Download as pdf or txt
Download as pdf or txt
You are on page 1of 1

GC Column Selection

for USP Methods


USP Column Classification
Phenomenex
USP Phase Composition Recommendation
G1 Dimethylpolysiloxane oil ZB-1, ZB-1HT Inferno™
G2 Dimethylpolysiloxane gum ZB-1, ZB-1HT Inferno
G3 50 % Phenyl-50 % methylpolysiloxane ZB-50
G5 3-Cyanopropylpolysiloxane BPX70
G7 50 % 3-Cyanopropyl-50 % phenylmethylsilicone 007-225
G14 Polyethylene glycol (average MW 950-1,050) ZB-WAXplus™
G15 Polyethylene glycol (average MW 3,000-3,700) ZB-WAXplus
G16 Polyethylene glycol (average MW 15,000) ZB-WAXplus
G17 75 % Phenyl-25 % methylpolysiloxane ZB-50
G19 25 % Phenyl-25 % cyanopropylmethylsilicone 007-225
G20 Polyethylene glycol (average MW of 380-420) ZB-WAXplus
G25 Polyethylene glycol TPA (Carbowax 20M terephthalic acid) ZB-FFAP
G27 5 % Phenyl-95 % methylpolysiloxane ZB-5MSi,
ZB-5HT Inferno
G27 5 % Phenyl-Arylene-95 % methylpolysiloxane ZB-5ms
G28 25 % Phenyl-75 % methylpolysiloxane ZB-35, ZB-35HT Inferno
G32 20 % Phenylmethyl-80 % dimethylpolysiloxane ZB-35, ZB-35HT Inferno
Polyethylene glycol & diepoxide esterified
G35 ZB-FFAP
with nitroterephthalic acid
G36 1 % Vinyl-5 % phenylmethylpolysiloxane ZB-5MSi,
ZB-5HT Inferno
G38 Phase G1 plus a tailing inhibitor ZB-1, ZB-1HT Inferno
G39 Polyethylene glycol (average MW 1,500) ZB-WAXplus
G41 Phenylmethyldimethylsilicone (10 % phenyl substituted) ZB-5MSi,
ZB-5HT Inferno
G42 35 % Phenyl-65 % dimethylpolysiloxane ZB-35, ZB-35HT Inferno
G43 6 % Cyanopropylphenyl-94 % dimethylpolysiloxane ZB-624
G46 14 % Cyanopropylphenyl-86 % methylpolysiloxane ZB-1701P

Our Technical Support and Resources are Available to You!


1

phy
atogra
NS Chrom

ICATIO
Gas
ity in
038 nsitiv
TN-2
APPL
Hig her Se
e and
is the
ture
ap sing tempera point is -
50
ak Sh Focu
Solventthe initial
oven
starting This tem
er Pe cting good yte. time
Sharp
,
rs Affe focusing adjust. A eluting anal less hold
Attain
mete ent er to earliest
GC Paradoing solv paramet the split column.
ys to t When enient ng point the dura
of the tion of onto the
is a col-
2 Wa r to mee
in orde solvent
conv
most w the boilibe held for ple is focu
sed
sing ratio
ent focu phase
ysts
can do . 1) The ificing TN-203
ºC belo should entire sam
ture the
ts solv ture, the The lowe
r

APP6LIC
anal ents out sacr get pera that affec pera mn. unt
irem that
s that requ y with order to re er l tem colu the amo se.
le thing s (MDLs) sensitivit al in to ensu parametke the initia a different greater pha
simp limit ter is critic nd GC Unli installing ), the ionary sing
are twodetection for greaselection seco se ratio. ness in the stat ent focu

ATION
e s The by thick solv
Ther od e allow line. pha chan ged
mn base umn’s film dissolve allow
the meth techniqu er GC colu lowest low be (greaterthat can may le.
focusing n. 2) Prop e with the can onlyse ratio column sample

