8

1. Which of the following sigma specificity factors is involved in middle gene transcription during
SPO1 phage infection of bacteria?

A. g

p33
B. g

p28
C. gp34

D. gp43

E. h
ost sigma

2. Which of the following explains the events in late stage T4 infection in bacteria?

A. T

he host sigma factor specifies transcription of genes.
B. T

here is a high level of transcription of host genes.
C. Host polymerase holoenzyme directs transcription.

D. The host core enzyme participates in transcription conjunction with phage-encoded specificity

factors.
E. A
ll of the choices are correct.

3. Which of the following is mostly likely to occur when sporulatiom occurs in B. subtilis?

A. c omplete shutdown of transcription

B. a

ctivation of vegetative genes
C. deletion of vegetative genes

D. complex sigma-switching

E. r eduction of endospore formation

4. An experiment is planned to look at the transcription start site for the spoIID gene during
sporulation. Which of the following techniques would yield the most information in this
experiment?

A. s outhern analysis

B. n

orthern anlaysis
C. S1 mapping

D. run-on assays

E. D NAse footprinting


5. You are designing an experiment to investigate the presence of promoter binding sites for
holoenzymes bearing σB and σE. in B. subtilis. A DNA template is available along with the two
holoenzyme complexes and the in vitro transcription buffers. Which of the following is a suitable
negative control for this experiment?

A. a

reaction containing a DNA lacking the two promoter regions
B. a

reaction containing all the components minus UTP
C. a reaction containing DNA lacking the -10 region

D. a reaction containing DNA lacking the -35 region

E. b
oth a reaction containing a DNA lacking the two promoter regions and a reaction containing
all the components minus UTP are correct

6. O, P, S, and R genes are part of the lambda phage genome. A new clone of lambda was
constructed that lacked the O and P region. Which of the following would be observed if this new
clone were used to infect bacteria?

A. T

he clone would not enter the cell.
B. T

he clone would be destroyed by the bacterial host.
C. Replication of this would be impaired in the lytic phase.

D. A rapid burst of replication would occur.

E. T
he clone would not enter the cell and replication of this would be impaired in the lytic phase
are correct.

7. Predict the outcome of the removal of the N gene from the DNA sequence of the λ phage:

A. l ysogenic cycle will be induced.

B. e

ntry into the bacterial host will be blocked.
C. antitermination will be affected.

D. transcription from the left promoter will be interrupted.

E. a ntitermination will be affected and transcription from the left promoter will be interrupted are
correct.

8. Which of the following is true about the early phase of T7 infection of bacteria?

A. C

lass II genes are the first to be transcribed.
B. T

he host polymerase transcribes class II genes.
C. The phage-encoded polymerase transcribes the class I phage genes.

D. Class I genes are the first to be transcribed.

E. A
ll of the choices are correct.


9. Which of the following proteins is not involved the lytic phase of λ infection of bacteria?

A. C

III
B. S

10
C. NusA

D. NusG

E. N
usB

10. Which of the following is a possible mechanism for the action of NusA in termination?

A. I t binds to the RNA polymerase causing it to stall.

B. I t stimulates termination at intrinsic terminator by facilitating hairpin loop formation.

C. It binds to NusB to promote detachment of the RNA polymerase for the DNA template.

D. It interacts with S10 to promote dissociation of the RNA polymerase.

E. I t binds to the RNA polymerase causing it to stall and it stimulates termination at intrinsic
terminator by facilitating hairpin loop formation.

11. Which of the following is an incorrect statement regarding antitermination?

A. N

is involved in termination of the later genes during l phage infection.
B. A

ntitermination in the l late region requires Q.
C. Five proteins collaborate in antitermination in the l immediate early gene terminators.

D. Both N is involved in termination of the later genes during l phage infection and Antitermination

in the l late region requires Q are false.
E. N
is involved in termination of the later genes during l phage infection, Antitermination in the l
late region requires Q, and Five proteins collaborate in antitermination in the l immediate early
gene terminators are false.

