PLANT REACTION IN EXPOSURE TO VARIOUS LIQUIDS WHICH AFFECT THEIR

GROWTH
ABSTRACT

This experiment is on plants and how different liquids can affect them. The purpose of this
experiment is to figure out why different liquids affect plants differently. To do the following
experiment you need to 1. Put three kinds of plant in each pot of nine pots 2. Label one pot A, one
pot B & one pot C 3. Water plant A with 1 ½ tbs. of diet drinks 4. Water plant B with 1 ½ tbs. of tap
water 5. Water plant C with 1 ½ tbs. of orange juice 6. Water and measure the plants each day. Too
many nutrients can make a plant die out. Some liquids with nutrients is orange juice. Orange juice
contains protein, which contains nutrients. If too many of these nutrients overlap, they can cause
the plant to die. This is the same with the milk. Sugar can affect plant growth too. Over expressing
Arabidopsis in plants reduces growth in plants. Due to hypertonic states of different liquids, some
juices and sodas can kill plants. This experiment will look at how soda, juice, and water will affect
the growth of plants. Orange juice do not kill plants like regular sodas do. To keep data on how tall
the plants grow and affect the growth of a plant each day we have been putting the heights of each
plant in centimeters on a chart and we count the branches and leaves of the plant. We check the
heights, number of leaves, branches each day and water the plants with their given amount of
liquids in the procedures. The liquids we are using are zero sugar sprite, tap water and orange juice.

INTRODUCTION

It had numerously been stated
that plants need three fundamental elements
to grow, namely: soil, sunlight and water.
However many people do not know that it is
more complex than that. Exact measurements,
right time, and specific type of these elements
are needed to be observed in order to achieve
the maximum growth of a plant. Growing a
plant is intricate, specifically in putting water.
Many people are fond of watering plants with
different liquid substances that they can
dispose. Examples are: the left over orange
juice that the visitors did not finish, the
spoiled milk that had been stuck in the
refrigerator for weeks, the dish and soap
water that had been used for cleaning or
sometimes even urine. Plant physiology are
not really understand by most. They do not
clearly understand the effects of different
water compounds to the growth of their
plants. That is why the focus of this study is to
impart deeper understanding on basic
gardening by studying the correlation of
different liquid substances to a plant growth.
Are plant growth and photosynthesis
impaired following grass rewatering due to
predrought? Journal, 60(13), Retrieved from
http:/jxb.oxfordjournals.org/cgi/content/full
/60/13/3737 doi: 10.1093/jxb / erp216 to
this article, the person claims that their first
attempt to get an orange juice plant to
develop failed. The reason this did not work is
because there is acid and sugar in the orange
juice. Both of these substances can destroy
plants. We chose this topic is because we
want to attempt the experiment but this time
we used “less Acid orange juice variety.
Xiao, W, Sheen, J, & Jang, J.C. (2000). The role
of hexokinase in plant sugar signal
transduction and growth and. Plant Molecular
Biology, 44. Retrieved from
https://www.ncbi.nlm.nih.gov/pubmed/1119
7321. We learned in the introduction to this
article that glucose (sugar) influences plants.
A sugar metabolism has often been related to
the effects of sugar on plants. Nevertheless,
there has been some recent evidence that
sugars can serve as regulatory signals. These
are signals that can regulate the expression of
the various genes involved in many plant life
cycle processes.
Accorrding to the study by Konning, R.
(1997). How do liquids react plants? Retrived
from http://www.bio.net/mm/plant-
ed/1997-December/002800.html. In the
study by R. Konning, I find that if too many
nutrients are placed in one spot of soil then
the plant would die out. This is because it's
hypertonic. When put on the soil, hypertonic
can be caused by Kool aid sugary, salty water
and daily sodas. The explanation for this is if
the plant will start to die out if too much
hypertonic is dissolved in soil. Orange juice
won't cause herbs to die out. KONNER, R. Says
orange juice may not be hypertonic. This may
be real.
Cocoa tree and is officially known as
Theobroma Cacao. Perhaps it is the
temperament of this mother tree that gives
chocolate some strong and exotic flavor.
Cocoa trees flourish only in tropical high-
temperature areas of 20 degrees north and
south of the equator. Cocoa trees are delicate
plants living under tropical forests, and other
tall trees are necessary to block the wind and
sunlight. These small trees have a height of 60
feet in the wild (in most cases they are only
20 to 40 feet in height) but block wind and
sunlight up to 200 feet from hardwood and
other trees. Learn more about raising and
raising cacao trees. Bean pods are offered by
the World Cocoa Foundation. The cacao tree
has large, shining leaves, which is about the
size of the protruding hand. Young trees have
red leaves, mature trees have green. This
gorgeous tree fascinates other plants.Tomato
is a type of plant from the family of
Solanaceae. There are many tomato species tomatoes do.
around the world. It is also very important as
a food for the citizen. This tomato plant is Orange juice has some components

