Service - Manual Medonic 520

MEDONIC CELLANALYZER CA530 Version 2.
0 Page : 1
Sweden SERVICE MANUAL Date : 20-11-24
-----------------------------------------------------------------------------------------------------------------------------------
0 CONTENTS
0 CONTENTS.............................................................................................................................................................................1
0.1 COPYRIGHT () NOTICE....................................................................................................................................... 3
0.2 SERVICEMANUAL CHANGES/UPDATES.............................................................................................................. 4
0.3 ERRATA.................................................................................................................................................................... 4
1 FUNCTIONAL DESCR. FLUIDIC SYSTEM.....................................................................................................................5
1.1 INTRODUCTION FLUIDIC DIAGRAM / GLOSSARY............................................................................................ 5
1.2 WHOLE BLOOD ASPIRATION................................................................................................................................ 9
1.3 FIRST DILUTION.................................................................................................................................................... 10
1.4 MIXING THE FIRST DILUTION............................................................................................................................ 11
1.5 PREPARING THE FINAL DILUTION.................................................................................................................... 12
1.6 CLEANING THE ASPIRATION PIPETTE.............................................................................................................. 13
1.7 FINAL DILUTION................................................................................................................................................... 14
1.8 START ANALYZING PROCESS............................................................................................................................ 15
1.9 CLEANING THE MEASURING CHAMBERS........................................................................................................ 16
1.10 FINAL POSITION.................................................................................................................................................. 17
2.0 PREDILUTED SAMPLES FLUID DIAGRAM................................................................................................................18
3 SERVICE MENU’S / SPECIAL PROCEDURES.............................................................................................................19

3.1 VALVE / WASTE-PUMP CHECK........................................................................................................................... 19
3.2 PHOTOMETER CHECK / ADJUSTMENT.............................................................................................................. 19
3.3 START / STOP DETECTOR CHECK / ADJUSTMENT..........................................................................................22
3.4 BOTTLE DETECTORS & BLOOD DETECTOR ADJUSTMENT..........................................................................24
3.5 TURNING VALVE & PISTON DETECTOR CHECK.............................................................................................24
3.6 MAINS SUPPLY NOISE TEST................................................................................................................................ 25
3.7 TURNING VALVE ADJUSTMENT........................................................................................................................ 26
3.8 PISTON ADJUSTMENT.......................................................................................................................................... 29
3.9 “ PRINT ALL SETTINGS”................................................................................................................................... 31
3.10 “ PRINT MACHINE STATISTICS”....................................................................................................................... 33
4 INDICATION NUMBER ### DISPLAYED...................................................................................................................37
4.1 INDICATION 1-99................................................................................................................................................... 37
4.2 INDICATION 100-199............................................................................................................................................. 37
4.3 INDICATION 200-299............................................................................................................................................. 40
4.4 INDICATION 300-399............................................................................................................................................. 42
4.4 INDICATION 900-999............................................................................................................................................. 43
4.5 INDICATION 1000-1999......................................................................................................................................... 44
4.6 INDICATION 2000-2999......................................................................................................................................... 45
4.7 CPU / SYSTEM BOOTING ERRORS ( BEEP’ S)....................................................................................................46
5 CHANGING/INSTALLING PARTS , PC-BOARDS ETC..............................................................................................47
5.1 CHANGING PC-BOARDS...................................................................................................................................... 47
5.2 CHANGING PARTS / TURNING VALVES............................................................................................................ 48
5.3 CHANGING THE SYRINGE/PISTON..................................................................................................................... 49
5.4 CHANGING VALVES & TUBES............................................................................................................................ 51
5.5 CHANGING THE ORIFICE TRANSDUCER........................................................................................................... 52
5.6 CHANGING / CLEANING THE MIXING CUP....................................................................................................... 53
5.7 CHANGING THE VIEWING ANGLE (LCD DISPLAY).........................................................................................53
5.7 CHANGE OF PISTON ( ROD ) SEALINGS............................................................................................................. 54
MEDONIC CELLANALYZER CA530 Version 2.0 Page : 2
-----------------------------------------------------------------------------------------------------------------------------------
6 TUBING SYSTEM LAY-OUT...........................................................................................................................................57

6.1 LEGENDS................................................................................................................................................................ 57
7 AUXILIARY DEVICES.......................................................................................................................................................58
7.1 INSTALLING THE PRINTER AND SERIAL OUTPUT..........................................................................................58
7.2 SYSTEM STATUS LOGGING................................................................................................................................. 59
7.3 BAR_CODE SCANNER.......................................................................................................................................... 59
7.4 CAP PIERCING DEVICE CT220.......................................................................................................................... 59
7.4.1 ADJUSTMENT MICRO_SWITCHES......................................................................................................................................60
7.4.2 ADJUSTING THE BLOOD SWITCH......................................................................................................................................63
7.4.3. TUBING CONNECTION.....................................................................................................................................................65
7.5 MICRO CAPILLARY ADAPTER AD260................................................................................................................ 66
7.6 INSTALLING UPS / MAINS STABILIZERS........................................................................................................... 67
8 PARAMETER FAILURE ANALYSIS..............................................................................................................................68
8.1 HGB INSTABILITY................................................................................................................................................. 68
8.2 MCV INSTABILITY................................................................................................................................................ 69
8.3 WBC INSTABILITY................................................................................................................................................ 69
8.4 RBC INSTABILITY................................................................................................................................................. 70
8.5 PLT INSTABILITY.................................................................................................................................................. 70
9 DESCRIPTION ELECTRONICS.......................................................................................................................................71
9.1 CPU BOARD 530-9001............................................................................................................................................ 71
9.2 POWER-SUPPLY BOARD 530-9011....................................................................................................................... 74
9.3 ANALOGUE AMPLIFIER BOARD 530-9021........................................................................................................ 76
9.4 DISPLAY / KEY-BOARD 530-9032....................................................................................................................... 77
9.5 INPUT MAINS FILTER BOARD 530-9040............................................................................................................ 77
11 SERVICE SCHEDULE........................................................................................................................................................80
11.1 SERVICE POINTS............................................................................................................................................... 81
12 APPENDIX / TECHNICAL BULLETINS........................................................................................................................85
13 REPORTING SERVICE QUESTIONS.............................................................................................................................86
14 ORDERING SPARE PARTS..............................................................................................................................................87

-----------------------------------------------------------------------------------------------------------------------------------
0.1 COPYRIGHT () NOTICE
PLEASE NOTE :
All rights strictly reserved. Reproduction or issue of this

manual or parts of it to third parties in any form whatever
is not permitted without written authority from the
proprietors MEDONIC in Sweden.
This copy issued by :
Date :
-----------------------------------------------------------------------------------------------------------------------------------
0.2 SERVICEMANUAL CHANGES/UPDATES
This service manual is based on Program Version 1.88 and hardware release serial #
2000-2025 and > 2026. From serial 2026 , the CPU PCB has a version number 530-700-2 which
can be used with the Panasonic / Matsushita ZE-84RMSM Bar-Code scanner.
Refer to section 12 in this manual for latest info regarding the CA530 technical maintenance and
software updates.
Filename of this service manual : 530-31-200

Appendix numbering: 530-31-201 to 530-31-299
Filename of user manual : 530-30-200

Appendix numbering: 530-30-201 to 530-30-299
Section 12 :
Document 530-31-201 Software update history

530.31.203 On request only
530-31-209 CA620 update
530.43.7 Optical piston detector adjustment
530.43.8 AD260 installation
000-80-014 MAX122 service report
0.3 ERRATA
This section is reserved for errata corrections.

Please report errors in this manual to : mail@medonic.se ( Attention: Technical Dept. )
-----------------------------------------------------------------------------------------------------------------------------------
1 FUNCTIONAL DESCR. FLUIDIC SYSTEM
1.1 INTRODUCTION FLUIDIC DIAGRAM / GLOSSARY
The CA530 performs two dilution’s, 1:400 and 1:40.000 . The 1:400 dilution is used for the WBC
and HGB determination and the 1:40.000 dilution is used to perform the RBC and PLT
measurements. These dilution’s are done in 2 separate stages. The first and second dilution stages
are completely separated from each other . One turning valve is used with 2 separated micro
channels of 25 ul each .
Fig 1 shows the complete fluidic diagram with the CA530 in it’s home position. To understand it’s
functions, please refer first to the symbol glossary below .
-----------------------------------------------------------------------------------------------------------------------------------
Glossary
Valves
Pinch valves are of 2 types.
A single valve is a normally closed valve and has the symbol :
Fig. 1A
A two-way valve has a normally closed and a normally open section.

The normally open section is marked as A and the normally closed as B
B
Fig. 1B
Turning valve
Position 'A' Position 'B'
3 3
1 1
2 2 2 2
1 3 1 3
Fig. 1C
-----------------------------------------------------------------------------------------------------------------------------------
The turning valve has two separated 25 ul channels. In the figure above, the position ‘A’ is drawn
in it’s home position. Position 1 and position 2 are open and 3 closed.
If the valve is turned 45 degrees clockwise, position 2 and 3 will be open and position 1 closed.
Called ‘Position B’ in the description below.
The microvolume at pos. 1 is used for whole blood only and the volume as drawn at pos. 2 for the
diluted sample.
The turning valve itself is surrounded by diluent in a closed / sealed compartment.
Syringe / measuring chamber
Rod
Diluent /
B 9 A Lyser
Piston
R Measuring
B Chamber
C
Orifice
Cap.
16 12
A 15 B
Fig 1D
The measuring chamber and the diluter are combined in one block. The piston is driven by a rod
and acts therefore as a displacement and as a conventional syringe simultaneously.
i.e. A movement of the piston will always create a larger volume displacement in the position
below the piston ( = measuring chamber).
Valve 9 acts as an in/out valve for the diluent (or lyser with valve 10) . Valve 12 is connected to the
open air for evacuation purposes and valve 15 is the waste outlet control.
The orifice is integrated in the cylinder and has 2 inlets, whereof one is connected via valve 16 to
the measuring chamber.
Both cylinders in the system are basically identical. One is used for the RBC / PLT count and the
second for the WBC / HGB determination.
-----------------------------------------------------------------------------------------------------------------------------------
Mixing cup
A
1 2 3 4
B
Mix Cup
Fig. 1E
The mixing cup is used to collect the first internal dilution of ca. 1 : 200 and is hermetically closed
with an O-ring. Inlets and/or outlets are marked as 1 to 4.
Inlet 1 is used to create vacuum . Inlet 2 is used for the inlet of the first dilution. ( 25 ul blood and
ca. 5.2 ml diluent ). The center pin # 3 is used to transport the first dilution to the turning valve and
to empty the cup completely after the cycle is finished. Pin 4 is used for air evacuation and acts also
as a prediluted sample inlet. Pin 3 and 4 are used as electrodes for liquid sensing as well.
Washing device
Asp.
needle
Waste
Diluent
inlet
Wash
Device
Fig. 1F
The aspiration needle washing device is connected to the waste outlet ( pump ). The diluent used
for the aspiration needle cleaning is flushed from the top of the needle downwards to the washing
cup from where it is flushed to the waste outlet.
The washing device contains a magnetic sensor used to inhibit the washing cycle if the sample was
not removed from the aspiration position.
-----------------------------------------------------------------------------------------------------------------------------------
1.2 WHOLE BLOOD ASPIRATION
When the whole blood start lever is pressed, first the RBC counting chamber is emptied by opening
valve 15 and 12. Simultaneously, the pistons are moved to their far down position.
This action creates a slight vacuum in the RBC counting chamber and is ‘controlled’ by the
opening duty cycle of valve 12. I.e. , valve 12 is pulsed to get an air bypass trough the waste pump
(P). Valve 2 is now opened and the blood is aspirated through the aspiration pipette at position 1 in
the turning valve.
See figure 2 below.
The measuring chamber of the WBC is emptied by opening valve 18b and 13b after the HGB blank
is read on the remaining ( blank ) diluent.
The blood flow is stopped by a blood detector, which is an optical device sensor, located between
the outlet of position 1 of the turning valve and valve 2b.
The blood detector can be disabled and replaced by a timing device within setup-menu2. This
allows the system to be used for platelet-rich plasma and other none-whole blood applications as
well. In such a case the aspiration is terminated by the custom programmed timing sequence.
Note that if no blood is detected ( if the blood detector is enabled ), a time-out of 10 seconds will
abort the aspiration and the cycle will continue to enable ‘blank’ checking of the CA530.
-----------------------------------------------------------------------------------------------------------------------------------
1.3 FIRST DILUTION
The first dilution is made by positioning the turning valve to its second position (B) . The micro
volume of blood is now on one end connected to the syringe filled with diluent and the other end
to the inlet (2) of the mixing cup via valve 9b , 6a and 8b.; valve 2a is closed.
The syringe has a mechanical fixed volume of ca. 5.2 ml.
During the start of the upwards movement of the pistons, valves 11, 16, 12, 13b, 17 and 14 are
opened. Therefore, the diluent in the metering units is moved to the start detectors, where after the
valves 11, 14 ,16, 17 and 13b are closed again. ( Valve 12 remains open )
The piston is moved upwards to it’s far upper position, preparing the first dilution in the mixing
cup. As no lyser is needed; this is flushed backwards into the lyser container via valve 10a.
Air evacuation of the primary mixing cup is performed by opening valve 7a
When the WBC piston arrives in it’s fixed upper position, the volume below the WBC piston
contains vacuum. This is used to mix the primary dilution. See section 1.4
-----------------------------------------------------------------------------------------------------------------------------------
1.4 MIXING THE FIRST DILUTION
The first dilution in the mixing cup is mixed by first closing the turning valve to a position between
A and B and valve 7a is closed. Vacuum is created in the mixing cup by opening valve 13b as the
measuring chamber of the WBC syringe contains vacuum.
Valve 7a is now opened and air is flushed trough the diluted sample.
See fig 4 below.
-----------------------------------------------------------------------------------------------------------------------------------
1.5 PREPARING THE FINAL DILUTION
After the first dilution is mixed, the turning valve turns back to position A. Valve 18b, 17, 4, 6b
and 8a are opened and valve 7a is closed.
The first dilution will now flow through the tube marked V1-V2 towards the WBC measuring
chamber and finally to the waste outlet.
The volume between point V1 and V2 is fixed at 2.4 ml. The initial volume in the mixing cup was
ca. 5.2 ml. Pin 3 and 4 in the mixing cup are acting as liquid sensors during this ‘sample transfer’
process and valve 4 is closed as soon as no liquid is detected between these 2 electrodes.
Ca. 0.5-1 ml will be left in the mixing cup which means that V1-V2 is fully filled with the first
dilution. ( as : 5.2-0.5 >> 2.4 )
A time window is checking that the ‘transfer’ is done within a specific time limit. A too short time
will indicate that either no liquid is present in the mixing cup or electrodes 3 and 4 are non
conducting or contaminated . A too long time indicates most probably a blockage in pin 3 of the
mixing cup. Proper error numbers are displayed to trace these problems. See section 4.3 Error
Indications for further info.
-----------------------------------------------------------------------------------------------------------------------------------
1.6 CLEANING THE ASPIRATION PIPETTE
The aspiration pipette is cleaned by moving the pistons partly downwards while valve 9a and 10a
open. Ca. 1 ml of diluent is now flushed over the aspiration pipette by opening valve 9b, 5 and 2b.
The waste pump is on, and the pistons are moved back to their upper position . Finally 3 a is
opened to empty the washing device cup. Therefore, the aspiration pipette is cleaned on the inside
as well as the outside. Observe that the pipette cleaning process, also flushes diluent through the
turning valve compartment. This is done to exchange the diluent within the turning valve
compartment slowly, avoiding bacterial grow within the turning valve.
Note that this is only performed if the washing device is in it’s upper position, else the cleaning
process is aborted with a few ‘beeps’ to alert the operator to remove the sample from the aspiration
pipette. The washing cup contains a magnet which activates a position sensor. A beep is heard
whenever the washing cup positioning device is triggered.
-----------------------------------------------------------------------------------------------------------------------------------
1.7 FINAL DILUTION
After the pipette cleaning process as shown above, the pistons are moved to their far down position
in order to prepare the final dilution and transfer to the measuring chambers.
The turning valve is turned 45 degrees clockwise to the second position (B). See fig 7 below.
The pistons are moved upwards to their far upper position with valve 9b, 2a, 16 open on the RBC
side and valve 10b and 17 open for the WBC channel.
The final dilution 1:40.000 (RBC) and 1:400 (WBC) will now be flushed to the measuring
chambers below the piston. Note that the volume displacement below the pistons is higher than the
actual volume flushed to the measuring chambers, hence a vacuum is created simultaneously in the
measuring chambers.
When the pistons arrive in their upper position, valve 12 and 13b are opened, this creates a flow of
air through the sample for mixing purposes as well as neutralizing the vacuum on top of the final
dilution in the measuring chambers.
The mixing cup is emptied simultaneously by opening valve 8a, 6b and 3b with the waste pump
switched on. This is not shown in the figure below for clarity purposes only
-----------------------------------------------------------------------------------------------------------------------------------
1.8 START ANALYZING PROCESS
The pistons are moved down to a reference level controlled by the piston position detector as
described in section 3.8 and 5.3
This creates a pressure of ca. 170 hPascal on top of the counting chambers ( marked P in fig. 8)
Valve 11 and 14 are now opened and the sample is forced through the orifices. The diluent is
passing the start detectors, initializing the measuring process , and ends when the stop detectors
detect liquid. Therefore a positive volume of the sample is measured.
The HGB extinction is measured 10 seconds after the start of the WBC counting process.
The aspiration pipette is dried during the counting process by opening valve 2b with the waste
pump on. ( Not shown in fig 8 for clarity )
Note that the volume between the orifices ( cap. ) and valves 11 and 14 is larger than the volume in
the metering units. Hence, the sample will never arrive in the metering unit itself. This avoids any
contamination of the metering unit..
-----------------------------------------------------------------------------------------------------------------------------------
1.9 CLEANING THE MEASURING CHAMBERS
After the diluent arrives at the stop detectors, the parameter results are displayed as well as printed.
The system is now cleaned. The measuring chambers are emptied by opening valve 15b and 18b.
The pistons are moved to their far down position. Next, clean diluent and lyser is used to wash the
mixing cup and the WBC measuring chamber.
The pistons are driven to the top position and valves9b, 6a, 8b/8a are opened to flush diluent to the
mixing cup. Valves 10b and 17 are open to clean the WBC measuring chamber.
After the pistons arrive in their far upper position, the mixing cup is emptied by opening valve8a,
6b and 3b. The WBC measuring chamber is emptied during the next downwards movement of the
pistons by opening valve 18b and 13b.
-----------------------------------------------------------------------------------------------------------------------------------
1.10 FINAL POSITION
The measuring chambers are finally filled with clean diluent / lyser. The non contaminated lyser in
the WBC chamber is necessary to avoid protein built-up as well as making a HGB blank reading
possible on the next sample.
The pistons are moved from their far down position to the final position which is a bout half-way
between the upper and lower position, leaving ca. 3 ml of blank solution in the measuring
chambers. Finally valves 12 and 13b are opened to release the vacuum in the measuring chambers.
So, the home position of the CA530 leaves the system with liquid in the metering units and
measuring chambers.
-----------------------------------------------------------------------------------------------------------------------------------
2.0 PREDILUTED SAMPLES FLUID DIAGRAM
Samples that are prediluted are aspirated though valve 7a.

