROBLEM AND ITS SCOPE

A. Background of the Study .......................................................................................... 1

B. Review of Related Literature and Studies ................................................................ 3

C. Conceptual Framework ………………………………………………………..…….. 4

D. The Problem

a. Statement of the Problem.................................................................................. 8

b. Research Hypothesis...................................................................................... 9

c. Significance of the Study................................................................................ 9

d. Scope and Delimitation.................................................................................. 10

E. Research Methodology

a. Research Design............................................................................................... 11

b. Research Environment...................................................................................... 11

c. Research Subjects............................................................................................ 12

d. Research Materials ........................................................................................ 12

e. Research Procedure ................................................................................. 13

f. Statistical Treatment

F. Definition of Terms ............................................................................................. 14

v
CHAPTER II

PRESENTATION, ANALYSIS, AND INTERPRETATION OF DATA

CHAPTER III SUMMARY, FINDINGS, CONCLUSIONS & RECOMMENDATIONS

Summary

Findings

Conclusion

Recommendations

BIBLIOGRAPHY ..................................................................................................................... 15

RESEARCH DOCUMENTATION

APPENDICES ......................................................................................................................... 18

Questionnaire to Measure the Frequent Use of Chemical Insecticides ........................ 18

Questionnaire to Measure the Effectiveness of the Alternative Insecticide .................. 19

CURRICULUM VITAE

v
CHAPTER I

THE PROBLEM AND ITS SCOPE

Background of the Study

This section presents the important facts that support the validity of the data presented

in this research study.

The researchers are concerned of the harmful effects that pesticides cause as they are

used widely in agriculture, homes, schools, and other places all over the world. As well as the

fact that mosquito-borne diseases are getting worse every year, with dengue being the most

prominent disease in the Philippines.

In an article written by the Vector Disease Control International (VDCI) team (2019),

West Nile virus, another mosquito-borne disease, infected 49 out of 50 states in the USA and

the District of Columbia. A total of 2, 647 cases were reported and 167 confirmed deaths in the

year 2018.

According to the Philippines Department of Health’s (DOH) Monthly Dengue Report

(2018), dengue fever and dengue hemorrhagic fever are transmitted by the female Aedes

mosquito. They stated that a total of 69,088 dengue cases were reported nationwide from

January 1- July 28, 2018. In another report from reliefweb.int (2019), they mentioned that

WHO confirmed 271, 480 dengue cases, along with 720 deaths, were reported from January 1

– August 31, 2019. The results were 113% higher than in 2018. They also mentioned that the

Philippines Department of Health declared a National Dengue Epidemic on August 6, 2019.

The researchers came up with an idea to produce a safe and non-toxic pesticide that

can effectively mitigate mosquitoes and is free from chemicals and purely made with organic

ingredients and is also harmless to plants, animals, and humans.


Review of Related Literature and Studies

This section presents the works of different authors that are relevant to this study. It

contains quotations from various reference materials which were used as a basis for the

development and progress of this study.

Chrysanthemum (Chrysanthemum cinerariifolium) is a flowering plant; it's similar to

manzanilla, but taller. In the Philippines, the flower heads are usually white or various colored

(Philippine Medicinal Plants, 2018). It, especially the oil and extract, is usually used in

medicinal practices and making herbal medicine for its antibacterial and antibiotic properties.

(WebMD, 2018). These flowers are usually observed to study plant regeneration because of its

ability to grow branches when a part is cut off (Chen et al, 2019).

Chrysanthemums naturally produce a chemical called pyrethrin. Pyrethrin is a

neurotoxin that can kill insects but does not harm mammals or birds. According to MGK's

article 'Pyrethrins & Pyrethroids', pyrethrins are found at particularly high concentration

within flower structures known as achenes which are located in the flower head of the

Chrysanthemum. The extract from the Chrysanthemum plant containing pyrethrins is

called pyrethrum (Baessler, 2018).

Insecticidal properties of the flower genus Chrysanthemum are called Pyrethrum and

Pyrethrins. Chrysanthemum flowers are also called “insect flowers”. Pyrethrum is the approved

common name for dried flowers (Taplin, 1990).

As stated in Amal et al's study (2008), the extract from Chrysanthemum coronarium L.

and its fractions have shown insecticidal effect on cotton leaf worms. The flower extract

3
seemed to be a potential plant for application in integrated pest management because of its

safety to the environment.

In a similar study, researchers from the University of Baghdad in Iraq (Shawkat et al,

2011), extracted the crude active compound of pyrethrin to produce an insecticide made of

natural products as a substitute to synthetic ones. Different concentrations (20%, 30% and

40%) of the extract were used against flour beetles (Tribolium castanum).

