HOST INTERFACE SPECIFICATION

CELL-DYN® 1200 SYSTEM

LIST NO. 03H19-01

Revision B

ABBOTT DIAGNOSTICS DIVISION

© 2000 Abbott Laboratories
Abbott Park, IL 60064
 Table of Contents

1 INTRODUCTION............................................................................................1
1.1 Scope .......................................................................................................................................................... 1

1.2 Definitions and Conventions.................................................................................................................... 1

2 MECHANICAL INTERFACE .......................................................................1

2.1 Connector Arrangement .......................................................................................................................... 1

3 ELECTRICAL INTERFACE.........................................................................2

4 DATA INTERFACE........................................................................................2

5 COMMUNICATION PROTOCOL ...............................................................2

5.1 Communication Modes ............................................................................................................................ 2
5.1.1 Run Menu Automatic Transmit Mode................................................................................................... 2

5.2 Transmission Control............................................................................................................................... 2

5.3 Response from Host.................................................................................................................................. 3

5.3.1 Between-Transmission Time-Out.......................................................................................................... 3
5.3.2 Release for Next Message...................................................................................................................... 3
5.3.3 Retransmission....................................................................................................................................... 3

5.4 Message Format........................................................................................................................................ 3

5.4.8 Message Length ..................................................................................................................................... 3
5.4.9 Data Representation............................................................................................................................... 4

6 IDENTIFICATION (ID) SEGMENT ............................................................4

6.1 Identification ............................................................................................................................................. 4

6.2 Message Type - Field 1 ............................................................................................................................. 4

6.3 Instrument Type - Field 2 ........................................................................................................................ 4

6.4 Serial Number - Field 3 ............................................................................................................................ 4

6.5 Sequence Number - Field 4 ...................................................................................................................... 5

6.6 Specimen Type - Field 5 ........................................................................................................................... 5

6.7 Operator ID - Field 6................................................................................................................................ 5

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 6.8 Specimen Date - Field 7............................................................................................................................ 5

6.9 Specimen Time - Field 8........................................................................................................................... 5

6.10 Specimen ID - Field 9 ............................................................................................................................... 5

6.11 Spare Field - Field 10................................................................................................................................ 6

6.12 Spare Field - Field 11................................................................................................................................ 6

6.13 Spare Field - Field 12................................................................................................................................ 6

6.14 Spare Field - Field 13................................................................................................................................ 6

6.15 Spare Field - Field 14................................................................................................................................ 6

6.16 Spare Field - Field 15................................................................................................................................ 6

6.17 Spare Field - Field 16................................................................................................................................ 6

7 RESULTS SEGMENT ....................................................................................6

7.1 Results Format.......................................................................................................................................... 6

7.2 Histogram Messages ................................................................................................................................. 6

7.2.1 Scale Factor - Field 17 ........................................................................................................................... 6
7.2.2 Channel Data - Fields 18 through 273 ................................................................................................... 7

7.3 Count Data Message................................................................................................................................. 7

7.3.1 WBC Count - Field 17 ........................................................................................................................... 7
7.3.2 Spare Field - Field 18 ............................................................................................................................ 7
7.3.3 LYM Count - Field 19 ........................................................................................................................... 7
7.3.4 MID Count - Field 20 ............................................................................................................................ 8
7.3.5 GRAN Count - Field 21......................................................................................................................... 8
7.3.6 Spare Field - Field 22 ............................................................................................................................ 8
7.3.7 RBC Count - Field 23 ............................................................................................................................ 8
7.3.8 HGB Value - Field 24............................................................................................................................ 8
7.3.9 HCT Value - Field 25 ............................................................................................................................ 8
7.3.10 MCV Value - Field 26 ...................................................................................................................... 9
7.3.11 MCH Value - Field 27 ...................................................................................................................... 9
7.3.12 MCHC Value - Field 28.................................................................................................................... 9
7.3.13 RDW Value - Field 29 ...................................................................................................................... 9
7.3.14 PLT Count - Field 30 ........................................................................................................................ 9
7.3.15 MPV Value - Field 31....................................................................................................................... 9
7.3.16 PCT Value - Field 32 ...................................................................................................................... 10
7.3.17 PDW Value - Field 33..................................................................................................................... 10
7.3.18 Spare Field - Field 34...................................................................................................................... 10
7.3.19 LYM % Value - Field 35 ................................................................................................................ 10
7.3.20 MID % Value - Field 36 ................................................................................................................. 10
7.3.21 GRAN % Value - Field 37.............................................................................................................. 10
7.3.22 Spare Field - Field 38...................................................................................................................... 10
7.3.23 Moving Average Flag - Field 39..................................................................................................... 10
7.3.24 Spare Flag - Field 40....................................................................................................................... 10
7.3.25 R4 WBC Flag - Field 41 ................................................................................................................. 11