The system provides a timing sequence to allow the sample to be drawn directly into the mixing
cup. The ‘electrodes’ 3 and 4 in the mixing cup are used to sense if any diluent is present before
the sample is measured. If no sample is entered, the system will restore itself to it’s home-position
which takes ca one minute.
The prediluted sequence starts with emptying the measuring chambers and moving the pistons to
the far down position analogue to the description in section 1.2 except that no attempt is made to
aspirate whole blood .
Valve 18b, 13b are opened to create a vacuum in the mixing cup, where after valve 7a is opened to
aspirate the prediluted sample. The volume that is aspirated is of none importance as long it is
larger than 5 ml. Therefore, the prediluted sample should have a total volume of 5ml to ca. 8 ml.
As the prediluted calibration range is extended, other dilution ratios up to 1:260 can be handled
without any problem. Just calibrate the predilute tables accordingly within the setup-menu
‘Calibration’.
If a volume of less than 5 ml is aspirated, a sealing error in the mixing cup or any other air leakage
which lowers the vacuum might be the source. A low capacity of the waste pump might be the
source as well. After the sample is aspirated, the system performs an analyzing process as described
in section 1.5 to 1.10 above. Note that if dist. water is aspirated, the system will not recognize this
as a valid sample as no electrical conductivity is present between the ‘electrodes’ 3 and 4.
-----------------------------------------------------------------------------------------------------------------------------------
3 SERVICE MENU’s / SPECIAL PROCEDURES
Within the service menu’s several procedures are found to check and to adjust the CA530 system.
This chapter describes all available service menu’s within the system.
The service menu’s are available at 2 levels. The highest level called service-menu2 is used to
adjust the syringe / piston positions and the turning valve. These positions are individual for each
instrument due to mechanical tolerances. The correct settings are stored in an EEPROM IC-33 on
the CPU board. This means automatically that whenever changing a CPU board, the IC-33 should
be replaced by the original circuit to avoid unnecessary re-calibrations of turning valves and / or
pistons.
3.1 VALVE / WASTE-PUMP CHECK
Enter service-menu 6.1 as shown below :
6.1 PUMP&VALVE TEST / TO SELECT 0

PRESS <START> LEVER TO ACTIVATE
The waste-pump is allocated as number 0 , valves are activated by choosing 2-18. The valves are
sequentially numbered from left to right at each row. Pressing the whole-blood start-lever will
activate each individual valve. Check by hand that the plunger is retracted completely. Note that the
voltage measured over the valve is ‘popping’ up to ca. 28 Volts and falling to ca. 22 Volt after ca.
300 msec. A double valve ( A and B ) has a different coil rating than a single valve. Resistance in a
double-valve coil is ca. 75 ohm, a single valve has ca. 150 ohm.
A mal-function of a valve is usually located in the mechanical part only. If a valve plunger is not
retracted completely, change the plunger only to avoid unnecessary soldering on the valve PC-
boards. Remove the clip from the rear end of the plunger to replace this part. See figure below :
Clip
Position
-----------------------------------------------------------------------------------------------------------------------------------
Fig. 12
3.2 PHOTOMETER CHECK / ADJUSTMENT
The photometer consists of a tungsten lamp / cuvette / filter and a photo-diode. The filter is of a
530 nm wide band type with a pass-band of ca. 20 nm. As the specific wavelength for a none-
cyanide reagent is between 500 and 600 nm, narrow band filters are not used. The filter is of a
glass-type ( not interference type !), giving it an unlimited life-time. Don’t change the filter unless
it is visible cracked. The filter is located on the rear side of the chassis between the cuvette and the
photo-diode. ( within the photo-diode holder )
The voltage over the lamp is controlled by the CPU, the output voltage from the photo-diode is
amplified and shown on the display in service menu 6.2 as below :
6.2 LAMP= 4.70 V PHOTOCELL =3.50 V

PRESS <1> FOR AUTO ADJUST, / TO TEST
The lamp voltage is switched off when the CA530 enters the stand-by mode. On an ‘exit-standby’,
first the offset of the amplifier is measured and stored, thereafter the lamp is switched on to its
nominal value . As the output voltage from the amplifier should be within a certain range to be
correctly read by the A/D converters, a defined ‘blank’ voltage range is accepted by the system.
The photocell voltage, on a blank, may never exceed 4.0 volts and should not be lower than 2.8
volts. In such a case the HGB will be marked with HI or LO indicating that the blank level is not
correct.
A LO indication might be caused by :
a. Contaminated cuvette
b. Faulty lamp
c. No blank ( lyser ) in the cuvette ( = WBC measuring chamber )
To clean the WBC chamber in a fast way, a syringe with hypo-chlorite or ( better ) enzymatic
cleaner is attached as shown below :
-----------------------------------------------------------------------------------------------------------------------------------
WBC Measuring
Chamber
WASTE OUTLET WBC
CLEANER
Fig 13
Wash the chamber by flushing the cleaner in and out several times. An alternative is to fill the
complete system with an enzymatic cleaner ONLY ( don’t use hypo-chlorite unless the system is
contaminated with a bacterial grow !) and perform a ‘FILL-SYSTEM’ from the Flow-Menu.
After this cleaning process, a fill-system should be performed to clean the chamber from any hypo-
chlorite ( if used ) where-after digit 1 is pressed in menu 6.2 to perform an automatic adjustment of
the photometer lamp voltage.
If no automatic adjustment can be performed ( digit 1 in menu 6.2 ) due to a too low light level
from the lamp, Indication # 9 will be displayed. Cancel this by pressing <CE> and replace the
lamp. However, check again that this indication was not caused by a leak of lyser in the measuring
chamber !
After the lamp is replaced, perform again the automatic adjustment ( digit 1 in menu 6.2 ).
The output voltage from the photocell is put at 3.50 volts during the auto-adjustment. If this voltage
is not reached completely, trimpotentiometer RV1 on the analogue amplifier board can be
adjusted.
This potentiometer can be reached without removing the cover of the analogue board. See fig. 14
below.
Photom. Coax
adjust. Photom.
RBC
WBC
Fig 14
Do as follows in menu 6.2

-----------------------------------------------------------------------------------------------------------------------------------
a. Put the lamp-voltage at 4.60 volt with the / keys

b. Turn trimpotentiometer RV1 to read 3.50 volts on the photocell-output
c. Press digit 1 to complete the adjustment.
Normally, a lamp voltage of ca. 4.70 volt will give an output voltage of 3.50 volt.
Check that this voltage is stable. Instability can be caused by airbubbles in the WBC measuring
chamber or a bad electrical contact at the lamp-socket
On each sample the HGB is calculated as :
HGB = F* Log ( Blankvoltage-Offsetvoltage / Samplevoltage-Offsetvoltage)
Where F consists of the calibration factor and the specific HGB molecular absorption factor.
The photometer offset is read on menu 6.2 by removing the coaxial cable connected to the photo-
diode from the analogue amplifier board.
This voltage must be between 0.03 and 0.5 volts and should be completely stable within 0.02 volt.
If this is not the case, the analogue PC board 530-9021 must be replaced.
3.3 START / STOP DETECTOR CHECK / ADJUSTMENT
The start / stop detector at the metering units consists of an infra-red LED and a photo-transistor.
Due to a large difference in refraction index between air and liquid, a significant change in output
voltage from the photo-transistor is detected . In service-menu 6.3 the status of these detectors is
read in real-time. A ‘1’ represents liquid and ‘0’ air.
In case the metering unit ( glass tube ) is replaced, the following must be checked and possibly
adjusted.
In this service-menu, the letter ‘L’ stands for the lower detector ( start ) and ‘U’ the upper detector
( stop ).
The Infra-LED’s in the start / stop detectors are only switched on during sample aspiration and in
service-menu 6.3 , else they are switched off.
6.3 START/STOP DETECTOR STATUS

RBC-L=1 RBC-U=1 WBC-L=1 WBC-U=1
The CPU board has 4 Red-LED’s connected to a window-discriminator, showing the status of the
start / stop detectors. This circuit makes a correct adjustment possible without using any external
voltmeter.
-----------------------------------------------------------------------------------------------------------------------------------
Do as follows to adjust the start and / or stop detector :
a. Enter the service menu 6.3

a. Connect a syringe with dist. water to the lower end of the glass-tube
b. Fill the glass tube to slightly over the stop detector.
c. Locate the LED’s and trimpotentiometers as shown below on the main CPU board .
See Fig 15
R
V LD5 LD6 LD7 LD8
Cable to 1
start / stop
detectors..
R
V
2
R
V LD4 LD3 LD2 LD1
3
R
V
4
Fig 15
Start / Stop Trimpot. LED showing liquid LED showing air
WBC-L RV4 LD2 LD1

