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6.3 Enzyme Kinetics Lab
6.3 Enzyme Kinetics Lab
The rate of reaction of an enzyme is affected by many factors such as temperature, and pH.
But as you’ll see in this lab activity, the reaction rate of an enzyme can also be affected by the
concentration of substrate, competitive inhibition, and noncompetitive inhibition.
Materials:
100 colored coins (pennies)
10 uncolored coins (nickels)
Stopwatch
Adhesive tape
Cloth blindfold
Analysis:
1. How does substrate concentration (number of pennies) affect the enzyme activity
(number collected)?
2. Around 50-70 coins the number of substrate molecules that the enzyme is able to
react with (collect) seems to level off. Why? What is happening?
Analysis:
3. How do competitive inhibitors (nickels) effect the reactivity of your enzyme with the
correct substrate (pennies collected)?
4. At what point does your enzyme reach saturation? (At what point does the number of
pennies collect seem to level off?) Does this saturation point occur at the some point as
in activity one?
1. To simulate a noncompetitive inhibitor, tape together the middle three fingers of your
enzyme.
2. Repeat the activity as in part 1 without the competitive inhibitors (nickels) from part 2.
3. Record you data in the table below. Record the number of colored coins that your enzyme is
able to collect for each 30 time period by creating a data table containing the independent and
dependent variables.
Analysis:
5. What happens to the enzymes ability to react with the substrate (collect pennies)
when a noncompetitive inhibitor is bound to it?