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 7.3.26 GR3 WBC Flag - Field 42 .............................................................................................................. 11
7.3.27 MR3 WBC Flag - Field 43.............................................................................................................. 11
7.3.28 MR2 WBC Flag - Field 44.............................................................................................................. 11
7.3.29 LR2 WBC Flag - Field 45............................................................................................................... 11
7.3.30 R1 WBC Flag - Field 46 ................................................................................................................. 11
7.3.31 R0 WBC Flag - Field 47 ................................................................................................................. 11
7.3.32 Spare Flag - Field 48....................................................................................................................... 11
7.3.33 Status Flags - Fields 49 - 54............................................................................................................ 11
7.3.34 Spare Flags - Fields 55 through 60 ................................................................................................. 12
7.3.35 LRI Flag - Field 61 ......................................................................................................................... 12
7.3.36 URI Flag - Field 62 ......................................................................................................................... 12
7.3.37 WBC Count Time - Field 63........................................................................................................... 12
7.3.38 Spare Field - Field 64...................................................................................................................... 12
7.3.39 RBC Count Time - Field 65............................................................................................................ 12
7.3.40 WBC Flow Error Code - Field 66................................................................................................... 12

Flow Errors .............................................................................................................................................................. 13

7.3.41 RBC Flow Error Code - Field 67 .................................................................................................... 13
7.3.42 Spare Field - Field 68...................................................................................................................... 13
7.3.43 Limits Set - Field 69 ....................................................................................................................... 13
7.3.44 Sample Mode - Field 70.................................................................................................................. 13
7.3.45 Spare Field - Field 71...................................................................................................................... 13
7.3.46 Spare Field - Field 72...................................................................................................................... 13
7.3.47 Sampling Error/Incomplete Aspiration Flag - Field 73 .................................................................. 14

LIST OF TABLES
TABLE I HISTOGRAM MESSAGES ................................................................................................................... 14
TABLE II COUNT DATA MESSAGE .................................................................................................................. 15
TABLE III HISTOGRAM MESSAGE EXAMPLE ............................................................................................... 17
TABLE IV COUNT DATA MESSAGE EXAMPLE............................................................................................. 23

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 NOTES

CELL-DYN® 1200 System Host Interface Specification

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1 INTRODUCTION

1.1 SCOPE
This document describes the interfacing characteristics of Abbott CELL-DYN 1200
automated hematology analyzer when attached to a Host.

1.2 DEFINITIONS AND CONVENTIONS

a) Host: external computer or data collection system.
b) System: Abbott CELL-DYN 1200.
c) The specification follows guidelines adopted at the Biomedical Instrumentation
Interface Standards Conference, University of Florida, December, 1980, and University
of Texas, Dallas, April, 1981.
d) Signal designators and related characteristics follow EIA Standard RS232C as
summarized in “The Handbook of Computers and Computing,” Seidman and Flores,
Van Nostrad Reinhold, 1984; and “Integrated Circuits Applications Handbook,” Arthur
H. Seidman, John Wiley & Sons, 1983.
e) Numeric equivalents of characters are shown as hexadecimal values.

2 MECHANICAL INTERFACE

2.1 CONNECTOR ARRANGEMENT

a) The system provides a standard DB-9 female connector mounted on the side of the
instrument.
b) Pins on the DB-9 connector:
Pin 1: Data Carrier Detect (DCD input)
Pin 2: Data to System
Pin 3: Data to Host
Pin 4: Data Terminal Ready (DTR)
Pin 5: Signal Ground
Pin 6: Data Set Ready (DSR)
Pin 7: Request to Send (RTS output)
Pin 8: Clear to Send (CTS input)
Pin 9: Ring Indicator (R.I. Input)

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3 ELECTRICAL INTERFACE
a) Voltage levels and electrical characteristics are as defined by the EIA RS-232-C
specification.
b) The maximum recommended cable length is 30 meters, or 100 feet. The actual
maximum workable cable length is dependent on the environment of the site, the
selected baud rate and the equipment being connected together.

4 DATA INTERFACE
The system can be configured for data transmission using the Computer Setup menu. The
configurable transmission parameters include data bits, stop bits, parity and baud rate.
a) The asynchronous method of data transmission (serial by bit) is used.
b) All information transmitted is in character form and is represented by 7-bit ASCII.
c) Transmitted characters consist of one (1) start bit, seven (7) or eight (8) data bits (least
significant first), one or no parity bit, and one (1) or two (2) stop bits.
d) Parity may be selected as none, odd or even.
e) The transmission speed may be selected from 1200, 2400, 4800 or 9600 bits per
second.
f) With the exception of the control characters mentioned in section 5, only printable
ASCII characters (hex 20 to hex 7E) are used in a message.

5 COMMUNICATION PROTOCOL

5.1 COMMUNICATION MODES

5.1.1 RUN MENU AUTOMATIC TRANSMIT MODE

The User may select automatic transmit mode on the Computer Setup menu. This
mode allows the automatic transmission of results (histogram data, count data or
both) during the RUN cycle.

5.2 TRANSMISSION CONTROL

Transmission control is provided in two ways: the XOFF/XON protocol, in which the Host
transmits an XOFF character (hex 13) to stop transmission from the System and an XON
character (hex 11) to re-start transmission, and CTS (clear to send) hardware control. The
XOFF/XON protocol has a 1.5 second time-out. The CTS hardware control has no time-
out. Retransmission requests are also supported and discussed in section 5.3.