easy to cultivate in area which is in moderate that are beneficial to house or garden plants,

temperature and firstly came from the place such as natural sugars and citric acid, which is

which is in dry west coast of tropical South especially good for acid-loving plants like

America. Tomatoes are commonly called roses, azaleas, and many others. However, it

vegetables, but they are actually fruits. They can also attract bugs with its sweetness, and

are eaten raw or used in cooking. Canned too much acid can damage plants, so it’s best

tomatoes and tomato juice are also popular. used in diluted form, and infrequently.

Tomatoes are grown in all mild regions of the Adding a few spoonful’s to  watering can once

world. They belong to the nightshade family. a day is the best way to use orange juice in

Chilli (Capsicum annuum L. and Capsicum the garden. Sprite No Sugar has the same

frutescens L.) is a hot-tasting tropical berry intense, refreshing lemon-lime taste of

classic Sprite but with no sugar. It's made to
belonging to the Solanaceae family. It was first
quench your thirst and help you keep your
discovered by Christopher Columbus in
cool in any heated moment.
tropical America, and its use spread rapidly
throughout the world because of its pungent
Sprite No Sugar has the same intense,
flavour. Variously classified as herb, fruit or refreshing lemon-lime taste of classic Sprite
vegetable, it is now an inseparable part of but with no sugar. It's made to quench your
thirst and help you keep your cool in any
Asian cuisine. The name “chilli” is derived heated moment.
from the Mexican word, chili. Chilli plants love
heat. They are closely related to
capsicums/bell peppers and also related to
tomatoes (they are in the same family,
Our parents and grandparents keeps
the solanaceae), but chillies prefer their
potted plants on our house's porch. We found
growing conditions a lot hotter. Chilli seeds
their going to be pour in water, sodas, and
need 20°C/68°F to germinate, and it should
even left over juice to "cool" the plants. It did
be 30°C/86°F or more for the fruit to ripen.
not appear to harm them. This made our
Night temperatures should not drop below
group curious and we wondered, "Is it
15°C/60°F, at least not on a regular basis. The
important how you water a plant. Plants do
odd cool spell is ok. Chillies also don't mind
have stimulated growth with an acid-less
humidity as much as sweet peppers or
orange juice, according to an article in Plant
Physiology. In a biology journal, Ross liquids. This experiment will investigate how
Konning from Eastern CT University notes soda, fruit, and water can impact plant
that certain sodas and juices can kill plants growth.
because of the hypertonic states of several

Different liquids contain different additives that could potentially affect the growth of a
plant. This project will reveal how zero sugar, orange juice, and water affect the growth of plants.
Various research has been done that will support the problem of what liquid can stimulate the
growth of a plant more successfully. By measuring each plant after watering them with the three
liquids, we will be able to determine what liquid more positively affects the plants’ growth.

Variables

Independent Variable: the liquids

Dependent Variable: the growth of plants

Controlled Variable: the plant, amount of liquid & amount of light

Controlled Group: water

The hypothesis is that the plant watered with orange juice will grow taller, more leaves
and, branches and has a benefits/good for plants than the plants watered with sodas and water. By
observing the growth and benefits or detriment of 9 potted plants of the three different kind of
plant being watered with 3 different liquids to see which plant grows more and which solution has a
positive on plants. Our group will show that there is a different in the amount each of plant will
grow, and has a positive effects because of what substance is given.
 The purpose of this project is to see if there is a difference in how plants grow and if there
based on what they are watered with. By watering three different plants with three different liquids
and measuring them and observing daily we will prove or disprove our hypothesis. Specifically the
research has the following objectives:

1. Discuss why different liquids affect plants
differently and how different liquids affect
them.
2. Find out what liquid more positively affect
the plants.
3. Find out the reason why other plants not
grow faster and died because of the substance
given.