12. Which of the following is involved in maintaining lysogeny in λ phage?

A. P

RE
B. P

RM
C. PRL

D. PRO

E. a
ll of the choices are correct.


13. Which of the following statements is true about the cro gene?

A. I t must be stimulated during lysogeny.

B. I ts product promotes repressor activity.

C. cro repression is important to lysogeny.

D. It is adjacent to the cIII gene.

E. I t must be stimulated during lysogeny and its product promotes repressor activity are correct.

14. Which of the following is true about the interaction with the λ repressor and RNA polymerase at
PRM?

A. I nteraction between repressor and polymerase is essential for activation of transcription.

B. I nteraction involves region 4 of σ-factor.

C. Interaction can be disrupted by the binding of CIII.

D. Interaction between repressor and polymerase is essential for activation of transcription and

interaction involves region 4 of σ-factor are correct.
E. I nteraction between repressor and polymerase is essential for activation of transcription and
interaction can be disrupted by the binding of CIII are correct.

15. Which of the following operators is a target of cI gene production?

A. O

R1
B. O

R2
C. OL1

D. OL2

E. a
ll of the choices are correct.

16. During an experiment to study the rate of infection of bacteria with T4, the bacterial cultures were
accidentally exposed to a UV light source. Which of the following would be expected for this
exposure?

A. r ecA gene is turned off.

B. C

oprotease activity in RecA protein is activated.
C. SOS response is induced.

D. Coprotease activity in RecA protein is activated and SOS response is induced are correct.

E. r ecA gene is turned off and coprotease activity in RecA protein is activated are correct.


17. Superinfection of lysogenized λ-infected E. coli is prevented because

A. t he superinfecting phage DNA is rapidly degraded by the host.

B. t he superinfecting phage DNA is inhibited by excess repressor binding.

C. the superinfecting phage cannot replicate due to insufficient nucleotide precursors.

D. the superinfecting phage DNA is rapidly degraded by the host and the superinfecting phage

DNA is inhibited by excess repressor binding are correct.
E. t he superinfecting phage DNA is rapidly degraded by the host, the superinfecting phage DNA
is inhibited by excess repressor binding, and the superinfecting phage cannot replicate due to
insufficient nucleotide precursors are correct.

18. Which of the following is a target of the Cro protein?

A. O

R2
B. O

R3
C. OL3

D. OR2 and OR3

E. O
R3 and OL3

19. ___________ ____________ bind to unfolded proteins and help them to fold properly.

________________________________________

20. PRE is the promoter used to establish _____________ in λ page.

________________________________________

21. _________________ suppression occurs when a mutation in one gene suppresses the mutation in
another gene.

________________________________________

22. When a λ lysogen suffers DNA damage, it induces the ____________ response.

________________________________________

23. Hairpin formation is necessary for ____________________.

________________________________________

24. When phage infects a bacterium, it usually subverts the host's transcription machinery to use as
its own.

True False

25. Transcription in eukaryotes is mediated by sigma factors.

True False

26. Transcriptional changes that occur when organisms respond to environmental changes are in
part due to changes in RNA polymerase.

True False

27. Heat shock genes encode proteins that lead to the rapid death of cells when they are exposed to
heat.

True False

28. T7 phage encodes a new RNA polymerase for use in the later stages of its bacterial host.

True False

29. Lysogeny is established in the production of sufficient repressor to bind to early operators and
prevent further early gene synthesis.

True False

30. UV radiation can trigger the lytic cycle in λ phage.

True False

 8 Key

1. Which of the following sigma specificity factors is involved in middle gene transcription during
SPO1 phage infection of bacteria?

A. gp33

B. gp28

C. gp34

D. gp43

E. host sigma

Blooms Level: 1. Remember

Section: 08.01
Weaver - Chapter 08 #1


2. Which of the following explains the events in late stage T4 infection in bacteria?

A. The host sigma factor specifies transcription of genes.

B. There is a high level of transcription of host genes.

C. Host polymerase holoenzyme directs transcription.

D. The host core enzyme participates in transcription conjunction with phage-encoded

specificity factors.
E. All of the choices are correct.

Blooms Level: 2. Understand

Section: 08.02
Weaver - Chapter 08 #2


3. Which of the following is mostly likely to occur when sporulatiom occurs in B. subtilis?

A. complete shutdown of transcription

B. activation of vegetative genes

C. deletion of vegetative genes

D. complex sigma-switching

E. reduction of endospore formation

Blooms Level: 2. Understand

Section: 08.01
Weaver - Chapter 08 #3



4. An experiment is planned to look at the transcription start site for the spoIID gene during
sporulation. Which of the following techniques would yield the most information in this
experiment?

A. southern analysis

B. northern anlaysis

C. S1 mapping

D. run-on assays

E. DNAse footprinting

Blooms Level: 5. Evaluate

Section: 08.01
Weaver - Chapter 08 #4


5. You are designing an experiment to investigate the presence of promoter binding sites for
holoenzymes bearing σB and σE. in B. subtilis. A DNA template is available along with the two
holoenzyme complexes and the in vitro transcription buffers. Which of the following is a
suitable negative control for this experiment?

