B.

Sc Hons 2nd Semester Examination, 2020

Sub: Microbiology, Unit: CC3
Full marks: 25
Time: 1hour

Group A is compulsory and answer any three from Group B

Group A
Multiple choice questions (Select the correct answer) For ten questions 10 x 1 = 10
1. Oxido reductase enzyme catalyses
a. The transfer of electron
b. The transfer of proton
c. The transfer of water molecule
d. The transfer of hydroxyl ion
2. The unit of Vmax is
a. Micro mole / min
b. Micromolar
c. Micromole
d. Millimolar
3. The nature of Michaelis-Menten curve is
a. Sigmoidal
b. Hyperbolic
c. Z-curve
d. Straight line
4. The pH activity profile of enzyme is
a. bell shaped curve
b. Straight line curve
c. Sigmoidal curve
d. Zigzag line curve
5. Purine base contains
a. Two six member rings
b. One five member ring
c. One six member and one five member ring
d. One six member ring
6. Adenine is
a. Purine base
b. Pyrimidine base
c. Nucleotide
d. Nucleoside
7. In adenosine, Adenine is connected to ribose by
a. Glycosidic bond
b. Phosphodester bond
c. Hydrogen Bond
d. Covalent Bond
8. In di nucleotide the connecting bond occurs between
a. 2’OH group of 1st nucleotide and 5’phosphate of 2nd nucleotide
b. 3’ OH group of 1st nucleotide and 5’ phosphate group of 2nd nucleotide
c. 2’OH group of 1st nucleotide and 4th carbon of 2nd nucleotide
d. 1’ OH group of 1st nucleotide and 5’ phosphate of 2nd nucleotide
9. In double stranded DNA base pairing of between complementary strands occurs via
a. Phosphodiester bond
b. Hydrogen bond
c. Glycosidic bond
d. Covalent bond
10. In Watson crick model double stranded DNA
a. Adenine pairs with Thymine
b. Adenine pairs with Guanine
c. Adenine pairs with cytosine
d. Adenine pairs with adenine
 Group B
Answer any Three ( 3x5 = 15)

11.a..Define Km and also mention its Unit. 1.5

b.How would you determine the value of km and Vmax if the values of substrate concentrations and
velocity of the enzyme catalyzed reaction are available ? 3.5
12. a. Compare competitive inhibition and non competitive inhibition. 3.5
b. Define Kcat and also mention its unit. 1.5

13. a. Define activity and specific activity. How specific activity is related with purity of enzyme. (1+1) + 1
b. Compare allozyme and isozyme. 2
14. a. Explain feedback inhibition with suitable example. 2.5
b. Compare homotropic and heterotropic enzyme. 2.5
15. a. Explain irreversible inhibition. 3
b. Give an example of suicide inhibition. 1
c. What is ribozyme? 1
16. State the 1st law and 2nd law of thermo dyanamics. 2.5 + 2.5