MATERIALS
Pots (9) Zero sugar sprite
Plants (Thebroma cacao, tomato, Chilli) Table spoon
Centimeter measuring tape or ruler Tap Water
Labels A, B, & C Atomizer spray
Soil Minute maid orange

METHODS

1. Gather all the materials needed 1

5.) Pour the zero sugar sprite Soda, (1 tbs.),
2
2.) Prepare the plants and their respective
Liquids and place them on one designated 1
tap water with salt (1 tbs.), the orange juice
2
location.
1
1 (1 tbs.) to the second plant (chilli plant).
3.) Pour the zero sugar sprite Soda, (1 tbs.), 2
2
4.) After that, immediately put aside the
1 1
tap water (1 tbs.), the orange juice (1 tbs.) second plant and expose it to the sun.
2 2
to the first plant (cacao plant). 5.) Lastly, Pour the zero sugar sprite Soda, (1

4.) After that, immediately put aside the first 1 1

tbs.), tap water with salt (1 tbs.), the
plant and expose it to the sun. 2 2
 1 8.) Observe the plants and examine the
orange juice (1 tbs.) to the third plant
2 changes in those plants. Keep monitoring
(tomato plant). them daily.

6.) In the same manner, put aside the third 9.) Then illustrate and take note of changes in
plant and expose it to the sun. the

7.) When all of those plants are set. Water the plant. Observe how soda, juice and salt can aff

plants daily and measure them. ect plant growth.

Result and Discussion

Table 1. Cacao Plant in different liquid substance (Height)

Cacao plant 1 (zero sugar sprite) Cacao plant 2(tap water) Cacao plant 3 (orange juice)
March 4:170cm March 4:170cm March 4:170cm
March 5:170cm March 5:170cm March 5:170cm
March 7:170 ¼ March 6:170 ¼ March 6: 170 ½
March 7:170 ½ March 7:170 ¼ March 7:170 ¾
March 8:170 ½ March 8:170 ½ March 8:180 cm
March 9:170 ¾ March 9:170 ½ March 9:180 ¼
March 10:170 ¾ March 10: 170 ½ March 10:180 ½

Table 2. Cacao Plant in different liquid substance (Number of leaves)

Cacao plant 1 (zero sugar sprite) Cacao plant 2(tap water) Cacao plant 3 (orange juice)
March 4: 6 March 4:6 March 4:6
March 5:6 March 5:6 March 5:6
March 6:7 March 6:6 March 6:7
March 7:7 March 7:7 March 7:7
March 8:7 March 8:7 March 8:8
March 9:8 March 9:7 March 9:8
March 10:8 March 10:7 March 10:9

Table 3. Cacao Plant in different liquid substance (Number of branches)

 Cacao plant 1 (zero sugar sprite) Cacao plant 2(tap water) Cacao plant 3 (orange juice)
March 4: 1 March 4:1 March 4:1
March 5:1 March 5:1 March 5:1
March 6:1 March 6:1 March 6:2
March 7:2 March 7:1 March 7:2
March 8:2 March 8:1 March 8:2
March 9:2 March 9:2 March 9:2
March 10:2 March 10:2 March 10:3

Table 4. Tomato Plant in different liquid substance (Height)

Tomato plant1 (zerosugar Tomato plant2(tap water) Tomato plant 3 (orange juice)
sprite)
March 4:110 March 4:110 March 4:110cm
March 5:110 March 5:110 March 5:120cm
March 7:120 March 6:120 March 6: 130cm
March 7:130 March 7:120 ½ March 7:140 cm
March 8:130 March 8:130 March 8:150 cm
March 9:140 March 9:130 ½ March 9:160 cm
March 10:140 March 10: 130 ½ March 10:170 cm