S
very viab
require abuse n
or analyte se ratio were not is the
resolutio peak shap the phaent and ted.
the best (GC)
analyses s of ofte Improv of solv with a that prev
low pha iously sing
ent focu be man
ipula
with
phy as drug analysts to meet ed Sep Working s ts solv cannot achieved ent’s
ction matogra ytes,
such
ever , GC enough GC Co aration tem perature affec
er that the sam sing is best
ple
the solv if
Intr odu
Gas
Chro et anal . How is sens itive lumns at
of Blo met
GC paramost casesolvent focu
s,
betw
een example,
Many of targ aminantshod that od rence ture. For point. 77.1-
detection ental
cont a met attaining The third ents. In possible, test diffe Alcoho tempera (boiling (boil
ining
environm with attaction limit
s (MD
Ls).
le way
s of achieve compon , when the greal column l acetate loromethane ls Using
simp that you can is a ever
How ent that has the initia ºC, ethy than dich Zebron
struggle hod dete s two so sing, less 30 t ™
itivity solvent focu a solv point and ture is sing effec ZB-BA
the met discusse sens n or
split is
The , ng pera e1
l note high er , olum
strategying on-c mn sele ly pest ction Zebr boili
on ZB-B initial tem
a grea
ter focu In Phas C1 and
technica shape and first mak GC colu shar reproducib the
AC1 and ed run. 3 the oven
BAC2
This s. The when line and equa provide le and will have ramm
re progPhases 2
and
peak er te sepa ºC)quan 39 ºC).
tZB-B
sharper ired MDL by anal ysts
tegy , prop st base will helpin unde ents . ration ing pointitativ
AC2 GC
colum temp
eratu
re. In
s a
the requ e used nd strathat the
lowe irem
methodMDL requtosam r 2 minutes ethan
of e analy during ns
ol1.andibution sis of initial temp
eratu
provide orate
.
techniqu s. The
seco re these pler provi Figure distr
with other low the blood evap
nt to alcohextreme-
tion to ensu both of s with low des cons a ~1 set
Analyte
at a blood
injec order zing Introductout –
istent is 3 %ing the solve alcoh ols. Ad-
al in ined. Utili GC method with ion
Thesdete the the oven injeccaus RSD. The ols is Quan
tifica
critic
is atta lts for ow peak
s up tion volum Hta HT-2achieved and aceto tion of
peak resu get narrwithout mostsplittingrmin -ation ramp
es. 00H au- ne is methanol,
is to com y with of bloo Typical
the best s cal itivit moners, tests d inter usually ethanol,
g focusing ow peak ter senspara BACmet perfo alcoh butanol. nal stand done using isopr
Focusinsolvent ining narr s for grea prev GC ent cont
analyricalsis invol rmed ol content nal stand Many foren ards inclu an interopanol, aceta
Solvent ose of tion. Atta allow ing thevolat symmet
and ile com amination
ves headby forensic (BAC) is for using ards depe sic scientistsde t-butanol,nal standard ldehyde, 1
purp use it ectly sett space-ga laboratori one of the
The a split injec t beca narrowbegun ponents at the more nding will use n-pro method.
rtan By corr obtain of etha usingmec ha- in the head of the s chromatoes. A typi-
been than one on their pano
doing is impo lution. to dual- samp
show
interfere n to be spec any of these l, and 2-
sample ificing resocan be usedyses. ensu nol.
firstThes ple colum
n GC le. Man GC colum graphy to present internal stand ific needs. three
. The
re rized sam e two analyses y place n from ally be with quan Another inter-
out sacr focusing rity of anal differentthat
sms any gas is columns s have non- precise titati in postmort ard is that
mechani n a vapo of a analy to
s, ittes ident must have confirm the recently to withi ve results. em spec n-pro reaso
n
solvent for the majo two whe volume reten tion The goal imens, pano
s rs via tions the n ple cond time
ense ified differ pres n±5
%.
Quan
titative which l has
peak occu less injec ause Whe performin mecha-on the seco on the first ent selectivitieence new GC of this stud results would
sing split Bec findinsam nd re- nd colum phas y
focu ng mn. n the The g two seco sg and dual colum n elute s to ident es, Zebr is to dem must
Solvent rs duri l colu d, whe all analy dissi
tion colum n. sing.ify
cohol and quan
usu-
occu on a coo of a liqui the column. injec milar both
nes n analysis,
at a solvent focu on™
interferen tify trace ZB-BAC1 rate the capa
onst
nism s analy n tes,
olum mec hanism, phas
while t sing
ense
cond larger than small area
that on on-c sis,
and fastthe firstbaseline colu mn upo
main tainin
that prov the majo rs that affec ent minu
Focu
tes). ts in amo and ZB-B bilities
the ide 1. challenge t on Solv blood
r mete All samp blood unts AC2, of
a less ke analyon the ” on
resol g
utionas reasonable Tableadeqmuate les were with fast of alcohols to accu two
much sed into both split ram. Unlihighense sis timezone para Affec alcoh
syste resolution is etaldehyd ol analy or poss rately
is focu lies to oven prog must conds a “floo
throughpu ded (und s in size for all
analyGC
analy rs analyzed analysis
app ytes formmostly decrease t natu er arefivedis-minu tes Paramete
sis times of 0.050, e, etha tes in each by head times (from ible al-
nism a ramped
the anal d samplethereslow
The re of
that most has tes) areGC of interest . In BAC 0.100, nol, aceto samp
le cons space-GC 2 to 4
quires ent and ense ed zone are theotheanalytes
critic
the solv
al bloo
ent critic
theforensic labs. al beca and ultra
is in acco 0.20 ne,
rdance 0, and 0.40 and isopr isted of meth-FID. The
all of t ban d on
d alcoh use of a DUI
the solv . The cond The flood entr strea
atingm time racom
that tighpouneitherolthe the from
conviction with the 0 % in opanol
dilute anol, ac-
tion ace. conc of the may com poun typic wate
injec surf orates, 1). Byethanol. into inter r, ds that onto could d is ethanol.
the in
detection chromato all 50 state al 0.08 % This calibratioto 0.025,
r. d
column’sent evap ent (Figure in focused sing
beetha First,to occu fere ense grams blood
nol a t cond with the be pres five analy (LOD) and s in the- n rang
the solvin the solvanalytes
willsmal focuas a bypr mussmal l amo identificat ent in However, have chro US. Sign alcoh
ol limit e
solventl amo ytes oduct unt of a the bloo tes. a limit gasbeenron
solved ed, the s ofof etha the analunt of of the t meth ion or
d HP6890of quan Zeb used to deteal-to-nois for
orat form
ent and nol consump anolwhic
meth h affecdistillationanol is usuaquantitati The two on a enex al titati pa- on rmine e data
evap For boththe solv ent of rubb em may meters on ethanol newucted a Phenom ition (LOQ) a
column. or both of abso ingGC systtion. Seco
also
e para solv beent process. lly pres
ent cond GC
was from g columμm. Add for each limit of
in an throu
rptio
alcoh
ol) Thes of the nd, isoprpres ent Therefore ntal sample nolo
abilit ) usin close
to quanID x 0.50
y gies
n phas
ly elutinre 2.es prov of the
analytes mn. vapoers
met r. Once(Tab le 1). can tilityalso lbenote , weopan in bloo d asrime ,a – 0.40 tify in Figu g com
gh skin ent ol (the main Expe a resulof eachlent Tech 0 %.mm ided
the colu ral para oxidi ze tion in thethebloo vola
techor nica tpres
from solvent in the Analysis t(Agi m xto0.53
tive Rela deda bloo pounds enhanced
ensa ture,
to aceta ingreph mn, 30 are inclutive stand d alcohol while resol
are seve of cond when pera In this d strea
. ldehy
affec inhal
l oven blood
as aogra di-
method
the
ard devia concentra also exhib ution of
There rate l oven temtestinratio de.met ers m,
thethe initia ation
lts of mat GCt colu
resul
and 0.10 internal
prom se g for
inent GCBAC para Anot
gingher meta
cons
resu umed rubbing 35 the GCapplicatio0 % conc standards tions tion
(RSDe 1 of 4between
iting the
sample’sthe initia of theketo pha in diab is aceto
chan phic etha ZB- alcoh s for entration , have
de different how
rate etics chro
ogra
ne. This bolite to take nol may rameterol n. beenPag ), absolute 0.02
inclu and
how acido becamat levels
to illust tabulated and rela-5
analyte,ussing Since dem
onst sis. oved use aceto meta bolite into account
also Experime
rate the at the
and disc also manyide impr ne is is parti All samp ntal
will be . We willnol produced precision 0.025
easi onlyaprov of the
mentioned cular to 0.02 les analy
focusing ture can sibleshape. GC as a resully zed of the
inter column, com (total 5, 0.050, 0.10 consisted
PO72700110_L

