Worksheet 4.

1 1
Grade 9 Science – Quarter 1

Name: ________________________________ Score: ___________________

Section: ______________________________ Date Submitted: ____________

Worksheet 4.1
Leaf and Chloroplast

Objectives:
 Identify the internal structures of a leaf;
 Distinguish each part of the leaf from one another;
 Identify the parts of the chloroplast; and
 Describe the parts of the chloroplast
Overview:
The leaves of the plants are the main organs of photosynthesis. Its broad
surface enables it to absorb the light coming from the sun, which is one of the
primary requirements for photosynthesis. The leaves are also responsible to obtain
another important raw material in the photosynthetic process from the air – carbon
dioxide.
Although most leaves are thin, they are actually made up of different types of
plant cells that are vital for photosynthesis to be effectively carried out. One of the
very important components of every plant cell is the chloroplast. This is the
structure that houses the chlorophyll molecules that collect the light energy from
the sun. The cells that make up the mesophyll layers (upper and lower mesophyll
layers) of the leaves contain about 40 to 200 chloroplasts per cell. These layers are
where photosynthesis mostly occur.

Procedure:
PART A
1. Identify the leaf structure that is being described by the statements in the
table by analyzing the diagram of a cross-section of a leaf shown below.
Write your answers in the box for each part, and in the table.
2. Choose you answers from the pool of words provided below.
3. Answer the guide questions that follow.

CUTICLE

UPPER EPIDERMIS

PALISADE MESOPHYLL

LOWER EPIDERMIS

STOMA GUARD CELL

Figure 1. Cross-section of a leaf

Photo credit: https://commons.wikimedia.org/wiki/File:Leaf_anatomy.svg
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Grade 9 Science – Quarter 1

Dermis Upper epidermis Palisade mesophyll

Stomata guards Lower epidermis Guard cells
Spongy mesophyll Stoma Cuticle

Leaf
Description or Function
Structure
STOMATA Tiny openings through which the exchange of gases takes place

CUTICLE Waxy layer that reduces the rate of water loss from the leaf
surface
PALISADE Tightly packed column-shaped cells that aid in photosynthesis,
CELLS has the most amount of chloroplast
GUARD
Cells that regulate the opening and closing of the stomata
CELLS
UPPER
Epidermal layer that is found on the upper surface of the leaves
EPIDERMIS
VASCULAR Irregular-shaped loosely organized cells with air spaces, and
BUNDLES contain a few chloroplast
LOWER
Layer of the leaf structure that contain the stomata
EPIDERMIS

PART B
1. Label the parts of the chloroplast using the pool of words provided below.
Two parts are left labelled to serve as clues.
2. Identify the parts that is being described in the table below.
3. Answer the guide questions that follow.

INTERMEMBRANE OUTER MEMBRANE

SPACE

INNER
MEMBRANE

GRANUM

STROMA

Figure 2. Structure of the Chloroplast

Photo credit: https://commons.wikimedia.org/wiki/File:Figure_08_01_05.png
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Grade 9 Science – Quarter 1

Stroma Thylakoid Outer membrane

Granum Inner membrane Intermembrane space

Parts of
Chloroplas Description
t
The liquid fluid or matrix inside the chloroplast
Pancake-shaped membrane-bounded structures that contain
the chlorophyll
Outer layer that surrounds the chloroplast

Space between the two layers that enclose the chloroplast

Stack of the pancake-shaped structures

Inner layer that surrounds the chloroplast

Guide Questions:
1. What do you think, the cells that make up the upper layer of the inside of
the leaf are tightly compact and arranged side by side?
___________________________________________________________________________
___________________________________________________________________________
2. Why do you think there are air spaces between the cells that make up the
lower layer of the inside the leaf?
___________________________________________________________________________
___________________________________________________________________________
3. Why should the opening and closing of stomata be regulated?
___________________________________________________________________________
___________________________________________________________________________
4. As it was seen, there is a large number of the pancake structures in the
chloroplast that contain the chlorophyll. How is it important in the
photosynthetic process?
___________________________________________________________________________
___________________________________________________________________________
References:
Evangelista, E. V. et al. (2014). Science in Today’s World, The New Grade 9. Sibs
Publishing House, Inc. Phoenix Building 927 Quezon Avenue, Quezon City.
Hoefnagels, M. (2016). General Biology (Books I and II). McGraw-Hill
Education.
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Grade 9 Science – Quarter 1

Rabago, L. M. et al. (2014). Science and Technology 9. Vibal Group, Inc. 1253 G.
Araneta Ave., Quezon City.
https://courses.lumenlearning.com/boundless-biology/chapter/leaves/