A. a reaction containing a DNA lacking the two promoter regions

B. a reaction containing all the components minus UTP

C. a reaction containing DNA lacking the -10 region

D. a reaction containing DNA lacking the -35 region

E. both a reaction containing a DNA lacking the two promoter regions and a reaction
containing all the components minus UTP are correct

Blooms Level: 5. Evaluate

Section: 08.01
Weaver - Chapter 08 #5


6. O, P, S, and R genes are part of the lambda phage genome. A new clone of lambda was
constructed that lacked the O and P region. Which of the following would be observed if this
new clone were used to infect bacteria?

A. The clone would not enter the cell.

B. The clone would be destroyed by the bacterial host.

C. Replication of this would be impaired in the lytic phase.

D. A rapid burst of replication would occur.

E. The clone would not enter the cell and replication of this would be impaired in the lytic
phase are correct.

Blooms Level: 5. Evaluate

Section: 08.03
Weaver - Chapter 08 #7



7. Predict the outcome of the removal of the N gene from the DNA sequence of the λ phage:

A. lysogenic cycle will be induced.

B. entry into the bacterial host will be blocked.

C. antitermination will be affected.

D. transcription from the left promoter will be interrupted.

E. antitermination will be affected and transcription from the left promoter will be interrupted
are correct.

Blooms Level: 5. Evaluate

Section: 08.03
Weaver - Chapter 08 #8


8. Which of the following is true about the early phase of T7 infection of bacteria?

A. Class II genes are the first to be transcribed.

B. The host polymerase transcribes class II genes.

C. The phage-encoded polymerase transcribes the class I phage genes.

D. Class I genes are the first to be transcribed.

E. All of the choices are correct.

Blooms Level: 5. Evaluate

Section: 08.02
Weaver - Chapter 08 #9


9. Which of the following proteins is not involved the lytic phase of λ infection of bacteria?

A. CIII

B. S10

C. NusA

D. NusG

E. NusB

Blooms Level: 1. Remember

Section: 08.03
Weaver - Chapter 08 #10



10. Which of the following is a possible mechanism for the action of NusA in termination?

A. It binds to the RNA polymerase causing it to stall.

B. It stimulates termination at intrinsic terminator by facilitating hairpin loop formation.

C. It binds to NusB to promote detachment of the RNA polymerase for the DNA template.

D. It interacts with S10 to promote dissociation of the RNA polymerase.

E. It binds to the RNA polymerase causing it to stall and it stimulates termination at intrinsic
terminator by facilitating hairpin loop formation.

Blooms Level: 2. Understand

Section: 08.03
Weaver - Chapter 08 #11


11. Which of the following is an incorrect statement regarding antitermination?

A. N is involved in termination of the later genes during l phage infection.

B. Antitermination in the l late region requires Q.

C. Five proteins collaborate in antitermination in the l immediate early gene terminators.

D. Both N is involved in termination of the later genes during l phage infection and

Antitermination in the l late region requires Q are false.
E. N is involved in termination of the later genes during l phage infection, Antitermination in the
l late region requires Q, and Five proteins collaborate in antitermination in the l immediate
early gene terminators are false.

Blooms Level: 2. Understand

Section: 08.03
Weaver - Chapter 08 #12


12. Which of the following is involved in maintaining lysogeny in λ phage?

A. PRE

B. PRM

C. PRL

D. PRO

E. all of the choices are correct.

Blooms Level: 1. Remember

Section: 08.03
Weaver - Chapter 08 #13



13. Which of the following statements is true about the cro gene?

A. It must be stimulated during lysogeny.

B. Its product promotes repressor activity.

C. cro repression is important to lysogeny.

D. It is adjacent to the cIII gene.

E. It must be stimulated during lysogeny and its product promotes repressor activity are
correct.

Blooms Level: 1. Remember

Section: 08.03
Weaver - Chapter 08 #14


14. Which of the following is true about the interaction with the λ repressor and RNA polymerase
at PRM?

A. Interaction between repressor and polymerase is essential for activation of transcription.

B. Interaction involves region 4 of σ-factor.

C. Interaction can be disrupted by the binding of CIII.

D. Interaction between repressor and polymerase is essential for activation of transcription

and interaction involves region 4 of σ-factor are correct.
E. Interaction between repressor and polymerase is essential for activation of transcription
and interaction can be disrupted by the binding of CIII are correct.