WBC-U RV3 LD4 LD3
RBC-L RV2 LD6 LD5
RBC-U RV1 LD8 LD7
To adjust the WBC-L detector ( start detector WBC ), turn trimpot. RV4 so that LD 2 is just
flashing between on and off. Now turn this trimpot. 1 turn towards the ON position.
-----------------------------------------------------------------------------------------------------------------------------------
To adjust the WBC-U detector ( stop detector WBC ), turn trimpot. RV3 so that LD 4 is just
flashing between on and off. Now turn this trimpot. 1 turn towards the ON position.
To adjust the RBC-L detector ( start detector RBC ), turn trimpot. RV2 so that LD 6 is just flashing
between on and off. Now turn this trimpot. 1 turn towards the ON position.
To adjust the RBC-U detector ( stop detector RBC ), turn trimpot. RV1 so that LD 8 is just flashing
between on and off. Now turn this trimpot. 1 turn towards the ON position.
d. Empty the glass tube completely and check that the corresponding ‘Air’ LED’s are lit as shown
in the table above.
Note that this adjusting method is more accurate than just checking the status in service-menu
6.3 as the LED’s 1-8 in the figure above are connected to a window-discriminator instead of a
one level trigger-point only.
3.4 BOTTLE DETECTORS & BLOOD DETECTOR ADJUSTMENT
Service menu 6.4 shows the status of the external Diluent and Lyser bottle as well as the current
status of the internal Mixing cup as well as the blood detector in real-time.
Liquid detection is performed by sending a puls towards one electrode and measuring the return
voltage from a second electrode. The liquid detector sensitivity cannot be changed and is only
activated if the diluent and lyser have enough electrical conductivity. Replacing diluent / lyser with
dist. water will therefore be indicated as ‘reagent low’ by the CA530. The liquid detection circuit is
only activated if the software ‘needs’ to detect liquid and in this service-menu 6.4. Else the
detection circuit is switched off.
6.4 BOTTLE STATUS

DILUENT=1 LYSER=1 CUP=0 BL.DET=1.5 V
A ‘1’ shows reagent present, a ‘0’ means ‘bottle empty’.

The detection circuit in the internal mixing cup can be checked by filling a syringe with diluent
connected to the prediluted inlet.
Checking / adjusting the blood detector
The blood detector consists of a green LED as light source and a photo-transistor as detector. The
photo-transistor is connected to an A/D converter and the output voltage is read on the display. A
voltage over 3.5 volt is seen by the system as being ‘blood’. All voltages below 3.5 are ‘no-blood’.
-----------------------------------------------------------------------------------------------------------------------------------
The total range of this voltage is 0-4.1 Volt

This detector needs to be checked during the planned service intervals. Proceed as follows to check
this detector .
a. Dilute externally a blood sample with diluent to an HGB of ca. 0.5 - 0.8 g /dl
b. Goto the service menu 6.1 and press the whole-blood start-lever ( the waste pump is started)
c. Enter the diluted sample at the whole-blood inlet and press valve 2 by hand. The diluted sample
is now aspirated through the blood detector. Release valve 2
d. Enter the service-menu 6.4 again and observe the blood detector voltage.
e. Turn trimpotentiometer RV5 on the CPU board so that 3.5 V is read

( a beep is heard whenever triggering this 3.5 volt level ).
This potentiometer, on the CPU board, is located just beside the connector of the blood-detector
cable.
3.5 TURNING VALVE & PISTON DETECTOR CHECK
Service menu 6.4 provides a quick check on the turning valve and piston detector status.
The turning valve position is read in real-time from the connected high precision potentiometer.
A typical value when entering this menu should be between 1500 and 2500. See further section 3.7
for detailed explanation how to adjust the turning valve.
The piston detector consists of a LED and photo-transistor indicating the position of the pistons.
A ‘1’ means that light is seen at the photo-transistor. Refer to section 3.8 and 5.3 for detailed
instructions how to adjust the pistons.
6.5 MOTOR DETECTORS

TURNV = 2185 PISTON = 1
3.6 MAINS SUPPLY NOISE TEST
Service menu 6.6 shows a simple noise analyzer which is of good use whenever the CA530 is
installed under primitive conditions with low quality mains-supply or grounding possibilities.
A real-time amplitude and frequency measurement is done from the analogue amplifiers. This
display should always show zero on all parameters. If this is not the case, an external interference
occurs and an extra line filter ( and/or ground system) should be installed.
The amplitude is maximum 2047 which corresponds to 250 fl on RBC and 400 fl on WBC.
-----------------------------------------------------------------------------------------------------------------------------------
E.g. : an indication of RBC AMPL = 100 and FREQ= 100 shows an interference equivalent to an
amplitude of ca. 12 fl and a frequency of 100 Hz which results in a size distribution curve on PLT
with a mode of ca. 12 fl.
The CA530 conforms to EMC 801-1 regulations which corresponds to minimum 500 V amplitude
line interference. ( Typical value for the CA530 is ca. 700 Volt up to 2 GHz )
In case this menu shows any data, the local mains supply should be analyzed and proper action
should be taken to cancel the source of the line-interference.
6.6 AMPL. FREQ. AMPL. FREQ.

RBC=0 0Hz WBC=0 0Hz
-----------------------------------------------------------------------------------------------------------------------------------
3.7 TURNING VALVE ADJUSTMENT
The turning valve is enclosed in an hermetically sealed compartment and surrounded by diluent.
The valve is turned by a motor device and the position is read by a high precision potentiometer
directly connected to the axis. After the A/D conversion, the position is compared by the CPU to
the preprogrammed position as stored in the EEPROM. A calibration program is available from the
service-menu2 to define the exact positions of the turning valve ( Position A and B ).
Therefore, the EEPROM IC-33 contains the data that is specific for each unit. In case a CPU board
is changed, it is advisable to replace the EEPROM on the new board with the EEPROM of the old
PCB.
Note that no adjustments are necessary on the turning valve system during any normal service.
Never dismantle the potentiometer from the axis if not absolutely necessary !
The description below is for reference purposes only and should NOT be carried out during any
form of regular service .
POT.METER
MOTOR
VALVE
A/D
CPU
Fig 16
Needed tools :
a. Calibrator pin with 1.0 mm diameter

b. Plexiglas adapter ( available from Medonic )
(The Plexiglas adapter is only necessary if a larger adjustment has to be made as the micro channels
will be visible.)
Procedure :
1. Remove the probes from the reagent containers and go to the system flow menu to perform an
‘Empty System’
2. Remove the front ‘seat’ of the turning valve and replace it by the Plexiglas adapter. See figure
17 below. Be careful not to scratch the turning valve or front-seat surfaces.
-----------------------------------------------------------------------------------------------------------------------------------
3 screws
Fig 17
3. Enter service-menu2 6.9.1 . The following is displayed:
6.9.1 TURNV. POSITION=2 A/D-VALUE=1673 ----

TEST=/USE <START> LEVERS TO ADJUST
The turning valve is in position 2 ( Pos. B , see fig 18 below ) with the corresponding A/D value
shown .
Note that this value should be between 1300-2000 . In case the potentiometer was de-mounted /
replaced ; note that the potentiometer might be 180 degrees of position. In such a case remove the
potentiometer and rotate the axis by hand to the position as shown below. Than remount the
potentiometer in the correct way.
2 2
Fig 18
Use the calibrator pin in positions 2 and 3 , they should go freely into the turning valve channels.
The start lever whole-blood is used to rotate the turning valve in steps of 0.7 degrees counter-
clockwise The start-lever for prediluted samples is used to rotate the turning valve clockwise in 0.7
degrees steps. Continue until the calibrator pin goes freely into all positions 2 and 3.
4. Press the  key to rotate the turning valve to the home position 1 ( Pos. A, fig 19 ) and repeat
the adjustment the same way for channel 1 and 2. See figure 19 below.
-----------------------------------------------------------------------------------------------------------------------------------
2 2
1A
Fig 19
5. Press MENU only once and enter the code 6307. Confirm with enter.
This stores the new positions in the EEPROM circuit.
6. Remount the front-seat and check the above procedure again.

Note that position 1A of the turning valve cannot be checked with a 1.0 mm calibrator pin
whenever the original front-seat is in place.
7. After finalizing the adjustments, do a ‘ Print Machine statistics’ from the service menu. The A/D
values for the programmed positions are printed for reference purposes.
Note :
Within service menu 6.9.1 the field ‘ A/D-VALUE’ accepts also numerical values. The valve can
be turned directly by typing in a value from the keyboard. However, if the typed value has a too
large difference compared to the current displayed value, it is not accepted by the software.
-----------------------------------------------------------------------------------------------------------------------------------
3.8 PISTON ADJUSTMENT
The adjustment of the piston position is not necessary during the normal planned service intervals.
The procedure below is only for reference purposes.
The pistons are connected to a stepping motor device driving the pistons which have mechanical
stops in their end positions. (Upper and lower). The total stepping range of the motor, between the
upper and lower position, can be programmed to assure that both end positions are reached with the
springs compressed at least 1 mm. The reference level of the total programmed stepping range can
also be set. This means that the total stepping range can be moved either upwards or downwards. A
correct adjusted system will reach the end positions with the springs at least 1 mm compressed.
The reference point is built by a photo-detector and a pin mounted directly on the piston axis
triggering the optical device. It is essential that the distances are kept as shown below. In case a part
is exchanged, FIRST check and adjust the parts according to the following measures.
54.5
4.5
71.5
20.7
Fig 20
-----------------------------------------------------------------------------------------------------------------------------------
Procedure :
1. Enter service-menu 2 , menu 6.9.2

The following is displayed :
6.9.2 PISTON DET_FROM_TOP=10.5 -----

TEST= / RUN_LENGTH=242
----
The arrow keys will move the piston in either the far up or far down end position, check that, in
both end-positions, the springs are at least 1 mm compressed.
2. Change the total stepping range by putting the cursor with the <ENTER> key in the field
‘RUN_LENGTH’ and key in a different value. A large value will increase the stepping range.
3. If at one end position the springs are considerably more compressed than the opposite side,
change the reference point by first putting the cursor in the DET_FROM_TOP field with the
<ENTER> key. A larger figure here will move the total stepping range UPWARDS. A lower figure
will move the range DOWNWARDS.
4. After the adjustment, press <MENU> only once and enter the code 6307. Confirm with
<ENTER> This will store the settings in the EEPROM circuit IC-33
5. Restart the CA530 by removing and reconnecting the power cord and run a blank sample. Check
that the springs are correctly compressed at their end positions. repeat the above if necessary.
-----------------------------------------------------------------------------------------------------------------------------------
3.9 “ PRINT ALL SETTINGS”
“Print All Settings” from the SET-UP MENU is performed after a first time installation of the
CA530 and if any service is needed from Medonic.
All user programmable settings are printed and kept as a reference for later service and/or
adjustments.
Below follows an explanation of some of the printed parameters as most of the values are obvious.
Program Version
As this manual is written for program version 1.88, any additional info on later versions is found in
the Appendix Section (12) of this manual.
Calibration
Here the current calibration values in % are printed for all blood inlets.
The RDW and PDW calibration factors are printed in 4 digit series, representing the percentage of
the curve of which the calculation was made. E.g. 6500 means 65.00%
Parameter normal range settings

The CA530 has 9 normal range settings, which are linked to the used discriminator program shown
as ‘PROG’ during the blood aspiration and on printed sample data.
Floating discriminator settings

The 9 different floating range settings are printed here. They represent the settings of PROG 1 to
PROG 9
Turning Valve Positions

The turning valve has 2 programmable positions as described in section 3.7 of this manual.
The printed positions are slightly individual for each CA530 and are of importance whenever a
CPU is exchanged without changing IC33 ( see section 5.1 ) . Setting the positions back to the
original values as printed; makes a new calibration of the turning valve not necessary.
Syringe positions
The syringe positions are calibrated in Service Menu 2 , see section 3.8
The printed positions are slightly individual for each CA530 and are of importance whenever a
CPU is exchanged without changing IC33 ( see section 5.1 ) . Setting the positions back to the
original values as printed; makes a new calibration of the piston motor not necessary.
‘Det’ is a virtual reference point in mm with the optical piston position detector as the physical
reference. In other words; the offset of the total stepping length in respect to the optical piston
reference point.
‘Len’ is the total stepping length of the piston motor.
-----------------------------------------------------------------------------------------------------------------------------------
Syringe Init
The piston motor backlash values are printed here.
e.g. , a value of 14 0 1 represents :
14 = Backlash in up/down direction in tenths of millimeters. ( = 1.4 mm )

0 = Reproducibility of the backlash in upwards direction in tenths of millimeters ( = 0.0 mm)
1 = Reproducibility of the backlash in downwards direction in tenths of millimeters ( = 0.1 mm)
The backlash should be between 05 and 22 ( 0.5 to 2.2 mm ) and the reproducibility less than 2 ( <
0.2 mm)
HGB lamp voltage

The lamp voltage is printed here. Normal values are from 4.50 to 4.85 volts.
In case a value of > 4.90 is printed; follow the instructions in section 3.2
Auto Print Function

The setting as defined in the Main-Menu point 2
Print Key Function

Ticket Print Format

Default Discriminator Program

The default setting of the discriminator program as from Main-Menu point 8.
( Shown as ‘PROG’ during sample aspiration and on sample printouts )
Language
The language in use. Refer to the User-Manual and/or Appendices regarding available language
settings.
Unit System
The unit system in use. Refer to the User-Manual section 4.8 for available parameter formats.
Machine Id
The settings from Menu 5.9.3 . See User-Manual section 5.14
Blood detector / time

If the CA530 is used for whole blood only, the setting should be 0.0, which enables the blood
detector. In case the system is also used for Platelet Rich Plasma a time setting of 1-3 seconds is
expected. Refer to section 5.14 in the User-Manual.
Serial Port
The settings of the serial output, Menu 5.9.5. Note that the Hardware-Handshaking should always
be ‘Y’
-----------------------------------------------------------------------------------------------------------------------------------
Barcode reader type