Lemongrass (Cymbopogon citratus) is a fast growing tropical perennial plant that

contains aromatic oil. Its name is derived from the lemon-like odor of the essential oil present

in the stalks. Lemongrass oil is commonly used in culinary flavoring, medical practices and

even cosmetics (Joy et al, 2006).

Lemongrass is one of the commonly used plants in traditional medicinal practices in

most countries. In Brazilian folk medicine, it is used to treat nervous and gastrointestinal

disturbances, as well as feverish conditions. It is usually made into an infusion by pouring

boiling water on fresh or dried lemongrass leaves (this is infusion is called "abafado" in

Portuguese) and is orally ingested (Bueno et al, 1986)

Lemongrass oil is an effective insecticide against ticks, termites and dust mites and

various studies have shown lemongrass oil to be anywhere from 50 to 95% effective at

repelling insects for at least two to three hours according to study results as summarized in the

Sigma-Aldrich Plant Profiler (2010).

Lemongrass is also an effective insect repellent; it can repel various insects and

mosquitoes. Lemongrass is a natural repellent because of its high geraniol and citral content.

You can mix lemongrass oil with lemon eucalyptus oil for mosquito protection of about five to
six hours per application. You can also mix lemongrass oil with rosemary oil for a more

fragrant formula. This can ward off a greater number of insect species (Wells, 2017).

In addition to being used as a medicine, lemongrass can also be used in the food

industry, cosmetic industry and pharmaceutical industry as well. Ethanol extract from

lemongrass have shown anti-bacterial activity against Staphylococcus aureus and Salmonella

typhi. These ethanol extract contain protein, fats and carbohydrates (Nimrat et al, 2018).

Pests pose the serious threat to every living thing. There are many different kinds of

pests including fungus, mosquitoes, flies, rats, fleas, termites, feral dogs and any living

organism that has a harmful effect on humans, their surroundings, stock and food

supplies(Sharif, 2019).

There are approximately 3,000 different species of mosquitoes. Only the female

mosquitoes bite and suck blood because they need protein to help their eggs develop properly.

Mosquito-borne diseases are caused by the bacteria, viruses or parasites that were

transmitted by the mosquito when it is feeding. These are the most prominent mosquito-borne

diseases to date; Malaria, West Nile Virus, Yellow Fever, Dengue, Chikungunya, and Zika

Virus (Mosquito Reviews, 2019).

Of all the household pests in the world, mosquitoes are the most dangerous of them all.

Mosquito-transmitted diseases continue to cause massive problems to the public health

situation of tropical countries like the Philippines. Dengue, the world’s fastest-spreading

mosquito-borne disease, brought burden to a total of 131,827 Filipinos in 2017 (Gimutao &

Salazar, 2018).

5
Insecticides are types of pesticide that have chemical and biological origins. They are

used to control vectors, such as mosquitoes and ticks, which spread human and animal

diseases (Dettbarn et al, 2019).

According to 'Homeowner's Guide To: Pesticide Use Around the Home and Garden'

(Bauder et al, 2018), pests in the home and garden can cause damage to homes and

landscapes, health problems, and decrease the production of fruit and vegetables. The use of

pesticides is an option in aiding these serious issues. Nonetheless, if handled improperly,

pesticides can cause damage to our health and environment. Products that mitigate insects

(insecticides), weeds (herbicides), and fungi (fungicides) are pesticides. The components that

make these chemicals toxic to pests also can be harmful to people and animals. In some

cases, these products can also contaminate our water supplies.

Commercially available chemical pesticides are currently the most and only effective

means of pest control program. However, the frequent use of these chemical substances has

not only caused adverse effects on mammals’ health, but has also caused harmful impact on

the other beneficiary members of the ecosystem and the environment in which they are

immersed (Alagarmalai, 2017).

DEET, a chemical that is used in insect repelling products (e.g. sprays, lotions, etc.),

prevents bites from mosquitoes, biting flies, fleas and other small flying insects. It is a colorless

liquid with a faint odor and it does not dissolve easily in water. DEET was developed by the

U.S. Army in 1946 to protect soldiers from insect-infested areas. Since 1957, insect repellents

containing DEET have been used by the general public in the United States. It was estimated

that about 30% of the U.S. population uses one or more products that contain DEET every

year (Jackson et al, 2008).

6
According to Bloomquist (2015), pyrethroid insecticides are widely used compounds,

typically esters of chrysanthemic acid having a high degree of lipophilicity (fat solubility).  The

original compounds in this series were the natural pyrethrins (e.g., pyrethrin I), which are

isolated from the flowers of chrysanthemum. 

