EXPERIMENT NO.

8
QUALITY CONTROL TESTS OF IODINE TINCTURE
PRODUCT DESCRIPTION
GENERIC NAME: Iodine Tincture
BRAND NAME:
VOLUME; 25 ml
STRENGTH: 2% Iodine
MANUFACTURER: Spectrum Laboratory PVT. Lahore
BATCH NO. 107
ALCOHOL CONTENT: 40%-50%

ALCOHOL CONTENT DETERMINATION

APPARATUS:
Measuring cylinder, stirrers, thermometer, pipette, weighing balance, distillation apparatus, and
pycnometer

CHEMICALS:
Tincture iodine, distilled water, powdered zinc or sodium thiosulphate 10%, sodium hydroxide
TS.

SPECIAL TREATMENT:
Treat all solutions containing free iodine with powdered zinc before the distillation or decolorize
with just sufficient sodium thiosulfate solution (1 in 10), followed by a few drops of sodium
hydroxide TS.

PROCEDURE:
By means of a pipet, transfer to a suitable distilling apparatus not less than 25 mL of the liquid in
which the alcohol is to be determined and note the temperature at which the volume was measured.
Dilute the specimen with about twice its volume of water, collect a volume of distillate about 2
mL less than twice the volume of the original test liquid, bring to the temperature at which the
original liquid was measured, add sufficient water to measure exactly twice the original volume of
the test liquid and mix . The distillate is clear or not more than slightly cloudy and does not contain
more than traces of volatile substances other than alcohol and water. Determine the specific gravity
of distillate.
DETERMINATION OF SPECIFIC GRAVITY
Select a scrupulously clean, dry pycnometer that previously has been calibrated by determining its
weight and the weight of recently boiled water contained in it at 25°. Adjust the temperature of the
liquid to about 20° and fill the pycnometer with it. Adjust the temperature of the filled pycnometer
to 25°, remove any excess liquid, and weigh. When the monograph specifies a temperature
different from 25°, filled pycnometers must be brought to the temperature of the balance before
they are weighed. Subtract the tare weight of the pycnometer from the filled weight. The specific
gravity of the liquid is the quotient obtained by dividing the weight of the liquid contained in the
pycnometer by the weight of water contained in it, both determined at 25°.
Using this result to ascertain the percentage, by volume, of C2H5OH contained in the elixir
examined by reference to the Alcoholometric Table. The proportion of C2H5OH, by volume, in
this distillate, as ascertained from its specific gravity, equals one-half that in the liquid examined.
PRECAUTIONS AND REQUIREMENT FOR DISTILLATE COLLECTION:
• The capacity of the distilling flask is sufficient (commonly two to four times the volume
of the liquid to be heated) and the rate of distillation is such that clear distillates are
produced.
• Cloudy distillates may be clarified by agitation with talc, or with calcium carbonate, and
filtered, after which the temperature of the filtrate is adjusted, and the alcohol content
determined from the specific gravity.
• During all manipulations, take precautions to minimize the loss of alcohol by evaporation.
• Treat liquids that froth to a troublesome extent during distillation by rendering them
strongly acidic with phosphoric, sulfuric, or tannic acid, or treat with a slight excess of
calcium chloride solution or with a small amount of paraffin or silicone oil before starting
the distillation.
• Prevent bumping during distillation by adding porous chips of insoluble material such as
silicon carbide, or beads.

ASSAY OF IODINE TINCTURE

APPARATUS:
500 ml volumetric flask, burette, dropper, pipette, beaker

CHEMICALS:
Iodine tincture, distilled water, 0.1N sodium thiosulphate, starch TS

PROCEDURE:
Transfer 10ml of topical solution into a glass-stoppered 500ml flask and dilute with 10ml of
water. Titrate with 0.1 N sodium thiosulphate VS, adding 3ml of starch TS as the endpoint
approached. Each ml of 0.1N sodium thiosulphate is equivalent to 12.69 mg of Iodine.
Acceptance Criteria: Each 100ml of Iodine tincture should contain Iodine (1.8g-2.2g)