Should be ‘0’ in case no Bar-Code reader is installed. Else, refer to section 7.3 of this manual.
PLT offset
Refer to the User-Manual section 5.14 for detailed info.
High Altitude Compensation

The setting of Menu 5.9.8. Refer to section 5.14 in the User-Manual for detailed info.
3.10 “ PRINT MACHINE STATISTICS”
The ‘Print Machine Statistics’ as found in the Service-Menu, gives necessary statistics and settings
to simplify service and maintenance.
Below follows a description of the printout.
Init
The very first time the CA530 was powered-on at the final control stage.
Power on
Last cycle
A ‘Last Cycle’ can be any cycle of the CA530, e.g. Prime, Fill, Count etc.
This printout can be used to check if the CA530 was powered-off for a longer period of time.
e.g.
Power on = 20/05/1998 17:39 ( Power was switched on : 20 May 1998 at 17:39 h)
Last Cycle = 20/05/1998 18:05 ( A cycle was performed at 18:05 h )
Power on = 23/05/1998 18:03
The above example shows that the CA530 was switched off 3 days with an accuracy of maximum -
-4 hours. ( As a washing cycle is performed every 4 hours )
Indication
The type and date-stamp of the Indications.
e.g.
Indication = 203 Cycle=3 13/05/1998 18:55
Indication = 303 Cycle=3 13/05/1998 18:55
The user pressed the start-lever but the internal mixing cup contains diluent ( Indication 203 ). The
start is aborted ( Indication 303 ). See further section 4.3
Calibration
The last calibrations / time-stamps.
Note that if the CA530 is calibrated several times within 1 hour; only the last is logged.
Blood Samples
The total number of blood samples counted from the Init date.
A blood sample is defined as a sample with a minimum RBC of 1.00 or PLT > 100
-----------------------------------------------------------------------------------------------------------------------------------
Blank Samples
The total number of blanks. A ‘Blank’ is defined as a ‘Sample’ with RBC < 1.00 and PLT < 100
Prime Cycles
Total number of ‘Prime Cycles’ initiated by the user.
Wash Cycles
The total number of “4 hour” wash cycles. Note that if the CA530 is never used, but powered-on;
there will be 6 wash cycles / day.
Overhead
The % of the reagent consumption used in ‘Blanks’. (performed by the user)
Total overhead
The % of the total reagent consumption used in ‘Primes’ , ‘Wash Cycles’ and ‘Blanks’
In other words; all reagent consumption that is not related to a ‘Sample’
Note: If the CA530 is effectively used for 50 to 150 samples / day. This figure should be between 5
to 15 %
Orifice cleans
The total number of manual and automatic orifice clean cycles.
Man : Number of manual orifice-cleans from Menu 6.8
Ar : Number of automatic orifice cleans, RBC channel
Aw: Number of automatic orifice cleans, WBC channel
Last sample Statistics.

The following is related to the last sample only.
Mode
See user manual section 8.4 ( Serial Output Format ) at ‘MODE’
RBC count time

WBC count time
The counting time in milliseconds of the RBC and WBC channel
Aspiration time
The first printed value is the time, in milliseconds, for the blood to reach the blood detector at the
whole-blood inlet. e.g. 1423 means 1.423 seconds.
‘Value’ , shows the value from the A/D converter connected to the photocell of the blood detector.
As blood has a high light absorption with HGB values of > 3 g/dl, the value is always 4095, which
is the maximum level. A ‘Blank’ will have an aspiration time of c. 10000 ( 10 seconds ) and a
‘Value’ of < 3200 . See further section 3.4 .
-----------------------------------------------------------------------------------------------------------------------------------
Transfer time
This is the transfer time as described in section 1.5 . It is important that the meaning of this ‘Time’
is fully understood. On whole blood samples, this value is normally between 2400 – 4500
milliseconds. The maximum time is 10000 ( 10 seconds ) after which Indication 202 is displayed.
Refer to section 4.3 in this manual.
A high ‘Transfer Time’ and a high ‘Aspiration time’ usually indicates a mal function of the waste
pump membrane(s).
HGB dark
This is the voltage ( in millivolt ) read from the photometer with the lamp switched off. The value
should be between 50 to 300 normally. In case the voltage is 0 or > 500, an electronical failure or
condensation on the analogue PCB is expected.
The value printed between parenthesis (..) is the Sd ( Standard Deviation ) of 30 readings of the
dark-voltage. A ‘0’ means that the voltage is perfectly stable. A value lower than ‘10’ is normal.
A value higher than ‘20’ will print an ‘SE’ flag at the HGB parameter results. A high Sd on the
dark- voltage is usually related to an external interference source . ( Noise from the mains supply)
HGB blank
This is the voltage ( in millivolt ) read from the photometer reading the ‘Blank’ on each sample.
The value should be between 3300 to 3800 normally. In case the voltage is outside this range; the
HGB is flagged with ‘LO’ or ‘Hi’ . Refer to section 3.2 in this manual.
blank-voltage. A ‘0’ means that the voltage is perfectly stable. A value lower than ‘10’ is normal.
A value higher than ‘20’ will print an ‘SE’ flag at the HGB parameter results.
HGB sample
This is the voltage ( in millivolt ) read from the photometer reading the ‘Sample’.
sample-voltage. A ‘0’ means that the voltage is perfectly stable. A value lower than ‘10’ is normal.
A value higher than ‘20’ will print an ‘SE’ flag at the HGB parameter results.
HGB init
The values are obtained from an initial self-test at a power-on and monitors instability possibly
caused by condensation.
SELF TEST a min OFFS.

bbbb cccc dddd mV
-----------------------------------------------------------------------------------------------------------------------------------
At power-on, the CA530 enters the above mode. The photometer offset is measured immediately
and displayed in field (dddd). Each minute the offset voltage is measured and compared with the
previous minute. If no noticeable ( < 10mV ) drift is observed; the program leaves this mode and
continues with the power-on sequence. As long as the drift is > 10mV / minute, the CA530 will
stay in this test-mode and start the waste pump to create a temperature raise inside the CA530. At
(a) the total time before the offset became stable is displayed. If no stable offset is seen after 15
minutes, the analyzer will continue it’s power-on sequence anyhow.
Display fields :
(a) Minutes of test ( max 15 )
(bbbb) Last measured offset
(cccc) Previous measured offset
(dddd) First measured offset
e.g. HGB init = 2 135 138 138 means that the test took 2 minutes ( = minimum time ) and that
the maximum drift of the offset was 3 millivolts.
Rates
The CA530 performs a ‘Counting Rate’ analysis during the counting procedure and calculates the
Sd ( Standard Deviation ) of a group of values. The printed values are the ‘Counting Rate’ mean ,
min and max. ( The Sd itself is not printed )
C. 20-30% difference between the min. and the max. values is normal.
Higher differences might indicate a leakage in the measuring chamber(s) during the sample
analysis. In such case, an ‘SE’ flag might be displayed at the RBC and/or WBC and/or PLT
parameter(s) if it was not a temporary clogging.
Note : ‘Print Machine Statistics’ cannot be reset by the User. A hidden code to reset the statistics is
available from Medonic. In such case the ‘Init’ date will be set to the date of the ‘reset’ and all
other statistics to ‘0’. This is only done in very exceptional cases !
Request bulletin 530-31-203 via e-mail or fax for information.
-----------------------------------------------------------------------------------------------------------------------------------
4 INDICATION NUMBER ### DISPLAYED
Indication numbers in the CA530 are displayed for specific error situations. Most of these errors
are recoverable by restarting the instrument and pressing the <CE> key. Indication numbers are
divided in groups in order to simplify error tracing. An indication number will automatically force
the instrument into a save position. All motors are stopped and all valves are released.
4.1 INDICATION 1-99
INDICATION 1
The date and /or time is not set. Set the date and time in the set-up menu .
INDICATION 2
The safety cover ( start plate ) of the Cap Piercing Device was turned back after a ‘start’ before it
was locked by the motor device.
This is a recoverable Indication. The CA530 does not need to be restarted.
INDICATION 9
Automatic adjustment of the blank HGB voltage failed. Refer to section 3.2
4.2 INDICATION 100-199
Note : An Indication 111-114 might be displayed if the photometer was wrongly adjusted. In such
case, remove the coaxial cable of the photo-diode from the analogue amplifier board . Reconnect
the instrument to the mains-supply and perform a PRIME. Go to service menu 6.2 and reconnect
the coax-cable. Set the Lamp-voltage to 4.6 Volt with the / keys and adjust the potentiometer
as shown in Fig 14 to 3.5 Volt Photocell output. Perform an automatic adjustment by pressing digit
‘1’. The system will now be restored automatically. See further section 3.2
INDICATION 111
The turning valve could not be initiated. Motor failure or driver failure expected Check also the
connection cable. Press <CE> and refer to section 3.7
INDICATION 112
Failure during the start of a turning valve action. A wrong value is read from the potentiometer and
/ or A/D converter. Press <CE> and refer to section 3.7
-----------------------------------------------------------------------------------------------------------------------------------
INDICATION 113
Failure during the end of a turning valve positioning. A wrong value is read from the potentiometer
and / or A/D converter. Press <CE> and refer to section 3.7
INDICATION 114
No initialize of the turning valve possible. Probably due to a faulty motor or loose connector.
Press <CE> and refer to section 3.7 to test the turning valve.
INDICATION 121
No initialize of the syringe possible. Probably due to a faulty motor or loose connector.
Press <CE> and refer to section 3.8 to test the piston driver.
INDICATION 122
The reference photo detector of the piston failed during the start of the syringe motor Check the
cable connection of the reference photo-detector on top of the piston ( RBC )
Check also the syringe motor cable / connections.
INDICATION 123
The reference photo detector of the piston failed during the end of the syringe motor action. Check
the cable connection of the reference photo-detector on top of the piston ( RBC )
Check also the syringe motor cable / connections.
INDICATION 124
The syringe motor could not be initiated, probably due to a motor failure or motor-driver. Check
connectors of the syringe motor. Cancel this error with <CE> and goto the service menu2 point
6.9.2 . See also section 3.8
INDICATION 131
Initializing error of the Cap Piercing Device motor.

a. Motor error
b. End-switch M1 and/or M2 faulty
INDICATION 132
-----------------------------------------------------------------------------------------------------------------------------------
Signature error, microswitches Cap Piercing Device.

This is only possible in case the M1 and/or M2 micro-switch is wrong connected or faulty.
INDICATION 133
The upper or lower end position of the Cap Piercing Device could not be reached within the
specified time-window.
This can be caused by:
a. Motor is fully or partly blocked during movement
b. M1 or M2 is wrong adjusted ( refer to section 7.4 )
INDICATION 134
This error is possible if an Indication 133 was displayed and an attempt is done to start the Cap
Piercing Device again.
See Indication 133 for error sources.
INDICATION 201
The transfer time of the sample from the mixing cup towards the WBC measuring chamber is too
short. This can be caused by a too low sample volume in the mixing cup or by contaminated
electrodes ( 3 and 4 ). Refer to section 1.5 and 5.6 for a full description.
Press <CE> and perform a PRIME from the system flow menu to restore the system.
INDICATION 202
The transfer time of the sample from the mixing cup towards the WBC measuring chamber is too
long. This can be caused by :
a. Too low capacity of the waste pump.

b. A too large sample volume in the prediluted mode.
c. Shorting between the electrodes 3 and 4 in the mixing cup
d. Blockage in the center electrode (3). Rinse with a needle. ( see section 5.6 )
Refer to section 1.5 and 5.6 for a description of the sample transfer process.
INDICATION 203
Liquid is detected in the mixing cup during a start of cycle. The cause might be :
-----------------------------------------------------------------------------------------------------------------------------------
a. Mains power failure within a cycle .

b. Too low capacity of the waste pump
c. Blockage in the center electrode (3). ( See section 5.6 )
INDICATION 204
No liquid detected in the mixing cup during the start of the sample transfer.
The cause might be :
a. Electrodes 3 and 4 are contaminated. ( see section 5.6 )

b. An attempt is made to run the system on dist.water
c. Wire connection to electrodes faulty
The indication range 300-399 is reserved for power failures indication purposes.
The indication 300-399 occurs whenever the power is disconnected from the CA530 during one of
the following cycles. This indication is in most cases completely recoverable by pressing the <CE>
key and performing a PRIME cycle from the System-Flow-Menu.
INDICATION 301
Initialize cycle aborted .

Press the <CE> key and perform a PRIME cycle from the System-Flow-Menu.
INDICATION 302
Prime cycle aborted .

INDICATION 303
Count cycle aborted .

INDICATION 304
Clean cycle aborted .

INDICATION 305
-----------------------------------------------------------------------------------------------------------------------------------
Predilute cycle aborted .

INDICATION 306
Fill cycle aborted .

INDICATION 307
Empty system cycle aborted .

INDICATION 308
Bottles empty during stand-by mode, valve-only cycle aborted .

INDICATION 309
Exit stand-by cycle aborted .

INDICATION 310
Clean cycle during count aborted .

INDICATION 311
Cap Piercing Device cycle aborted due to a Power-Off or a previous Indication 13x
The indication range 900-999 is reserved for memory check purposes.
INDICATION 901
Configuration in both the RAM and EEPROM are lost. This situation occurs on an non-initialized
PC-Board where no data ever has been written to these devices.
-----------------------------------------------------------------------------------------------------------------------------------
The system needs to be recalibrated on the turning valves, pistons and photometer as a minimum to
make parameter calibration possible. ( see section 3.2, 3.7 and 3.8 )
INDICATION 902
Configuration in RAM is lost. This might be caused by a bad battery or by an exchange of the
RAM circuits on the main CPU board. The sample memory is lost. All other calibrations and
settings are intact as they are stored in the EEPROM.
INDICATION 903
Configuration in EEPROM is lost.