content. ferences most BAC pounds t


tempera per peak may centratio
volum
e) insid 0, 0.20 of bloo
with
shar portant To ensure into account analyses need co-elute 0.100 n of inter e a 20 mL0, and 0.40 alcohol analy
d
ethanol to use GC proper ident when dete to take with etha- %. nal stand headspac 0 % in tes
colum rmining these gas chro Analysis 5.0 mL diluted
from all
other ns that ification and bloo pos- tosam matograp
of each ards in
each
e vial.
Note of wate
compone canenex prov.com quantitati d alcohol pler and h (Agile sample was samp that
le was the con-
r
.phentsnompote ide the on, it Zebron two nt) equip cond
visit www
ntial interbest resol is im- μm and ZB-BAC1 new capillary ped with ucted on
always
notes, ferences. ution of had dime an an HP68 at
nical ID x 2.00the Zebron nsion
colum
ns from Hta (HT-2 90
l tech lead from μm. The ZB-BAC2 s of Phen 00H)
addi tiona
the colum had dime30 m x 0.53 omenex. au-
For into two same ns were nsion mm ID The
eters sepa injec insta s of 30 x
for the rate flame tion port and lled such m x 0.53 3.00
autosamp ionization guar that they mm
ler and dete d colum woul
GC meth ctors. Addi n and split d
od are tional off
listed para
in Table m-
1.

For addit
ional techn
ical notes
, visit
www.phen
omen
ex.com

Knowledgeable
Page
1 of 4

FREE FREE FREE

Technical Notes Troubleshooting Guide Users Guide GC Specialists


www.phenomenex.comnx
Phenomenex products are available worldwide. Email us at info@phenomenex.com.
© 2010 Phenomenex, Inc. All rights reserved.

You might also like