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  • Essentials of Biophysics: Topic: Blotting Techniques

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    This document provides an overview of blotting techniques, including: 1) It describes three main blotting techniques - Southern blot, Northern blot, and Western blot. 2) It summarizes the origins and inventors of each technique, including that Southern blot was invented by Edwin Southern in 1975, Northern blot was developed by David Kemp and James Alwine in 1977, and Western blot was originated in Harry Towbin's laboratory in 1977-1979. 3) It clarifies some of the history around the development of Western blot, noting debates around whether it was first developed by Burnette, Towbin's group, or Stark's group.

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    1602329963_BM_501_Module_01_Blotting

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    This document provides an overview of blotting techniques, including: 1) It describes three main blotting techniques - Southern blot, Northern blot, and Western blot. 2) It summarizes the origins and inventors of each technique, including that Southern blot was invented by Edwin Southern in 1975, Northern blot was developed by David Kemp and James Alwine in 1977, and Western blot was originated in Harry Towbin's laboratory in 1977-1979. 3) It clarifies some of the history around the development of Western blot, noting debates around whether it was first developed by Burnette, Towbin's group, or Stark's group.

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    34 views21 pages

    Essentials of Biophysics: Topic: Blotting Techniques

    Uploaded by

    sampot
    This document provides an overview of blotting techniques, including: 1) It describes three main blotting techniques - Southern blot, Northern blot, and Western blot. 2) It summarizes the origins and inventors of each technique, including that Southern blot was invented by Edwin Southern in 1975, Northern blot was developed by David Kemp and James Alwine in 1977, and Western blot was originated in Harry Towbin's laboratory in 1977-1979. 3) It clarifies some of the history around the development of Western blot, noting debates around whether it was first developed by Burnette, Towbin's group, or Stark's group.

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    of 21

    MIT School of Bioengineering Sciences and Research

    (A Constituent unit of MIT ADT University)

    ESSENTIALS OF BIOPHYSICS
    COURSE CODE: BM501

    Module: 01
    Topic: Blotting Techniques

    Saturday, 10 October 2020 MIT-ADT University/MITBIO/BM501/Dr. Preetam Bala 1


    Modules
    M: Modules
    Blotting M1

    Chromatography M2
    Separation • History
    techniques
    BIOPHYSICS
    Electrophoresis M3 • Concept
    • Principle
    Centrifugation M4
    • Types
    X Ray M5
    Crystallography • Methodology
    Analysis • Applications
    Spectroscopy M6
    techniques
    Radiation M7
    Biophysics

    Saturday, 10 October 2020


    BLOT: method of transferring proteins, DNA
    or RNA, onto a carrier (for example, a
    nitrocellulose, polyvinylidene fluoride
    (PVDF) or nylon membrane)
    Southern
    Blot
    Southern Blot technique
    1975

    Sir Edwin Mellor Southern


    Northern
    Blot
    Northern Blot technique

    1977, Stanford University

    David Kemp James Alwine George Stark


    Western
    Blot
    Northern
    who invented the Western blot? 1977
    1977 - 1979

    George Stark's group


    (at Stanford)?
    Was it Neal Burnette (at Fred Hutchinson)?

    Was it Harry Towbin's group (in Switzerland)? 1975

    George Stark's group (at Stanford)?

    Southern
    Harry Towbin's group George Stark's group
    Basel, Switzerland (Stanford)

    George Stark,
    Jaime Renart &
    Jakob Reiser,
    published first
    submitting in April
    1979 and published
    in July 1979
    Harry Towbin & Julian Gordon and Theophil
    Staehelin submitted in June 1979 and was
    published in September 1979

    W. Neal Burnette, working in


    Burnette definitely gave the
    Robert Nowinski's lab at the
    technique the name "Western
    Fred Hutchinson Cancer Research
    blotting" as a nod to Southern
    Center in Seattle, submitted in
    blotting and because their lab was
    1979, but it was rejected, then
    on the west coast
    eventually published in 1981
    The term "western blot" was given by W. Neal Burnette
    method was originated in the laboratory of Harry Towbin
    THE BLOTTING
    COMPASS

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