This situation occurs on an non-initialized EEPROM where no data has ever been written to this
device.
The system needs to be recalibrated on the turning valves, pistons and photometer as a minimum to
make parameter calibration possible. ( see section 3.2, 3.7 and 3.8 )
INDICATION 904
An error in the data verification of the EEPROM occurred. This points to a defect EEPROM device
( IC-33) on the CPU board or any circuit connected to it.
INDICATION 905
Both the RAM and EEPROM have correct checksums on their data, but are not equal. This happens
if an EEPROM from one board is inserted in another working PCB. This ‘error’ is recoverable
since the EEPROM data is copied to the RAM circuit. I.e. it is showed only once.
INDICATION 911
Configuration of the Machine status is lost ( see print ‘ Machine statistics’ in the service-menu ) .
This indication occurs if a new ( none initialized ) RAM is inserted or if the battery has been
disconnected or in case of a program (EPROM) update ( See also doc 530-31-201) . The error
indication is shown only once.
INDICATION 921
The stored sample data is lost in the RAM circuit. Might be caused by a faulty battery or RAM
circuit. This indication is also shown initially in some of the EPROM updates ( See also doc 530-
31-201)
-----------------------------------------------------------------------------------------------------------------------------------
All errors in this range are reserved for software engineering purposes only. In case any of these
error occurs during normal operation; change either the EPROM ( Program chips ) and / or the
RAM circuits and report the error number immediately to Medonic / Sweden.
Indication-range 2000-2999 is reserved for CPU errors only. An error in this range is only possible
(sporadic) if a CPU board is initialized for the very first time. I.e. there is no RAM or EEPROM
data available.
In such a case, skip the error by pressing <CE>.
Else, if one of these errors occurs frequently ( except indication 2255 ) , change the CPU board.
The list below is for reference purposes only
INDICATION 2000
Integer division by zero error.
INDICATION 2001
Single step trap
INDICATION 2002
Non - maskable interrupt
INDICATION 2003
Beakpoint trap
INDICATION 2004
Overflow trap
INDICATION 2005
Bounds trap
INDICATION 2006
Unused op-code trap
INDICATION 2007
Escape op-code trap
INDICATION 2031
Illegal interrupt
INDICATION 2033
INT-20H, 2FH error
INDICATION 2095
Math-error
-----------------------------------------------------------------------------------------------------------------------------------
INDICATION 2096
Fatal run-time error
INDICATION 2097
Exit with message
INDICATION 2098
Main-returned
INDICATION 2255
Virtual watchdog time-out
Cause :
a. An extreme concentration of cells in either the RBC or WBC channel.
b. An excessive external interference source is present.

( refer to section 3.6 to test)
c. Heavy noise level due to bacterial grow.
d. Orifice failure ( see section 5.5 to replace )
e. Heavy leakage of the RBC piston
4.7 CPU / SYSTEM BOOTING ERRORS ( BEEP’s)
If the CPU cannot be started due to a mal function within a connected device or due to any internal
CPU failure; the system might respond by a beep sequence making error tracing possible.
During a power-on, please note the following :
1 beep
Normal CPU start, no internal errors found
4 beeps, pause, 4 beeps, ...

RAM circuit error
a. Change the RAM circuits
6 beeps, pause, 6 beeps, ...

Display error
a. Check that display cable is connected
b. Change the display PCB
5 or 8 Beeps, Pause, 5 or 8 Beeps,….

Failure in IC 18 ( ‘GAL’ signal processing circuit)
Stuck Key
-----------------------------------------------------------------------------------------------------------------------------------
If a key is stuck, this is shown in clear text on the display whenever the instrument is powered-on.
5 CHANGING/INSTALLING PARTS , PC-BOARDS etc.
Changing components in the CA530 is in most cases obvious and doesn't need additional
information. This section gives hints on critical parts so that any service can be done without
introducing secondary errors in the system. Special attention should be given to the section
CHANGING PC BOARDS and any service around the turning valve system.
5.1 CHANGING PC-BOARDS
The only boards that need special attention are the POWER SUPPLY board and the CPU board.
To change the power supply board in the CA530, please note the following :
Be VERY careful to connect the transformer wires correctly. Note down the colors to be absolutely
sure BEFORE the old card is removed.
Note down the colors and positions of the 2 power stabilizers mounted on the rear side BEFORE
they are unsoldered. Refer to section 9.2
No additional trimming is further necessary
To change the CPU board in the CA530, please note the following :
To replace the CPU board, first ( and only ) the power supply board must be removed . It is not
necessary to unsolder any connection on the power-supply board or to disconnect the transformer
wiring.
1. Remove the EEPROM memory chip IC-33 from the 'old' board and put this circuit in the same
position in the 'new' board.
By doing this, all turning valve calibrations are most probably not necessary. Check the turning
valve positions anyhow. Refer to section 3.7
If this circuit is not exchanged, you MUST re-calibrate the turning valves as described in section
3.7
2. Perform the blood detector adjustment as described in section 3.4

This must always be done, regardless of exchanging IC-33 on the CPU board.
3. Perform the start / stop detector adjustment as described in section 3.3

4. Perform the photometer adjustment as described in section 3.2

Check that the board jumpers are set correctly. J1 and J2 should be closed.
No other adjustments are necessary. The calibration of the measured parameters will remain as they
are stored in the (old) EEPROM circuit.
-----------------------------------------------------------------------------------------------------------------------------------
5.2 CHANGING PARTS / TURNING VALVES
Parts within the turning valve system are critical. This is the most sensitive part of the CA530 and
all parts within the turning valve compartment should be handled with care. For the internal lay-
out, please refer to the drawing below, 530-03-0xx ( Fig 20A ) ( extract from spare-part drawing
530-4043 )
The surfaces of part number 12 and 10 are critical. Avoid scratching or any other damages on the
surfaces of these parts.
Part number 4 (housing) can be removed by just pulling it forwards, it is NOT fitted to the chassis
but just guided over the 3 pins.
When the turning valve is re-assembled, inspect that the surfaces of parts 12 and 10 are clean and
not damaged. Refer to section 3.7 for the position check and adjustments.
14 4
15 5
7 6
9
8
10
530-03-0xx
12
11
Fig 20A
-----------------------------------------------------------------------------------------------------------------------------------
5.3 CHANGING THE SYRINGE/PISTON
The syringes do not need regular service. However in case the piston ( position number 530-2-124
in Drawing 530-4100 ) has to be changed or tightened, observe the following :
1. Perform an ‘Empty system’ from the System-Flow-Menu
2. Remove the screws marked (A) in the drawing below ( fig 21 )
3. Loosen the screws marked (C)
4. Remove the opto-detector with screws (D) and pull it upwards to be free from the piston
position pin.
5. Pull the cylinder with piston and diluent / lyser inlet firmly forwards and upwards.
The cylinder itself can now be pulled from the O-rings
5. Unscrew the piston with screw (B) and replace.
6. Tighten the screw (B) firmly. The piston must be absolutely tight in respect to the cylinder
wall. Any leakage on the RBC side will cause dropping MCV values.
7. Check that the wires from the orifice are not damaged or loosen.
-----------------------------------------------------------------------------------------------------------------------------------
Cylinder
Piston
B
C
Fig 21
-----------------------------------------------------------------------------------------------------------------------------------
5.4 CHANGING VALVES & TUBES
The valves used in the CA530 are silicon-tube pinch valves. Two different types are used, 1 way
normally closed and 2 way valves. The tube used in these valves has narrow tolerances ( 0.1mm)
and has 1.5mm inside and 3.0 outside diameters. These silicon tubes must always be original from
Medonic, no other suppliers are allowed, to assure trouble free operation of the system.
Note that the normally closed section of the valve will clamp the silicon tube in such a way that if
the CA530 is removed from the mains-power supply during a longer period of time ( > 4 days); it
might stick and has to be opened by hand BEFORE the system is powered on again. See section
‘Installation’ in the users-manual.
The valves are divided into a mechanical and an electrical part. It can be separated into 2 parts by
removing the clip ( see drawing below ).
After the 2 screws are removed, the mechanical part can than be moved forward .
Care must be taken that the valves are absolutely free from salt crystal traces as they can effect the
correct operation of the plunger.
Also note that, whenever changing/checking a tube, the tube must be put back perfectly in place.
The coil has a different power rating for a 2-way and a single valve
Coil Clip Position
Screws
Fig 22
-----------------------------------------------------------------------------------------------------------------------------------
5.5 CHANGING THE ORIFICE TRANSDUCER
The orifice-holder ( fig 23 left) is fitted with two screws to the measuring chamber. The orifices
are identical for RBC and WBC and can be interchanged.
The electrodes, marked A , are of platinum and fitted into the holder with 2 o-rings. Be careful ,
whenever removing a tube from the electrodes, not to change the position of the platinum tubes or
to pull them out of the holder.
The electrodes are interconnected with a platinum wire (B) and connected to the ‘cold’ side of the
coaxial cable. ( shield ). The 0-ring seals the holder within the measuring chamber.
The measuring chamber ( fig 23 right ) has 2 platinum electrodes interconnected with a platinum
wire. The electrodes (C) are connected (D) to the inner wire of the coaxial cable ( hot-side )
Be careful , whenever removing a tube from the electrodes, not the change the position of the
platinum tubes or to pull them out of the holder.
B Orifice
D
C
Fig 23
-----------------------------------------------------------------------------------------------------------------------------------
5.6 CHANGING / CLEANING THE MIXING CUP
Whenever the electrodes of the internal mixing cup have to be cleaned, please note the following :
1. The electrodes are sealed with small O-rings in the inside of the cover.
2. Clean the lower (outside) end of the electrodes (3-4) only as they have a Teflon coating , seen as
a green color.
3. Clean the inside of the electrodes with a needle of 0.8 - 1.0 mm
4. Observe that after cleaning / changing; the electrodes must be put in their correct position by
adjusting them to the following measures :
1 2 3 4
2 Screws
Electrode
Adjustment O-Ring
Screws
O-Rings
33 mm
Center electrode
at bottom level Mix Cup
( side-view )
Fig 23a
5.7 CHANGING THE VIEWING ANGLE (LCD DISPLAY)
The LCD display has a certain range on the viewing angle. This angle can be adjusted in the
following way :
On the PCB, where the LCD is mounted, a trimpotentiometer is found which is reachable without
removing the cover-shield. Use the setting of this trimpotm. to adjust the viewing angle.
-----------------------------------------------------------------------------------------------------------------------------------
5.7 CHANGE OF PISTON ( ROD ) SEALINGS
Please note:
When exchanging the piston rod sealing, follow these instructions carefully to avoid damages and /
or wrong adjustments. Do not remove springs or unscrew the locking rings for those . The sealing
must be exchanged from the lower end of the piston as the piston rod may have scratches from the
screws in the locking rings which might damage the sealing.
If the instructions below are followed, it is not necessary to make any readjustment after changing
the sealing. The position number of the sealing package is : 530-02-123 in Drawing 530-4100
Disassembly: See figure 1-3 in drawing 530-0021 below
1. Unsolder the two wires marked A at the soldering support. Make a note where the wires
were connected.
2. Unscrew the screws marked B including the ground connector ( at the locking screw )
3. Loosen the screws marked C ( not necessary to remove )
4. Remove the whole syringe unit from the chassie.
5. Pull the syringe unit apart ( lower measuring chamber D from glass tube E and glass tube
from upper casing F ).
6. Unscrew the piston top support H.
7. Pull the piston rod upwards through the casing F.
8. Unscrew the screws marked I.
9. Remove the sealing unit marked K
Assembly . See figure 4 in drawing 530-0021 below
1. Keep the metal plate S in position over the piston rod. Pass the complete sealing unit
marked V in the drawing over the rod. Be careful during this operation not to harm the
teflon rings inside ’V’ and check that the sealing unit is located with its u-shaped rubber
profile sealing first entering the rod as in the drawing.
2. Now carefully push the piston rod through the casing F and push the sealing unit into its
correct position ( use some drops of water to reduce the friction between the outher O-ring
of ’V’ and the casing F ). Fasten the screws marked I.
Fasten the piston top support H and fit , if necessary, a new piston top to the piston top
support.
3 Push back the glass tube E over the piston to its position in the casing F .
4 Use a little water to reduce friction between the piston and the glass tube.
Note! If the glass tube has one end grinded ( outside ) this end shall be located upwards into the
casing F
-----------------------------------------------------------------------------------------------------------------------------------
5 If necessary, adjust screw G through the open end of the glas-tube according the section 5.3
in the (this) service manual.
6 Push the measuring chamber back over the glass tube and put the whole unit back into the
instrument.
7 Fasten the screws marked B and C and re-solder the wires at the soldering support A. Check
that the cables are put back in their right positions.
-----------------------------------------------------------------------------------------------------------------------------------
-----------------------------------------------------------------------------------------------------------------------------------
6 TUBING SYSTEM LAY-OUT
The attached drawing shows the physical tube lay-out in the CA530.
The type and connectors are shown with legends. See section 6.1
Never cut tube lengths which might seem to be unnecessary long , they might be restrictors or
volumetric devices.
Drawing 530-3018 CA530 without auxiliary devices installed

Drawing 530-3034 CA530 with AD260 adapter
Drawing 220-3006 CA530 with Cap Piercing Device CT220
6.1 LEGENDS
Tube legends :
S1 Silicone  1.5/3.0 mm
T1 Tygon  1/16" / 1/8" (inch) (  1.6/3.2mm)

T2 Tygon  0.8/2.4 mm
P1 PVC  3/5 mm
PT1 PTFE (Teflon)  1.2/2.0 mm

Connector legends
T220 T connector 2.5mm Insidediam.

L20 L connector 2.5mm Insidediam.
Y220 Y connector 2.5mm Insidediam.
AD Straight connector 1.5mm / 2.5mm insidediam.
DD Straight connector 2.5mm Insidediam.
DC Straight connector 2.5mm / 3.0mm insidediam.
-----------------------------------------------------------------------------------------------------------------------------------
7 AUXILIARY DEVICES
This chapter will provide you with additional information concerning additional external or internal
devices connected to the CA530
7.1 INSTALLING THE PRINTER AND SERIAL OUTPUT
Printer configurations
As long as the connected printer is of the DPU411-2 or DPU414 type, no additional settings are
necessary. Just select the DPU411 settings in the 'PRINT FORMAT´menu .
An IBM compatible printer should be setup in a correct way.

