Professional Documents
Culture Documents
Genomic Libraries - PCR
Genomic Libraries - PCR
Genomic Libraries - PCR
, v ,,,-r, to tu~
1
F °
o 10 A 1..0 "><' --r tS i'n 1:-he col I e d-.:O o , Jd eQlt 'J
,~rl, ~en-ls { hie_ e nth e g<> nom e of the Ce:incem ed cA:3o.nUrn -
-fuo poe f-Xl9tO_tZoo of ~e rxJ\'n LC ( r6 o Q_r,~ , ihe -fpt::a.Q
" P A e>
u, ~ eu1 u (()
o
e ){_ t-Q c-kd
-,C:,.....,.
, ,, ~ () i\J A
o
cs
9e nom LC Dt\JA c1 Q ~ 16
1it ~ -fo, ct_ ore? ~ . The Pel.\' ti'O-Q CJJ'gt:.JiD o( rn fc of\Jf ) Jeno
ClJ\.Q.. "> --LbJ<2cfu:/ tD ~o..nc,_r,e 9el e Iec+,opho -m.; tS fa.
fv rn -/:-he_ 'fY>7'--tuxe
~e_ pw&t~on f.°9men~s ef of
Ofp --oo ~ ~ f2e,,• ihe...Je frn_c:i <Y';t'n ./s a,-i <:". --/f,.=,n ch {e \ ~
D
( p ~ ()Of' - C ~I aj
l
-.' '
)'l, , \ .J~ tf \ t0
••":I
1 I
. . ' ,, " ""\ -..-
: '-- ... ~,' \ \ \ . 0
~ '
, .-),~0 ,· ,· 1...t\_
~,,('--:' t-\ ~~\..~(''\'-
.:i 0.'lcr, l ,'{i'r o fif.' n of bo NrSa I cof o () d <onto_ l1J f'\_9 t~.
d{J) i''fe d ON Pl kn~ re) (:'() H o-f C' H'lpl l'.> / Pe l 1
'1lJ f {Qvb/ '
pro be\ f n c,d.o n~ ~Y-tb"''di2Q t.& n ~ .
0. co tonu h vbutd 1~1 0 t ~ 0 ,-l.-he bac ie nQ/ eotOn )e
~ Jn Q 7 1
ir, -{,A e Yr) ~-t(_ -.,, p fO k_ hCu_ 0 l pe cl.fi C £)N () lequ.,:,_ f; 0 '
to C lp e --l r'\e ce~ l< ,fu ft owe cl "?f foea ttn£f) t c.uf if)
p'm km e {('J d t ~ E'J, f- p n, {et hg . NOw 1---the -ft 1-1:e . ~ c
fs b a c~ct_ o_d_ ~0°c -fo f ~ -the Pt-JfJ on +he -11' (~ r
P°--peo ·
VJ ~e --Ft\ll-t~ po___p20 fs htJ b ricl t sed w trh , acivJ OO£ ve,
PY'O beA
¥ 1 he w Con reJ cohme ~ h.y 6)/ (j ( Le) LO~~h the pr26e
Is de r(_e -1:td 6cf CLt; bad. U1j 'nJ..ph ~ .
:f the f
po~ &·Lv n 0 Co 1o n ~e.t Ir:, 0-.u i:C:i b' c.d.~ J rQf ht/ c "c
,~ 0 cirit'C(
,;!- 1, ; , e-(5l me ~
lQ be nC'd o..nt~. Qrrl~ 60 cl cl oJ (ov-=E' d i:O CQ..,(y]e .
IP ()'1 & \ rOn C('.') t O (J.311 eVl ~ U--b s tm k --ID (_ (') tO ux.,_d CD r<) p:::Drcl
of
l ✓w [OU-)1 eel cDLo n 'f / () cl 1'(_o_ ~ --\t,e f()( -q> Q.,oe Cl f poxtlcu lo,1
t
1
\J --
Ise ~:-
i;y gene U6~o._"i] lDE' eo._n Q b[f' to R,Ow
.
-the 1 f.uctowf ·
1
o\ go--nr2oi~ o C5f gene. 1 fn e u to.Ye ~0 \fc c:y:. norn~.
I{ l)Se,d fo~ ch-for>)O\.Om f' 'YY\ 0-p p~ 0:]' . 0
~ seel -to ~ ~ ~~
0
J <;...h, -d
L,,C -tr, e rm u..t lt)'..,(..Un..g
- --fle - i " --!tie (;v <
~0c\eUJ\
c~ @J
l %ct (l_t ~o ~
t0- IC 0-Y1rl
_ot&
Ce_ I\
~SoL(L,t:P 0
{)
0 ~l0-~r0~d..