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5.3 RESPONSE FROM HOST

5.3.1 BETWEEN-TRANSMISSION TIME-OUT

The time-out interval after transmission of one message is programmable in the
Computer Setup menu from 100 milliseconds to 9.9 seconds in 100-millisecond
increments.

5.3.2 RELEASE FOR NEXT MESSAGE

The Host can release the System to send the next message by sending an ACK (06
hex) before the time-out interval passes. Otherwise, the System will begin
transmission of the next message at the end of the time-out interval.

5.3.3 RETRANSMISSION
If for any reason the Host requires retransmission of the message, it signals the
System by sending a NAK (15 hex) before expiration of the time-out. A message
will be sent by the System a maximum of two (2) times. After that the same
conditions prevail as after a time-out.

5.4 MESSAGE FORMAT

: STX : ID SEGMENT : RESULT SEGMENT : CHECK SUM : ETX :

5.4.1 There are four (4) types of messages, distinguished by their ID segments.
a) WBC Histogram Message
b) RBC Histogram Message
c) PLT Histogram Message
d) Count Data Message
5.4.2 The four messages taken together represent all the results of testing a single
specimen.

5.4.3 Each message begins with STX (hex 02).

5.4.4 The Identification (ID) segment is described fully in Section 6.

5.4.5 The Result segment is described fully in Section 7.

5.4.6 The Check Sum is provided for optional processing by the Host to verify correct
transmission. It is generated by taking the module-256 sum of all the characters in
the message except the STX and ETX characters. The two-digit hexadecimal
representation of the Check Sum is placed immediately before the ETX as two
ASCII characters.
5.4.7 The ETX character (hex 03) is the last character of the message.
5.4.8 MESSAGE LENGTH
Each of the three Histogram messages is 1209 characters long. The Count Data
message is 405 characters long. These message lengths count all characters from
STX to ETX inclusive.
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 5.4.9 DATA REPRESENTATION
Numeric data are transmitted in fields of fixed length with zeros used to fill empty
spaces on the left. Out-of-range numeric values are represented by strings of '>'
characters (hex 3E), and undefined numeric values are represented by strings of '-'
characters (hex 2D). Alphanumeric data are transmitted in fields of fixed length
enclosed in double quotation marks. Within the quotation marks the data are right-
justified with blanks (hex 20) used to fill empty spaces. Fields are separated by
commas (hex 2C).

6 IDENTIFICATION (ID) SEGMENT

6.1 IDENTIFICATION
The Identification segment of each message identifies the type of message and the
specimen the message represents.

6.2 MESSAGE TYPE - FIELD 1

The Message Type is an alpha field of three (3) characters enclosed in double quotation
marks. It identifies the message. There are four message types:
a) WBC Histogram Message – Type "WBC"
b) RBC Histogram Message – Type "RBC"
c) PLT Histogram Message – Type "PLT"
d) Count Data Message – Type " "

6.3 INSTRUMENT TYPE - FIELD 2

The instrument Type is an alphanumeric field of seven (7) characters enclosed in double
quotation marks. The CELL-DYN 1200 designation is “CD1200”.

6.4 SERIAL NUMBER - FIELD 3

The instrument Serial Number is an alphanumeric field of twelve (12) characters enclosed
in double quotation marks.

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6.5 SEQUENCE NUMBER - FIELD 4
The Sequence Number is a numeric field of four (4) characters with a value ranging from 0
to 999.

6.6 SPECIMEN TYPE - FIELD 5

The Specimen Type is the fifth field in the Identification Segment. It occupies a numeric
field of one (1) character whose value gives the specimen type as follows:
0: Patient type
1: QC sample type
2: Calibrator type

6.7 OPERATOR ID - FIELD 6

The Operator ID is an alphanumeric field of three (3) characters enclosed in double
quotation marks as entered by the operator on the System.

6.8 SPECIMEN DATE - FIELD 7

The Specimen Date is an alphanumeric field of eight (8) characters enclosed in double
quotation marks, giving the date on which the specimen was run. The default format of the
date is MM/DD/YY, where MM represents the month in two digits, DD represents the day
of the month and YY represents the year.

6.9 SPECIMEN TIME - FIELD 8

The Specimen Time is a alphanumeric field of five (5) characters enclosed in double
quotation marks. It gives the time at which the specimen was run in standard 24-hour
format.

6.10 SPECIMEN ID - FIELD 9

The Specimen ID is an alphanumeric field of fifteen (15) characters with enclosing double
quotation marks. If the specimen is a patient specimen, the Specimen ID is entered by the
operator on the System. Otherwise, the Specimen ID is generated by the System to identify
special types of samples, such as controls.

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6.11 SPARE FIELD - FIELD 10
The spare field is an alphanumeric field of sixteen (16) blanks plus the enclosing quotation
marks.

6.12 SPARE FIELD - FIELD 11

The spare field is an alphanumeric field of one (1) blank plus the enclosing quotation
marks.

