Page No: 20 Name of the experiment:

Exp No: 03 Preparation of 100 ml ‘Normal saline’ solution from 25% NaCl solution.
Date: 04/03/20

DEFINITION:
Normal saline is a solution of 0.9 g of NaCl present in 100 ml solution with distilled
water.
By definition Normal saline contains NaCl at a concentration of 9 g/L. It is also called
0.9% NaCl solution.

PRINCIPLE:
To prepare 100 ml of 0.9% NaCl solution (Normal saline), 0.9 g of NaCl salt is required.
To prepare the desired solution from 25% NaCl solution, we have to find out the amount of
25% NaCl solution which contains exactly 0.9 g of NaCl salt.

REAGENTS REQUIRED:
 25% NaCl solution.
 Distilled water.

APPARATUS / GLASSWEAR NEEDED:

 1 piece of 150 ml beaker.
 1 piece of 100 ml measuring cylinder (Graduated cylinder).
 1 piece of 100 ml volumetric flask.
 1 piece of 10 ml graduated pipette (with a manual ‘pipette pump’, to avoid mouth
pipetting).

CALCULATION:

In case of 25% NaCl solution,

25 g of NaCl is present in = 100 ml solution
Therefore, 0.9 g of NaCl is present in = ml of solution
= 3.6 ml of solution.

So,
3.6 ml of 25% NaCl solution is required to prepare 100 ml of 0.9% NaCl solution.
Therefore,
The required amount of distilled water to prepare this 100 ml 0.9% NaCl solution is
= (100 – 3.6) ml
= 96.4 ml
Beaker Volumetric flask Measuring cylinder

Pipette

Manual pipette pump / pipette-aid

DR. MARUFA AKHTER

Page No: 21 Name of the experiment:
Exp No: 03 Preparation of 100 ml ‘Normal saline’ solution from 25% NaCl solution.
Date: 04/03/20

PROCEDURE:
3.6 ml of 25% NaCl solution was measured by a 10 ml graduated pipette and took it a
100 ml volumetric flask, containing about 60 ml of distilled water.
The flask was shaken well and the volume was made upto 100 ml with distilled water.
Finally we had our 100 ml normal saline (0.9% NaCl solution).

MEDICAL USE OF NORMAL SALINE:

 As an intravenous infusion fluid in case of:
1. Hypotension and shock.
2. Dehydration (due to diarrgoea, vomitting or moderate to severe burn).
3. Bleeding (to a certain extent).
4. Hyponatraemia, severe hypernatraemia, DKA and HONKC
5. Maitaining intravenous chenel.
 For surgical and peritonial toileting and cleaning fresh wound.
 To treat dryness of eye as a substitute of tear.
 To dilute many injectable medications and nebulizer.
 Used with diuretics to make force douresis in case of poisoning.
 Sometimes used as intra-peritonial dialysis (IPD) fluid for IPD.

DR. MARUFA AKHTER