Part A 

: Effect of pH on the activity of amylase in breaking down of starch

Table 1: Effects of pH on amylase activity

Time needed for Rate of amylase

colour change reaction (1/time
pH
from blue-black to needed for colour
brown (min) change)

3 11 0.09
5 3 0.33
7 1 1
9 15 0.07

pH against Rate of amylase reaction

1.2

1
Rate of amylase reaction

0.8

0.6

0.4

0.2

0
3 5 7 9
pH

Enzyme that works optimally at an acidic pH = Pepsin

Part B: Effect of substrate concentration on amylase activity

Table 2: Effect of substrate concentration of amylase activity at pH7

Amount of substrate Time needed for colour change
Amount of enzyme (mL)
(mL) from blue-black to brown (min)
1 1 1
5 1 1
10 1 2
15 1 3
20 1 9
25 1 16
 

Time needed for color change against Amount of substrate

30

25
Amount of substrate (mL)

20

15

10

0
1 2 3 9 16
Time needed for color change (min)

What happened at higher concentration of substrate that caused the rate of reaction to
become steady? 

Enzymes become saturated when the substrate concentration is high

Suggest how can you increase the rate of reaction.

Add more enzyme

Part C: Effect of enzyme concentration on amylase activity

Table 3: Effect of enzyme concentration of amylase activity

Amount of
Time needed for colour change from Rate of amylase reaction (1/time
enzyme
blue-black to brown (min) needed for colour change)
(mL)
0.2 10  0.10
0.4 8  0.13
0.6 6  0.17
0.8 4  0.25
1.0 3  0.33
1.2 3  0.33

Amount of enzyme against Rate of amylase reaction

0.35

0.3
Rate of amylase reaction

0.25

0.2

0.15

0.1

0.05

0
0.2 0.4 0.6 0.8 1 1.2
Amount of enzyme (mL)

Can the reaction still happen when no enzyme is added? Explain your answer.

The reaction still can happen but these reaction would take place slowly because enzyme are
proteins that control the speed of chemical reactions.