REVISED GUIDELINES FOR DRY LAB BIO150

Introduction to Lab Session of BIO150:

The lab activities of BIO150 are designed to complement with the syllabus so that student
could be exposed to biochemical technique and to apply the concepts and theories that have
been learned.

A full detailed written step-by-step lab instruction and methodology may not be provided.
This is to encourage student to participate in active learning during class and to correlate all
the theories and concepts presented in lectures.

The BIO150 lab session might challenge student at early stages in learning a complex skill
that includes initiation, trial and error.

Students are required to produce a written scientific lab report based on scientific
experiments. This specific task is related to the practical skills that requiring student to
perform (plan, conduct and analyse) scientific investigations in areas of biochemical
processes and cell division.

During Lab Video Simulation Session:

1) You should be conscious, focus and have a lab notebook to jot down every act in the
video simulation.
2) Ask question if you have any, regarding the lab demonstration shown during
simulation video (use any medium suitable for you and your lecturer for discussion
e.g: Telegram/ UFUTURE/ Whattsap / ilearn/ google classroom/ teams).

After Lab Video Simulation Session:

1) Select only one simulation video. You would have to submit a written scientific
laboratory report on week 9 (December 14, 2020 until December 18, 2020). Please
refer to the laboratory report checklist and rubric for formatting and contents.
2) You are required to include with precise information as following table below in your
scientific laboratory report:

No. Contents Writing Guidelines/ Required Information

1 Introduction  A short introduction that provide a background of the
topics.
 State clearly about the objective, problem statement
and hypothesis of the experiment.
2 Apparatus &  List down all the apparatus, materials, samples,
Materials reagents or chemical, etc. Please be a detailed as
possible (e.g: amount, concentration etc).
3 Procedure  Provide a schematic diagram of the workflow of the
experiment.
 List down the entire step as complete as possible in
such that anyone can repeat the experiment.

1
4 Results  Provide a suitable figure/table/caption/datasheet for
the experiment.
 Acknowledge and put a reference if the figure is taken
from other sources.
5 Discussion  Discuss your result or technique used (if any) in the
experiment.
 Correlate the findings with any suitable concept and
theories that you have learned in class.
 Provide possible source of error and future
recommendation.
6 Conclusion  Provide a concise conclusion on the experiment.
 State whether hypothesis of experiment was
acceptable or not. Conclude based on objective.
7 References  Provide a list of reference according to APA style that
has been used in your lab report.

2
 Video Simulation 01: ENZYME I -THE FASTEST ENZYME: CATALASE

A) Instruction:

1. Watch the simulation video of enzyme I - the fastest enzyme: catalase.

The catalase enzyme breaks down hydrogen peroxide (H2O2) into water and oxygen.
2H2O2 -----catalase----→ 2H2O + O2

2. Write a lab report according to the given format.

B) Lab Learning Objectives:

1. Identify the factors influence the reactions of catalase enzyme.

2. Analyse the reaction of catalase on different samples.

C) Apparatus and methods:

1. Use this link to watch the simulation video on enzyme I - the fastest enzyme:
catalase: https://youtu.be/yeYbCMWrEJI

2. You need to identify and refer any articles/websites related to the experiment to write
a scientific report from the simulation video.

3. Use table (Appendix I) to record the result based on the simulation video.

3
 Video Simulation 02: CELLULAR RESPIRATION

A) Instruction:

1. Watch the simulation video of cellular respiration of yeast.

2. Write a lab report according to the given format.

B) Lab Learning Objectives:

1. Identify the type of cell respiration in the yeast.

2. Interpret the results of yeast reaction by using methylene blue as the artificial
hydrogen acceptor.

C) Apparatus and methods:

1. Use this link to watch the simulation video on cellular respiration of the yeast:
https://youtu.be/wwy_Uc7sdlg

2. You need to identify and refer any articles/websites related to the experiment to write
a scientific report from the simulation video.

3. Use table (APPENDIX II) to record the result based on the simulation video.

4
 Video Simulation 03: PIGMENT COMPOSITION OF CHLOROPHYLL

A) Instruction:

1. Watch the simulation video of pigment composition of chlorophyll.

2. Write a lab report according to the given format.

B) Lab Learning Objectives:

1. Prepare a chlorophyll extraction.

2. Identify the type of pigments in the leaves by using paper chromatography technique.

3. Calculate the Rf value each of the pigments.

C) Apparatus and methods:

1. Use this link to watch the simulation video of pigment composition of chlorophyll:
https://youtu.be/5t-LQlOJCCg

2. You need to identify and refer any articles/websites related to the experiment to write
a scientific report from the simulation video.

3. Analysis of results:

The pigments migrate a different distance on the chromatography paper. However,

the migration of each pigment relative to the migration of the solvent would not
change. This migration of pigment relative to migration of solvent is expressed as a
constant, Rf (Reference front). It can be calculated by using the formula:

Rf = Distance pigment migrated (x)

Distance solvent front migrated (y)

4. Use table (APPENDIX III) to record the result.

5
 APPENDIX I

Table 1: Catalase reaction

Test tube containing Observations Presence of Oxygen

Cube of liver

Ground liver

Boiled liver

Cube of kidney

Ground kidney

Cube of potato

Ground potato

6
 APPENDIX II

Table 2: Yeast reaction

Colour Observed
Treatment
Test tube A Test tube B Test tube C

Drops of
methylene blue
and shaking the
test tubes

First incubation -
After 15 minutes

Vigorous shaking

Second
incubation - After
15 minutes

7
 APPENDIX III

Table 3: The Rf value and types of pigments in leaves

Pigments Colour Rf

Carotene

Xantophyll

Chlorophyll a

Chlorophyll b