A spectrophotometric method of Lipid Profile was done in a clinical laboratory.

The laboratory
used Lipid Profile reagent kit that contains a bottle of standard with the following conc:
(Triglycerides=130 mg/dL, Cholesterol= 100mg/dL, HDL=40mg/dL).

Following the usual protocol, COMPUTE FOR LIPID PROFILE (Total Cholesterol, Triglycerides,
HDL, LDL).

GROUP 8
Results of spectrophotometric reading are as follows:

TRIGLYCERIDES CHOLESTEROL HDL

BLANK 0.029 0.031 0.032

STANDARD 0.589 0.590 0.591

CONTROL NORMAL 0.513 0.510 0.515

CONTROL PATHOLOGIC 1.079 1.076 1.080

PATIENT NAME:
JO JUNG-SUK (M/ 42 YEARS OLD)

TRIGLYCERIDES 0.605

CHOLESTEROL 0.936

HDL 0.680
 Exercise 10.1 - cholesterol
1. Label the following reaction tubes and indicate the color reaction observed in each
component of the analytical run.

2. COMPUTATION and EVALUATION OF RESULTS: Compute for the values of CN, CP,
and U. And evaluate the results by answering the following questions:

ANALYTE REFERENCE RANGE

CHOLESTEROL < 200 mg/dL < 5.17 mmol/L

EXPECTED RANGES OF CONTROLS

Elitrol I 62.9 – 85.3 mg/dL

(normal control)
1.63 – 2.21 mmol/L

Elitrol II (pathologic 156.4 – 211.2 mg/dL

control)
4.05 – 5.47 mmol/L

Conversion factor: mg/dL x 0.0259 = mmol/L

EXPECTED RANGES OF CONTROLS

Elitrol I 62.9 – 85.3 mg/dL

(normal control)
1.63 – 2.21 mmol/L

Elitrol II (pathologic 156.4 – 211.2 mg/dL

control)
4.05 – 5.47 mmol/L
 Value Expected Range Interpretation

CN 86.4 mg/dL 62.9 – 85.3 mg/dL

REJECTED
2.2 mmol/L 1.6 – 2.2 mmol/L

CP 182.4 mg/dL 156.4 – 211.2 mg/dL

ACCEPTED
4.7 mmol/L 4.0 – 5.5 mmol/L

Value Reference Range Interpretation

JO 158.6 mg/dL <200 mg/dL ACCEPTED

JUNG-SUK
4.1 ​mmol/L < 5.17 mmol/L

0.510
C oncentration (CN ) = 0.590 × 100 mg/dL = 86.4 mg/dL
* 1 decimal points to f ollow sig f ig of ranges
1.076
C oncentration (CP ) = 0.590 × 100 mg/dL = 182.4 mg/dL
0.936
C oncentration (U ) = 0.590 × 100 mg/dL = 158.6 mg/dL

a. Is the test valid? Explain your answer. Can you release the result?
The test is invalid. The patient’s cholesterol level is within the reference range but
the obtained values for normal control are not within the expected
range.Therefore, the result cannot be released.

b. What can you say about the obtained cholesterol values of the patient? Are
they within the reference range? Correlate your answer with the clinical
significance.
The obtained cholesterol values of Mr. Jo Jung-Suk are within the reference
range, which means his cholesterol level is normal. However, the test results
cannot be released since the test is invalid.
 3. Result Form Clinical Chemistry Laboratory

IF MINUS SA BLANK:

Value Expected Range Interpretation

CN 85.68 mg/dL 62.9 – 85.3 mg/dL

REJECTED
2.22 mmol/L 1.6 – 2.2 mmol/L

CP 186.94 mg/dL 156.4 – 211.2 mg/dL

ACCEPTED
4.84 mmol/L 4.0 – 5.5 mmol/L
 Value Reference Range Interpretation

JO 161.90 mg/dL <200 mg/dL NORMAL

JUNG-SUK
4.19 mmol/L < 5.17 mmol/L

0.510−0.031
C oncentration (CN ) = 0.590−0.031 × 100 mg/dL = 85.68 mg/dL
* 1 decimal points to f ollow sig f ig of ranges
1.076−0.031
C oncentration (CP ) = 0.590−0.031 × 100 mg/dL = 186.94 mg/dL
0.936−0.031
C oncentration (U ) = 0.590−0.031 × 100 mg/dL = 161.90 mg/dL