6.13 SPARE FIELD - FIELD 12

The spare field is an alphanumeric field of eight (8) blanks plus the enclosing quotation
marks.

6.14 SPARE FIELD - FIELD 13

The spare field is an alphanumeric field of twenty-two (22) blanks plus the enclosing
quotation marks.

6.15 SPARE FIELD - FIELD 14

The spare field is an alphanumeric field of five (5) blanks plus the enclosing quotation
marks.

6.16 SPARE FIELD - FIELD 15

The spare field is an alphanumeric field of five (5) blanks plus the enclosing quotation
marks.

6.17 SPARE FIELD - FIELD 16

The spare field is an alphanumeric field of sixteen (16) blanks plus the enclosing quotation
marks.

7 RESULTS SEGMENT
7.1 RESULTS FORMAT
The results for each specimen are sent in the format described. Refer to Table I, Table II
and the following paragraphs. All numeric fields are integers, and some need to be scaled
by the Host.

7.2 HISTOGRAM MESSAGES

7.2.1 SCALE FACTOR - FIELD 17

The Scale Factor is a numeric field of five (5) characters. It is not implemented at
this time and is therefore always transmitted as a 0.

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 7.2.2 CHANNEL DATA - FIELDS 18 THROUGH 273
The Channel Data fields are numeric fields of three (3) characters each, giving
normalized counts for every channel of the designated histogram (WBC, RBC or
PLT).

7.3 COUNT DATA MESSAGE

a) The parameters reported by the System may be represented in any of four different sets
of units, as follows:
Set 1 – Standard USA
Set 2 – SI
Set 3 – Modified SI (HGB/MCHC in mmol/L, MCH in fmol)
Set 4 – Modified SI (HCT/PCT in %)
Set 5 – International Units (WBC in 10E2/2L and RBC/PLT in 10E4/2L)
b) To convert the integer transmitted in the field corresponding to a particular parameter to
the correct value in the units being used, the decimal point must be moved leftward
from its implied position all the way to the right of the integer. The description of each
field gives a shift count indicating how many places to move the decimal point in each
case. If, for example, the decimal point is to be moved two places, a field value of
00123 becomes 1.23. All parameters in the Count Data Message are transmitted in the
Standard USA Units Set (see below).

7.3.1 WBC COUNT - FIELD 17

The WBC Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 K/2L
2–3 1 G/L
4 1 10E9/L
5 0 10E2/2L

7.3.2 SPARE FIELD - FIELD 18

The Spare Field is a numeric field of five (5) characters. It has a constant value of
zero (0) at this time.

7.3.3 LYM COUNT - FIELD 19

The LYM Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 K/2L
2–3 1 G/L
4 1 10E9/L
5 0 10E2/2L

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 7.3.4 MID COUNT - FIELD 20
The MID Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 K/2L
2–3 1 G/L
4 1 10E9/L
5 0 10E2/2L

7.3.5 GRAN COUNT - FIELD 21

The GRAN Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 K/2L
2–3 1 G/L
4 1 10E9/L
5 0 10E2/2L

7.3.6 SPARE FIELD - FIELD 22

The Spare Field is a numeric field of five (5) characters. It has a constant value of
zero (0) at this time.

7.3.7 RBC COUNT - FIELD 23

The RBC Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 2 M/2L
2–3 2 T/L
4 2 10E12/L
5 0 10E4/2L

7.3.8 HGB VALUE - FIELD 24

The HGB Value is in a numeric field, five (5) characters long.
Units Set Shift Count Units Label
1 1 g/dL
2 0 g/L
3 1 (K 0.6206) mmol/L
4 0 g/L
5 1 g/dL

7.3.9 HCT VALUE - FIELD 25

The HCT Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 %
2–3 3 L/L
4–5 1 %

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 7.3.10 MCV VALUE - FIELD 26
The MCV Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–5 1 fL

7.3.11 MCH VALUE - FIELD 27

The MCH Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–2 1 pg
3 2 (K 0.6206) fmol
4–5 1 pg

7.3.12 MCHC VALUE - FIELD 28

The MCHC Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 g/dL
2 0 g/L
3 1 (K 0.6206) mmol/L
4 0 g/L
5 1 g/dL

7.3.13 RDW VALUE - FIELD 29

The RDW Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–5 1 %

7.3.14 PLT COUNT - FIELD 30

The PLT Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 0 K/2L
2–3 0 G/L
4 0 10E9/L
5 1 10E4/2L

7.3.15 MPV VALUE - FIELD 31

The MPV Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–5 1 fL

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 7.3.16 PCT VALUE - FIELD 32
The PCT Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 2 %
2–3 1 mL/L
4–5 2 %

7.3.17 PDW VALUE - FIELD 33

The PDW Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–5 1 10(GSD)

7.3.18 SPARE FIELD - FIELD 34

The Spare Field is a numeric field of five (5) characters. It has a constant value of
zero (0) at this time.