Pyrethroids were specifically designed to be more environmentally stable than

Pyrethrins. They provide long-lasting control and improved mammalian safety relative to other

products in use at the time they were developed. These compounds are commonly effective

against caterpillars, beetles, certain aphids and mites in crops, and for mosquito, termite and

cockroach control in non-crop segments (Salgado, 2013).

One alternative for insect pests control and to mitigate their attack is by using

phytochemicals. Naturally, many plant secondary metabolites (phytochemicals) affect insect

behavior, development and reproduction (Alagarmalai, 2017).

Chemical constituents present in plants have these activities and they show promise in

fighting pests in agriculture. An approach to these new insecticides that fulfill the requirements

of safety, efficacy and selectivity, can be by studying the defense mechanisms of plants.

Recent studies have shown the potential of insecticide substances and extracts isolated from

plants against various insect pests that cause serious damage to crops or stored grain. These

active ingredients are distributed by different plant organs and involved in secondary

metabolism. They are divided into three major groups: terpenes, phenols and nitrogenous

compounds (D'Incao et al, 2013).

Pesticides, especially insecticides, are used to control various pests and disease

carriers. Mosquitoes, ticks, rats and mice are some of the common targets. Vector-Borne

7
Diseases, Asthma and Allergies, Microbial Contamination are some examples of health

problems that are caused by pests (EPA, 2017).

Insecticides are highly effective emergency tools for Integrated Pest Management when

the insect pest population exceed economic threshold. Despite its advantages, insecticide use

has led to many environmental problems. Insecticide resistance from pests is caused by

natural selection (Ascoughll et al, 2008).

According to Merchant (2016), most consumers relate pesticides with pollution, health

risk, and toxic chemicals. About 75% of the consumers are wary of using pesticides. Extracts

(aqueous or oil) of some plant materials are toxic to some species of insect pests of crops

and others are less toxic. These extracts have lethal effects on insects that may be applied

sole or in mixtures with less toxic plant extracts to ascertain their complimentary attributes in

the management of pests (Amatobi et al, 2006).


Conceptual Framework

2
Chrysanthemum Flowers Lemongrass

Cut off and grind the flower heads Chop the lemongrass leaves and
using a mortar and pestle, after they stalks by using a pair of scissors,
are washed and air dried. after they are washed and air dried.

Add 300 ml of 95% Ethanol to the Add 300 ml of 95% Ethanol to the
ground chrysanthemum flowers chopped lemongrass leaves and
inside a container. stalks inside a container.

Let it soak for at least 24 hours. Let it soak for at least 24 hours.

Separate the ground flowers from the Separate the ground flowers from the
liquid mixturewith a strainer. liquid mixturewith a strainer.

Boil the mixture for 10-25 minutes. Boil the mixture for 10-25 minutes.

Refrigerate. Refrigerate.

100% Chrysanthemum Flower


100% Lemongrass Extract
Extract

Separate a portion of the extract and Separate a portion of the extract and
add 25% water to get 75% add 25% water to get 75%
Chrysanthemum Extract Chrysanthemum Extract

Separate another portion of the Separate another portion of the


extract and add 50% water to get extract and add 50% water to get
50% Chrysanthemum Extract 50% Chrysanthemum Extract
100%, 75%, 50%
Chrysanthemum Flower
Extract
Alternative Insecticide
Made from Chrysanthemum
Flowers and Lemongrass
Extracts

100%, 75%, 50%


Lemongrass Extract

Fig. 1: The Schematic Diagram of the Effectiveness of Chrysanthemum Extract and

Lemongrass Stalks as Alternative Insecticide

8
The Problem

Statement of the Problem

The aim of this study is to determine the effectiveness of chrysanthemum flower and

lemongrass extract as an alternative insecticide.

This also aims to answer these specific questions:

1. What is the mortality rate of mosquitoes n the following trials with the use of

chrysanthemum flower extract?

1.1. 100% pure concentration

1.2. 75% concentration

1.3. 50% concentration

2. What is the mortality rate of mosquitoes in the following trials with the use of

lemongrass extract?

2.1. 100% pure concentration

2.2. 75% concentration

2.3. 50% concentration

3. What is the mortality rate of mosquitoes in the following trials with the use of

50/50 ratio of chrysanthemum flower extract and lemongrass extract?

4. Is there a significant difference between the different concentrations of

chrysanthemum flower extract

5. Is there a significant difference between the different concentrations of

lemongrass extract?

6. What is the level of effectiveness of the chrysanthemum flower and lemongrass

extract on mitigating mosquitoes?