Exercise 10.2 - HDL

1. COMPUTATION and EVALUATION OF RESULTS: Compute for the values of CN,
CP, and U. And evaluate the results by answering the following questions:

ANALYTE REFERENCE RANGE

HDL 40.0 ​– ​75.0 mg/dL 1.0 ​– ​2.0 mmol/L

EXPECTED RANGES OF CONTROLS

Elitrol I 26.5 – 35.8 mg/dL

(normal control)
0.7 – 0.9 mmol/L

Elitrol II (pathologic 61.2 – 82.8 mg/dL

 control) 1.6 – 2.1 mmol/L

Conversion factor: mg/dL x 0.0259 = mmol/L

Value Expected Range Interpretation

CN 34.6 mg/dL 26.5 – 35.8 mg/dL

ACCEPTED
0.9 mmol/L 0.7 – 0.9 mmol/L

CP 75.0 mg/dL 61.2 – 82.8 mg/dL

ACCEPTED
1.9 mmol/L 1.6 – 2.1 mmol/L

Value Reference Range Interpretation

JO 46.4 mg/dL 40.0 - 75.0 mg/dL

JUNG-SUK NORMAL
1.2 mmol/L 1.0 - 2.0 mmol/L

0.515−0.032
C oncentration (CN ) = 0.591−0.032
× 40 mg/dL = 34.6 mg/dL
* 1 decimal points to f ollow sig f ig of ranges
C oncentration (CP ) = 1.080−0.032 0.591−0.032
× 40 mg/dL = 75.0 mg/dL
C oncentration (U ) = 0.680−0.0320.591−0.032
× 40 mg/dL = 46.4 mg/dL
a. Is the test valid? Explain your answer. Can you release the result?
The test is valid since both given controls are within the expected ranges
of control. The results can therefore be released.

b. What can you say about the obtained HDL cholesterol values of the
patient? Are they within the reference range? Correlate your answer with
the clinical significance.
The obtained HDL cholesterol values of the patient is within the reference
range. This means that the patient is normal and healthy

2. Label the following reaction tubes and indicate the color reaction observed in each
component of the analytical run.
3. Result Form Clinical Chemistry Laboratory
 Exercise 10.3 - triglycerides
1. COMPUTATION and EVALUATION OF RESULTS: Compute for the values of CN,
CP, and U. And evaluate the results by answering the following questions:
 ANALYTE REFERENCE RANGE

Triglycerides 60.00 - 150.00 mg/dL 0.10 - 1.70 mmol/L

EXPECTED RANGES OF CONTROLS

Elitrol I 93.00 – 129.00 mg/dL

(normal control)
1.05 – 1.46 mmol/L

Elitrol II (pathologic 187.00 - 259.00 mg/dL

control)
2.11 - 2.93 mmol/L

0.513−0.029
C oncentration (CN ) = 0.589−0.029
× 130 mg/dL = 112.36 mg/dL
* two decimal points to f ollow sig f ig of ranges
C oncentration (CP ) = 1.079−0.029 0.589−0.029
× 130 mg/dL = 243.75 mg/dL
0.605−0.029
C oncentration (U ) = 0.589−0.029 × 130 mg/dL = 133.71 mg/dL

Value Expected Range Interpretation

CN 112.36 mg/dL 93.00 – 129.00 mg/dL ACCEPTED

1.27 mmol/L 1.05 – 1.46 mmol/L

CP 243.75 mg/dL 187.00 - 259.00 mg/dL ACCEPTED

2.75 mmol/L 2.11 - 2.93 mmol/L

Value Reference Range Interpretation

JO 133.71 mg/dL 60.00 - 150.00 mg/dL NORMAL

JUNG-SUK
1.28 mmol/L 0.10 - 1.70 mmol/L

a. Is the test valid? Explain your answer. Can you release the result?
 The test is valid since both given controls are within the expected ranges of
control. The results can therefore be released.
b. What can you say about the obtained Triglyceride values of the patient?
Are they within the reference range? Correlate your answer with the clinical
significance.
The obtained triglyceride values for Mr. Jo Jung-suk is seen to be within the
range.

2. Label the following reaction tubes and indicate the color reaction observed in
each component of the analytical run.

3. Result Form Clinical Chemistry Laboratory