Velasquez, Gwynne Anne C

15-1-83753

1. Laboratory Safety Procedures in Histopathology

- Ensure protective clothing should be worn all the time when conducting experiments.
- Always use safety goggles, mask and gloves to avoid damage when chemicals spill accidentally
- Eyewash area and shower station must be provided at all times
- Proper air ventilation is needed for air regulation specially in different kinds of solution
- Equipment must be decontaminated and regularly tested to see defects.
- Specimen must be received and processed as soon as possible
- Appropriate disinfectant must be used.

2. Fresh Tissue Examination Methods

- Teasing or dissociation – issue is is in isotonic solution and dissected or separated and stained
using different dyes
- Squash Preparation - Small pieces of tissue are placed on a glass slide & forcibly compressed
with another slide (coverglass)
- Smear Preparation - Examination of sediments / Small tissue sections by spreading materials
lightly on slide using applicator.
- Streaking - material directly applied on slide direct or zigzag line
- Spreading - material is transferred on slide, by teasing the mucous strands apart with applicator
stick, to maintain cellular interrelationships.
- Pull Apart – Thick secretion (gastric lavage, serous fluid, blood) are dispersed into two slides
- Touch Preparation - freshly cut tissue is brought in contact & pressed on the surface of the slide,
stained & examined under microscope.
- FINE NEEDLE ASPIRATION - Simple ,safe , rapid cytologic technique using a thin, hollow needle to
examine superficial masses
- FROZEN SECTION - Fresh tissue cut on cold microtome(15-15um), transferred on slide for light
microscopy
- IMPRESSION SMEARS - Used for ulcerated surface lesions. Limited value because they usually
only sample the surface inflammatory exudates and rarely include cells from deeper tissues.