The IBM comp. printer MUST have a speed that allows the printer to print a complete sample
WITH size distribution curves within 70 seconds. Violating this, will result in printer-output error
messages during the normal operating of the CA530.
Proceed as follows :
1. Check which paper format is used ( A4 , 11 or 12 inch are possible)

2. Set the dip-switch ( or software settings) in the printer to this format.
3. Select the PRINT FORMAT menu as found in the main-menu and select the corresponding
format.
4. Set the printer protocol ( by dip-switches or software setting) to IBM format only. ( not EPSON
or HP etc..)
In other words, the dip-switch / software and protocol / paper format setting in the printer should be
the same as the setting in the CA530.
In case an IBM compatible printer is chosen (without using tickets) , please note :
The CA530 software ‘remembers’ the selected format during printing and always tries to use the
paper-format to its maximum. This means that whenever some samples are printed with and other
without curves, the software makes its own decision from which point to insert a form-feed. As a
result of this, the paper-form must be synchronized to the software.
To do this, press <MENU> until the main menu heading is displayed. Press <PRINT> . The printer
and print buffer of the CA530 are now reset and synchronized.
Configuring the serial output
The serial output format/setup is described in the user manual. If any computer / network is
connected to the CA530; the following should be observed.
-----------------------------------------------------------------------------------------------------------------------------------
In the SETUP MENU a menu is found INSTRUMENT-CODE. This is used to give the CA530 an
unique identifier in case several CA530´s are connected to the computer network. ( called the
instrument suffix) e.g. CA530-0 or CA530-1 etc..
The instrument suffix is only transmitted on the serial output, please refer to the user manual for
additional information. concerning the data-format
Before the receiving software on the host computer is developed; it is wise to dump a few samples
to a connected computer as a file to get familiar with the serial format even in case patient and/or
instrument abnormalities are included in the transmitted data.
For latest info on the Windows95 software for the CA530, please connect to our Web-server at
address :
http://www.medonic.se
7.2 SYSTEM STATUS LOGGING
Mainly for production and final control purposes, the CA530 provides a system logging on the
serial output. If a PC ( or terminal ) is connected to the serial output, this option can be enabled by
scrolling to the line :
‘SERVICE MENU’ and typing 6307 and <ENTER> . Typing 7036 at this line will disable the
logging function.
During a count cycle the serial output will now show internal functions of the analyzer during the
count process. The logging sequence is not specified by Medonic and might change without notice.
For the field engineer, this option might be useful as it reports HGB , MCV and count statistics.
7.3 BAR_CODE SCANNER
CA530 serial numbers starting at 2026 are equipt with a CPU Board 530-9001 lay-out version
530-700-2 which supports different type of bar-code scanners. This PCB is backwards compatible
with serial-numbers < 2026.
To install the recommended scanner (ordered from Medonic) proceed as follows :
1. Check that jumpers J2, J3 and J4 are closed on PCB 530-9001 (version 530-700-2)
2. Goto the Setup Menu2 and select Bar-Code Reader = 2
The scanner is now operational. No other settings are necessary. Please find the lay-out of the bar-
code scanner attached to this page ( ZE-84RMD4)
7.4 CAP PIERCING DEVICE CT220

-----------------------------------------------------------------------------------------------------------------------------------
The user handling of the optional cap piercing device model-220 is described in the User Manual
section 6.8 as well as the exchange/service of the needle.
This chapter 7.4 describes the adjustment of micro-switches as well as the built-in blood direction
switch. The CT220 is automatically recognized at power-on by the software, if connected to
connector P 50
7.4.1 ADJUSTMENT MICRO_SWITCHES
Four micro switches are used.
Pos. PC-Board Description

(530/220-9071)
M1 BOT Lowest position of the needle / Open tube inlet blood-switch position
M2 TOP Penetration position of needle / Closed tube inlet blood switch potion
M3 WHEEL Tube Adapter Wheel ( Safety switch )
M4 VSTART Start switch coupled to Start/Protection-Lever
In case the unit has been fully dismantled, the initial positions of the M1 and M2 micro switches
must be located first, within 1 mm. Follow the procedure below :
To adjust M2 , proceed as follows and refer to fig 23c :
1. Turn the worm-wheel by hand in upwards direction so that the distance between the needle
support plate, upper surface, and the ( black colored ) cleaning chamber is 2 mm (-0 / + 0.5 mm )
2. Adjust the support plate of M2 in such way that the micro-switch is just activated.
-----------------------------------------------------------------------------------------------------------------------------------
To adjust M1 , proceed as follows and refer to fig 23d :
1. Turn the worm-wheel by hand in downwards direction so that the distance between the needle
support plate, upper surface, and the ( black colored ) cleaning chamber is 40 mm(-/+ 0.5 mm )
2. Adjust the support plate of M1 in such way that the micro-switch is just activated.
-----------------------------------------------------------------------------------------------------------------------------------
During the needle penetration as well as the retraction of the needle, the time to reach the M2 and
M1 positions is checked by the software of the CA530. In case this time-window is violated, an
Indication in the range of 130-139 is displayed.
So, this fixed software time-window is related to the distance between the micro-switches M1 and
M2. In other words, if these switches are at a wrong position, every time the adapter is started, an
Indication 130-139 will be displayed.
The margin of the software time-window in respect to the distance between the switches can be
checked by printing “ PRINT ALL SETTINGS” from the Setup-Menu . (Note that the last run
sample must be from the Cap Piercing Device.)
On the printed list the following is printed :
Vacutainer ( Cap Pierce Device) = xxxx (4500 5100) 50Hz
The numbers 4500 - 5100 is the fixed time-window. The actual value is xxxx . It is obvious that
xxxx should not be too close to 4500 or 5100. A suitable value for 50 Hz operation is 4650 to 4950.
If a value outside these limits is found, adjust the position of M1 ca. 1mm up or downwards.
-----------------------------------------------------------------------------------------------------------------------------------
If the cap pierce device is used for 60Hz operation ( Jumper on P52 pin 7-8) , the corresponding
time window is :
Vacutainer ( Cap Pierce Device) = xxxx (3214 3643) 60Hz
Checking the M1 / M2 switches
The needle and blood-switch can be put in the programmed positions by entering Menu 6.9.3
6.9.3 CLOSED TUBE TEST, POS. = 1

<1> <2> <3> MOVES, <MENU> CANCEL
Press ‘1’ to move the needle to its down (retracted) position

Press ‘2’ to move to the middle position ( can only be reached from pos. 3 )
Press ‘3’ to move the needle to its upper position.
7.4.2 ADJUSTING THE BLOOD SWITCH
The blood switch is automatically switching the blood flow towards the turning valve system. If
blood is taken from the open tube inlet, the motor will turn the needle to its far down position. The
‘Ring 2’ is pressed by the needle support plate downwards and the switch will be in ‘Pos 2’ .
This position is connected to the whole blood open-tube inlet.
Refer to fig23e
In case the blood is taken from the cap piercing device, the needle will move to its far up position
and switch the blood switch to ‘Pos1’ with ‘Ring 1’
When the needle is now retracted after the blood aspiration, it will move to a middle position (2)
where ‘Ring 2’ is not touched. In other words, the blood switch will stay in the cap piercing device
position until the next sample is an ‘open-tube’ sample.
-----------------------------------------------------------------------------------------------------------------------------------
To check and/or adjust the blood switch position, first M1 and M2 should be checked and/or
adjusted. See section 7.4.1. above.
1. Go to menu 6.9.3 . The following is displayed:
6.9.3 CLOSED TUBE TEST, POS. = 1

<1> <2> <3> MOVES, <MENU> CANCEL
2. Press ‘1’ . The blood switch will switch to ‘Pos 2’ ( open tube position )
3. Put a needle ( or wire ) of 0.7 mm in ‘Pos 2’ and check that the switch and outlet at position 2
are in line. If not , adjust the position of ‘Ring 2’.
4 4. Check point (3) above again by moving the needle up and down using Menu 6.9.3
5 5. Press ‘3’ . The blood switch will switch to ‘Pos 1’ ( cap piercing device position )
6. Put a needle ( or wire ) of 0.7 mm in ‘Pos 1’ and check that the switch and outlet at position 1
are in line. If not , adjust the position of ‘Ring 1’.
7. When both position are in-line; press’2’ . The needle support plate will now move to a position
close to ‘Ring 2’ but should not ‘touch’ or move the blood-switch in any case.
-----------------------------------------------------------------------------------------------------------------------------------
7.4.3. TUBING CONNECTION
To maintain low contamination between the samples and high reproducibility at all time, the blood
tubes must be of the stated type and dimensions. Refer to fig. 23f below.
Note that all whole-blood tubes are of Teflon type 0.7/1.6 mm and fitted to the corresponding
metal connection tubes with Silicon tube 1.0/3.0 mm.
The needle washing device has an inter-change-able in and outlet connection for diluent and waste.
So, both connections are equal. These 2 connections are located near the blood switch.
-----------------------------------------------------------------------------------------------------------------------------------
7.5 MICRO CAPILLARY ADAPTER AD260
The Micro Cap Adapter 260 is an optional device that allows the operator to use direct capillary
blood. Refer to section 6.9 in the User-Manual for detailed user instructions.
The adapter has a built-in start-switch, which is ‘closed’ in case the Cap. Adapter head is removed.
Massive gold contacts are used to allow trouble free operation at all time.
Connection is at connector P52 on the CPU board 530-9001. A jumper on P52 pin 3-4 enables the
CPU to recognize the adapter at power-on.
Therefore, the connection cable at P52 should never be removed if the adapter is integrated in the
CA530.
As the Cap. Adapter is integrated in the flow-system of the CA530, it is important that the head is
not removed without reason. The software will warn the operator with clear text displays in all
possible cases to put the adapter-head back in place. Therefore, no Indications related to the AD260
are available. Only under exceptional conditions if the AD260 head is removed, inserted- removed
during a cycle, some indications might occur. In such case, just restart the CA530.
Picture : AD260 head
Expected service is mainly on the Cap. Adapter head.

Two O-rings, marked as 260-01-014 and 15 in drawing 260-4015 needs to be replaced after c. 1000
capillary samples or one year of operation.
-----------------------------------------------------------------------------------------------------------------------------------
7.6 INSTALLING UPS / MAINS STABILIZERS
The CA530 is, as standard, equipped with an additional input mains filter that suppresses noise in
the low-frequency region. ( 2-200 KHz ) as no standard available mains input filters can handle this
frequency range. This filter gives a minimum of 500V mains noise suppression within this range.
Refer to section 9.5 in this manual for detailed info.
If the CA530 is used in areas with a highly in-stable mains supply or high risk for mains-power
surges caused by lightning; it is highly recommended to use an additional mains-stabilizer.
In case of high mains surges, input ‘transorb’ diodes protect the sensitive electronic parts in most
cases. The motor drivers PBL3773 might be damaged however. Resulting in Indications within the
range of 100-199.
The choice of the mains-stabilizer is an important issue.

In general the only acceptable stabilizer type is a so-called ‘magnetic stabilizer’ . This type consists
mainly of a large transformer runned into saturation. The output voltage is than filtered, usually by
large capacitors and coils, to obtain a sinusoidal voltage. ( max. distorsion 5 % )
These stabilizer types are very common, old fashioned, rugged, no moving parts and cheap.
However they do not supply battery backup in case of a total mains power failure. ( which in most
cases is not necessary)
Using this type of stabilizer, it is important that the ground system of the CA530 is not disturbed.
Below an example is given how to connect the CA530 with all accessories and keeping the ground
system intact. As seen, it is essential that the grounding of the system is at one point ( the
stabilizer ).
This set-up reduces the risk of ‘ground-loops’ which might cause interference and /or damage to
the CA530.
Fig23g
-----------------------------------------------------------------------------------------------------------------------------------
8 PARAMETER FAILURE ANALYSIS
Failure analysis on any parameter within Hematology requires some basic understanding on how
parameters are related and the system flow procedure as described in section 1 of this manual.
The basic measured parameters in any hematology system are always:
RBC, MCV
HGB
WBC
PLT, MPV
All other parameters like HCT & MCHC are derived from the above. In other words an instability
reported on HCT should be analyzed whether this is caused by the MCV or the RBC parameter.
Also, instable MCHC must be analyzed to trace the source to RBC, MCV or HGB as these are the
basic measured parameters.
A bad waste-pump membrane will not empty the mixing cup and /or measuring chambers. This
will be indicated with an indication number within the 200 series and/or bad reproducibility on the
counted parameters RBC, WBC, PLT and HGB.
Size-distribution curves are in general of great use whenever analyzing a system failure.
8.1 HGB INSTABILITY
In case of HGB instability is reported, check if no other parameters are instable as well by first
testing the system on fresh EDTA vein blood. Check especially if the WBC (total) is effected as
well. The Cv of HGB should be less than 1 % within the normal range. An HGB instability will
automatically influence the MCHC parameter as well.
Instability of HGB can be caused by :
a. Bad lyser
b. Instable lamp , or connection.
c. Air bubbles within the final WBC / HGB dilution
d. Leakage in valve 10, 4 or 18
e. Failure in the internal mixing cup ( see section 5.6 )
It is very unlikely that the error is caused by the electronical part. Check service-menu 6.2 and refer
to section 3.2 for detailed info how to analyze the HGB circuit.
-----------------------------------------------------------------------------------------------------------------------------------
8.2 MCV INSTABILITY
In case an MCV instability is reported, check if no other parameters are instable as well by first
testing the system on fresh EDTA vein blood. The Cv of MCV should be less than 1 % within the
normal range and typical less than 0.5%. An MCV instability will automatically influence the HCT
and MCHC parameter as well.
In almost all cases, an MCV instability can be caused by only a few system parameters :
a. Contaminated Diluent
b. Heavy leakage within the piston of the RBC measuring chamber ( section 5.3 )
c. Failure in the RBC orifice ( section 5.5 )
It is very unlikely that the error is caused by the electronical part of the system.
8.3 WBC INSTABILITY
In case an WBC instability is reported, check if no other parameters are instable as well by first
testing the system on fresh EDTA vein blood. The Cv of WBC should be less than 3 % within the
normal range.
Check the size-distribution curve of the WBC parameter. In case it is far shifted to the left, no
reproducible results will be possible.
If there are no HGB discrepances, an instable WBC count can only be caused by :
a. Bad lyser
b. Failure in the WBC orifice ( see section 5.5 )
c. Heavy leakage at the piston of the WBC measuring chamber ( see section 5.3 )
d. Failure in the internal mixing cup ( see section 5.6 )
8.4 RBC INSTABILITY
In case an RBC instability is reported, check if no other parameters are instable as well by first
testing the system on fresh EDTA vein blood. The Cv of RBC should be less than 1 % within the
normal range.
As the RBC is derived from the second dilution, any failure within the first dilution will also affect
the RBC parameter. In such a case, the system error should be traced within the first dilution.
In other words, if an RBC AND an HGB instability is reported, the fault should be within the first
dilution. The cause might be :
a. Leakage in valve 9, 8 or 6
b. Mixing cup ( see section 5.6 )
c. The Teflon tube of the aspiration pipette is not fitted correctly at pos.1 of the turning valve.
Check that the Teflon tube is as far as possible inserted within the turningvalve connection at
pos. 1
In case the HGB parameter is stable, the failure might be caused in :