~ J)IJA
1 M, fol'c_U
JI f< (½ kt ~ (\
e.n~me 0 ln~~~
0
ff ~ ~l. bDfl~~ t , i
J
, J,
co
J,
Sc, e en; "'a ff ,'cien {-t +;·CA.fun
ef- rte ne)
J~
i'<'e6e ~v aJf on fu- fGo ~~( ~
we
~ c . b N f\ o_ , 6TQ~ y : -
'Ii,- c D N I') Q.j bro... 1- f.s CM ~ fc., , -fu "Ii tt-e ~ h.;_d u of. tff,s =
l:>pe c/tEc gen e .£0c t;oO f Co~ kc,c-troo ~ f'()Qte I 0
c_
~ \O-~ ~n () n IL
de~ \'~ P / I'.. I p o( y
Cl\\bo ri c c rP o t~
(J J 11 f )
U O OU. fY H d
"--, hr o l ,'3<..." d C 0 ''1 Q
lU
i r o.( YY) - R} j n.
CLC'l l \._J lQ t cc
{ ( l -~
P p r/ - lh e
(t
,R @<" JI o I ' g ~ u p { v p yoC
~ p o lm e 1r p <fQ \1 ,'d <' J Cc
ff_ ,- fu m J ~ ~ 2) I) l\_ f A
pc0 YD(? o 'o
id IS + c i 0 m e d 1 --fhe
-- o N P ; h y b
r d
l{ t { f< rf\-- fA ~
e a e
?J N o... (S
'Y ~ L t _J _ rO A /I
ntOJL V LO 1o; - R
h rs Co m p le
f o rr i2
~s O JJ 0 w h cC
U CC ~ O HJ-A)-
Corn p Ie r n e 0 ~
ON 8
:-
ir 'J O \c' go DC. -1::-a.1 lln.g
J
e a- k d GJ ~ rG
b ofd / ~ t {S i r
-to ~ l ~ R u f ) - a I\J A fy-J
1' 8--( k y p en o d e a I:{d
eJ
r - rv-.-k " o ,, J e
e n Lej m e C 7 f
J 0-
+e OfY ) ~o c &
me_ J (-- 0 i f 60 t n e to
QddA tTP _s o n e
~ Jh. tj e O l:i m e o rQ A J)'-€"'o n d
J.
i n e borh 'r 0 R '! J A {
9DD o p o { ot - (Pie
d c ¼ t i ('s, f-o~ ed
11 ., c i u rt- 1 Q
~ h o:np o 11__3 o
Ar Q
Ji en J of 6 0 !-b--Jh-e_ --:stmndJ ,.
A:-
11
i) f1 I lct1.tf" e "-. '-{d "'° L•-J! t's o.f "1Y) R.-tJ
--t--t"-t . ,. -o-dctf"{j
,
a) l Ii Ae °reO()-tf0 0
8 -in e oGq o d c -t
:J.
QQlr;e 0 o f u-t2bo ·
·f-6 Q a lf '_ d lo f t-1') oJ t
Y 'r ) ; ~ e fs
r.i
'ntJd ro ~ ~ri bo r:id,i 6 / co R!Q
1 o lu -l fo n 6 r n
1 lei
'J A ir QD?n e
f D~r~ h~b~s F gepru.o~ ·Jhe <J·h-o.ndL
0\n f\d. cl~ t ~. . n._ 0 ~ t"' l ~ ~ o cJ Cr 01r •me 11 ~ __
de o ~ ol b o ~d ,
0
"the )c 'nu ct e o ti d e..:( a9t e_
~ ' bYQ~ ~
~
, c -------
--
OH 1 -r 1 1 ,
0- r =
rr=: : : lr=:=;1=~ =:;=1~r-~=1 ~J A Fl f) A A
Ts- '
CC C C 3
er
.T T T t
-
1
3 cc c u · l
= :=
l
=::=========:J ~ fr A A- 8 CCC 3
1
t ==============
m P-N 0_ _li il l_ la i i 1 , ; !: ;i
__
3t C CCC W 5 i1 ii ii ii ii i: i_ ll l
J C O lJ F )
~r
f c, l/ g o DQ P ~
1
)
SI Cp q
c.c c.. ..--■
c;
- T i 1T 5 f
C O rJ A
~,• ! t'Lon c.op'i e.!) C:f-£rR d.M loed DN_e ~) R NiA J1 men\_ 1
~ Q D ~ Q\(0() ~ ~ Q__ 01ns\Jl Cop~
' PA ()
10
_u_
~')e
O
lnt~
C?t+-- .O·~ II
P~-
- ~ ~n c. 'YY)_a ~ -, o-f -k.w ho <...uuL
a( The PGR p"f'Ocn,yd >OJ bee n Lorr, p~-ktcd Cb-t t<>rnQW;:i
Corri pad -tt, eXY'0 o.-Q cy ct f n G.9f e a. I.J(Jj hl la In ----11-, e
MOL.9-f ~el:-- \ - - ~ ----~
... ,:
...