10
Research Hypothesis

1. There is no significant difference in mosquito mortality rate between the different

concentrations of chrysanthemum flower extract and lemongrass extract.

2. There is a significant difference in mosquito mortality rate between the different

concentrations of chrysanthemum flower extract and lemongrass extract.

3. There is no significant difference between the level of effectiveness of the

different concentrations of chrysanthemum flower extract and lemongrass extract

in mitigating mosquitoes.

4. There is a significant difference between the level of effectiveness of the different

concentrations of chrysanthemum flower extract and lemongrass extract in

mitigating mosquitoes.

Significance of the Study

The results of the study will benefit the following:

Homeowners - as the primary beneficiaries, they can use from the results of this study

to learn the health benefits of using organic products. They can also save money by creating

their own products that are made of organic ingredients.

Community - This study can benefit the community since it can spread awareness of

the dangers of the constant use commercial insecticides and the promotion of using organic

products that are non-toxic to humans and the environment.

Future Researchers - This study can help the next generation of researchers with the

same concerns. This can be used as a basis and reference to their own studies regarding

insecticides and the use of organic ingredients.

11
Scope and Delimitation

This study aims to test the effectiveness of chrysanthemum extract and lemongrass

extract as an alternative insecticide to help fight against mosquitoes. This is also focused on

the mosquito wrigglers that will be cultured and raised by the researchers.

The final product will be made and produced in the junior high school science lab

located at the Basic Education Department of Southwestern University PHINMA, Villa Aznar,

Urgello St., Cebu City, Cebu. The product will be tried and tested by the researchers on

mosquito larvae.
Research Methodology

This section presents the research design, research environment, research respondents,

research materials, research procedure of the study.

Research Design

This study makes use of the experimental research design. Ary et al (2002) states that,

"Experimental is a scientific investigation in which the researcher manipulates one or more

independent variables, controls any other relevant variables, and observes the effect of the

manipulations on the dependent variables."

This type of research design is appropriate for this study, because the researchers aim

to produce a product that can work as an alternative insecticide and to record and study its

effectiveness against mosquitoes.

Research Environment

The experiment will be conducted at the junior high school science lab located at the

Basic Education Department of Southwestern University PHINMA, Villa Aznar, Urgello St.,

Cebu City, Cebu.

The respondents of this study will be the mosquito larvae that will be cultured and raised

by the researchers.

13
Research Subjects

The researchers will test the different concentrations on a significant number of

mosquito larvae. Random Sampling Method will be used to gather the required data to verify

the level of effectiveness and mortality rate of the different concentrations of chrysanthemum

flower and lemongrass extract on mitigating mosquitoes.

Research Materials

 Chrysanthemum flowers

 Lemongrass

 95% Ethanol

 Mortar and pestle

 Graduated cylinder

 Beaker

 Funnel

 Measuring spoons

 Strainer

 Clean cloth

 Scissors

 (7) Empty spray bottle

 (7) Empty containers

 Large container

 Mosquito net

14
Research Procedure

1. Preparation and Extraction of Chrysanthemum Flowers

The chrysanthemum flower heads were separated from the stems with a pair of

scissors and was ground with a mortar and pestle after it was washed and air dried.

300 ml of 95% Ethanol was poured on top of the 7.3 g of chrysanthemum flowers inside

an empty container. It was set aside for at least 24 hours. The ground chrysanthemum

flowers were separated from the extract with the use of a strainer. The extract was

boiled for 15-25 minutes. Then it was refrigerated.

2. Preparation and Extraction of Lemongrass

The lemongrass was chopped with a pair of scissors after it was washed and air

dried. 300 ml of 95% Ethanol was poured on top of the 5.3 g of lemongrass inside an

empty container. It was set aside for at least 24 hours. The chopped lemongrass was

separated from the extract with the use of a strainer. The extract was boiled for 15-25

minutes. Then it was refrigerated.

3. Raising Mosquito Larvae

A large container was filled with water and set aside in a shaded area to entice

mosquitoes to freely lay their eggs. When the eggs are laid, the container will be

covered with a net.


Definition of Terms

The following terms are defined operationally:

Chrysanthemum Flower Extract. It is an independent of this study. It is a type of

flower that contains natural insecticidal properties that can repel and kill insects.

Lemongrass Extract. It is the other independent variable of this study. It is a medical

and culinary herb which contains natural insect repelling properties.

Insecticide. A type of pesticide that is only focused on mitigating insects (e.g.

mosquitoes, flies, cockroaches, etc.).

100% Pure Extract. Concentration of chrysanthemum flower extract or lemongrass

extract without the addition of water.

75% Extract. Concentration that is 25% water and 75% chrysanthemum flower extract

or lemongrass extract.