Blooms Level: 2. Understand

Section: 08.03
Weaver - Chapter 08 #15


15. Which of the following operators is a target of cI gene production?

A. OR1

B. OR2

C. OL1

D. OL2

E. all of the choices are correct.

Blooms Level: 1. Remember

Section: 08.03
Weaver - Chapter 08 #16



16. During an experiment to study the rate of infection of bacteria with T4, the bacterial cultures
were accidentally exposed to a UV light source. Which of the following would be expected for
this exposure?

A. recA gene is turned off.

B. Coprotease activity in RecA protein is activated.

C. SOS response is induced.

D. Coprotease activity in RecA protein is activated and SOS response is induced are correct.

E. recA gene is turned off and coprotease activity in RecA protein is activated are correct.

Blooms Level: 5. Evaluate

Section: 08.02
Weaver - Chapter 08 #17


17. Superinfection of lysogenized λ-infected E. coli is prevented because

A. the superinfecting phage DNA is rapidly degraded by the host.

B. the superinfecting phage DNA is inhibited by excess repressor binding.

C. the superinfecting phage cannot replicate due to insufficient nucleotide precursors.

D. the superinfecting phage DNA is rapidly degraded by the host and the superinfecting phage

DNA is inhibited by excess repressor binding are correct.
E. the superinfecting phage DNA is rapidly degraded by the host, the superinfecting phage
DNA is inhibited by excess repressor binding, and the superinfecting phage cannot
replicate due to insufficient nucleotide precursors are correct.

Blooms Level: 2. Understand

Section: 08.03
Weaver - Chapter 08 #18


18. Which of the following is a target of the Cro protein?

A. OR2

B. OR3

C. OL3

D. OR2 and OR3

E. OR3 and OL3

Blooms Level: 1. Remember

Section: 08.03
Weaver - Chapter 08 #19


19. ___________ ____________ bind to unfolded proteins and help them to fold properly.

Molecular chaperones

Blooms Level: 1. Remember
 Section: 08.01
Weaver - Chapter 08 #20


20. PRE is the promoter used to establish _____________ in λ page.

lysogeny

Blooms Level: 1. Remember
Section: 08.03
Weaver - Chapter 08 #21


21. _________________ suppression occurs when a mutation in one gene suppresses the mutation
in another gene.

Intergenic

Blooms Level: 1. Remember
Section: 08.01
Weaver - Chapter 08 #22


22. When a λ lysogen suffers DNA damage, it induces the ____________ response.

SOS

Blooms Level: 1. Remember
Section: 08.03
Weaver - Chapter 08 #23


23. Hairpin formation is necessary for ____________________.

termination

Blooms Level: 1. Remember
Section: 08.03
Weaver - Chapter 08 #24


24. When phage infects a bacterium, it usually subverts the host's transcription machinery to use
as its own.

TRUE

Blooms Level: 2. Understand
Section: 08.01
Weaver - Chapter 08 #25


25. Transcription in eukaryotes is mediated by sigma factors.

FALSE

Blooms Level: 2. Understand
Section: 08.01
Weaver - Chapter 08 #26



26. Transcriptional changes that occur when organisms respond to environmental changes are in
part due to changes in RNA polymerase.

TRUE

Blooms Level: 2. Understand
Section: 08.01
Weaver - Chapter 08 #27


27. Heat shock genes encode proteins that lead to the rapid death of cells when they are exposed
to heat.

FALSE

Blooms Level: 2. Understand
Section: 08.01
Weaver - Chapter 08 #28


28. T7 phage encodes a new RNA polymerase for use in the later stages of its bacterial host.

TRUE

Blooms Level: 2. Understand
Section: 08.02
Weaver - Chapter 08 #29


29. Lysogeny is established in the production of sufficient repressor to bind to early operators and
prevent further early gene synthesis.

TRUE

Blooms Level: 2. Understand
Section: 08.03
Weaver - Chapter 08 #30


30. UV radiation can trigger the lytic cycle in λ phage.

TRUE

Blooms Level: 2. Understand
Section: 08.03
Weaver - Chapter 08 #31

 8 Summary

Category # of Questions
Blooms Level: 1. Remember 11
Blooms Level: 2. Understand 13
Blooms Level: 5. Evaluate 6
Section: 08.01 10
Section: 08.02 4
Section: 08.03 16
Weaver - Chapter 08 30