~
. ~
I
J
lh () ~'ef"~, r \
at ~ rie \1¥'~d -~' ( Jr )
.
u..Qncr p ~ /2 \( ?f )~ Ot
~ , J rrr r )-\ _)
,\rq t f rl r ~ ~ r ho ~ ho n,
Ct. ct eo o/l cf ~ u r r r_ r 1 1~ jr f; IG f' '1'6")
o
';J -/h e ()
~ io d in e d
1- ,i p h o s/')ho!-P)
OC IP Cdec:n
~z,
rt101p h o .~ (/ T P ) ,
0 , l n e c-hi' p h C9 sph a te ) a::, 1 t
P (d.oo ,_ '
0 rnr ( cl e [) ·).__~ o.clc. n ti
cj l l(l <{ ) (: )J (( ) e ·Mph CJ ir ha+: ) '
p-oceduJ1<? of p c R.
:-
- PH +re 2.f a s{ /- o f- PC R d
he O'fJA rfror0 wh~cA a Je rnen !
9
ef cQ paime~ rn o /e w reg 17 ~
o. n <2">lCeVl
i's -+o be ampl1\-/i ed I
of'OA p d _ Jrrf2
f ro ./' , (;:_
h ('.) J ~o--1:e.,i -(h e
d o ~ ' b01 t' d e hri p
u- e 11
e o c t,~ tY7 ;- ~ tv·Yf: ,
rJ ~ et H2o f n -th e o
1 e -n,c'f\__e d -t
o.J
S+e p Sj: :~
rs hea kc\ to Q fern p€ ra_ ~ e
1he oQCleil.,o o YD, 1--tc..vt_ e
d E :' 0 1 lo r ~ '
Q 'Ylw ie A bJ JA
- 9<?. c ) --1:h cd
0
0 °C
(( ;) ,. u o .l (d 9
Qb,o () 0 +e p 7 h 1's <J i:f' p ~
-4 cf
_-::tlJ__,\__
7hL~ fs -Lh Q_ dl_a_,n o_
~ - n lr le ~ -in e -h~s c c ~ ·
in + 0
(ie p' fl( : -
a i:t' p e ,a + u t e (Jene
Is n ow coo led --lo rn
lj lh e m h_-t u> i e
perrn ~ts o .- n n r o J /0 8 c i{ i+>-e
· ~'½f cto - G0°c_) t ha t c, In -I 0£ DN B -
ear n ~ M Q n ~ '\. e 9 w n
Poi m e , -to --t h e --+ I, f2 tu X J
{ --( h P 3 I e_ n d ,:;f
~ D (" ½ 09-l e l o
c a tf'.d o_
'Ii lh e. i l.U 1.
s ~ +c p
Je sf"N._l) t · ·7F, f
/S
/2-!cCtn clJ c> pi hQ de,, 'r w d
t a tfed A n h ea..t ,~ _
r
~4-r p- i : -
cc.-h e -t(.rnpe oO k e rs no w ~d j wt -
+hat --1-Ae .[ )1J A
S kp -<--t : ~
of -F,·ro-1.Z.o n Zs ,Y"l i'l 't oo d b ~
th e -ne t ~ ct
'Y\ " 0-r11p .i
1
<;--k p ~ :"
Pm n u1.Q.tn_8 QJ t 0w .I -fhe po i'rr, ex,; -fn 6.w p:; rrs, Lo~-f-6
bo ~h -the he: (
be r" 3
0 /~
UJ )1 0
.1 J-.an~ · Thep m-w -n o · ot
-lhei () ffi 1•3 , nc,Q TJu rn 6e 0 .
':i/ ,0 ,n dJ
f; te_ p-- c; :-
ct o Lt Ii Ec-1 -/1, e.. --nu m 6e-. q' Ccp Iej O} cl eA\ '<'e_c/ Oro A
1
)r > , ) , d
,/
,
s' - +
- -) - - - - -s '
- - -- 1
---
- -- + -
I
71 r 1J
J:.f. r ?A" I1
P01 m e·.,,r - 6 (j 0 ( )
( ?, \
'- ,. f)n noo.P :'n 9 3t
r ,J
2. {? / 7 U V
I? , , C - - - - - - - 0'
--
..; :. .
l
C.>,)
a' - - -- - - - -1 <-JO f h
~ 15 ( - ( Cr . 1 {
7C L ( l D t-J P.J
·! C L 1 < 2
l
\. I'
P o l y me:'
o ~ ,-goe
ct )
1) 1 / ✓ ll ,
~ e..g r N - f/lCZZ
t'
c:J m p l.P.
--4!2
COplsLd- C:~ d.o I C
1 d..9-no
-tu,~
----
31
-1-
--- -----
- ---J- -
t - - - -- o l
---- -----
--1--
J
r . f-lrr li'r cd t._,(\,\ cf_ rrt<? :
¥ pr R ca n be w r d +o 0-ropl[-hj· Cl. 1pPC!f--tc gq, ne f)'G'-"-leo-t
\fJ d i' t-fe~nf:- )ndrvrd(j__QV\ e~ a ~pee.)~ ( ' e\Jen ,~
c)./[k'(Q_n f:- ~o r0Cl t ~c CQ IU El>) 9o_roei_Q~ ·
. ()