50% Extract. Concentration that is 50% water and 50% chrysanthemum flower extract

or lemongrass extract.

16
CHAPTER II

PRESENTATION, ANALYSIS, AND INTERPRETATION OF DATA

This chapter includes the presentation of data, analysis and interpretation of data. The

tables present the mortality rate and level of effectiveness of the different concentrations of

chrysanthemum flower and lemongrass extract against mosquitoes.

CHRYSANTHEMUM SAMPLE VOLUME MORTALITY RATE LEVEL OF

EXTRACT EFFECTIVENESS

CONCENTRATIONS

100%

75%

50%
CHAPTER III

SUMMARY OF FINDINGS, CONCLUSIONS, AND RECOMMENDATIONS

Summary of Findings

Conclusions

Recommendations
9
BIBLIOGRAPHY

A. BOOKS

Ary, D. et al. (2002). "Introduction to Research. 6th Edition". Belmont: Wadsworth

Publishing Company

Ascoughll, J. C. et al. (2008). “Encyclopedia of Ecology”. Amsterdam: Elsevier

Publishing Company

Dettbarn, W. D. et al. (2019). "Biomarkers in Toxicology. 2nd Edition". Amsterdam:

Elsevier Publishing Company

Gimutao, K. & Salazar, F. (2018). "The Evolution of Entomological Research with

Focus on Emerging and Re-emerging Mosquito-Borne Infections in the

Philippines". London: IntechOpen Limited

Joy, P.P. et al. (2006). "Handbook of Herbs and Spices. Volume 3". England:

Woodhead Publishing Limited

B. JOURNALS

Alagarmalai, J. (2017). "Phytochemicals: As Alternate to Chemical Pesticides for Insects

Pest Management. Current Trends Biomedical Engineering & Bioscience".

Entomology Research.

Amal, A. et al. (2008). "Insecticidal Effect of Chrysanthemum coronarium L. Flowers on

the Pest Spodoptera Iittoralis Boisd and its Parasitoid Microplitis rufiventris Kok.

with Identifying the Chemical Composition." Applied Science Research

17
Amatobi, C.I. et al. (2006). "Botanical Pesticide Mixtures for Insect Pest Management on

Cowpea, Vigna unguiculata (L.) Walp Plants–The Legume Flower Bud Thrips,

Megalurothrips sjostedti Trybom. Journal of Sustainable Agriculture".

Entomology Research

Bloomquist, J. (2015). "Insecticides: Chemistries and Characteristics 2nd Edition".

Entomology Research

Bueno, O. et al. (1986). "Pharmacology of Lemongrass (Cymbopogon citratus Stapf). I.

Effects of Teas prepared from the Leaves on Laboratory Animals".

Ethnopharmacology Research

Chen, F. et al. (2019). "The Genetics of Planting Density-Dependent Branching in

Chrysanthemum". Horticulture Research

Nimrat, S. et al. (2018). “Chemical Composition and Antibacterial Activity of Ethanol

Extract Of Lemongrass (Cymbopogon citratus) on Pathogenic Bacteria: A

review”. Entomology Research

Shawkat, M. et al. (2011). "Extraction of Pyrethrins from Chrysanthemum

cinerariaefolium Petals and Study its Activity Against Beetle Flour Tribolium

castanum". Biotechnology Research


C. ELECTRONIC SOURCES

Baessler, L. (2018). "Organic Garden Pest Control: Using Chrysanthemum for Pest

Control". Accessed June 29, 2019.

https://www.gardeningknowhow.com/ornamental/flowers/chrysanthemum/chrysa

nthemum-pest-control.htm

Bauder, T. et al. (2018). "Pesticide Use Around the Home and Garden". Colorado State

University Extension. Accessed June 29, 2019.

https://extension.colostate.edu/docs/pubs/garden/xcm220.pdf

DeAngelis, J. (2013). "Pyrethrum and Permethrin Insecticide". Accessed June 22, 2019.

http://www.livingwithbugs.com/permethrin_pyrethrum.html

D'Incao, M.P. et al. (2013). "Phytochemicals taken from plants with potential in

management of Spodoptera frugiperda (Lepidoptera: Noctuidae)". Accessed

June 28, 2019. http://www.jbiopest.com/users/LW8/efiles/Vol_6_2_182-192.pdf

Department of Health. (2018). “Monthly Dengue Report: Report No. 7”. Accessed

September 1, 2019. https://www.doh.gov.ph/sites/default/files/statistics/Dengue

%20Monthly%20Report_MW1-MW30_2018_No.7.pdf

Environmental Protection Agency (EPA). (2017). “Why We Use Pesticides”.

https://www.epa.gov/safepestcontrol.why-we-use-pesticides

Gimutao, K. & Salazar, F. (2018). "The Evolution of Entomological Research with Focus

on Emerging and Re-emerging Mosquito-Borne Infections in the Philippines".