Table 5. . Tomato Plant in different liquid substance (Number of leaf)

Tomato plant1 (zerosugar Tomato plant2(tap water) Tomato plant 3 (orange juice)
sprite)
March 4:5 March 4:5 March 4:5
March 5:6 March 5:5 March 5:6
March 7:7 March 6:6 March 6: 8
March 7:8 March 7:6 March 7:9
March 8:8 March 8:6 March 8:9
March 9:9 March 9:7 March 9:10
March 10:9 March 10:7 March 10:11
Table 6. . Tomato Plant in different liquid substance (Number of Branches)

Tomato plant1 (zerosugar Tomato plant2(tap water) Tomato plant 3 (orange juice)
sprite)
March 4:2 March 4:2 March 4:2
March 5:2 March 5:2 March 5:2
March 7:2 March 6:2 March 6: 3
March 7:3 March 7:2 March 7:3
March 8:3 March 8:3 March 8:4
March 9:4 March 9:3 March 9:4
 March 10:4 March 10:3 March 10:5

Table 7. Chili Plant in different liquid substance (Height)

Chili plant1 (zerosugar sprite) Chili plant2(tap water ) Chili plant 3 (orange juice)
March 4:80cm March 4:80cm March 4:80cm
March 5:80cm March 5:80cm March 5:80cm
March 7:80 ¼cm March 6:80cm March 6:80 ½cm
March 7:80 ½cm March 7:80 ¼ March 7:90cm
March 8:80 ½cm March 8:80 ½ March 8:90 ½cm
March 9:80 ¾cm March 9:80 ½ March 9:90 ¾cm
March 10:90cm March 10:80 March 10:100cm

Table 8. Chili Plant in different liquid substance (number of leaf)

Chili plant1 (zerosugar sprite) Chili plant2(tap water) Chili plant 3 (orange juice)
March 4:2 March 4:2 March 4:2
March 5:2 March 5:2 March 5:2
March 7:2 March 7:2 March 7:2
March 7:3 March 7:2 March 7:3
March 8:3 March 8:2 March 8:3
March 9:3 March 9:3 March 9:4
March 10:3 March 10:3 March 10:4

Table 9. Chili Plant in different liquid substance (number of branches)

Chili plant1 (zerosugar sprite) Chili plant2(tap water) Chili plant 3 (orange juice)
March 4:1 March 4:1 March 4:1
March 5:1 March 5:1 March 5:1
March 7:1 March 7:1 March 7:2
March 7:2 March 7:1 March 7:2
March 8:2 March 8:2 March 8:2
March 9:2 March 9:2 March 9:3
March 10:2 March 10:2 March 10:3

Result and Discussion

a. Result

Table 1. The average of all data we collected

Heights leaf Branch

Types of Diet Tap Orange Diet Tap Orange Diet Tap Orange
liquids/Plan soda water juice soda water juice soda water juice
t
Cacao 170.4c 170.3cm 174.6c 7 6.6 7.3 1.6 1.3 1.9
m m
Tomato 125.7c 121.7cm 140cm 7.4 6 8..3 2.9 2.4 3.3
m
Chili 81.7cm 80.2cm 87.4cm 2.6 2.3 2.9 1.6 1.4 2
Average 125.9c 124.1cm 134cm 5.7 5 6.2 2 1.7 2.4
m

b. Discussion
Conclusion:

The tallest plant, more leaves and
branches plant was the plant watered
with Orange Juice. The smallest plant
was the Plant watered with tap water
while the plant watered with zero sugar
soft drinks is the intermediate of the
liquids. This experiment supported our
hypothesis that orange juice would
encourage the growth of the plant.

Appendices
BEFORE AFTER
 References

 Konning, R. (1997).
How do liquids react plants? Retrived from http://www.bio.net/mm/plant-ed/1997-
December/002800.html
 Xiao, W, Sheen, J, & Jang, J.C. (2000). The role of hexokinase in plant sugar signal
transduction and growth and. Plant Molecular Biology, 44. Retrieved from
https://www.ncbi.nlm.nih.gov/pubmed/11197321.