7.3.19 LYM % VALUE - FIELD 35

The LYM % Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–5 1 %

7.3.20 MID % VALUE - FIELD 36

The MID % Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–5 1 %

7.3.21 GRAN % VALUE - FIELD 37

The GRAN % Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–5 1 %

7.3.22 SPARE FIELD - FIELD 38

The Spare Field is a numeric field of five (5) characters. It has a constant value of
zero (0) at this time.

7.3.23 MOVING AVERAGE FLAG - FIELD 39

The Moving Average Flag is in a numeric field of one character. It has not been
implemented as of this time, so it is always transmitted as 0.

7.3.24 SPARE FLAG - FIELD 40

The Spare Flag is a numeric field of one (1) character. It has a constant value of
zero (0) at this time.

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 7.3.25 R4 WBC FLAG - FIELD 41
The R4 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the flag is set, and a value of 0 indicates that it is clear.

7.3.26 GR3 WBC FLAG - FIELD 42

The GR3 WBC Flag is in a numeric field of one character. A value of 1 indicates
that the GRAN R3 flag is set, and a value of 0 indicates that it is clear.

7.3.27 MR3 WBC FLAG - FIELD 43

The MR3 WBC Flag is in a numeric field of one character. A value of 1 indicates
that the Mono R3 WBC flag is set, and a value of 0 indicates that it is clear.

7.3.28 MR2 WBC FLAG - FIELD 44

The MR2 WBC Flag is in a numeric field of one character. A value of 1 indicates
that the Mono R2 WBC flag is set, and a value of 0 indicates that it is clear.

7.3.29 LR2 WBC FLAG - FIELD 45

The LR2 WBC Flag is in a numeric field of one character. A value of 1 indicates
that the Lym R2 WBC flag is set, and a value of 0 indicates that it is clear.

7.3.30 R1 WBC FLAG - FIELD 46

The R1 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the R1 WBC flag is set, and a value of 0 indicates that it is clear.

7.3.31 R0 WBC FLAG - FIELD 47

The R0 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the flag is set, and a value of 0 indicates that it is clear.

7.3.32 SPARE FLAG - FIELD 48

The Spare Flag is a numeric field of one (1) character. It has a constant value of
zero (0) at this time.

7.3.33 STATUS FLAGS - FIELDS 49 - 54

In count data, fields 49 through 54 are status flags. The status flags are each
numeric fields of one character each. A value of 1 indicates that the flag is set. A
value of 0 indicates that it is clear. In the closed mode version 2.3, these flags have
a constant value of zero (0).

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 The status flags are as follows:

Figure 1.0
Field Status Error Represented
49 Transducer cover off
50 Lyse empty
51 Diluent empty
52 Flow error
53 Low voltage
54 Suspect RDW

7.3.34 SPARE FLAGS - FIELDS 55 THROUGH 60

There are six Spare Flag fields which are each a numeric field of one (1) character.
They each have a constant value of zero (0) at this time.

7.3.35 LRI FLAG - FIELD 61

The LRI Flag is in a numeric field of one character. A value of 1 indicates that the
flag is set, and a value of 0 indicates that it is clear.

7.3.36 URI FLAG - FIELD 62

The URI Flag is in a numeric field of one character. A value of 1 indicates that the
flag is set, and a value of 0 indicates that it is clear.

7.3.37 WBC COUNT TIME - FIELD 63

The WBC Count Time is in a numeric field five (5) characters long. It gives the
time in units of milliseconds.

7.3.38 SPARE FIELD - FIELD 64

The Spare Field is a numeric field of five (5) characters long. It has a constant
value of zero (0) at this time.

7.3.39 RBC COUNT TIME - FIELD 65

The RBC Count Time is in a numeric field five (5) characters long. It gives the
time in units of milliseconds.

7.3.40 WBC FLOW ERROR CODE - FIELD 66

The WBC Flow Error Code Field is a numeric field of five (5) characters. It
indicates in hexidecimal the type(s) of WBC flow error(s) that occurred.
(Interpretation of the flow error status is found in Figure 2.0. (RBC and WBC flow
status are interpreted individually). In the closed mode version 2.3, this flag has a
constant value of zero (0).

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 Figure 2.0
FLOW ERRORS
Cadence Voltage
Error Error
Status Cadence Voltage Voltage Voltage Low
code CV CV delta range Voltage
00
01 X
02 X
03 X X
04 X
05 X X
06 X X
07 X X X
08 X
09 X X
0A X X
0B X X X
0C X X
0D X X X
0E X X X
0F X X X X
10 X

7.3.41 RBC FLOW ERROR CODE - FIELD 67

The RBC Flow Error Code Field is a numeric field of five (5) characters It
indicates in hexidecimal the type(s) of RBC flow error(s) that occurred. (See Figure
2.0.) In the closed mode version 2.3, this flag has a constant value of zero (0).

7.3.42 SPARE FIELD - FIELD 68

The Spare Field is a numeric field of five (5) characters long. It has a constant
value of zero (0) at this time.

7.3.43 LIMITS SET - FIELD 69

The Limits Set is a numeric field of one (1) character. It has a constant value of
zero (0) at this time.

7.3.44 SAMPLE MODE - FIELD 70

The Sample Mode is a numeric field of one (1) character. For open mode
instruments, this field has a value of one (1).

