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PhysioEx 1.1 PDF
PhysioEx 1.1 PDF
PhysioEx 1.1 PDF
3. Which of the following dialysis membranes has the largest pore size?
You correctly answered: d. 200 MWCO
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Experiment Results
Predict Question:
Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane?
Your answer : c. No, not at all.
Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average
molecular weight of a single amino acid is 135 g/mole.
Which of the following will be able to diffuse through the 200 MWCO membrane?
Your answer : c. glucose only
Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino
acid is 135 g/mole. There is no reason to run these solutes at the 20 MWCO because
You correctly answered: d. glucose and albumin are both too large to pass.
Experiment Data:
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Post-lab Quiz Results
You scored 75% by answering 3 out of 4 questions correctly.
1. The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to
Your answer: c. no change to the rate of diffusion.
Correct answer: b. increase the rate of diffusion.
2. Describe the difference between the rate of diffusion seen for sodium and urea.
You correctly answered: c. Urea diffused more slowly because it is larger than sodium.
3. Which of the following solutes did not pass through any of the membranes?
You correctly answered: b. albumin
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Review Sheet Results
1. Describe two variables that affect the rate of diffusion.
Your answer:
size of the pores in the membrane and the size of the molecule itself
2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare
with your prediction?
Your answer:
because the molecular weight of each molecule of urea is 3 times bigger than of 20MWCO
3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the
results compare with your prediction?
Your answer:
as for each glucose the total molecular weight would be around 180g per mol and therefore could pass through the
200MWCO while of albumin which has 135g per mol molecular weight for each amino acid which is then composed of 607
amino acid practically can never pass through the 200mwco
4. Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea.
Your answer:
sodium chloride
urea
glucose
albumin
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