a. Leakage in valve 9 or 15.
-----------------------------------------------------------------------------------------------------------------------------------
8.5 PLT INSTABILITY
In case a PLT instability is reported, check if no other parameters are instable as well by first
testing the system on fresh EDTA vein blood. The Cv of PLT should be less than ( typical ) 3.5 %
within the range 250 - 550 .
If a too large floating discriminator range is selected, PLT reproducibility might suffer especially
when low PLT values are reported. This is a logical behavior as with low concentrations, low
statistical accuracy is obtained within the size-distribution curve.
Good reproducible results are always obtained when setting the floating discriminator to a fixed
level at ca. 25 fl. However, the system might report erroneous results on microcytic cell
populations instead. Therefore, a floating discriminator is recommended.
If the above conditions are met, instability might be caused by :
a. Contaminated Diluent ( high background count )

b. Mains-supply noise ( see section 3.6 )
c. Failure in the RBC orifice ( see section 5.5 )
d. Contaminated mixing cup ( see section 5.6 )
-----------------------------------------------------------------------------------------------------------------------------------
9 DESCRIPTION ELECTRONICS
The description of the PC-boards is found in this chapter. The circuit diagrams are , for clarity
purposes, devided in ‘sheets’ with their corresponding description.
Note that C-MOS circuits are used, therefore be aware of static electricity whenever changing
components. Component exchange is not recommended without the written instructions from
Medonic; the below descriptions are for reference purposes only.
The boards have a basic numbering system found on the top screening and start with 530-90xx .
Board version numbering is found on the physical toplayer and starts with 530-70xx.
E.g. the board screen text 530-9001 is allocated to the main cpu board. The text found on the
physical top-layer will be in the range 530-7001 to 530-7009 dependent on the lay-out version.
For backwards / forward compatibility’s; refer to the technical bulletins concerning the CA530 as
supplied by Medonic.
9.1 CPU BOARD 530-9001
Sheet 1
The microprocessor IC-7 has a buffered data / address bus found in IC9,11,6 and 13. The clock-
oscillator is formed by an integrated module X1. The reset is provided through a watchdog circuit
as found in sheet 11 , IC3.
Sheet 2
The memory lay-out of the main processor is formed by 2 RAM circuits ( IC 8 and 5 ) and 2
EPROM’s where the CA530 program is stored.
The RAM circuits are battery backed-up, see sheet 11 for details.
Program version numbering is done in the following way :
530-70-188 and 530-71-188 where 188 stands for the version number.
‘70’ means the EVEN position equal to IC12 and ‘71’ the ODD position, equal to IC1.
(i.e. x0 is always EVEN and x1 always ODD )
Please note that :
a. 70 and 71 is assigned for 50 and 60 Hz operation.

b. 50 and 51 for 50 Hz operation only
c. 60 and 61 for 60Hz operation only
-----------------------------------------------------------------------------------------------------------------------------------
Sheet 3
IC 18 is the signal processing Gate-Array-Logic. Differences between the Thor, Oden and Mimer
are stored here and NOT in the EPROM’s. All logical signal-processing operations are handled by
this circuit.
Sheet 4
The peak-detectors and A/D conversion is described here. Two detectors are available for the RBC
and WBC channel. Cell_0 stands for the RBC channel and Cell_1 the WBC channel. IC 50 and 51
are the peak-detectors and IC37 and IC38 the corresponding A/D converters, converting each puls
into a 12 bit digital value, read on the data-bus into a DMA channel of the uP.
IC 52 forms the hard-ware discriminator. If an input signal is larger than the preset voltage formed
by the resistors R82,87,88 and 89 , IC 52 is triggered and the A/D conversion is started.
IC44 is a buffer for the reference voltage, derived from the internal voltage reference of the A/D
converter IC 37.
IC29 and IC48 , R78, C110, R77, C111 forms a puls-width modulated digital to voltage converter
for the HGB lamp. The lamp, connected to P41, is driven by IC45 ( buffer ) and Q2 ( final power
driver transistor )
Sheet 5
The valve driver circuits IC35, 39, 40, 41, 43, 46 and 49 are driven from the PIO circuits IC30 and
IC31. An active valve is shown with a corresponding LED lit as found in LD10 to LD32.
Note that not all valve outputs are used.
D2 ( POP ) is connected to the power-supply board. Each time a valve is closed, the POP line is
pulsed to increase the valve voltage to ca. 28 Volts.
IC30 is also used to sense / drive several start-switches as well as the waste-pump.
Sheet 6
The motor circuit for the syringes and turning valve is formed by IC16, 17 and the final drivers by
IC15 and IC22.
P60 is connected to the syringe motor and P60 to the turning valve.
-----------------------------------------------------------------------------------------------------------------------------------
Sheet 7
The start and stop detectors are found in the circuit connected to P73. The potentiometers RV1-
RV4 are described in section 3.3 of this manual. The comparators IC26 and IC23 are only used to
drive the corresponding LED’s for adjustment purposes. The actual 0 / 1 level is connected to the
uP via IC29.
The testpoints TP2-TP5 will show a voltage of > 4 Volts if air in the metering unit and < 0.5 Volt if
the metering unit is filled with liquid. Note that the LED’s (driven by VCC and the resistors R29,
36, 50 and 52) in the metering unit ONLY are activated during a count cycle or when activating
program 6.3 in the service-menu.
The bottle detectors are connected via P75 to a comparator IC25 and IC28. A puls of ca. 50 usec is
supplied on terminals P75-1, 4 and 8 and the return voltage is measured and compared by a fixed
voltage on the comparators formed by R46 and R48.
The output DET_0 to DET_2 is connected to the processor circuit.
IC32 ( PIO ) is connected to the EEPROM IC33, where all instrument calibrations are stored, and
the real-time clock IC34.
Sheet 8
IC42 is a multi-channel serial A/D converter used to measure the HGB voltage from the analogue
amplifier board ( HGBSIG ) and the position of the turning valve connected to P82.
P83 is unused in current versions.
P81 is connected to the blood-detector as described in section 3.4 . The sensitivity of the blood-
detector is controlled by the current through the LED adjusted with RV5. The output voltage of the
blood-detector photo-transistor is measured by the serial A/D converter IC42 .
IC4 is the PIO used for the keyboard scanning. The keyboard has no additional logic drivers for the
key’s. IC10 is the ‘scan-driver’ , the return signal is directly connected to the PIO IC4 and
decoding takes place within the software.
Sheet 9
The serial output driver IC20 supplies a RS232 compatible output on the 9 pin D-SUB P92. Note
that jumper J2 must be in place to provide a correct grounding.
IC20 can be replaced by a fully galvanic isolated RS232 driver if necessary. This device is
available from Medonic on request ( IC21 ). In case such a driver is installed, the jumper J2 must
be removed as well as IC20.
-----------------------------------------------------------------------------------------------------------------------------------
Sheet 10
The auxiliary motor drivers are all ‘zero-crossing’ triac circuits driven by opto coupled circuits to
avoid large voltage transients within the system. The waste-pump is driven by the triac Z8 which is
a 25 Amp device. The typical waste-pump current handled by Z8 is ca. 3.5 Amp (range 3 to 5
Amp)
Z4 and Z3 are drivers for the optional closed-tube adapter.
Sheet 11
IC3 is the overall watchdog circuit that handles the reset of the uP. The battery backup driver for
the RAM circuits, which handles the patient sample data memory, is integrated within this circuit.
Note that jumper J1 is enabling the battery back-up of the system. If J1 is removed, no sample data
is kept stored during a power failure.
This sheet provides also a list of voltage supply pins of most of the circuits used on the main CPU
board.
Measured with GND as reference unless other stated
a. VCC 4.9- 5.2 volt ( stabilized )

b. Vrelay 21 to 28 volt (stabilized )
c. B200V 150 volt ( non-stabilized )
d. CAPV 17.8 - 18.5 volt (stabilized )
e. VSM 35 volt ( non stabilized )
f. ACAC1-2 25 volt AC ( non stabilized ) , measure between ACAC1 and ACAC2
g. V+ 14.7 - 15.3 volt ( stabilized )
h. V- - 14.7 - 15.3 volt ( stabilized )
I. ACDC - 4.7 - 5.3 volt ( stabilized ) , measure between ACDC and ACAC2.
-----------------------------------------------------------------------------------------------------------------------------------
9.2 POWER-SUPPLY BOARD 530-9011
The power supply board is of an analogue type. No switching modules are used.
The grounding system is carefully designed as seen on the schematic diagram. External voltage
stabilizers are mounted on the rear-side of the CA530 ( isolated from the chassis ).
IC-0A ( LM338K ) is used to stabilize the valve voltage ( 21 to 28 Volt )
IC-0B ( LM 340 AK ) is the VCC stabilizer set at 5.2 volts.
These stabilizers have the following wire-coloring :

Reference Color
IN Red
OUT Blue
ADJ/GND Black
The connections from the transformer to the power-supply board are as follows :
Board-reference Approx. voltage (AC) Color Used by
X1-X3 24 Volt Yellow Valve driver / Stepping motors

X2-X4 24 Volt White AC Motors ( e.g. Waste-pump )
X6-X8 110 Volt Brown Orifice system
X7 Mid-point of X9 and X10 Red Analogue circuits
X9-X10 2 x 18 Volt in respect to X7 Green / Blue Analogue circuits
X11-X12 10 Volt Violet VCC digital circuits
Voltages measured at the stated testpoints :

Testpoint Reference Nominal (V) Tolerances Used by
TP1 TP2 5.1 4.9-5.35 Digital circuits

TP3 TP2 35 33-38 Valve drivers
TP6 TP5 -5.0 -4.7 - 5.3 Motor driver
TP11 TP2 15.0 14.7 - 15.3 Analogue circuits
TP12 TP2 -15.0 -14.7 - 15.3 Analogue circuits
TP10 TP2 150 130 - 165 Orifice system
TP13 TP2 18.1 17.8 - 19.0 Orifice system
TP7 TP5 24 AC 23 - 28 AC Motors
TP9 TP2 21-29 20-22 Valve voltage *
27-29.8
* A voltage of nominal 21 Volt is measured if TP14 is ‘high’ ( > 5 Volt )

A voltage of nominal 29 Volt is measured if TP14 is ‘low’ ( < 0.5 Volt )
Fuse F1 is rated 5AT ( valve power-supply ) size 5 x 20 mm

Fuse F2 is rated 6.3 AT ( AC motor supply, waste pump ) size 5 x 20 mm
-----------------------------------------------------------------------------------------------------------------------------------
9.3 ANALOGUE AMPLIFIER BOARD 530-9021
The analogue amplifier board is shielded with the analogue grounding system separated from the
chassis ground. It is essential that this board, whenever removed / changed, is re-mounted in the
original state with the shield as well as the cover, and isolated from the bottom chassis.
The analogue board consists of 2 identical analogue amplifiers for the RCB and WBC channel,
although different gain settings are used.
The HGB photo-diode amplifier is also located on this board.
Sheet 1
This page shows the 2 analogue amplifiers of the orifice system. The orifices are physically
disconnected from the orifice voltage supply, if no sample is processed, by Relay’s RE1 and RE2
The input amplifier is protected against over-voltages by the diodes D3-D6 ( RBC )
Amplification ( RBC side ) is done by IC3 and IC4 acts as an active filter.
DC restoring takes place in the circuit around IC4-B. The outputs are market Cell_0 (RBC) and
Cell_1 (WBC).
The gain of the amplifiers is set by RV2 ( RBC ) and RV4 (WBC).
Note that any change of these potentiometers will cause a MCV change ( RV2 ) or change the
differential acceptance range of the WBC diff. ( RV4 ).
Trimpotentiometer RV3 is set to zero-volt output at TP 4 with the coaxial cable from the orifice
disconnected.
The amplifier outputs can be reached without dismantling the cover-shield.