Accessed July 23, 2019. https://www.intechopen.com/books/public-health-


emerging-and-re-emerging-issues/the-evolution-of-entomological-research-with-

focus-on-emerging-and-re-emerging-mosquito-borne-infect

Jackson, D. et al. (2008). "DEET General Fact Sheet". Accessed July 09, 2019.

http://npic.orst.edu/factsheets/DEETgen.html

McLaughlin Gormley King Company (MGK). (2010). "Pyrethrins & Pyrethroids".

Accessed June 29, 2019.

https://pyrethrum.com/About_Pyrethrum/Pyrethrins_Pyrethroids.html

Morand, S. & Lajaunie, C. (2017). "Biodiversity and Health". Accessed July 23, 2019.

https://www.sciencedirect.com/topics/earth-and-planetary-sciences/insecticide

Mosquito Reviews. (2019). "Statistics for Mosquito-Borne Diseases and Death".

Accessed August 23, 2019. https://mosquitoreviews.com/learn/disease-death-

statistics

Philippine Medicinal Plants. (2018). "Rosas de Japon". Accessed August 2, 2019.

http://www.stuartxchange.com/RosasDeJapon.html

ReliefWeb. (2019). “Philippines Situation Report 7 – Dengue Outbreak (13 September

2019”. Accessed September 1, 2019

https://reliefweb.int/report/philippines/philippines-situation-report-7-dengue-

outbreak-13-september-2019

Salgado, V. (2013). "BASF Insecticide Mode of Action: Technical Training Manual".

Accessed July 07, 2019.

https://www.researchgate.net/publication/275959530_BASF_Insecticide_Mode_o

f_Action_Technical_Training_Manual
Sharif, Z. (2019). "5 Effective Pest Control Methods". Accessed July 23, 2019.

https://www.lifehack.org/539106/5-effective-pest-control-methods

Sigma-Aldrich Plant Profiler. (2010). "Lemongrass (Cymbopogon)". Accessed June 30,

2019. https://www.sigmaaldrich.com/life-science/nutrition-research/learning-

center/plant-profiler/cymbopogon.html

Merchant, M. (August 9, 2016). "Understanding Common House and Garden

Insecticides". Accessed June 22, 2019.

https://citybugs.tamu.edu/factsheets/ipm/ent-4002/

VDCI. (August 1, 2019). “2018 Mosquito-borne Disease Year in Review”. Accessed

September 1, 2019. http://www.vdci.net/blog/2018-year-in-review-mosquito-

borne-disease

WebMD. (2018). "CHRYSANTHEMUM". Accessed August 1, 2019.

https://www.webmd.com/vitamins/ai/ingredientmono-904/chrysanthemum

Wells, J. (2017). "The Power of Lemongrass to Repel Mosquitoes and Biting Insects".

Accessed July 09, 2019. http://mosquitorepellentinsider.com/power-lemongrass-

repel-mosquitoes-biting-insects/
BEBITA, AIBELL CHASCA ALONZO

LABOGON, MANDAUE CITY, CEBU

CONTACT NUMBER: 09296595931

EMAIL ADDRESS: ebellebell504@gmail.com

PERSONAL INFORMATION

AGE: 17

BIRTHDATE: JANUARY 15, 2002

BIRTHPLACE: LAPU-LAPU CITY, CEBU

NATIONALITY: FILIPINO

CIVIL STATUS: SINGLE

RELIGION: ROMAN CATHOLIC

FATHER’S NAME: PHILIP REY BEBITA SR.

MOTHER’S MAIDEN NAME: ANGELI ALONZO

EDUCATIONAL BACKGROUND

SENIOR HIOGH SCHOOL: SOUTHWESTERN UNIVERSITY PHINMA

VILLA AZNAR, URGELLO ST., CEBU CITY. CEBU

S.Y. 2018-2020

JUNIOR HIGH SCHOOL:

ELEMENTARY:
BORCES, GLORY DAME

TUYAN, NAGA CITY, CEBU

CONTACT NUMBER: 09154657011

EMAIL ADDRESS: glory.borces@gmail.com

PERSONAL INFORMATION

AGE: 18

BIRTHDATE: AUGUST 09, 2001

BIRTHPLACE: PAGADIAN CITY

NATIONALITY: FILIPINO

CIVIL STATUS: SINGLE

RELIGION: ROMAN CATHOLIC

FATHER’S NAME: DANILO BORCES

MOTHER’S MAIDEN NAME: MELANIE

EDUCATIONAL BACKGROUND

SENIOR HIGH SHOOL: SOUTHWESTERN UNIVERSITY PHINMA

VILLA AZNAR, URGELLO ST., CEBU CITY. CEBU

S.Y. 2018-2020

JUNIOR HIGH SCHOOL: NAGA NATIONAL HIGH SCHOOL

NAGA CITY, CEBU

S. Y. 2014 - 2018

ELEMENTARY: UNIVERSITY OF VISAYAS - MINGLANILLA CAMPUS

MINGLANILLA, CEBU

S. Y. 200 - 2014
CABURNAY, TRISSHA PEARL

M.H. DEL PILAR ST., GUIZO, MANDAUE CITY, CEBU

CONTACT NUMBER:

EMAIL ADDRESS:

PERSONAL INFORMATION

AGE: 17

BIRTHDATE: OCTOBER 05, 2001

BIRTHPLACE: CEBU CITY

NATIONALITY: FILIPINO

CIVIL STATUS: SINGLE

RELIGION:

FATHER’S NAME: JOSE PERCIVAL CABURNAY

MOTHER’S MAIDEN NAME: IVY

EDUCATIONAL BACKGROUND

SENIOR HIGH SCHOOL: SOUTHWESTERN UNIVERSITY PHINMA

VILLA AZNAR, URGELLO ST., CEBU CITY. CEBU

S.Y. 2018-2020

SECONDARY: ST. LOUIS COLLEGE OF CEBU

SUDLON, MAGUIKAY, MANDAUE CITY, CEBU

S. Y. 2014 - 2018

PRIMARY: MAGUIKAY ELEMENTARY SCHOOL

MAGUIKAY, MANDAUE CITY, CEBU

S. Y. 200 – 2014
CASTELLANO, TRISHA MAE RAMIREZ

SOUTH HILLS SUBDIVISION, TISA, CEBU CITY, CEBU

CONTACT NUMBER: 09957530948

EMAIL ADDRESS: trishacastellano@gmail.com

PERSONAL INFORMATION

AGE: 18

BIRTHDATE: MAY 02, 2001

BIRTHPLACE: CEBU CITY, CEBU

NATIONALITY: FILIPINO

CIVIL STATUS: SINGLE

RELIGION: ROMAN CATHOLIC

FATHER’S NAME: REY STEPHEN CASTELLANO

MOTHER’S MAIDEN NAME: ARMYNNE RAMIREZ

EDUCATIONAL BACKGROUND

SENIOR HIGH SCHOOL: SOUTHWESTERN UNIVERSITY PHINMA

VILLA AZNAR, URGELLO ST., CEBU CITY. CEBU

S.Y. 2018-2020

SECONDARY: UNIVERSITY OF SAN CARLOS – BED - SC

J. ALCANTARA ST., CEBU CITY, CEBU

S.Y. 2014 - 2018

PRIMARY: UNIVERSITY OF SAN CARLOS – BED - SC

J. ALCANTARA ST., CEBU CITY, CEBU

S.Y. 200 - 2014


COSTANILLA, MELANIE GRACE EDPAN

BRGY. TUNGKIL, MINGLANILLA, CEBU

CONTACT NUMBER: 09432952631

EMAIL ADDRESS: melcos0101@gmail.com

PERSONAL INFORMATION

AGE: 18

BIRTHDATE: JANUARY 01, 2001

BIRTHPLACE: NAGA CITY, CEBU

NATIONALITY: FILIPINO

CIVIL STATUS: SINGLE

RELIGION: SEVENTH-DAY ADVENTIST

FATHER’S NAME: BEDEVER A. COSTANILLA

MOTHER’S MAIDEN NAME: CHEERY GLADYS N. EDPAN

EDUCATIONAL BACKGROUND

SENIOR HIHGH SCHOOL: SOUTHWESTERN UNIVERSITY PHINMA

VILLA AZNAR, URGELLO ST., CEBU CITY. CEBU

S.Y. 2018-2020

JUNIOR HIGH SCHOOL: SOUTHWESTERN UNIVERSITY PHINMA

VILLA AZNAR, URGELLO ST., CEBU CITY. CEBU

S.Y. 2014-2018

ELEMENTARY: CEBU CITY SDA ELEMENTARY SCHOOL

TRES DE ABRIL ST. EXT., CEBU CITY, CEBU

S.Y. 2010 – 2014


CUYOS, ONE CARLOS MIGUEL RAGA

ENGLIS, V. RAMA, CEBU CITY

CONTACT NUMBER:

EMAIL ADDRESS: onecarlos629@yahoo.com

PERSONAL INFORMATION

AGE: 18

BIRTHDATE: JUNE 29,2001

BIRTHPLACE: CEBU CITY

ADDRESS: ENGLIS V, RAMA CEBU CITY

NATIONALITY: FILIPINO

CIVIL STATUS: SINGLE

RELIGION:

FATHER’S NAME:

MOTHER’S MAIDEN NAME:

EDUCATIONAL BACKGROUND

SENIOR HIGH SCHOOL: SOUTHWESTERN UNIVERSITY PHINMA

VILLA AZNAR, URGELLO ST., CEBU CITY. CEBU

S.Y. 2018-2020

JUNIOR HIGH SCHOOL:

ELEMENTARY:
DELIMA, MERRY CONTESSA CASANDRA CANTIVEROS

172 V.RAMA AVE. CEBU CITY, CEBU

CONTACT NUMBER: 09565971083

EMAIL ADDRESS: merrycantiverosdelima@gmail.com

PERSONAL INFORMATION

AGE: 17

BIRTHDATE: NOVEMBER 3, 2001

BIRTHPLACE: MINGLANILLA DISTRICT HOSPITAL

NATIONALITY: FILIPINO

CIVIL STATUS: SINGLE

RELIGION:

FATHER’S NAME: ROLANDO Q. DELIMA JR.

MOTHER’S MAIDEN NAME: LYNETTE P. CANTIVEROS

EDUCATIONAL BACKGROUND

SENIOR HIGH SCHOOL: SOUTHWESTERN UNIVERSITY PHINMA

VILLA AZNAR, URGELLO ST., CEBU CITY. CEBU

S.Y. 2018-2020

JUNIOR HIGH SCHOOL: ABELLANA NATIONAL SCHOOL

OSMENA BLVD., CEBU CITY

S.Y. 2014-2018

ELEMENTARY: CEBU CITY CENTRAL SCHOOL

P. DEL ROSARIO EXT. CEBU CITY

S.Y. 2008-2014
EBORDA, ROMEL ABACIAL

LANGUB, KALUNASAN, CEBU CITY, CEBU

CONTACT NUMBER: 09774340571

EMAIL ADDRESS: romeleborda2018@gmail.com

PERSONAL INFORMATION

AGE: 18

BIRTHDATE: JULY 09, 2001

BIRTHPLACE: CEBU CITY, CEBU

NATIONALITY: FILIPINO

CIVIL STATUS: SINGLE

RELIGION:

FATHER’S NAME: ROMEO EBORDA

MOTHER’S MAIDEN NAME: MARICEL A. ABACIAL

EDUCATIONAL BACKGROUND

SENIOR HIGH SCHOOL: SOUTHWESTERN UNIVERSITY PHINMA

VILLA AZNAR, URGELLO ST., CEBU CITY. CEBU

S.Y. 2018-2020

JUNIOR HIGH SCHOOL: RAMON DUTERTE MEMORIAL NATIONAL HIGH SCHOOL

GUADALUPE, CEBU CITY, CEBU

S. Y. 2014 - 2018

ELEMENTARY: GUADALUPE ELEMENTARY SCHOOL

GUADALUPE, CEBU CITY, CEBU

S. Y. 200 - 2014
GUMAPAC, DWYNWEN MIASCO

22 G. B. RODRIGUEZ ST., SAMBAG 2, CEBU CITY, CEBU

CONTACT NUMBER: 09667355794

EMAIL ADDRESS: dwynwenm@gmail.com

PERSONAL INFORMATION

AGE: 17

BIRTHDATE: MARCH 11, 2002

BIRTHPLACE: CEBU CITY, CEBU

NATIONALITY: FILIPINO

CIVIL STATUS: SINGLE

RELIGION: ROMAN CATHOLIC

FATHER’S NAME: PEDRO GUMAPAC

MOTHER’S MAIDEN NAME: GUENEVERE MIASCO

EDUCATIONAL BACKGROUND

SENIOR HIGH SCHOOL: SOUTHWESTERN UNIVERSITY PHINMA

VILLA AZNAR, URGELLO ST., CEBU CITY. CEBU

S.Y. 2018-2020

JUNIOR HIGH SCHOOL: ST. THERESA’S COLLEGE OF CEBU

DON RAMON ABOITIZ ST., CEBU

S. Y. 2014 - 2018

ELEMENTARY: LA HACIENDA ELEMENTARY SCHOOL

LA HACIENDA, ALICIA, BOHOL

S. Y. 200 - 2014

You might also like