7.3.45 SPARE FIELD - FIELD 71

The Spare Field is a numeric field of one (1) character. It has a constant value of
zero (0) at this time.

7.3.46 SPARE FIELD - FIELD 72

The Spare Field is a numeric field of one (1) character. It has a constant value of
zero (0) at this time.
CELL-DYN® 1200 System Host Interface Specification
03H19-01B September 2000 Page 13 of 24
 7.3.47 SAMPLING ERROR/INCOMPLETE ASPIRATION FLAG - FIELD 73
This is a numeric field of one character. A zero (0) indicates that there was no
sampling error. A one (1) indicates that there was an error.
TABLE I HISTOGRAM MESSAGES

Field # Description Length Paragraph #

1 Message Type 3# 6.2
2 Instrument Type 7# 6.3
3 Serial Number 12 # 6.4
4 Sequence Number 4 6.5
5 Specimen Type 1 6.6
6 Operator ID 3# 6.7
7 Specimen Date 8# 6.8
8 Specimen Time 5# 6.9
9 Specimen ID 15 # 6.10
10 Spare Field 16 # 6.11
11 Spare Field 1# 6.12
12 Spare Field 8# 6.13
13 Spare Field 22 # 6.14
14 Spare Field 5# 6.15
15 Spare Field 5# 6.16
16 Spare Field 16 # 6.17
17 Scale Factor 5 7.2.1
18 Channel 1 Data 3 7.2.2
... ... ... ...
273 Channel 256 Data 3 7.2.2

# indicates characters enclosed in double quotation marks

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 TABLE II COUNT DATA MESSAGE

Field # Description Length Paragraph #

1 Message Type 3# 6.2
2 Instrument Type 7# 6.3
3 Serial Number 12 # 6.4
4 Sequence Number 4 6.5
5 Specimen Type 1 6.6
6 Operator ID 3# 6.7
7 Specimen Date 8# 6.8
8 Specimen Time 5# 6.9
9 Specimen ID 15 # 6.10
10 Spare Field 16 # 6.11
11 Spare Field 1# 6.12
12 Spare Field 8# 6.13
13 Spare Field 22 # 6.14
14 Spare Field 5# 6.15
15 Spare Field 5# 6.16
16 Spare Field 16 # 6.17
17 WBC Count 5 7.3.1
18 Spare Field 5 7.3.2
19 LYM Count 5 7.3.3
20 MID Count 5 7.3.4
21 GRAN Count 5 7.3.5
22 Spare Field 5 7.3.6
23 RBC Count 5 7.3.7
24 HGB Value 5 7.3.8
25 HCT Value 5 7.3.9
26 MCV Value 5 7.3.10
27 MCH Value 5 7.3.11
28 MCHC Value 5 7.3.12
29 RDW Value 5 7.3.13
30 PLT Count 5 7.3.14
31 MPV Value 5 7.3.15
32 PCT Value 5 7.3.16
33 PDW Value 5 7.3.17
34 Spare Field 5 7.3.18

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 TABLE II COUNT DATA MESSAGE (Continued)

Field # Description Length Paragraph #

35 LYM % Value 5 7.3.19
36 MID % Value 5 7.3.20
37 GRAN % Value 5 7.3.21
38 Spare Field 5 7.3.22
39 Moving Average Flag 1 7.3.23
40 Spare Field 1 7.3.24
41 R4 WBC Flag 1 7.3.25
42 GR3 WBC Flag 1 7.3.26
43 MR3 WBC Flag 1 7.3.27
44 MR2 WBC Flag 1 7.3.28
45 LR2 WBC Flag 1 7.3.29
46 R1 WBC Flag 1 7.3.30
47 R0 WBC Flag 1 7.3.31
48 Spare Flag 1 7.3.32
49 Transducer Cover Off Status Flag 1 7.3.33
50 Lyse Empty Status Flag 1 7.3.33
51 Diluent Empty Status Flag 1 7.3.33
52 Flow Error Status Flag 1 7.3.33
53 Low Voltage Status Flag 1 7.3.33
54 Suspect RDW Status Flag 1 7.3.33
55–60 Spare Flags 1 7.3.34
61 LRI Flag 1 7.3.35
62 URI Flag 1 7.3.36
63 WBC Count Time 5 7.3.37
64 Spare Field 5 7.3.38
65 RBC Count Time 5 7.3.39
66 WBC Flow Error Code 5 7.3.40
67 RBC Flow Error Code 5 7.3.41
68 Spare Field 5 7.3.42
69 Limits Set 1 7.3.43
70 Sample Mode 1 7.3.44
71 Spare Field 1 7.3.45
72 Spare Field 1 7.3.46
Sampling Error/Incomplete Aspiration
73 1 7.3.47
Flag

# indicates characters enclosed in double quotation marks

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 TABLE III HISTOGRAM MESSAGE EXAMPLE

[STX] [start of text]