The top-shield should not be removed when monitoring the output signals on an oscilloscope at
testpoints TP4 ( RBC ) or TP5 ( WBC ). The oscilloscope grounding must be connected to the
board-shield and not to the chassis.
See figure 24 below:
A peak voltage at TP4 of 5 Volt is equal to 250 fl for RBC and 400 fl for WBC when measured at
TP5.
Photom. adjust. Coax Photom.
TP4 (RBC output)

RBC Coax
TP6 (WBC output)

WBC Coax
PCB 530-9021
Fig 24
-----------------------------------------------------------------------------------------------------------------------------------
Sheet 2
This pages shows the HGB analogue amplifier. For gain adjustments, please refer to section 3.2 of
this manual. The input amplifier is a current to voltage amplifier with high gain. The output voltage
can be measured at TP2, however it is easier to monitor this voltage within service-menu 6.2 as this
point is directly connected to the internal A/D converter and it’s value is shown in this menu.
9.4 DISPLAY / KEY-BOARD 530-9032
The display and key board have no additional IC circuits. RV1 is used to adjust the viewing angle
of the display and is reachable without removing the shield-cover. Note that this shield must be in
place, else high PLT background problems might occur.
9.5 INPUT MAINS FILTER BOARD 530-9040
The mains voltage is filtered on this board. This input filter ( included the total load of the CA530 )
performs an effective filtering characteristic within the range 2- 200 Khz.
Within this frequency range, often triac switching distortion is found. As many standard filters do
not supply an effective filtering within this frequency spectrum, Medonic has chosen to supply all
CA530’s with this specific filter.
A nominal input interference source of 700 Volts within a spectrum range up to 2 Ghz is
effectively filtered by this board. Interference over this (amplitude) level will first introduce high
PLT backgrounds and finally, at a level of ca. 3 to 4 KV, reset the system via the watchdog.
The mains-ground is included in the filter as well.
The Transorb diodes marked ‘D’ have effective surge capabilities. They protect the instrument
against lightning as well. In case a secondary lightning should strike the mains-supply, these
diodes will be shortened within a few nano-seconds and than blow the fuses. In such a cases, these
diodes needs to be replaced.
This board provides the input voltage range switching as well. Please refer to fig 25 / 26 below to
switch the CA530 to other mains-voltages supplies.
Note that the main fuses needs to be replaced by 4 AMP ( AT types ) whenever using the
instrument on 120 Volt supplies or lower.
The transformer used in the CA530 has an internal temperature protection. In case abnormal
overheating should occur, the primary side of the transformer is disconnected from the mains
supply by the internal temperature protection circuit.
-----------------------------------------------------------------------------------------------------------------------------------
AC Output
220 Volt connection
w n e
o w g
n kc
le o a te a d
e rB r
O lB e
Y u l
o R
p l
B V V i
V V V
m 0 0 0 0
e V 0 2 V 0 2
T 0 1 1 0 1 1
Jumper
AC Output
240 Volt connection
w n e
o w g
n kc
le o a t a d
e rB r
O e l
B e
R
Y u l
o
p l
B V V iV V V
m 0 0 0
e V 0 0
2 V 0 2
T 0 1 1 0 1 1
Jumper
Fig 25
-----------------------------------------------------------------------------------------------------------------------------------
AC Output
100 Volt connection
w n e
o w g
n kc
le o a t a d
e r
B rO e lB e
Y u l
o R
p l
B V V iV V V
m 0 0 0 0
e V 0 2 V 0 2
T 0 1 1 0 1 1
2 Jumpers
AC Output
120 Volt connection
w n e
o w g cka
le o n d
r
B a t
e l e
Y e r
O B R
p u V l
o V
m lB 0 V iV 0 V
e V 0 0 V 0 0
2
T 0 1 2 0 1 1
1
2 Jumpers
Fig 26
The mains supply of the CA530 will handle a range of 175 to 240 Volts ( if connected as a 220
Volt unit ) without any problem. Voltages lower than 175 Volt might introduce PLT background.
At ca. 150-155 Volt, the system watchdog reset will be activated and introduce indication numbers
in the range of 300-399.
If , by some reasons, a filtered ground is not allowed due to local electrical safety regulations;
connect the input ground wire ( yellow / green ) directly to the chassis.
See also : Appendix / Technical Bulletins , if available
-----------------------------------------------------------------------------------------------------------------------------------
11 SERVICE SCHEDULE
Several parts in the CA530 need regular service. As whole-blood is used, certain cleaning
procedures are obvious. Below follows a service schedule that is recommended to avoid unplanned
service visits to the enduser. The schedule assumes that the cleaning procedures as described in the
user manual of the CA530 are followed by the enduser. If this is not the case, these 'service' points
should be added to this list.
Assumed is ca. 50 samples/day, if a higher load is used; adjust the time-frames in the service
schedule according to this.
Note that some parts might have a much longer lifetime than 'stated' in the service manual.
However the service sched's should be seen as a way to avoid unplanned service visits as much as
possible.
After the 24 month period, a service time-frame of 12 months is recommended
Below follows a short reference to the stated service points :
Note :
Be always aware of infection risks during service of the instrument. As whole blood is used,
care should be taken to minimize any infection risk.
Use disinfection-solutions as supplied by the lab staff and follow the usage instructions of
qualified lab. personal. (Hypo-chlorite can be used as disinfection-solution without any harm
to the instrument.)
-----------------------------------------------------------------------------------------------------------------------------------
11.1 SERVICE POINTS
PNT 1 START/STOP
The START/STOP detection is one of the most essential points in the CA530.
Note that an adjustment is normally not necessary, only a check should be done to be sure that the
start/stop system is correct..
Don't adjust these detectors if not necessary.
Refer to section 3.3 for detailed information
-----------------------------------------------------------------------------------------------------------------------------------
PNT2 PHOTOMETER
An installation check is recommended concerning the gain settings of the photometer system.
Perform an auto-adjustment by pressing digit ‘1’ within service-menu 6.2 after the instrument is
installed at the end-user.
Please refer to section 3.2 for detailed information.
Note that any further adjustment should not be necessary, only a check should be done to be sure
that the photometer system was not effected by the transport of the instrument to the end-user.
Proceed as follows during the 6/12 month service check
The cleaning of the WBC measuring chamber should be performed as described in section 3.3
including an auto-adjustment within service-menu 6.2
Refer to section 3.3 for detailed information concerning photometer adjustments.
PNT3 MEMBRANE
The membranes located in the waste-pump have to be changed. Remove the housing of the pump
section of the waste-pump and change the membrane/package.
If the capacity of the waste-pump is too low, indication 202 or 204 might occur.
PNT4 CHECK /LEAKAGE
Check that there are no leakage's within the CA530. Look for salt crystals. If found, remove any
salt crystals with dist water only. Salt crystals that could fall on tube-valve plungers, might
introduce unstable functioning of these valves.
· The CA530 should always be clean around the tube-valves to allow a trouble free operation
of the valve plungers.
-----------------------------------------------------------------------------------------------------------------------------------
PNT5 CLEANING
Cleaning sequences that are not mentioned in other service points, are mentioned below:
1. Remove the mixing-cup with the 2 screws and clean BOTH the cup/electrodes and lid with dist.
water only. Rinse all electrodes on the inside with a pin/wire of 0.8 mm.
2. Clean the aspiration pipette with alcohol ONLY on the outside
3. Check the waste outlet tube, change if excessive bacterial grow is found at the waste outlet/tubes
or any other device that is connected at the waste of the CA530.
4. Put both probes (Diluent and Lyzer ) in the enzymatic cleaner ‘ProClean’ . Perform a ‘FILL
SYSTEM’ and wait c. 20 minutes.
5. Fill the CA530 with the original reagents.
PNT6 TUBES
The silicon tubes used in the tube valves have a limited life-time. With the stated
number_of_samples/day ; they should be replaced each 2nd year.
Replace only the tubes that are actually going through the valves.
Replace tubes that are obvious damaged by any other reason.
Please refer to section 5.4 for detailed information
PNT7 PISTONS
The syringe pistons should be exchanged whenever excessive liquid is found on the cylinder walls
below the pistons. Leakage’s will directly influence the MCV parameter.
An adjustment as described in section 5.3 might stop a piston leakage temporary. A piston
replacement is recommended only in case of an obvious leakage.

-----------------------------------------------------------------------------------------------------------------------------------
PNT8 BLOOD DET.
The blood detection level might change by time due to protein layers in the (Teflon) aspiration
tube. Also, a protein layer will decrease the drying efficiency of the aspiration pipette, resulting in
slight 'carry-over' tendencies.
Proceed as follows:
1. Enter an enzymatic cleaner or 4-5 % hypo-chlorite as a sample and run ca. 5 cycles.
2. Refer to section 3.4 to check/adjust the blood detectors.
Note that the actual 'trigger' level is not critical. As long as 'blood' with an HGB of 0.5 g/dl is not
detected as blood and 'blood' with an HGB of ca. 2 g/dl is detected as blood; the detectors are
correct and no further adjustments are necessary.
PNT9 CT220 NEEDLE
The needle in the Cap Piercing Device needs basically no regular service. In case of operator
mistake or heavy clogging tendencies due to blood coagulation, the needle needs to be replaced. It
is therefore recommended that at least one needle is kept at the end-user as a spare-part in case of.
Refer to the User-Manual section 6.8.4 for detailed information.
PNT10 AD260 O-RINGS
The 2 O-rings marked 15 and 14 in the attached drawing 260-4015 needs to be replaced after c.
1000 samples or one year of operation. In case of a leakage, salt crystals might contaminate the
device, resulting in high WBC background counts.
At least one set ( 2 ) of O-Rings should be at the end-user, as they can be replaced without external
service assistance.
-----------------------------------------------------------------------------------------------------------------------------------
12 APPENDIX / TECHNICAL BULLETINS
Use this section to collect additional information send by Medonic as Appendix and/or Technical
bulletins / Document.
Medonic document numbering system :
530-30-2XX Appendix user manual (version 2)
530-31-2XX Appendix service manual (version 2)
000-80-xxx General service bulletins
Note that most info in these bulletins is not found in any other section of this service manual !
13 REPORTING SERVICE QUESTIONS
Please use a copy of the attached form whenever contacting Medonic for further service
information.
As the CA530 is equipped with an important service tracking system; enclose always the
printouts of:
a. 'PRINT ALL SETTINGS' ( Setup-Menu )
b. ´'PRINT MACHINE STATISTICS' directly after running the sample where the problem was
found. (Service menu )
c. Some samples including the size-distribution curves where the problem is observed.
-----------------------------------------------------------------------------------------------------------------------------------
To: Boule / Sweden From :

Att: Customer Technical Support Att :
Subject : CA530/620 Service

Serial Number :
Approx. date of installation :
Diluent type used:

Batch # :
Lyser type used :

Batch # :
Description of the problem:
Attached: Copy of ‘Print all settings’

Copy of ‘Machine statistics’
Copy of Samples including size-distribution curves
14 ORDERING SPARE PARTS
The enclosed exploded view consists of a positioning numbering system.

For example, the syringe motor of the CA530 has a position number 530-02-067
Look in the general Medonic spare-part list if this position number is listed as a general spare part,
in such case it will refer to a ‘part-number’.
If so, order the item by the ‘part-number’.
If no part-number is supplied within the general spare-part list; it indicates that this part is not
considered as a ‘ common spare part’ .
Such part can be ordered anyhow by referring to the position number only.
<EOF>

Service - Manual Medonic 520

Uploaded by

Copyright:

Available Formats

You might also like

Service - Manual Medonic 520

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Service - Manual Medonic 520

Uploaded by

Copyright:

Available Formats

MEDONIC CELLANALYZER CA530 Version 2.

3 SERVICE MENU’S / SPECIAL PROCEDURES.............................................................................................................19

6 TUBING SYSTEM LAY-OUT...........................................................................................................................................57

13 REPORTING SERVICE QUESTIONS.............................................................................................................................86

14 ORDERING SPARE PARTS..............................................................................................................................................87

0.1 COPYRIGHT () NOTICE

All rights strictly reserved. Reproduction or issue of this

This copy issued by :

0.2 SERVICEMANUAL CHANGES/UPDATES

Filename of this service manual : 530-31-200

Filename of user manual : 530-30-200

Document 530-31-201 Software update history

This section is reserved for errata corrections.

1 FUNCTIONAL DESCR. FLUIDIC SYSTEM

1.1 INTRODUCTION FLUIDIC DIAGRAM / GLOSSARY

Pinch valves are of 2 types.

A single valve is a normally closed valve and has the symbol :

A two-way valve has a normally closed and a normally open section.

Position 'A' Position 'B'

Syringe / measuring chamber

1.2 WHOLE BLOOD ASPIRATION

1.3 FIRST DILUTION

1.4 MIXING THE FIRST DILUTION

1.5 PREPARING THE FINAL DILUTION

1.6 CLEANING THE ASPIRATION PIPETTE

1.7 FINAL DILUTION

1.8 START ANALYZING PROCESS

1.9 CLEANING THE MEASURING CHAMBERS

1.10 FINAL POSITION

2.0 PREDILUTED SAMPLES FLUID DIAGRAM

Samples that are prediluted are aspirated though valve 7a.

3 SERVICE MENU’s / SPECIAL PROCEDURES

3.1 VALVE / WASTE-PUMP CHECK

Enter service-menu 6.1 as shown below :

6.1 PUMP&VALVE TEST / TO SELECT 0

3.2 PHOTOMETER CHECK / ADJUSTMENT

6.2 LAMP= 4.70 V PHOTOCELL =3.50 V

WASTE OUTLET WBC

Do as follows in menu 6.2

a. Put the lamp-voltage at 4.60 volt with the / keys

On each sample the HGB is calculated as :

HGB = F* Log ( Blankvoltage-Offsetvoltage / Samplevoltage-Offsetvoltage)

3.3 START / STOP DETECTOR CHECK / ADJUSTMENT

6.3 START/STOP DETECTOR STATUS

Do as follows to adjust the start and / or stop detector :

a. Enter the service menu 6.3

Start / Stop Trimpot. LED showing liquid LED showing air

WBC-L RV4 LD2 LD1

3.4 BOTTLE DETECTORS & BLOOD DETECTOR ADJUSTMENT

6.4 BOTTLE STATUS

A ‘1’ shows reagent present, a ‘0’ means ‘bottle empty’.

Checking / adjusting the blood detector

The total range of this voltage is 0-4.1 Volt

e. Turn trimpotentiometer RV5 on the CPU board so that 3.5 V is read

3.5 TURNING VALVE & PISTON DETECTOR CHECK

6.5 MOTOR DETECTORS

3.6 MAINS SUPPLY NOISE TEST

6.6 AMPL. FREQ. AMPL. FREQ.