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Home › Life Sciences › Cell Analysis › Flow Cytometry › Flow Cytometry Assays and Reagents › Immunophenotyping Kits for Flow Cytometry

Immunophenotyping Kits for Flow Cytometry

‹ Flow Cytometry Assays and Reagents
Optimized flow cytometry assays to detect
Apoptosis Assays for Flow Cytometry
immune cells
The Invitrogen eBioscience essential phenotyping kits are an efficient and cost-
Cell Cycle Assays for Flow Cytometry
effective flow cytometry assays. These ready-to-use kits have been designed for
Cell Proliferation Using Flow Cytometry the reliable identification of T cell subsets including CD4+, CD8+, and regulatory
T cell (Tregs) in human blood.
Cell Viability Assays for Flow Cytometry

Ready-to-Use Flow Cytometry Reagents Instrument agnostic—antibodies, protocol, and gating strategy are
compatible with any flow cytometer instrument equipped with violet 405 nm,
RNA Detection By Flow Cytometry
blue 488 nm, and red 633 nm lasers
Microbiology Assays for Flow Cytometry Comprehensive analysis strategy—a standardized data acquisition and
analysis plan is provided to perform flow cytometry characterization
5 Steps to Intracellular Flow Cytometry
Optimized antibody and reagent concentrations—panels contain the best
Immunophenotyping Kits for Flow combinations of antibodies and dyes for maximum sensitivity from a
Cytometry multicolor fluorescence experiment

Need something unique? You can easily incorporate or design a customized

panel with our technical specialists.
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Workflow

Step 1 Step 2

Stimulate cells from peripheral mononuclear cells
(PBMCs), lysed whole blood samples, or single-cell
suspensions from human tissue for protein expression.
This step is only required for eBioscience Essential
Human Th1/Th17 Phenotyping Kit.
Use buffer set included in kit for optimal intracellular
staining.
Add LIVE/DEAD reagent to gate out debris and
artifacts.
Add labeled antibodies against cell surface and
intracellular markers to enable cell identification and
functionality characterization.

Click image to enlarge

Step 3
Step 4
Use instrument and compensation beads to set up flow
cytometer for optimal cell population determinations. Set voltages and suggested gating strategies for cell
analysis.

Figure 1. Overview of the T cell subset workflow. No extensive panel design is needed. You can just follow the protocol to generate your data.
The gating strategy gives guidance to find the correct cell populations when acquiring events. Results will show the percentage of T cells positive
or negative for the indicated markers.

Kits for T cell immunophenotyping

Validated antibody flow panels
T cell Treg Th1/Th17
CD3:APC-eF780 CD4:FITC CD4:FITC
CD4:APC CD25:PerCP-eF710 IL-17:APC
CD8:eF450 CD127:PE IFN-γ:PE
CD62L:FITC Foxp3:eF450
CCR7:PE

Figure 2. T cells can be categorized into subset populations based on the particular immune response(s) they mediate. CD8+ T cells
directly kill infected or tumor cells via perforin and granzymes. CD4+ cells regulate other immune cells through adaptive immunity responses.
CD4+ cells can be stimulated to differentiate into multiple populations: for example, resulting Th1 cells influence macrophage and B cells and
resulting Th17 cells secret cytokines and other factors for an inflammatory response. The Treg population inhibits or downregulates the immune
response.

Instrument specifications Data

Instrument specifications required for analyzing eBioscience phenotyping kits

To run panels within these kits, instruments are required to have a 2–3 laser system with flexible filter configurations.
Below is an example instrument configuration achievable for most traditional flow cytometers.

Table 1. Example instrument specifications

Instrument Specification
needed run an eBioscience Essential

Flow cytometer Lasers: Phenotyping Kit.

Violet (405 nm)

Blue (488 nm)
Red (633 nm)

Filter sets:
eFluor 450 Pac Blue: 440/50 nm
eFluor 506: 510/50
PerCP-eFluor 710: 710/50 (695/40 acceptable)
FITC: 530/20
PE: 574/26
APC: 660/20

The Applied Biosystems PureQuant Assay

Facilitates regulatory filings—shorten development timelines; established performance specifications following
ICHQ2(R1) guidelines
Proven technology—used for immune cell identification and quantification for over a decade
Easily standardized—helps enable consistent results across multiple users, instruments, and sites
Scheduling flexibility—run fresh cells, frozen cells or gDNA at your convenience

Learn more about PureQuant Assays

Antibodies Cell therapy systems

Find antibodies for flow cytometry › Products for immunotherapy ›
Get help building a custom panel › Cell Therapy Systems (CTS) support ›

Catalog # Name Size Price (USD) Qty

eBioscience™ Essential
A42923 100 reactions Contact Us › -
Human T-Cell Phenotyping Kit

eBioscience™ Essential
A42925 100 reactions Contact Us › -
Human Treg Phenotyping Kit

eBioscience™ Essential
A42927 Human Th1/Th17 Phenotyping 100 reactions Contact Us › -
Kit

Resources Support
Flow Cytometry Learning Center—Access flow Flow Cytometry Support Center—Find technical support
cytometry educational resources for better experiment recommendations for your flow cytometry workflows,
planning and execution. including tips for experimental setup and in-depth
troubleshooting help.
Flow Cytometry Panel Builder—Design your flow
cytometry panel with this online tool for a simplified, Flow Cytometry Protocols ›
customizable experience to fit your needs.
Contact us ›
5 Steps Resources
5 Steps to Intracellular Flow Cytometry

5 Steps to Publication-Quality Fixed Cell Imaging

See all 5 Steps Workflows

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

For Research Use Only. Not for use in diagnostic procedures.

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