"WBC", [message type]
" CD1200", [instrument type]
"------------", [serial #]
0040, [sequence no.]
0, [specimen type]
"llc", [operator ID]
"08/31/94", [specimen date]
"12:34", [specimen time]
"123456789012345", [specimen ID]
" ", [spare field]
" ", [spare field]
" ", [spare field]
" ", [spare field]
" ", [spare field]
" ", [spare field]
" ", [spare field]
00000, [scale factor]
000, [channel 1 data]
000, [channel 2 data]
000, [channel 3 data]
000, [channel 4 data]
000, [channel 5 data]
000, [channel 6 data]
000, [channel 7 data]
000, [channel 8 data]
000, [channel 9 data]
000, [channel 10 data]
000, [channel 11 data]
000, [channel 12 data]
000, [channel 13 data]
000, [channel 14 data]
000, [channel 15 data]
000, [channel 16 data]
000, [channel 17 data]
000, [channel 18 data]
000, [channel 19 data]
000, [channel 20 data]
000, [channel 21 data]
000, [channel 22 data]
000, [channel 23 data]
000, [channel 24 data]
000, [channel 25 data]
034, [channel 26 data]
034, [channel 27 data]
034, [channel 28 data]
034, [channel 29 data]

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 TABLE III HISTOGRAM MESSAGE EXAMPLE (Continued)

054, [channel 30 data]

054, [channel 31 data]
054, [channel 32 data]
054, [channel 33 data]
080, [channel 34 data]
080, [channel 35 data]
080, [channel 36 data]
080, [channel 37 data]
093, [channel 38 data]
093, [channel 39 data]
093, [channel 40 data]
093, [channel 41 data]
080, [channel 42 data]
080, [channel 43 data]
080, [channel 44 data]
080, [channel 45 data]
071, [channel 46 data]
071, [channel 47 data]
071, [channel 48 data]
071, [channel 49 data]
063, [channel 50 data]
063, [channel 51 data]
063, [channel 54 data]
041, [channel 55 data]
041, [channel 56 data]
041, [channel 57 data]
032, [channel 58 data]
041, [channel 59 data]
029, [channel 60 data]
029, [channel 61 data]
029, [channel 62 data]
029, [channel 63 data]
029, [channel 64 data]
027, [channel 65 data]
027, [channel 66 data]
027, [channel 67 data]
027, [channel 68 data]
027, [channel 69 data]
034, [channel 70 data]
034, [channel 71 data]
034, [channel 72 data]
034, [channel 73 data]
034, [channel 74 data]
039, [channel 75 data]
039, [channel 76 data]
039, [channel 77 data]
039, [channel 78 data]

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 TABLE III HISTOGRAM MESSAGE EXAMPLE (Continued)

037, [channel 79 data]

037, [channel 80 data]
037, [channel 81 data]
037, [channel 82 data]
037, [channel 83 data]
037, [channel 84 data]
037, [channel 85 data]
041, [channel 86 data]
041, [channel 87 data]
041, [channel 88 data]
041, [channel 89 data]
046, [channel 90 data]
046, [channel 91 data]
046, [channel 92 data]
046, [channel 93 data]
046, [channel 94 data]
046, [channel 95 data]
046, [channel 96 data]
046, [channel 97 data]
046, [channel 98 data]
046, [channel 99 data]
046, [channel 100 data]
046, [channel 101 data]
049, [channel 102 data]
046, [channel 103 data]
046, [channel 104 data]
046, [channel 105 data]
054, [channel 106 data]
054, [channel 107 data]
054, [channel 108 data]
054, [channel 109 data]
058, [channel 110 data]
054, [channel 111 data]
054, [channel 112 data]
054, [channel 113 data]
063, [channel 114 data]
063, [channel 115 data]
063, [channel 116 data]
063, [channel 117 data]
066, [channel 118 data]
063, [channel 119 data]
063, [channel 120 data]
063, [channel 121 data]
063, [channel 122 data]

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 TABLE III HISTOGRAM MESSAGE EXAMPLE (Continued)

066, [channel 123 data]

066, [channel 124 data]
066, [channel 125 data]
066, [channel 126 data]
066, [channel 127 data]
066, [channel 128 data]
066, [channel 129 data]
066, [channel 130 data]
068, [channel 131 data]
068, [channel 132 data]
068, [channel 133 data]
068, [channel 134 data]
073, [channel 135 data]
068, [channel 136 data]
068, [channel 137 data]
068, [channel 138 data]
068, [channel 139 data]
078, [channel 140 data]
080, [channel 141 data]
080, [channel 142 data]
080, [channel 143 data]
080, [channel 144 data]
073, [channel 145 data]
073, [channel 146 data]
073, [channel 147 data]
073, [channel 148 data]
073, [channel 149 data]
073, [channel 150 data]
075, [channel 151 data]
083, [channel 152 data]
083, [channel 153 data]
083, [channel 154 data]
083, [channel 155 data]
083, [channel 156 data]
083, [channel 157 data]
083, [channel 158 data]
083, [channel 159 data]
088, [channel 160 data]
090, [channel 161 data]
090, [channel 162 data]
090, [channel 163 data]
090, [channel 164 data]
090, [channel 165 data]
090, [channel 166 data]
090, [channel 167 data]
090, [channel 168 data]
090, [channel 169 data]

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 TABLE III HISTOGRAM MESSAGE EXAMPLE (Continued)

095, [channel 170 data]

095, [channel 171 data]
095, [channel 172 data]
095, [channel 173 data]
110, [channel 174 data]
095, [channel 175 data]
095, [channel 176 data]
095, [channel 177 data]
110, [channel 178 data]
110, [channel 179 data]
110, [channel 180 data]
110, [channel 181 data]
110, [channel 182 data]
105, [channel 183 data]
110, [channel 184 data]
110, [channel 185 data]
110, [channel 186 data]
100, [channel 187 data]
100, [channel 188 data]
100, [channel 189 data]
100, [channel 190 data]
100, [channel 191 data]
100, [channel 192 data]
093, [channel 193 data]
085, [channel 194 data]
085, [channel 195 data]
085, [channel 196 data]
085, [channel 197 data]
085, [channel 198 data]
078, [channel 199 data]
078, [channel 200 data]
085, [channel 201 data]
085, [channel 202 data]
085, [channel 203 data]
085, [channel 204 data]
085, [channel 205 data]
085, [channel 206 data]
085, [channel 207 data]
083, [channel 208 data]
083, [channel 209 data]
083, [channel 210 data]
083, [channel 211 data]
073, [channel 212 data]

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 TABLE III HISTOGRAM MESSAGE EXAMPLE (Continued)

083, [channel 213 data]

083, [channel 214 data]
083, [channel 215 data]
083, [channel 216 data]
083, [channel 217 data]
083, [channel 218 data]
083, [channel 219 data]
083, [channel 220 data]
066, [channel 221 data]
054, [channel 222 data]
054, [channel 223 data]
054, [channel 224 data]
054, [channel 225 data]
054, [channel 226 data]
054, [channel 227 data]
051, [channel 228 data]
051, [channel 229 data]
051, [channel 230 data]
051, [channel 231 data]
051, [channel 232 data]
051, [channel 233 data]
051, [channel 234 data]
046, [channel 235 data]
034, [channel 236 data]
034, [channel 237 data]
034, [channel 238 data]
034, [channel 239 data]
034, [channel 240 data]
034, [channel 241 data]
034, [channel 242 data]
034, [channel 243 data]
034, [channel 244 data]
034, [channel 245 data]
034, [channel 246 data]
034, [channel 247 data]
027, [channel 248 data]
027, [channel 249 data]
027, [channel 250 data]
027, [channel 251 data]
027, [channel 252 data]
027, [channel 253 data]
000, [channel 254 data]
000, [channel 255 data]
000, [channel 256 data]
E7 [checksum]
[ETX] [end of text]

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03H19-01B September 2000 Page 22 of 24
 TABLE IV COUNT DATA MESSAGE EXAMPLE

[STX] [start of text]

" ", [message type]
" CD1200", [instrument type]
"------------", [serial #]
0040, [sequence no.]
0, [specimen type]
"llc", [operator ID]
"08/31/94", [specimen date]
"12:34", [specimen time]
"123456789012345", [specimen ID]
" ", [spare field]
" ", [spare field]
" ", [spare field]
" ", [spare field]
" ", [spare field]
" ", [spare field]
" ", [spare field]
00083, [WBC count]
00000, [spare field]
00015, [LYM count]
00006, [MID count]
00062, [GRAN count]
00000, [spare field]
00476, [RBC count]
00166, [HGB value]
00447, [HCT value]
00094, [MCV value]
00349, [MCH value]
00371, [MCHC value]
00118, [RDW value]
00230, [PLT value]
00084, [MPV value]
00019, [PCT value]
00174, [PDW value]
00000, [spare field]
00178, [LYM% value]
00077, [MID% value]
00745, [GRAN% value]
00000, [spare field]

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03H19-01B September 2000 Page 23 of 24
 TABLE IV COUNT DATA MESSAGE EXAMPLE (Continued)

0, [moving average flag]

0, [spare field]
0, [R4 WBC flag]
0, [GR3 WBC flag]
0, [MR3 WBC flag]
1, [MR2 WBC flag]
0, [LR2 WBC flag]
0, [R1 WBC flag]
1, [R0 WBC flag]
0, [spare field]
0, [Transducer Cover Off flag]
0, [Lyse Empty flag]
0, [Diluent Empty Flag]
0, [Flow Error flag]
0, [Low Voltage flag]
0, [Suspect RDW flag]
0, [spare field]
0, [spare field]
0, [spare field]
0, [spare field]
0, [spare field]
0, [spare field]
0, [LRI flag]
0, [URI flag]
04862, [WBC count time]
00000, [spare field]
08107, [RBC count time]
00000, [WBC Flow Error code]
00000, [RBC Flow Error code]
00000, [spare field]
0, [limits set]
0, [sample mode]
0, [spare field]
0, [spare field]
0, [sample error/incomplete aspiration]
9B, [checksum]
[ETX] [end of text]

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